University Ss Cyril and Methodius in Skopje Faculty of veterinary medicine-skopje

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1 University Ss Cyril and Methodius in Skopje Faculty of veterinary medicine-skopje ACTIVITIES of the NRL-AR in Macedonia Food institute NRL AR, MK assist. prof. d-r Sandra Mojsova, Head of food and feed microbiology laboratory 12 th EURL-AR Workshop 2018" Lyngby, Denmark 5 6 April 2018

2 ФАКУЛТЕТ ЗА ВЕТЕРИНАРНА МЕДИЦИНА

3 History of FVM-Skopje Veterinary Institute - founded 1927 Veterinary Faculty founded 1994 By merging Veterinary Institute and Veterinary Faculty in Faculty of Veterinary Medicine part of University Ss. Cyril and Methodius in Skopje

4 Legislation Law on Food Safety ( Official Journal RM no. 157/10, 53/11, 1/12, 164/13, 187/13, 43/14, 72/15, 129/15). Program for monitoring and reporting on antimicrobial resistance in zoonotic and commensal bacteria Monitoring and reporting on AMR in zoonotic/commensal bacteria in animals and food (Decision 2013/652/EU). Law on Veterinary Health ( Official Journal RM no. 113/07, 24/11, 136/11, 123/12, 154/15, 53/16). Annual assignment for carrying out veterinary measures and controls for the protection of public health from contaminants or residues transmitted from animals or products of animal origin in 2018.

Food institute at the Faculty of veterinary medicine in Skopje 128 accredited methods National reference laboratory NRL AR designated by the Food and Veterinary

5 Food institute at the Faculty of veterinary medicine in Skopje 128 accredited methods National reference laboratory NRL AR designated by the Food and Veterinary Agency AR Training of personnel at the DTU National Food Institute, Denmark, EURL 2016 December EU funded Twining project - MK 12 IPA AG 01 16, Mr. Paolo Pasquali, RTA

6 2018 technical assistance (consumables - AR panels), 4 days training at FI laboratories, expert from Italy.

Dilution method: EUCAST recommends the International Organization for Standardization (ISO) reference methods For colistin testing, follow the dilution method agreed between

7 Dilution method: EUCAST recommends the International Organization for Standardization (ISO) reference methods For colistin testing, follow the dilution method agreed between CLSI and EUCAST. Laboratory protocols for isolation and identification, MIC-testing with sensititre, and reading and interpreting MIC, DTU National Food Institute, Danmark, EURL

8 We use commercially available panels from Sensititre which are designed according to (Decision 2013/652/EU). - EUVSEC 1,2 (E. coli, Salmonella) - EUCAMP(Campylobacter) - EUVENC (Enterococcus)

9 Quality Control PT participation EURL AR June 2018 Enterococcus Commensal E.coli Staphylococcus including MRSA October 2018 Salmonella Campylobacter, ESBL E.coli Reference strains Panel S. Aureus ATCC CCM 4223 DKVP/EUST E. Coli ATCC CCM 3954 EUVSEC / EUVSEC2 E. faecalis ATCC CCM 4224 EUVENC C. Jejuni ATCC CCM 6214 EUCAMP2 C. Coli EUCAMP2 E. Faecium EUVENC E. faecalis EUVENC A.Baumannii Negativ kontrol ESBL/AmpC E. coli Positiv kontrol ESBL/AmpC E. coli EUVSEC2 MacConkey kontrol MacConkey kontrol DTU National Food Institute, Danmark, EURL

10 Results Salmonella AR Origin of isolate: 12 poultry feces (NMP reduction prev. salm. poultry farm), 2 poultry meat (slaughterhouse). Randomly selected from MO bank at FVMS. Year of isolation Location- SK, SR, KR, BT, VA, TE, KU Panel: EUVSEC Species: 8 SE, 4 ST, 1 SD, 1 S spp. Interpretation: 1 CHL, AMP, TRI. threshold ECCOF interpretation Antimicrobial (epidemiological cut-off S R value) Ampicilin 13 1 >8 µg/l Azitromycin 14 NA Cefotaxime 14 >0,5µg/L Chloramphenicol 13 1 >16µg/L Ciprofloxacin 14 >0,064 µg/l Colisin 14 >2µg/L Gentamicin 14 >2µg/L Meropenem 14 >0,125µg/L Nalidixan 14 >16µg/L Sulphametoxazole 14 NA Ceftazidime 14 >2µg/L Tetracyclin 14 >8µg/L Tigecycline 14 >1µg/L Trimetophrim 13 1 >2µg/L

11 Results Campylobacter spp. AR Origin of isolate: 19 poultry ceaca and caecal swabs-scientific research PhD, 2 poultry meat. Species: Campylobacter spp. Year of isolation Panel - EUCAMP2 Interpretation: 3 CIP, STR, TET; 1 CIP, NAL, TET; 1 CIP, ERY, TET; 1 CIP, TET; 1 CYP, STR; 1 ERY, STR; 1 TET; 1 CYP Antimicrobial threshold ECCOF interpretation (epidemiological cut-off S R value) Ciprofloxacin 13 8 (61%) >0,5 µg/l Erythromycin 19 2 >4 µg/l C. coli >8µg/L Gentamicin 21 >2µg/L Nalidixic acid 20 1 >16µg/L Streptomycin 17 4 (23%) >4µg/L Tetracyclin 13 8 (61%) 1µg/L C. coli >2µg/L

12 Results Enterococcus spp. AR Origine of isolate: 4 poultry ceaca and cecal swabs, 5 traditionally produced cheese-retail. Year of isolation: poultry , cheese Location: GV, BE, PR, SK, BT Sepcies: E. faecium -6, E. faecalis -3. Panel: EUVENC Interperataton: 1 TET, TIG; 2 TET; 1 ERY, TET. threshold ECCOF interpretation Antimicrobial (epidemiological cutoff value) S R Ampicilin 9 >4 µg/l Chloramphenicol 9 >32 µg/l Ciprofloxacin 9 >4 µg/l Daptomycin 9 >4 µg/l Erythromycin 8 1 >4 µg/l Gentamicin 9 >32µg/L Linezolid 9 >4 µg/l Quinupristin/dalfop 9 >1µg; ristin E. Faecalis - NA Teicoplanin 9 >2µg/L Tetracyclin 9 4 (44%) >4µg/L Tigecycline 8 1 >0,25µg/L Vancomycin 9 >4µg/L

13 THANK YOU

Main objectives of the EURL EQAS s

EQAS Enterococci, Staphylococci and E. coli EURL workshop, April, 11 Lourdes García Migura Main objectives of the EURL EQAS s To improve the comparability of antimicrobial susceptibility testing (AST)