Laboratorio Central de Veterinaria LCV NRL Salmonella in Animals and Feed Spain. Activities to fulfill Tasks and Duties Cristina de Frutos
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1 NRL Salmonella in Animals and Feed Spain Activities to fulfill Tasks and Duties Cristina de Frutos XX Salmonella Workshop Berlin May 2015
2 NRLs Salmonella Spain Food Human Animals + Feed
3 Salmonella NRL in Animals and Feed
4 Salmonella NRL in Animals and Feed Coordinadora General de Laboratorios Salmonella NRL Administration Maintenance Quality Assurance ANIMAL HEALTH GENETICS AND CONTROL Bacteriology 1 Bees and Fish Pathology Transmissible Spongiform Encephalophaties Genetically Modified Organisms Bacteriology 2 Horses Pathology Histology Genotyping Virology 1 PCR Animal Facility Genetic Identification Virology 2 Material preparation Register Diagnostics Reagents Control Innovation and Development
5 Salmonella NRL in Animals and Feed NRL for Salmonellosis since employees at the. 7 people at the Bacteriology 1 Department: NRL- Salmonella NRL -Antimicrobial Resistance (Salmonella, Campylobacter, E.coli) NRL - Listeria NRL -Yersinia enterocolitica Accreditation according to ISO 17025: Salmonella detection :2009 Salmonella spp Serotyping: 2010 Antimicrobial Resistance :2012
6 Salmonella NRL in Animals and Feed NRL for zoonoses in feed and animals : Campylobacter, Listeria, VTEC, Botulism, Vibrio, Yersinia enterocolitica. NRL Salmonella in Animals and Feed NRL Antimicrobial Resistance (Salmonella, Campylobacter, E.coli) NRL Fish Diseases NRL Aujeszky NRL Avian Influenza NRL Newcastle NRL Aftosa Virus NRL Swine Vesicular Disease NRL Bluetongue NRL African and Classical Swine Fever NRL Enzootic Bovine Leukosis NRL African Horse Sickness EURL African Horse Sickness (DOCCEE 10/6/92) OIE African Horse Sickness (10/3/93)
7 Network of veterinary laboratories working in Salmonella National Control Programs Official Laboratories NRL 20 Official Laboratories
8 Network of veterinary laboratories working in Salmonella National Control Programs Business Operators Laboratories NRL 20 Official Laboratories 30 Business Operators Labs
9 Network of laboratories working in Salmonella National Control Programs (NCP) Official labs are designated by the competent authority in each Autonomous Community: Accreditation according to ISO Detection Methods: ISO Annex D Business operators labs are approved by the Autonomous Community where located: Accreditation according to ISO Detection Methods: ISO 6579 Annex D Authorised alternative methods Validated against the reference method- ISO Registered in Ministry of Agriculture.
10 Activities of NRL-Salmonella - Coordination of Laboratories NCP (I) Harmonization and updating of information for the correct implementation of National Control Programs. Samples (preparation, acceptance of samples ) Approved live vaccines Methods to distinguish from the wildtype strains Correct reporting of results trough the Ministry Database. Authorised alternative Laboratory Methods: Vidas Up Salmonella - BioMerieux IQ-Check TM Salmonella II - Bio-Rad QFast Salmonella - imicroq Micro SEQ Salmonella spp Life Technologies(in process) Updating about prevalence and progress of Salmonella Programs in the different populations.
11 Activities of NRL-Salmonella - Coordination of Laboratories NCP (II) Provide Technical assistance for accreditation purposes. Dissemination of the information provided by the EURL: New ISO guidelines Protocols ( PCR monophasic S.Typhimurium ) Newsletters Organization of annual meetings with the labs taking part in the National Control Programs.
12 Activities of NRL-Salmonella - Interlaboratory Comparison Studies (I) Interlaboratory Comparison Studies on the Detection of Salmonella spp 2 studies/year on Avian faeces (since 2002) Labs taking part: 20 official labs, 30 business operators labs. Number of samples: 15 Reference Material Freeze dried strains Lenticules Spiked samples 1 study/year on Feed (since 2004) Labs taking part: 23 official labs Number of samples: 13 Reference material: Freeze dried strains Follow-up of laboratories with poor performance
13 Activities of NRL-Salmonella - Interlaboratory Comparison Studies (II) Interlaboratory Comparison Studies on Salmonella Serotyping 1 study/year (since 2008) Labs taking part : 35 (official + business operators) Number of samples : 15 Salmonella strains PCR for confirmation of monophasic S. Typhimurium included
14 Activities of NRL-Salmonella - Training for Laboratories working on NCP After poor performance at interlaboratory studies. On request. Since 2004: Training on detection and identification of Salmonella spp. 1 official lab 4 business operators labs Training on serotyping of Salmonella spp. 9 official labs 25 business operators labs
15 Activities of NRL-Salmonella - Other Activities of the on Salmonellosis (I) Bacteriological and Serological test for import and export (horses,cocks.) ISO 6579:2002 / ISO 6579:2002/Amd 1:2007 Serum agglutination test. Salmonella Characterization Serotyping aprox 1500 Salmonella strains/year (coming from NCP, other activities in official labs, universities etc) Agglutination according to White Kauffmann - LeMinor. Multiplex PCR : Research in Microbiology 158(2007) ; Journal of Clinical Microbiology June 2004, p ; Research in Microbiology 153 (2002) PFGE -Xbal
16 Activities of NRL-Salmonella - Other Activities of the on Salmonellosis (II) Maintenance of Salmonella collection (aprox strains) Collaboration with the Ministry of Agriculture: Registration of alternative Salmonella detection methods. Implementation and management of Databases for Salmonella National Control Programs. Participation in Salmonella working groups with Autonomous Communities. Monitoring and reporting of AMR on Salmonella strains coming from the national control programmes (Commission Decision 2013/652)
17 Activities of NRL-Salmonella - Other Activities of the on Salmonellosis (III) Collaboration in research activities with other official institutions and universities. J. Hidalgo-Vila, C. Díaz-Paniagua, C. de Frutos-Escobar, C. Jiménez-Martínez, N.Pérez-Santigosa. Salmonella in free living terrestrial and aquatic turtles. Veterinary Microbiology 119 (2007) J. Hidalgo-Vila, C.Díaz-Paniagua, X. Ruiz, A. Portheault, H. El Mouden, T. Slimani, C. de Frutos, M.S. de Caso. Salmonella species in free-living spur-thighed tortoises (Testudo graeca) in central western Morocco. Veterinary Record (2008) 162, J. Hidalgo-Vila, C. Díaz-Paniagua, N.Pérez-santigosa, C.de Frutos-Escobar, A.Herrero-Herrero. Salmonella in free-living exotic and native turtles and in pet exotic turtles from SW Spain. Research in Veterinary Science 85 (2008) Gregorio J.Torres, F.Javier Piquer, Leonor Algarra, Cristina de Frutos, Odón J.Sobrino. The prevalence of Salmonella enterica in Spanish feed mills and potential feed-related risk factors for contamination. Preventive Veterinary Medicine 2011 Feb1; 98(2-3):81-7. Victoria Garrido, Samanta Sáncjhez, Beatriz San Román, Ana Zabalza-Baranguá, Yasmín Díaz-Tendero, Cristina de Frutos, Raúl-Carlos Mainar Jaime, María-Jesus Grilló. Simultaneous infections by different Salmonella strains in mesenteric lymph nodes of finishing pigs. BMC Veterinary Research 2014, 10:59 Sara Andrés Barranco, Juan P. Vico,Victoria Garrido, Sofía Samper, Silvia Herrera-León, Cristina de Frutos RaúlC.Mainar jaime. Role of Wild Bird and Rodents in the Epidemiology of subclinical Salmonellosis in finishing pigs. Foodborne Pathogens and Disease. Volume 0, Number 0,2014
18 Thank you for your attention
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