OIE Reference Laboratory Reports Activities

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1 OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : :30:02 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Salmonellosis Address of laboratory: New Haw, Addlestone Surrey KT15 3NB Weybridge UNITED KINGDOM Tel.: Fax: address: rob.davies@ahvla.gsi.gov.uk website: Name (including Title) of Head of Laboratory (Responsible Official): Prof. Glyn Hewinson Name (including Title and Position) of OIE Reference Expert: Dr. Robert H. Davies Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally SAT (S. Gallinarum/Pullorum) yes 52 SAT (S. Abortusequi) yes 196 SAT (S. Dublin) yes 64 SAT (S. Typhimurium) yes 26 Direct diagnostic tests Nationally Internationally Serotyping yes Phagetyping yes Antimicrobial susceptibility testing yes Tests for live Salmonella vaccines yes 164 Salmonella culture yes 9943 ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents 2 OIE Reference Laboratory Reports Activities, 2013

3 to OIE Member Countries? Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Salmonella typing sera serotyping 14,560 ml produced 25,302 ml 600 ml commercially sensitive information Asia and Pacific Middle East S. Pullorum control serum SAT 4 ml provided 4 ml commercially sensitive information Asia and Pacific Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries OIE Reference Laboratory Reports Activities,

4 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided SAUDI ARABIA General advice on Salmonella control in breeding chickens Remote assistance BELGIUM Advice on sampling techniques for pigs Remote assistance BRAZIL Advice on optimising sampling techniques according to OIE recommendations Remote assistance PORTUGAL Advice on optimising PCR techniques to differentiate ST and monophasic strains of ST Remote assistance TURKEY Advice on detection of Salmonella by real-time PCR Remote assistance IRAQ General advice on sampling and testing methods Remote assistance SWEDEN Advice on invasiveness of Salmonella strains Remote assistance AUSTRALIA Advice on testing scheme to eradicate S. Pullorum Remote assistance UNITED KINGDOM Advice on outbreak control to the state of Jersey Remote assistance ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 4 OIE Reference Laboratory Reports Activities, 2013

5 Title of the study Duration Purpose of the study Partners (Institutions) Molecular serotyping study 1 year Evaluation of molecular methods for serotyping Salmonella various Molecular serotyping study 1 year Evaluation of molecular methods for serotyping Salmonella various Salmonella vaccine trial 2 years Field study, Salmonella in pigs IDT Biologica, BPEX DISCONTROLS 2 years Collection and ranking of disease control and infection various DISCONTROLS 2 years Collection and ranking of disease control and infection various DISCONTROLS 2 years Collection and ranking of disease control and infection various S. Java study 1 year Molecular analysis and comparison of S. Java strains Veterinary and Agrochemical Research Centre S. Java study 1 year Molecular analysis and comparison of S. Java strains Veterinary and Agrochemical Research Centre ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: ARNOLD, M. E.; MARTELLI, F.; MCLAREN, I.; & DAVIES, R. H. (2013). Estimation of the Rate of Egg Contamination from Salmonella Infected Chickens. Zoonoses and public health. Feb 7. doi: /zph [Epub ahead of print] 2. ARNOLD, M. E.; MARTELLI, F.; MCLAREN, I.; & DAVIES, R. H. (2013). Estimation of the sensitivity of environmental sampling for detection of Salmonella in commercial layer flocks post-introduction of national control programmes. Epidemiol. Infect.,. Cambridge University Press 2013 doi: /s [Epub ahead of print] 3. BRUNTON, L. A.; CLIFTON-HADLEY, F. A.; SHEEHAN, R.; HILL, A.; MARIER, E.; DAVIES, R. H.; TURVEY, K. & COOK, A. J. C. (2013). Expert opinion on Salmonella control in pigs in the UK. The Pig Journal 68, OIE Reference Laboratory Reports Activities,

6 4. DAVIES, R. ; DEUCHANDE, R. ; LARKIN, L. ; COLLINS, R. & IRVINE, R. M. (2013). Multidrug resistant Salmonella Java found in British broiler flocks. Veterinary Record, 172 (23), DAVIES, R.H. & WALES, A.D. (2013). Salmonella contamination of cereal ingredients for animal feeds, Veterinary Microbiology 166, HORTON, R.A.; WU, G.; SPEED, K.; KIDD, S.; DAVIES, R. H.; COLDHAM, N. G. & DUFF, J.P. (2013). Wild birds carry similar Salmonella enterica serovar Typhimurium strains to those found in domestic animals and livestock. Research in Veterinary Science. 95 (1), JONES, E. M; SNOW, L.C.; CARRIQUE-MAS, J.J.; GOSLING, R.J.; CLOUTING, C. & DAVIES, R.H. (2013). Risk factors for antimicrobial resistance in Escherichia coli found in GB turkey flocks Veterinary Record 2013;173: Published Online First: 3 October MUELLER-DOBLIES, D.; SPEED, K. & DAVIES, R. (2013). A retrospective analysis of Salmonella serovars isolated from pigs in Great Britain between 1994 and Preventive Veterinary Medicine, 110, MUELLER-DOBLIES, D.; CLOUTING, C. & DAVIES, R. (2013). Investigations of the Distribution and Persistence of Salmonella and Ciprofloxacin-Resistant Escherichia coli in Turkey Hatcheries in the UK. Zoonoses and Public Health. 60, WALES, A.; MCLAREN, I.; RABIE, A.; GOSLING, R.; MARTELLI, F.; SAYERS, R.; DAVIES, R. (2013). Assessment of the anti-salmonella activity of commercial formulations of organic acid products. Avian Pathology, 42, WALES, A. & DAVIES, R. H. (2013). Salmonella contamination of cereal ingredients for animal feeds. Veterinary Microbiology,166, WALES, A.; WEAVER, J.; MCLAREN, I; SMITH, R.P.; MUELLER-DOBLIES, D. & DAVIES, R. H. (2013). Investigation of the Distribution of Salmonella within an Integrated Pig Breeding and Production Organisation in the United Kingdom, ISRN Veterinary Science. Volume 2013 (2013), Article ID , 6 pageshttp://dx.doi.org/ /2013/ b) International conferences: ARNOLD, M., GOSLING, R.J., DAVIES, R.H. (2013) Evaluation of the sensitivity of faecal sampling for detection of monophasic ST and other Salmonella in cattle and pigs. Proc. ECVPH Conference, Sept. 2013, Turin, Italy. P COOK, A. J. C. & DAVIES, R. H. (2013) Detecting Salmonella in pigs at slaughter - a comparison of sample types.. Proceedings of the Joint Meeting of the 5th an Symposium of Porcine Health Management and the 50th Anniversary Meeting of the Pig Veterinary Society of Great Britain, Edinburgh, UK, 22nd - 24th May 2013 Page: CRAYFORD, G. & DAVIES, R. (2013) Observations on the latest epidemic strain of Salmonella in pigs: monophasic S. Typhimurium. Proceedings of the Joint Meeting of the 5th an Symposium of Porcine Health Management and the 50th Anniversary Meeting of the Pig Veterinary Society of Great Britain, Edinburgh, UK, 22nd - 24th May 2013 Page: DAVIES, R. (2013) Salmonella control in food animals in the EU: successes, threats and future challenges. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May DAVIES, R. and MUELLER-DOBLIES, D. (2013) Observations on the occurrence and persistence of monophasic Salmonella Typhimurium (S.4, [5],12:i:-) in poultry farms.. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May DAVIES, R. (2013) Investigation of low prevalence Salmonella contamination in poultry feedmills. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May DAVIES, R.H; GOSLING, R.J., SMITH, R.P. (2013) - Results from a UK Salmonella Vaccine Study. Proc. 10th International Conference on the Epidemiology and Control of Foodborne Pathogens and Antimicrobial Resistance in Pigs and Pork, Sept. 9-12, Portland, Maine, USA. 8. GOSLING, R.J., SAYERS, R., DAVIES, R.H. (2013) Investigation of laboratory testing issues in the context of the 6 OIE Reference Laboratory Reports Activities, 2013

7 Salmonella National Control Programme in GB. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May GOSLING, R.J.& DAVIES, R.H. (2013) A longitudinal study on the persistence of non-avian Salmonella Enteritidis on cattle farms in the UK. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May GOSLING, R.J., MARTELLI, F., WINTRIP, A., SAYERS, R., WHEELER, K., BEECHENER, S., DAVIES, R.H. (2013) Assessment of the social science aspect of biosecurity and uptake of advice on chicken layer farms in England and Wales. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May GOSLING, R.J., MARTELLI, F., WINTRIP, A., SAYERS, A.R., WHEELER, K., BEECHENER, S. DAVIES, R.H.(2013) On farm Salmonella control why, when & how? Proc. ECVPH Conference, Sept. 2013, Turin, Italy. P LAWES, J.R., CHENEY, T., MUELLER-DOBLIES, D. & DAVIES, R. (2013) Results from Salmonella National Control Programme in chicken broiler flocks in GB: SVEPM Conference, Madrid, Spain, March MARTELLI F, GOSLING R, KENNEDY E, RABIE A, REEVES H, CLIFTON-HADLEY F, DAVIES R and LA RAGIONE R (2013) Invasiveness of monophasic and aphasic Salmonella strains in point of lay chickens. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May MARTELLI F, GOSLING R, KENNEDY E, RABIE A, BRESLIN M, DAVIES R and LA RAGIONE R (2013) Evaluation of protection provided by an inactivated Salmonella vaccine in point-of-lay chickens challenged with monophasic Salmonella Typhimurium strains. Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May MARTELLI F, GOSLING R, MCLAREN I, SAYERS R and DAVIES R (2013) Sensitivity of different sampling methods in the detection of Salmonella in flocks of laying hens. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May MARTELLI F, GOSLING R, MCLAREN I, RABIE A and DAVIES R (2013) Salmonella- contaminated eggs in flocks identified as positive by the National Control Programme for Salmonella in the UK. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May MARTELLI F, GOSLING R, WINTRIP A, WHEELER K, BEECHENER S, SAYERS R and DAVIES R (2013) Assessment of biosecurity and uptake of advice in chicken layer farms in England and Wales. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May MARTELLI F, LA RAGIONE R. and DAVIES R. Egg contamination with Salmonella Typhimurium and its monophasic variants. Proceedings of the XXI an Symposium on the Quality of Poultry Meat and XXV an Symposium on the Quality of Eggs and Egg products, World s Poultry Science Journal, Volume 69, page MUELLER-DOBLIES, D. and DAVIES, R. (2013). A study of the dynamics of Salmonella infection in turkey breeding, rearing and finishing houses with special reference to elimination, persistence and introduction of Salmonella. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May MUELLER-DOBLIES, D. and DAVIES, R. (2013). Salmonella serovars and phage types associated with specific animal reservoirs and their epidemiology situation in the United Kingdom. In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May PETROVSKA, L. (2013). Unifying the epidemiological and population dynamics of the monophasic Salmonella infections using whole genome sequencing. Med-Vet-Net Association International Scientific Conference. DTU, Lyngby, Denmark, June RABIE A, McLAREN I, BRESLIN M, MARTELLI F and DAVIES R (2013) Assessment of anti-salmonella activity of boot dip samples In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May OIE Reference Laboratory Reports Activities,

8 23. RABIE A, McLAREN I, SAYERS R, MARTELLI F, VAUGHAN and DAVIES R (2013) Study on the effect of storage conditions on the recovery of Salmonella from boot swabs and hairnets In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May RABIE A, McLAREN I, MARTELLI F AND DAVIES R (2013) A comparison between Rambach agar, Rapid Salmonella Complete agar and Brilliance Salmonella agar for detection of Salmonella In: Proceedings of I3S International Symposium Salmonella and Salmonellosis, St Malo, France, May c) National conferences: 0 d) Other: (Provide website address or link to appropriate information) 3 1. ANON (2013). Scientific Opinion on the evaluation of molecular typing methods for major food-borne microbiological hazards and their use for attribution modelling, outbreak investigation and scanning surveillance: Part 1 (evaluation of methods and applications). EFSA Journal 2013;11(12):3502, 84pp. doi: /j.efsa WALES, A. & DAVIES, R. H., (2013). Environmental aspects of Salmonella. In: Salmonella in domestic animals, (Ed. 2), Eds. Barrow, P. A., & Methner, U, CABI ANON. (2013). How pork safety is about to impact the security of your business. An evening meeting showcasing new information all pig businesses must know. Four evening presentations to UK pig producers, organised by AHVLA and BPEX. ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO 17025, GLP, ISO Is your laboratory accredited by an international accreditation body? 8 OIE Reference Laboratory Reports Activities, 2013

9 Test for which your laboratory is accredited Various Salmonella surveillance and diagnostic tests Various serological and AMR tests Various research methodologies Accreditation body UKAS UKAS Lloyds register 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) ToR: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? OIE Reference Laboratory Reports Activities,

10 Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Assess Laboratory capability to conduct isolation and identification of Salmonella species and quality system accreditation (AHVLA Vetqas PT scheme PT0087 Salmonella in Animal Feed) Participant 16 UK Assess Laboratory capability to conduct isolation and identification of Salmonella species and quality system accreditation (AHVLA Vetqas PT scheme PT0088 Salmonella in Poultry) Participant 134 UK, Canada Assess Laboratory capability to conduct isolation and identification of Salmonella species and quality system accreditation (AHVLA Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order - run for Defra for UK labs only) Participant 25 UK 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories Validation of monophasic S. Typhimurium PCRs Validation of monophasic S. Typhimurium PCRs Italy Molecular serotyping study Evaluation of molecular methods for serotyping Salmonella Netherlands, Canada ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point OIE Reference Laboratory Reports Activities, 2013

11 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Assess Laboratory capability to conduct isolation and identification of Salmonella species and quality system accreditation. Reports allow comparisons to be made (AHVLA Vetqas PT scheme PT0087 Salmonella in Animal Feed) 16 Asia and Pacific Middle East Assess Laboratory capability to conduct isolation and identification of Salmonella species and quality system accreditation. Reports allow comparisons to be made (AHVLA Vetqas PT scheme PT0088 Salmonella in Poultry) 134 Asia and Pacific Middle East Assess Laboratory capability to conduct isolation and identification of Salmonella species and quality system accreditation (AHVLA Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order - run for Defra for UK labs only) 26 Asia and Pacific Middle East Salmonella detection, serotyping and phagetyping EU RL ringtrials 27 Asia and Pacific Middle East EURL-Salmonella Interlaboratory Comparison Study on PFGE typing, pilot Asia and Pacific Middle East External quality assessment scheme for typing of Salmonella (Salmonella EQA5; PFGE and MLVA) 12 Asia and Pacific Middle East ToR: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Commissioned literature review UK/Italy Control of Salmonella in food producing animals Consultancy UK Control of S. Gallinarum outbreak in rthern Ireland 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,

12 This report was compiled by Doris Mueller-Doblies on behalf of Robert Davies. 12 OIE Reference Laboratory Reports Activities, 2013