CURRICULUM VITAE. Johnson Nduhiu Gitahi

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1 CURRICULUM VITAE Johnson Nduhiu Gitahi Contacts: Phone Postal address P. O. Box , Nairobi, Kenya Personal Details: Date of Birth November 1971 Nationality Kenyan Marital Status Married with three children EDUCATION: PhD Immunology; Kenyatta University (2014) Ongoing Molecular characterization and serotyping of enteric pathogens for validation of peepoo sanitization along handling chain at urban informal settlement, Kenya. Kenya Institute of Management: Diploma in Project Management Kenyatta University: Master of Science Immunology (Molecular Bio.) (Cellular, Clinical, Reproductive, Molecular and Parasitic Immunology, Immunochemistry, Immunotechniques, Immunohaematology, and Statistics,) Kenya Polytechnic: Higher Diploma in Medical Laboratory Science (Immunology, Bacteriology, and Virology) Kenya Christian Industrial Training Institute: Technical Certificate in Microcomputer Electronics. (Basic, advanced & digital electronics, Computer application & operation software.) Kenya polytechnic: Diploma in medical laboratory science (Immunology, Microbiology, Parasitology, Haematology, Histology and Physiology) Kimathi High School: Kenya Certificate of Secondary Education Curriculum Vitae: Nduhiu Gitahi : 2015 Page 1

2 SHORT COURSES: 1. Certificate in Training of Trainers in Laboratory Aspects of Antiretroviral Therapy, February Held at Ethiopian Health and Nutrition Research Institute, National HIV Laboratory. 2. Certificate in Bioinformatics & Genomics. November 25-29, Held at University of Nairobi, Center for Biotechnology and Bioinformatics, sponsored by The National Science Foundation, The Fulbright Foundation, Lewis & Clark and Reed College. 3. Certificate in performance enhancement. July 28 th -29 th, Held at University of Nairobi, by Empowerment Resource Technologies Ltd. 4. Introduction to Principles in Laboratory Management and Equipment Operations. 4 th -8 th June, Held at BeCa-ILRI, Hub in Nairobi, Kenya. 5. Analysis of Aflatoxin and Fumonisins using ELISA and HPLC; and Molecular characterization of Aspergillus and Fusarium species. Held at University of Nairobi by Safe food Safe Daily project. March 12 th -23 rd Research and Grant Proposal Writing. September Held at University of Nairobi by UNES. 7. Introduction to HPLC course. July By Stuart Jones HPLC Academy. 8. Intermediate HPLC Course. June By Stuart Jones HPLC Academy. 9. Diagnoses of Infectious Diseases by Loop-Mediated Isothermal Amplification (LAMP) th December, ILRI by Asian-Africa Science platform program. 10. Journey into Ministry. October Faith Evangelistic Ministry WORKING EXPERIENCE: POSITIONS HELD Chief Technologist (Laboratory Manager), Department of Public Health Pharmacology & Toxicology 2008 To date Senior Technologist, PHPT Technologist, PHPT RESPONSIBILITIES Chief Technologist; In Public Health, Pharmacology and Toxicology Laboratories of the University of Nairobi. With the capacity to train students at undergraduate and postgraduate levels, conduct research and consultancy work. Curriculum Vitae: Nduhiu Gitahi : 2015 Page 2

3 Specifically involved by offering leadership in the Department in various areas including; Establishment of an ISO accredited Mycotoxins Reference Laboratory Participate in research projects Participate in consultancy work Supervise operations of Immunology, Molecular biology and Microbiology laboratories. Supervise operations of Pharmacology and Toxicology laboratory. Laboratory based training of staff, students and trainees on industrial attachment. Supervise operations of Departmental laboratory animal house. Preparation of Departmental procurement Plan and Budget. Supervision of maintenance of a clean working environment. Supervision of Laboratory Procedures, Sample handling and Analyses for result generation. Development of Laboratory Manuals for staff and students use. Troubleshooting and Maintenance of Laboratory methodologies and equipment. Supervision of Reference stock-cultures and Standard micro-organisms maintenance. Supervision of Departmental Chemical, Reagents and Stationary store keeping. Induction of newly employed staff. RESEARCH PROJECT PARTICIPATED 1. Separation of immunoglobulin in goat milk using electrophoretic and gel chromatographic methods and subsequent conjugation of the polyclonal antibodies for use in ELISA (Enzyme Linked Immunosobent Assay) PhD work for Dr. Solomon S. Munyua. 2. Escherichia coli antibiotic resistant encoding gene, by PCR PhD work for Dr. I. M. Mapenay 3. Genomic DNA isolation and use of bacterial restriction endonuclease DNA analysis in the comparative study of Bacillus cereus emetic toxic and diarrhoeal entorotoxic producing strains PhD work for Prof. J N. Ombui. 4. Diagnosis of Fascioliasis in cattle using ELISA and western blot techniques Msc work for Dr. J. O. Omega. 5. Study of small holders dairy production, -Milk hygiene at formal and informal markets in Nairobi and Nakuru, isolation of Enterohaemorrhagic Escherichia coli, Brucella spp and antibiotic residues Project funded by SDP, Prof. S.M. Arimi. 6. Study of Bruccelosis in cow milk and molecular characterization of Escherichia coli 0157:H7 Curriculum Vitae: Nduhiu Gitahi : 2015 Page 3

4 Msc. work for Dr. E. Koroti. 7. Development of rapid molecular diagnostic techniques for bacterial food poisoning Msc work for Nduhiu Gitahi, IFS funded. 8. Study of bacterial pathogens in fish from government and private ponds Work by Prof. W. O. Ogara. 9. Cloning of Bacillus cereus enterotoxin genes Work by Prof. J. N. Ombui. 10. PCR technology on the study of Bacillus cereus enterotoxin genes Project funded by IFS, Prof. Ombui 11. Characterization of benefits and health risks in urban small holders dairy production, Dagoretti division, Nairobi; - Isolation of Enterohaemorrhagic Escherichia coli, Determination of Cryptosporidium spp. Brucella spp, and Mycobacterium; Assessment of aflatoxin and antimicrobial residues in milk Project funded by IDRC, Prof. E.K. Kengethe 12. The big cats of Samburu, their ecology, conservation and economic impacts of livestock depredation Project fund by Earthwatch, Prof. W.O. Ogara. 13. Urban Disease Emergency (Urban-Zoo) project, University of Liverpool/ University of Edinburgh/ ILRI/KEMRI/APHRC/ University of Nairobi/ IIED/RCV/UCL.- Prof. E.K. Kengethe On-going. 14. Validation of Peepoo for human excreta sanitization and reuse for crop production- Ph.D, Nduhiu Gitahi; Funded by Peepoople Sweden, On-going. SCIENTIFIC REPORTS WRITTEN: Masters of Science (Immunology) Thesis. Nduhiu Gitahi J. (2008). Development of multiplex polymerase chain reaction assay for direct detection of enterotoxigenic Bacillus cereus in food. Diploma in Project management Project Nduhiu Gitahi J. (2011). Factors affecting implementation of school-based reproductive health training programs in Kenya: A survey of schools in Kimathi location, Central Kenya. Higher diploma Medical Laboratory Science project. Nduhiu Gitahi J. (2000). Production of an immunoglobulin-g enzyme conjugate and its use in speciation of meat products. Curriculum Vitae: Nduhiu Gitahi : 2015 Page 4

5 Diploma Medical Laboratory Science Project. Nduhiu Gitahi J. (1994). Isolation, purification, characterization of Goat Immunoglobulin G and production of Rabbit anti Goat conjugate for subsequent use in ELISA. LABORATORY MANUALS & SOPs WRITTEN Isolation and Molecular Identification of Campylobacter species by Multiplex PCR Nduhiu Gitahi and Mainga A.O. Laboratory protocols for isolation and identification of Escherichia coli, Salmonella spp. And Shigella spp from food, water, animal feaces and environment Nduhiu Gitahi and E.K. Kang ethe. Laboratory protocols for sampling water, food, surfaces and animal faeces Nduhiu Gitahi and E.K. Kang ethe Determination of Aflatoxin using Immunochromatographic test. October Nduhiu Gitahi and E.K. Kang ethe. Practical Immunology Manual, 1 st Edition. (2001) E. K. Kangethe and Nduhiu Gitahi J. Determination of Aflatoxins M1 in Milk using enzyme Linked Immunosorbent Assay. May Nduhiu Gitahi and E.K. Kange the. Using Rida Soft win (ART.NO.Z9999) ELISA results generation. May Nduhiu Gitahi and E.K. Kange the. Determination of aflatoxins total in feeds using Enzyme Linked Immunosorbent Assay. May Nduhiu Gitahi and E.K. Kang ethe. Antibiotics Residue Analysis HPLC, (2008) Nduhiu Gitahi J. and J. G. Nderitu J. Quatitative analysis of aflatoxin, Enzyme Immunoassay, (2008) Nduhiu Gitahi J. Meat Speciation, Ochterony Double Diffusion Method, (2008) Nduhiu Gitahi J. Preparation of Genomic DNA from Bacteria, (2008) J. N. Ombui and Nduhiu Gitahi J. Laboratory manual for best laboratory practices for training of traineers Somalia Nduhiu G. CONSULTANCY REPORTS Mycotoxins training in six (6) Counties and at the National level; FAOR/LoA No 018/2015. (2015) Kang ethe E.K, Muthomi J.W., Nduhiu Gitahi and Nyaga P.N.; FAO Ongoing. Sanitization of human feases in Peepoos used in field setup. (2014). Gathura P.B., and Nduhiu Gitahi. Peepoople AB, SE Stockholm Sweden. Curriculum Vitae: Nduhiu Gitahi : 2015 Page 5

6 Sanitization of human feases when simulating field use of Peepoo. (2013). Ombui J.N., and Nduhiu Gitahi. Peepoople AB, SE Stockholm Sweden. Assessment of effectiveness of Peepoo bags on sanitization of human faeces. (2013). Ombui J.N., and Nduhiu Gitahi. Peepoople AB, SE Stockholm Sweden. Analysis of Camel milk for heavy metals, drugs, pesticide residues and aflatoxin M1. (2012). Ministry of Livestock development. Mbaria J.M., and Nduhiu Gitahi. Assessment of effectiveness of Peepoo bags on sanitization of human faeces. (2011). Senerwa D.M., and Nduhiu Gitahi. Peepoople AB, SE Stockholm Sweden. Analysis of meat for heavy metals and organochlorine pesticids residuals and milk for aflatoxin M1. (2011). Ministry of Livestock development. Kanja N.L, Kitala P.M., Kahunyu J.M., and Nduhiu Gitahi. Development of a workable disease control strategy in cattle. (2010). Maitho T., Nduhiu Gitahi J. and Macharia S.M. Ministry of Livestock development, Smallholder dairy commercialization programme. Assessment of food and dairy oriented analytical laboratories in Kenya (2009). Jackson N. Ombui, Joseph M. Gathuma, Daniel M. Senerwa, Nduhiu Gitahi J, Hezron Wimbia, and Humphrey Kaburia. For Lands O Lakes, USAID Project. PUBLICATION PUBLICATION IN PEER REVIEWED JOURNALS: 1. Njue L. G., Ombui J.N., Kanja L.W., Gathumbi J.K, and Nduhiu J.G Evaluation of oral toxicity level of ethyl acetate extract, from garlic (allium sativum) in onorrh dawleys rats as per OECD guidelines 423. J. Food Sci. Technol, 2(3): pp Njue L. G., Kanja L.W., Ombui J.N. Nduhiu J.G. and Obiero, D Efficacy of antimicrobial activity of garlic extracts on bacterial pathogens commonly found to contaminate meat. E. Arf. Med. J. Vol.9 No Kabui K.K., Arimi S.M., Kang ethe E.K., Omore A., Makokha S., Nduhiu G., Mainga A.O. and Macharia J.K A determination of raw milk quality and the most suitable microbiological test at the milk collection level in two regions of Kenya. 4. Gathumbi J.K., Kanja L.W., Maitho T.E., Mbaria J.M., Nduhiu J.G., Gitau F.K., Nderitu J.G., Lucy M.W, and Maloba K Assessment of lead and copper in fish and soil sediments in Kirinyaga South District, Kenya. Journal of Applied Sciences in Environmental Sanitation, 8 (3): Curriculum Vitae: Nduhiu Gitahi : 2015 Page 6

7 5. William O. Ogara, Nduhiu J. Gitahi, Samuel A. Andanje, Nicolas Oguge, Dorcas W. Nduati and Alfred O. Mainga Determination of carnivores prey base by scat analysis in Samburu community group ranches in Kenya. African Journal of Environmental Science and Technology Vol. 4(8), pp , August Available online at X 2010 Academic Journals. 6. Onono J.O., Ogara W.O., Okuthe S.O., Nduhiu J.G., Mainga A.O. and Nduati D Challenges of camel production in Samburu District, Kenya. Journal of Camel Science 3, Cyrus G. Wagate, James M. Mbaria, Daniel W. Gakuya, Mark O. Nanyingi, P. G. Kareru, Anne Njuguna, Nduhiu Gitahi, James K. Macharia and Francis K. Njonge. Screening of some Kenyan Medicinal Plants for Antibacterial Activity. Phytother. Res., DOI: /ptr Copyright 2009 John Wiley & Sons, Ltd. 8. Nduhiu Gitahi J., Jackson N. Ombui, Dorcas W. Nduati, Michael M Gicheru. Genetic Characterisation of food borne Bacillus cereus strains from milk, cheese and rice by multiplex PCR assay. Inter J. of Integrative Biology, 2009, 5, (2) Mbaka M., Gathura P. B., Njeru F.M., Gitahi J.N., et al., An assessment of the presence of Escherichia coli in the roof-collected rainwater from some areas around Nairobi. The Kenya Veterinarian, 23, Ombui J. N., J. Nduhiu Gitahi., Gicheru M. M., Direct detection of enterotoxigenic Bacillus cereus in processed food by multiplex Polymerase Chain Reaction. Inter J. of Integrative Biology, 2008, 2, (3) Ombui, J.N., and Nduhiu J.G., Macharia J.K. Immunoassay and polymerase chain reaction techniques for detection of enterotoxigenic Bacillus cereus.. E. Afr. Med. J. 2005, 82, No. 8, Ombui, J.N. and Nduhiu J.G. Prevalence of enterotoxigenic Bacillus Cereus and its enteroxins in milk and milk products in and around Nairobi. E. Afr. Med. J. 2005, 82, No. 6, Ombui J.N., A.M. Kimotho and J.G. Nduhiu. Antimicrobial resistance patterns and plasmid profiles of staphylococcus aureus isolated from milk and meat. E. Afr. Med. J. 2000, 77, Ombui J.N., Mathenge J.M., Kimotho A.M., Macharia J.K. and Nduhiu J.G. Frequency of antimicrobial resistance and plasmid profiles in Bacillus cereus strains isolated from milk. E. Afr. Med. J. 1996, 73, Ombui, J.N., Macharia J.K. and Nduhiu J.G. Frequency of antimicrobial resistance and Curriculum Vitae: Nduhiu Gitahi : 2015 Page 7

8 plasmid profiles of Escherichia coli isolated from milk. E. Afr. Med. J. 1995, 72; Ombui J.N., Kaburia H.F.A., Macharia J.K. and Nduhiu J.G. Coliform counts and Escherichia coli in raw milk from dairy farmers in Kiambu district, Kenya E.9 Afr. Med. J. 1994, 71; CONFERENCES PAPERS: 1. Nduhiu Gitahi, Ogeto Mwebi, Tom Gnoske, Troy Nowark and Julian Kebis Peterhans. Forensic insights into the predatory behavior of the Man-Eaters of Tsavo Presented at 5 th EAAPP conference, held at Dar es Salaam. 3 rd -6 th August. 2. Akale J.E., Maina C.W., Nduhiu G., Mainga A., Macharia J.K., and Kang ethe E.K. Prevalence of cryptosporidia in dogs and cats from selected kernels and veterinary clinics in Nairobi, Kenya Presented at 9 th Biennial scientific conference and Exhibition, held at University of Nairobi Kenya 3 rd 5 th September Mbaria J.M., Ombui J.N., Nduhiu J., Nguta J.M., Kahunyo J.M. and Nderitu J.G. Drug and chemical residues in camel milk in Nanyuki, Kenya Presented at 9 th Biennial scientific conference and Exhibition, held at University of Nairobi Kenya 3 rd 5 th September William O. Ogara 1, Nduhiu Gitahi 1, Samuel A. Andanje 2, Nicolas Oguge 3, Alfred O. Mainga 1, Dorcas W. Nduati 1. One health approach to an integrated ecosystem study; A case of human carnivore conflict in Samburu County, Kenya EAQUA. Presented at Sportmans Arms, Nanyuki, Kenya. 5. Nduhiu Gitahi J. Laboratory aspects of HIV/AIDS diagnosis and monitoring antiretroviral therapy. Seminar presented at University of Nairobi, Department of Public health Pharmacology & Toxicology 9 th April Wagate G. C., Gakuya D. W., Nanyingi M. O., Njoroge F. K., Nduhiu G. and Mbaria J. M. Antibacterial and cytotoxic activity of some Kenyan medicinal plants. Read at 6 th Biennial scientific conference and Exhibition, held at University of Nairobi Kenya 17 th 19 th September Ogara W. O., Nduhiu Gitahi J., Andanje S. A. and Oguge N. O. Determination of carnivore prey base in Samburu community group ranches by scat analysis. Read at 6 th Biennial scientific conference and Exhibition, held at University of Nairobi Kenya 17 th 19 th September Nduhiu Gitahi J., Ombui J. N. and Nduati D.W. Characterisation of food-borne enterotoxigenic Bacillus cereus strains from milk, cheese and rice by multiplex PCR assay. Read at 6 th Biennial scientific conference and Exhibition, held at University of Nairobi Curriculum Vitae: Nduhiu Gitahi : 2015 Page 8

9 Kenya 17 th 19 th September S. M. Arimi, E. Koroti, E. K. Kang ethe, A. O. Omore, J. J. McDermott, J.G. Nduhiu, J. K. Macharia and A. M. Githua. Risk of infection from E.coli 0157:H7 through informally marketed raw milk in Kenya. Read at 3 rd African conference on Animal Agriculture and 11 th conference of the Egypt Society of Animal product held at Alexandria Egypt 6 th 9 th November Aboge, G. O., Kang ethe E. K., Arimi, S. M., Kanja L.W., Omore A.O, McDermott J. J. J.G. Nduhiu, J.K. Macharia, AM. Githua, Antimicrobial agents detected in marketed milk in Kenya. Read at 3 rd All African Conference on Animal Agriculture and 11 th conference of the Egypt Society of Animal product held at Alexandria Egypt 6 th 9 th November Kang ethe, E. K, Arimi, S. M., Omore A. O., McDermott J. J., Nduhiu J.G. The prevalence of antibodies to Brucella abortus in marketed milk in Kenya and its public health implications. Read at 3 rd All African conference on Animal Agriculture and 11 th conference of the Egypt Society of Animal product held at Alexandria Egypt 6 th 9 th November STUDENT SUPERVISION 1. Bachelor of Science in Medical Microbiology, Naftaly Mureithi Final year project Phenotypic and genotypic characterization of antimicrobial resistance in hospital isolated Salmonella typhi. Jomo Kenyatta University of Agriculture and technology. WORKSHOPS AND CONFERENCES ATTENDED: Eppendorf Clinic. EME-0612-KEN-050. Laboratory Management and Equipment Operations Workshop. By Eppendorf Middle East with F&S Scientific Ltd. At ILRI, Kenya. 8 th June, Advances in pest management and the role of veterinarians. University of Nairobi, Department of Public Health Pharmacology & Toxicology. 16 th September, Alternatives to use of Animals in Experiments in Education and Training, Nairobi, Kenya. By African network for animal welfare and The international network for human education th September, Roche Applied Science High Throughput Sequencing Workshop held at BecA/ILRI, Nairobi, Kenya. 12 th February, th Biennial Scientific Conference & Exhibition, th -19 th September, University of Nairobi, CAVS. Roche Applied Science Inspiring Discovery Workshop for Molecular & Cellular Sciences, Nairobi, Kenya, 2 nd October Curriculum Vitae: Nduhiu Gitahi : 2015 Page 9

10 REGISTRATION: The Kenya Medical Laboratory Technicians and Technologists Board. REG. NO. A0622 OTHER RESPONSIBILITIES: Field Team Leader Carnivore Project, Samburu Conservation Research Initiative, Chairman- Upper Kabete Staff Christian Fellowship, REFEREES: Professor E.K. Kang ethe University of Nairobi Department of Public Health Pharmacology & Toxicology P. O. Box NAIROBI. Tel Dr. Michael M. Gicheru Kenyatta University Department of Zoological Sciences, P.O. Box NAIROBI Tel Professor W. O. Ogara University of Nairobi Department of Public Health Pharmacology & Toxicology P. O. Box NAIROBI. Tel Curriculum Vitae: Nduhiu Gitahi : 2015 Page 10

C U R R I C U L U M V I T A E. NAME : Prof. Samuel Mutwiri Arimi. PERMANENT ADDRESS : P.O. Box 73, Nkubu, Meru, Kenya

C U R R I C U L U M V I T A E. NAME : Prof. Samuel Mutwiri Arimi. PERMANENT ADDRESS : P.O. Box 73, Nkubu, Meru, Kenya C U R R I C U L U M V I T A E NAME : Prof. Samuel Mutwiri Arimi PERMANENT ADDRESS : P.O. Box 73, Nkubu, Meru, Kenya PRESENT ADDRESS : Department of Public Health, Pharmacology & Toxicology, College of