Background 1 st, 2 nd and 3 rd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with an Emphasis on Brucella melitensis (in

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2 Background 1 st, 2 nd and 3 rd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with an Emphasis on Brucella melitensis (in collaboration with DLD) Brucellosis OIE Twinning Laboratory Programme France-Thailand The 4th FAO-APHCA/OIE/DLD Regional Workshop on Brucellosis Diagnosis and Control in Asia-Pacific Region -Proficiency Test and Ways Forward- Chiang Mai, March 2014 The 1 st and 2 nd Asia-Pacific Bovine Brucellosis Proficiency Test (2013, 2014) Outcome and ways forward

3 The Minutes of 65 th Executive Committee Meeting and the 29 th Session of APHCA (25 September 2005) Thailand - Raised the re-appearance of Brucella melitensis and its threat - Requested APHCA to organise a relevant workshop. Supported by delegates.

4 Report of the 30th APHCA Session (Luang Prabang, Lao PDR) Wednesday, 25 October Review of day 1 country priorities for action(s) APHCA-OIE Regional workshop on goats (chaired by C. Leowijuk) i) Brucella melitensis in goats diagnosis, vaccination and other trade related technical protocols by B. Garin-Bastuji, c/o OIE; ii) Special presentation on OIE pathway for BSE-free status declaration by Y. Oketani, OIE-Tokyo Office; and iii) General discussion on disease issues in goats

5 Minutes of the 31st Session of APHCA October 29 th -November 1 st 2007, Chiang Mai, Thailand Future workplan/activities: 2008 period Continued activities from 2007 New Initiatives: 1/4 topics. Proposed Diagnosis and control of Brucella melitensis and Bluetongue

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7 1 st FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis Chiang Mai and Lampang, October member countries Lectures and lab practiced on serological tests National labs: keep same SOP according to OIE standard

8 1 st FAO-APHCA/OIE Regional Workshop Member countries: requested - Follow up workshop/training - Continue capacity building of national veterinary services

9 2 nd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis Khon Kaen, 8-11 June member countries Lecture and lab practiced SOP (based on OIE Manual) RBT antigens were provided

10 2 nd FAO-APHCA/OIE Regional Workshop Requested: - Quality assurance including standardisation - Strengthening of Regional collaboration on diagnosis and control of brucellosis Recommendation for OIE Twinning Programme

11 3 rd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis Sukhothai and Phitsanulok, November member countries Lecture and lab practiced serological tests and reagents control Demonstration: Brucella spp. isolation, identification and typing (BSL-3)

12 3 rd FAO-APHCA/OIE Regional Workshop Observation and suggestion: The member countries - utilized the knowledge and techniques - improved/strengthened their laboratory diagnosis capacity Under the OIE Twinning Programme - the participants requested for support on Brucellosis proficiency testing to member countries in the region

13 OIE Reference Laboratory Twinning Programme: Brucellosis, France-Thailand Birth of collaboration - 1 st FAO-APHCA/OIE Regional Workshop - 2 nd FAO-APHCA/OIE Regional Workshop The Brucellosis OIE Twinning Program France-Thailand (ANSES & NIAH-DLD) - OIE expert: Dr. Bruno Garin-Bastuji, ANSES - Three-year project:

14 OIE Reference Laboratory Twinning Programme: Brucellosis

15 OIE Reference Laboratory Twinning Programme: Brucellosis Brucellosis twinning programme was completed on December 31, 2013 but collaboration still goes on..

16 OIE Reference Laboratory Twinning Programme: Brucellosis Thailand-Japan Joint Conference on Animal Health 2012 Prince Mahidol Award Conference, Jan 2013, Bangkok

17 OIE Reference Laboratory Twinning Programme: Brucellosis NIAH and RVRDCs poster presentations: brucellosis laboratory surveillance and Twinning for 6 posters (Thailand-Japan Joint Conference on Animal Health 2012)

18 Preparation: The 1 st Asia-Pacific Bovine Brucellosis Proficiency Test 2013 Under OIE Twinning programme & FAO-APHCA and supervision by Dr. Bruno Garin-Bastuji Panels of sera preparation: July 2013 Distribution Report the results in 2014

19 The 4th FAO-APHCA/OIE/DLD Regional Workshop on Brucellosis Diagnosis and Control in Asia-Pacific Region -Proficiency Test and Ways Forward- Chiang Mai, March 2014 Review and update on brucellosis Quality management in veterinary testing lab International shipment PT 2013 results Distributed PT 2014

20 Workshop proposed: Share information for strengthening the regional networking FAO-APHCA and OIE RRAP should continue providing collaborative support to strengthen Brucellosis diagnostic capacity building OIE/FAO Ref Lab should continue to provide technical support Possible.. Refresh/update hands-on WS on Brucellosis Diagnosis (upon request)

21 Results: The 1 st Asia-Pacific Bovine Brucellosis Proficiency Test 2013 Participants 34 labs (results 33 Labs) Asia-pacific countries 18 countries, results returned 17 labs Regional VRDCs-NIAH 8 labs Government enterprise 1 lab Universities 4 labs Private companies 2 labs ANSES

22 The 1st and 2nd Asia-Pacific Bovine Brucellosis Proficiency Test (2013, 2014)

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24 Outcome and ways forward

25 Laboratory Quality assurance: NIAH & RVRDCs Animal Health Laboratories - NIAH, Bangkok - OIE Regional Reference Laboratory for FMD - Veterinary Biological Assay Center /ASEAN Assay Lab - 8 Regional Veterinary Research and Development Centers (7+1) Songkhla

26 Laboratory Quality assurance: NIAH & RVRDCs - Serological test for Brucellosis was accredited: RBT and CFT (NIAH and 7 RVRDCs) ISO 9001:2008

27 Brucellosis Research collaboration QIA-Korea and NIAH-DLD-Thailand: The molecular epidemiological analysis of Brucella species from a variety of animals and application on diagnostics for Brucella canis in Thailand NIAH-Japan and NIAH-DLD-Thailand: June 2014-March 2017 Development of Milk-ELISA kit for detection of antibody against Brucella sp.

28 @ Annual Meeting of Brucellosis Further study: 1 MSc and 1 PhD (Brucellosis Lab)

29 PVS Evaluation, 2012 PVS Gap Analysis, 2014

30 Discussion and needed for brucellosis diagnosis and control th FAO-APHCA/OIE/DLD Regional Workshop Bovine PT (2013 and 2014) FAO-APHCA/OIE Regional Workshops (1 st, 2 nd and 3 rd WS) 2008 Present NIAH-DLD brucellosis related activities and lesson learned, ISO/IEC 17025:2005 and ISO 9001:2008, research collaboration FETPV, One Health OIE-PVS OIE Lab Twinning Programme on Brucellosis (France- Thailand) Application as OIE Ref Lab for Brucellosis

31 Understanding Communication, Team and trust Coordination & Collaboration Control brucellosis in the region Brucellosis laboratory network in Asia-Pacific region Disease Control Regional Network and need support from international organisations

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35 Regional Veterinary Research and Development Centers (8 Labs) Bureau of Disease Control and Veterinary Services, DLD. Bureau of Epidemiology, Department of Disease Control, MOPH National Institute of Animal Health

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Brucellosis OIE Twinning Laboratory Program France-Thailand

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