Trends en voorkomen van resistenties bij Salmonella, Campylobacter en E. coli geïsoleerd uit de voeding

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1 Trends en voorkomen van resistenties bij Salmonella, Campylobacter en E. coli geïsoleerd uit de voeding Cristina Garcia-Graells, Nadine Botteldoorn, Katelijne Dierick NRL AMR Food Pathogens - AMCRA 30/06/2017

2 Antibiotic resistance surveillance at FP Scientific Service Foodborne Pathogens N. Botteldoorn NRL antimicrobial resistance N. Botteldoorn & C.Garcia-Graells Samples/year Matrix Enumeration of ESBL E. coli (till 2015) Detection of β- lactamase producing E.coli ~ 100 samples ~ 300 samples poultry Veal, beef, pork, fish, poultry from 2015 E. coli strains for confirmation and identification of ESBL class

3 Antibiotic resistance surveillance at FP Isolates/year Salmonella Campylobacter ~ 450 ~ 700 Surveillance of antimicrobial resistance transmitted by food MIC (EUCAST) breakpoints ESBL phenotype and genotypes determined Accredited ISO Reported to EFSA ESBL E.coli ~ 300 Harmonisation of the methodologies at European level Antimicrobieel agens C. jejuni Tetracycline 1 Nalidixinezuur 16 Ciprofloxacine 0.5 Erythromycine 4 Gentamicine 2 Streptomycine 4 Interpretatie breekpunten voor R>(mg/l) Antimicrobieel agens ECOFF (R> mg/l)* E. coli ECOFF (R> mg/l)* Salmonella Ampicilline 8 8 Cefotaxime Ceftazidime Meropenem Nalidixinezuur Ciprofloxacine Tetracycline 8 8 Colistine 2 2 Gentamicine 2 2 Trimethoprim 2 2 Sulfamethoxazol ** Chlooramfenicol Azitromycine 16** 16** Tigecycline 1 1 OD-Communicable and infectious diseases

4 % R Campylobacter 2016 Number of strains received N=670 ID with Maldi-Tof Matrices: broiler carcasses (54%) Spent hens (19.18%), Poultry meat (22.11%) Results of the MIC cj (n=370) Campylobacter jejuni CIP NAL TET ERY STR GEN Antimicrobiens Trend : Co-Resistance CipNalTet stay very high C.jejuni specific clone Level of resistance found for the different antibiotics tested in C. jejuni isolated for poultry meat (n=370)

5 Study of the resistance Human-animal-food : one health ve_pages/amr_report_2015

6 %R Salmonella_2016 (I) Salmonella Food o Matrices: porc carcasses (45% d isolats), carcasses of spent hens (22%), meat preparation (9.25%), other (fish, crustacés) o MIC réalisés n= 227 AB Sulfaméthoxazole 256 cut-off (R>) n % Ampicilline Tétracycline Ciprofloxacine Triméthoprime Acide Nalidixique Tigécycline Chloramphenicol Azithromycine Colistine Céfotaxime Gentamicine Ceftazidime Méropénème High Moderate ,21 33,48 29, ,47 20,70 18, ,93 7,49 4,85 3,52 2,20 1,32 0,88 0,00 SMX AMP TET CIP TMP NAL TGC CHL AZI COL FOT GEN TAZ MERO Antimicrobiens Low R aux FQ (n=51): + 3 th gen. cephalosporins (4/51, 3%) + Colistine (2/51, 3.9%) + Tigécycline (7/51, 13.72%) 1 isolate R at all antibiotics tested except méropénème (IEC 016, Vietnam) AmpAziFotTazChlCipColGenNalSmxTetTigTmp % R to the antibiotics tested in Salmonella spp. (n=227)

7 %R Salmonella EU-AMR (broiler carcasses) Very high High modérate low 56,82 56,82 54,55 26,70 22,73 17,05 8,52 3,41 2,84 2,84 2,27 1,70 1,70 1,14 SMX CIP NAL TMP AMP TET AZI FOT COL TGC TAZ CHL MERO GEN Antimicrobiens Resistance par serovar

8 Salmonella EU-AMR (broiler carcasses) Comparison between Multi resistance n % Pas de résistance R à R à R à R à R à R à Total % MDR Serovar related : more S. Infantis isolated

9 Salmonella ESBL Salmonella isolates detected with R to cefotaxime or ceftazidime Those strains were confirmed by running the second panel and classified according the EFSA criteria E U A M R F O O D Matrix/serovar/phenotype n Broiler carcasses 7 Auto-Agglutinable 2 AmpC 1 ESBL 1 Infantis 2 AmpC 1 ESBL+AmpC 1 Paratyphi B Var L (+) Tartrate + 3 AmpC 1 ESBL+AmpC 2 Porc carcasses 1 Subspi I autre phénotype Frog legs 1 Saintpaul ESBL+AmpC Poultry meat 2 Paratyphi B Var L (+) Tartrate + ESBL ESBL+AmpC Total 11 The different ESBL phenotypes detected in 2016

10 Indicator organisms : β-lactamase producing E. coli ESBL Enumeration of E. coli ESBL on poultry carcasses

11 Results E. coli ESBL 2016 From now on a detection is performed in 25g of the food sample Nr of food Samples ESBL + (25 g) % Poultry Bovine Porc Fish Crustacea ESBL phenotype found in poultry

12 β-lactamase producing E. coli : trend in poultry 2016 vs n= n=188

13 Thanks for your attention