CROATIAN VETERINARY INSTITUTE

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1 VPH dept. Laboratory for Food Microbiology Andrea Humski, Ph.D, DVM Marina Mikulić, DVM

2 about CVI... Established in Leading national diagnostic, research and scientific institution in the field of veterinary medicine -Ministry of Agriculture, Fishery and Rural Development (MPRRR) -Ministry of Science, Education and Sports (MZOS)

3 AREAS OF OPERATION 1. DIAGNOSTIC AND ANALYTICAL ACTIVITIES Preparedness, field and laboratory diagnostics, profilaxis, surveillance and monitoring, data analysis, planing and proposal of preventive actions, reference and scientific advisory functions in control of: Infectious diseases Zoonoses Food and feed safety Veterinary drugs and residues

4 2. SCIENTIFIC AND RESEARCH ACTIVITIES Performed within the framework of The National scientific programme Executed through the several scientific research projects financially supported by Ministry of science, education and sport

5 CVI laboratory network...employees total no. 238 PhD 34 Msc 13 Sci. advisor 7 Senior sci. associate - 10 Sci. associate 11

6 CP Križevci Vinkovci

7 CVI Zagreb I. VPH Dept. I.1. Laboratory for food microbiology I.2. Laboratory for residue control I.3. Laboratory for feed microbiology I.4. Laboratory for analytical chemistry I.5. Laboratory for veterinary medicinal products

8 LABORATORY FOR FOOD MICROBIOLOGY, Zagreb microbiological control of food: official control (import/export), on request (domestic production/market) monitoring programmes microbiological contamination of meat in slaughterhouses microbiology food safety of products- veterinary inspection on border posts research and scientific work

9 LABORATORY FOR FOOD MICROBIOLOGY ACCREDITATION CERTIFICATE Microbiological parameter/method 1 Salmonella spp. HRN EN ISO 6579:2003 / cor Salmonella spp. TECRA VIA Screening method AOAC Official Method ; Listeria monocytogenes Part 1.: Detection method HRN EN ISO : Listeria monocytogenes Part 2.: Enumeration method HRN EN ISO : Listeria spp. TECRA VIA Screening method AOAC Official Method ; Enumeration of microorganisms - CCT at 30 C HRN ISO 4833: Escherichia coli Part 2.:Colony count technique at 44 C HRN ISO : Escherichia coli Part 3.: MPN method ISO/TS : Escherichia coli O157 HRN EN ISO 16654: E. coli O157. TECRA VIA screening method AOAC RI, L.NO Enterobacteriaceae Part 1.: MPN method ISO : Enterobacteriaceae Part 2.: Colony count technique ISO : Staphylococcus aureus and other species Part 1.: Technique using Baird-Parker medium HRN EN ISO : Sulfite-reducing bacteria HRN ISO 15213: Clostridium perfringens - Colony count technique HRN EN ISO 7937: Campylobacter spp. Part 1. Detection method ISO : Campylobacter spp. Part 2. Enumeration method ISO : Campylobacter spp. TECRA VIA Screening method

10 External Quality Assesment/Proficiency Testing Health Protection Agency, London, UK (since 2005.) Parameter No. of tested samples Salmonella spp. 15 Listeria monocytogenes / E 8 Listeria monocytogenes / D 4 E. coli, MPN 9 E. coli / E 2 E. coli O157 3 ACC 11 Campylobacter spp. 6 Coag. + Staphylococcus / E 5 Staph. aureus enterotoxin 2 Cl. perfringens / E 3 Enterobacteriaceae / E 3

11 Present and future activities.

12 Zoonoses in humans Disease 2005 Cases Deaths Rate per 100,000 population Rank Salmonellosis Leptospirosis Q fever Toxoplasmosis Trichinellosis Disease 2006 Cases Deaths Rate per 100,000 population Rank Salmonellosis Trichinellosis Leptospirosis Toxoplasmosis Q fever Disease 2007 Cases Deaths Rate per 100,000 population Rank Salmonellosis Campylobacteriosis Leptospirosis Q fever Toxoplasmosis Disease 2008 Cases Deaths Rate per 100,000 population Rank Salmonellosis Campylobacteriosis Q fever Brucellosis Toxoplasmosis

13 Salmonellosis Campylobacteriosis

14 1. Monitoring programme - Ordinance on conduction of veterinary inspection and control of animal products in traffic across the border of the Republic of Croatia (O.G. 132/08) / Ordinance on the document for registration of veterinary health inspection of shipments of animal origin entering the Republic of Croatia (O.G.129/06 and 75/07) Annex II 2. Baseline study acc. to Commission Decision 2007/516/EC: Campylobacter and Salmonella prevalence in broiler flocks (carcasses + caecal content)

15 THANK YOU FOR ATTENTION

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