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4 " F K$LD #J (1385 I@)?% (#G6 H) D EF< =>% 9?@ A?B C Downloaded from journal.bums.ac.ir at 1:43 IRDT on Friday March nd '6 1 RH( Z. $ " ;6 +* '6 1 RH( Z. Ah;,-. +* '6 1,-. Z. h;,-. 1,-. M> ;6 _(10) (H Ñ%. 6x< [B ()w _h* MC '! N tr9 '( ^6 6 * 1,-. 4E D:.(13A10) ($ ;6 ^] (. " 9$ '($?@ =- M9: A'6 1,-. '($ (< '($?@ M9:.(13A11A10) ($ +( 1,-. ;6 K.(1 A8-5) ($ +(?%. <? 6 A78 9: '($ (< +)< 6 N=9. ()w _(] )$ 4G.( M]k PCR L 3 4 (%0/6) => 1,-. ;6 R$ <. 'B '6 1,-. <. "]! "?r A; H ^] (..h* MC 'o <. 6 Ö] (3K "$(3 '/ / 'K o* "( "8 mo* ){ _" '(?@ ()< $*?=&.,E -H!8 G6 M8 (G. mo*?@ "3<.; 01 /0* hd 'H 1,-. ; A" hd '.($ x<! ] ($ '6 " m(*.(9) " M% =- N) ;6 /0?p. +( $ "%& '()* & "$(3 4 5 )& 6 ' 1,-..() (' 1,-. %0/6 A'($?@ NB>. '($?@ M9:.( '6 A (' L; %13/3. %C ;6 * '( Çw () $; ){ _(10) M$; ^=d.(11) " '($? & %6 A" R$ 8 A 1,-. ;6 $; A)} 3)..(8-5) ($ ;6 %1 " '? & %1/66 ($ / E (' (9.(1) =< %=- y$ $ (. A;6.(14A13) ($ B:) " A'6 1,-.? 5 3). 78 9:?@ =- M9:. '6 6 M>.(15) (' É.% "]! A '($ * d, A Little 9: ;6 (%6/36) 36 (& 10 M> 3). A($ '6 Suresh 9: 8 _(10) "$ ;6 A()$ '6 *? 5 1,-. *.(13) ( E M>.; '6 " =- MC :51$ 1- Trepka MJ, Archer JR, Altekruse SF, Proctor ME, Davis JP. An increase in sporadic and outbreak-associated Salmonella enteritidis infections in Wisconsin: the role of eggs. J Infect Dis. 1999; 180 (4): Binkin N, Scuderi G, Novaco F, Giovanardi GL, Paganelli G, Ferrari G, et al. Egg-related salmonella enteritidis, Italy, Epidemiol Infec. 1993; 110 ():

5 1388 "#$% 16 Downloaded from journal.bums.ac.ir at 1:43 IRDT on Friday March nd Morse DL, Birkhead GS, Guardino J, Kondracki SF, Guzewich JJ. Outbreak and sporadic egg-associated cases of Salmonella enteritidis: New York's experience. Am J Public Health. 1994; 84 (5): Braden CR. Salmonella enterica serotype Enteritidis and eggs: a national epidemic in the United States. Clin Infect Dis. 006; 43 (4): Jamshidi AA. Determination of the incidence of Salmonella contamination of eggs being consumed in Shiraz. Journal of Babol University of Medical Sciences. 1999; 5 (): 5-1. [Persian] 6- Oboodi B, Farboud A, Masoudi M, Pourabbas B, Alborzi A, Karimi A. Salmonella contamination of industrial and non-industrial produced eggs in Shiraz. Iranian Journal of Infectious Diseases & Tropical Medicine. 001; 14(6): [Persian] 7- Hadian Z, Valaii N, Himi P. Prevalence of Salmonella contamination in consumed egg yolks in Tehran during (1997). Pejouhandeh Quarterly Research Journal. 1999; 14 (4): [Persian] 8- Hamedi AK, Parizadeh MJ, Ghenat J. Study of contamination of Salmonella in two groups egg (Commercial and local). Medical Journal of Mashhad University of Medical Sciences. 00; 76 (45): [Persian] 9- Editorial team. Large variation in prevalence of salmonella in laying hen flocks in EU: EFSA preliminary report. Euro Surveill. 006; 11(6): E Available From: Little C, Walsh S, Hucklesby L, Surman-Lee S, Pathak K, Hall Y, et al. Salmonella contamination in non-uk produced shell eggs on retail sale in some regions of England. Euro Surveill. 006; 11(11): E Available From: Little CL, Surman-Lee S, Greenwood M, Bolton FJ, Elson R, Mitchell RT, et al. Public health investigations of Salmonella Enteritidis in catering raw shell eggs, Lett Appl Microbiol. 007; 44(6): Jafari RA, Fazlara A, Dalirannia A. An Investigation Into Salmonella Contamination Of Native Hens' Eggs In Ahvaz. Scientific-Research Iranian Veterinary Journal. 006; (13): [Persian] 13- Suresh T, Hatha AA, Sreenivasan D, Sangeetha N, Lashmanaperumalsamy P. Prevalence and antimicrobial resistance of Salmonella enteritidis and other salmonellas in the eggs and egg-storing trays from retail markets of Coimbatore, South India. Food Microbiol. 006; 3 (3): Mollenhorst H, van Woudenbergh CJ, Bokkers EG, de Boer IJ. Risk factors for Salmonella enteritidis infections in laying hens. Poult Sci. 005; 84 (8): Reu K DE, Messens W, Heyndrickx M, Rodenburg TB, Uyttendaele M, Herman L. Bacterial contamination of table eggs and the influence of housing systems. World's Poultry Science Journal. 008; 64:

6 " F K$LD #J (1385 I@)?% (#G6 H) D EF< =>% 9?@ A?B C Abstract Original Article Prevalence of salmonella contamination in locally Downloaded from journal.bums.ac.ir at 1:43 IRDT on Friday March nd 019 (non-industrially) produced eggs in Birjand (006) M.H. Namaei 1, M. Ziaee, M. Ghannad Kafi 3 Background and Aim: Salmonella is one of the common bacterial pathogens between man and birds throughout the world. Inconsistent Salmonella contamination following consumption of eggs, as one of the main contributors of the disease has also become an important public health issue. The aim of this study was to investigate prevalence of Salmonella contamination in locally-produced eggs in Birjand in 006. Materials and Methods: In this cross-sectional study, raw non industrialized eggs in Birjand were evaluated for Salmonella contamination. A total of 500 samples were purchased from retail outlets throughout the city. Both the shells and contents of the eggs were cultured using the standard method. Biochemical tests, Salmonella serotyping and PCR were applied to specify and confirm of Salmonella isolates. Results: The overall finding was that 3 samples (0.6%) were contaminated with Salmonella. In two out of 3 contaminated eggs, salmonella was found on shell only; both the shell and content of the other one were contaminated. Conclusion: Prevalence of Salmonella contamination among the barn and free range eggs in Birjand was 0.6%, which is equivalent to approximately 1 in every 165 eggs. This is in accordance with other surveys in Iran (Shiraz, Tehran and Mashhad). Based on the results, to prevent salmonellosis, proper cooking of the eggs is recommended. Key Words: Salmonella; Salmonellosis; Gastroenteritis; Egg Journal of Birjand University of Medical Sciences. 009; 16 (): Received: Last Revised: Accepted: Corresponding Author; Assistant Professor, Department of Microbiology, Faculty of Medicine, Birjand University of Medical Sciences. Birjand, Iran mhnamaei@hotmail.com Associate Professor, Department of Social Medicine, Faculty of Medicine, Birjand University of Medical Science, Birjand, Iran 3 MS, Birjand University of Medical Science