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4 9 / ) (.().()... Muma JB, Samui KL, Oloya J, Munyeme M, Skjerve E. Risk factors for brucellosis in indigenous cattle reared in livestockwildlife interface areas of Zambia, 007, Preventive Veterinary Medicine 80: Omer MK, Skjerve E, Woldehiwet Z, Holstad G. Risk factors for Brucella spp. Infection in dairy cattle farms in Asmara state of Eritrea. 000, Preventive Veterinary Medicine 6: Stringer LA, Guitian FJ, Abernethy DA, Honhold NH, Menzies FD. Risk associated with animals moved from herds infected with Brucellosis in Northern Ireland. 008, Preventive Veterinary Medicine 8: Iranian Veterinary Organization. National program for Brucellosis control; 0. Zeinali M, Shirzadi MR. National guideline for Brucellosis control, Ministery of Health and Medical Education, 007 ; Amiri K. Economical loss estimation due to brucellosis control program stop in animal population in Iran, 007, nd National Iranian Congress of Brucellosis, 9- May 007, Tehran- Iran, Rasouli J, HolakoueiNaeini K, Forouzanfar MH, SalarilakSh, Bahonar M, Rashidian A. Cost effectiveness of livestock vaccination for Brucellosis in West Azerbayjan province,..(8).( ) ()...(9 0) 009, The Journal of Urmia University of Medical sciences, 0: Bahonar AR, HolakoueiNaeini K, Nadim A, Zahedi MR, Zowghi E, Mohammad K. Brucellosis determinants in Chaharmahal and Bakhtiari province, Iran. 00, Payesh; : Coelho AM, Coelho AC, Roboredo M, Rodrigues J. A casecontrol study of risk factors for Brucellosis seropositivity in Portuguese small ruminants herds, 007, Preventive Veterinary Medicine 8: Matope G, Bhebhe E, Muma JB, Lund A, Skjerve E. Herdlevel factors for Brucellaseropositivity in cattle reared in smallholder dairy farms of Zimbabwe, 00, Preventive Veterinary Medicine 9: -.. Zowghi E, Ebadi A. Naturally occurring Brucellamelitensis infection in cattle in Iran. Rev. sci. tech.off. int. Epiz., 98; : 8-. Nilson K, Duncan JR. Animal Brucellosis, Zowghi, E (translator in Persian), Ghalamestanehonar press,

5 /0 Iranian Journal of Epidemiology 0; 8(): 6-0. Original Article Outbreak Investigation of Brucellosis in an Industrial Dairy Cattle Farm in Isfahan Province-Iran Bahonar AR,, Amiri K, Akbarein HD, Rasoli Beirami N, Amiri HR, Imani Tabar F, Khajeh Nasiri SH, Arab Zadeh S, Moghaddas V, Makenali AS - Faculty of veterinary medicine, Tehran university, Iran - Research center of zoonoses, Tehran University of medical sciences, Tehran, Iran - Iranian veterinary organization - - Pasteur veterinary laboratory Corresponding author: Bahonar A., abahonar@ut.ac.ir Background & Objectives: Prevention and control of brucellosis in animals is the main route of its prevention in human. After detecting a brucellosis outbreak in an industrial dairy cattle farm in Isfahan province in Iran, an epidemiological investigation was carried out to determine prevalence of seropositive cows and incidence rate inorder to eliminate positive couws. Methods: This study was carried out to determine abortion rates in caws. RBPT, SAT and ME serologic tests were used for detecting infected cows and Brucella agar for isolation of bacteria. Data analysis was performed by Chi-square test and calculation of cumulative incidence ratio. Results: Of total 9 female cows above months age, 706 infected animals were found during investigation (March-December 0). Incidence of abortion in infected cows was significantly higher than no infected (% versus %, P<0.0). Culture for 7 milk samples was positive for brucella out of 00 milk samples. Conclusion: Attention to using reduce dose of RB vaccine, controlling of import animal in farm besides biosecurity are the main factors for prevention of similar outbreaks in dairy farms. Keywords: Brucellosis, Dairy cattle, Iran