OIE Reference Laboratory Reports Activities

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1 OIE Reference Laboratory Report Activitie Activitie in 2017 Thi report ha been ubmitted : :28:18 Name of dieae (or topic) for which you are a deignated OIE Reference Laboratory: Brucelloi (Brucella abortu, B. meliteni) Addre of laboratory: National Intitute of Animal Health, Department of Livetock Development(DLD) 50/2 Moo3, Phahonyothin Road (Kaetklang), Ladyao, Chatuchak, Bangkok THAILAND Tel.: to 14 Fax: to 19 addre: monayae@dld.go.th Webite: niah.dld.go.th Name (including Title) of Head of Laboratory (Reponible Official): Dr. Banjong Jongrakwattana, Director of the National Intitute of Animal Health, DLD Dr. Reka Kanitpun, Chief of Immunology Section (Brucelloi Laboratory) Name (including Title and Poition) of OIE Reference Expert: Monaya Ekgatat, DVM, BSc, DMM. Brucelloi Advior to DLD, National Intitute of Animal Health, Department of Livetock Development Which of the following define your laboratory? Check all that apply: Governmental OIE Reference Laboratory Report Activitie,

2 ToR 1: To ue, promote and dieminate diagnotic method validated according to OIE Standard 1. Did your laboratory perform diagnotic tet for the pecified dieae/topic for purpoe uch a dieae diagnoi, creening of animal for export, urveillance, etc.? (t for quality, proficiency teting or taff training) Tet Indicated in OIE Manual (/) Total number of tet performed lat year Indirect diagnotic tet Nationally Internationally RBT 78, SAT - - CFT 1, I-ELISA (bovine erum) 1, I-ELISA (bovine milk) 3, I-ELISA (caprine/ovine erum) 1,411 - FPA 89 - Milk Ring Tet B. cani RSAT/LFIA - - Direct diagnotic tet Nationally Internationally Culture (milk/organ/wab 198/1,128/34 102/18/71 Brucella identification (animal) Brucella pp. PCR/Real time PCR (pecimen) 1/11 1/71 Brucella molecular typing Control of diagnotic antigen e RBT-Ag 20 e RBT 2 e, CFT 1 2 OIE Reference Laboratory Report Activitie, 2017

3 ToR 2: To develop reference material in accordance with OIE requirement, and implement and promote the application of OIE Standard. To tore and ditribute to national laboratorie biological reference product and any other reagent ued in the diagnoi and of the deignated pathogen or dieae. 2. Did your laboratory produce or upply imported tandard reference reagent officially recognied by the OIE? 3. Did your laboratory upply tandard reference reagent (non OIE-approved) and/or other diagnotic reagent to OIE Member Countrie? OIE Reference Laboratory Report Activitie,

4 Type of reagent available Related diagnotic tet Produced/ provide Amount upplied nationally (ml, mg) Amount upplied internationally (ml, mg). of recipient OIE Member Countrie Region of recipient National tandard panel of poitive era reagent Produced 37 vial (37x1.0 ml) - - Aia and Eat National tandard panel of negative era reagent Produced 37 vial (37x1.0 ml) - - Aia and Eat Negative erum (for recontitute) reagent Produced 20 vial (20x5.0 ml) - - Aia and Eat Secondary reference tandard erum (RBT-Bru Bovine Po Std- NIAH) RBT antigen Produced 15 vial (15x1.0 ml) - - Aia and Eat Secondary reference tandard erum (CFT-Bru Bovine Po Std-NIAH) CFT antigen Produced Aia and Eat RBT antigen reagent Provide 1309 bottle (1309x10 ml) - - Aia and Eat 4 OIE Reference Laboratory Report Activitie, 2017

5 MRT antigen reagent Produced Aia and Eat RSAT antigen reagent Produced Aia and Eat PCR (Bruceladder) PCR Produced Aia and Eat Secondary reference tandard goat erum (RBT) reagent Produced Aia and Eat 4. Did your laboratory produce vaccine? 5. Did your laboratory upply vaccine to OIE Member Countrie? ToR 3: To develop, tandardie and validate, according to OIE Standard, new procedure for diagnoi and of the deignated pathogen or dieae 6. Did your laboratory develop new diagnotic method validated according to OIE Standard for the deignated pathogen or dieae? 7. Did your laboratory develop new vaccine according to OIE Standard for the deignated pathogen or dieae? OIE Reference Laboratory Report Activitie,

6 ToR 4: To provide diagnotic teting facilitie, and, where appropriate, cientific and technical advice on dieae meaure to OIE Member Countrie 8. Did your laboratory carry out diagnotic teting for other OIE Member Countrie? Name of OIE Member Country eeking aitance Date (month). ample received for proviion of diagnotic upport. ample received for proviion of confirmatory diagnoe BHUTAN 30 Jan 2017 Serum 121 ample Milk 50 ample Specimen 6 ample - LAOS 24 July 2017 Vaginal wab 71 ample DNA extraction 71 ample - BHUTAN 13 Sep 2017 Serum 178 ample Milk 52 ample Specimen 12 ample - 9. Did your laboratory provide expert advice in technical conultancie on the requet of an OIE Member Country? Name of the OIE Member Country receiving a technical conultancy Purpoe How the advice wa provided PHILIPPINES Serological teting Remote aitance through communication MONGOLIA Laboratory quality Remote aitance through communication PAPUA NEW GUINEA RBT antigen Remote aitance through communication ToR 5: To carry out and/or coordinate cientific and technical tudie in collaboration with other laboratorie, centre or organiation 10. Did your laboratory participate in international cientific tudie in collaboration with OIE Member Countrie other than the own? 6 OIE Reference Laboratory Report Activitie, 2017

7 Title of the tudy Duration Purpoe of the tudy Partner (Intitution) OIE Member Countrie involved other than your country Development of Milk-ELISA kit for detection of antibody againt Brucella pp. 2014/ /03 Comparion of antibody titer between milk and erum of cattle by uing the Brucella ELISA kit which ha been approved in Japan for diagnoi Optimization of preparation milk ample for diagnoi National intitute of Animal Health (NIAH), Japan JAPAN ToR 6: To collect, proce, analye, publih and dieminate epizootiological data relevant to the deignated pathogen or dieae 11. Did your Laboratory collect epizootiological data relevant to international dieae? 12. Did your laboratory dieminate epizootiological data that had been proceed and analyed? 13. What method of diemination of information i mot often ued by your laboratory? (Indicate in the appropriate box the number by category) a) Article publihed in peer-reviewed journal: 0 b) International conference: 6 1. FAO-APHCA/OIE Regional Technical Workhop on the Prevention and Control of Animal Brucelloi and Tuberculoi in Aia. September 11-13, (preentation and participation) 2. Regional Meeting for OIE Reference Center for Aia and the Tokyo, 6-7 February Ueno Y., Kunchit P., Kanitpun R., Yamamoto T., Yamaguchi E., Kulorn S., Ekgatat M. and Oaki M. 2017: Evaluation of milk ample for diagnoi of bovine brucelloi with Japanee enzyme-linked immunoorbent ay (ELISA) kit. The 5th Thailand-Japan Joint Conference on Animal Health. National Intitute of Animal Health, Bangkok, Thailand, June 6-8, (Oral preentation). 4. Ramrin L., Kanitpun R., Kulorn S., Srikaewkheaw N. and Ekgatat M Preliminary evaluation of fluorecence polarization aay for diagnoi of brucelloi in Thailand. The 5th Thailand-Japan Joint Conference on Animal Health. National Intitute of Animal Health, Bangkok, Thailand, June 6-8, (Poter preentation). 5. Kanitpun R., Chetiyawan P., Tongpun P., Choe-ngern W., Choomchuen P., Wongphol S., Ninprom T., Chaiyaert S., Sangkaew N., Kaewkalong S., Opachaitat P. and Ekgatat M. Trend of brucelloi in dairy cattle, goat and heep during 2012 to 2016 in Thailand. The 5th Thailand-Japan Joint Conference on Animal Health. National Intitute of Animal Health, Bangkok, Thailand, June 6-8, (Poter preentation). 6. Kaewkalong S., Chamchoy T., Srikaewkheaw N., Rakajit S., Kanitpun R. and Ekgatat M Seroprevalence of brucelloi in goat, heep and dairy cow in Regional Livetock 1 in Thailand, The 5th Thailand-Japan Joint Conference on Animal Health. National Intitute of Animal Health, Bangkok, Thailand, June 6-8, (Poter preentation). c) National conference: 8 OIE Reference Laboratory Report Activitie,

8 1. Cae tudy meeting: Public health and animal health The epidemiology tudy of human brucelloi in Suphanburi province (March 10, 2017) 2. Brucelloi diagnoi and inter-laboratory proficiency teting, and tuberculoi diagnoi and in animal, NIAH, Bangkok (March 14, 2017) 3. Brucelloi and tuberculoi diagnoi and, Region 8, Suratthani province (April 25, 2017) 4. Sero-diagnoi in animal dieae and problem, NIAH and Regional Veterinary Reearch and Development Center, NIAH, Bangkok (May 30-31, June 1, 2017) 5. Conference on brucelloi tandard diagnoi in Thailand, Veterinarian and takeholder, Amari Don Muang Airport Hotel, Bangkok (June 13, 2017) 6. Brucelloi diagnoi in Goat, Veterinary Reearch and Center, Wetern region, Ratchaburi province, (Augut 23, 2017) 7. Dairy Herd Health Conference: Brucelloi and tuberculoi diagnoi and, Pak Chong, Nakhon Ratchaima province (Augut 29, 2017) 8. ILPT for bovine brucelloi, Diviion of Veterinary Inpection and Quarantine, DLD, Pathumthani province (vember 6, 2017) d) Other: (Provide webite addre or link to appropriate information) 4 1. Field Epidemiology Training Program for Veterinarian (FETPV): Introductory coure on epidemiology and biotatitic. June 9, 2017, and July 13-14, FETP meeting room. Minitry of Public Health. (Advior) 2. Field Epidemiology Training Program for Veterinarian (FETPV): Animal Outbreak Invetigation Preentation: Rik factor for brucelloi poitivity of VAEPS in Inner Mongolia, China. vember 1, Department of Livetock Development. (Commentator) 3. FETPV Conference on Animal Outbreak Invetigation (Reearch Preentation), vember 8-10, 2017 (Advior) 4. The Fifth Conference on Animal Health Situation/Surveillance Data Analyi, 2017 Under FETPV, vember 13-15, 2017, votel Bangkok Platinum Hotel, Pratunam, Bangkok. (Advior and commentator) ToR 7: To provide cientific and technical training for peronnel from OIE Member Countrie To recommend the precribed and alternative tet or vaccine a OIE Standard 14. Did your laboratory provide cientific and technical training to laboratory peronnel from other OIE Member Countrie? a) Technical viit: 9 b) Seminar: 0 c) Hand-on training coure: 0 d) Internhip (>1 month): 0 8 OIE Reference Laboratory Report Activitie, 2017

9 Type of technical training provided (a, b, c or d) Country of origin of the expert() provided with training. participant from the correponding country a Japan 1 a Bhutan 1 a Taiwan 2 a Autralia 2 a Lao 2 a Autralia 1 a Indoneia (1), Myanmar (1), Vietnam (1), Cambodia (2), Philippine (1) (FETPV coure) 6 a Autralia 1 a APQA, Republic of Korea 9 ToR 8: To maintain a ytem of quality aurance, bioafety and bioecurity relevant for the pathogen and the dieae concerned 15. Doe your laboratory have a Quality Management Sytem certified according to an International Standard? Quality management ytem adopted ISO 17025:2005 ISO 9001:2008 Certificate can (PDF, JPG, PNG format) Certificate of ISO pdf Certificate of ISO 9001.jpg 16. I your laboratory accredited by an international accreditation body? Tet for which your laboratory i accredited Accreditation body RBT CFT ILAC-MRA by Bureau of Laboratory Quality Standard, Department of Medical Science ILAC-MRA by Bureau of Laboratory Quality Standard, Department of Medical Science OIE Reference Laboratory Report Activitie,

10 17. Doe your laboratory maintain a biorik management ytem for the pathogen and the dieae concerned? (See Manual of Tet and Vaccine for Terretrial Animal, Chapter 1.1.4) ToR 9: To organie and participate in cientific meeting on behalf of the OIE 18. Did your laboratory organie cientific meeting on behalf of the OIE? 19. Did your laboratory participate in cientific meeting on behalf of the OIE? ToR 10: To etablih and maintain a network with other OIE Reference Laboratorie deignated for the ame pathogen or dieae and organie regular inter-laboratory proficiency teting to enure comparability of reult 20. Did your laboratory exchange information with other OIE Reference Laboratorie deignated for the ame pathogen or dieae? 21. Wa your laboratory involved in maintaining a network with OIE Reference Laboratorie deignated for the ame pathogen or dieae by organiing or participating in proficiency tet? Purpoe of the proficiency tet: 1 Role of your Reference Laboratory (organier/ participant). participant Participating OIE Ref. Lab/ organiing OIE Ref. Lab. Aia- Bovine Brucelloi ILPT 2016 Final report preparation organizer 37 laboratorie Adviory and approval by Dr. Bruno Garin-Batuji, ANSES 1 validation of a diagnotic protocol: pecify the tet; quality of vaccine: pecify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratorie for the ame dieae on cientific reearch project for the diagnoi or of the pathogen of interet? 10 OIE Reference Laboratory Report Activitie, 2017

11 ToR 11: To organie inter-laboratory proficiency teting with laboratorie other than OIE Reference Laboratorie for the ame pathogen and dieae to enure equivalence of reult 23. Did your laboratory organie or participate in inter-laboratory proficiency tet with laboratorie other than OIE Reference Laboratorie for the ame dieae? te: See Interlaboratory tet comparion in: Laboratory Proficiency Teting at: ee point 1.3 ToR 12: To place expert conultant at the dipoal of the OIE 24. Did your laboratory place expert conultant at the dipoal of the OIE? 25. Additional comment regarding your report: OIE Reference Laboratory Report Activitie,

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