OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2017 This report has been submitted : :49:43 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Contagious bovine pleuropneumonia Address of laboratory: Istituto Zooprofilattico Sperimentale dell'abruzzo e Molise, Via Campo Boario Teramo ITALY Tel.: Fax: address: m.scacchia@izs.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Prof Mauro Mattioli, DVM, General Director Name (including Title and Position) of OIE Reference Expert: Massimo Scacchia, DVM, Head of Animal Health Laboratory Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/No) Total number of test performed last year Indirect diagnostic tests Nationally Internationally c-elisa YES 0 13 CFT YES Immuno-blotting YES 0 3 Direct diagnostic tests Nationally Internationally Mmm Isolation YES PCR YES RT-PCR YES PCR-RFLP YES IMMUNOHISTOCHEMISTRY YES MICROARRAY YES 0 3 NGS YES 0 47 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? NOTE: Currently, there are 22 laboratories that produce Standard Reference Reagents officially recognised by the OIE for 19 2 OIE Reference Laboratory Reports Activities, 2017
3 diseases/pathogens. Please click the following link to the list of OIE-approved International Standard Sera: If the reagent is not listed on this page, it is NOT considered OIE-approved. The next two questions allow you to indicate non-oie-approved diagnostic reagents. Disease Test Available from Contagious bovine pleuropneumonia Dr Massimo Scacchia CESME, Istituto Zooprofilattico Both irradiated and non-irradiated sera are available. Sperimentale dell Abruzzo e del Please contact the Reference Laboratory (Dr Pini) for Molise 'G. Caporale', Via Campo advice on their suitability for different types of Boario, serological test (complement fixation test, indirect or Teramo, Italy competitive enzyme-linked immunosorbent assay) Tel: ( ) Fax: ( ) m.scacchia@izs.it Type of reagent available Related diagnostic test Produced/ Supply imported Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Name of recipient OIE Member Countries Positive Serum CFT Produced <10mL mL mL >500mL <10mL mL mL >500mL TURKEY Negative Serum CFT Produced <10mL mL mL >500mL <10mL mL mL >500mL TURKEY 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients Antigen CFT Produced 350 ml 3 ml 30 ml 4 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? No OIE Reference Laboratory Reports Activities,
4 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? No 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month) No. samples received for provision of diagnostic support No. samples received for provision of confirmatory diagnoses NIGERIA May TANZANIA March 0 1 ZAMBIA June Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? 4 OIE Reference Laboratory Reports Activities, 2017
5 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided ZAMBIA CBPP Post mortem examination, gross lesions description and scoring of its. Training on the job, Central Veterinary Research Institute (CVRI), Lusaka, Zambia TURKEY Indirect and Direct CBPP diagnostic tests. Disease control strategy. Training on the job, CBPP OIE Reference Laboratory c/o IZSAM, Teramo, Italy KENYA CBPP Post mortem examination, gross lesions description and scoring of its. Training on the job, KALRO, Nairobi, Kenya TURKEY Mandate of the CBPP OIE Reference Laboratory Classroom activity, PVCI Pendik, Turkey ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,
6 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Pathogenic studies of Mmm infection 3 years Study of first stage of inflammatory reactions using nasal mucosae, trachea and lung explants. CVRI-Lusaka, Zambia, KALRO-Nairobi, Veterinary School-Teramo, Italy ZAMBIA Antimicrobial an vaccine efficacy studies 1 year Collaboration on evaluation of CBPP pathology in antimicrobial and vaccine efficacy studies CVRI-Lusaka, Zambia, KALRO-Nairobi, Kenya, GALVmed-Edinburgh, Scotland, Veterinary School-Teramo, Italy KENYA Studies on: Pathogenesis of Mmm 3 years Role of host immune response in the pathogenesis of Contagious Bovine Pleuropneumonia using immunohistochemistry and confocal laser scanning microscopy. CVRI-Lusaka, Veterinary School-Teramo ZAMBIA Pathogenic studies of Mmm 3 years Study of interaction between Mmm and host cells using OMIC approach CVRI-Lusaka, Biotechnology Faculty Teramo, Veterinary School-Teramo, Italy ZAMBIA CBPP gross lesions; new score system 2 years CBPP gross lesions; Produce and develop a new scoring system CVRI-Lusaka, ILRI-Nairobi, GALVmed-Edinburgh, Veterinary School-Teramo ZAMBIA ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 3 1. Di Teodoro G., Marruchella G., Di Provvido A., Orsini G., Ronchi G.F., D Angelo A.R., D Alterio N., Sacchini F., Scacchia M. Respiratory explants as a model to investigate early events of contagious bovine pleuropneumonia infection. Veterinary Research, accepted for publication. 2. Provvido A., Di Teodoro G., Muuka G., Marruchella G., Scacchia M. "Lung lesion score system in cattle: proposal 6 OIE Reference Laboratory Reports Activities, 2017
7 for Contagious bovine pleuropneumonia. Tropical Animal Health and Production 2017 Sep 27. doi: /s , in press. 3. Luciani M., Armillotta G., Manna L., Ciarelli A., Di Febo T., Krasteva I., Pini A., Sacchini F., D Angelo A.R. "Selection of a monoclonal antibody by ELISA, immunoblotting and Quartz Crystal Microbalance technology for immunohistochemical detection of Mycoplasma mycoides subsp. mycoides". Veterinaria Italiana, in press. b) International conferences: 3 1. Giovanni Di Teodoro, Giuseppe Marruchella, Andrea Di Provvido, Gianluca Orsini, Gaetano Federico Ronchi, Anna Rita D Angelo, Massimo Scacchia. Respiratory explants as a model to investigate early onset of contagious bovine pleuropneumonia infection. Annual meeting of American College of Veterinary Pathologists, November 2017, Vancouver (British Columbia). 2. Giovanni Di Teodoro, Francesco Mosca, Giuseppe Marruchella, Andrea Di Provvido, Flavio Sacchini, Pietro Giorgio Tiscar, Massimo Scacchia Polymorphonuclear cells and reactive oxygen species in Contagious bovine pleuropneumonia: new insight from in vitro investigations. Annual meeting of American College of Veterinary Pathologists, November 2017, Vancouver (British Columbia). 3. Giovanni Di Teodoro, Giuseppe Marruchella, Anna Rita D'Angelo, Andrea Di Provvido, Geoffrey Muuka, Massimo Scacchia Renal involvement in Contagious bovine pleuropneumonia: pathological and immunohistochemical findings. Annual meeting of American College of Veterinary Pathologists, November 2017, Vancouver (British Columbia). c) National conferences: 0 d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 2 b) Seminars: 1 c) Hands-on training courses: 3 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country a) Ethiopia 15 a) Mozambique 15 b) Turkey 45 c) Turkey 3 c) Zambia 5 c) Kenya 10 OIE Reference Laboratory Reports Activities,
8 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) IZSAM and ACCREDIA.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited CFT Immuno-blotting PCR-RFLP Accreditation body ILAC Accredia ILAC Accredia ILAC Accredia 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 8 OIE Reference Laboratory Reports Activities, 2017
9 National/ International Title of event Co-organiser Date (mm/yy) Location No. Participants International IZSAM in Africa: the strengthening of research capacity through the consolidation of a collaborative network no March 2017 Teramo, Italy 50 International Final International Workshop of CBPP Twinning Project PVCI Pendik, Turkey July 2017 PVCI Pendik, Turkey 45 International Strengthening ERFAN through the support of OIE Reference Laboratories/Collaborating Centres in the SADC Region University of Pretoria, South Africa October 2017 University of Pretoria, South Africa Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented IZSAM in Africa: the strengthening of research capacity through the consolidation of a collaborative network March 2017 IZSAM, Teramo, Italy Speaker Research and Development in Africa: building a lasting and trustful relations Central Veterinary Laboratory Maputo; Internal seminar May 2017 CVL, Maputo, Mozambique Speaker IZSAM role and activities in African countries Final International Workshop CBPP Twinning Project July 2017 PVCI Pendik, Turkey Speaker The mandate of the CBPP OIE Reference Laboratory REseau MEditerranéen de Santé Animale REMESA, July 2017 Naples, Italy Speaker ERFAN; Enhancing Research For Africa Network Second Research Coordination Meeting, IAEA August 2017 IAEA, Vienna, Austria Speaker Quality system: an important part of a long-term scientific collaboration with African countries National Animal Heath Diagnostic and Investigation Center; Internal seminar August 2017 NAHDIC, Sebeta, Ethiopia Speaker The CBPP OIE Reference Laboratory research Activities OIE Reference Laboratory Reports Activities,
10 ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant) No. participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. CFT Participant 8 Molecular Diagnosis Participant 4 CBPP OIE Ref Lab c/o Botswana National Veterinary Laboratory CBPP OIE Ref Lab c/o Botswana National Veterinary Laboratory 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories ERFAN-SADC Region CBPP Working group CBPP; Disease control and standardization of diagnostic methods in SADC Region CBPP OIE Reference Laboratory c/o Botswana National Veterinary Laboratory ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? 10 OIE Reference Laboratory Reports Activities, 2017
11 Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1 No. participating laboratories Region(s) of participating OIE Member Countries CF Test 8 Africa Americas Asia and Pacific Europe Middle East Molecular Diagnosis; PCR 4 Africa Americas Asia and Pacific Europe Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) CBPP OIE ad hoc Group OIE Headquarter, Paris, France Group of CBPP experts 25. Additional comments regarding your report: OIE Twinning Project on CBPP: - A project between IZSAM, as Parental Laboratory and Pendik Veterinary Control Institute (PVCI), Turkey, as Candidate Laboratory, started in 2015 was concluded, successfully, in July The Candidate Laboratory organised the Final International Workshop with more than forty-fives foreign and Turkish participants; - The National Animal Health Diagnostic Centre of Sebeta, Ethiopia contacted the OIE Reference Laboratory in order to present an OIE Twinning Project proposal for The OIE Reference Laboratory: Was involved in research projects on CBPP sponsored by GALVmed; Created and organised the Enhancing Research for Africa Network (ERFAN). It main task is to allow the Parental Laboratory to continue supporting the Candidate Laboratory once the OIE Twinning Project is concluded. The aim is to tackle the sustainability of the Regional Pole of Excellence, created after an OIE Twinning Project; Sequenced the whole genome of 44 Mmm strains, using NGS method, coming from Nigeria (17), Tanzania (1), Zambia (4) and historical strains coming from Botswana (3), Portugal (1), Italy (15) and standard strains (3); Provided also technical support and expertise to the International Livestock Research Institute of Nairobi (Kenya) to carry out research activities sponsored by GALVmed, to develop a live vaccine for Contagious Caprine Pleuropneumonia. OIE Reference Laboratory Reports Activities,
12 12 OIE Reference Laboratory Reports Activities, 2017
OIE Reference Laboratory Reports Activities
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