The Report referred to in Article 5 of Directive 92/117/EEC

Transcription

1 LITHUANIA The Report referred to in Article 5 Directive 92/117/EEC TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN HUMANS, FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS including information on foodborne outbreaks and antimicrobial resistance in zoonotic agents IN 2004

2 INFORMATION ON THE REPORTING AND MONITORING SYSTEM Country: Lithuania Reporting Year: 2004 Institutions and laboratories involved in monitoring: Laboratory name Description Contribution Lithuania 2004

3 PREFACE This report is submitted to the European Commission in accordance with Article 5 Council Directive 92/117/EEC 1. The information has also been forwarded to the European Food Safety Authority (EFSA). The report contains information on trends and sources zoonoses and zoonotic agents in Lithuania during the year The information covers the occurrence these diseases and agents in humans, animals, foodstuffs and in some cases also in feedingstuffs. In addition the report includes data on antimicrobial resistance in some zoonotic agents and commensal bacteria as well as information on epidemiological investigations foodborne outbreaks. Complementary data on susceptible animal populations in the country is also given. The information given covers both zoonoses that are important for the public health in the whole European Community as well as zoonoses, which are relevant on the basis the national epidemiological situation. The report describes the monitoring systems in place and the prevention and control strategies applied in the country. For some zoonoses this monitoring is based on legal requirements laid down by the Community Legislation, while for the other zoonoses national approaches are applied. The report presents the results the examinations carried out in the reporting year. A national evaluation the epidemiological situation, with special reference to trends and sources zoonotic infections, is given. Whenever possible, the relevance findings in foodstuffs and animals to zoonoses cases in humans is evaluated. The information covered by this report is used in the annual Community Summary Report on zoonoses that is published each year by EFSA. 1 Council Directive 92/117/ECC 17 December 1992 concerning measures for protection against specified zoonoses and specified zoonotic agents in animals and products animal origin in order to prevent outbreaks foodborne infections and intoxications, OJ L 62, , p. 38 Lithuania 2004

4 LIST OF CONTENTS 1. ANIMAL POPULATIONS 1 2. INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS SALMONELLOSIS General evaluation the national situation Salmonellosis in humans Salmonella in foodstuffs Salmonella in animals Salmonella in feedstuffs Salmonella serovars and phagetype distribution Antimicrobial resistance in Salmonella isolates CAMPYLOBACTERIOSIS General evaluation the national situation Campylobacteriosis in humans Campylobacter in foodstuffs Campylobacter in animals Antimicrobial resistance in Campylobacter isolates LISTERIOSIS General evaluation the national situation Listeriosis in humans Listeria in foodstuffs VEROCYTOTOXIC ESCHERICHIA COLI General evaluation the national situation Verocytotoxic Escherichia coli in humans Pathogenic Escherichia coli in foodstuffs Pathogenic Escherichia coli in animals TUBERCULOSIS General evaluation the national situation Tuberculosis in humans Mycobacterium in animals BRUCELLOSIS General evaluation the national situation Brucellosis in humans Brucella in foodstuffs Brucella in animals YERSINIOSIS General evaluation the national situation Yersiniosis in humans Yersinia in foodstuffs Yersinia in animals TRICHINELLOSIS General evaluation the national situation Trichinellosis in humans Trichinella in animals ECHINOCOCCOSIS 95 Lithuania 2004

5 General evaluation the national situation Echinococcosis in humans Echinococcus in animals TOXOPLASMOSIS General evaluation the national situation Toxoplasmosis in humans Toxoplasma in animals RABIES General evaluation the national situation Rabies in humans Lyssavirus (rabies) in animals INFORMATION ON SPECIFIC INDICATORS OF ANTIMICROBIAL 111 RESISTANCE 3.1. E. COLI INDICATORS General evaluation the national situation Antimicrobial resistance in Escherichia coli isolates FOODBORNE OUTBREAKS 115 Lithuania 2004

6 1. ANIMAL POPULATIONS The relevance the findings on zoonoses and zoonotic agents has to be related to the size and nature the animal population in the country. Table 14.1 Susceptible animal populations: number herds and holdings rearing animals * Only if different than current reporting year Animal species Category animals Number herds or flocks Number holdings Year* Year* Cattle (bovine animals) dairy cows and heifers in total Ducks in total 2 Gallus gallus mixed flocks/holdings (1) broilers (2) 1183 laying hens 83 parent birds for meat production line 49 parent birds for egg production line 5 in total Geese in total 1 Goats in total 3665 Pigs sows and gilts fattening pigs mixed herds in total Sheep in total 2630 Solipeds horses in total Turkeys in total 3 Farmed reindeers in total 0 Farmed wild boars in total 0 Farmed deer in total 0 (1): 182 flocks montly (2): 182 flocksat one period growth Lithuania

7 Table 14.2 Susceptible animal populations: number animals * Only if different than current reporting year Animal species Category animals Livestock numbers (live animals) Number slaughtered animals Year* Year* Cattle (bovine animals) calves (under 1 year) dairy cows and heifers meat production animals in total Ducks in total Gallus gallus parent birds in total broilers (1) laying hens parent birds for meat production line parent birds for egg production line in total Geese in total Goats in total 7112 Pigs sows and gilts breeding animals fattening pigs in total Sheep in total Solipeds horses in total Turkeys in total Farmed reindeers in total 0 Farmed wild boars in total 0 Farmed deer in total 0 (1): at one period growth Footnote Data on the 1st January 2004 Lithuania

8 2. INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS Zoonoses are diseases or infections, which are naturally transmissible directly or indirectly between animals and humans. Foodstuffs serve ten as vehicles zoonotic infections. Zoonotic agents cover viruses, bacteria, fungi, parasites or other biological entities that are likely to cause zoonoses. Lithuania

9 2.1. SALMONELLOSIS General evaluation the national situation Salmonellosis in humans A. Salmonellosis in humans Reporting system in place for the human cases Health minister's order regulates information providing system on communicable diseases and it's list. Clinicist informs territorial public healthcare institution about suspected or probable or confirmed case in 12 hours by phone, in 72 hours sends an urgent report. When diagnosis is changed clinicist informs territorial public healthcare institution in 12 hours. Territorial public healthcare institutions registrate every case in standart form. In the end every month data on morbidity communicable diseases are summerised and send to national level CCDPC. CCDPC publishes monthly bulletin morbidity in infectious diseases and sends it to regional public healthcare centers, State public healthcare service, State food and veterinary service, boardering countries WHO, EU communicable diseases surveillance network. Every year territorial public healthcare institutions provide annual statistical data on extended epidemiological investigations. Case definition Clinical picture compatible with salmonellosis, isolation Salmonella (nontyphi, nonparatyphi) from a clinical specimen. Diagnostic/analytical methods used Methods used: Bacteriological culture Quality assurance procedures: Internal quality control sistem: a) standart media quality control procedures b) standart antisera control procedures c) standart antimicrobial susceptibility testing control procedures External "PT" Quality Assessment Programmes. Labquality Helsinki Finland. Reference:WHO Manual for the laboratory identification and antimicrobial susceptibility testing bacterial pathogens public health concern in the developing world Notification system in place Every probable, suspected, or confirmed case is registered in personal healthcare institution according Health minister's order and is informed to territorial public healthcare institution where cases are registered. All detected cases are reported to the national level CCDPC and cases are registered in State register for communicable diseases. History the disease and/or infection in the country Lithuania

10 Since 1959 there are detected 2 peaks salmonellosis incidence: S.typhimurium outspread through milk formula produced in centralised milk formula kitchens and in children departments hospitals. Since 1989 to 1998 was noticed second peak incidence where S. enteritidis dominated. Results the investigation Territorial public healthcare institutions perform an epidemiological investigation salmonellosis clusters according to standartised protocol. These protocols are kept in territorial public healthcare institutions. Computerised communicable diseases reporting system is implemented in two regional public health centers (Vilnius, Kaunas). National evaluation the recent situation, the trends and sources infection Since 1998 is a trend decrease in incidence. Incidence dicreased from 68,8/ in 1998 to 33,3/ in In 2004 incidence rate increased to 54/ per population. Last years mostly ( 85%) there have been registered sporadical cases. During last years the main reason salmonellosis is chicken, other poultry, eggs and their products. Relevance as zoonotic disease Between all diarrhoea diseases salmonellosis consist about 10%. About 70% patients are infected from homemade food. Lithuania

11 Table A Salmonellosis in man species/serotype distribution Cases Cases Inc Autochtone cases Autochtone Inc Imported cases Imported Inc unknown status Salmonella S. Bovismorbificans 1 1 S. Braenderup 3 3 S. Derby 2 2 S. Dublin 1 1 S. Enteritidis S. Glostrup 7 7 S. Hadar 1 1 S. Infantis S. Kentucky 1 1 S. Oranienburg 1 1 S. Tshiongwe 3 3 S. Typhimurium S. Virchow 1 1 Salmonella spp clinical case Footnote General incidence rate per population was 54,4. Lithuania

12 Table B Salmonellosis in man age distribution S. Enteritidis S. Typhimurium Salmonella spp. Age Distribution All M F All M F All M F <1 year to 4 years(1) to 14 years(2) to 24 years(3) to 44 years(4) to 64 years(5) years and older(6) Age unknown Total : (1) : 1 to 3 years (2) : 4 to 14 years (3) : 15 to 29 years (4) : 30 to 49 years (5) : 50 to 59 years (6) : 60 years and older Lithuania

13 Table Salmonellosis in man seasonal distribution S. Enteritidis S. Typhimurium Salmonella spp. Month Cases Cases Cases January February March April May(1) June July(2) August(3) September(4) October November December not known Total : (1) : 1 clinical case (2) : 3 clinical cases (3) : 1 clinical case (4) : 20 clinical cases Lithuania

14 Lithuania 2004 Report on trends and sources zoonoses Salmonella in foodstuffs Table Salmonella sp. in meat and meat products Source information Remarks Epidemiological unit Sample weight Units tested Units positive S. Enteritidis S. Typhimurium S. group B S. group D Bovine meat fresh Ḻabs. at processing plant at retail Labs. sample 25g 3 sample 25g 8 minced meat at processing plant Labs. at retail Labs. sample 25g 2 sample 25g 8 meat products nonreadytoeat at retail Labs. sample 25g 3 readytoeat at processing plant Labs. sample 25g Pig meat fresh at processing plant (1) Labs. at retail (2) Labs. sample 25g sample 25g minced meat at processing plant Labs. at retail Labs. sample 25g 6 sample 25g 18 meat products nonreadytoeat at processing plant Labs. at retail Labs. sample 25g 3 sample 25g 10 Lithuania

15 Lithuania 2004 Report on trends and sources zoonoses readytoeat at processing plant Labs. at retail Labs. sample 25g sample 25g 13 Broiler meat fresh at processing plant Labs. at retail Labs. sample 25g 3 sample 25g 23 minced meat at retail Labs. sample 25g 2 meat products nonreadytoeat at processing plant Labs. at retail Labs. sample 25g 9 sample 25g readytoeat at processing plant Labs. at retail Labs. sample 25g 8 sample 25g 22 Turkey meat fresh at processing plant Labs. sample 25g 1 minced meat at processing plant Labs. sample 25g 1 meat products nonreadytoeat at processing plant Labs. sample 25g 2 readytoeat at processing plant Labs. at retail Labs. sample 25g 2 sample 25g 1 Other meat fresh at processing plant Labs. sample 25g 1 minced meat Lithuania

16 Lithuania 2004 Report on trends and sources zoonoses at retail Labs. sample 25g 4 Mixed meat minced meat at processing plant Labs. at retail Labs. Other animals or mixed meat sample 25g 2 sample 25g 11 meat products nonreadytoeat at processing plant Labs. at retail Labs. sample 25g 11 sample 25g 4 readytoeat at processing plant Labs. at retail Labs. (1) : Together with subpoducts (2) : Together with subpoducts Labs. Laboratories sample 25g sample 25g 60 Lithuania

17 Lithuania 2004 Report on trends and sources zoonoses Table Salmonella sp. in other food Source information Remarks Epidemiological unit Sample weight Units tested Units positive S. Enteritidis S. Typhimurium S. group D cow milk raw Ḻaboratories sample 25ml 6 Dairy products readytoeat Laboratories sample 25g/ml 153 Table eggs at retail Laboratories Egg products Ḻaboratories sample 25g 20 sample 25g 1 Fishery products fish Other food Ḻaboratories Ḻaboratories sample 25g 32 sample 25g/ml Lithuania

18 Salmonella in animals Table Salmonella sp. in Poultry breeding flocks (Gallus gallus) Source information Remarks Epidemiological unit Flocks tested Flocks positive S. Enteritidis S. Typhimurium Gallus gallus AHD Flock 6 0 parent breeding flocks for egg production line dayold chicks AHD Flock 4 0 AHD Flock 6 0 during production period during rearing period AHD Flock 4 0 parent breeding flocks for AHD Flock 72 0 meat production line dayold chicks AHD Flock 35 0 during rearing period AHD Flock 48 0 AHD during production period Flock 52 0 parent breeding flocks, unspecified AHD Flock 1 0 Lithuania

19 Lithuania 2004 Report on trends and sources zoonoses Table Salmonella sp. in other commercial poultry Source information Remarks Epidemiological unit Flocks tested Flocks positive S. Enteritidis S. Typhimurium S. Infantis S. group B S. group C Gallus gallus laying hens dayold chicks Labs. during rearing period Labs. during production period Labs. unspecified Labs. Agent id. Agent id. Agent id. Agent id. Flock Flock Flock Flock broilers dayold chicks Labs. during rearing period Labs. unspecified Labs. Agent id. Agent id. Agent id. Flock Flock Flock Footnote State Food and Veterinary Service; Labs. Laboratories Agent id. Agent identification Lithuania

20 Lithuania 2004 Report on trends and sources zoonoses Table Salmonella sp. in noncommercial poultry and birds Source information Remarks Epidemiological unit Flocks tested Flocks positive S. Enteritidis S. Typhimurium S. Infantis S. London S. group C2 Pigeons Ducks Gallus gallus Labs. Labs. Labs. Agent id. Agent id. Agent id. Flock Flock Flock Footnote State Food and Veterinary Service; Labs. Laboratories Agent id. Agent identification Lithuania

21 Lithuania 2004 Report on trends and sources zoonoses Table Salmonella sp. in animals ( non poultry) Source information Remarks Epidemiological unit Units tested Units positive S. Enteritidis S. Typhimurium S. Dublin S. Choleraesuis S. Derby Cattle (bovine animals) Sheep Labs. Labs. Agent id. Agent id. Animal Animal 14 0 Pigs unspecified Farmed wild boars all animals Labs. Labs. Labs. Agent id. Agent id. Agent id. Animal Animal 15 0 Animal 28 0 Footnote State Food and Veterinary Service; Labs. Laboratories Agent id. Agent identification Lithuania

22 Lithuania 2004 Report on trends and sources zoonoses Salmonella in feedstuffs Table Salmonella sp. in feed material animal origin Source information Remarks Epidemiological unit Sample weight Units tested Units positive S. Enteritidis S. Typhimurium S. Agona Feed material marine animal origin Fish meal Labs. Agent id. Sample 25 g Footnote State Food and Veterinary Service; Labs. Laboratories Agent id. Agent identification Lithuania

23 Lithuania 2004 Report on trends and sources zoonoses Table Salmonella sp. in feed vegetable origin Source information Remarks Epidemiological unit Sample weight Units tested Units positive S. Enteritidis S. Typhimurium S. Agona S. Tennessee S. Dessau Feed material cereal grain origin other cereal grain derived Feed material oil seed or fruit origin other oil seeds derived Labs. Agent id. Sample 25 g 58 0 Labs. Agent id. Sample 25 g Footnote State Food and Veterinary Service; Labs Laboratories Agent id. Agent identification Lithuania

24 Table Salmonella sp. in compound feedingstuff Source information Remarks Epidemiological unit Sample weight Units tested Units positive S. Enteritidis S. Typhimurium S. group E Compound feedingstuffs for cattle NVL Agent. Final product id. Compound feedingstuffs for pigs NVL Agent. Final product id. Compound feedingstuffs for poultry (non specified) NVL Agent. Final product id. Sample 25 g 10 0 Sample 25 g Sample 25 g 22 0 Footnote NVL National Veterinary Laboratory, Agent id. Agent identification Lithuania

25 Salmonella serovars and phagetype distribution The methods collecting, isolating and testing the Salmonella isolates are described in the chapters above respectively for each animal species, foodstuffs and humans. The serotype and phagetype distributions can be used to investigate the sources the Salmonella infections in humans. Findings same serovars and phagetypes in human cases and in foodstuffs or animals may indicate that the food category or animal species in question serves as a source human infections. However as information is not available from all potential sources infections, conclusions have to be drawn with caution. Lithuania

26 Table Salmonella serovars in animals Cattle (bovine animals) Serovars Sources isolates M(*) C(*) M(*) C(*) M(*) C(*) M(*) C(*) Pigs Gallus gallus Other poultry Number isolates in the laboratory N= Number isolates serotyped N= Footnote (*) M : Monitor, C : Clinical Lithuania

27 Table Salmonella serovars in food Bovine meat Pig meat Broiler meat Other poultry Other products animal origin Bakery products Other processed food products Serovars Sources isolates M(*) C(*) M(*) C(*) M(*) C(*) M(*) C(*) M(*) C(*) M(*) C(*) M(*) C(*) Number isolates in the laboratory N= Number isolates serotyped N= Number isolates per type S. Enteritidis 1 2 S. Typhimurium 4 S. group B 1 S. group D Total typed Salmonellaisolates Footnote (*) M : Monitor, C : Clinical Lithuania

28 Table S. Enteritidis phagetypes in humans Phagetype Sources isolates M(*) C(*) Number isolates in the laboratory N= Number isolates serotyped N= humans Footnote (*) M : Monitor, C : Clinical S.Enteritidis isolates were not phagetyped Lithuania

29 Table S. Typhimurium phagetypes in humans Phagetype Sources isolates M(*) C(*) Number isolates in the laboratory N= Number isolates serotyped N= humans Footnote (*) M : Monitor, C : Clinical S.Typhimurium isolates were not phagetyped Lithuania

30 Antimicrobial resistance in Salmonella isolates Antimicrobial resistance is the ability certain microorganisms to survive or grow in the presence a given concentration antimicrobial agent that usually would kill or inhibit the microorganism species in question. Antimicrobial resistant Salmonella strains may be transferred from animals or foodstuffs to humans. Lithuania

31 Table Antimicrobial susceptibility testing S. Choleraesuis in Pigs quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Choleraesuis Pigs Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 100% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim Aminoglycosides Streptomycin % 1 Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Lithuania

32 Table Antimicrobial susceptibility testing S. Derby in Pigs quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Derby Pigs Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 100% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 0% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Lithuania

33 Table Antimicrobial susceptibility testing S. Dublin in Cattle (bovine animals) quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Dublin Cattle (bovine animals) Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 0% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 0% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Lithuania

34 Table Antimicrobial susceptibility testing S.Enteritidis in animals Isolates out a monitoring program Number isolates available in the laboratory S. Enteritidis Cattle (bovine animals) Pigs Gallus gallus Gallus gallus monitoring programme no yes Turkeys Antimicrobials: N %R N %R N %R N %R N %R Tetracycline 1 0% 14 0% Amphenicols Chloramphenicol 1 0% 14 0% Fluoroquinolones Ciprloxacin 1 0% 14 0% Quinolones Nalidixic acid 1 0% 14 0% Trimethoprim 1 0% 14 21% Aminoglycosides Streptomycin 1 0% 14 21% Gentamicin 1 100% Kanamycin 1 0% 14 0% Trimethoprim + sulfonamides 1 0% 14 21% Penicillins Ampicillin 1 0% 14 14% Number multiresistant isolates fully sensitives 8 57% resistant to % 3 21% antimicrobial resistant to 2 1 7% antimicrobials resistant to 4 antimicrobials 2 14% Lithuania

35 Table Antimicrobial susceptibility testing S. Enteritidis in Gallus gallus monitoring programme quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Enteritidis Gallus gallus monitoring programme Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R yes Tetracycline 14 0% Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 14 21% Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 14 21% Penicillins Ampicillin Lithuania

36 Table Antimicrobial susceptibility testing S. Enteritidis in Gallus gallus quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Enteritidis Gallus gallus Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 0% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 0% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Lithuania

37 Table Antimicrobial susceptibility testing S. Enteritidis in Broiler meat quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Enteritidis Broiler meat Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 9 11% Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 9 11% Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 9 0% Penicillins Ampicillin Lithuania

38 Table Antimicrobial susceptibility testing S. Enteritidis in Cream import import controls quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Enteritidis Cream import import controls Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 0% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 0% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Lithuania

39 Table Antimicrobial susceptibility testing S. Enteritidis in Prepared food, nonready to eat quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Enteritidis Prepared food, nonready to eat Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 2 0% 1 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 2 0% 2 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 2 0% 1 1 Penicillins Ampicillin Lithuania

40 Table Antimicrobial susceptibility testing S. Enteritidis in humans qualitative data S. Enteritidis humans Isolates out a no monitoring program Number isolates 1994 available in the laboratory Antimicrobials: N %R Tetracycline ,56% Amphenicols Chloramphenicol ,84% Cephalosporin Ceftazidim ,11% Fluoroquinolones Ciprloxacin ,06% Trimethoprim ,57% Aminoglycosides Gentamicin ,57% Trimethoprim + sulfonamides ,55% Penicillins Ampicillin ,37% Number multiresistant isolates fully sensitives ,91% resistant to ,88% antimicrobial resistant to ,47% antimicrobials resistant to 3 6 0,34% antimicrobials resistant to 4 5 0,29% antimicrobials resistant to >4 antimicrobials 2 0,11% Lithuania

41 Table Antimicrobial susceptibility testing S. Infantis in Gallus gallus quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Infantis Gallus gallus Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 0% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 0% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Lithuania

42 Table Antimicrobial susceptibility testing S. London in Ducks quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. London Ducks Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 3 33% Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 3 0% 2 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 3 0% Penicillins Ampicillin Lithuania

43 Table Antimicrobial susceptibility testing S. Panama in Pig meat import import controls quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Panama Pig meat import import controls Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R yes Tetracycline 1 0% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 0% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Footnote from Germany Lithuania

44 Table Antimicrobial susceptibility testing S. Schwarzengrund in Other meat quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Schwarzengrund Other meat Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 0% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 0% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 1 0% 1 Penicillins Ampicillin Lithuania

45 Table Antimicrobial susceptibility testing S. Typhimurium in Prepared food, nonready to eat ficial food or feed controls quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. Typhimurium Prepared food, nonready to eat ficial food or feed controls Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R yes Tetracycline 2 100% 2 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 2 100% 2 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides Penicillins Ampicillin % 1 1 Lithuania

46 Table Antimicrobial susceptibility testing S. Typhimurium in humans qualitative data S. Typhimurium humans Isolates out a no monitoring program Number isolates 133 available in the laboratory Antimicrobials: N %R Tetracycline 115 9,56% Cephalosporin Ceftazidim 115 0,87% Fluoroquinolones Ciprloxacin 115 0% Aminoglycosides Gentamicin 115 0,87% Trimethoprim + sulfonamides 115 4,35% Penicillins Ampicillin ,48% Number multiresistant isolates fully sensitives 83 72,17% resistant to ,0% antimicrobial resistant to 2 9 7,82% antimicrobials resistant to 3 0 antimicrobials resistant to 4 0 antimicrobials resistant to >4 0 antimicrobials Number multiresistant DT104 with penta resistance 0 Lithuania

47 Table Antimicrobial susceptibility testing Salmonella spp. in animals Isolates out a monitoring program Number isolates available in the laboratory Salmonella spp. Cattle (bovine animals) Pigs Gallus gallus Ducks Turkeys no no no no Antimicrobials: N %R N %R N %R N %R N %R Tetracycline 1 0% 2 100% 2 50% 3 33% Amphenicols Chloramphenicol 1 0% 2 50% 2 0% 3 0% Fluoroquinolones Ciprloxacin 1 0% 2 0% 2 0% 3 0% Quinolones Nalidixic acid 1 0% 2 50% 2 50% 3 0% Trimethoprim 1 0% 2 0% 2 50% 3 0% Aminoglycosides Streptomycin 1 0% 2 50% 2 50% 3 0% Gentamicin 1 0% 2 0% 2 0% 3 0% Kanamycin 1 0% 2 50% 2 50% 3 0% Trimethoprim + sulfonamides 1 0% 2 0% 2 0% 3 0% Penicillins Ampicillin 1 0% 2 0% 2 50% 3 0% Number multiresistant isolates fully sensitives 1 100% 1 50% 2 66% resistant to 1 antimicrobial 1 50% 1 33% resistant to >4 antimicrobials 1 50% 1 50% Lithuania

48 Table Antimicrobial susceptibility testing Salmonella spp. in food Salmonella spp. Broiler meat Other poultry meat Pig meat Bovine meat Other meat Prepared Prepared food, food, nonready nonready to eat to eat monitoring programme Isolates out a no yes no no no monitoring program Number isolates available in the laboratory Antimicrobials: N %R N %R N %R N %R N %R N %R N %R Tetracycline 9 11% 1 0% 1 0% Amphenicols Chloramphenicol 9 0% 1 0% 1 0% Fluoroquinolones Ciprloxacin 9 0% 1 0% 1 100% Quinolones Nalidixic acid 9 11% 1 0% 1 0% Trimethoprim 1 0% 1 0% Aminoglycosides Streptomycin 9 0% 1 0% 1 0% Gentamicin 9 0% 1 0% 1 0% Kanamycin 9 0% 1 0% 1 0% Trimethoprim + sulfonamides 9 0% 1 2% Penicillins Ampicillin 9 0% 1 0% 1 0% Number multiresistant isolates fully sensitives 7 88% 1 100% resistant to 1 antimicrobial 2 22% 1 100% Lithuania

49 Table Antimicrobial susceptibility testing Salmonella spp. in humans qualitative data Salmonella spp. humans Isolates out a no monitoring program Number isolates 2237 available in the laboratory Antimicrobials: N %R Tetracycline ,73% Amphenicols Chloramphenicol ,56% Cephalosporin Ceftazidim ,20% Fluoroquinolones Ciprloxacin ,05% Trimethoprim ,51% Aminoglycosides Gentamicin ,61% Trimethoprim + sulfonamides ,74% Penicillins Ampicillin ,95% Number multiresistant isolates fully sensitives ,94% resistant to ,38% antimicrobial resistant to ,86% antimicrobials resistant to 3 8 0,41% antimicrobials resistant to 4 6 0,31% antimicrobials resistant to >4 antimicrobials 2 0,10% Lithuania

50 Table Antimicrobial susceptibility testing S. group C in Gallus gallus monitoring programme quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. group C Gallus gallus monitoring programme Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R yes Tetracycline 2 50% 1 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 2 0% 1 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides 2 0% 1 1 Penicillins Ampicillin Lithuania

51 Table Antimicrobial susceptibility testing S. group C2 in Gallus gallus quantitative data [Diffusion method] Percentage resistant isolates (R%) and percentage isolates with the concentration (µl/ml) or zone (mm) inhibition equal to S. group C2 Gallus gallus Isolates out a monitoring program Number isolates available in the laboratory Antimicrobials: N %R no Tetracycline 1 100% 1 Amphenicols Chloramphenicol Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Trimethoprim 1 100% 1 Aminoglycosides Streptomycin Gentamicin Kanamycin Trimethoprim + sulfonamides Penicillins Ampicillin % 1 Lithuania

52 Table Breakpoints for antibiotic resistance Salmonella in Animals Test Method Used Disc diffusion Agar dilution Broth dilution Etest Standards used for testing NCCLS CASFM Subject to quality control Salmonella Standard for breakpoint Breakpoint concentration (microg/ml) Range tested concentration (microg/ml) disk content breakpoint Zone diameter (mm) Susceptible <= Intermediate Resistant > lowest highest microg Susceptible >= Intermediate Resistant <= Tetracycline Amphenicols Chloramphenicol Florfenicol Fluoroquinolones Ciprloxacin Enrloxacin Quinolones Nalidixic acid Trimethoprim Sulfonamides Sulfonamide Aminoglycosides Streptomycin Gentamicin Neomycin Kanamycin Trimethoprim , sulfonamides Cephalosporin Ceftazidim 3rd generation cephalosporins Penicillins Ampicillin Lithuania

53 Table Breakpoints for antibiotic resistance Salmonella in Food Test Method Used Disc diffusion Agar dilution Broth dilution Etest Standards used for testing NCCLS CASFM Subject to quality control Salmonella Standard for breakpoint Breakpoint concentration (microg/ml) Range tested concentration (microg/ml) disk content breakpoint Zone diameter (mm) Susceptible <= Intermediate Resistant > lowest highest microg Susceptible >= Intermediate Resistant <= Tetracycline Amphenicols Chloramphenicol Florfenicol Fluoroquinolones Ciprloxacin Enrloxacin Quinolones Nalidixic acid Trimethoprim Sulfonamides Sulfonamide 250, Aminoglycosides Streptomycin Gentamicin Neomycin Kanamycin Trimethoprim , sulfonamides Cephalosporin Ceftazidim 3rd generation cephalosporins Penicillins Ampicillin Lithuania

54 Table Breakpoints for antibiotic resistance Salmonella in Feedingstuff Test Method Used Disc diffusion Agar dilution Broth dilution Etest Standards used for testing NCCLS CASFM Subject to quality control Salmonella Standard for breakpoint Breakpoint concentration (microg/ml) Range tested concentration (microg/ml) disk content breakpoint Zone diameter (mm) Susceptible <= Intermediate Resistant > lowest highest microg Susceptible >= Intermediate Resistant <= Tetracycline Amphenicols Chloramphenicol Florfenicol Fluoroquinolones Ciprloxacin Enrloxacin Quinolones Nalidixic acid Trimethoprim Sulfonamides Sulfonamide Aminoglycosides Streptomycin Gentamicin Neomycin Kanamycin Trimethoprim , sulfonamides Cephalosporin Ceftazidim 3rd generation cephalosporins Penicillins Ampicillin Lithuania

55 Table Breakpoints for antibiotic resistance Salmonella in Humans Test Method Used Disc diffusion Agar dilution Broth dilution Etest Standards used for testing NCCLS CASFM Subject to quality control Salmonella Standard for breakpoint Breakpoint concentration (microg/ml) Range tested concentration (microg/ml) disk content breakpoint Zone diameter (mm) Susceptible <= Intermediate Resistant > lowest highest microg Susceptible >= Intermediate Resistant <= Tetracycline Amphenicols Chloramphenicol Florfenicol Fluoroquinolones Ciprloxacin Enrloxacin Quinolones Nalidixic acid Trimethoprim Sulfonamides Sulfonamide Aminoglycosides Streptomycin Gentamicin Neomycin Kanamycin Trimethoprim + sulfonamides 1.25, Cephalosporin Ceftazidim rd generation cephalosporins Penicillins Ampicillin Lithuania

56 2.2. CAMPYLOBACTERIOSIS General evaluation the national situation Campylobacteriosis in humans A. Thermophilic Campylobacter in humans Reporting system in place for the human cases Health minister's order regulates information providing system on communicable diseases and it's list. Clinicist informs territorial public healthcare institution about suspected or detected case in 12 hours by phone, in 72 hours sends an urgent report. When diagnosis is changed clinicist informs territorial public healthcare institution in 12 hours. Territorial public healthcare institutions registrate every case in standart form. In the end every month data on morbidity communicable diseases are summerised and send to national level CCDPC. CCDPC publishes monthly bulletin morbidity in infectious diseases and sends it to regional public healthcare centers, State public healthcare service, State food and veterinary service boardering countries WHO, EU communicable diseases surveillance network. Every year territorial public healthcare institutions provide annual statistical data on extended epidemiological investigations. Case definition Clinical picture compatible with campylobacteriosis, isolation Campylobacter sp. from any clinical specimen. Diagnostic/analytical methods used Methods used: Bacteriological culture Quality assurance procedures: Internal quality control sistem: a) standart media quality control procedures b) standart antimicrobial susceptibility testing control procedures External "PT" Quality Assessment Programmes. Labquality Helsinki Finland. Reference:WHO Manual for the laboratory identification and antimicrobial susceptibility testing bacterial pathogens public health concern in the developing world Notification system in place Every probable, suspected, or confirmed case is registered in personal healthcare institution according Health minister's order and is informed to territorial public healthcare institution where cases are registered. All detected cases are reported to the national level CCDPC and cases are registered in State register for communicable diseases. History the disease and/or infection in the country Lithuania

57 Campylobacteriosis started to be registrate since 1995 in Lithuania. National evaluation the recent situation, the trends and sources infection During last years incidence rate per population campylobacteriosis is increased from 0,2 in 1995 to 23,1 in Compylobacteriosis makes 5% in the structure diarrhoeal diseases. The largest morbidity is in children under 3 years. More than 50% campylobacter specimen aren't typed. Among typed campylobacter: C.jejuni make 37,3%, C. coli 3,3%, C. lari 2,1% in The results cluster investigations show that campilobacter outspreads through poultry and it's products. During last years an outbreaks aren't detected in Lithuania. Relevance as zoonotic disease As in all European countries campylobateriosis remains one the dominant zoonosis in Lithuania. Lithuania

58 Table 6.3.A Campylobacteriosis in man species/serotype distribution Cases Cases Inc Autochtone cases Autochtone Inc Imported cases Imported Inc unknown status Campylobacter C. coli C. jejuni C. upsaliensis 0 0 Campylobacter spp Footnote General 23,1 cases campylobacteriosis in 2004 per population Lithuania

59 Table 6.3.B Campylobacteriosis in man age distribution C. coli C. jejuni Campylobacter spp. Age Distribution All M F All M F All M F <1 year to 4 years(1) to 14 years(2) to 24 years(3) to 44 years(4) to 64 years(5) years and older(6) Age unknown Total : (1) : 1 to 3 years (2) : 4 to 14 years (3) : 15 to 29 years (4) : 30 to 49 years (5) : 50 to 59 years (6) : 60 years and older Lithuania

60 Table 6.3.C Campylobacteriosis in man seasonal distribution C. coli C. jejuni C. upsaliensis Campylobacter spp. Month Cases Cases Cases Cases January February March April May June July August September October November December not known Total : Lithuania

61 Lithuania 2004 Report on trends and sources zoonoses Campylobacter in foodstuffs Table 6.2 Thermophilic Campylobacter spp. in food Source information Remarks Epidemiological unit Sample weight Units tested C. coli C. lari C. upsaliensis C. jejuni Campylobacter spp. Bovine meat fresh at retail Labs. sample 25 1 meat products at processing plant Labs. sample 25 3 Pig meat fresh at retail Labs. sample 25 1 meat products at processing plant Labs. sample 25 2 Poultry meat fresh at retail Labs. sample 25 2 meat products at retail Labs. sample 25 6 Other meat meat products at processing plant Labs. sample 25 3 Dairy products readytoeat Labs. sample 25 5 Fishery products fish Table eggs Processed fruits and vegetables (1) Labs. Labs. Labs. sample 25 4 sample 25 1 sample 25 2 Lithuania

62 Lithuania 2004 Report on trends and sources zoonoses (1) : These two samples are from processed potato Labs. Laboratories Lithuania

63 Lithuania 2004 Report on trends and sources zoonoses Campylobacter in animals Table Thermophilic Campylobacter spp. in animals Source information Remarks Epidemiological unit Units tested Units positive C. jejuni C. coli C. lari C. upsaliensis Cattle (bovine animals) dairy cows Sheep Pigs Labs. Agent id. Animal Agent id. Animal 1 0 Labs. Labs. Agent id. Animal 6 0 Gallus gallus broilers at farm Labs. Agent id. Flock Other poultry Labs. Agent id. Animal 3 0 Pet animals dogs Labs. Agent id. Animal Footnote State Food and Veterinary Service; Labs. Laboratories Agent id. Agent identification Lithuania

64 Antimicrobial resistance in Campylobacter isolates Lithuania

65 Table Antimicrobial susceptibility testing Campylobacter in humans Campylobacter spp. humans Isolates out a no monitoring program Number isolates 957 available in the laboratory Antimicrobials: N %R Tetracycline 646 0,62% Fluoroquinolones Ciprloxacin ,94% Trimethoprim ,03% Macrolides Erythromycin 646 0,93% Penicillins Ampicillin ,31% Number multiresistant isolates fully sensitives ,49% resistant to ,16% antimicrobial resistant to ,80% antimicrobials resistant to ,44% antimicrobials resistant to ,11% antimicrobials resistant to >4 antimicrobials 0 Lithuania

66 Table Breakpoints used for antimicrobial susceptibility testing Campylobacter in Humans Test Method Used Disc diffusion Agar dilution Broth dilution Etest Standards used for testing NCCLS CASFM Subject to quality control Campylobacter Standard for breakpoint Tetracycline Breakpoint concentration (microg/ml) Susceptible <= Intermediate Resistant > Range tested concentration (microg/ml) disk content breakpoint Zone diameter (mm) lowest highest microg Susceptible Intermediate Resistant >= <= Fluoroquinolones Ciprloxacin Quinolones Nalidixic acid Aminoglycosides Gentamicin Macrolides Erythromycin Penicillins Ampicillin Lithuania

67 2.3. LISTERIOSIS General evaluation the national situation A. Listeriosis general evaluation National evaluation the recent situation, the trends and sources infection 26 samples ovine tested all negative Relevance the findings in animals, feedingstuffs and foodstuffs to human cases (as a source infection) 26 ovines tested all negative Lithuania

68 Listeriosis in humans A. Listeriosis in humans Reporting system in place for the human cases Health minister's order regulates information providing system on communicable diseases and it's list. Clinicist informs territorial public healthcare institution about suspected or detected case in 12 hours by phone, in 72 hours sends an urgent report. When diagnosis is changed clinicist informs territorial public healthcare institution in 12 hours. Territorial public healthcare institutions registrate every case in standart form. In the end every month data on morbidity communicable diseases are summerised and send to national level CCDPC. CCDPC publishes monthly bulletin morbidity in infectious diseases and sends it to regional public healthcare centers, State public healthcare service, State food and veterinary service boardering countries WHO, EU communicable diseases surveillance network. Every year territorial public healthcare institutions provide annual statistical data on extended epidemiological investigations. Case definition Infection caused by Listeria monocytogenes, isolation L.monocytogenes from a normaly sterile site:blood, or cerebrospinal fluid or joint, pleural or pericadial fluid. Diagnostic/analytical methods used Methods used Bacteriological culture Quality assurance procedures: Internal quality control sistem: a) standart media quality control procedures b) standart antimicrobial susceptibility testing control procedures External "PT" Quality Assessment Programmes. Labquality Helsinki Finland Reference: WHO Manual for the laboratory identification and antimicrobial susceptibility testing bacterial pathogens public health concern in the developing world Notification system in place Every probable, suspected, or confirmed case is registered in personal healthcare institution according Health minister's order and is informed to territorial public healthcare institution where cases are registered. All detected cases are reported to the national level CCDPC and cases are registered in State register for communicable diseases. National evaluation the recent situation, the trends and sources infection During last years have been registered sporadic cases listeriosis in Lithuania. Since 1998 to 2004 have been registered 8 cases listeriosis. During above mentioned period have been registered 3 fatal cases 8. For most patients the source infection isn't detected. Relevance as zoonotic disease Lithuania