ZOONOSES MONITORING. Czech Republic IN 2015 TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS

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1 ZOONOSES MONITORING Czech Republic TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS including information on foodborne outbreak, antimicrobial reitance in zoonotic and indicator bacteria and ome pathogenic microbiological agent IN 5 Czech Republic - 5

2 Czech Republic - 5 Report on trend and ource of zoonoe PREFACE Thi report i ubmitted to the European Commiion in accordance with Article 9 of Council Directive 3/99/ EC*. The information ha alo been forwarded to the European Food Safety Authority (EFSA). The report contain information on trend and ource of zoonoe and zoonotic agent in Czech Republic during the year 5. The information cover the occurrence of thee dieae and agent in animal, foodtuff and in ome cae alo in feedingtuff. In addition the report include data on antimicrobial reitance in ome zoonotic agent and indicator bacteria a well a information on epidemiological invetigation of foodborne outbreak. Complementary data on uceptible animal population in the country i alo given. The information given cover both zoonoe that are important for the public health in the whole European Union a well a zoonoe, which are relevant on the bai of the national epidemiological ituation. The report decribe the monitoring ytem in place and the prevention and control trategie applied in the country. For ome zoonoe thi monitoring i baed on legal requirement laid down by the European Union legilation, while for the other zoonoe national approache are applied. The report preent the reult of the examination carried out in the reporting year. A national evaluation of the epidemiological ituation, with pecial reference to trend and ource of zoonotic infection, i given. Whenever poible, the relevance of finding in foodtuff and animal to zoonoe cae in human i evaluated. The information covered by thi report i ued in the annual European Union Summary Report on zoonoe and antimicrobial reitance that are publihed each year by EFSA. * Directive 3/ 99/ EC of the European Parliament and of the Council of December 3 on the monitoring of zoonoe and zoonotic agent, amending Deciion 9/ / EEC and repealing Council Directive 9/ 7/ EEC, OJ L 35, 7..3, p. 3 Czech Republic - 5

3 Lit of Content TEXTFORMS ANIMAL POPULATIONS Population 3.. Information on uceptible animal population 3 DISEASE STATUS. TUBERCULOSIS, MYCOBACTERIAL DISEASES.. Mycobacterium in animal... Mycobacterium tuberculoi complex (MTC) in animal - Cattle (bovine animal) - animal ample. BRUCELLOSIS 6.. Brucella in animal 6... B. abortu in animal - Cattle (bovine animal) - animal ample 6... B. meliteni in animal - Goat - animal ample B. meliteni in animal - Sheep - animal ample 8 3 INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS 3. SALMONELLOSIS 3.. General evaluation of the national ituation 3... Salmonella - general evaluation 3.. Salmonella in foodtuff 3... Salmonella in food - Meat from bovine animal 3... Salmonella in food - Meat from broiler (Gallu gallu) Salmonella in food - Meat from pig 3... Salmonella in food - Meat from turkey 3..3 Salmonella in animal Salmonella pp., unpecified in animal - Gallu gallu (fowl) - broiler Salmonella pp., unpecified in animal - Gallu gallu (fowl) - breeding flock, unpecified Salmonella pp., unpecified in Turkey - breeding flock and meat production flock 9 3. CAMPYLOBACTERIOSIS 3.. General evaluation of the national ituation 3... Thermophilic Campylobacter pp., unpecified - general evaluation 3.. Campylobacter in animal 3... Thermophilic Campylobacter pp., unpecified in animal - All animal - Slaughterhoue - animal ample - caecum 3.3 LISTERIOSIS General evaluation of the national ituation Literia - general evaluation Literia in foodtuff L. monocytogene in food - Other food - Retail - Surveillance - Official ampling - Objective ampling L. monocytogene in food - Other food - Proceing plant - food ample - Surveillance - Official ampling - Objective ampling L. monocytogene in food - Other food - Retail - food ample - Surveillance - Official ampling - Objective ampling 5 3. TRICHINELLOSIS Trichinella in animal Trichinella in animal - Meat from pig - food ample RABIES General evaluation of the national ituation Lyaviru (rabie) - general evaluation Lyaviru (rabie) in animal Lyaviru (rabie) in animal - Dog - animal ample Q-FEVER General evaluation of the national ituation Coxiella (Q-fever) - general evaluation VTEC General evaluation of the national ituation Verotoxigenic E. coli (VTEC) - general evaluation Echerichia coli in foodtuff Verotoxigenic E. coli (VTEC) in food - Meat from bovine animal - carcae - Slaughterhoue - food ample - carcae wab - Monitoring - Official ampling - Objective ampling Verotoxigenic E. coli (VTEC) in food - Meat from pig - carcae - Slaughterhoue - food ample - carcae wab - Monitoring - Official ampling - Objective ampling 3 ANTIROBIAL RESISTANCE INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS. CAMPYLOBACTERIOSIS Campylobacter in foodtuff 3... Antimicrobial reitance in Campylobacter Gallu gallu (fowl) 3... Antimicrobial reitance in Campylobacter Pig 3.. Campylobacter in animal 3... Antimicrobial reitance in Thermophilic Campylobacter pp., unpecified All animal 3. ESCHERICHIA COLI, NON-PATHOGENIC 3.. Echerichia coli, non-pathogenic in foodtuff 3... Antimicrobial reitance in Echerichia coli, non-pathogenic Gallu gallu (fowl) 3... Antimicrobial reitance in Echerichia coli, non-pathogenic Meat from bovine animal...3 Antimicrobial reitance in Echerichia coli, non-pathogenic Meat from broiler (Gallu gallu) Antimicrobial reitance in Echerichia coli, non-pathogenic Meat from pig Antimicrobial reitance in Echerichia coli, non-pathogenic Pig 35 5 INFORMATION ON SPECIFIC ROBIOLOGICAL AGENTS CRONOBACTER Cronobacter in foodtuff Cronobacter in food - Dairy product (excluding cheee) - milk powder and whey powder - Proceing plant - food ample HISTAMINE Hitamine in foodtuff Hitamine in food Hitamine in food - Fih - Fihery product from fih pecie aociated with a high amount of hitidine - not enzyme maturated - Proceing plant - food ample - Surveillance - Official ampling - Objecti Hitamine in food - Fih - Fihery product from fih pecie aociated with a high amount of hitidine - not enzyme maturated - Wholeale - food ample - Surveillance - Official ampling - Objective am STAPHYLOCOCCAL ENTEROTOXINS Staphylococcal enterotoxin in foodtuff Staphylococcal enterotoxin in food Staphylococcal enterotoxin in food - Cheee made from cow' milk - Proceing plant - food ample 6 FOODBORNE OUTBREAKS 6. Outbreak 6.. Foodborne outbreak ANIMAL POPULATION TABLES DISEASE STATUS TABLES FOR BRUCELLA Bovine brucelloi in countrie and region that do not receive Community co-financing for eradication programme Ovine or Caprine brucelloi in countrie and region that do not receive Community co-financing for eradication programme 6 DISEASE STATUS TABLES FOR MYCOBACTERIUM 7 Bovine tuberculoi in countrie and region that do not receive Community co-financing for eradication programme 7 PREVALENCE TABLES 8 CAMPYLOBACTER 8 animal 8 food 9 COXIELLA 5 animal 5 CRONOBACTER 5 food 5 ESCHERICHIA COLI 5 food 5 FLAVIVIRUS 53 animal 53 HISTAMINE 5 food 5 5 5

4 LISTERIA 55 food 55 LYSSAVIRUS 6 animal 6 SALMONELLA animal 6 6 food 63 STAPHYLOCOCCAL ENTEROTOXINS 7 food 7 TRICHINELLA animal 7 7 FOODBORNE OUTBREAKS TABLES 73 AMR TABLES FOR CAMPYLOBACTER 76 Campylobacter coli 76 Pig - fattening pig - Slaughterhoue - Monitoring - active - Official ampling - AMR MON 76 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - active - Official ampling - AMR MON 77 Campylobacter jejuni 78 Pig - fattening pig - Slaughterhoue - Monitoring - active - Official ampling - AMR MON 78 Gallu gallu (fowl) - broiler - Slaughterhoue - Monitoring - active - Official ampling - AMR MON 79 AMR TABLES FOR SALMONELLA 8 Salmonella,,[5],:i:- - DT 93 8 Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 8 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 8 Meat from pig - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 8 Salmonella,,[5],:i:- - DT 8 83 Meat from bovine animal - carcae - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 83 Salmonella,,[5],:i:- - DT U Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON Salmonella,:b:- 85 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Indutry ampling - AMR MON 85 Salmonella 6,7:-:,5 86 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON Salmonella Agona Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON Meat from pig - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 88 Salmonella Anatum 89 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 89 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - AMR MON 9 Salmonella Bardo 9 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 9 Salmonella Bareilly 9 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 9 Salmonella Derby 93 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 93 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 9 Salmonella enterica ubp. enterica rough 95 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 95 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 96 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - AMR MON 97 Meat from pig - carcae - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 98 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 99 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON Salmonella Enteritidi b Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON Geee - meat production flock - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 3 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Indutry ampling - AMR MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 5 Salmonella Enteritidi 6c 6 Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 6 Salmonella Enteritidi PT 3a 7 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON Salmonella Enteritidi PT Gallu gallu (fowl) - laying hen - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON Salmonella Enteritidi PT a 9 Meat from broiler (Gallu gallu) - carcae - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 9 Salmonella Enteritidi PT Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON Salmonella Enteritidi PT c Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - AMR MON 3 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 5 6 Salmonella Enteritidi PT 3 7 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 7 Salmonella Enteritidi PT 8 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 8 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 9 Salmonella Enteritidi PT b Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON Gallu gallu (fowl) - laying hen - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON Salmonella Enteritidi PT 6a 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 3 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - AMR MON Salmonella Enteritidi PT 7 5 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 5 Salmonella Enteritidi PT 8 6 Meat from turkey - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 6 Meat from bovine animal - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 7 Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 8 Cattle (bovine animal) - meat production animal - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 9 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 3 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - AMR MON 3 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 3 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Indutry ampling - AMR MON 33 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official ampling - AMR MON 3 Gallu gallu (fowl) - laying hen - Farm - Control and eradication programme - Official ampling - AMR MON 35 Gallu gallu (fowl) - laying hen - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 36 Gallu gallu (fowl) - broiler - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 37 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 38 Salmonella Enteritidi RDNC 39 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 39 Salmonella Enteritidi U Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Official ampling - AMR MON Salmonella Gallinarum biovar Pullorum

5 Gallu gallu (fowl) - laying hen - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON Salmonella Give 3 Duck - meat production flock - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 3 Duck - meat production flock - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 5 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - AMR MON 6 Salmonella Indiana 7 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 7 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 8 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - AMR MON 9 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 5 Salmonella Infanti 5 Pet food - dog nack (pig ear, chewing bone) - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 5 Meat, mixed meat - meat product - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 5 Meat from bovine animal - carcae - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 53 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 5 Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 55 Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON pnl 58 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 59 Meat from broiler (Gallu gallu) - meat product - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 6 Meat from broiler (Gallu gallu) - meat product - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 6 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 6 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 63 Meat from pig - carcae - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 6 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON pnl 65 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 66 Meat from pig - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 67 Meat from broiler (Gallu gallu) - carcae - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 68 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 69 Salmonella Kentucky 7 Meat from turkey - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 7 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 7 Gallu gallu (fowl) - parent breeding flock for broiler production line - Farm - Control and eradication programme - Official ampling - AMR MON pnl 7 Gallu gallu (fowl) - parent breeding flock for broiler production line - Farm - Control and eradication programme - Official ampling - AMR MON 73 Turkey - fattening flock - Farm - Control and eradication programme - Indutry ampling - AMR MON 7 Salmonella Kottbu 75 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 75 Salmonella London 76 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 76 Salmonella Mbandaka 77 Meat from broiler (Gallu gallu) - mechanically eparated meat (MSM) - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 77 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 78 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 79 Salmonella Montevideo 8 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 8 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 8 Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 8 Salmonella Newport 83 Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 83 Salmonella Ohio 8 Sheep - animal under year (lamb) - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON Meat from broiler (Gallu gallu) - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 86 Meat from broiler (Gallu gallu) - freh - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 87 Gallu gallu (fowl) - parent breeding flock for broiler production line - Farm - Control and eradication programme - Indutry ampling - AMR MON 88 Gallu gallu (fowl) - parent breeding flock for broiler production line - Farm - Control and eradication programme - Official ampling - AMR MON Meat from broiler (Gallu gallu) - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON Salmonella Rien Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON Salmonella Saintpaul Meat from turkey - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 9 Salmonella Schwarzengrund 93 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 93 Salmonella Stanley 9 Meat from turkey - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 9 Salmonella Stanleyville 95 Meat, mixed meat - meat product - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 95 Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 96 Meat from bovine animal - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON 97 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 98 Salmonella Tenneee 99 Gallu gallu (fowl) - broiler - Farm - Control and eradication programme - Indutry ampling - AMR MON 99 Salmonella Tumodi Meat from pig - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON pnl Meat from pig - meat preparation - Proceing plant - Surveillance - Official ampling - OTHER AMR MON Salmonella Typhimurium DT Pet food - dog nack (pig ear, chewing bone) - Proceing plant - Surveillance - Official ampling - OTHER AMR MON Meat from bovine animal - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 3 Cattle (bovine animal) - calve (under year) - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 5 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 6 Salmonella Typhimurium DT 7 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 7 Salmonella Typhimurium DT 8 Pig - fattening pig - Farm - Clinical invetigation - Indutry ampling - OTHER AMR MON 8 Salmonella Typhimurium DT 93 9 Meat from pig - carcae - Slaughterhoue - Monitoring - Official ampling - AMR MON 9 AMR TABLES FOR ESCHERICHIA COLI Echerichia coli, non-pathogenic, unpecified Pig - fattening pig - Slaughterhoue - Monitoring - Official ampling - AMR MON pnl Pig - fattening pig - Slaughterhoue - Monitoring - Official ampling - AMR MON Pig - fattening pig - Slaughterhoue - Monitoring - Official ampling - ESBL MON pnl Pig - fattening pig - Slaughterhoue - Monitoring - Official ampling - ESBL MON 3 Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON pnl Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON 5 Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 6 Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON 7 Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 8 Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON 9 Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON pnl Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON pnl Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON 3 Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON pnl Meat from bovine animal - freh - Retail - Monitoring - Official ampling - ESBL MON 5 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 6 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON

6 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 8 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON 9 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 3 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON 3 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 3 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON 33 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON pnl 3 Meat from pig - freh - Retail - Monitoring - Official ampling - ESBL MON 35 OTHER AMR TABLES 36 ESBL 37

7 ANIMAL POPULATIONS The relevance of the finding on zoonoe and zoonotic agent ha to be related to the ize and nature of the animal population in the country. Population.. Information on uceptible animal population Source of information Information on uceptible animal population come from the Central Databae of Animal in the Czech Republic which i performed in accordance with Breeding Act No. 5/ Coll., a amended and from the State Veterinary Adminitration databae. Date the figure relate to and the content of the figure Number of animal and holding related to National evaluation of the number of uceptible population and trend in thee figure Number of cattle holding were 739 in total related to and N. of animal were in total. It mean that cattle population trend i imilar in comparion with year. N. of heep and goat (holding and animal) have increaing trend in the year 5. N. of pig holding i imilar a in the year, but N. of animal have decreaed trend. In hore population i evident increing trend. Czech Republic - 5 3

8 DISEASE STATUS. TUBERCULOSIS, MYCOBACTERIAL DISEASES.. Mycobacterium in animal... Mycobacterium tuberculoi complex (MTC) in animal - Cattle (bovine animal) - animal ample Statu a officially free of bovine tuberculoi during the reporting year The entire country free The Czech Republic i free of Bovine tuberculoi caued by M. bovi ince 967 on the national level and from i declared a officially free in accordance with EU legilation on the bae of Commiion Deciion /3/EC. Free region The whole territory of the Czech Republic i declared a a officially free of tuberculoi (M. bovi) in relation to bovine herd. Additional information During the reporting year 5 there wa no occurrence and/or outbreak of bovine tuberculoi caued by Mycobacterium bovi in bovine animal. Monitoring ytem Sampling trategy The ampling trategy and monitoring ytem i in accordance with Directive 6/3/EEC a amended. Frequency of the ampling Tuberculoi Alergenodiagnoi imple kin tet (antigen "Bovitubal" M. bovi 8 IU). Data of the lat kin tet mut be checked prior to kin tet in order to oberve pecified time period between individual examination. a) Examination of % of the female bovine animal aged over month in each region (examination of all animal, maximum animal per holding); b) Animal imported from third countrie (excluding laughter animal) examination of female animal and breeding bull over 6 week of age. The examination mut be carried out a oon a poible after arrival of animal to the place of detination with repect to eventual previou tuberculin tet; c) Animal moved from Member State not having tatu of bovine tuberculoi officially free country or region (excluding laughter animal) - examination of female animal and breeding bull over 6 week of age. The examination mut be carried out a oon a poible after arrival of animal to the place of detination with repect to eventual previou tuberculin tet; Breeding bull in BBRH examination within 8 day prior to baic election; e) Breeding bull prior to admiion to emen collection centre examination in accordance with Annex to Decree No. 38/3 Coll; f) Breeding bull in emen collection centre x per year examination in accordance with Annex to Decree No. 38/3. Coll. Type of pecimen taken kin tet Method of ampling (decription of ampling technique) Czech Republic - 5

9 The place of antigen application i ituated at the border of the anterior and middle third of the neck. The kin mut be without pathological change, equally thick with the poibility of an eay cutaneou drape formation. The place of tuberculin adminitration i perfectly cut and cleaned. The cutaneou drape i formed with the thumb and the point finger and it thickne i after cutimetre meauring recorded. The doage of. ml of tuberculin i applicated by mean of a hort terile needle, bevel edge outward, with graduated yringe charged with tuberculin, inerted obliquely into the deepet layer of the kin. The right reaction after intradermal adminitration - the papula formation in the place of allergen inoculation - mut be detected by palpation. If the tuberculin wa not adminitered intradermally, it i poible to repeat the adminitration in the ame place in the precribed doage. If the kin i injured during cutting or if kin change are determined before tuberculin adminitration, it i neceary to inoculate tuberculin on another place of the ame neck ide. The origin place i cancelled with the hair cut. Cae definition Negative reaction: If there i apparent only bordered welling with the cutaneou drape trengthening of max. mm without clinical ymptom a diffuion or large welling, exudation, necroi, painfulne or inflammation reaction of the correponding lymphatic veel or lymphatic node. Dubiou reaction: If there i apparent no clinical ymptom tated in item a) but the cutaneou drape trengthening i higher than mm but lower than mm. Poitive reaction: If there are apparent clinical ymptom tated in item a) or the cutaneou drape in the place of application i thicker by mm or more. Diagnotic/analytical method ued Simple kin tet ha been performed with tuberculin BOVITUBAL 8 IU/ml (Bioveta, CZ) which contain tuberculin protein from Mycobacterium bovi (train AN 5). The doe for one animal i,ml. The diagnotic method i in accordance with recommendation OIE. Vaccination policy Vaccination i trictly prohibited. Other preventive meaure than vaccination in place All laughtered bovine animal are under veterinary control. The official pot mortem veterinary examination i carried out in laughterhoue by the official veterinarian in accordance with EU legilation. Control program/mechanim The control program/trategie in place The control of bovine tuberculoi i performed in accordance with Directive 6/3/EC a amended. Meaure in cae of the poitive finding or ingle cae In the cae of poitive reult of examination the appropriate Regional Veterinary Adminitration iue extraordinary veterinary meaure in accordance with Veterinary Act (CZ legilation) and EU legilation. Notification ytem in place Notification ytem i laid down by the Act No. 66/999 Coll, on veterinary care and amending certain related law (Veterinary Act), a amended. Reult of the invetigation If the reult of invetigation i poitive, the peron reponible for the laboratory carrying out the examination, the peron carrying out the examination or the owner of the animal hall notify the reult to the competent authority. National evaluation of the recent ituation, the trend and ource of infection Czech Republic - 5 5

10 In the Czech Republic bovine tuberculoi wa uppreed in frame of the nationwide anitation program ( ) on October 968. The pot-eradication period ( ) wa characterized by the extinction of reervoir ource. Currently only the poradic cae of the bovine tuberculoi incidence have been recorded. In 98, 987 to 99, 993 and 996 any bovine tuberculoi incidence wa not found. Thereat in other year, from 98 to 995, at the mot three outbreak of tuberculoi ever appeared in cattle. The participation of the infected animal in individual tock wa very low and never exceeded 5 to % of animal. In 97 to 995 the Mycobacterium bovi infection wa alo diagnoed in other 9 animal (zoo, wild live, backyar and in ten milk pecimen. By coure of the O.I.E. (International Animal Health Code,chapter 3..3.) definition the territory of the Czech Republic i free from bovine tuberculoi (the prevalence up to,% of infected cattle tock). Relevance of the finding in animal to finding in foodtuff and to human cae (a a ource of infection) There i an paradox ituation becaue human become rik for animal, mainly worker from eater third countrie can be ource of infection for animal. Additional information In the framework of the health control paid by the tate, bovine tuberculoi i currently monitored in the CR a follow: ingle tuberculin tet, imultaneou tuberculin tet, laboratory examination (ection, hitological invetigation and bacteriological invetigation), erological invetigation.. BRUCELLOSIS.. Brucella in animal... B. abortu in animal - Cattle (bovine animal) - animal ample Statu a officially free of bovine brucelloi during the reporting year The entire country free The Czech Republic i free of bovine brucelloi ince 96 at the natinal level and ince i declared a officially free of bovine bruceloi according to Commiion Deciion /3/EC. Additional information During the reporting year 5 there wa no occurrence and/or outbreak of bovine brucelloi in the whole territory of the Czech Republic. Monitoring ytem Sampling trategy Sample are taken from:. All breeding bull, all abortion animal - blood ample.. % of cattle - all female animal from age month, all female - maximum head per holding - blood ample. 3. Abortion foetue in indicated caae.. Imported animal from third countrie and animal coming from EU tate/region not officially free from bruceloi (except for laughter animal) - teting of female animal older than month of age and breeding bull. Frequency of the ampling Czech Republic - 5 6

11 Sampling cheme: ) Brucelloi erological examination(rbt or ELISA): a) breeding bull in breeding bull rearing houe - examination within 8 day prior to baic election; b) breeding bull prior to admiion to emen collection centre - examination in accordance with Annex to Decree No. 38/3 Coll.; c) breeding bull in emen collection centre x per year examination in accordance with Annex to Decree No. 38/3 Coll.; all aborting cow are erologicaly teted for Brucelloi after abortion. ) Brucelloi erological examination (ELISA): a) animal imported from third countrie (excluding laughter animal) examination of female animal over month of age and breeding bull. The examination mut be carried out at mot month after arrival of animal to the place of detination; b)animal moved from Member State/region not officially free from bovine brucelloi (excluding laughter animal) - examination of female animal over month of age and breeding bull. The examination mut be carried out at mot month after arrival of animal to the place of detination; c) Blood ample from % of all female animal older moth x per year are teted for Brucelloi (all female animal, maximum animal per holding in each region). 3) Abortion and amnia examination in indicated cae. Type of pecimen taken blood, abortion foetu Diagnotic/analytical method ued The diagnotic method are ued in accordance with Directive 6/3/EEC, Regulation /6/EEC. RBT, Complement fixation tet, ELISA, low agglutination. Vaccination policy Vaccination i trictly prohibited. Other preventive meaure than vaccination in place Control of animal movement between region and control of imported animal.... B. meliteni in animal - Goat - animal ample Statu a officially free of caprine brucelloi during the reporting year The entire country free The whole teritory of the Czech Republic i officially free of Sheep and goat bruceloi in accordance with Commiion Deciion No. 3//EC. Monitoring ytem Sampling trategy The ampling trategy wa done by State Veterinary Adminitration in Methodology of control of animal healt which i laid down in accordance with Veterinary Act No. 66/999 Coll. a amended. Frequency of the ampling Caprine brucelloi (B. meliteni)-: a) Aborting goat erological examination after abortion; b) Licened breeding buck examination x per year in accordance with Annex 9 to Decree No. 38/3 Coll; c) holding (herd) producing young breeding animal where the teting i carried out annually. Repreentative number of animal hall include:. all non-catrated male animal over 6 month of age;. 5% of female animal over month of age of at leat 5 female animal (all animal teted in holding containing le than 5 animal); Type of pecimen taken Blood Czech Republic - 5 7

12 Method of ampling (decription of ampling technique) The method of ampling i in according with Annex of the Council Deciion 9//EEC Diagnotic/analytical method ued The diagnotic method were ued in accordance with Directive 6/3/EEC and Regulation /6/EEC. RBT, CFT, ELISA and low agglutination. Vaccination policy Vacination i trictly prohibited. Other preventive meaure than vaccination in place Control of animal movement between region and control of imported animal. National evaluation of the recent ituation, the trend and ource of infection The dieae ha never been recorded in the Czech Republic....3 B. meliteni in animal - Sheep - animal ample Statu a officially free of ovine brucelloi during the reporting year The entire country free The Czech Republic i officialy free of ovine bruceloi in accordance with 3//EC. Free region All region in the Czech republic are free of ovine bruceloi (B. meliteni) and the dieae ha never been found in the Czech Republic. Monitoring ytem Sampling trategy The ampling trategy wa done by State Veterinary Adminitration in Methodology of control of animal health which i laid down in accordance with Veterinary Act No. 66/999 Coll. a amended. Frequency of the ampling Ovine and caprine brucelloi (B. meliteni): a) Licened breeding ram examination x per year in accordance with Annex 9 to Decree No. 38/3 Coll; b) Holding (herd) producing young breeding animal where the teting i carried out annually. Repreentative number of animal hall include:. all non-catrated male animal over 6 month of age;. 5% of female animal over month of age of at leat 5 female animal (all animal teted in holding containing le than 5 animal); c) Aborting ewe are erologicaly teted once after abortion; Abortion or amnia examination in indicated cae. Type of pecimen taken blood and foetue Czech Republic - 5 8

13 Method of ampling (decription of ampling technique) The method of ampling and teting i in according with Annex of the Council Deciion 9//EEC Vaccination policy Vaccination i trictly prohibited. Other preventive meaure than vaccination in place Control of animal movement between region and control of imported animal. Czech Republic - 5 9

14 3 INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS Zoonoe are dieae or infection, which are naturally tranmiible directly or indirectly between animal and human. Foodtuff erve often a vehicle of zoonotic infection. Zoonotic agent cover virue, bacteria, fungi, paraite or other biological entitie that are likely to caue zoonoe. 3. SALMONELLOSIS 3.. General evaluation of the national ituation 3... Salmonella - general evaluation Hitory of the dieae and/or infection in the country The monitoring and control programme for Salmonella are carried out in the whole food chain. To thi programme are involved three intitution which are in charge for food afety and public health protection. Czech Agricultre and Food Inpection Authority and State Veterinary Adminitration have been etablihed by Minitry of Agriculture and National Intitute of Public Health ha been etablih by Minitry of Health. The Salmonelloi i notifiable dieae in both in human and animal population and the obligation for notification i laied down in the legilation. 3.. Salmonella in foodtuff 3... Salmonella in food - Meat from bovine animal Control program/mechanim Recent action taken to control the zoonoe SVA, NIPH and CAFIA carry out monitoring and control program in the whole food chain and take appropriate meaure according to the legilation in force to enure afe foodtuff. Meaure in cae of the poitive finding or ingle cae In the cae of poitive reult of the invetigation the competent authority take meaure to prevent preading of the infection to the food chain Salmonella in food - Meat from broiler (Gallu gallu) Monitoring ytem Type of pecimen taken Czech Republic - 5 At meat proceing plant

15 Final product. At retail Freh meat. Final product. Method of ampling (decription of ampling technique) At meat proceing plant The ample - meat product (final product), were placed aeptically into a ample container and tranfer to the laboratory. Definition of poitive finding At laughterhoue and cutting plant Preence of almonella in 5 g of ample. At meat proceing plant Preence of almonella in 5 g of ample. Diagnotic/analytical method ued At laughterhoue and cutting plant Bacteriological method: ISO 6579:. At meat proceing plant Bacteriological method: ISO 6579:. At retail Bacteriological method: ISO 6579:. Preventive meaure in place Creation and control of HACCP and GHP ytem. Control program/mechanim The control program/trategie in place The competent authority take meaure according to the legilation in force and defined cae are reported into the Rapid Alert Sytem for Food and Feed. Recent action taken to control the zoonoe Czech Republic - 5

16 SVA, NIPH and CAFIA carry out monitoring and control program in the whole food chain and take appropriate meaure according to the legilation in force to enure afe foodtuff. Meaure in cae of the poitive finding or ingle cae In the cae of poitive reult of the invetigation the competent authority take meaure to prevent preading of the infection to the food chain Salmonella in food - Meat from pig Control program/mechanim Recent action taken to control the zoonoe SVA, NIPH and CAFIA carry out monitoring and control program in the whole food chain and take appropriate meaure according to the legilation in force to enure afe foodtuff. Meaure in cae of the poitive finding or ingle cae In the cae of poitive reult of the invetigation the competent authority take meaure to prevent preading of the infection to the food chain Salmonella in food - Meat from turkey Monitoring ytem Sampling trategy At meat proceing plant The ample were taken in the ordinary urveillance. The final product are ampled at retail level by CAFIA according to Commiion Regulation (EC) No 73/5 of 5 November 5 on microbiological criteria for foodtuff. There i no official National Programme for the Monitoring of Salmonella pp. at retail. Sample are collected from all region of the Czech Republic within the year a part of an official ampling, i.e. it i performed by inpector from the Regional Inpectorate, the ample are then analyed at the accredited laboratorie. Frequency of the ampling At meat proceing plant Sampling ditributed evenly throughout the year. Type of pecimen taken At meat proceing plant Final product. Method of ampling (decription of ampling technique) Czech Republic - 5

17 At meat proceing plant The ample - one piece of final product mut be placed aeptically into a ample container and tranfer to the laboratory. Definition of poitive finding At laughterhoue and cutting plant Preence of almonella in 5 g of ample. At meat proceing plant Preence of almonella in 5 g of ample. Diagnotic/analytical method ued At laughterhoue and cutting plant Bacteriological method: ISO 6579:. At meat proceing plant Bacteriological method: ISO 6579:. Preventive meaure in place Creation and control of HACCP and GHP ytem. Control program/mechanim The control program/trategie in place The competent authority take meaure according to the legilation in force and defined cae are reported into the Rapid Alert Sytem for food and feed. Recent action taken to control the zoonoe SVA, NIPH and CAFIA carry out monitoring and control program in the whole food chain and take appropriate meaure according to the legilation in force to enure afe foodtuff. Meaure in cae of the poitive finding or ingle cae In the cae of poitive reult of the invetigation the competent authority take meaure to prevent preading of the infection to the food chain Salmonella in animal Czech Republic - 5 3

18 3..3. Salmonella pp., unpecified in animal - Gallu gallu (fowl) - broiler Monitoring ytem Sampling trategy Broiler flock The programme cover all flock of broiler. It doe not apply to flock for private dometic ue. The ampling frame and frequency of ampling i in compliance with Commiion Regulation (EU) No /. A farmer ample flock of broiler within three week before laughter according to etablihed chedule. It i the environmental ample - boot wab. The tet reult have to be available for CA/farmer/laughterhoue operator before the broiler tranportation to the laughterhoue. The ampling hall be done by trained peron, i.e. by operator, private veterinarian, or other determined by farmer. The relevant RVA organize a training for thee people. Official ampling within 3 week before laughtering hall be performed in one flock of broiler per year on % of holding compriing at leat 5, bird, which i in compliance with minimum requirment of Regulation (EU) No /. Furthermore, official ampling hall be performed each time the RVA conider it neceary. Official ampling hall be done only by official veterinarian from the relevant RVA. Frequency of the ampling Broiler flock: Before laughter at farm A farmer ample flock of broiler within three week before laughter according to etablihed chedule. The tet reult have to be available for CA/farmer/laughterhoue operator before the broiler tranportation to the laughterhoue. Type of pecimen taken Broiler flock: Before laughter at farm It i the environmental ample - boot wab. Method of ampling (decription of ampling technique) Broiler flock: Before laughter at farm Sampling protocol i in compliance with Commiion Regulation (EU) No /. Two pair of boot wab hall be taken for ampling. The boot wab from one flock have to be pooled into one ample. Before putting on the boot wab, their urface hall be moitened by the application of maximum recovery diluent (MRD:.8 % odium chloride,. % peptone in terile deionied water), or terile water or any other diluent approved by the National Reference Laboratory, the SVI in Prague. The ue of water containing antimicrobial or additional diinfectant i prohibited. The recommended way to moiten boot wab i to pour the liquid inide before putting them on. Alternatively, boot wab or ock may be autoclaved with diluent within autoclave bag or jar before ue. Diluent may alo be applied after boot are put on uing a pray or wah bottle. All ection in a houe are repreented in the ample. Each pair hould cover about 5 % of the houe area. The boot/ock wab hall be carefully removed o a not to dilodge adherent material. Boot wab may be inverted to retain material. They hall be placed in a bag or pot and labelled. The RVA train the operator and/or other peron deignated by farmer to guarantee the correct application of the ampling protocol. Cae definition Broiler flock: Before laughter at farm Czech Republic - 5

19 The broiler flock i conidered to be Salmonella Enteritidi / Salmonella Typhimurium (SE/ST) infected, when the poitive SE/ST laboratory tet reult of official ample/fbo ample. (other than vaccine almonella train) Diagnotic/analytical method ued Broiler flock: Before laughter at farm The laboratorie in charge of Salmonella pp. teting within the programme hall be nominated in accordance with Article of Regulation (EC) No 6/3 and are under the uperviion of the State Veterinary Adminitration. The laboratorie carry out teting of official and FBO ample. They are all accredited by ISO 75. The frame of activitie include detection of Salmonella in animal faece, tiue, environmental ample, feed and water, erotyping. The method i a modification of ISO 6579 (), where a emi-olid medium (MSRV) i ued a the ingle elective enrichment medium. Method ued in the teting are in accordance with Annex of Commiion Regulation (EU) No /. FBO and official ampling hall be teted in laboratorie of the State Veterinary Intitute (hereinafter referred to a the SVI); activitie of the laboratorie hall be coordinated by the National Reference Laboratory (hereinafter referred to a the NRL). The laboratory information ytem i connected to the Information Sytem of the SVA CR. See the laboratorie: SVI Prague NRL, SVI Jihlava, SVI Olomouc Vaccination policy Broiler flock Vaccination of broiler flock i voluntary. Control program/mechanim The control program/trategie in place Broiler flock The programme i implemented on the whole territory of the Czech Republic. The Regional Veterinary Adminitration (RVA) are in charge of programme performance. FBO ampling i done by trained operator or private veterinarian. Official veterinarian do official ampling. All ample are teted only in laboratorie of the State Veterinary Intitute (SVI) under the uperviion of the State Veterinary Adminitration (SVA). The external agencie or laboratorie are not involved in the programme. The laboratorie end the reult of all ample teted in the framework of thi programme to the relevant Regional Veterinary Adminitration (RVA). The copy of document with thee reult i end to a farmer or a private veterinarian. The laboratorie end the complete tet reult including the erotyping. The poitive reult for Salmonella pp. are end alo to SVA. The RVA ha to enter the data about almonella ampling and it reult into the Information Sytem. The SVI in Prague, the National Reference Laboratory for Salmonella, ummarize data from all laboratorie in the Czech Republic monthly. The ummarized data are provided to the SVA CR monthly. The programme ha been approved by the Commiion. It i baed on EU and national legilation. The laughterhoue need to be provided with all information about flock, which i going to be laughtered. Thi information contain tet reult within the National Salmonella Control Programme for broiler. A farmer ha to provide FCI with the information about the reult of Salmonella teting in the cae of a poitive/negative reult for all Salmonella erotype. Meaure in cae of the poitive finding or ingle cae Broiler flock: Before laughter at farm The laughterhoue need to be provided with all information about flock, which i going to be laughtered. Thi information contain tet reult within the National Salmonella Control Programme for broiler. A farmer ha to provide FCI with the information about the reult of Salmonella teting in the cae of a poitive/negative reult for all Salmonella erotype. The laughterhoue operating intruction contain meaure taken in the cae of laughtering almonella poitive flock. The mechanical cleaning, diinection, diinfetation and rat control i performed when depopulation of an infected flock. The afe elimination of faece or litter i done. Farmer hall take wab ample and end for laboratory teting of diinfection efficacy. New broiler flock might be introduced to the houe once the efficacy of diinfection reult i known. Official ampling for diinfection efficacy teting i performed, if RVA conider it neceary. Diinfection efficacy ample contain 6 wab. Thee ix wab are collected from each houe after mechanical cleaning and diinfection. Sampling ha to be carried out after expoure time of the diinfectant from dry urface no later than 7 hour after diinfection. The wiping area for one wab ample i cm. The interpretation of the final diinfection efficiency i calculated a the arithmetic average of the value of CFU / cm detected in individual mear. Diinfection i conidered effective if the average i le than 5 x 5 CFU per cm. Czech Republic - 5 5

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