CROATIAN VETERINARY INSTITUTE

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1 CROATIAN VETERINARY INSTITUTE NRL-Salmonella Croatia Gordan Kompes

2 Outline Croatia Croatian veterinary institute NRL-Salmonella Croatia Results 2011.

3 Croatia as a part of EU Croatia signed EU accessions treaty EU accession referendum /66,25 % favor, 33,13 % against/ Croatia will become 28 th Member State of EU

4 Croatia m 2 /4,3 milion 7th century the Croats arrived in area of present-day Croatia 925. Tomislav Trpimirović kingdom of Croatia 1102 personal union of Croatia and Hungary /king Coloman 1527 Habsburg monarchy / king Ferdinand I First French Empire 1918 Kingdom of Serbs, Croats and Slovenes 1941 Germany and Italy 1945 Federal Yugoslavia Croatia

5 Croatian patents that influenced the world Necktie Thirty Years War ( ) - Croatian mercenaries in French service - Traditional small, knotted neckerchiefs Faust Vrančić parachute Ruñer Bošković atomic theory Ivan Lupis torpedo Franjo Kajfež valium Ivan Vučetić - dactyloscopy David Schwarz - zeppelin Nikola Tesla alternating current Aleksander Just and Franjo Hanaman light bulb with tungsten filament Slavoljub Penkala first mechanical pencil Slobodan Đokić, Gorjana Radoboja-Lazarevski, Zrinka Tamburašev i Gabrijela Kobrehel - azithromycin Marin Soljačić wireless energy transfer

6 Croatia - turism country

7 Continental Croatia

8 Croatian national parks

9 History of the CVI 1901 Royal Croatian and Slavonian Bacteriology Institute in Križevci 1933 Veterinary Experimental Station of the Ministry of Agriculture in Zagreb ( ) 1942 Animal Health Institute ( ) 1946 Veterinary Experimental Institute ( ) 1947 Federal Institute for Veterinary Service ( ) 1951 Yugoslav Academy of Sciences and Arts Institute for Medical Research ( ) 1952 Institute for Veterinary Medicine Research ( ) 1961 Veterinary Institute ( ) 1978 Veterinary Institutes of Croatia Veterinary Institute Zagreb ( ) 1991 Croatian Veterinary Institute Veterinary Institute Zagreb ( ) 1995 Croatian Veterinary Institute (1995 today)

10 Basic activities 1. Diagnostic and control of the infection diseases (Ministry of Agriculture, Fisheries and Rural Development) 2. Veterinary public health (Ministry of Agriculture, Fisheries and Rural Development) 3. Scientific activities (Ministry of Science, Education and Sports) 4. Market

11 Budget

12 Organisation of the CVI KRIŽEVC I CVI Zagreb Poultry center Zagreb Rijeka ZAGREB VINKOV CI Veterinary department Križevci Veterinary department Vinkovci SPLIT Veterinary department Split Veterinary department Rijeka

13 CVI - activities in methods according to the HR EN ISO / IEC 17025:2007

14 CVI organisation Laboratory for food microbiology Laboratory for residues determination Laboratory for animal feed microbiology Laboratory for chemistry Laboratory for control of veterinary medicines Laboratory for bacterial zoonoses Laboratory for general microbiology and micology Laboratory for bacterial mastitises diagnoses Laboratory for parasitology Laboratory for sterilisation and medium preparation Laboratory for swine fever, molecular virology and genetics Laboratory for rabies diagnosis and general virology Laboratory for serological diagnosis of viral diseases Laboratory for general pathology Laboratory for BSE Laboratory for fish pathology

15 Reconstruction - Before/After 1

16 Reconstruction - Before/After 2

17 Reconstruction - Before/After 3

18 Reconstruction - Before/After 4

19 Reconstruction - Before/After 5

20

21

22 The main activities of the NRL-Salmonella Croatia participation in the EURL-Salmonella workshops; participation in the EURL-Salmonella interlaboratory trials; organization of training courses and proficiency testings for the official state laboratories; testing samples from the National Salmonella Control Program; serotyping Salmonella isolates from other laboratories (official state laboratories) monitoring of resistance of Salmonella spp. isolated from poultry and swine

23 Isolation of Salmonella spp. in 2011 Poultry Center Zagreb samples Broilers (faeces/overboots) 110 Salmonella spp. Laying hens (faeces/overboots) 22 Salmonella spp. Turkey (feces/overboots) 12 Salmonella spp. Laboratory for general bacteriology 615 samples / 8 Salmonella positive (1,3 %) 6 S. Enteritidis (pig faeces) 1 S. Typhimurium (pig faeces) 1 S. Abortusequi (mare placenta) Laboratory for food microbiology 6655 samples / 195 Salmonella positive (2,9 %) chicken meat 1271 / 81 (6,4 %) turkey meat 102 / 10 (9,8 %) pork 2658 / 89 (3,4 %) beef 2219 / 11 (0,005 %) lamb 405 / 4 (0,01 %) Laboratory for animal feed microbiology 906 samples / 9 Salmonella positive (0,01 %) CVI Zagreb 495 samples /6 Salmonella positive 1 S. Chile, 1 S. Montevideo (vegetal origin raw materials), 4 S. Tennessee (feed mixtures)

24 Isolation Salmonella spp. from broilers in 2011

25 Isolation Salmonella spp. from laying hens in 2011

26 Resistance of Salmonella spp. isolated from broilers AMP 3 rd gen. ceph CHL CIP GEN NAL STR SMX TET TMP S. Enteritidis S. Mbandaka S. Infantis S. Seftenberg S. Typhimurium S. Coeln

27 Resistance of Salmonella spp. isolated from laying hens AMP 3 rd gen. ceph CHL CIP GEN NAL STR SMX TET TMP S. Enteritidis S. Mbandaka S. Infantis S. Seftenberg S. Coeln S. Tennessee One MR S.Enteritidis

28 Thank You for Your Attention

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