Levels of MRSA on pigs and environmental samples

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1 Downloaded from orbit.dtu.dk on: Dec 18, 2017 Level of MRSA on pig and environmental ample Hanen, Julie Elvekjær Publihed in: Book of preentation of the 3rd CPH Pig eminar Publication date: 2016 Document Verion Publiher' PDF, alo known a Verion of record Link back to DTU Orbit Citation (APA): Hanen, J. E. (2016). Level of MRSA on pig and environmental ample. In Book of preentation of the 3rd CPH Pig eminar: Up to date with pig reearch (pp ). Copenhagen Univerity. General right Copyright and moral right for the publication made acceible in the public portal are retained by the author and/or other copyright owner and it i a condition of acceing publication that uer recognie and abide by the legal requirement aociated with thee right. Uer may download and print one copy of any publication from the public portal for the purpoe of private tudy or reearch. You may not further ditribute the material or ue it for any profit-making activity or commercial gain You may freely ditribute the URL identifying the publication in the public portal If you believe that thi document breache copyright pleae contact u providing detail, and we will remove acce to the work immediately and invetigate your claim.

2 T H E F A C U L T Y O F H E A L T H A N D M E D I C A L S C I E N C ES U N I V E R S I T Y O F C O P E N H A G E N C E N T E R F O R R E S E A R C H I N P I G P R O D U C T I O N A N D H E A L T H Book of preentation of the 3 rd CPH Pig eminar February 3, 2016 Up to Date with Pig Reearch

3 Table of Content Programme... iv Lit of participant... vi Key Lecture: Benefit of linking univeritie and the Danih pig indutry from the indutry point of view... 1 Seion 1: Growing Pig... 7 PCV2 infection dynamic: Diagnotic, between-batch conitency and correlation to productivity parameter... 8 A multivariate dynamic linear model for early warning of diarrhea and pen fouling in laughter pig. 13 Oteomyeliti in Danih laugther pig Monitoring growth in finiher by weighing elected group of pig Seion 2: Sow and Piglet Monitoring PRRS uing laboratory data Impact of feeding regime on growth in prepubertal gilt Increaing the dietary level of protein for lactating ow affect litter gain and ow weight lo Gatric emptying rate and blood value in newborn intra-uterine growth retricted piglet Seion 3: Welfare The ue of virtual herd in veterinary and agricultural education Aeing farm with the Danih animal welfare index Tail biting: prevalence among docked and undocked pig from weaning to laughter Looe houed ow with low piglet mortality Seion 4: MRSA Level of MRSA on pig and environmental ample Can we reduce MRSA ST398 in poitive farm by eliminating a few pig carrier? How to pool fecal ample in a cro-ectional tudy of antimicrobial reitance gene in Danih pig herd Can pooled faecal ample be ued for reitance profiling? -Reitance in E. coli iolate from diarrhoeic nurery pig

4 ii

5 Preface The Center for reearch in pig production and health CPH Pig enhance, conolidate and raie the profile of pig production reearch. The importance of reearch and innovation for the Danih pork indutry remain crucial. The Danih pig producer face tight financial preure and many factor contribute to the volatility urrounding the pork indutry. The production of finiher in Denmark i declining a producer export a ignificant and increaing number of weaner to be finihed elewhere in Europe. A goal of CPH Pig i, in collaboration with it many partner, to upport the Danih pig indutry with R&D that i eential in addreing driver of a profitable and utainable pork production in a global market. The 2016 eminar preent the recent development acro a divere range of dicipline impacting on pig production particularly on increaed productivity, urvival among piglet, MRSA, reduction in antibiotic uage and objective meaurement of animal welfare. With thi in mind, the reearch preentation of the CPH Pig eminar will cover four main theme: Growing pig, Sow and Piglet, Welfare and MRSA. It i our hope that thi third CPH Pig eminar will provide an excellent forum to preent new finding, foter in-depth dicuion, and hopefully provide olution to ome of the indutry challenge. The Univerity of Copenhagen and CPH Pig are integral in the training of undergraduate and potgraduate tudent and generating junior cientit who are eential to the future of the indutry. CPH Pig outwardly promote the involvement of tudent and early-career cientit at the meeting. The eminar again ha a coniderable number of young cientit attending and preenting their work and for many it will be their firt opportunity to interact with key indutry figure and organiation. It i a pleaure to direct the CPH Pig eminar and contribute to facilitating the networking of thoe involved in pig reearch and pork production. Thi eminar i poible becaue a dedicated group of individual coordinated and arranged all apect of the event. We wih to thank everyone for their contribution and dedication. However, mot importantly, thank you for participating and making the eminar a great ucce. Thank you for your upport and we hope you will continue to be part of the future achievement of the center. Han Henrik Dietz Chairman Chritian Fink Hanen Center Director iii

6 Programme CPH Pig Seminar February 3, 2016 Up to Date with Pig Reearch Time Preenter Title 9:00-9:10 9:10-9:30 Han Henrik Dietz Seion 1: Growing pig 9:30-9:45 9:45-10:00 10:00-10:15 10:15-10:30 Head of Department, Department of Large Animal Science, Univerity of Copenhagen Jen Ulrich Nielen Director, Innovation, SEGES Pig Reearch Centre Gitte Blach Nielen, Indutrial PhD tudent, Department of Large Animal Science, Univerity of Copenhagen & MSD Animal Health Dan Børge Jenen, PhD tudent, Department of Large Animal Science, Univerity of Copenhagen Louie Krue Jenen Aitant Profeor, Department of Veterinary Dieae Biology, Univerity of Copenhagen Anna Helena Stygar, Pot doc., Department of Large Animal Science, Univerity of Copenhagen 10:30-11:00 MORNING TEA/COFFEE Seion 2: Sow and Piglet 11:00-11:15 11:15-11:30 11:30-11:45 11:45-12:00 12:00-13:00 LUNCH Ana Carolina Lope Antune PhD Student, DTU National Veterinary Intitute Sophie Van Vliet PhD tudent, Aarhu Univerity Thoma Sønderby Bruun, Senior Specialit, SEGES Pig Reearch Centre Charlotte Amdi William, Pot doc., Department of Large Animal Science, Univerity of Copenhagen Welcome Key Lecture: Benefit of linking univeritie and the Danih pig indutry from the indutry point of view Chair: Jen Peter Nielen PCV2 infection dynamic: Diagnotic, between-batch conitency and correlation to productivity parameter A multivariate dynamic linear model for early warning of diarrhea and pen fouling in laughter pig Oteomyeliti in Danih laughter pig Monitoring growth in finiher by weighing elected group of pig Chair: Anne-Helene Tauon Monitoring PRRS uing laboratory data Impact of feeding regime on growth in prepubertal gilt Increaing the dietary level of protein for lactating ow affect litter gain and ow weight lo Gatric emptying rate and blood value in newborn intra-uterine growth retricted piglet iv

7 Seion 3: Welfare 13:00-13:15 13:15-13:30 13:30-13:45 13:45-14:00 Karl Johan Møller Klit PhD tudent, Department of Large Animal Science, Univerity of Copenhagen Marlene Kirchner Aitant Profeor, Department of Large Animal Science, Univerity of Copenhagen Helle Pelant Lahrmann Indutrial PhD tudent, Department of Large Animal Science, Univerity of Copenhagen & SEGES Pig Reearch Centre Janni Hale Pederen Pot doc., Department of Large Animal Science, Univerity of Copenhagen 14:00-14:30 AFTERNOON TEA/COFFEE Seion 4: MRSA 14:30-14:45 14:45-15:00 15:00-15:15 15:15-15:30 15:30-15:35 Julie Elvekjær Hanen PhD tudent, DTU National Veterinary Intitute Carmen Epinoa-Gongora Pot doc., Department of Veterinary Dieae Biology, Univerity of Copenhagen Anna Camilla Birkegård PhD tudent, DTU National Veterinary Intitute Nicolai Weber, PhD tudent, Department of Large Animal Science, Univerity of Copenhagen Chritian Fink Hanen Profeor, Department of Large Animal Science, Univerity of Copenhagen Chair: Björn Forkman The ue of virtual herd in veterinary and agricultural education Aeing farm with the Danih animal welfare index Tail biting: prevalence among docked and undocked pig from weaning to laughter Looe houed ow with low piglet mortality Chair: Ander Miki Boyeen Level of MRSA on pig and environmental ample Can we reduce MRSA ST398 in poitive farm by eliminating a few pig carrier? How to pool fecal ample in a croectional tudy of antimicrobial reitance gene in Danih pig herd Can pooled faecal ample be ued for reitance profiling? -Reitance in E. coli iolate from diarrhoeic nurery pig? Concluding remark v

8 Lit of participant Amanda Brinch Krue Anja Annine Haagaard Ana Carolina Lope Antune Andrea Grav Eriken Andrea Birch Andrea Klit Anita Strøm Pederen Anna Camilla Birkegård Anna Helena Stygar Anne Mette Strunz Hanl Anne Schultz Anne Wolfenberg Anne-Charlotte Olon Anne-Helene Tauon Annette Bech Annette Ridderholm Kritenen Arne M Hanen Arhnee Moodley Ager Lundorff Jenen Atrid Larberg Bent-Ole Anderen Birgit Nørrung Birgitte Ak Bjarne Ellegaard Björn Forkman Brian Ekilden Camilla Kaae Højgaard Camilla Sara Birch Sørenen Carmen Epinoa Gongora Caroline Kold Simonen Charlotte Amdi William Charlotte Sonne Kritenen Chiara Trevian Chri Knight Chritian Fink Hanen Chritina Johanen Chritine Lunddahl Clau Fertin Clau Hanen Dagim Belay Dan Jenen PhD tudent, Univerity of Copenhagen DVM, Veterinarian PhD tudent, DTU Vet Svinefagdyrlæge Dyrlæge, Øvet Studerende, Veterinærmedicin Dyrlæge, Fødevaretyrelen PhD tudent, DTU Vet Pot doc., Univerity of Copenhagen, IPH DVM, Hipra Danmark Ap Stud.med.vet., SUND Journalit, L-mediehu Agronomit, Sverige Lantbrukuniveritet Profeor, Univerity of Copenhagen, IKVH Technical Account Manager, Elanco Konulent, DK-Svinerådgivning Konulent, København Univeritet Aociate Profeor, Univerity of Copenhagen Intitutleder, IKVH, SUND, KU PhD tudent, Univerity of Copenhagen Kommunikationmedarbejder, IPH Intitutleder, Int. for Veterinær Sygdombiologi Chefforker, SEGES, VSP-Avl Dyrlæge, MSD AH Profeor, KU Produktudviklingkonulent, Agronom MSc. tudent in Animal Science, Univerity of Copenhagen Studerende, Erhvervakademiet Sjælland Pot doc., Univerity of Copenhagen, IVS Svinerådgiver, LandboNord Pot doc., Univerity of Copenhagen, IPH DVM, SEGES Pig Reearch Centre PhD Student, Univerity of Copenhagen Profeor, UCPH Profeor, Univerity of Copenhagen, IPH Student, Veterinærmedicin Student, Veterinary Medicine Direktør, SEGES Videncenter for Svineproduktion Chefforker, SEGES, Videncenter for Svineproduktion Mr., IFRO PhD tudent, Univerity of Copenhagen vi

9 Danielle Kjerulff Funk Peteren Ebbe Reinhold Nielen Eline Palm Hanen Eliabeth Okholm Nielen Erik Laren Eben Ø. Eriken Flemming Thorup Franzika Hakanon Fredrik Engtröm Gianluca Mazzoni Gitte Blach Nielen Haja Kadarmideen Hanne Juteen Hanne Kongted Han Henrik Dietz Han Houe Han Aae Heidi Brik Helle D Kjærgaard Helle Palmø Helle Pelant Lahrmann Henrik Bech Pederen Henrik Bæktrøm Hugo Holm Inge Laren Inger Morthort Møller Janni Hale Pederen Jen Johanne Sørenen Jen Legarth Jen Noegaard Jørgenen Jen Peter Nielen Jen Ulrich Nielen Jen-Erik Zerrahn Jeper Poulen John Laren Jo Boterman Joefine Lindegaard Joephine Rickert Lundberg Juan Miguel Peralvo Vidal Julie Elvekjær Hanen Student, Animal Science Technical Sale Manager, NordicBiochem Zuattoffe Handel - und Produktion ge.mbh MSc. in Paraitology, PhD tudent, IVS, KU Dyrlæge, Fødevaretyrelen Formand, Landbrug & Fødevarer, Svineproduktion Vet tudent & part time pig farmer, København Univeritetet & Landmand Martin Molbo Chefforker, SEGES Videncenter for Svineproduktion MSc, Copenhagen Univerity/ MSc Applied Ethology and Animal Biology Veterinär, Går & Djurhälan AB PhD tudent, Univerity of Copenhagen Indutrial PhD Student, Univerity of Copenhagen, IPH & MSD Animal Health Profeor, Univerity of Copenhagen Cand. Agro, Erhvervakademi Sjælland Vet, SEGES Pig Reearch Centre Head of Department, IPH, SUND, Univerity of Copenhagen Profeor, Univerity of Copenhagen Group R&D Director, DLG Videnkabelig Aitent, KU Scandinavian Livetock Buine Development manager, Orion Pharma /DVM, MBA, Certificate Swine Health Manager Specialkonulent, NaturErhvervtyrelen - GUDP Indutrial PhD Student, Univerity of Copenhagen & SEGES Pig Reearch Centre Konulent, Merial Norden Afd chef, Tican Freh Meat A/S Direktør, Big Dutchman Skandinavien A/S Dyrlæge, IDT Biologika Kandidattuderende, Veterinær medicin Pot doc., Univerity of Copenhagen, IPH DVM, Svinepraki DK CEO, European Proteinfermentationexpert AS Global Product Manager, Chr. Hanen A/S Profeor, Univerity of Copenhagen, IPH Director, Innovation, SEGES Pig Reearch Centre Technical ale Manager, Nordic-Baltic, EVONIK / MSc.Agric Seniorkonulent, SEGES Videncenter for vineproduktion Head of diviion, DVFA AgrD, Sverige Lantbrukuniveritet Volontør, SEGES Videncenter for Svineproduktion Studerende, ErhvervAkademi Sjælland, Slagele MSc. tudent in Animal Science, Univerity of Copenhagen PhD tudent, DTU Vet vii

10 Julie Lynegaard Jørgen Lindahl Jørgen M. Wetergaard Karl Johan Klit Karl Kritian Kongted Karl Pederen Katarina Karlon Frich Katarina Nielen Dominiak Kathrine Poll Ken Steen Pederen Kimmie Kyed Lyderik Kirten Jenen Kirten Volmer Laren Kirtine Lindtrøm nielen Kjetil Johanen Kritian Møller Kritine Dich-Jørgenen Lar Bagge Juul Lar Erik Laren Lar Jenen Lar Katborg Lar Kritian Clauen Lar Kuntmann Lar Sangill Anderen Laura Myhill Lea Hübertz Leif Meedom Leif Vraa-Anderen Lene juul Pederen Lene Kritenen Libeth Shooter Liette Poulen Liza Roenbaum Nielen Lola Toltrup Leihardt Louie Bundgaard Louie Krue Jenen Louie Hägg Grønborg Magnu Paulon Margit Andreaen Maja Kobberø Maria Ekilden Marianne Kaier Marie Rama Tamberg BSc, Univerity of Copenhagen, Animal Science DVM cert. pig dieae, Ditriktveterinärerna/Ø-vet Dyrlæge, PhD ADC-Conult PhD tudent, Department of Large Animal Science Speialveterinær, Kjøtt og Fjørfebranjen Landforbund/ Veterinær Profeor, DTU Veterinærintituttet Djurhäloveterinär, Gård & Djurhälan AB PhD tudent, Univerity of Copenhagen Dyrlæge, Orion Pharma animal Health Adm. direktør, pecialdyrlæge, Ø-Vet A/S Student, Mater of Animal Science Technical Account Manager, Elanco / DVM Buine Manager, HIPRA Danmark Studerende, Veterinærmedicin Dyrlæge, LVK Head of ection, DTU VET Reearch aitant, Department of Veterinary Dieae Biology Dyrlæge, Fødevaretyrelen, veterinær Nord Profeor, DTU Veterinærintituttet Veterinary Student Area Manager, Phytobiotic Faglærer, RTS Vilvorde DVM, Huvepharma Nutritionit, HAMLET PROTEIN A/S PhD tudent, Copenhagen Univerity Student, Animal Science Dyrlæge, Huvepharma Dyrlæge, Fødevaretyrelen Senior reearcher, Århu Univerity Dyrlæge, Fødevaretyrelen Afdelingleder, SEGES Videncenter for Svineproduktion Konulent, Danih Crown Profeor, Univerity of Copenhagen DVM, PhD tudent, IPH, Univerity of Copenhagen DVM, PhD, Intitut for Produktiondyr og Hete, KU-SUND Aitant Profeor, Univerity of Copenhagen, IVS Dyrlæge, VeterinærSyd, Fødevaretyrelen Djurhäloveterinär, Gård & Djurhälan AB Technical Manager, Danih Aoc. of the Vet Pharm Indutry Studerende, Veterinærmedicin Lektor, Erhvervakademi Aarhu Studerende, KU Studerende, Animal Science viii

11 Marie Erika Buch Marie Stengaard Jenen Marku Drag Marlene K. Kirchner Martin Ramuen Merete Fredholm Mette Fertner Mette Hillerborg Mette Klarlund Mette Olaf Nielen Mette Peteren Mia Pawlowki Michael Albin Laren Miki Bojeen Mona Lilian Vetbjerg Laren Morten Thomen Nicolai Weber Nicoline Rüdiger Wichmann Niel Frede Bertelen Niel Kjelden Niel-Erik Manniche Niel-Peder Nielen Nil Toft Nina Dam Otten Olga Fredborg Nielen Peter Høgedal Peter Nejum Pil Holm Maagaard Pingping Jiang Poul Bækbo Preben Mortenen Rie Jenen Rikke Olen Rikke Søgaard Sara Hanborg Ramuen Simon Smed Sørenen Sine Jakoben Sofie Kromann Sophie Van Vliet Stella Nielen Sundar Thapa Suraya Binti Mohamad Salleh Sven Erik Lind Joral Svend Haugegaard Dyrlæge, SEGES Videncenter for Svineproduktion Cand. cient., DTU Food PhD tudent, IPH KU DVM, PhD, IPH, KU Veterinærtuderende Profeor, KU PhD tudent, Veterinærintituttet Dyrlæge, Ceva Animal Health Student, Veterinary medicin Profeor MSO, Univerity of Copenhagen PhD tudent, Univerity of Copenhagen Dyrlæge, SEGES Videncenter for Svineproduktion DVM, Merial Norden Profeor, Univerity of Copenhagen MSc in Agrobiology, Aarhu Univerity, PhD fellow Journalit, LandbrugMedierne PhD Student, Univerity of Copenhagen, IPH Studerende, Veterinærmedicin DVM, ScanVet Animal Health Senior Specialit, SEGES Videncenter for Svineproduktion Dyrlæge, PHARManniche Deputy Chief, SEGES Videncenter for Svineproduktion Profeor, DTU Veterinærintituttet Adjunkt, IPH KU SUND Studerende, København Univeritet Dyrlæge, LVK Aociate Profeor, Univerity of Copenhagen Dyrlæge, Fødevaretyrelen, Veterinær Nord Pot doc., Univerity of Copenhagen Chef konulent, SEGES Videncenter for Svineproduktion DVM, Merial Norden Cand. Scient in Animal Science, Copenhagen Univerity Aitant profeor, Copenhagen Univerity Dyrlæge, Hipra Danmark Student, Mater of Animal Science Stud. med. Vet, KU Dyrlæge, Øvet Stud.Med.Vet, KU PhD Student, Aarhu Univerity Cand. Scient. Animal Science, PhD tudent, IVS-SUND Univerity of Copenhagen Veterinary Conultant, DTU Veterinary Intitute Vet, SEGES Pig Reearch Centre ix

12 Søren Sloth Thea Kirkebæk Laren Thoma Sønderby Bruun Thoma Thymann Tina Birk Jenen Trine Nyborg Vetergaard Uffe Chritian Braae Vibe D Anderen Vibe Pederen Lund Vivi Aaretrup Mouten Svinerådgiver, LandboNord Cand.cient i agrobiologi, Baltic Control Senior Specialit, SEGES Pig Reearch Centre Aoc. Prof., Univerity of Copenhagen Veterinarian, Danih Veterinary and Food Adminitration Dyrlæge, Fødevaretyrelen, Veterinær Nord PhD tudent, Univerity of Copenhagen PhD tudent, DTU Food Reearch Aitant, Univerity of Copenhagen Senior Scientit, SEGES Pig Reearch Centre x

13 Key Lecture: Benefit of linking univeritie and the Danih pig indutry from the indutry point of view By Jen Ulrich Nielen, Director, Innovation, SEGES Pig Reearch Centre 1

14 BENEFITS OF LINKING UNIVERSITIES AND THE DANISH PIG INDUSTRY FROM THE INDUSTRY S POINT OF VIEW SEGES Pig Reearch Centre Jen Ulrich Nielen, Director, Innovation 1... PRODUCTION OF PIGS IN DENMARK Million pig Production of weaner Export 15.4 million Slaughtering Danih pork export Tonne

15 Global export no leftover China Germany Eatern Europe Japan UK Japan US (rib) Italy Sweden UK France Germany China China China China Gap 0.5 million tonne 5 CONSUMER PRIORITIES

16 DKK/kg COST PER KG CARCASS ,0 13,5 13,0 12,5 12,0 11,5 11,0 10,5 10,0 9,5 9,0 8,5 8,0 NEW PRODUCTION METHODS BASIS OF ADDED VALUE IN THE FUTURE Digitalization of the entire value chain Data/Realtime data a the bai of documentation, production monitoring, deciion upport, benchmarking Emiion-baed environment and climate production Biotechnology (bioga, ethanol, new protein ource etc.) Cot-efficient environmental technology Production concept adapted to pig producer condition and to the future tructure 8... NEW URGENT NEEDS FOR RESEARCH Big data Data-driven innovation Internet of thing in production technology Reduction in ue of antibiotic and zinc

17 Tonne ANTIBIOTIC USE FOR PIGS million weaner 32 million weaner 50 0 Year Therapeutic Growth promoter ZINC AND COPPER IN SLURRY AND SOIL Report from DCE - Danih Centre for Environment and Energy Significant increae in zinc level in oil Rik of future environmental and health problem Miniter for the Environment and Food: more reearch neceary Support reduction baed on cientific ground Reearch in reduction of zinc in feed Reearch in antibodie TWO-LEVEL ADVISORY SYSTEM Reearch CONTINUES Univerity COOP HAS BEEN AND WILL BE THE KEY TO SUCCESS Innovation DIRECT information (Congre, eminar, webite etc.) Advior DLBR (Danih Agricultural Adviory Service) Vet Practice Companie (laughterhoue, feedtuff, farm technology etc.) Pig producer 5

18 DANISH PIG RESEARCH CENTRE Financed by Danih pig farmer 160 employee Reponible for reearch and development programme and knowledge tranfer to the Danih pig indutry Support the development of a reponible and economically utainable pig indutry with the highet poible level of welfare and a minimum impact on the environment Trial for 15 million Euro annually Operate on 200 commercial farm STRONGHOLDS IN COOPERATION WITH THE UNIVERSITIES Enure that the indutry benefit from reearch More relevant pig reearch Involvement of central qualification Improve credibility and validation of SEGES PRC work Acce to more fund Knowledge tranfer to the indutry Supplementary training of employee Recruitment of new employee Mutual uality aurance Mot recent knowledge included in education MANY CHALLENGES - LOTS OF RESEARCH WE ARE LOOKING FOR ANSWERS AND SOLUTIONS, NOT COSTS ow with gatric ulcer Routine tail docking Catration High ow mortality rate Environmental impact MRSA Antibiotic ue 6

19 Seion 1: Growing Pig 7

20 8 PCV2 infection dynamic: Diagnotic, between-batch conitency and correlation to productivity parameter By Gitte Blach Nielen, Indutrial PhD tudent, Department of Large Animal Science, Univerity of Copenhagen & MSD Animal Health

21 UNIVERSITY OF COPENHAGEN Faculty of Health and Medical Science PCV2 infection dynamic: Diagnotic, between-batch conitency and correlation to productivity parameter Preliminary reult CPH Pig February 3rd 2016 Gitte Blach Nielen Indutrial PhD tudent, DVM, Certificate in Swine Health and Management Department of Large Animal Science, Section for Animal Welfare and Dieae Control MSD Animal Health, Swine Supervior: Han Houe, Jen Peter Nielen, John Haugegaard UNIVERSITY OF COPENHAGEN Background Porcine circoviru, type 2 (PCV2) known worldwide a a caue of reduced productivity in growing pig Not killing pig for diagnoi blood ample golden tandard Impact of infection: Level of viremia in erum by PCR-analyi Oral fluid ampling quick, more animal, welfare 2 UNIVERSITY OF COPENHAGEN Reearch quetion Doe level of PCV2: Correlate between erum and oral fluid? Vary between batche in the ame herd? Correlate to productivity parameter and antibiotic uage? Sub-dataet from vaccination field trial Only non-vaccinated pig included vaccination influence PCV2 level 3 9

22 UNIVERSITY OF COPENHAGEN Study deign 1 finihing herd Blue SPF highet health tatu 14 batche included (arriving 2 week apart), 2-5 pen ampled in each: 4 blood ample per pen -> 1 pool for PCV2-PCR-analyi 2 cotton rope for oral fluid collection per pen -> 1 pool for PCV2-PCR-analye PCV2-PCR at DTU-Vet. -> viral copie per ml ample on a log(10)-cale Timeline Arrival 2 week 30 kilo 1. 3 week 2. 3 week 3. 3 week 4. Slaughter 110 kilo The ame 4 pig bled at conecutive ampling time point unle removal due to death or dieae had occurred 4 UNIVERSITY OF COPENHAGEN Reult Totally, 65 pen ampled 4 time = 260 erum/oral fluid pair Reult are not publihed yet and therefore omitted 5 UNIVERSITY OF COPENHAGEN Diagnotic erum and oral fluid correlation Reult are not publihed yet and therefore omitted 6 10

23 UNIVERSITY OF COPENHAGEN PCV2 infection dynamic within batch Timeline Arrival 2 week 30 kilo 1. 3 week 2. 3 week 3. 3 week 4. Slaughter 110 kilo 7 UNIVERSITY OF COPENHAGEN UNIVERSITY OF COPENHAGEN Between-batch conitency Viremia over time ~ average of the 4 time point 9 11

24 UNIVERSITY OF COPENHAGEN Correlation PCV2 level and other parameter Reult are not publihed yet and therefore omitted 10 UNIVERSITY OF COPENHAGEN Key point for the pig indutry Baed on the reult from thi tudy: Oral fluid ampling eem to be a relevant alternative to blood ampling The prevalence of poitive wa higher The level wa higher Serum level differed between batche (ection) within a herd, oral fluid level did not PCV2 level wa poitively correlated to mortality and antibiotic uage 12

25 A multivariate dynamic linear model for early warning of diarrhea and pen fouling in laughter pig By Dan B. Jenen, PhD tudent, Department of Large Animal Science, Univerity of Copenhagen 13

26 Department of Large Animal Science A multivariate dynamic linear model for early warning of diarrhea and pen fouling in laughter pig Dan B. Jenen daj@und.ku.dk Centre for Herd-oriented Education, Reearch and Development, Department of Large Animal Science, Univerity of Copenhagen CPH PIG 2015 Dia 1 (Early) warning what the point? Department of Large Animal Science 1. So many pig, o little time! A modern farm typically contain thouand of pig Health i aeed by farm taff walking through the herd Problem are eay to mi 2. Senor: alway vigilant! 24/7 enor monitoring combined with detection algorithm Identification of high rik pen: focued attention Proactive repone 3. We need to know what to look for! Some data are more valuable than other CPH PIG 2015 Dia 2 In Concluion Take Home Meage Department of Large Animal Science CPH PIG 2015 Dia 9 1. Automatic detection and early warning can help the farmer focu their attention on high rik pen 2. Drinking behavior and Temperature hold the mot ueful information for predicting diarrhea and pen fouling 3. Different data type might be bet exploited in different way (e.g. ummarie v. modeling) Acknowledgement: Council for Strategic Reearch Pig Reearch Center (VSP) The technical taff at Aarhu Univerity 14

27 Material and method: Multivariate Dynamic Linear Model a Quick Introduction 1. Feature: Dynamic, i.e. Adaptive Provide one-tep-ahead forecat Multivariate: co-variance are conidered! 2. Uefulne: Monitoring of (production) ytem over time Department of Large Animal Science 3. Multiple variable multiple forecat error: Forecat error unification (Choleky decompoition/tranformation) I call it the DLM/Choleky method CPH PIG 2015 Dia 4 Reult: Unified forecat error Healthy batch Unified forecat error Department of Large Animal Science CPH PIG 2015 Dia 5 Adjuting SE and SP: How many conecutive alarm for one full alarm? Reult: Performance evaluation Department of Large Animal Science CPH PIG 2015 Dia 6 Prediction window: -3/+1 day 15

28 Reult: Performance evaluation Department of Large Animal Science CPH PIG 2015 Dia 7 Prediction window: -3/+1 day Reult: Performance evaluation - previouly Department of Large Animal Science Temperature alone, Summarie/logitic reg.: AUC = 0.80 CPH PIG 2015 Dia 8 Prediction window: -3/+1 day Temperature alone, DLM/Choleky: AUC = 0.55 In Concluion Take Home Meage Department of Large Animal Science CPH PIG 2015 Dia 9 1. Automatic detection and early warning can help the farmer focu their attention on high rik pen 2. Drinking behavior and Temperature hold the mot ueful information for predicting diarrhea and pen fouling 3. Different data type might be bet exploited in different way (e.g. ummarie v. modeling) Acknowledgement: Council for Strategic Reearch Pig Reearch Center (VSP) The technical taff at Aarhu Univerity 16

29 Oteomyeliti in Danih laugther pig By Louie Krue Jenen, Aitant Profeor, Department of Veterinary Dieae Biology, Univerity of Copenhagen 17

30 Oteomyeliti in Danih laugther pig Louie Krue Jenen, DVM, PhD Aitant profeor in Veterinary Pathology Oteomyeliti i a common finding in Danih laughter pig. Welfare problem for the indiviual pig. Economic problem Inflammation of the bone and bone marrow = oteomyeliti 18

31 Pathogenei Sytemic haematogenou Metaphyi long bone Vertebrae Rib Local lymphatic/haematogenou Vertebrae, caudally from o acrum Tail biting Traumatic Shoulder ulceration Concomitant tail biting and vertebral oteomyeliti in and caudally from o acrum = local rejection of the pelvic bloc Department of Veterinary Dieae Biology 19

32 Sequelae of oteomyeliti Pathological fracture Arthriti Soft tiue/mucular abce Sequetra formation Retained grow Malformation of the bone Incidence of oteomyeliti Approved for deboning Pyaemia Approved for deboning Kg BW >110 Kg BW Pyaemia Why i thee data a problem? Economic 20

33 Animal welfare My reearch PhD title: Development, charaterization and application of a porcine model for haematogenou oteomyeliti in children The fingerprint of oteomyeliti A model i a lie that help you ee the truth Analyi of bone tiue and blod ample for pecific biomarker of oteomyeliti (Immunohitochemitry, microrna, mrna) 21

34 Benefit of my reearch for the Danih pig indutry Finding of biomarker for oteomyeliti can reult in a futher diagnotic tool Increaed focu on individual welfare 22

35 Monitoring growth in finiher by weighing elected group of pig By Anna Helena Stygar, Pot doc., Department of Large Animal Science, Univerity of Copenhagen 23

36 Department of Large Animal Science Monitoring growth in finiher by weighing elected group of pig Anna Helena Stygar Department of Large Animal Science Univerity of Copenhagen CPH Pig Data on body weight Knowledge about the herd (previou growth record) Inertion body weight (at batch, pen and animal level) Monitoring elected group of pig (at pen and animal level) CPH Pig Slide 2 Department of Large Animal Science Department of Large Animal Science Why to monitor body weight? Production control Delivery trategy CPH Pig Slide 3 From Kure,

37 Department of Large Animal Science Objective of thi tudy Develop a monitoring and deciion upport tool Production control Marketing deciion (forecating number of pig above a body weight threhold) Quantify the value of information in finiher (including or excluding the information on body weight) CPH Pig Slide 4 Material and method data from the herd Batche: 9 Data collected between: BW obervation at inertion and firt delivery (all pig) BW obervation of elected group (every week of 2 double pen) Total number of obervation: 9,800 Number of obervation ued for etting model parameter:7,918 Number of obervation ued for teting: 1,882 Number of oberved pen in a batch: 14 Number of pig inerted: ~480 CPH Pig Slide 5 Department of Large Animal Science Material and method data from the herd CPH Pig Slide 6 Department of Large Animal Science 25

38 Material and method parameter etimation + +( + + )t +( + + Department of Large Animal Science Animal effect CPH Pig Slide 7 Material and method Multivariate Dynamic Linear model and Kalman filter Department of Large Animal Science Obervation equation:, Sytem equation:, CPH Pig Slide 8 Material and method Multivariate Dynamic Linear model and Kalman filter Prior: One tep forecat: Poterior: Sequential forecat for k tep ahead for j=1,,k: Forecat ditribution: CPH Pig Slide 9 Department of Large Animal Science 26

39 Reult: production control at batch and pen level Department of Large Animal Science CPH Pig Slide 10 Reult: production control batch comparion CPH Pig Slide 11 Department of Large Animal Science Reult: predicting the number of pig ready for marketing CPH Pig Slide 12 Input: Initial herd information Number of BW meaurement: 0 Department of Large Animal Science 27

40 Reult: predicting the number of pig ready for marketing Department of Large Animal Science Input: Initial herd information and inertion BW of all pig Number of BW meaurement: 486 CPH Pig Slide 13 Reult: predicting the number of pig ready for marketing Input: Initial herd information, inertion BW of all pig and elected group monitoring (15%) Number of BW meaurement: CPH Pig Slide 14 Department of Large Animal Science Reult: predicting the number of pig ready for marketing Input: Initial herd information, inertion BW of all pig and elected group monitoring (15%) Number of BW meaurement: CPH Pig Slide 15 Department of Large Animal Science 28

41 Reult: predicting the number of pig ready for marketing Department of Large Animal Science Input: Initial herd information, inertion BW of all pig and elected group monitoring (15%) Number of BW meaurement: ~ CPH Pig Slide 16 Reult: predicting the number of pig ready for marketing CPH Pig Slide 17 Department of Large Animal Science MAD=1.6 pig CV = 24% Reult: predicting the number of pig ready for marketing CPH Pig Slide 18 Department of Large Animal Science MAD=3.6 pig CV = 102% 29

42 Reult: predicting the number of pig ready for marketing Department of Large Animal Science Oberved CPH Pig Slide 19 Reult: predicting the number of pig ready for marketing CPH Pig Slide 20 Department of Large Animal Science Oberved Concluion We have a tool (production control, delivery trategy). The tool wa able to combine information from different ource (BW information from different pen and pig) and to increae the preciion of knowledge. When a detailed level of information wa provided, the forecat on number of pig above given threhold were given with high accuracy and preciion. Aggregation (to pen level) only lightly reduced the accuracy of prediction. Data on initial BW had high value for the prediction procedure. CPH Pig Slide 21 Department of Large Animal Science 30

43 Concluion practical application Department of Large Animal Science Model can be ued for production control. Model can be ued to inform a farmer about the tarting week of the delivery a well a number of pig ready to market from a given pen. Further economic evaluation i neceary!! CPH Pig Slide 22 Department of Large Animal Science Acknowledgment: Thi reearch wa upported by the Danih Council for Strategic Reearch (The PigIT project, Grant number ) Project manager: Profeor Ander Ringgaard Kritenen CPH Pig Slide 23 31

44 32

45 Seion 2: Sow and Piglet 33

46 34

47 Monitoring PRRS uing laboratory data By Ana Carolina Antune, PhD tudent, DTU National Veterinary Intitute 35

48 Monitoring PRRS uing laboratory data Ana Carolina Antune*, Fernanda Dorea, Dan Jenen, Tariq Halaa and Nil Toft * What i Porcine Reproductive and Repiratory Syndrome (PRRS)? Some general information Caued by a viru Clinical ymptom: it varie a lot! Endemic in Denmark Serology tet performed on regular bai (SPF ytem) 2 DTU Vet, Technical Univerity of Denmark 03/02/2016 Jut a quick overview of the data How often are teted? Montly Yearly 3 DTU Vet, Technical Univerity of Denmark 03/02/

49 What are the objective? Monitor PRRSV in Danih wine herd PRRS eroprevalence Univariate proce control algorithm Dynamic generalized linear model Arinna Comin 4 DTU Vet, Technical Univerity of Denmark 03/02/2016 How can thi be ued? Set up a monitoring ytem Dieae outbreak detection Evaluate control and eradication program Extended to other dieae and animal pecie Extended to other databae Combined with coordinate (patiotemporal analyi) 5 DTU Vet, Technical Univerity of Denmark 03/02/2016 Thank you for your attention 6 DTU Vet, Technical Univerity of Denmark 03/02/

50 38

51 Impact of feeding regime on growth in prepubertal gilt By Sophie Van Vliet, PhD tudent, Aarhu Univerity 39

52 AU IMPACT OF FEEDING REGIME ON GROWTH IN PREPUBERTAL GILTS Sophie van Vliet 1, Thoma S. Bruun 2, Janni Hale Pederen 3, Chritian Fink Hanen 3, Peter K. Theil 1 1 Aarhu Univerity 2 SEGES Pig Reearch Centre 3 Univerity of Copenhagen Sophie.vanvliet@au.dk AARHUS UNIVERSITY BACKGROUND Currently mot gilt are not fed according to their requirement Overall aim: Reduce feed conumption in gilt/ ow without negatively affecting production and longevity, by increaing body fat retention and reducing body protein retention in growing gilt prior to mating Objective: Manipulate growth and body compoition in gilt (Long term conequence of altered body compoition - colotrum and milk yield) AU AARHUS UNIVERSITY 2 EXPERIMENT 3 treatment 48 gilt 3 littermate from 16 litter 60 kg LW (~15 week old) Fed according to bodyweight Meaurement Weight and backfat every econd week Blood ample in week 0, 4, 8 and 12 D 2 O enrichment in week 0 (n=9) and at firt heat after 25 week of age (all) AU AARHUS UNIVERSITY 3 40

53 Feed intake, kg/ d TREATMENTS LPAD low protein ad libitum (4.1/ 3.3 g SID Ly/ FU ~ diet for pregnant ow) High fat depoition intermediate protein depoition MPRE moderate protein retricted (5.0/ 4.1 g SID Ly/ FU) Low fat depoition low protein depoition HPAD high protein ad libitum (6.6/ 5.0 g SID Ly/ FU ~ diet for laughter pig) Intermediate fat depoition high protein depoition AU AARHUS UNIVERSITY 4 FEED INTAKE 4,0 3,5 3,0 Treatment ***; Week ***; TxW *** 2,5 2,0 1,5 1,0 0,5 0, Week LPAD MPRE HPAD AU AARHUS UNIVERSITY 5 THREE LITTERMATES Week 12 AU AARHUS UNIVERSITY 6 41

54 Backfat, mm Live weight, kg Weight gain, g/ d WEIGHT GAIN Treatment ***; Week ***; TxW ** Week LPAD MPRE HPAD AU AARHUS UNIVERSITY 7 LIVE WEIGHT Treatment ***; Week ***; TxW *** Week LPAD MPRE HPAD AU AARHUS UNIVERSITY 8 BACKFAT Treatment ***; Week ***; TxW *** Week LPAD MPRE HPAD AU AARHUS UNIVERSITY 9 42

55 BODY FAT AND PROTEIN POOLS Kg Initially Kg Firt heat a 27.9 b 37.3 a Fat Protein y 19.5 z 23.1 x 0 LPAD MPRE HPAD 0 LPAD MPRE HPAD Treatment *** AU AARHUS UNIVERSITY 10 PLASMA METABOLITES Treatment LPAD MPRE HPAD P-value Glucoe, mm Lactate, mm 3.01 a 3.02 a 2.34 b 0.03 Urea, mm 3.91 a 3.40 b 3.50 b <.001 NEFA, µm 27.7 b 28.0 b 34.1 a Triglyceride, mm 0.31 b 0.33 b 0.37 a <.001 Inulin, pm 26.0 a 17.8 b 31.2 a <.001 IGF-1, ng/ ml 147 b 144 b 168 a AU AARHUS UNIVERSITY 11 CONCLUSION Body fat and protein retention can be altered by feeding regime LPAD high fat retention and intermediate protein retention MPRE low fat and protein retention HPAD high protein retention and intermediate fat retention Follow up tudy -> the effect of altered body compoition on colotrum and milk yield AU AARHUS UNIVERSITY 12 43

56 THANK YOU FOR YOUR ATTENTION AU AARHUS UNIVERSITY 13 44

57 Increaing the dietary level of protein for lactating ow affect litter gain and ow weight lo By Thoma Sønderby Bruun, Senior Specialit, SEGES Pig Reearch Centre 45

58 Increaing the dietary level of protein for lactating ow affect litter gain and ow weight lo Thoma Sønderby Bruun, SEGES Pig Reearch Centre & Anja V. Strathe, Department of Large Animal Science Background Sow are genetically different today Heavier when fully grown Larger litter ize Le body fat content Increaed number of weaned piglet per weaning Poibly increaed milk yield Reduce ow feed cot Studie of protein requirement for lactating ow are needed Aim Finding optimal protein concentration in feed for lactating ow Maximize daily gain of the litter Moderate weight lo for the ow Material and method Feed compoition Treatment Energy FUow per kg SID lyine g per FUow SID crude protein g per FUow Black = planned Pink = realized

59 Sow body weight lo, kg/day Litter weight gain, kg/day Material and method 540 ow tudy Placement Day 2 Weaning Sow body weight Sow backfat Litter equaliation 14 piglet/ow Litter weight Sow body weight Sow backfat Litter weight Sow body weight Sow backfat Reult Average daily litter gain 4 Kuld tilvækt, kg/dag Ly / FEo SID crude protein per FUow Reult Sow weight lo 1 Vægt tab ho oen, kg/dag % cl OBS Etimate SID crude Ly protein / FEo per FUow 47

60 Reult Subequent reproduction No effect on the number of day to firt mating No effect on farrowing rate Marginal effect on litter ize Material and method 72 ow tudy Day 3 Day 10 Day 17 Day 24 Milk Body compoition Milk Litter weight Milk Litter weight Sow body weight Sow backfat Body compoition Reult Le weight lo in lat week of lactation Vægttab d17-25, kg Weight lo, kg g/fuow St. Ford. råprotein, g/feo SID crude protein, g/fuow 48

61 Reult Litter weight gain week 3 Kuldtilvækt d17-25, kg/d Average daily litter gain, kg/day 129 g/fuow St. SIDFord. crude råprotein, g/fuowg/feo Reult Increae in protein decreae in mobilization Tab af kropprotein, kg Body protein lo, kg St. SID Ford. crude råprotein, g per g/feo FUow Reult Sow milk compoition Milk fat, % Fedt mælk, % Milk protein, % Protein mælk, % 5, ,5 131 g/fuow SID crude St. Ford. protein, råprotein, g/fuow g/feo SID crude St. Ford. protein, råprotein, g/fuow g/feo 49

62 Concluion Increae protein during lactation Increaed litter weight gain Reduced ow body weight lo Limited mobilization of mucle protein Increaed mobilization of fat Increaed nutritional content in milk (No influence on prevalence of piglet diarrhea) Subequent reproduction wa not affected Minor poitive effect of increaed protein on ubequent litter ize Thank you for your attention! 50

63 Gatric emptying rate and blood value in newborn intra-uterine growth retricted piglet By Charlotte Amdi William, Pot doc., Department of Large Animal Science, Univerity of Copenhagen 51

64 Department of Large Animal Science Gatric emptying rate and blood value in newborn intra-uterine growth retricted piglet Charlotte Amdi William Ph.d., Pot doc, Univerity of Copenhagen, Department of Large Animal Science CPH Pig 03/02/16 Dia 1 Intrauterine growth retricted piglet Hyperprolific ow intrauterine growth retricted (IUGR) piglet Impaired growth and development during getation Poor maternal nutrition or lack of adequate oxygen upply to the fetu Department of Large Animal Science CPH Pig 03/02/16 Dia 2 Intrauterine growth retricted piglet More nutrient are redirected to brain and heart fetal adaptive reaction (Roza et al., 2008) 25 % to 30 % of newborn piglet in DK uffer from IUGR when defined on their headhape (Hale et al., 2013, Amdi et al., 2013) Higher mortality rate in IUGR piglet (Hale et al., 2013) Department of Large Animal Science CPH Pig 03/02/16 Dia 3 52

65 Gatric emptying rate, % Intrauterine growth retricted piglet Characteritic: Steep dolphin-like forehead Bulging eye Hair with no direction of growth Department of Large Animal Science Brain a % of body weight at 24 h Hale et al., (2013), JAS Body weight at 24 h Amdi et al., (2013), JAS CPH Pig 03/02/16 Dia 4 The IUGR piglet problem? They can not diget the amount of recommended colotrum (Amdi et al., 2013) Up to 50 % of piglet that die within the firt few day have empty tomach (Hale et al., 2013) Therefore we invetigated: How quickly do the tomach empty? 48 piglet 24 IUGR, 24 Normal Tube-fed 12 ml/kg porcine colotrum at birth Euthanied after 15, 30, 60 and 120 min CPH Pig 03/02/16 Dia 5 Department of Large Animal Science Gatric emptying rate Normal -IUGR Time after tube-fed colotrum Department of Large Animal Science Group: p=0.129 Time: p<0.001 Group x time: p=0.871 Preliminary reult: Amdi, Klarlund et al., in manucript preperation CPH Pig 03/02/16 Dia 6 5 cm Normal IUGR 3 cm 53

66 Blood value Whole blood glucoe, mmol/l No difference between group Difference over time CPH Pig 03/02/16 Dia 7 Normal -IUGR Department of Large Animal Science Group: p=0.146 Time: p<0.001 Group x time: p= Time after tube-fed colotrum Preliminary reult: Amdi, Klarlund et al., in manucript preperation Rectal temperature (influence urvival) Rectal temp. C CPH Pig 03/02/16 Dia 8 Normal -IUGR Department of Large Animal Science Time after tube-fed colotrum Difference between group and over time Drop in temp during the firt 15 min. Group: p< Time: p=0.001 Group x time: p= Difference of 1.3 C in rectal temperature Preliminary reult: Amdi, Klarlund et al., in manucript preperation The IUGR piglet problem? How often can we give IUGR piglet colotrum? 4 group of IUGR piglet: With ow with colotrum With ow without colotrum Without ow with colotrum Without ow without colotrum How much of an effect doe the ow have? Additional heat? Additional colotrum? Tube-fed 12 ml/kg colotrum at birth Weighed Rectal temp Department of Large Animal Science CPH Pig 03/02/16 Dia 9 54

67 Glucoe, mmol/l Glucoe level in IUGR piglet over 8 hour Med o med colo Med o uden Uden o med colo Uden o uden 4,0 3,5 3,0 2,5 2,0 1,5 1,0 0,5 Department of Large Animal Science 0, Time after tube-fed colotrum CPH Pig 03/02/16 Dia 10 Preliminary reult Jenen 2015 Rectal temp, C Rectal temperature in IUGR piglet over 8 hour Department of Large Animal Science Med o med colo Med o uden Uden o med colo Uden o uden Time after tube-fed colotrum CPH Pig 03/02/16 Dia 11 Preliminary reult Jenen 2015 Take home meage Gatric emptying rate i imilar Department of Large Animal Science Stomach are mall and empty fat IUGR piglet might be more challenged in blood glucoe Colotrum increae rectal temperature with one degree However colotrum ha to be given IUGR piglet after 2 week every hour The ow ha an effect CPH Pig 03/02/16 Dia 12 55

68 Thank you: Pig Levy Fund (Svineafgiftfonden) for upport and funding The group at KU: Prof Chritian Fink Hanen, Pot doc Janni Hale, Phd tud Anja Strathe, peciale tud Mette Verner Klarlund, Laura Lundgaard Jenen and Camilla Højgaard Thank you to Akelygaard for their IUGR piglet and help Department of Large Animal Science And a thank you to Julie Lynegaard and Maiken Engelmann for letting u ue ome of their picture CPH Pig 03/02/16 Dia 13 56

69 Seion 3: Welfare 57

70 58

71 The ue of virtual herd in veterinary and agricultural education By Karl Johan Møller Klit, PhD tudent, Department of Large Animal Science, Univerity of Copenhagen 59

72 The ue of virtual herd in veterinary and agricultural education Karl Johan Møller Klit DVM, Phd-Student, Camilla Kirketerp Nielen DVM, PhD-Student Department of Large Animal Science Teaching environment Why virtual herd? Tranfer The ability to ue a kill beyond the preent context To be value any kill mut be tranferable to real life 60

73 Aim/Purpoe Farrowing unit Increae Animal Welfare Piglet urvival Farrowing aitance Farrowing fever Piglet environment 61

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77 Thank you Svineafgiftfonden 65

78 66

79 Aeing farm with the Danih animal welfare index By Marlene Kirchner, Aitant profeor, Department of Large Animal Science, Univerity of Copenhagen 67

80 Aeing farm with the Danih animal welfare index Marlene Kirchner Department of Large Animal Science Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 1 What i an animal welfare index? An animal welfare aement evaluate the welfare tate of the animal at that pecific point in time, repreentative for a period The DAW- Index make it poible to tudy the development of animal welfare over time, eg. everal year. Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 2 The project Project run Farrowing ow & piglet, gilt and getating ow, fattening pig Index 1. Baed on exiting regitration (meat control, ue of antibiotic) Index 2. Baed on farm viit, preferred animal baed meaure Index 3. Bet combination of 1 & 2 Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 3 B.Forkman 68

81 Intitut For Produktiondyr og Hete - Dyrevelfærd og Sygdombekæmpele The urvival factor for included indicator Hedonitic definition of animal welfare Validity reliability feaibility Expert round On-farm viit [90 Pig herd] Welfare Quality comparion Recording time max. 1hour DUNCAN, 1996; KNIERIM & WINCKLER, 2009; WHAY ET AL., 2004; WELFARE QUALITY, 2009 Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 4 Which apect of Animal Welfare are covered by DAWIN? 4 Principle of Animal Welfare Good Feeding Good Houing Good Health Appropriate Behaviour Indicator Behavioural Clinical Reource Record Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 5 27 for farrowing ow 22 for weaner and fattener 21 for piglet 28 getating ow and gilt Can change until the final verion! Intitut For Produktiondyr og Hete - Dyrevelfærd og Sygdombekæmpele The urviving indicator included in DAWIN Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 6 69

82 Farrowing ow part 1 Principle (WQ) Indicator Type Good Feeding Body condition core (0/1) Animal-baed Roughage (No/Acce to roughage) Reource-baed Feeding ytem (Non/competitive) Reource-baed Water upply (clean/ufficient) Reource-baed Good Houing Farrowing ytem Reource-baed Farrowing rail Reource-baed Space in farrowing ytem Reource-baed Reting area Reource-baed Ammonia Reource-baed Manure on the body Animal-baed Buriti Animal-baed Panting Animal-baed Nuring ow Animal-baed Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 7 Farrowing ow part 2 Principle (WQ) Indicator Type Good health Hampered repiration Animal-baed Appropriate Behaviour Sted og dato Dia 8 Shoulder wound Integument alteration Vulva leion Prolape Hernia Noe ring Overgrown claw Proper euthanaia Hopital pen Mortality Stereotypie Rooting material Poibility to perform net building behaviour Animal-baed Animal-baed Animal-baed Animal-baed Animal-baed Animal-baed Animal-baed Reource-baed Reource-baed Reource-baed Animal-baed Reource-baed Reource-baed Intitut For Produktiondyr og Hete - Dyrevelfærd og Sygdombekæmpele Weaner and fattener part 1 Principle (WQ) Indicator Type Good Feeding Body condition core Animal-baed Feeding ytem Reource-baed Water upply Reource-baed Good Houing Stocking denity Animal-baed Reting area Reource-baed Cooling Reource-baed Ammonia Reource-baed Slipperine of the floor Animal-baed Manure on the body Animal-baed Panting Animal-baed Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Dia 9 70

83 Weaner and fattener part 2 Principle (WQ) Indicator Type Good health Lamene Animal-baed Integument alteration Animal-baed Tail bite Animal-baed Ear damage Animal-baed Rectal prolape Animal-baed Hernia Animal-baed Twited nout Animal-baed Neurological ymptom Animal-baed Proper euthanaia Reource-baed Hopital pen Reource-baed Mortality Animal-baed Appropriate Behaviour Rooting material Reource-baed Dia 10 Intitut For Produktiondyr og Hete - Dyrevelfærd og Sygdombekæmpele Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control The future of the DAWIN project 2015 Viiting farm Expert weighting of the meaure 2016 Further haping of the indicator Aggregation of on farm meaure Contructing an index COMING SOON!!! DAW - Index 2016 Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Slide 11 Many thank go to... Danih Veterinary and Food Adminitration Louie Holm Copenhagen Univerity Björn Forkman, Han Houe, Anne Marie Michelen, Nina Otten, Søren Saxmoe Nielen, Matt Denwood, Henrik Elvang Aarhu Univerity Jan Tind Sørenen, Tine Rouing Thank you for your attention! mk@und.ku.dk Copenhagen Pig 2016 Marlene K. Kirchner Section for Animal Welfare and Dieae Control Slide 12 71

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85 Tail biting: prevalence among docked and undocked pig from weaning to laughter By Helle Pelant Lahrmann, Indutrial PhD tudent, Department of Large Animal Science, Univerity of Copenhagen & SEGES Pig Reearch Centre 73

86 TAIL BITING: PREVALENCE AMONG DOCKED AND UNDOCKED PIGS FROM WEANING TO SLAUGHTER Helle Pelant Lahrmann, Indutrial Ph.D. Student February 3th 2016 INTRODUCTION EU directive ban routine tail docking More than 95% of European pig are tail docked (EFSA 2007) Etimated 3.1% of Danih pig tail bitten depite the tail docking procedure (D Earth et al., 2014) The conequence of a ceation of tail docking in conventional Danih piggerie are not known Study aim Determine conequence of tail docking ceation on tail biting in a well-managed Danih conventional herd. DEFINITION TAIL BITING & TAIL LESION Tail biting i a behaviour damaging or non-damaging Definition damaging tail biting Pig chew on a pen mate tail reulting in a bloody wound on the tail (Munterhjelm et al. 2013) Damaging tail biting i painful to the pig can develop to uch an extent that the pig looe the majority of the tail increae the rik of infection increae the need for antibiotic treatment may caue death or euthanaia (Krita & Morrion 2004 & 2007) Tail leion i a condition Tail biting Tail leion 74

87 MATERIAL & METHODS Two group +/- tail docking Bite Rite Houing Same pen from weaning to laughter (table group) Docked and undocked pig in different pen pig per pen, mixed gender Daily proviion of ~230 g traw on the floor until 70 kg + two vertical wooden tick per pen If tail biting occurred a Bite Rite wa added and the amount of traw wa doubled Animal LYD pig, catrated male, individually earmarked PEN DESIGN RECORDINGS Every econd week all tail were inpected by a trained technician Recording by tockperon Date tail biting outbreak Antibiotic treatment Dead/euthanized pig Pig removed from pen and caue Abattoir Tail leion comment/condemned Part miing Full length 75

88 Percentage PRELIMINARY RESULTS Undocked Docked Pig, n Pen, n Tail bitten, n Infected tail injury, n 24 - Dead pig, n 30 (3 TB) 37 Hopital pen, n 38 (25 TB) 12 On average 3.6 % of the pig per can had a tail injury In 19 % of the pen per can there were pig with tail injurie On average the firt tail biting incidence wa oberved 45 day after weaning Gender tail bitten pig: 77 gilt, 123 catrated male and 14 unknown PRELIMINARY RESULTS TAIL BITING, 8-80 KG Weight (kg) Pen (n=47) Pig (n= 963) PRELIMINARY CONCLUSIONS Ceation of tail docking in a well managed herd with a high health tatu and low occurrence of tail biting among docked pig: Increaed the rik of tail biting depite low tocking denity from 7-30 kg and traw Increaed the need for hopital pen Did not increae number of dead pig if the tail biting could be topped Abattoir remark underetimated the prevalence of tail bitten pig 76

89 THANK YOU FOR YOUR ATTENTION!!! Challenging tak!... Finiher with intact curly tail 77

90 78

91 Looe houed ow with low piglet mortality By Janni Hale Pederen, Pot doc., Department of Large Animal Science, Univerity of Copenhagen 79

92 Looe houed ow with low piglet mortality Janni Hale Pederen Pot Doc Department of Large Animal Science February 2016 Thi project wa founded by the Danih Centre for Animal Welfare Background Danih indutry 10 % looe houed lactating ow Looe houed ow i a challenge Higher piglet mortality Temporary confinement for 4 day reduce mortality Not all ow have high mortality Identify good ow Do they have a better farrowing proce? Are they more active? Do they perform more pre-lying behaviour? Experiment Video recording of 40 ow Good ow Bad ow Parity 1 and 2 Obervation from end of farrowing to litter equalization 0-1 dead piglet before equalization 2+ dead piglet before equalization Regitration Farrowing duration Looe Confined Potural change Pre-lying behaviour Piglet behaviour 80

93 Potural change, no/hour Piglet mortality, % Total born piglet, no. Piglet mortality Before litter equalization Equalization to day 4 Total born piglet b c a a Looe Confined Looe Confined Good ow Bad ow Duration of farrowing and obervational period Good ow Bad ow P-value Looe Confined Looe Confined Houing Mortality Sow Farrowing duration, min Time to litter equalization, h Sow behaviour potural change 2 1,5 1 0,5 0 Change to lateral poition Change to ventral poition Rolling Looe Confined Looe Confined Good ow Bad ow Good ow Bad ow P-value Lying lateral, min/event Looe Confined Looe Confined Houing Mortality H x M Standing, min/event

94 Percent of obervation Percent of obervation Event per hour Event per hour Event per hour Sow behaviour pre-lying behaviour Lying down away from piglet Rooting or pawing 1 0,8 0,6 0,4 1 0,8 0,6 0,4 0,2 0 Looe Confined Looe Confined Good ow Bad ow 0,2 0 Looe Confined Looe Confined Good ow Bad ow Sow piglet contact 1 a 0,8 0,6 0,4 0,2 a b b 0 Looe Confined Looe Confined Good ow Bad ow Piglet behaviour Piglet location Piglet activity Good ow Bad ow Good ow Bad ow Sow Creep Other Standing/walking Nuring/at the udder Lying elewhere Concluion Poible indicator of low piglet mortality Sow related Duration of farrowing Activity or inactivity Performance of pre-lying behaviour Piglet related Location in pen Activity or inactivity Total born piglet and parity are till the bet indicator of low or high piglet mortality. 82

95 Thank you for your attention!!! 83

96 84

97 Seion 4: MRSA 85

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99 Level of MRSA on pig and environmental ample By Julie Elvekjær Hanen, PhD tudent, DTU National Veterinary Intitute 87

100 Level of MRSA on pig and environmental ample Julie Elvekjær Hanen MSc. in Biology-Biotechnology, PhD tudent Technical Univerity of Denmark, National Veterinary Intitute, Frederikberg, Denmark Introduction Aim Method Method verification Reult Concluding remark LA-MRSA i a public health concern Denmark: overall MRSA level i low Ambition of maintaining low level of MRSA Increaing prevalence in livetock, epecially in pig production LA-MRSA load not known LA-MRSA reervoir poibility of pread into the general population Control and prevention of increaing MRSA level in community Aement of intervention trategie MRSA load? 2 Introduction Aim Method Method verification Reult Concluding remark Aim of the tudy To ae the poibilitie of quantification of the animal MRSA load by naal and kin wab ample To tet two different active air ampler for quantification of airborne MRSA a a meaure of environmental MRSA load 3 88

101 Introduction Aim Method Method verification Reult Concluding remark Quantification of animal load and air load Colony count and determination of colony forming unit count dilution factor C ml wa. m Determination of CFU/m 3 4 Introduction Aim Method Method verification Reult Concluding remark Animal load - Biological and technical replicate Sampling : 3 pig 4x3 wab were obtained a: 3 naal wab + 3 kin wab from each ide of the pig Each of biological replicate were divided and analyed a three technical replicate in the lab, leading to 36 ample from each pig in total Naal wab eem to caue greater variation in the reult than ue of kin wab. For naal wab a larger proportion of the variance can be explained by the difference between pig. 5 Introduction Aim Method Method verification Reult Concluding remark Animal load - Biological and technical replicate Sampling : 3 pig 4x3 wab were obtained a: 3 naal wab + 3 kin wab from each ide of the pig Each of biological replicate were divided and analyed a three technical replicate in the lab, leading to 36 ample from each pig in total Naal wab eem to caue greater variation in the reult than ue of kin wab. For naal wab a larger proportion of the variance can be explained by the difference between pig. 6 89

102 Introduction Aim Method Method verification Reult Concluding remark Environmental load - Airborne LA-MRSA Volume: 50l, 100l, 250l, 500l Sample obtained in duplicate Sartoriu yielded the mot table detection level of LA-MRSA from the different volume and the increaing raw count detected correpond better with increaing air volume. 7 Introduction Aim Method Method verification Reult Concluding remark Level of naal, kin and air load MRSA load in naal wab are generally higher and more variable between pig than MRSA load in kin wab. 8 Introduction Aim Method Method verification Reult Concluding remark Animal load farm level Farm range Lower Upper Sow Gilt Pregnant Farrowing Weaning Slaughter Farm avg. Prevalance 1,42E+04 4,15E+04 1,89E+03 5,00E+00 7,00E+05 1,79E+04 62/64 (97%) Farm 1 Farm 2 4,41E+02 4,48E+03 5,00E+00 2,80E+04 1,34E+03 60/62 (97%) 5,70E+01 5,51E+01 Farm 3 3,83E+03 4,33E+03 6,60E+01 3,00E+04 4,13E+03 25/25 (100%) Farm 4 1,70E+02 2,90E+02 5,00E+00 1,90E+03 1,49E+02 35/43 (83%) 6,43E+00 Farm 5 1,00E+01 5,00E+00 7,50E+00 4,44E+02 5,00E+00 1,87E+03 2,40E+02 17/41 (41%) 9 90

103 Introduction Aim Method Method verification Reult Concluding remark Concluding remark Quantification of animal LA-MRSA load i poible by direct plating Meaurement of airborne LA-MRSA load are more table from Sartoriu MD8 air ampler than Sampl air Limitation - Contaminant growth can interfere - Difficult to tandardize - Air level a a time point meaurement Benefit - Societal knowledge and demytification of wine-mrsa - We hope to be able to reduce or hinder the amount of LA-MRSA that ecape the farm - Provide knowledge for the authoritie to bae their deciion-making on 10 Acknowledgement Main upervior Karl Pederen (DTU Vet) Co-upervior Ander Rhod Laren (SSI) Ulrike Lyh (DTU Vet) DTU Vet SSI Univerity of Copenhagen Anna Irene Vedel Sørenen Robert Leo Skov Carmen Epinoa-Gongora Nil Toft Jeper Laren Margrethe Carlen Øytein Angen Kári Karbech Mouriten 11 91

104 92

105 Can we reduce MRSA ST398 in poitive farm by eliminating a few pig carrier? By Carmen Epinoa Gongora, Pot doc., Department of Veterinary Dieae Biology, Univerity of Copenhagen 93

106 CAN WE REDUCE MRSA ST398 LEVELS IN THE FARM BY REDUCING A FEW PIG CARRIERS? CPH PIG SYMPOSIUM Feb 2016 CARMEN ESPINOSA-GONGORA ceg@und.ku.dk Department of Veterinary Dieae Biology Faculty of Health and Medical Science Univerity of Copenhagen Animal Genetic, Bioinformatic and Breeding Merete Fredholm Clau B. Jørgenen Per Skallerup Veterinary Dieae Biology Luca Guardabai DANISH COUNCIL FOR INDEPENDENT RESEARCH FTP Carmen Epinoa-Gongora STAPHYLOCOCCUS AUREUS COLONIZATION Commenal Carriage/colonization 20% peritent carrier 30% intermittent carrier 50% non-carrier Factor? Bacterial Hot Genetic IR Microbiota Environment General population S. aureu naal carrier 80% PEOPLE WITH SKIN INFECTIONS WERE CARRIERS 65% INFECTIONS caued by COLONIZING STRAIN Wertheim et al, Lancet

107 THE PROBLEM OF MRSA IN PIG FARMING I THE PROBLEM OF MRSA IN PIG FARMING II THE PROBLEM OF MRSA IN PIG FARMING III Directly expoed Indirectly expoed Non-expoed Healthcare environment Farm Houe Community Hopital MRSA control policy 95

108 SUPERCARRIERS SUPERCARRIER A minority of the pig (4-11%) are SUPERCARRIERS characterized by a high amount and table carriage of S. aureu in the noe Epinoa-Gongora et al Appl. Env. Microbiol. ARE HOST GENETICS INVOLVED AS A CARRIAGE FACTOR? SUPERCARRIERS ARE HOST GENETICS INVOLVED AS A CARRIAGE FACTOR? V. Whole genome equencing of the pig GENOME-WIDE ASSOCIATION STUDY (GWAS) GWAS tudy uing Illumina Porcine SNP60 beadchip (Ramo et al. PLoS one 2009) Skallerup et al., Genome-wide aociation tudy reveal a locu for naal carriage of Staphylococcu aureu in Danih crobred pig. BMC Veterinary Reearch Chemokine CCL1 CCL2 CCL8 CCL11 IMMUNE MEDIATORS 96

109 GENOME-WIDE ASSOCIATION STUDY (GWAS) GWAS tudy uing Illumina Porcine SNP60 beadchip (Ramo et al. PLoS one 2009) Skallerup et al., Genome-wide aociation tudy reveal a locu for naal carriage of Staphylococcu aureu in Danih crobred pig. BMC Veterinary Reearch NON-CARRIER 85-95% SUPERCARRIER 5-15% RAPID DIAGNOSTIC BY PCR CONCLUSIONS & FUTURE WORK Reducing MRSA level in pig farm require MULTIPLE meaure FARM TRIAL to tet the efficacy of eliminating the upercarrier In combination with more trategie (antimicrobial ue? zinc? hygiene? probiotic? diinfection method? etc ) Invetigate functional difference in the immune repone poible new trategie 97

110 DANISH COUNCIL FOR INDEPENDENT RESEARCH FTP CARMEN ESPINOSA-GONGORA Department of Veterinary Dieae Biology Faculty of Health and Medical Science Univerity of Copenhagen 98

111 How to pool fecal ample in a cro-ectional tudy of antimicrobial reitance gene in Danih pig herd By Anna Camilla Birkegård, PhD tudent, DTU National Veterinary Intitute 99

112 How to pool fecal ample in a cro-ectional tudy of antimicrobial reitance gene in Danih pig herd Julie Claen, Ander Mellerup, John Elmerdahl Olen, Øytein Angen, Ander Folkeon, Tariq Halaa, Nil Toft, Anna Camilla Birkegård Why? Level of zoonotic antimicrobial reitance gene in Danih pig herd Cro-ectional tudy herd Spatial randomne Short ample period 2 100

113 Where? 3 How many? Minimal number of ample Herd level of antimicrobial reitance 4 15 Farm

114 Creating the pool 20 fecal ample 6 20 individual ample 48 pool Analyzing the ample 7 Level of antimicrobial reitance 8 102

115 Reult 9 Optimizing the pooling method 5 herd 5 ample per herd 3 pooling method 10 pool for method 1 and 2 5 pool for method Pooling method g 9 ml 2 a b c x pool ize 3 a a + x pool ize 3.5 ml b 0.5 ml x pool ize x ml 10 % 103

116 Pooling method 12 Concluion Five ample per herd Pooling method

117 Can pooled faecal ample be ued for reitance profiling? -Reitance in E. coli iolate from diarrhoeic nurery pig By Nicolai Weber, PhD tudent, Department of Large Animal Science, Univerity of Copenhagen 105

118 Department of Large Animal Science Can pooled faecal ample be ued for reitance profiling? -Reitance in E. coli iolate from diarrhoeic nurery pig Nicolai Weber PH.D STUDENT, Department of Large Animal Science, Univerity of Copenhagen Department of Large Animal Science Dia 2 Department of Large Animal Science Hypothei: Virulent E.coli iolate from the pen floor ha ame reitance profile a virulent E.coli iolate from diarrheic nurery pig contained in the pen Dia 3 106

119 Motivation Development of diagnotic deciion tool for election of antibiotic clae for treatment of E.coli diarrhoea Department of Large Animal Science Supervior: Jen Peter Nielen (Profeor, DVM, PhD, Dipl. ECPHM) Chritian Fink Hanen (Aociated Profeor, MSc Animal Science, PhD) Ken Steen Pederen (CEO OE-Vet A/S, DVM, PhD, Dipl. ECPHM) Dia 4 Study deign Pig ample 3 nurery facilitie In pen with >25 % diarrhoea 3 diarrheic pig per pen Pen ample 1 pen floor ample Department of Large Animal Science Dia 5 Pathogenei Department of Large Animal Science Definition: Virulent E.coli = fibriae poitive and toxin poitive Dia 6 107

120 Study deign Department of Large Animal Science Comparion of reitance tatu at pen level Hypothee: Re.Pen = Re.pig Dia 7 Reult 89 virulent iolate analyed all F18 poitive 87/89 hemolytic activity Department of Large Animal Science 22/86 ampled pig 13/31 ampled pen Dia 8 Reult Antimicrobial cla Dia 9 Overall reitance Department of Large Animal Science % reitant DTU-VET * Clinical breakpoint (µg/ml) # Iolate Tetracycline Ampicillin Sulphamethoxazole Trimethoprim Streptomycin Spectinomycin Reitance profile analyed by Senititre ytem * From the national guideline of antibiotic ue 108

121 Department of Large Animal Science Reult % Reitant by Herd Herd 1 Herd 2 Herd 3 Iolate analyed Tetracycline Ampicillin Sulphamethoxazole Trimethoprim Streptomycin Spectinomycin Dia 10 Reult Agreement tudy: Definition: Department of Large Animal Science Re.pig = 1 or more virulent E.coli iolate from 1 or more pig for the pen Re.pen = 1 or more virulent E.coli iolate from 1 pooled pen floor ample Dia 11 Reult Pig level + Virulent - Virulent Total Pen level + Virulent Virulent Total Senitivity = 83.3 %; Specificity= 84.2 % PPV = 76.9 %; NPV = 88.9 % Department of Large Animal Science Dia

122 Reult Agreement of reitance Antimicrobial cla Agreement Tetracycline 10/10 Ampicilline 10/10 Sulphamethoxazole 10/10 Trimethoprim 10/10 Streptomycin 10/10 Spectinomycin 8/10 Comparion of Re.pen and Re.pig wa only poible i 10 pen Dia 13 Department of Large Animal Science Concluion Dia 14 Department of Large Animal Science 10 of 31 pen virulent E.coli iolated in pen and pig Highet prevalence of reitance to Sulpha, TMP and Tetra Herd pecific reitance pattern Excellent agreement between pen reitance and pig reitance Low ample ize Confirmation in larger tudy needed Dia 15 Department of Large Animal Science 110

123 CPH Pig i financially upported by the Pig Levy Fund (Svineaftgiftfonden) Thank you for participating ee you next year!