Effect of Dietary Organic Acid Supplementation on Egg Production, Egg Quality and Some Blood Serum Parameters in Laying Hens

Transcription

1 Interntionl Journl of Poultry Sienes 7 (6): 63-62, 2008 ISSN Asin Network for Sientifi Informtion, 2008 Effet of Dietry Orgni Ai Supplementtion on Egg Proution, Egg Qulity n Some Bloo Serum Prmeters in Lying Hens M.A. Soltn Deprtment of Animl Nutrition n Clinil Nutrition, Fulty of Veterinry Meiine, University of Alexnri, Egypt Astrt: The present stuy ws rrie out to etermine the effet of ietry orgni i mixture supplementtion on performne (oy weight hnges, fee intke, fee effiieny, egg proution) egg qulity n onentrtion of some loo serum prmeters in lying hens. A totl 53 week ol white Lohmnn LSL lying hens whih were llotte into 4 groups, eh ontining 000 hens. The hens were fe with iets (7% CP% n 2795 Kl KgG ME). Supplemente with 0% (ontrol group No. ), 260 ppm (group, 2), 520 ppm (group 3) n 780 ppm (group, 4) of orgni i mixture (formi i n slt of utyri, propioni n lti is). The ietry orgni is signifintly moify live oy weight in groups 2 n 3 n non signifintly improve in group 4 when ompre with ontrol one. Orgni is supplementtion t 780 ppm (group 4) signifintly inrese egg proution y out 5.77% ompre with the untrete group, while the lower levels (groups 2 n 3) showe non signifint effet. On the other hn ietry orgni i h no effet on the verge egg weight, while showe higher perentge of smll, meium n X-lrge egg size n reution in lrge egg size ompre with ontrol group. The results revele tht no effet of orgni i on fee intke ut improvement egg mss n fee effiienies ws oserve. Higher inlusion level of orgni is (780 ppm) improve egg shell qulity n non signifintly inrese egg shell lium n protein ontent, improve yolk inex, while slightly reue lumen inex. The t revele tht liner inrese of serum lium n onentrtion of totl serum protein. From the otine t n e onluing tht orgni i supplementtion t level 780 ppm of lying hens iet improve live oy weight, improve persistene of ly n from eonomil point of view we n onlue tht orgni i ition (780 ppm) mzing inrese the eonomil effiieny of lyer proution. Key wors: Lying hens, orgni is, egg qulity, egg proution, performne, serum prmeters n eonomi effiieny Introution The poultry setor is ontinuously serhing for new fee itives in orer to improve the fee effiieny n hiken helth. The use of fee itives hs 2 ojetives, the first one is to ontrol of pthogen miroorgnisms suh s slmonell n Coliforms n the seon one is to enhne the igestive miroflor with enefiil miroorgnisms (Shne, 999). Although ntiiotis possess these enefiil effets, their use s growth promoters in the poultry inustry hs een intensively ontroversil euse of the evelopment of teril resistne n potentil onsequenes on the humn helth. Tht the reson why lterntive mterils to ntiiotis re reserhe. Among these ompouns, orgni is re promising lterntives (Hyen, 2000). Helth of the gut is one of the mjor ftors governing the performne of irs n thus, the eonomis of poultry proution (Smik et l., 2007) n the profile of intestinl miroflor plys n importnt role in gut helth (Dhwle, 2005). Dietry orgni is n their slts re le to inhiit miroorgnism growth in the foo n onsequently to preserve the miroil lne in the gstrointestinl trt. In ition, y moifying intestinl ph. Orgni is lso improve the soluility of the fee ingreients, igestion n sorption of the nutrients (Vogt et l., 98; Ptten n Wlroup, 988; Owings et l., 990; Skinner et l., 99; Ams, 999). Previous stuies reporte tht orgni is suh s fumri, propioni n utyri is n their slts hve shown vrile effets on egg proution n egg qulity prmeters. These isrepnies woul e relte to the soure, the mount of orgni is use, lotion, environmentl onition n the omposition of the iets (Jensen n Chng, 976; Gm et l., 2000). On the other hn, only few t on loo prmeters in lying hens supplemente with orgni is re ville. This stuy ws onute to through light on the effet of ifferent oses of orgni i mixture (Fumri i n lium slt of utyri, propioni n lti is) on egg proution, some egg qulity prmeters n some serum onstituents uring the lte proution stge of lying hens. Mterils n Methos This reserh ws onute t Zinh Commeril Lyer Frm in the Kingom of Sui Ari to investigte the effet of orgni i mixture prout ProviMx 63

2 M.A. Soltn: Dietry Orgni Ai Supplementtion Tle : Ingreient omposition n hemil nlysis of the Tle 2: The experimentl esign uring experimentl perios sl iet Orgni i supplementtion Items Bsl Diet (BD) Ingreients: Yellow Corn 54.0 Group No. Diet (ProviMx ommeril prout)* Orgni is level Soyen mel (48%) Bsl iet Brley 9.23 (Control) Soyen oil.0 2 BD Kg TonG BD 260 ppm Limestone BD +.0 Kg TonG BD 520 ppm DCP.5 4 BD +.5 Kg TonG BD 780 ppm DL-Methionine 0.07 Lysine 0.02 Choline hlorie 0.03 Slt 0.25 Minerl premix (0.%) 0. 2 Vitmin Premix (0.%) 0. Chemil Anlysis % Moisture.6 Crue protein 6.95 Ether Extrt 3.74 Ash 2.37 Clium 3.92 Phosphorus (Totl) ME Kl Kg 2795 : Lyer vitmin premix proue y Centrly s Co (Frne) n ontin the following vitmins per Kg premix (vitmin A, , IU; Vitmin D, IU; Vitmin E, mg; Vitmin K, 2000 mg; vitmin B2, 0 mg; Biotin, 200 mg; Folin, 000 mg; Niin, mg; pntotheni i, 0000 mg; pyrioxine, 4000 mg; riroflvin, 5000 mg; thimin, 2000 mg n proper ose of ntitoxint), 2: Minerl premix proue y Centrly s Co. (ontin the following minerls per Kg, Colt, 00 mg; Copper, 0000 mg; Iron, mg; Ioine, 500 mg; mngnese, mg; zin, mg n selenium, 200 mg). 3: Metolize energy lulte oring to NRC, 994) whih ontin mixture of (lium utyrte-lium propionte-lium Ltte n Fumri i) on egg proution, egg qulity n some loo serum iohemil prmeters in lying hens. Birs: Forty four thousn 53 weeks ol, Lohmnn LSL white lyer hens were use in this stuy. The hens were house in len well ventilte house. During the experiment, fees n wter were supplie liitum, hens were expose to 6 hours rtifiil light n the other to very im light espeilly uring the night. The housing temperture ws mintine t 24 C Experimentl esign n feeing progrm: Lying hens were llotte into 4 groups (000 hens per eh house). The lying hens were fe with sl iet ontining 7% rue protein n other nutrients oring to the requirements (NRC, 994). The ingreient omposition n hemil nlysis of the sl iets (BD) re presente in Tle,. The pplie experimentl esign is presente in Tle 2. All the experimentl iets s well s fresh wter were onstntly ville throughout the experimentl perio (6 weeks). * ProviMx is omintion of (Fumri i, 2%, slt of orgni is lium utyrte-lium propionte n lium Ltte 50% n rrier sepioliite n orn until 00% proue y Centrly s Co. Frne Dt Colletion, hemil n iohemil mesurements: Fifty hen s rnom smple were weighe t the eginning n t imonthly intervl (8 weeks) uring the experiment were reore. Egg proution ws reore ily for omputing the verge ily egg proution. Egg weight, egg size lssifition (smll, meium, lrge, X-lrge n jumo egg) ws seprte for omputing eh lss perent from the totl egg proution, moreover egg mss, fee onsumption n fee effiieny (grm fee: grm egg) were reore ily. Diet smples were ollete one for rue protein, totl sh, lium n phosphorus nlysis oring to (Rnhir n Prhn, 98; AOAC, 985; Dvi, 976; Cokerell n Holliy, 975) while, ether extrt ws etermine oring to Bligh n Dryer (959) tehnique s moifie y Hnson n Olly (963). Diets ph ws mesure using eletri igitl ph meter Orion reserh moel 20, fter ing g of the iet to 9 ml of ist. Wter n mixing for 0 min n B-vlue mesure s milliters (ml) of 0. M HCl i neee to reh ph of 5 in fee/wter mixture (0 g of mteril in 00 ml of wter). Bloo smple were ollete y venipunture from the su-wing vein n ple in non-itives loo olletion tues to proue serum from su-smple of 5 rnomly selete lying hens from eh tretment groups t the en of the experimentl perio. Serum ws seprte y entrifugtion t 3000 rpm for 0 min n nlyze for onentrtion of loo serum protein, lumin, gloulin n lium oring to Doums et l. (98), Reinhol (953), Coles (974) n Dvi (976), respetively. Egg qulity mesurements: The perent of thin shell n roken shell were reore ily. At strt n t the en of the experiment smple of 60 eggs from eh group ws ollete to estimte: Egg shpe inex (egg with/egg length), yolk inex (yolk height/yolk with), lumen inex (lumen height/(lumen length + lumen with) oring to Cr n Nesheim (972). Shell thikness n weight were reore lso; 64

3 M.A. Soltn: Dietry Orgni Ai Supplementtion Tle 3: Effet of ietry orgni i supplementtion on fee ph n B-vlue Groups No Prmeters (Control) 2 (0.5 Kg/Ton) 3 (.0 Kg/Ton) 4 (.5 Kg/Ton) Fee ph 6.53± ± ± ±0.0 Fee B-vlue Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 Tle 4: Effet of ietry orgni i on hen s oy weight (BW) hnges n mortlity uring the experimentl perio (54-) Groups No Hen s weight (Kg): 53 weeks*.86± ± ± ± weeks.65± ± ± ± ± ±0.0.8± ±0.03 BW hnge (g) Mortlity %: weeks weeks weeks Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = * oy weight efore strting the experiment moreover egg shell ws nlyze to etermine rue protein, sh, lium n phosphorus ontent. Eonomil effiieny of proution: Totl proution ost ws lulte inluing, feeing, heting, veterinry re, mngement n housing. Selling prie of the proue eggs ommonly offere in the mrket. Eonomil Effiieny (EE) ws estimte s: EE = (Net revenue/totl proution ost) 00. Sttistil nlysis: The nlysis of vrine for the otine t ws performe using Sttistil Anlysis System (SAS, 987) to ssess signifint ifferenes. Results n Disussion Fee ph n B-vlues: Effet of orgni is inlusion in lying hen iet on ph n B-vlue of the iets re presente in Tle 3. Sttistil nlysis of the otine t inite tht ietry inlusion of orgni i len of the iet non signifintly reue ph when e t 260 ppm (group No. 2) when ompre with untrete iet, while signifintly reue t 520 n 780 ppm (groups 3 n 4, respetively). On the sme wy supplementtion of orgni is reue the B-vlue of the iets oring to the inlusion levels. The ph reuing n ntimiroil effets of the orgni i my ssist in gut iifiers n pper to e goo solution for poor egg shell qulity (Diner n Butin, 2002 n Dhwle, 2005). Moreover, the B-vlue of poultry fees is prtiulrly importnt euse it ffets the ourse of igestion. When using fees with high B- vlues, the ph in the igestive trt my rise to levels unsuitle for optiml igestion n for ontrolling the teril popultion in the stomh (Lnghout, 2000; Yesilg n Colpn, 2006). Live oy weight hnge n mortlity %: Effet of ietry orgni i mixture on hen s live oy weight hnges n mortlity perent re presente in Tle 4. Sttistil nlysis of the otine t revele tht ietry orgni is signifintly moify live oy weight y out 4.59 n 4.02% in groups No. 2 n 3 whih fe on the sl iet supplemente y 260 n 520 ppm of orgni is, respetively when ompre with the ontrol, while non signifintly improve y out 2.87% in group 4 whih fe the sl iet supplemente y orgni i t 780 ppm. The results re in greement with those otine y (Gm et l., 2000) they reporte tht ietry ition of 0.05% orgni i inrese the oy weight of lying hens. It ws ler tht group 2 whih fe on the sl iet supplemente y orgni i mixture t lower level (260 ppm) showe n inrese of mortlity in the lying hens, whih my e relte to the environmentl onition of the lyer house No. 2 more thn the tretment ftor. Moreover the other inlusion rtes hve no effet on the mortlity of lying hens uring the experimentl perio. Proution hrteristis Egg proution% n egg weight : Effet of ietry orgni i supplementtion of egg proution of lying hens uring the perio etween 54- of ge is presente in Tle 5. The otine t revele tht egg proution eline within inrese of hens ge, ut orgni i supplementtion t 780 ppm (group, 4) mintin the proution t the en of the experimentl perio (70 week of hens ge) losely to the strting time of the experiment while it is lerly eline thn strting egg 65

4 M.A. Soltn: Dietry Orgni Ai Supplementtion Tle 5: Effet of ietry orgni i supplementtion on egg proution (%) of lying hens uring the experimentl perio (54-70 weeks of ge) Proution perios (weeks) (ontrol) Preliminry(53-54) 82.9± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.30 Averge (54-70) 80.64± ± ± ±0.9 Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 Tle 6: Effet of ietry orgni i supplementtion on verge egg weight (g) of lying hens uring the experimentl perio (54-70 weeks of ge) Proution perios (weeks) (ontrol) ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.82 Averge (54-70) 64.57± ± ± ±.00 Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 proution perentge of the ontrol n slightly i supplementtion h no effet of the verge egg improve in oth 260 n 520 ppm orgni is in weight ompre with the untrete one. On the other lying hens iet (group 2 n 3, respetively). Sttistil hn egg lssifition size showe signifint effet nlysis inite tht verge egg proution% for the with orgni i supplementtion n the sttistil whole perios signifintly inrese y out 5.77% of nlysis revele inrese of smll egg perentge n lying hens fe on the sl iet supplemente y 780 meium egg proution within orgni is ppm of orgni i (group, 4) when ompre with the supplementtion y out (54.2, 84.8 n 54.2%) untrete one (group, ) while the lower levels of orgni n (57.7, n 40.59%) in groups 2, 3 n 4, i supplementtion (groups, 2 n 3) showe non respetively when ompre with the ontrol, while lrge signifintly effet ompre with the ontrol. n jumo egg size showe erese perentge These results re in greement with Jensen n Chng n X lrge egg size exhiite surprising inrese of (976), Gm et l. (2000) they onlue tht orgni its proution with the higher orgni i i supplementtion hs positive effets on egg supplementtion (group, 4) when ompre with the proution in lying hens, Moreover, Yesilg n ontrol one. Colpn (2006) They oserve tht ietry orgni i Those t gree with those otine y (Jensen n supplementtion hs elerte the egg proution Chng, 976; Gm et l., 2000; Ylin et l., 2000; inrese in weeks ol lying hens ompre to Yesilg n Colpn, 2006) they inite tht the ontrol irs n extene the perio of egg ition of ifferent orgni is into lying hens iet proution, inee, week ol supplemente hens hs not signifintly moify the egg weight in lying exhiite highest lying pity thn non trete hens. Moreover itives tht ontin lti i hens. On the other hn those otine results prouing teri in lying hens iet i not influene isgree with t otine y Nollet et l. (2004) they the egg weight signifintly whih hs lrey een reporte tht positive effet of soium utyrte on reporte y (Cemigli et l., 983; Mohn et l., 995; lying performne inresing from 83.% (ontrol) to Hin et l., 996; Chen n Chen, 2004; Mhvi 83.8, 84.3, 84.8 n 86.% (50, 00, 250 n 500 ppm et l., 2005). of soium utyrte supplementtion), respetively, tht is onform the vrile effet of the itives my e Fee intke n fee effiieny: Effet of ietry orgni onfoune y vritions in gut flor n environmentl i supplementtion on egg mss, fee intke n onitions (Miles et l., 989; Mhvi et l., 2005). fee onversion rtio re presente in Tle 8. Effet of ietry orgni i supplementtion of verge Sttistil nlysis of the otine t showe tht egg weight uring the ifferent experimentl perios n orgni i supplementtion signifintly improve egg on ifferent egg lssifition size (s % from the totl mss y out 2.29 n 6.67% in lying of groups 2 n egg proution) re presente in Tle 6 n 7, 4, respetively when ompre with the untrete one, respetively. Sttistil nlysis revele tht orgni while showe non signifint improvement of group 3 66

5 M.A. Soltn: Dietry Orgni Ai Supplementtion Tle 7: Effet of ietry orgni i supplementtion on egg lssifition size (% from the totl proution) of lying hens uring the experimentl perio (54- of ge) Egg lssifition size (ontrol) Smll Egg: 6.4± ± ± ± ± ± ± ± ± ± ± ± ±0.7.59± ±0.5 Averge (54-70) 2.97± ± ± ±0.39 Meium Egg: 5.6± ± ± ± ± ± ±0.99.7± ± ± ± ± ± ± ± ±0.36 Averge (54-70) 8.77± ± ± ±0.49 Lrge Egg: 39.89± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.57 Averge (54-70) 30.90± ± ± ±0.63 X Lrge Egg: 3.35± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.80 Averge (54-70) 49.3± ± ± ±.30 Jumo Egg: 6.95± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.43 Averge (54-70) 8.6± ± ± ±0.2 Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 Tle 8: Effet of ietry orgni i supplementtion on egg mss (EM), fee intke (FI) n fee onversion rtio (FCR) of lying hens uring the experimentl perio (54- of ge) Prmeters (ontrol) EM g/hen/y): 46.35± ± ± ±.6 Perio 2 (5-62) 50.84±. 5.39± ± ± ± ± ± ± ± ± ± ±0.72 Averge(54-70) 52.44± ± ± ±0.54 FI (g/hen/y): 08.56± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.33 Averge(54-70) 08.77± ± ± ±0.7 FCR: 2.39± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.02 Averge(54-70) 2.0± ± ± ±0.02 Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 y out 0.57%. Fee intke ws omprle in the ontrol group n in the orgni i groups. Fee onversion rtio showe signifint improvement in lying hen groups whih fe on the sl iet 67

6 M.A. Soltn: Dietry Orgni Ai Supplementtion Tle 9: Effet of ietry orgni i supplementtion on physil hrter of egg shell (ES) in lying hens Thin ES (%of TEP).55± ± ± ± ±0.05.5± ± ±0.05 Broken ES(% of TEP) 0.53± ± ± ± ± ± ± ±0.02 ES thikness (mm) 0.3± ± ± ± ± ± ± ±0.06 ES weight (g) 8.26± ± ± ± ± ± ± ±0.9 *54 weeks (ge of hens t the strt of the experimentl perio). TEP = totl egg proution. Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 Tle 0: Effet of ietry orgni i supplementtion on hemil omposition (On DM sis) of egg shell (ES) in lying hens Crue Protein (%); 3.99± ± ± ± ± ± ± ±0.27 Ash (%): 90.70± ± ± ± ± ± ± ±.4 Clium (%): 9.69± ± ± ± ± ± ± ±0.54 Phosphorus (%): 0.6± ± ± ± ± ± ± ±0.008 *54 weeks (Age of hens t the strt of the experimentl perio). Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 supplemente y 260 n 780 ppm orgni i y Egg shell qulity: Effet of ietry orgni i out 3.3 n 6.9% when ompre with the untrete supplementtion on egg shell qulity, egg shell one, while group 3 whih fe on iet supplemente y thikness n egg shell weight t the strt n en of 520 ppm orgni i exhiite non signifint the experimentl perio re presente in Tle 9. improvement y out 0.95%. Sttistil nlysis of the otine t inite tht no Results regring fee intke is in greement with signifint ifferenes etween groups t the strt of the those otine y Yesilg n Colpn (2006) who experiment, while t the en of the experimentl perio inite tht ietry orgni is in lying hens i not t showe tht signifintly erese of thin egg shell, signifintly fee onsumption, while in ontrst with roken shell perentge of the totl egg proution n those otine y Smik et l. (2007) they notie tht improvement of egg thikness in lying hens groups fe use of single orgni i slt (mmonium formte or on the sl iets supplemente y 780 ppm of orgni lium propionte) in the roiler iet lowere fee is (groups No. 4) y out 50.34, n 2.5% of intke when ompre with ontrol irs, tht the teste prmeters respetively when ompre with ifferenes my e ttriute to the type of orgni i the ontrol one. On the other sie supplementtion of slt use in the stuy s in our experiment the min slt orgni is t 520 n 780 ppm (groups 3 n 4 ws utyrte in whih Skt (987), Pinhsov n respetively) non signifintly inrese egg shell Jensen (989), Leeson et l. (2005) they reporte tht weight y out 3.9 n 3.79% when ompre with utyri i, unlike other is suh s propionte, i the ontrol. not epress fee intke. The improvement of fee Effet of ietry orgni i supplementtion on effiieny were lrey emonstrte in roiler n quil hemil omposition of egg shell t the strt n en hikens (Vogt et l., 98; Ptten n Wlroup, 988; of the experimentl perio re presente in Tle 0. Ylin et l., 997; Vesteggh, 999; Denli et l., 2003). In Sttistil nlysis of the otine t inite tht lying hen Jensen n Chng (976) hve reporte there is no ifferenes of egg shell hemil enefiil effet of ietry 0.4 n 0.8% lium omposition t the strt of the experiment (54 weeks of propionte supplementtion on fee effiieny. hen s ge), while t the en of the experiment oserve 68

7 M.A. Soltn: Dietry Orgni Ai Supplementtion Tle : Effet of ietry orgni i supplementtion on internl egg qulity in lying hens Egg Inex 0.76± ± ± ± ± ± ± ±0.0 Yolk Inex 44.25± ± ± ± ± ± ± ±.2 Alumen Inex 6.23± ± ± ± ± ± ± ±0.38 *54 weeks (Age of hens t the strt of the experimentl perio). Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = 0.05 Tle 2: Effet of ietry orgni i supplementtion on the onentrtion of some loo serum prmeters of lying hens Prmeters (Control) Clium 9.38± ± ± ±0.37 Totl protein 3.98± ± ± ±0.05 Alumin.86± ± ± ±0.0 Gloulin 2.25±0..93± ± ±0.07 A/G rtio* 0.83± ± ± ±0.03 Vlues re mens ± stnr error. Mens vlues with ifferent letters t the sme row iffer signifintly t p = * A/G rtio = Alumin/Gloulin rtio. non signifintly inrese in egg shell protein ontent y ifferenes of the ifferent prmeters t the strt of the out.57, 7.52 n 7.84% n non signifintly experimentl perio, while t the en of the experiment inrese of egg shell lium ontent y out 2.66, (70 week of hen s ge) notie tht non signifint 5.98 n 5.33% in groups 2, 3 n 4 whih fe on the ifferene in egg inex n non signifintly sl iet supplemente y 260, 520 n 780 ppm eteriortion of lumen inex in lyer hens groups fe orgni i respetively when ompre with ontrol on the sl iet supplemente with ifferent inlusion group, while egg shell sh ontent showe reverse levels of orgni is when ompre with the ontrol iretion. group. While, orgni is supplementtion improve It ws oserve tht the egg shell qulity hs improve egg yolk inex. These results re in greement with whih is onsequene of the inrese minerl n protein sorption. This phenomenon of inrese (Ylin et l., 2000) they reporte signifint ifferenes sorption reflete on the inrese lium n in lumen inex, yolk inex in lyer hens fe on iet protein eposition of the shell n ontriutes to supplemente with, % lti i (Gm et l., 200), improve the qulity whih my result in reue reking stte tht slight eline of hugh unit in hens of the shells. The results re in greement with those reeiving 0.05% orgni i supplementtion. By otine y (Zeiler, 200; Roriguez-Nvrro et l., ontrst (Yesilg n Colpn, 2006) they stte tht 2002; Chen n Chen, 2004) ut isgree with those ietry orgni i hve no signifint effet on internl otine y Mhvi et l. (2005) they reporte tht egg qulity prmeters. ition of lti i prouing teri to the lying hen iet h no signifint effet on shell hrness n shell thikness. The results re unhrmonious with Yesilg n Colpn (2006) they reporte tht supplementtion of orgni i mixture in lying hens iet were not improve shell thikness n shell reking strength n tht ifferenes my e ttriute to tht orgni i mixture of their tril not inluing utyrte whih ply role in the evelopment of intestinl epithelium more thn other orgni is (Prye et l., 2002; Brons et l., 2002). Egg internl qulity: Effet of ietry orgni i supplementtion on egg inex, yolk inex n lumen inex t the strt n en of the experimentl perio re presente in Tle. The sttistil nlysis of the otine t revele tht there were no signifint 69 Bloo serum prmeters: Effet of ietry orgni i supplementtion on the onentrtion of some loo serum prmeters in lying hens t the en of the experimentl perio ( of hen s ge) re presente in Tle 2. Sttistil nlysis of the otine t revele tht liner inrese of serum lium onentrtion with the inlusion levels of orgni is in lying hen iets n signifintly inrese the serum totl protein n lumin onentrtion in lying hens of groups 3 n 4 when ompre with the ontrol one. This oul e ttriute to the fvorle environment in intestinl trt ue to feeing of orgni i, whih might hve helpe to igest n sor more nitrogen n lium. In ontrst, slight ut not signifint erese or inrese

8 M.A. Soltn: Dietry Orgni Ai Supplementtion Tle 3: Eonomi effiieny of proution s ffete y ietry orgni i supplementtion of lying hens No. of lying hens Fee Cost (USD) Other Costs USD/house)* Totl Costs (USD) Returns (USD) Net Inome (USD) T. Return/T. ost rtio (%) Net Inome/ T. ost % *Costs inluing Vet. Drugs, Egg trys, Eletri, wter, frm rent, lor n vet. Dotor slry n the reeing perio ost. of totl protein n lumin onentrtion, respetively were oserve in the group 2 whih fe on the sl iet supplemente y 0.5 Kg orgni i /ton iet. The other serum prmeters showing some vrition of serum gloulin onentrtion etween ontrol n trete irs. The results re in greement with those otine y Arsln n Sti (2004), Who onlue tht serum totl protein onentrtion signifintly inrese in Jpnese quil reeiving teri (Ltoillus ulgrius) le to proue lti i s proiotis, (Yesilg n Colpn, 2006) they reporte tht ietry orgni i supplementtion ( n.5%) signifintly inrese totl serum protein n lumin onentrtion in lying hens (Arun et l., 2006) stte tht the serum onentrtion of protein n lium inrese signifintly ue to ietry supplementtion of ltoillus sporogenes to roiler iet n (Yesilg n Colpn, 2006) they reporte tht ietry orgni i supplementtion ( n.5%) signifintly inrese totl serum protein n lumin onentrtion in lying hens. Eonomil effiieny of proution: Feeing osts, totl proution osts, net inome (USD/Group) n eonomi effiieny of proution in ifferent lying hen groups shown in Tle 3. It ws ler from the otine t tht the totl osts of lying hens proution 73408, 73746, n USD for groups -4, respetively. These results revele the possiility of inresing eonomi effiieny y orgni i supplementtion of lying hens iet. The gretest eonomil effiieny ws otine y group 4 (whih fe on the sl iet supplemente 780 ppm of orgni i) y out 23.66% when ompre with the ontrol group, while lower level of orgni is (groups 2 n 3) exhiite erese of eonomil effiieny of proution y out 4.7 n 7.47%, respetively. Conlusion: From the otine t n e onluing tht orgni i supplementtion t level 780 ppm of lying hens iet improve live oy weight, improve persistene of ly n inrese ily egg mss output, foo onversion rtio, egg shell qulity n loo serum protein n lium onentrtion uring the perio of week of hens ge. The vrile effet of orgni is inlusion of lying hen iets my e onfoune y vritions in gut flor n environmentl onition. From eonomil point of view we n onlue tht orgni i ition (780 ppm) mzing inrese the eonomil effiieny of lyer proution. Referenes Ams, C., 999. Poultry n ietry is. Fee Int., 20, 4-9: AOAC, 985. Assoition of Offiil Anlytil Chemists. Offiil Methos of Anlytil. (3th En.). Wshington DC. Arsln, C. n M. Sti, Effet of proioti ministrtion either s fee itive or y rinkking wwter on performne n loo prmeters of Jpnese quil. Arh. Geflugelk., 68: Arun, K.P., V.R. Svrm, V.L.N.R. Mnten n R.S. Sit, Dietry supplementtion of Ltoillus Sporogenes on performne n serum iohemio-lipi profile of roiler hiken. J. Poult. Si., 43: Bligh, E.G. n W.J. Dyer, 959. Ft extrtion (Cite y Person's Chemil Anlysis of Foos). (8th En.) (963). Brons, F., B. Kettlitz n E. Arrigont, Resistnt strh n the utyrte revolution. Trens Foo Si. Tehnol., 3: Cr, L.E. n M.C. Nesheim, 972. Poultry Proution. th En. Le n feiger Press, Phielphi. Cemigli, G.J., A.C. Gooling n J.A. Heert, 983. The response of lyers to feeing Ltoillus fermenttion prout. Poult. Si., 62: 399. Chen, Y.C. n T.C. Chen, Minerl utiliiztion in lyers s influene y ietry oliigofrutose n inulin. Int. J. Poult. Si., 3: Cokerell, I. n D. Holliy, 975. Qulity ontrol in-the niml feestuffs mnufturing inustry. Trop. Pro.Inst. Rep., (G97). Coles, E.H., 974. Souners Compny, Phlephi, Lonon, Torono, Vet. Clin. Pth., pp: Dvi Person, 976. The hemil nlysis of foos (7th En.). Churhill Livingstone press, Einurgh Lonon n New York. Denli, M., F. Okn n K. Celik, Effet of ietry proioti, orgni i n ntiioti supplementtion to iets on roiler performne n rss yiel. Pk. J. Nutr., 2:

9 M.A. Soltn: Dietry Orgni Ai Supplementtion Dhwle, A., Better egg shell with gut iifier. Poultry Interntionl Mgzine. Diner, J.J. n P. Butin, Use of orgni is s moel to stuy the impt of gut miroflor on nutrition n metolism. J. Appl. Poult. Res., : Doums, B.T., D.D. Byso, R. Crter, T. Peters n L.C. Shffer, 98. Determintions of totl serum protein. Clin. Chem., 27: Gm, N.M.S.Q., M.B.C. Oliver, E. Sntin n J. Berhieri, Supplementtion with orgni is in iets of lying hens. Cieni Rur., Snt Mri, 30: Hin, M.S.Y., S.M. Aulrhim, E.A.R. Hshlmoun n R.K. Roinson, 996. The effets of Ltoillus iophilus on the proution n hemil omposition of hen s eggs. Poult. Si., 75: Hnson, S.W.F. n J. Olly, 963. Ft extrtion. (Cite y Person's hemil nlysis of foos). 8th En. Hyen, M., Protete i itives. Fee Int., 7: 4-6. Jensen, L.S. n C.H. Chng, 976. Effets of lium propionte on performne of lying hens. Poult. Si., 55: Lnghout, P., New itives for roiler hikens. Worl Poult., 3: Leeson, S., H. Nmkung, M. Antongiovnni n E.H. Lee, Effet of utyri i on the performne n rss yiel on roiler hikens. J. Poult. Si., 84: Mhvi, A.H., H.R. Rhmni n J. Pourrez, Effet of proioti supplements on egg quliity n lying hen s performne. Int. J. Poult. Si., 4: Miles, R.D., A.S. Arof, R.H. Hrms, C.W. Crlson, B.L. Rei n J.S. Crwfor, 989. Effets of living non freeze-rie ltoillus iophilus ulture on performne, egg qulity n gut miroflor in ommeril lyers. Poult. Si., 60: Mohn, B., R. Kirvel, M. Bhskrn n A. Notrjn, 995. Effet of proioti supplementtion on serum/yolk holesterol n on egg shell thikness in lyers. Br. Poult. Si., 36: Nollet, L., G. Jnssens n S. Arnouts, The use of soium utyrte (Aimix utyrte C) in lyer nutrition.:nutri-a Interntionl nv. / 0/MyDouments/ Soium utyrte in lyer nutrition.pf. NRC, 994. Nutrient requirements of poultry, Ninth Revise eition, Ntionl Aemy Press. Wshington. D.C. Owings, W.J., D.L. Reynols, R.J. Hsik n P.R. Ferket, 990. Influene of ietry supplementtion with Streptoous feium M-74 on roiler oy weight fee onversion; rss hrteristis n intestinl miroil oloniztion. Poult. Si., 69: Ptten, J.D. n P.W. Wlroup, 988. The use of orgni is in roiler iets. Poult. Si., 67: Pinhsov, Y. n L.S. Jensen, 989. Effet of short hin ftty is on voluntry fee intke of roiler hiks. Poult. Si., 68: Prye, S.E., H. Dunn, G.L. Hol, C.S. Stewrt n H.J. Flint, The miroiology of utyrte formtion in humn olon., FEMS Miroiol. Lett., 27: Rnhir, S. n K. Prhn, 98. Forge evlution. First pulishe, 98 printox, New Delhi, Dhwn Printing works. Reinhol, R.R., 953. Determintion of serum lumin. Clin. Chem., 2: Roriguez-Nvrro, A., O. Klin, Y. Nys n J.M. Gri- Rui, Influene of the mirostruture on the shell strength of eggs li y hens of ifferent ges. Br. Poult. Si., 43: Skt, T., 987. Stimultory effet of short hin ftty is on epithelil for trophi effets of fermentle fier, gut miroes n luminl trophi ftors. Br. J. Nutr. 58: Smik, K.P., H. Goin, K.M. Mns n S. Gutm, Effet of orgni i slt on the performne n gut helth of roiler hiken. J. Poult. Si., 44: Shne, S., 999. The ntiiotis issue. Poult. Int., 38: Skinner, J.J., A.L. Izt n P.W. Wlroup, 99. Reserh Note: Fumri i enhnes performne of roiler hiken. Poult. Si., 70: Sttistil Anlysis System (SAS), 987. User's Guie: Sttistis SAS Institute, Cry, North Crolin. Vesteggh, H.A.J., 999. Lti i hs positive effet on roiler performne. Worl Poult., 8: 6-7. Vogt, H., S. Mthes n S. Hrnish, 98. The effets of orgni is in the rtions on the performne of roiler n lying hens. Arh. Geft., 45: Ylin, S., I. Onsilr n B. Kooglu, 997. Bilirin esisine lktik sit kullnimi.(cite in Yesilg n Colpn, 2006), Ankr Univ. Vet. FFk. Derg., 44: Ylin, S., S. Ylin, A. Sehu n K. Srifkiogullri, Yumurt tvugu rsyonlrin lktik sit kullniminin zi yumurt klite ozelliklerine etkisi. Ntionl Animl Nutrition, Congress, Isprt, Turkey. (Cite in Yesilg n Colpn, 2006), pp: Yesilg, D. n I. Colpn, Effets of orgni i supplemente iets on growth performne, egg proution n qulity n on serum prmeters in lying hens. Revue Me. Vet., 57: Zeiler, G., 200. Shell egg qulity n preservtion. 5th En. pp: