ZOONOSES MONITORING. Belgium IN 2016 TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS

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1 ZOONOSES MONITORING Belgium TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS including information on foodborne outbreak, antimicrobial reitance in zoonotic and indicator bacteria and ome pathogenic microbiological agent IN 6 Belgium - 6

2 Belgium - 6 Report on trend and ource of zoonoe PREFACE Thi report i ubmitted to the European Commiion in accordance with Article 9 of Council Directive 3/99/ EC*. The information ha alo been forwarded to the European Food Safety Authority (EFSA). The report contain information on trend and ource of zoonoe and zoonotic agent in Belgium during the year 6. The information cover the occurrence of thee dieae and agent in animal, foodtuff and in ome cae alo in feedingtuff. In addition the report include data on antimicrobial reitance in ome zoonotic agent and indicator bacteria a well a information on epidemiological invetigation of foodborne outbreak. Complementary data on uceptible animal population in the country i alo given. The information given cover both zoonoe that are important for the public health in the whole European Union a well a zoonoe, which are relevant on the bai of the national epidemiological ituation. The report decribe the monitoring ytem in place and the prevention and control trategie applied in the country. For ome zoonoe thi monitoring i baed on legal requirement laid down by the European Union legilation, while for the other zoonoe national approache are applied. The report preent the reult of the examination carried out in the reporting year. A national evaluation of the epidemiological ituation, with pecial reference to trend and ource of zoonotic infection, i given. Whenever poible, the relevance of finding in foodtuff and animal to zoonoe cae in human i evaluated. The information covered by thi report i ued in the annual European Union Summary Report on zoonoe and antimicrobial reitance that are publihed each year by EFSA. * Directive 3/ 99/ EC of the European Parliament and of the Council of December 3 on the monitoring of zoonoe and zoonotic agent, amending Deciion 9/ / EEC and repealing Council Directive 9/ 7/ EEC, OJ L 35, 7..3, p. 3 Belgium - 6

3 Lit of Content TEXTFORMS ANIMAL POPULATIONS. Population.. Information on uceptible animal population DISEASE STATUS. TUBERCULOSIS, MYCOBACTERIAL DISEASES.. General evaluation of the national ituation... Mycobacterium - general evaluation.. Mycobacterium in animal... Mycobacterium tuberculoi complex (MTC) in animal - Deer - farmed - animal ample... Mycobacterium tuberculoi complex (MTC) in animal - Cattle (bovine animal) - animal ample. BRUCELLOSIS.. Brucella in animal... B. ui in animal - All animal - animal ample 7... B. ui in animal - Pig - animal ample B. abortu in animal - Cattle (bovine animal) - animal ample... B. meliteni in animal - Goat - animal ample...5 B. meliteni in animal - Sheep - animal ample 3 INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS 3 3. SALMONELLOSIS in foodtuff in food - All foodtuff - Unpecified - food ample - Surveillance - Official ampling - Objective ampling in animal in animal - Gallu gallu (fowl) - broiler - Farm - environmental ample - boot wab - Control and eradication programme - Official and indutry ampling - Cenu in animal - Gallu gallu (fowl) - laying hen - Farm - environmental ample - boot wab and dut - Control and eradication programme - Official and indutry ampling - Cenu in animal - Gallu gallu (fowl) - breeding flock, unpecified - Farm - environmental ample - boot wab - Control and eradication programme - Official and indutry ampling - Cenu 3... in Turkey - breeding flock and meat production flock 3 3. CPYLOBACTERIOSIS 3.. General evaluation of the national ituation 3... Thermophilic Campylobacter pp., unpecified - general evaluation 3.. Campylobacter in foodtuff Thermophilic Campylobacter pp., unpecified in food - Meat from broiler (Gallu gallu) - food ample Thermophilic Campylobacter pp., unpecified in food - All foodtuff - food ample Campylobacter in animal Thermophilic Campylobacter pp., unpecified in animal - Gallu gallu (fowl) - animal ample - caecum LISTERIOSIS 3.3. General evaluation of the national ituation Literia - general evaluation 3.3. Literia in foodtuff L. monocytogene in food - All foodtuff - Unpecified - food ample - Surveillance - Official ampling - Objective ampling 3. YERSINIOSIS General evaluation of the national ituation Yerinia - general evaluation TRICHINELLOSIS 3.5. General evaluation of the national ituation Trichinella - general evaluation Trichinella in animal Trichinella in animal - Soliped, dometic - hore - animal ample Trichinella in animal - Pig - animal ample ECHINOCOCCOSIS General evaluation of the national ituation Echinococcu - general evaluation RABIES General evaluation of the national ituation Lyaviru (rabie) - general evaluation Lyaviru (rabie) in animal Lyaviru (rabie) in animal - All animal - animal ample STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) INFECTION Staphylococcu in animal S. aureu, meticillin reitant (MRSA) in animal - Pig - Farm - animal ample - naal wab - Monitoring - Official ampling - Objective ampling Q-FEVER Coxiella (Q-fever) in animal Coxiella (Q-fever) in animal - Cattle (bovine animal) - adult cattle over year - Farm - animal ample Coxiella (Q-fever) in animal - Sheep and goat - Farm - animal ample CYSTICERCOSIS, TAENIOSIS 3.. General evaluation of the national ituation 3... Cyticerci - general evaluation 3. SARCOCYSTOSIS 3.. General evaluation of the national ituation 3... Sarcocyti - general evaluation 3. TOXOPLASMA 3.. General evaluation of the national ituation 3... Toxoplama - general evaluation 3.3 VTEC Echerichia coli in foodtuff Verotoxigenic E. coli (VTEC) in food - All foodtuff - food ample 3 ANTIROBIAL RESISTANCE INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS 5. SALMONELLOSIS 5.. in foodtuff 5... Antimicrobial reitance in Meat from broiler (Gallu gallu) 5... Antimicrobial reitance in Other food Antimicrobial reitance in pp., unpecified Meat from bovine animal 7... Antimicrobial reitance in pp., unpecified Meat from broiler (Gallu gallu) Antimicrobial reitance in pp., unpecified Meat from pig.. in animal... Antimicrobial reitance in pp., unpecified Gallu gallu (fowl). CPYLOBACTERIOSIS 5.. Campylobacter in foodtuff 5... Antimicrobial reitance in C. jejuni Meat from poultry, unpecified 5.. Campylobacter in animal 5... Antimicrobial reitance in Thermophilic Campylobacter pp., unpecified Gallu gallu (fowl) 5.3 ESCHERICHIA COLI, NON-PATHOGENIC 5.3. Echerichia coli, non-pathogenic in animal Antimicrobial reitance in E.coli, non-pathogenic, unpecified Cattle (bovine animal) Antimicrobial reitance in E.coli, non-pathogenic, unpecified Gallu gallu (fowl) Antimicrobial reitance in E.coli, non-pathogenic, unpecified Pig 55. STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) INFECTION 56.. Staphylococcu in animal Antimicrobial reitance in S. aureu, meticillin reitant (MRSA) Pig 56 5 INFORMATION ON SPECIFIC ROBIOLOGICAL AGENTS 5 5. CRONOBACTER Cronobacter in foodtuff Cronobacter in food - Infant formula - food ample 5 5. HISTINE Hitamine in foodtuff Hitamine in food - Fih - Fihery product from fih pecie aociated with a high amount of hitidine - not enzyme maturated - food ample

4 6 FOODBORNE OUTBREAKS Outbreak Foodborne outbreak 6 ANIMAL POPULATION TABLES 6 DISEASE STATUS TABLES FOR BRUCELLA 63 Bovine brucelloi in countrie and region that do not receive Community co-financing for eradication programme 63 Ovine or Caprine brucelloi in countrie and region that do not receive Community co-financing for eradication programme 6 DISEASE STATUS TABLES FOR MYCOBACTERIUM 65 Bovine tuberculoi in countrie and region that do not receive Community co-financing for eradication programme 65 PREVALENCE TABLES 66 CPYLOBACTER 66 animal 66 food COXIELLA animal 69 CRONOBACTER 7 food 7 CYSTICERCUS 7 animal 7 ECHINOCOCCUS 7 animal 7 ESCHERICHIA COLI 73 food FLAVIVIRUS animal 75 HISTINE 76 food 76 LISTERIA 77 food 77 LYSSAVIRUS 6 animal 6 SALMONELLA 7 animal 7 food 9 feed 99 STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) 6 animal 6 TRICHINELLA 7 animal 7 YERSINIA food FOODBORNE OUTBREAKS TABLES 9 R TABLES FOR CPYLOBACTER 6 Campylobacter jejuni 6 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 6 6 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 7 7 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 9 9 Meat from broiler (Gallu gallu) - freh - Proceing plant - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Monitoring - Official ampling - OTHER R MON 3 3 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 5 5 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - active - Official ampling - R MON 6 6 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - Official ampling - OTHER R MON 7 7 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 9 9 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 3 3 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 3 3 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 3 3 R TABLES FOR SALMONELLA 33,,[5],:i:- 33 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Meat from other animal pecie or not pecified - Retail - Monitoring - Official ampling - OTHER R MON Meat, mixed meat - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from goat - freh - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 3 3 Other food - Retail - Monitoring - Official ampling - OTHER R MON Meat from pig - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON,5,:i:- Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 6,7:z9 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 3 3 Agona Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Compound feedingtuff, not pecified - final product - Farm - Control and eradication programme - Official ampling - OTHER R MON 5 5 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON

5 6 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Bardo Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Brandenburg 9 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 9 9 Meat from pig - Retail - Monitoring - Official ampling - OTHER R MON 5 Frog leg - Retail - Monitoring - Official ampling - OTHER R MON 5 5 Bredeney Meat, mixed meat - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON Cheter 5 Nut and nut product - Retail - Monitoring - Official ampling - OTHER R MON 5 Colorado 5 55 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Derby 56 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 5 5 Meat from pig - Retail - Monitoring - Official ampling - OTHER R MON enterica, ubpecie enterica 6 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON pnl 6 6 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 6 6 Complementary feedingtuff - Retail - Monitoring - Official ampling - OTHER R MON 6 6 Complementary feedingtuff - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 6 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from goat - freh - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON enterica, unpecified O:9 6 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON 6 6 Enteritidi 69 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 69 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON 69 7 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 7 7 Meat from poultry, unpecified - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Egg product - Retail - Monitoring - Official ampling - OTHER R MON 7 7 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON 7 7 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Gaminara 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 3 3 Give Other food - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON 5 5 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 6 6 Idikan 7 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 7 7 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON 9 9 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON 9 9 Indiana 9 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 9 Infanti Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 9 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON

6 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 9 9 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from poultry, unpecified - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat, mixed meat - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from poultry, unpecified - carcae - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON 5 5 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON pnl 6 6 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON 7 7 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 9 9 Javiana Frog leg - Retail - Monitoring - Official ampling - OTHER R MON Kaenyi Other food - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Kedougou Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Kentucky 3 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Other food - Retail - Monitoring - Official ampling - OTHER R MON Kottbu 5 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 5 5 Livingtone 6 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 6 6 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 7 7 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Llandoff Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON London Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Mbandaka 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON 5 5 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON 6 6 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 7 7 Milwaukee Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON Moero 9 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 9 9 Newport 3 Compound feedingtuff, not pecified - final product - Farm - Control and eradication programme - Official ampling - OTHER R MON 3 3 Ohio 3 Meat from pig - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Panama 3 Frog leg - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Paratyphi B 33 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON pnl 3 3 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON

7 Meat, mixed meat - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON pnl 3 3 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON Rien Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 5 5 Other feed material - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 6 6 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 7 7 Saintpaul Frog leg - Retail - Monitoring - Official ampling - OTHER R MON pnl Frog leg - Retail - Monitoring - Official ampling - OTHER R MON 9 9 Senftenberg 5 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 5 5 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON 5 5 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON 5 5 pp., unpecified 53 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 5 5 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON Meat from poultry, unpecified - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Compound feedingtuff, not pecified - final product - Farm - Control and eradication programme - Official ampling - OTHER R MON Meat from goat - freh - Slaughterhoue - Monitoring - Official ampling - OTHER R MON 5 5 Cheee, made from unpecified milk or other animal milk - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON pnl 6 6 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON 6 6 Stanley 6 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 6 6 Teddington 63 Other proceed food product and prepared dihe - vegetable baed dihe - Retail - Monitoring - Official ampling - OTHER R MON Tenneee 6 Other feed material - Retail - Monitoring - Official ampling - OTHER R MON 6 6 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official and indutry ampling - R MON Typhimurium 66 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 6 6 Gallu gallu (fowl) - laying hen - Farm - Monitoring - active - Indutry ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official and indutry ampling - R MON 7 7 Compound feedingtuff, not pecified - final product - Farm - Control and eradication programme - Official ampling - OTHER R MON 7 7 Meat, mixed meat - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 7 7 Crutacean - Retail - Monitoring - Official ampling - OTHER R MON Meat from pig - Retail - Monitoring - Official ampling - OTHER R MON 7 7 Meat from bovine animal and pig - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - EFSA pecification - Official ampling - R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - OTHER R MON Wandworth 7 Frog leg - Retail - Monitoring - Official ampling - OTHER R MON 7 Weltevreden Other food - Retail - Monitoring - Official ampling - OTHER R MON 79 R TABLES FOR ESCHERICHIA COLI Echerichia coli, non-pathogenic, unpecified Meat from broiler (Gallu gallu) - carcae - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from broiler (Gallu gallu) - carcae - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - EFSA pecification - Official ampling - ESBL MON pnl Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - EFSA pecification - Official ampling - ESBL MON 3 3 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - EFSA pecification - Official ampling - ESBL MON pnl Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - EFSA pecification - Official ampling - ESBL MON 6 6 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - EFSA pecification - Official ampling - ESBL MON pnl Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - EFSA pecification - Official ampling - ESBL MON 9 9 Meat from turkey - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl 9 9 Meat from turkey - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 9 9 Meat from bovine animal - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl

8 9 Meat from bovine animal - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl 9 9 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Cattle (bovine animal) - calve (under year) - Slaughterhoue - Monitoring - active - Official ampling - R MON Cattle (bovine animal) - calve (under year) - Slaughterhoue - Monitoring - active - Official ampling - ESBL MON pnl 9 9 Cattle (bovine animal) - calve (under year) - Slaughterhoue - Monitoring - active - Official ampling - ESBL MON 3 3 Cattle (bovine animal) - meat production animal - Farm - Monitoring - active - Official ampling - OTHER R MON pnl 3 3 Cattle (bovine animal) - meat production animal - Farm - Monitoring - active - Official ampling - OTHER R MON Pig - fattening pig - Slaughterhoue - Monitoring - active - Official ampling - R MON Pig - fattening pig - Slaughterhoue - Monitoring - active - Official ampling - ESBL MON pnl 37 Pig - fattening pig - Slaughterhoue - Monitoring - active - Official ampling - ESBL MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl 3 3 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - minced meat - Retail - Monitoring - Official ampling - OTHER R MON pnl 3 3 Meat from broiler (Gallu gallu) - minced meat - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - minced meat - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from broiler (Gallu gallu) - minced meat - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - active - Official ampling - R MON pnl 3 3 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - active - Official ampling - R MON 3 3 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - active - Official ampling - ESBL MON pnl 3 3 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - active - Official ampling - ESBL MON Meat from other animal pecie or not pecified - Retail - Monitoring - Official ampling - OTHER R MON pnl 3 Meat from other animal pecie or not pecified - Retail - Monitoring - Official ampling - OTHER R MON Crutacean - unpecified - Retail - Monitoring - Official ampling - OTHER R MON pnl Crutacean - unpecified - Retail - Monitoring - Official ampling - OTHER R MON Meat from pig - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from pig - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from pig - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from pig - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from pig - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from pig - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from broiler (Gallu gallu) - Retail - Monitoring - Official ampling - OTHER R MON 3 3 Meat from bovine animal - minced meat - Retail - Monitoring - Official ampling - OTHER R MON pnl 3 3 Meat from bovine animal - minced meat - Retail - Monitoring - Official ampling - OTHER R MON Meat from bovine animal - minced meat - Retail - Monitoring - Official ampling - OTHER R MON pnl 3 3 Meat from bovine animal - minced meat - Retail - Monitoring - Official ampling - OTHER R MON Meat from bovine animal - minced meat - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from bovine animal - minced meat - Retail - Monitoring - Official ampling - OTHER R MON Fih - raw - Retail - Monitoring - Official ampling - OTHER R MON pnl 3 3 Fih - raw - Retail - Monitoring - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON pnl 35 Meat from broiler (Gallu gallu) - meat preparation - Retail - Monitoring - Official ampling - OTHER R MON Meat from pig - meat product - Retail - Monitoring - Official ampling - OTHER R MON pnl Meat from pig - meat product - Retail - Monitoring - Official ampling - OTHER R MON 35 OTHER R TABLES Methicillin reitant Staphylococcu aureu (MRSA) Pig - fattening pig - Farm - Monitoring - active - Official ampling - OTHER R MON Pig - breeding animal - Farm - Monitoring - active - Official ampling - OTHER R MON ESBL LATEST TRANSMISSIONS

9 ANIMAL POPULATIONS The relevance of the finding on zoonoe and zoonotic agent ha to be related to the ize and nature of the animal population in the country. Population.. Information on uceptible animal population Source of information SANITEL and BELTRACE databae of the Federal Agency for the Safety of the Food Chain. Date the figure relate to and the content of the figure Number of animal = number of animal at a certain time point of the year. Number of laughtered animal = total number of laughtered animal during the year. Definition ued for different type of animal, herd, flock and holding a well a the type covered by the information Holding: any etablihment, contruction or, in the cae of an open-air farm, any place in which animal are held, kept or handled. The location of the holding i baed on the addre and the coordinate of the geographical entity. A geographical entity i a unit of one building or a complex of building included ground and territorie where an animal pecie i or could be hold. Herd: an animal or group of animal kept on a holding a an epidemiological unit; if more than one herd i kept on a holding, each of thee herd hall form a ditinct unit and hall have the ame health tatu. National evaluation of the number of uceptible population and trend in thee figure Over the lat year, there' a continuou decreae in total number of holding of animal pecie. The total number of bovine animal remain unchanged what mean that the mean total number of animal per holding i increaing. The total number of holding and animal of wine, are decreaing over the lat year. Geographical ditribution and ize ditribution of the herd, flock and holding Belgium can be geographically divided into two region: the Flemih region ituated in the north and the Walloon region ituated in the outh of the country. There' a very dene animal population of bovine, wine and poultry in the Flemih region. The Walloon region i important for hi cattle breeding holding of the Belgian Blue White race. The number of porcine and poultry holding in the Walloon region i rather limited. Belgium - 6 3

10 DISEASE STATUS. TUBERCULOSIS, MYCOBACTERIAL DISEASES.. General evaluation of the national ituation... Mycobacterium - general evaluation Hitory of the dieae and/or infection in the country Zoonotic tuberculoi (Mycobacterium bovi). Bovine tuberculoi in human caued by M. bovi i clinically inditinguihable from human tuberculoi caued by M. tuberculoi. In the pat, the mot important way of tranmiion of M. bovi to human wa the conumption of raw milk or raw milk product from infected cattle. Indutrial heating during production method or pateurization of raw milk did top thi way of tranmiion to human. Nowaday tuberculoi in human caued by M. bovi i rare. In region were M. bovi infection in cattle are largely eliminated, only few reidual cae occur among elderly peron a a reult of the reactivation of dormant M. bovi within old leion. Alo among migrant from high-prevalence countrie or region, infection with M. bovi are diagnoed. Agricultural worker may acquire infection by M. bovi by inhaling cough aerool from infected cattle and may ubequently develop typical pulmonary or genito-urinary tuberculoi. Cervical lymphadenopathy, intetinal leion, chronic kin tuberculoi (lupu vulgari) and other non-pulmonary form are alo particularly common a clinical ymptom. Recent action taken to control the zoonoe The urveillance program of tuberculoi i baed on Directive 6/3/EEC, which i implemented and adapted in National legilation ince 963 and lat modified by the Royal Decree of 7 October. The control implie kin teting of animal at the occaion of trade and intenive teting of infected and contact farm in conequence of a confirmation of a bovine TB upiciou cae (tracing-on and tracing-back of all contact animal and contact herd). Sytematic ante- and pot-mortem examination are performed at all laughterhoue. The Federal Agency for the Safety of the Food chain i informed about any doubtful or poitive reult of the kin tet of bovine and may decide to re-examine (additional tet e.g. comparative tuberculin tet, interferon-gamma tet) the animal or to kill them for additional analyi (tet and laughter trategy). In cae a "TB upiciou" leion i detected, a tiue ample i ent to the National Reference Laboratory for analyi. Conequently, if Mycobacterium bovi upicion i confirmed by a poitive culture or PCR, all animal in the herd of origin are kin teted and an epidemiological invetigation i realized. The total herd i conidered a the 'epidemiological unit'. Iolation of M. bovi and biochemical teting i excluively performed in the National Reference Laboratory where alo IFN-gamma, PCR and molecular typing by mean of RFLP, poligotyping or more recently MIRU-VNTR are done to upport the epidemiological invetigation and to eventually prove the link between different cae. Suggetion to the European Union for the action to be taken In cae a holding i infected and if by epidemiological invetigation and tracing-back, animal were found to have been exported to another country, the Chief Veterinary Officer of the country of detination ha to be informed about the outbreak in the country of origin. Thi alert can help to rapidly detect an infection in the concerned holding of detination abroad. Monitoring of the type of train circulating in each country could contribute to the undertanding of the temporal-patial pread of ome pecific train between different countrie and could poibly bear ome epidemiological link between different outbreak. More attention hould be given to intracommunity trade in animal enitive to bovine tuberculoi (e.g. camelid), epecially if thoe animal have tayed for a time in an endemic region of tuberculoi. Attention hould alo be given to early detect a poible incurrence of infected wildlife a deer, wildboar and badger from neighbouring countrie... Mycobacterium in animal... Mycobacterium tuberculoi complex (MTC) in animal - Deer - farmed - animal ample Monitoring ytem Sampling trategy Belgium - 6

11 Sampling in cae of upiciou TB leion during pot-mortem examination of "wild" and "farmed" deer at laughterhoue/ at game handling etablihment. Frequency of the ampling Depend on the number of hunted/laughtered animal and the detection of upiciou leion at pot-mortem examination. Type of pecimen taken Supiciou leion of lung, lymph node,... at laughterhoue or game handling etablihment. Method of ampling (decription of ampling technique) TB upiciou tiue: lymph node, lung,... Cae definition An animal i poitive if Mycobacterium bovi i iolated by culture or confirmed by laboratory analyi. Diagnotic/analytical method ued - Ziehl-Neelen coloration - Culture for iolation - Interferon-gamma - PCR on leion / organ - PCR on culture Control program/mechanim The control program/trategie in place Monitoring i done by:- ytematic pot-mortem examination at the laughterhoue/game handling etablihment- pot-mortem examination at autopy of hunted or killed "wild" deer by accident in the Univerity Center of Liège, Veterinary Medicine Faculty. In cae of upected TB leion, tiue ample are ent to the National Reference Laboratory for additional analyi to confirm the upicion. Recent action taken to control the zoonoe Surveillance program in wildlife. National evaluation of the recent ituation, the trend and ource of infection No Mycobacterium bovi wa detected in "wild/hunted" or "farmed" deer for the reporting year Mycobacterium tuberculoi complex (MTC) in animal - Cattle (bovine animal) - animal ample Statu a officially free of bovine tuberculoi during the reporting year The entire country free Belgium i officially free of bovine tuberculoi ince the 5th of June 3 (Commiion Deciion 3/67/EC) Free region All region are officially free of bovine tuberculoi. Belgium - 6 5

12 Monitoring ytem Sampling trategy Surveillance ytem.the control of tuberculoi i baed on Council Directive 6/3/EEC, which i implemented and adapted in National legilation ince 963 and wa lat modified by the Royal Decree of 7 October. The urveillance program implie:- kin teting of all animal at purchae by the veterinarian reponible for the epidemiological anitary ituation of the holding (contract between farmer and veterinarian); - in cae of a upected/infected bovine() on a holding kin teting of all animal of the holding; - kin teting of all 'contact' animal and herd (tracing-on and tracing-back);- ytematic ante- and pot-mortem examination of all laughtered bovine, tranmiion to the National Reference Laboratory of all "TB upiciou" leion for further analyi. Iolation of M. bovi and typing i performed at the National Reference Laboratory CODA-CERVA. Alo IFN-gamma, PCR and molecular typing by mean of RFLP, poligotyping and more recently MIRU-VNTR are realied at the NRL. Frequency of the ampling Frequency of teting depend on:- the introduction of new animal into a herd (mandatory examination at purchae) - the reult of tuberculin teting- the detection of upected bovine- the detection of infected bovine- the epidemiological invetigation related to upected or infected animal or herd (tracing-on and tracing-back)- the follow-up teting of infected and/or eradicated herd during 5 year after partial or total tamping-out. Thi follow-up teting can be reduced to year, year 3 and year 5 if epidemiological invetigation indicate a low rik of infection- an 'at random election' of holding in the category of holding with an important number of purchaed animal per year: tuberculination of all purchaed bovine over the lat year. Type of pecimen taken Organ/tiue: leion, lymph node, lung, liver, kidney, pleen,...blood Method of ampling (decription of ampling technique) Tuberculin kin teting: (bovine tuberculin) or comparative (bovine/avian tuberculin) teting. Blood ampling: interferon-gamma tet Laboratory examination of all upiciou leion by culture: iolation and identification or analyi by PCR. Organ: lymph node, lung, liver, kidney,... Cae definition - A 'bovine' i defined a infected with bovine tuberculoi if the animal i poitive by kin teting or if Mycobacterium bovi i iolated by culture or confirmed by laboratory analyi (PCR). - A 'holding' i defined a infected if Mycobacterium bovi wa iolated by culture from an animal of the holding. Diagnotic/analytical method ued - Simple kin tet with bovine tuberculin- Comparative kin tet with bovine and avian tuberculin- Ziehl-Neelen coloration- Culture for iolation- Interferon-gamma aay- PCR on leion / organ- PCR on culture - RFLP typing- Spoligotyping- MIRU-VNTR Vaccination policy Vaccination i prohibited by Royal Decree of 7 October. Control program/mechanim The control program/trategie in place National urveillance program by the Competent Authority (FASFC) on a mandatory legal bae. Recent action taken to control the zoonoe Belgium - 6 6

13 Draw pecial attention and focu on the pot-mortem examination of laughtered animal; Tranmiion for further analyi of any leion that could be 'upected' of tuberculoi to the National Reference Laboratory; Culture of M. bovi, biochemical teting, PCR are performed on thee 'upiciou' leion; Molecular typing by mean of RFLP, Spogilotyping and more recently MIRU-VNTR are realied on all to upport the epidemiological invetigation and to eventually prove the link between different cae or outbreak. Suggetion to the European Union for the action to be taken In cae of export of bovine, inform the Chief Veterinary Officer of the Member tate of detination if tuberculoi ha been detected in a holding of the Member State of origin after the date of export. Thi information can reult in an early detection or can avoid a poible further contamination in the Member State of detination. Meaure in cae of the poitive finding or cae If M. bovi i upected, all animal in the herd of origin are kin teted, the herd i conidered a the epidemiological unit. A complete epidemiological invetigation i performed. After tracing-back and tracing-on all animal of 'contact' holding are examined by kin teting. If any doubtful or poitive reult of the kin tet i detected, the FASFC may decide to re-examine the reactor animal (additional tet e.g. comparative kin teting with avian and bovine tuberculin and/or Interferon-gamma teting) or to a direct mandatory laughter of the reactor (tet laughter) for additional analyi. In cae a upiciou leion i detected at pot-mortem examination, a ample i ent to the National reference laboratory for analyi. If in conequence Mycobacterium bovi i iolated, all kin tet poitive animal during ucceive teting are mandatory laughtered. If many bovine are reacting poitive to kin teting, the FASFC can decide that all animal of the holding mut be mandatory laughtered (total tamping-out). In mot breakdown a anitation plan i etablihed taking into account the epidemiological ituation. In cae of partial tampingout, only anitation plan may be realied. After tamping-out, new retocked animal are teted three time during a 5 year period by annual kin teting to prove the TB free tatu of the holding. Notification ytem in place Animal Health Law of March 97 Chapter III and Royal Decree of 3 February (lit of all notifiable animal dieae). Reult of the invetigation In 6, outbreak of bovine tuberculoi were detected. On the firt outbreak (6-) a general tamping-out wa applied and contact herd had to be followed-up by tuberculination. Hereby one ecundary outbreak wa detected with a clear epidemiological link to the primary cae. On the econd outbreak (6-) alo a general tamping-out wa organized. Due to thi econd outbreak 9 contact herd had to be followed-up by tuberculination. Of outbreak 6- and 6-, repectively and 7 where obtained by bacteriological examination. National evaluation of the recent ituation, the trend and ource of infection Number of infected herd ince = : / : 3 / : 3 / 3 : 7 / : / 5 : 5 / 6 : / 7 : 5 / : / 9 : / : / : / : / 3: 9 / : / 5: 3 / 6:. BRUCELLOSIS.. Brucella in animal... B. ui in animal - All animal - animal ample Monitoring ytem Cae definition An animal i poitive if Brucella ui i iolated by culture or typed by additional laboratory method. Belgium - 6 7

14 ... B. ui in animal - Pig - animal ample Monitoring ytem Sampling trategy Serological creening for Brucella i done for breeding pig that are gathered (at a fair for example), at artificial inemination center and in animal intended for trade. The method ued are Roe Bengal tet (RBT), Slow Agglutination tet (SAT) according to Wright, Complement Fixation tet (CFT) and ELISA. Bacteriological examination for Brucella and Yerinia i done in cae of poitive erology. Regularly, fale poitive erological reaction are reported. Thee are due to a Yerinia enterocolitica O9 infection and are confirmed by Yerinia enterocolitica 9 iolation in the abence of Brucella pp. iolation. B. ui biovar may be iolated from wild boar (Su crofa). The infection eem to be endemic in wild boar in Belgium. B. ui biovar, circulating among wild boar, how only limited pathogenicity for human, if pathogenic at all.the dometic pig population i free of brucelloi (lat Brucella iolation in dometic pig in Belgium wa in 969). Method of ampling (decription of ampling technique) Blood ampling Tonil Spleen Diagnotic/analytical method ued Roe Bengal tet RBT / Complement fixation tet CFT / Indirect ELISA / Bacteriological examination...3 B. abortu in animal - Cattle (bovine animal) - animal ample Statu a officially free of bovine brucelloi during the reporting year The entire country free Belgium i officially free from bovine brucelloi ince the 5th of June 3 (Commiion Deciion 3/67/EC) Free region Belgium remained officially free of bovine brucelloi during thi reporting year. Additional information End a brucelloi breakdown herd wa detected after analyzing an abortion. The infected herd wa totally depopulated. In March, again a breakdown of brucelloi wa detected after analyi of an abortion. No epidemiological link could be found with the breakdown of. Tracing-back and an epidemiological inquiry lead to the detection of other econdary breakdown linked to the primary cae. All thee 5 brucelloi breakdown herd were infected with an identical Brucella abortu biovar 3. Another infected herd of brucelloi wa detected by analyi of tankmilk and an infection with Brucella ui biovar wa confirmed. Finally there wa a tampingout of all the animal of the infected herd. In 3 a breakdown herd wa detected a contact herd of the primary breakdown herd of. The breakdown herd of 3 wa already examined twice by erology in with negative reult. A third follow-up creening by erology indicated ome poitive reult. Thi poitive erology could be confirmed by culture after tet and laughter of the reactor. Finally 6 bovine were infected. There wa a tamping-out of all the animal of thi infected herd. In, bovine brucelloi wa not detected by a erological follow-up urveillance of contact herd of the brucelloi incident.in 5, no cae of brucella infection wa detected by a lat erological follow-up urveillance of contact herd during the winter campaign of the - 3 brucelloi incident.scientific advice 5-6 of the Scientific Committee of the FASFC on the re-emergence of bovine brucelloi in Belgium wa publihed in May 6. End of 6, in a holding of cattle, one bovine wa found infected with B. ui biovar by microbiological examination after mandatory tet laughter due to erological poitive reaction. Thi holding wa finally totally depopulated. Probably contact with wildboar could have been the origin of infection with thi opportunitic pathogen. Monitoring ytem Belgium - 6 Sampling trategy

15 Since Belgium i officially free of bovine brucelloi, the eradication program ha been changed in a urveillance program. An animal i legally upected of brucelloi in cae of a poitive ELISA. If, according to the epidemiology and the reult of the blood tet, an animal or herd i found to be at rik, a bacteriological invetigation alway take place. Hence, a brucelloi animal i defined a an animal in which Brucella abortu ha been iolated, and a cattle holding i conidered a an outbreak herd if one of the animal i poitive for brucelloi by bacteriological examination.in 9, a tudy wa realized to evaluate the current national urveillance program of bovine brucelloi. If a Member State ha maintained the officially free tatu of brucelloi for at leat 5 conecutive year, the exiting urveillance program can be re-evaluated and ome modification on the ampling deign are allowed on condition of further proof of freedom of dieae (Council Directive 6/3/EEC). The tudy alo clearly indicated that the bet approach i to tet bovine imported from officially free or nonofficially free Member State of Brucella pp., to tet animal at purchae in conequence of national trade a well a to analyze aborting animal in order to early detect infection. Alo the mandatory analyi for brucelloi at purchae of new animal changed into a voluntary approach. A new urveillance program ha been applied from the end of 9 on. In 6, urveillance wa focued on following rik categorie:- import of non officially free MS or Third Countrie at the moment of trade and follow-up teting during 3 conecutive year during the wintercreening (targeted election)- at random election of 5 bovine herd for erological invetigation of animal per herd divided in different age categorie: animal of 6- month of age, animal of - month of age and animal older then month. - number of analyi of bovine of national trade at purchae.- at random election of 5 bovine herd of all herd that did not declare any abortion during the pat year and did end ome lightweight bodie of newborn to the rendering plant. On thee herd a maximum of female animal are randomly elected for erological analyi of brucelloi. - abortion protocol: all abortion hould be notified and analyed for brucelloi.- a general creening of dairy herd by an ELISA of tankmilk wa realied in pring and autumn 6. Serological follow-up of a poitive tankmilk ample with tet laughter of one erological poitive reactor animal with microbiological examination proved the infection with Brucella ui biovar of that bovine. Frequency of the ampling - import of non officially free MS or Third Countrie at the moment of trade: all imported animal over month of age - import of non officially free MS or Third Countrie follow-up teting during wintercreening for 3 conecutive year of all imported animal over month of age- at random election of 5 bovine herd: at random election of maximum female animal in different age categoriebovine of national trade at purchae: at random election, limited number of analyi - at random election of 5 bovine herd where no abortion wa declared/analyzed during the lat year, at random election of female animal over month of age (goal i to timulate the notification of abortion)- abortion protocol: examination of abortion for brucelloi and ome other dieae which can induce an abortion in bovine animal (IBR, BVD, Neoplamoe,...). Type of pecimen taken Blood ample, Tankmilk, individual milk ample Method of ampling (decription of ampling technique) Blood ampling by veterinary practioner Tankmilk ample taken for Milk Quality Aurance Aociation at the moment of the collection of the milk by the milk factory Cae definition An animal i defined a infected if Brucella pp. ha been iolated by culture and identified a Brucelloi. A herd i defined a infected if one of it animal i poitive by bacteriological examination for Brucelloi. Diagnotic/analytical method ued - Micro agglutination tet - ELISA on blood or tank milk - Complement Fixation Tet- Roe Bengale Tet- PCR- Stamp/Ziehl Neelen coloration- Culture Vaccination policy Vaccination i prohibited in Belgium ince 99. Control program/mechanim The control program/trategie in place National mandatory urveillance program organized by the FASFC. Belgium - 6 9