OIE Reference Laboratory Reports Activities

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1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :20:17 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis (Brucella abortus) Address of laboratory: 177 Hyeoksin 8-ro Gimcheon-si, Gyeongsangbukdo KOREA (REP. OF) Tel.: Fax: address: herm@korea.kr Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Bong-kyun, DVM, MS, PhD QIA Commissioner Name (including Title and Position) of OIE Reference Expert: Dr. Moon Her, DVM, MS, PhD Senior Researcher Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally RBT SAT ELISA FPA Dip-stick Direct diagnostic tests Nationally Internationally PCR MLVA Bacterial culture ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016

3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients RBT antigen RBT Produced 33ml Africa Americas Asia and Pacific Europe Middle East SAT antigen SAT Produced 5595ml Africa Americas Asia and Pacific Europe Middle East CF antigen CFT Produced 100ml Africa Americas Asia and Pacific Europe Middle East Multiplex PCR kit PCR Produced 1,500 tests 192 tests 1 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? OIE Reference Laboratory Reports Activities,

4 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses THAILAND 4 89 THAILAND Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided THAILAND Brucella isolation and biochemical tests In loco THAILAND PCR(multiplex) for Brucella In loco THAILAND Serological tests (RSAT for B. canis) In loco ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 4 OIE Reference Laboratory Reports Activities, 2016

5 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country The molecular epidemiological analysis of Brucella species from a variety of animals and application on diagnostics for Brucella canis 3 years( ) Identifying Brucellosis in Thailand and epidemiological correlation between Brucella isolates between two countries and application and evaluation on serologic diagnostics of B. canis National Institute for Animal Health THAILAND ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 4 1. Kim JY, Kang SI, Lee JJ, Lee K, Sung SR, Erdenebaataar J, Vanaabaatar B, Jung SC, Park YH, Yoo HS, Her M. (2016). Differential diagnosis of Brucella abortus by real-time PCR based on a single-nucleotide polymorphisms J. Vet. Med. Sci. 78(4): Lee JJ, Simborio HL, Reyes AW, Hop HT, Arayan LT, Lee HJ, Min W, Her M, Rhee MH, Watarai M, Chang HH, Kim S (2016). Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus. BMC Microbiology. 16 : Kim JY, Kang SI, Erdenebaataar J, Vanaabaatar B, Ulziisaikhan G, Khursbaatar O, Lee K, Lee JJ, Sung SR, Jung SC, Park YH, Yoo HS, Her M (2016). Distinctive phenotypic and molecular characteristics of Brucella abortus strains isolated from Mongolia. Turk J Vet Anim Sci. 40: Quang Lam Truong, Kiju Kim, Jong-Taek Kim, Moon Her, Suk-Chan Jung, Tae-Wook Hahn (2016). Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea. Kor. J. Vet. Res. 56(3) : b) International conferences: 4 1. Jin Ju Lee, So-Ra Sung, Eun Ji Yum, Se Chul Kim, Sung Jin Seo, Soon-Seek Yoon, Ji-Youl Jung, Suk Chan Jung, Moon Her (2016). Screening of immune responses in canine brucellosis; Approach related to host-pathogen interaction, (2016 Cell Symposia) OIE Reference Laboratory Reports Activities,

6 2. Jin Ju Lee, Ji-Yeon Kim, So-Ra Sung, Eun Ji Yum, Se Chul Kim, Sung Jin Seo, Suk Chan Jung, Moon Her (2016). Evaluation of storage stability in LPS antigen for indirect milk-elisa screening dairy brucellosis. (69th Brucellosis Research Conference). 3. Eun Ji Yum, Jin Ju Lee, So-Ra Sung, Se Chul Kim, Sung Jin Seo, Suk Chan Jung, Moon Her (2016). Comparison of serological tests based on microbiological culture for the diagnosis of canine brucellosis. (69th Brucellosis Research Conference). 4. Se-Chul Kim, Jin-Ju Lee, So-Ra Sung, Eun Ji Yum, Sung Jin Seo, Suk Chan Jung, Moon Her (2016). Specific identification of the genus Brucella using SNP of bcsp 31. (69th Conference of Research Workers in Animal Diseases). c) National conferences: 5 1. Jin Ju Lee, So-Ra Sung, Eun Ji Yum, Se Chul Kim, Sung Jin Seo, Soon-Seek Yoon, Ji-Youl Jung, Suk Chan Jung, Moon Her (2016). Different immune responses induced by B. canis respecting to host-pathogen interaction (Autumn meeting, Korean Society of Veterinary Science). 2. Eun Ji Yum, Jin Ju Lee, So-Ra Sung, Se Chul Kim, Sung Jin Seo, Suk Chan Jung, Moon Her (2016). Comparison of the accuracy of serological tests with microbial culture for the diagnosis of canine brucellosis. (Autumn meeting, Korean Society of Veterinary Science). 3. Se-Chul Kim, Jin-Ju Lee, So-Ra Sung, Eun Ji Yum, Jin Sung Seo, Suk Chan Jung, Moon Her (2016). Specific identification of Brucella abortus using coordination of SNPs (Autumn meeting, Korean Society of Veterinary Science). 4. Jin Ju Lee, So-Ra Sung, Eun Ji Yum, Se Chul Kim, Sung Jin Seo, Soon-Seek Yoon, Ji-Youl Jung, Suk Chan Jung, Moon Her (2016). Evaluation of Brucella canis isolation methods from whole blood in dogs for the diagnosis of brucellosis (Autumn meeting, Korean Society of Preventive Veterinary Medicine). 5. So-Ra Sung, Moon Her, Ji-Yeon Kim, Sung-Il Kang, Seon Hwa Kim, Hee Soo Lee, Jin Ju Lee (2016). Prevalence of Brucellosis in dairy cattle in South Korea with focus on epidemiology, hazard factors and control opportunities, Korean Society of Preventive Veterinary Medicine). d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 9 b) Seminars: 7 c) Hands-on training courses: 7 d) Internships (>1 month): 0 6 OIE Reference Laboratory Reports Activities, 2016

7 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Thailand 2 a, b, c Fiji 2 a, b, c Bangladesh 2 a, b, c Sri Lanka 3 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO/IEC Certificate scan (PDF, JPG, PNG format) KOLAS ISOIEC pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited MRT RBT SAT ELISA FPA PCR MLVA Bacterial culture Accreditation body 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) OIE Reference Laboratory Reports Activities,

8 ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented 2016 Cell Symposia 10/16 United States 1 presenting poster Ref 13. b) International conferences 69th International Brucellosis research conference 11/16 India 3 presenting posters Ref 13. b) International conferences ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. EU Brucellosis Bovine Serum Proficiency Ring Trial 2016 Brucellosis Ring Trial for RBT and ielisa 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? 8 OIE Reference Laboratory Reports Activities, 2016

9 Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories The molecular epidemiological analysis of Brucella species from a variety of animals and application on diagnostics for Brucella canis Identifying Brucellosis in Thailand and epidemiological correlation between Brucella isolates between two countries and application and evaluation on serologic diagnostics of B. canis Immuno-serology Lab. of National Institute for Animal Health in Thailand ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,

OIE Reference Laboratory Reports Activities

OIE Reference Laboratory Reports Activities Activities in 2016 This report h been submitted : 2017-01-11 18:55:37 Name of disee (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis