ZOONOSES MONITORING. Czech Republic IN 2016 TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS

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1 ZOONOSES MONITORING Czech Republic TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS including information on foodborne outbreak, antimicrobial reitance in zoonotic and indicator bacteria and ome pathogenic microbiological ag IN 6 Czech Republic - 6

2 Czech Republic - 6 Report on trend and ource of zoonoe PREFACE Thi report i ubmitted to the European Commiion in accordance with Article 9 of Council Directive 3/99/ EC*. The information ha alo been forwarded to the European Food Safety Authority (EFSA). The report contain information on trend and ource of zoonoe and zoonotic ag in Czech Republic during the year 6. The information cover the occurrence of thee dieae and ag in animal, foodtuff and in ome cae alo in feedingtuff. In addition the report include data on antimicrobial reitance in ome zoonotic ag and indicator bacteria a well a information on epidemiological invetigation of foodborne outbreak. Complemary data on uceptible animal population in the country i alo given. The information given cover both zoonoe that are important for the public health in the whole European Union a well a zoonoe, which are relevant on the bai of the national epidemiological ituation. The report decribe the monitoring ytem in place and the previon and control trategie applied in the country. For ome zoonoe thi monitoring i baed on legal requirem laid down by the European Union legilation, while for the other zoonoe national approache are applied. The report pre the reult of the examination carried out in the reporting year. A national evaluation of the epidemiological ituation, with pecial reference to trend and ource of zoonotic infection, i given. Whenever poible, the relevance of finding in foodtuff and animal to zoonoe cae in human i evaluated. The information covered by thi report i ued in the annual European Union Summary Report on zoonoe and antimicrobial reitance that are publihed each year by EFSA. * Directive 3/ 99/ EC of the European Parliam and of the Council of December 3 on the monitoring of zoonoe and zoonotic ag, amending Deciion 9/ / EEC and repealing Council Directive 9/ 7/ EEC, OJ L 35, 7..3, p. 3 Czech Republic - 6

3 Lit of Cont TEXTFORMS 3 ANIMAL POPULATIONS 3 3. Population 3.. Information on uceptible animal population 3 DISEASE STATUS. TUBERCULOSIS, MYCOBACTERIAL DISEASES.. Mycobacterium in animal... Mycobacterium tuberculoi complex (MTC) in animal - Cattle (bovine animal) - animal ample. BRUCELLOSIS 6.. Brucella in animal... B. abortu in animal - Cattle (bovine animal) - animal ample... B. meliteni in animal - Sheep and goat - animal ample 7 3 INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS 3. SALMONELLOSIS 3.. Salmonella in foodtuff 3... Salmonella in food - Meat from bovine animal - food ample - meat 3... Salmonella in food - Meat from broiler (Gallu gallu) - food ample - meat Salmonella in food - Meat from pig - food ample - meat 3... Salmonella in food - Meat from turkey - food ample - meat Salmonella in animal Salmonella in animal - Gallu gallu (fowl) - broiler - before laughter - environmal ample Salmonella in animal - Gallu gallu (fowl) - laying hen - adult - environmal ample Salmonella in animal - Gallu gallu (fowl) - breeding flock, unpecified - adult - environmal ample 3... Salmonella in Turkey - breeding flock, unpecified - adult - breeding flock and meat production flock 5 3. CPYLOBACTERIOSIS General evaluation of the national ituation Thermophilic Campylobacter pp., unpecified - general evaluation 3.. Campylobacter in animal Thermophilic Campylobacter pp., unpecified in animal - Gallu gallu (fowl) - broiler - animal ample LISTERIOSIS Literia in foodtuff L. monocytogene in food - Other food - food ample 9 3. TRICHINELLOSIS Trichinella in animal Trichinella in animal - Pig - fattening pig - unpecified - animal ample ECHINOCOCCOSIS Echinococcu in animal E. multiloculari in animal - Foxe - wild - animal ample RABIES General evaluation of the national ituation Lyaviru (rabie) - general evaluation Lyaviru (rabie) in animal Lyaviru (rabie) in animal - All animal - animal ample Q-FEVER General evaluation of the national ituation Coxiella (Q-fever) - general evaluation VTEC Echerichia coli in foodtuff Verotoxigenic E. coli (VTEC) in food - Meat from bovine animal - carcae - food ample - carcae wab Verotoxigenic E. coli (VTEC) in food - Meat from pig - carcae - food ample - carcae wab 35 ANTIROBIAL RESISTANCE INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS 36. SALMONELLOSIS 36.. Salmonella in foodtuff Antimicrobial reitance in Salmonella Meat from bovine animal Antimicrobial reitance in Salmonella Meat from broiler (Gallu gallu) Antimicrobial reitance in Salmonella Meat from broiler (Gallu gallu) Laboratory methodology ued for idification of the microbial Antimicrobial reitance in Salmonella Meat from broiler (Gallu gallu) Laboratory ued for detection for reitance - Antimicrobial included in monitoring Antimicrobial reitance in Salmonella Meat from broiler (Gallu gallu) Laboratory ued for detection for reitance - Cut-off value ued in teting Antimicrobial reitance in Salmonella Meat from broiler (Gallu gallu) Sampling trategy ued in monitoring - Method ued for collecting data Antimicrobial reitance in Salmonella Meat from pig Antimicrobial reitance in Salmonella Meat from turkey 39.. Salmonella in animal... Antimicrobial reitance in Salmonella Gallu gallu (fowl) Laboratory methodology ued for idification of the microbial... Antimicrobial reitance in Salmonella Gallu gallu (fowl) Laboratory ued for detection for reitance - Antimicrobial included in monitoring...3 Antimicrobial reitance in Salmonella Gallu gallu (fowl) Laboratory ued for detection for reitance - Cut-off value ued in teting... Antimicrobial reitance in Salmonella Gallu gallu (fowl) Sampling trategy ued in monitoring - Method ued for collecting data...5 Antimicrobial reitance in Salmonella Turkey. CPYLOBACTERIOSIS.. Campylobacter in animal... Antimicrobial reitance in Thermophilic Campylobacter pp., unpecified Gallu gallu (fowl).3 ESCHERICHIA COLI, NON-PATHOGENIC 3.3. Echerichia coli, non-pathogenic in foodtuff Antimicrobial reitance in E.coli, non-pathogenic, unpecified Meat from broiler (Gallu gallu) Laboratory methodology ued for idification of the microbial Antimicrobial reitance in E.coli, non-pathogenic, unpecified Meat from broiler (Gallu gallu) Laboratory ued for detection for reitance - Antimicrobial included in monitoring.3..3 Antimicrobial reitance in E.coli, non-pathogenic, unpecified Meat from broiler (Gallu gallu) Laboratory ued for detection for reitance - Cut-off value ued in teting.3.. Antimicrobial reitance in E.coli, non-pathogenic, unpecified Meat from broiler (Gallu gallu) Sampling trategy ued in monitoring - Method ued for collecting data.3..5 Antimicrobial reitance in Echerichia coli, non-pathogenic Meat from broiler (Gallu gallu).3. Echerichia coli, non-pathogenic in animal Antimicrobial reitance in E.coli, non-pathogenic, unpecified Gallu gallu (fowl) Laboratory methodology ued for idification of the microbial Antimicrobial reitance in E.coli, non-pathogenic, unpecified Gallu gallu (fowl) Laboratory ued for detection for reitance - Antimicrobial included in monitoring Antimicrobial reitance in E.coli, non-pathogenic, unpecified Gallu gallu (fowl) Laboratory ued for detection for reitance - Cut-off value ued in teting Antimicrobial reitance in E.coli, non-pathogenic, unpecified Gallu gallu (fowl) Sampling trategy ued in monitoring - Method ued for collecting data Antimicrobial reitance in Echerichia coli, non-pathogenic Gallu gallu (fowl) 6 5 INFORMATION ON SPECIFIC ROBIOLOGICAL AGENTS 8 5. CRONOBACTER Cronobacter in foodtuff Cronobacter in food - Dairy product (excluding cheee) - food ample 8 5. HISTINE Hitamine in foodtuff Hitamine in food - Fih - Fihery product from fih pecie aociated with a high amount of hitidine - not enzyme maturated - food ample STAPHYLOCOCCAL ENTEROTOXINS Staphylococcal erotoxin in foodtuff Staphylococcal erotoxin in food - Cheee made from cow' milk - food ample 6 FOODBORNE OUTBREAKS 6. Outbreak Foodborne outbreak 5 ANIMAL POPULATION TABLES 53 DISEASE STATUS TABLES FOR BRUCELLA 5 Bovine brucelloi in countrie and region that do not receive Community co-financing for eradication programme 5 Ovine or Caprine brucelloi in countrie and region that do not receive Community co-financing for eradication programme 55 DISEASE STATUS TABLES FOR MYCOBACTERIUM 56 Bovine tuberculoi in countrie and region that do not receive Community co-financing for eradication programme 56 PREVALENCE TABLES 57 CPYLOBACTER 57 animal

4 food 58 COXIELLA 59 animal 59 CRONOBACTER 6 food 6 ECHINOCOCCUS 6 animal 6 ESCHERICHIA COLI 6 food 6 FLAVIVIRUS 63 animal 63 HISTINE 6 food 6 LISTERIA 65 food 65 LYSSAVIRUS 7 animal 7 SALMONELLA 75 animal 75 food 77 STAPHYLOCOCCAL ENTEROTOXINS 86 food 86 TRICHINELLA 88 animal 88 FOODBORNE OUTBREAKS TABLES 89 R TABLES FOR CPYLOBACTER 93 Campylobacter coli Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - Official ampling - R MON Campylobacter jejuni 9 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - Official ampling - R MON 9 9 R TABLES FOR SALMONELLA 95 Salmonella,,[5],:i:- - DT Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Meat, mixed meat - minced meat - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from turkey - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from pig - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Salmonella,,[5],:i:- - DT 95 Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Salmonella Ago 3 Gallu gallu (fowl) - unpecified - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Salmonella Agona Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Indutry ampling - R MON 5 5 Salmonella Anatum 6 Meat from duck - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 6 6 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 7 7 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON 8 8 Salmonella Bardo 9 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 9 9 Salmonella Bareilly Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella Bredeney Meat from turkey - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 3 3 Salmonella Coeln Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON 5 5 Salmonella Derby 6 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 6 6 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 7 7 Meat, mixed meat - meat product - Proceing plant - Surveillance - Official ampling - OTHER R MON 8 8 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 9 9 Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON Meat from pig - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from pig - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON pnl 3 3 Meat from pig - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella erica ubp. erica rough Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 6 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - R MON

5 Meat from pig - offal - Proceing plant - Surveillance - Official ampling - OTHER R MON 8 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 9 9 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 3 3 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON 3 3 Meat from pig - carcae - Proceing plant - Surveillance - Official ampling - OTHER R MON 3 3 Meat from pig - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Proceing plant - Surveillance - Official ampling - OTHER R MON 3 3 Salmonella erica, ubpecie arizonae 35 Sheep - animal under year (lamb) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Salmonella Enteritidi PT 3 36 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Indutry ampling - R MON Salmonella Enteritidi PT b Meat from turkey - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella Enteritidi PT Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Salmonella Enteritidi PT a Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - minced meat - Proceing plant - Surveillance - Official ampling - OTHER R MON 39 Salmonella Enteritidi PT Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - R MON Salmonella Enteritidi PT 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official ampling - R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 5 5 Salmonella Enteritidi PT b Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 7 Egg - table egg - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 9 9 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON 5 Gallu gallu (fowl) - broiler - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Proceing plant - Surveillance - Official ampling - OTHER R MON 5 5 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella Enteritidi PT 7 5 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 5 5 Salmonella Enteritidi PT 8 55 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat, mixed meat - meat product - Proceing plant - Surveillance - Official ampling - OTHER R MON Sheep - animal over year - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON 59 Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 6 6 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - R MON 6 6 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Gallu gallu (fowl) - par breeding flock for broiler production line - Farm - Control and eradication programme - Official ampling - R MON 6 6 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON 65 Gallu gallu (fowl) - laying hen - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Indutry ampling - R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official ampling - R MON Gallu gallu (fowl) - unpecified - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Gallu gallu (fowl) - unpecified - Farm - Clinical invetigation - Indutry ampling - OTHER R MON 7 7 Gallu gallu (fowl) - broiler - Farm - Clinical invetigation - Indutry ampling - OTHER R MON 7 7 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 7 Salmonella Enteritidi RDNC 7 73 Gallu gallu (fowl) - broiler - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Salmonella Enteritidi U 7 Duck - meat production flock - Farm - Clinical invetigation - Indutry ampling - OTHER R MON 7 7 Salmonella Gallinarum biovar Pullorum 75 Gallu gallu (fowl) - laying hen - Farm - Clinical invetigation - Indutry ampling - OTHER R MON

6 75 Salmonella Give Duck - meat production flock - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON Salmonella Glouceter 78 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella I 6,7:-:,5 Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Salmonella Indiana Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 8 8 Salmonella Infanti 8 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 8 8 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 8 8 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - R MON pnl Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER R MON 88 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON pnl Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 9 9 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - R MON 9 9 Meat from broiler (Gallu gallu) - minced meat - Proceing plant - Surveillance - Official ampling - OTHER R MON 9 9 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 9 9 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official ampling - R MON 96 Meat from pig - minced meat - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella Kucky Meat from turkey - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Meat from turkey - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 3 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - R MON Gallu gallu (fowl) - par breeding flock for broiler production line - Farm - Control and eradication programme - Official ampling - R MON Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON 6 6 Salmonella Mbandaka 7 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 7 7 Salmonella Montevideo 8 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 8 8 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 9 9 Salmonella Muenter Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Salmonella Newport Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from turkey - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 3 3 Meat from broiler (Gallu gallu) - meat product - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 5 5 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON 6 6 Meat from turkey - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON 7 7 Salmonella Ohio 8 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 8 8 Meat from broiler (Gallu gallu) - minced meat - Proceing plant - Surveillance - Official ampling - OTHER R MON 9 9 Meat from broiler (Gallu gallu) - minced meat - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella Putten 3 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON

7 Salmonella Rien Meat, mixed meat - minced meat - Proceing plant - Surveillance - Official ampling - OTHER R MON Meat from pig - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON 5 5 Salmonella Senftenberg 6 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 6 6 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON 7 7 Salmonella Stanleyville 8 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 8 8 Salmonella Typhimurium DT 9 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON 9 9 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 3 3 Salmonella Typhimurium DT 3 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 3 3 Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER R MON 3 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - R MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON 3 3 Salmonella Typhimurium DT Meat from broiler (Gallu gallu) - meat product - Proceing plant - Surveillance - Official ampling - OTHER R MON 35 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella Typhimurium DT 37 Meat, mixed meat - meat product - Proceing plant - Surveillance - Official ampling - OTHER R MON Sheep - animal under year (lamb) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Salmonella Typhimurium DT 5a 39 Meat from turkey - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - R MON Salmonella Typhimurium DT 93 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER R MON Salmonella Typhimurium DT 39 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - R MON Salmonella Typhimurium DT 8 3 Meat from pig - offal - Proceing plant - Surveillance - Official ampling - OTHER R MON Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER R MON Salmonella Typhimurium DT 85 Geee - meat production flock - Farm - Clinical invetigation - Indutry ampling - OTHER R MON 5 Salmonella Virchow 6 Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER R MON 6 6 R TABLES FOR ESCHERICHIA COLI 7 Echerichia coli, non-pathogenic, unpecified 7 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 7 7 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - Official ampling - ESBL MON 8 8 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 9 9 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - Official ampling - ESBL MON 5 5 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 5 5 Meat from broiler (Gallu gallu) - freh - Retail - Monitoring - Official ampling - ESBL MON 5 5 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - Official ampling - R MON pnl Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - Official ampling - R MON 5 5 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - Official ampling - ESBL MON pnl Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - Official ampling - ESBL MON OTHER R TABLES 57 ESBL 58 LATEST TRANSMISSIONS

8 ANIMAL POPULATIONS The relevance of the finding on zoonoe and zoonotic ag ha to be related to the ize and nature of the animal population in the country. Population.. Information on uceptible animal population Source of information Enter SubTitle order number and then Subtitle Text Date the figure relate to and the cont of the figure Number of animal and holding related to National evaluation of the number of uceptible population and trend in thee figure Number of cattle holding were 9 53 in total related to and number of animal were 3 57 in total. It mean that cattle population trend i imilar in comparion with year 5. In hore, goat and heep population i evid increing trend in number of kept animal. Number of pig holding i lower than in 5 and the ame ituation i with regard number of animal. Czech Republic - 6 3

9 DISEASE STATUS. TUBERCULOSIS, MYCOBACTERIAL DISEASES.. Mycobacterium in animal... Mycobacterium tuberculoi complex (MTC) in animal - Cattle (bovine animal) - animal ample Statu a officially free of bovine tuberculoi during the reporting year The ire country free The Czech Republic i free of Bovine tuberculoi caued by M. bovi ince 967 on the national level and from i declared a officially free in accordance with EU legilation on the bae of Commiion Deciion /3/EC. Free region The whole territory of the Czech Republic i declared a a officially free of tuberculoi (M. bovi) in relation to bovine herd. Additional information During the reporting year 6 there wa no occurrence and/or outbreak of bovine tuberculoi caued by Mycobacterium bovi in bovine animal. Monitoring ytem Sampling trategy The ampling trategy and monitoring ytem i in accordance with Directive 6/3/EEC a amended. Frequency of the ampling Tuberculoi Alergenodiagnoi imple kin tet (antigen "Bovitubal" M. bovi 8 IU). Data of the lat kin tet mut be checked prior to kin tet in order to oberve pecified time period between individual examination. a) Examination of % of the female bovine animal aged over month in each region (examination of all animal, maximum animal per holding); b) Animal imported from third countrie (excluding laughter animal) examination of female animal and breeding bull over 6 week of age. The examination mut be carried out a oon a poible after arrival of animal to the place of detination with repect to evual previou tuberculin tet; c) Animal moved from Member State not having tatu of bovine tuberculoi officially free country or region (excluding laughter animal) - examination of female animal and breeding bull over 6 week of age. The examination mut be carried out a oon a poible after arrival of animal to the place of detination with repect to evual previou tuberculin tet; Breeding bull in BBRH examination within 8 day prior to baic election; e) Breeding bull prior to admiion to emen collection cre examination in accordance with Annex to Decree No. 38/3 Coll; f) Breeding bull in emen collection cre x per year examination in accordance with Annex to Decree No. 38/3. Coll. Type of pecimen taken Skin tet Method of ampling (decription of ampling technique) Czech Republic - 6

10 The place of antigen application i ituated at the border of the anterior and middle third of the neck. The kin mut be without pathological change, equally thick with the poibility of an eay cutaneou drape formation. The place of tuberculin adminitration i perfectly cut and cleaned. The cutaneou drape i formed with the thumb and the point finger and it thickne i after cutimetre meauring recorded. The doage of. ml of tuberculin i applicated by mean of a hort terile needle, bevel edge outward, with graduated yringe charged with tuberculin, inerted obliquely into the deepet layer of the kin. The right reaction after intradermal adminitration - the papula formation in the place of allergen inoculation - mut be detected by palpation. If the tuberculin wa not adminitered intradermally, it i poible to repeat the adminitration in the ame place in the precribed doage. If the kin i injured during cutting or if kin change are determined before tuberculin adminitration, it i neceary to inoculate tuberculin on another place of the ame neck ide. The origin place i cancelled with the hair cut. Cae definition Negative reaction: If there i appar only bordered welling with the cutaneou drape trengthening of max. mm without clinical ymptom a diffuion or large welling, exudation, necroi, painfulne or inflammation reaction of the correponding lymphatic veel or lymphatic node. Dubiou reaction: If there i appar no clinical ymptom tated in item a) but the cutaneou drape trengthening i higher than mm but lower than mm. Poitive reaction: If there are appar clinical ymptom tated in item a) or the cutaneou drape in the place of application i thicker by mm or more. Diagnotic/analytical method ued Simple kin tet ha been performed with tuberculin BOVITUBAL 8 IU/ml (Bioveta, CZ) which contain tuberculin protein from Mycobacterium bovi (train AN 5). The doe for one animal i,ml. The diagnotic method i in accordance with recommendation OIE. Vaccination policy Vaccination i trictly prohibited. Other previve meaure than vaccination in place All laughtered bovine animal are under veterinary control. The official pot mortem veterinary examination i carried out in laughterhoue by the official veterinarian in accordance with EU legilation. Control program/mechanim The control program/trategie in place The control of bovine tuberculoi i performed in accordance with Directive 6/3/EC a amended. Meaure in cae of the poitive finding or ingle cae In the cae of poitive reult of examination the appropriate Regional Veterinary Adminitration iue extraordinary veterinary meaure in accordance with Veterinary Act (CZ legilation) and EU legilation. Notification ytem in place Notification ytem i laid down by the Act No. 66/999 Coll, on veterinary care and amending certain related law (Veterinary Act), a amended. Reult of the invetigation If the reult of invetigation i poitive, the peron reponible for the laboratory carrying out the examination, the peron carrying out the examination or the owner of the animal hall notify the reult to the compet authority. National evaluation of the rec ituation, the trend and ource of infection Czech Republic - 6 5

11 In the Czech Republic bovine tuberculoi wa uppreed in frame of the nationwide anitation program ( ) on October 968. The pot-eradication period ( ) wa characterized by the extinction of reervoir ource. Currly only the poradic cae of the bovine tuberculoi incidence have been recorded. In 98, 987 to 99, 993 and 996 any bovine tuberculoi incidence wa not found. Thereat in other year, from 98 to 995, at the mot three outbreak of tuberculoi ever appeared in cattle. The participation of the infected animal in individual tock wa very low and never exceeded 5 to % of animal. In 97 to 995 the Mycobacterium bovi infection wa alo diagnoed in other 9 animal (zoo, wild live, backyar and in ten milk pecimen. By coure of the O.I.E. (International Animal Health Code,chapter 3..3.) definition the territory of the Czech Republic i free from bovine tuberculoi (the prevalence up to,% of infected cattle tock). Relevance of the finding in animal to finding in foodtuff and to human cae (a a ource of infection) There i an paradox ituation becaue human become rik for animal, mainly worker from eater third countrie might be ource of infection for animal. Additional information In the framework of the health control paid by the tate, bovine tuberculoi i currly monitored in the CR a follow: ingle tuberculin tet, imultaneou tuberculin tet, laboratory examination (ection, hitological invetigation and bacteriological invetigation), erological invetigation.. BRUCELLOSIS.. Brucella in animal... B. abortu in animal - Cattle (bovine animal) - animal ample Statu a officially free of bovine brucelloi during the reporting year The ire country free The Czech Republic i free of bovine brucelloi ince 96 at the natinal level and ince i declared a officially free of bovine bruceloi according to Commiion Deciion /3/EC. Additional information During the reporting year 6 there wa no occurrence and/or outbreak of bovine brucelloi in the whole territory of the Czech Republic. Monitoring ytem Sampling trategy Sample are taken from:. All breeding bull, all abortion animal - blood ample.. % of cattle - all female animal from age month, all female - maximum head per holding - blood ample. 3. Abortion foetue in indicated caae.. Imported animal from third countrie and animal coming from EU tate/region not officially free from bruceloi (except for laughter animal) - teting of female animal older than month of age and breeding bull. Frequency of the ampling Czech Republic - 6 6

12 Sampling cheme: ) Brucelloi erological examination(rbt or ELISA): a) breeding bull in breeding bull rearing houe - examination within 8 day prior to baic election; b) breeding bull prior to admiion to emen collection cre - examination in accordance with Annex to Decree No. 38/3 Coll.; c) breeding bull in emen collection cre x per year examination in accordance with Annex to Decree No. 38/3 Coll.; all aborting cow are erologicaly teted for Brucelloi after abortion. ) Brucelloi erological examination (ELISA): a) animal imported from third countrie (excluding laughter animal) examination of female animal over month of age and breeding bull. The examination mut be carried out at mot month after arrival of animal to the place of detination; b)animal moved from Member State/region not officially free from bovine brucelloi (excluding laughter animal) - examination of female animal over month of age and breeding bull. The examination mut be carried out at mot month after arrival of animal to the place of detination; c) Blood ample from % of all female animal older moth x per year are teted for Brucelloi (all female animal, maximum animal per holding in each region). 3) Abortion and amnia examination in indicated cae. Type of pecimen taken Blood, abortion foetu. Diagnotic/analytical method ued The diagnotic method are ued in accordance with Directive 6/3/EEC, Regulation /6/EEC. RBT, Complem fixation tet, ELISA, low agglutination. Vaccination policy Vaccination i trictly prohibited. Other previve meaure than vaccination in place Control of animal movem between region and control of imported animal.... B. meliteni in animal - Sheep and goat - animal ample Statu a officially free of ovine brucelloi during the reporting year Statu a officially free of caprine brucelloi during the reporting year The ire country free The whole teritory of the Czech Republic i officially free of Sheep and goat bruceloi in accordance with Commiion Deciion No. 3//EC. The ire country free Enter SubTitle order number and then Subtitle Text Free region All region in the Czech republic are free of ovine bruceloi (B. meliteni) and the dieae ha never been found in the Czech Republic. Monitoring ytem Sampling trategy The ampling trategy wa done by State Veterinary Adminitration in Methodology of control of animal health which i laid down in accordance with Veterinary Act No. 66/999 Coll. a amended. Czech Republic - 6 7

13 Sampling trategy The ampling trategy wa done by State Veterinary Adminitration in Methodology of control of animal healt which i laid down in accordance with Veterinary Act No. 66/999 Coll. a amended. Frequency of the ampling Ovine and caprine brucelloi (B. meliteni): a) Licened breeding ram examination x per year in accordance with Annex 9 to Decree No. 38/3 Coll; b) Holding (herd) producing young breeding animal where the teting i carried out annually. Repreative number of animal hall include:. all non-catrated male animal over 6 month of age;. 5% of female animal over month of age of at leat 5 female animal (all animal teted in holding containing le than 5 animal); c) Aborting ewe are erologicaly teted once after abortion; Abortion or amnia examination in indicated cae. Frequency of the ampling Caprine brucelloi (B. meliteni)-: a) Aborting goat erological examination after abortion; b) Licened breeding buck examination x per year in accordance with Annex 9 to Decree No. 38/3 Coll; c) holding (herd) producing young breeding animal where the teting i carried out annually. Repreative number of animal hall include:. all non-catrated male animal over 6 month of age;. 5% of female animal over month of age of at leat 5 female animal (all animal teted in holding containing le than 5 animal); Type of pecimen taken Blood and foetue. Type of pecimen taken Blood Method of ampling (decription of ampling technique) The method of ampling and teting i in according with Annex of the Council Deciion 9//EEC Method of ampling (decription of ampling technique) The method of ampling i in according with Annex of the Council Deciion 9//EEC. Diagnotic/analytical method ued The diagnotic method were ued in accordance with Directive 6/3/EEC and Regulation /6/EEC. RBT, CFT, ELISA and low agglutination. Vaccination policy Vaccination i trictly prohibited. Vaccination policy Vacination i trictly prohibited. Other previve meaure than vaccination in place Monitoring of animal health ituation and control of imported animal. National evaluation of the rec ituation, the trend and ource of infection The dieae ha never been recorded in the Czech Republic. Czech Republic - 6 8

14 Czech Republic - 6 9

15 3 INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS Zoonoe are dieae or infection, which are naturally tranmiible directly or indirectly between animal and human. Foodtuff erve often a vehicle of zoonotic infection. Zoonotic ag cover virue, bacteria, fungi, paraite or other biological itie that are likely to caue zoonoe. 3. SALMONELLOSIS 3.. Salmonella in foodtuff 3... Salmonella in food - Meat from bovine animal - food ample - meat Monitoring ytem Sampling trategy At laughterhoue and cutting plant SVA: The laughterhoue are preelected o that the ire area of the Czech Republic wa covered. Sampling at laughterhoue for cattle i performed on a random bai from urface of five carcae; the ample are taken from four pecified ite of carcae before chilling. If the amount of five cattle head i not avalaible on the day of ampling, then a maller lot will be elected for it. Preferably it hould be enured that each epidemiological unit (i.e. farm) i ampled at given laughterhoue only once per year. A non-detructive method with an abraive ponge i ued, according to ISO 76. At meat proceing plant SVA: The ample are taken in the ordinary urveillance, depending on the procedure baed on the hazard analyi. The final product are ampled at the end of production. There i no official national program for the monitoring of Salmonella pp. at proceing plant and retail. Control in retail and proceing plant are performed according to Commiion Regulation (EC) No 73/5 of 5 November 5 on microbiological criteria for foodtuff. Sample are taken by compet authority from all region of the Czech Republic within the year a part of an official ampling, i.e. it i performed by inpector from the Regional Veterinary Adminitration, the ample are then analyed at the State Veterinary Intitute. Frequency of the ampling At laughterhoue and cutting plant SVA: Once a month at preelected laughterhoue. At meat proceing plant SVA: Sampling ditributed evenly throughout the year. Type of pecimen taken At laughterhoue and cutting plant Czech Republic - 6

16 SVA: Carcae wab. At meat proceing plant SVA: Final product. Method of ampling (decription of ampling technique) At laughterhoue and cutting plant SVA: Sampling at laughterhoue for cattle i performed on a random bai from urface of five carcae; the ample are taken from four pecified ite of carcae (rump, flank, briket, neck) before chilling. Each wab i performed from area- cm. A nondetructive method with an abraive ponge i ued. The ample are aeptically removed and placed aeptically into a ample container and tranferred to the State Veterinary Intitute. At meat proceing plant SVA: The ample - meat product (final product), are placed aeptically into a ample container and tranfer to the State Veterinary Intitute. Definition of poitive finding At laughterhoue and cutting plant SVA: Preence of Salmonella pp. in cm. At meat proceing plant SVA: Preence of Salmonella in 5/ g (depending on food category a defined in Commiion Regulation (EC) No 73/5) of ample. Diagnotic/analytical method ued At laughterhoue and cutting plant SVA: EN/ISO 6579 At meat proceing plant SVA: EN/ISO 6579 Previve meaure in place SVA: Control of the procedure baed on the HACCP principle and GHP ytem. Control program/mechanim The control program/trategie in place Czech Republic - 6

17 SVA: The compet authority take meaure according to the legilation in force and defined cae are reported into the Rapid Alert Sytem for Food and Feed. Rec action taken to control the zoonoe SVA, NIPH and CAFIA carry out monitoring and control program in the whole food chain and take appropriate meaure according to the legilation in force to enure afe foodtuff. Meaure in cae of the poitive finding or ingle cae SVA: In the cae of poitive reult of the invetigation the compet authority take meaure to prev preading of the infection to the food chain. Notification ytem in place SVA: The poitive reult of the bacteriological tet ha to be reported to the appropriate Regional Veterinary Adminitration (RVA) and the RVA ha oblige to take appropriate meaure. The poitive reult are reported to the RVA from State Veterinary Intitute which make the laboratory analyi. National evaluation of the rec ituation, the trend and ource of infection SVA: The prevalence of the Salmonella pp. by bovine carcae lightly decreaed in comparion with the lat year Salmonella in food - Meat from broiler (Gallu gallu) - food ample - meat Monitoring ytem Sampling trategy At laughterhoue and cutting plant SVA: The laughterhoue are preelected o that the ire area of the Czech Republic wa covered. Sampling at laughterhoue for broiler i performed on a random bai, it i taken of five pooled ample of kin from the neck, after chilling, from the lot of broiler about 5 piece and more. If one the day of ampling a maller than abovemioned number of piece it laughtered, then a maller lot will be ampled. Every epidemiological unit (i.e. flock) may be ampled only once per year. At meat proceing plant SVA: The ample are taken in the ordinary urveillance, depending on the procedure baed on the hazard analyi. The final product are ampled at the end of production. There i no official national program for the monitoring of Salmonella pp. at proceing plant and retail. Control in retail and proceing plant are performed according to Commiion Regulation (EC) No 73/5 of 5 November 5 on microbiological criteria for foodtuff. Sample are taken by compet authoritie from all region of the Czech Republic within the year a part of an official ampling, i.e. it i performed by inpector from the Regional Veterinary Adminitration, the ample are then analyed at the State Veterinary Intitute. Frequency of the ampling At laughterhoue and cutting plant SVA: Once a month at preelected laughterhoue. At meat proceing plant Czech Republic - 6

18 SVA: Sampling ditributed evenly throughout the year. Type of pecimen taken At laughterhoue and cutting plant SVA: Neck kin. At meat proceing plant SVA: Final product. Method of ampling (decription of ampling technique) At laughterhoue and cutting plant SVA: Sampling at laughterhoue for poultry i carried out from carcae after chilling; 5 neck kin ample are taken on random from 5 broiler carcae. A piece of approximately g from neck kin hall be obtained from each carca.the neck kin ample from three carcae are pooled before examination to form 5 x 5 g final ample. At meat proceing plant SVA: The ample - meat product (final product), are placed aeptically into a ample container and tranfer to the State Veterinary Intitute. Definition of poitive finding At laughterhoue and cutting plant SVA: Preence of Salmonella in 5/ g (depending on food category a defined in Commiion Regulation (EC) No 73/5) of ample. At meat proceing plant SVA: Preence of Salmonella in 5/ g (depending on food category a defined in Commiion Regulation (EC) No 73/5) of ample. Diagnotic/analytical method ued At laughterhoue and cutting plant SVA: EN/ISO 6579 At meat proceing plant SVA: EN/ISO 6579 Previve meaure in place SVA: Control of the procedure baed on the HACCP principle and GHP ytem. Czech Republic - 6 3

19 Control program/mechanim The control program/trategie in place SVA: The compet authority take meaure according to the legilation in force and defined cae are reported into the Rapid Alert Sytem for Food and Feed. Rec action taken to control the zoonoe SVA, NIPH and CAFIA carry out monitoring and control program in the whole food chain and take appropriate meaure according to the legilation in force to enure afe foodtuff. Meaure in cae of the poitive finding or ingle cae SVA: In the cae of poitive reult of the invetigation the compet authority take meaure to prev preading of the infection to the food chain. Notification ytem in place SVA: The poitive reult of the bacteriological tet ha to be reported to the appropriate Regional Veterinary Adminitration (RVA) and the RVA ha oblige to take appropriate meaure. The poitive reult are reported to the RVA from the State Veterinary Intitute which make the laboratory analyi. National evaluation of the rec ituation, the trend and ource of infection SVA: The prevalence of the Salmonella pp. in broiler carcae coniderably decreaed in comparion with the lat year Salmonella in food - Meat from pig - food ample - meat Monitoring ytem Sampling trategy At laughterhoue and cutting plant SVA: At preelected laughterhoue the veterinary inpector take: Once a month ample from -5 carcae, from one for teting. The ditribution of the number of ample mut enure that in one calendar year up to 9 ample are taken (e.g. once a month ample from the carca and in one calendar month 5 ample are taken (total 9 per year). A maximum of 5 ample per month i acceptable. If on the day of ampling a maller than abovemioned number of piece it laughtered, then a maller lot will be ampled. Annex I, Section IV, Chapter IX, letter G. point a) of the Regulation (EC) No 85/ allow for maller laughterhoue to reduce the number of ample baed on rik aem. It i poible to reduce the number of ample at laughterhoue, which according to Decree No 8/9 are conidered a mall or ubject to approval and regitration with limitation (e.g. a daily laughter capacity). The lit of laughterhoue, in which the number of ample i reduced, i to the Regional Veterinary Adminitration regularly on January 5 of the calendar year. The above mioned ample do replace the ample officially taken to verify the proce hygiene criteria according to Commiion Regulation (EC) No 73/5 (code.. Salmonella in pig carcae). At meat proceing plant SVA: The ample are taken in the ordinary urveillance, depending on the procedure baed on the hazard analyi. The final product are ampled at the end of production. There i no official national program for the monitoring of Salmonella pp. in retail and proceing plant. Control are performed according to Commiion Regulation (EC) No 73/5 of 5 November 5 on microbiological criteria for foodtuff. Sample are taken by compet authority from all region of the Czech Republic within the year a part of an official ampling, i.e. it i performed by inpector from the Regional Veterinary Adminitration, the ample are then analyed at the State Veterinary Intitute. Czech Republic - 6