OIE Collaborating Centres Reports Activities

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1 OIE Collaborating Centres Reports Activities Activities in 2016 This report has been submitted : :33:18 Title of collaborating centre: Food-Borne Zoonotic Parasites Address of Collaborating Centre: Canadian Food Inspection Agency 116 Veterinary Road Saskatoon Saskatchewan S7N 2R3 CANADA Tel.: Fax: address: Website: Name of Director of Institute (Responsible Official): Marc Sabourin Name (including Title and Position) of Head of the Collaborating Centre (formally OIE Contact Point): Dr. Brad Scandrett Acting Section Head, Centre for Food-borne and Animal Parasitology Name of writer: Brad Scandrett OIE Collaborating Centres Reports Activities,

2 ToR: To provide services to the OIE, in particular within the region, in the designated specialty, in support of the implementation of OIE policies and, where required, seek for collaboration with OIE Reference Laboratories ToR: To identify and maintain existing expertise, in particular within its region 1. Activities as a centre of research, expertise, standardisation and dissemination of techniques within the remit of the mandate given by the OIE Disease control Provision of diagnostic services and scientific advice to CFIA Policies and Programs, and Operations, Branches Domestic disease investigations of Taenia saginata/cysticercus bovis infections in cattle, and human cyclosporosis and giardiosis infections Epidemiology, surveillance, risk assessment, modelling Surveillance for food-borne parasites in animals, animal products and fresh produce for domestic food safety, disease control, and import/export purposes National surveillance program for detection of Trichinella in domestic swine via digestion assay; Research survey for detection of Trichinella spp. in omnivorous and carnivorous wildlife in proximity to domestic swine in Canada via digestion assay; National surveillance program for detection of Cyclospora, Cryptosporidium, Toxoplasma, and Giardia contamination of fresh domestic and imported produce via qpcr-mca and npcr; Research survey for detection of Toxoplasma in domestic swine via meat juice serology Participation on CFIA assessment team evaluating foreign country food safety controls for food-borne parasite contamination of exported produce On-site provision of scientific advice to CFIA assessment team evaluating foreign country food safety controls to mitigate Cyclospora contamination of fresh fruit and vegetables for export Training, capacity building Training and certification of CFIA and industry analysts to perform diagnostic assays for the detection of food-borne parasites Zoonotic food-borne parasites from domestic and imported food sources Ad hoc training to graduate students performing research studies involving food-borne zoonotic parasites Trichinella spp. and Toxoplasma Internal capacity building to develop expertise in whole genome/next generation sequencing This initiative is currently focussed on Toxoplasma Diagnosis, biotechnology and laboratory Routine diagnosis and surveillance of food-borne parasites for domestic disease control, food safety, and import/export purposes Detection and diagnosis of various food-borne parasites by direct (microscopic examination, digestion assay, PCR) and indirect (serological) methods, including zoonotic coccidia, Giardia, Taenia, and Trichinella 2 OIE Collaborating Centres Reports Activities, 2016

3 Development, optimization, standardization, and/or validation of methods to detect food-borne zoonotic parasites Optimization of an in-house indirect ELISA to detect Trichinella in domestic swine; Development of a celisa to detect Trichinella in domestic swine and other host species; Development and/or validation of in-house and commercial serological methods (IFA, MAT, ELISA) to detect Toxoplasma in meat juice samples from domestic food animals and wildlife; Assessment of a commercial digestion assay kit for the detection of Trichinella in pigs, horses, and wildlife; Optimization of LAMP assays for the detection of Toxoplasma and Giardia contamination of fresh produce ToR : To propose or develop methods and procedures that facilitate harmonisation of international standards and guidelines applicable to the designated specialty 2. Proposal or development of any procedure that will facilitate harmonisation of international regulations applicable to the surveillance and, food safety or animal welfare Proposal title /Content Applicable area Optimization of LAMP assays for the detection of Toxoplasma and Giardia Molecular detection of food-borne protozoal parasites Optimization of in-house indirect ELISA using E/S antigen for the detection of Trichinella in pigs Trichinella spiralis in domestic swine Development of a celisa for the detection of Trichinella in pigs (and other host species) Trichinella spp in domestic swine and other host species Development and/or validation of serological methods (MAT, ELISA, IFA) for the detection of Toxoplasma in food animals and wildlife Detection of Toxoplasma gondii in blood, serum or meat juice samples from various food animals and wildlife Evaluation of the performance of a commercial digestion assay kit for the detection of Trichinella in pork, horse meat, and wildlife samples Detection of Trichinella spp. in pork, horse meat, and bear and wolf samples ToR: To establish and maintain a network with other OIE Collaborating Centres designated for the same specialty, and should the need arise, with Collaborating OIE Collaborating Centres Reports Activities,

4 Centres in other disciplines ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other centres, laboratories or organisations 3. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres (CC), Reference Laboratories (RL), or organisations designated for the same specialty, to coordinate scientific and technical studies? Yes Name of OIE CC/RL/other organisation(s) Location Region of networking Centre Purpose OIE Collaborating Centre for Food-borne Zoonotic Parasites from the European Region Maisons-Alfort, France Africa Americas Asia and Pacific Europe Middle East Exchange of scientific advice and proficiency samples; collaboration on scientific publications OIE Reference Lab for Trichinellosis Rome, Italy Africa Americas Asia and Pacific Europe Middle East Exchange of scientific advice via shared roles as OIE Reference Labs for Trichinellosis and as Executive Committee members of the International Commission on Trichinellosis (ICT) 4. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres, Reference laboratories, or organisations in other disciplines, to coordinate scientific and technical studies? Yes Name of OIE CC/RL/other organisation(s) Location Region of networking Centre Purpose OIE Collaborating Centre for Research, Diagnosis and Surveillance of Wildlife Pathogens (Canadian Wildlife Health Cooperative/CWHC) Canada Africa Americas Asia and Pacific Europe Middle East Procurement of wildlife samples and data for Trichinella surveillance project ToR: To place expert consultants at the disposal of the OIE. 5. Did your Collaborating Centre place expert consultants at the disposal of the OIE? 4 OIE Collaborating Centres Reports Activities, 2016

5 No ToR: To provide, within the designated specialty, scientific and technical training to personnel from OIE Member Countries 6. Did your Collaborating Centre provide scientific and technical training, within the remit of the mandate given by the OIE, to personnel from OIE Member Countries? Yes a) Technical visits: 1 b) Seminars: 1 c) Hands-on training courses: 1 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Content Country of origin of the expert(s) provided with training No. participants from the corresponding country a, b, c Molecular and serological methods for Trichinella and Toxoplasma India 1 ToR: To organise and participate in scientific meetings and other activities on behalf of the OIE 7. Did your Collaborating Centre organise or participate in the organisation of scientific meetings on behalf of the OIE? No ToR: To collect, process, analyse, publish and disseminate data and information relevant to the designated specialty 8. Publication and dissemination of any information within the remit of the mandate given by the OIE that may be useful to Member Countries of the OIE a) Articles published in peer-reviewed journals: 4 Lalonde, L.F. and Gajadhar, A.A Optimization and validation of methods for isolation and real-time PCR identification of protozoan oocysts on leafy green vegetables and berry fruits. Food and Waterborne Parasitology, 2:1-7. Lalonde, L.F. and Gajadhar, A.A Detection of Cyclospora cayetanensis, Cryptosporidium spp. and Toxoplasma gondii on imported leafy green vegetables in Canadian survey. Food and Waterborne Parasitology, 2: Al-Adhami, B.H., Simard, M., Hernandez-Ortiz, A., Boireau, C., Gajadhar, A.A Development and evaluation of a modified agglutination test for diagnosis of Toxoplasma infection using tachyzoites cultivated in cell culture. Food and Waterborne Parasitology, 2: OIE Collaborating Centres Reports Activities,

6 Konecsni, K., Scheller, C., Scandrett, B., Buholzer, P., Gajadhar, A. Evaluation of the PrioCHECK Trichinella AAD Kit for the digestion and recovery of larvae in pork, horsemeat and wildlife. Manuscript submitted for publication October b) International conferences: 0 c) National conferences: 1 Lobanov, V.A Research activities related to the diagnosis and control of equine piroplasmosis and Trichinella infection. Oral presentation at the CFIA Animal Health Research Forum; Ottawa, Canada, February 16-17, d) Other (Provide website address or link to appropriate information): 1 Al-Adhami, B.H An overview of diagnostic and research activities on equine piroplasmosis and toxoplasmosis at CFAP. CFIA Animal Health Science Webinar Series, November 23, OIE Collaborating Centres Reports Activities, 2016

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