Methods development to detect antibiotic activity in water samples
|
|
- Peregrine Walton
- 5 years ago
- Views:
Transcription
1 Methods development to detect antibiotic activity in water samples Stefan Kools (Grontmij AquaSense) Marta Wilgosz (Grontmij AquaSense, WUR) Evertjan van de Brandhof (RIVM) Gerard Stroomberg (Waterdienst) Mariel Pikkemaat (RIKILT) 1
2 Who is who? Grontmij AquaSense Consultancy and ecotoxicology laboratories Waterdienst Directorate-General for Public Works and Water Management (Rijkswaterstaat) RIVM National Institute for Public Health and the Environment RIKILT WUR Institute of Food Safety Wageningen University and Research 2
3 Presentation Introduction to study on emerging substances Method development Application (examples) Method optimization and outlook 3
4 Background NORMAN has identified a list of the currently most frequently discussed emerging substances and emerging pollutants today. Examples of this list are surfactants, pharmaceuticals and personal care products, methyl tert-butyl ether (MTBE) and other related petrol additives and their degradation products, polar pesticides and their degradation products and various proven or suspected endocrine disrupting compounds (EDCs). Another example is nanoparticles, which behave aerodynamically like gas molecules and have a large surface area per unit mass. 4
5 Background NORMAN has identified a list of the currently most frequently discussed emerging substances and emerging pollutants today. Examples of this list are surfactants, pharmaceuticals and personal care products, methyl tert-butyl ether (MTBE) and other related petrol additives and their degradation products, polar pesticides and their degradation products and various proven or suspected endocrine disrupting compounds (EDCs). Another example is nanoparticles, which behave aerodynamically like gas molecules and have a large surface area per unit mass. 5
6 Pharmaceuticals antibiotics Oroginally natural substances against bacteria, fungi, protozoa: Bactericidal kill microbes Bacteriostatic inhibit grotwh st antibiotic penicillin Worldwide use: antibiotic era Treatment in human and veterinary medicine Threat: antibiotic resistance prudent use In veterinary medicine for prevention and as growth promoters (2004 stop in EU) 6
7 Antibiotics fate and distribution WWTP
8 Sold antibiotics (t) 1200 Relation between antibiotics and meat production 1000 UK France 800 Spain Netherlands Italy Germany 200 Others Belgium+Lux. Denmark Kools et al., 2008, IEAM Meat production (x1000 t) carcasse weight
9 Use in households (NL) Older population uses more medicines Expected life >65 yr: +60% 9
10 Apples and oranges? Antibiotics groups Aminoglycosides (A) Macrolides (M) Beta lactams (B) Quinolones (Q) Sulfonamides (S) Tetracyclines (T) 10
11 Bioassay measurement Chemical analysis: accurate identification? metabolites? Bioassays Potential identification of activity Cf. Steroids (Estrogens, Androgens, etc). Cf. Dioxines Including active metabolites 11
12 Methods Developed at RIKILT (Food Safety) The Nouws antibiotic test (NAT) slaughter animals (residue analysis) 5 test plates group-specific identification T-plate; Q-plate; M&B-plate; A-plate and S-plate PRINCIPLE (Specific) BACTERIAL GROWTH INHIBITION IN PRESENCE OF ANTIBIOTICS 12
13 Water Scan Test Antibiotic present growth inhibition 2 16 mm linear correlation between inhibition zone and concentration 13
14 The tests Different agar compositions were used with different bacteria strains, antibiotic supplements to prepare them Applying samples on plates incubate at 30 C (T, Q, M/B) 37 C (S,A) 1 concentration Dilution series Measurements after 24 h incubation Water Scan Test Inhibition zones were measured 96 well plate test Optical density (O.D.) (also at T 0 ) was measured 14
15 Method development Questions? Extraction method needed SPE, special resins (XAD)? Detection limit Sensitivity? Specificity? Cross-reactivity? 15
16 SPE extraction 16
17 XAD 4/8 extraction XAD 4/8 two synthetic resins used to extract substances 17
18 Spike (mix with log Kow range) log Kow Group Antibiotic CAS no Molecular formula Experimental Ecosar estim [kowwin] Aminoglycosides Neomycin B C23H46N6O Aminoglycosides Streptomycin C21 H39 N7 O Aminoglycosides Apramycin C21 H41 N5 O Aminoglycosides Kanamycin sulfate C18H36N4O Tetracyclines Oxytetracycline [SPE] C22H24N2O Cephalosporines Cefazolin C14 H13 N8 O4 S Aminoglycosides Gentamicin C21 H43N5 O Cephalosporines Cefalonium C20H18N4O5S Tetracyclines Doxycycline=vibramycin C22H24N2O Tetracyclines Tetracycline [SPE] C22H24N2O Cephalosporines Cefacetrile C13 H13 N3 O6 S Quinolones Marbofloxacin Penicillines Nafcillin C21 H21 N2 O5 S1 Na Aminoglycosides Spectinomycin C14 H24 N2 O Cephalosporines Cefapirin C17H17N3O6S Tetracyclines Chlortetracycline [SPE] C22H23ClN2O Cephalosporines Cefoperazone C25 H27 N9 O8 S Sulfonamides Sulfadiazine C10 H10 N4 O2 S Sulfonamides Sulfadoxine C12 H14 N4 O4 S Quinolones Ciprofloxacin [SPE] C17H18FN3O Quinolones Sarafloxacin C20H17F2N3O Macrolides Lincomycin C18 H34 N2 O6 S Cephalosporines Cefalexin C16H17N3O4S Sulfonamides Sulfamethoxazole [SPE] C10 H11 N3 O3 S Quinolones Enrofloxacin C19H22FN3O3 0.7 Sulfonamides Trimethoprim C14H18N4O Sulfonamides Sulfamethazine [SPE] C12 H14 N4 O2 S Sulfonamides Dapsone C12 H12 N2 O2 S Tetracyclines Chloramphenicol C11H12Cl2N2O Penicillines Amoxicillin C16H19N3O5S Macrolides Tylosin C46 H77 N1 O Sulfonamides Sulfadimethoxine [SPE] C12 H14 N4 O4 S Quinolones Danofloxacin C19H20FN3O Quinolones Difloxacin C21H19F2N3O Penicillines Ampicillin C16 H19 N3 O4 S Quinolones Oxolinic acid C13H11NO5 1.7 Macrolides Oleandomycin C35 H61 N1 O Penicillines Penicillin G C16 H18 N2 O4 S Macrolides Erythromycine C37 H67 N1 O Penicillines Oxacillin C19 H19 N3 O5 S Quinolones Flumequine C14H12FNO Sulfonamides Baquiloprim C17 H20 N Penicillines Cloxacillin C19 H18 CL1 N3 O5 S Penicillines Dicloxacillin C19 H17 CL2 N3 O5 S Macrolides Tilmicosin C46 H80 N2 O Macrolides Valnemulin C31H52N2O5S Log Kow clusters:? "-1" "-1" - "-2"? "-2" 1. Neomycin 2. Oxytetracycline-OTC 3. Chlortetracycline-CTC 4. Sulfadiazine 5. Sulfamethoxazole-SMX 6. Oxolinic acid 7. Erythromycine 8. Flumequine 18
19 Recovery (%) Recovery patterns (spiked water) Water-Scan test Mix 1 - recovery SPE vs. XAD SPE Recovery [%] XAD Recovery [%] ,7 272, ,5 85,4 68,2 68,2 98,4 89, T Q M/B S A Plate 19
20 Conclusions Some cross-reactivity noted At plate A : aminoglycosides Specific? Limited effects (<< cyto toxicity) of others RIVM data Overview of indicative detection limits for >35 compounds ( ug/l) Waterdienst data 20
21 Application, some examples Monitoring Screening locations Review of treatment techniques New treatments, do they remove? Prioritization of locations Identification of hot-spots 21
22 End-of-pipe? Antibiotics in river water Monitoring/screening Hot-spots? 22
23 Screening the Dutch Meuse river basin (Waterdienst)
24 Screening the Dutch Meuse river basin (Waterdienst)
25 growth inhibition (%) growth inhibition (%) Example: use of antibiotic bioassay STP standard sewage treatment plant STP + sand filter Response patterns similar in 96-wells test 2 - Steenwijk after WWTP STP (standard only) Chloramphenicol 3 - Steenwijk after WWTP & sand filter Chloramphenicol STP + sand filtration t plate M/B plate S plate 120 t plate M/B plate S plate concentration (µg/l) concentration (µg/l) 25
26 NOEC groeiremming (Cf; 50%-effect) Antibiotics screening: locations sulfonamiden macroliden/ß-lactam quinolonen aminoglycosiden tetracyclinen Utrecht 02 Vlaardingen 03 Nijmegen 04 Ootmarsum 05 Amersfoort 26
27 NOEC groeiremming (Cf; 50%-effect) verwaarloosbaar Antibiotics screening: assessment sulfonamiden macroliden/ß-lactam quinolonen aminoglycosiden tetracyclinen Utrecht 02 Vlaardingen 03 Nijmegen 04 Ootmarsum 05 Amersfoort 27
28 NOEC groeiremming (Cf; 50%-effect) verwaarloosbaar aandacht ernstig Antibiotics screening: assessment sulfonamiden macroliden/ß-lactam quinolonen aminoglycosiden tetracyclinen Utrecht 02 Vlaardingen 03 Nijmegen 04 Ootmarsum 05 Amersfoort 28
29 Conclusion Method optimization (with interested parties) Available for routine screening in combination with other bioassays in combination with chemical analysis (for interested parties) Outlook: Possible application in research on the emission of antibiotics from households, hospitals and careinstitutions Contact: 29
Current EU Antibiotic Maximum Residue Limits
R-Biopharm Rhône Current EU Antibiotic Maximum Residue Limits March 2011 R-Biopharm Rhône Current EU Antibiotic Limits 2 Contents Antibiotics in Food Aminoglycosides...4 Ansamycins...4 Beta-lactams...5
More informationRevolutionary Milk Analysis
Revolutionary Milk Analysis 2 Milk quality is a major concern Farmers, producers and laboratories have to meet European and international rules. They must perform even more stricter controls to ensure
More informationOne Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics
Featured Application: Multiclass Veterinary Antibiotics on Raptor C8 by LC- One Analysis, One Column, Less than 9 Minutes for Over 0 Multiclass Antibiotics Highly efficient peak separation and fast analysis
More informationPremi Test. Art. No. R3900. R-Biopharm AG. Fast Determination of antibiotic residues in less than 4 hours
Premi Test Art. No. R3900 Fast Determination of antibiotic residues in less than 4 hours Easy Simple handling and no extraordinary cost intensive equipment needed Sensitive Detects antibiotic residues
More informationValidation of bee4sensor for Honey
Conffidence Workshop: Work package 2b - Antibiotics Detection, analysis and control of veterinary pharmaceuticals in food and feed Validation of bee4sensor for Honey EuroResidue VII 16 May 2012 Hotel Zuiderduin,
More informationCountry Report: Malaysia
Country Report: Malaysia Akma Ngah Hamid Director Central Region Veterinary Laboratory (CRVL) Dpt. of Veterinary Service Introduction Antimicrobials are essential drugs and used in human and veterinary
More informationAMR Industry Alliance Antibiotic Discharge Targets
AMR Industry Alliance Antibiotic Discharge Targets List of Predicted No-Effect Concentrations (PNECs) The members of the AMR Industry Alliance have developed a unified approach to establishing discharge
More informationPerformance Information. Vet use only
Performance Information Vet use only Performance of plates read manually was measured in three sites. Each centre tested Enterobacteriaceae, streptococci, staphylococci and pseudomonas-like organisms.
More informationDeborah A. Cera - Division of Compliance Center for Veterinary Medicine, FDA
Deborah A. Cera - Division of Compliance Center for Veterinary Medicine, FDA 1 1 Databases RVIS Interagency Database TRIMS An Interactive Database Containing Information Obtained During FDA/State Inspections
More informationAntimicrobials & Resistance
Antimicrobials & Resistance History 1908, Paul Ehrlich - Arsenic compound Arsphenamine 1929, Alexander Fleming - Discovery of Penicillin 1935, Gerhard Domag - Discovery of the red dye Prontosil (sulfonamide)
More informationFramework for monitoring antibiotic content and antibiotic resistance in the Danube Delta - the EnviroAMR project -
Framework for monitoring antibiotic content and antibiotic resistance in the Danube Delta - the EnviroAMR project - Dr. Cristian COMAN Institute of Biological Research Cluj-Napoca November 18 th November
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Minerva House Unit 2 Stoney Gate Road Spondon Derbyshire DE21 7RY Contact: Mr Ian Brown Tel: +44 (0)1332 890384 Fax: +44 (0)1332 666040 E-Mail:
More informationAntibiotics & Resistance
What are antibiotics? Antibiotics & esistance Antibiotics are molecules that stop bacteria from growing or kill them Antibiotics, agents against life - either natural or synthetic chemicals - designed
More informationProject Summary. Impact of Feeding Neomycin on the Emergence of Antibiotic Resistance in E. coli O157:H7 and Commensal Organisms
Project Summary Impact of Feeding Neomycin on the Emergence of Antibiotic Resistance in E. coli O157:H7 and Commensal Organisms Principal Investigators: Mindy Brashears, Ph.D., Texas Tech University Guy
More informationScreening and Identification Methods for official control of Banned Antibiotics and Growth promoters in Feedingstuffs
Screening and Identification Methods for official control of Banned Antibiotics and Growth promoters in Feedingstuffs SIMBAG FEED Competitive and sustainable growth programme (GROWTH) project G6RD-CT-2000-00413
More informationSelective toxicity. Antimicrobial Drugs. Alexander Fleming 10/17/2016
Selective toxicity Antimicrobial Drugs Chapter 20 BIO 220 Drugs must work inside the host and harm the infective pathogens, but not the host Antibiotics are compounds produced by fungi or bacteria that
More informationAvoiding residues and an FDA Inspection
Avoiding residues and an FDA Inspection James D. McKean, DVM, JD Extension Veterinarian Associate Director, Iowa Pork Industry Center Iowa State University x2mckean@iastate.edu USDA FSIS Residue Testing
More informationAntibiotics: mode of action and mechanisms of resistance. Slides made by Special consultant Henrik Hasman Statens Serum Institut
Antibiotics: mode of action and mechanisms of resistance. Slides made by Special consultant Henrik Hasman Statens Serum Institut This presentation Definitions needed to discuss antimicrobial resistance
More informationUSE OF ANTIBIOTICS FOR ANIMALS IN VIETNAM. Nguyen Quoc An Dept. of Animal Health MARD
USE OF ANTIBIOTICS FOR ANIMALS IN VIETNAM Nguyen Quoc An Dept. of Animal Health MARD Summary of antibiotics utilisation for animals Total of product s Products of Vietnam Total Enterprise s Imported drugs
More informationMedically Important Antibiotics in Animal Agriculture
Medically Important Antibiotics in Animal Agriculture Craig Lewis, DVM MPH Office of the Director Center for Veterinary Medicine Farm Foundation Antimicrobial Stewardship Workshop Davis, California October,
More informationVeterinary Feed Directive
Veterinary Feed Directive Medically Important Antibiotics in Animal Agriculture Outline Questions to Be Addressed What changes are being made and why? What drugs are affected, which ones are not? What
More informationThe development of a new multiplex dipstick for the simultaneous detection of sulfonamides, (fluoro)quinolones, tylosin and chloramphenicol in honey
The development of a new multiplex dipstick for the simultaneous detection of sulfonamides, (fluoro)quinolones, tylosin and chloramphenicol in honey Stefan Weigel (RIKILT), Vincent Chabottaux (Unisensor)
More informationMixtures of veterinary medicinal compounds in manured soils
Workshop Pharmaceuticals in Soil, Sludge and Slurry Mixtures of veterinary medicinal compounds in manured soils Nadine Tauchnitz Daniela Gildemeister, Silvia Berkner Dessau-Roßlau, 18th June to 19th June
More informationAnalysis of Multiclass Veterinary Drugs in Baby Food by Ultra Fast Chromatography with High Performance Triple Quadrupole Mass Spectrometry
Analysis of Multiclass Veterinary Drugs in Baby Food by Ultra Fast Chromatography with High Performance Triple Quadrupole Mass Spectrometry Charles Yang, 1 Dipankar Ghosh, 1 Mary Blackburn, 1 Jamie Humphries
More informationThere are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility
ANTIMICROBIAL SUSCEPTIBILITY TESTING ON MILK SAMPLES Method and guidelines There are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility
More informationAMU/AMR Policy for animals in Korea Jaehong CHANG, DVM, MS
AMU/AMR Policy for animals in Korea Jaehong CHANG, DVM, MS Deputy Director of Animal Health Management Division, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea Contents Background Consequence
More informationPesticide and antibiotic pollution in the Mekong Delta
Pesticide and antibiotic pollution in the Mekong Delta Chau, N.D.G., Renaud, F. G., Sebesvari, Z., Rosendal, I., Amelung, W 6 th WISDOM seminar, 10 14 June, 2013 Bonn Slide 1 Vortrag > Autor > Dokumentname
More informationDrug Use on the Farm & Antibiotic Resistance in Raw, Stored, & Treated Manures
Drug Use on the Farm & Antibiotic Resistance in Raw, Stored, & Treated Manures Jason Oliver, PhD Cornell PRO-DAIRY Dairy Environmental Systems Dairy Practices Council Annual Conference Buffalo, NY Nov.
More informationAntibiotics. Antimicrobial Drugs. Alexander Fleming 10/18/2017
Antibiotics Antimicrobial Drugs Chapter 20 BIO 220 Antibiotics are compounds produced by fungi or bacteria that inhibit or kill competing microbial species Antimicrobial drugs must display selective toxicity,
More informationGuidance for completing the OIE template for the collection of data on antimicrobial agents intended for use in animals
Guidance for completing the OIE template for the collection of data on antimicrobial agents intended for use in animals Contents Introduction... 1 Required information and choices for reporting... 2 Baseline
More informationManureTracker: On the Trail of Hormones, Antimicrobials and Antimicrobial Resistance Genes
ManureTracker: On the Trail of Hormones, Antimicrobials and Antimicrobial Resistance Genes Francis J. Larney 1, Srinivas Sura 2, Shanwei Xu 1, Edward Topp 2, and Tim A. McAllister 1 1 Agriculture & Agri-Food
More informationIntroduction to Pharmacokinetics and Pharmacodynamics
Introduction to Pharmacokinetics and Pharmacodynamics Diane M. Cappelletty, Pharm.D. Assistant Professor of Pharmacy Practice Wayne State University August, 2001 Vocabulary Clearance Renal elimination:
More informationPharm 262: Antibiotics. 1 Pharmaceutical Microbiology II DR. C. AGYARE
Pharm 262: 1 Pharmaceutical Microbiology II Antibiotics DR. C. AGYARE Reference Books 2 HUGO, W.B., RUSSELL, A.D. Pharmaceutical Microbiology. 6 th Ed. Malden, MA: Blackwell Science, 1998. WALSH, G. Biopharmaceuticals:
More informationIntroduction to Chemotherapeutic Agents. Munir Gharaibeh MD, PhD, MHPE School of Medicine, The university of Jordan November 2018
Introduction to Chemotherapeutic Agents Munir Gharaibeh MD, PhD, MHPE School of Medicine, The university of Jordan November 2018 Antimicrobial Agents Substances that kill bacteria without harming the host.
More informationAMR dissemination in the environment Professor Liz Wellington
AMR dissemination in the environment Professor Liz Wellington The connectivity of potential sources of antibioticresistant bacteria Antibiotic resistance in the environment: soil, sediments, water bodies
More informationScreening 36 Veterinary Drugs in Animal Origin Food by LC/MS/MS Combined with Modified QuEChERS Method
Screening 36 Veterinary Drugs in Animal Origin Food by LC/MS/MS Combined with Modified QuEChERS Method Application Note Food Testing and Agriculture Authors Jin-Lan Sun, Chang Liu, Yue Song Agilent Technologies
More informationFACT SHEETS. On the Danish restrictions of non-therapeutical use of antibiotics for growth promotion and its consequences
12 July 2010 FACT SHEETS On the Danish restrictions of non-therapeutical use of antibiotics for growth promotion and its consequences Denmark is a major livestock producer in Europe, and the worlds largest
More informationThe epidemiology of antimicrobial resistance and the link between human and veterinary medicine
The epidemiology of antimicrobial resistance and the link between human and veterinary medicine Prof. Dr. Jeroen Dewulf Jeroen.Dewulf@UGent.be Unit for Veterinary Epidemiology, Faculty of Veterinary Medicine
More informationSurveillance for antimicrobial resistance in enteric bacteria in Australian pigs and chickens
Surveillance for antimicrobial resistance in enteric bacteria in Australian pigs and chickens Dr Pat Mitchell R & I Manager Production Stewardship APL CDC Conference, Melbourne June 2017 Dr Kylie Hewson
More informationVeterinary Feed Directive Information
Veterinary Feed Directive Information Focus and Scope Veterinary- Patient-Client Relationship Veterinarian issuing a VFD is required to be licensed to practice veterinary medicine and operate in compliance
More informationAntimicrobial Therapy
Chapter 12 The Elements of Chemotherapy Topics - Antimicrobial Therapy - Selective Toxicity - Survey of Antimicrobial Drug - Microbial Drug Resistance - Drug and Host Interaction Antimicrobial Therapy
More informationcrippling production of the bacterial cell wall that protects the cell from the external environment PS
Antibiotic Selection and Use in Cattle Dee Griffin DVM MS, Texas A&M Veterinary Medical Center, Canyon, TX 79016 Antibiotic use in food animals is increasingly scrutinized Much of the world s antibiotic
More informationOccurrence of Pharmaceuticals, Hormones, and Organic Wastewater Compounds in Pennsylvania Waters
Occurrence of Pharmaceuticals, Hormones, and Organic Wastewater Compounds in Pennsylvania Waters U.S. Geological Survey Scientific Investigations Report 2012-5106 Background Pharmaceuticals, Hormones,
More informationANTIMICROBIAL USAGE IN AQUACULTURE
FMM/RAS/298: Strengthening capacities, policies and national action plans on prudent and responsible use of antimicrobials in fisheries ANTIMICROBIAL USAGE IN AQUACULTURE Review of AMU in aquaculture based
More informationMicrobiology : antimicrobial drugs. Sheet 11. Ali abualhija
Microbiology : antimicrobial drugs Sheet 11 Ali abualhija return to our topic antimicrobial drugs, we have finished major group of antimicrobial drugs which associated with inhibition of protein synthesis
More informationAntimicrobial use in poultry: Emerging public health problem
Antimicrobial use in poultry: Emerging public health problem Eric S. Mitema, BVM, MS, PhD CPD- Diagnosis and Treatment of Poultry Diseases FVM, CAVS, 6 th. August, 2014 AMR cont Antibiotics - Natural or
More informationUsing Charm II 7600 For Residue Testing in Meats in Barbados
Using Charm II 7600 For Residue Testing in Meats in Barbados Dr Trevor King/ June Roach, Vet Services Lab (VSL) Ministry of Agriculture & Rural Development, Barbados VSL s Client Base Poultry In dustry
More informationAntibiotic Residues in Meat and Meat Products, Implications on Human Health
Antibiotic Residues in Meat and Meat Products, Implications on Human Health Loinda Rugay Baldrias, DVM, MVS, PhD Dean, Professor College of Veterinary Medicine University of the Philippines Los Banos National
More informationa. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2.
AND QUANTITATIVE PRECISION (SAMPLE UR-01, 2017) Background and Plan of Analysis Sample UR-01 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony
More informationJanuary 2014 Vol. 34 No. 1
January 2014 Vol. 34 No. 1. and Minimum Inhibitory Concentration (MIC) Interpretive Standards for Testing Conditions Medium: diffusion: Mueller-Hinton agar (MHA) Broth dilution: cation-adjusted Mueller-Hinton
More informationChanging Practices to Reduce Antibiotic Resistance
Changing Practices to Reduce Antibiotic Resistance Jean E. McLain, Research Scientist and Assistant Dean University of Arizona College of Agriculture and Life Sciences and Department of Soil, Water and
More informationSome Antibacterial Agents Used with Koi (oz refers to weight unless otherwise specified)
Some Antibacterial Agents Used with Koi (oz refers to weight unless otherwise specified) (Note: many chemicals have been used at one time or another to combat bacterial disease in koi. We have attempted
More informationLab Exercise: Antibiotics- Evaluation using Kirby Bauer method.
Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method. OBJECTIVES 1. Compare the antimicrobial capabilities of different antibiotics. 2. Compare effectiveness of with different types of bacteria.
More informationAntimicrobials. Antimicrobials
Antimicrobials For more than 50 years, antibiotics have come to the rescue by routinely producing rapid and long-lasting miracle cures. However, from the beginning antibiotics have selected for resistance
More informationApproach to pediatric Antibiotics
Approach to pediatric Antibiotics Gassem Gohal FAAP FRCPC Assistant professor of Pediatrics objectives To be familiar with common pediatric antibiotics o Classification o Action o Adverse effect To discus
More informationAnimal Antibiotic Use and Public Health
A data table from Nov 2017 Animal Antibiotic Use and Public Health The selected studies below were excerpted from Pew s peer-reviewed 2017 article Antimicrobial Drug Use in Food-Producing Animals and Associated
More informationMonitoring of Antibiotic Residues in Milk Development of the New Biosensor System MCR 3 for Routine Practical Analyses
Milchprüfring Bayern e.v. Monitoring of Antibiotic Residues in Milk Development of the New Biosensor System MCR 3 for Routine Practical Analyses Innovation Networking Transparency Information Milchprüfring
More informationBUGS and DRUGS Part 1 March 6, 2013 Marieke Kruidering- Hall
BUGS and DRUGS Part 1 March 6, 2013 Marieke Kruidering- Hall BIOGRAPHY: Marieke Kruidering- Hall is Associate Professor in the Department of Cellular & Molecular Pharmacology. She was born in the Netherlands.
More informationEurEau s Contribution to the European Commission s Strategic Approach on Veterinary Pharmaceuticals in the Environment
EurEau s Contribution to the European Commission s Strategic Approach on Veterinary Pharmaceuticals in the Environment Summary Globally, pharmaceutical products are regularly administered to both livestock
More informationIn Vitro Susceptibility of Brucella
APPuED MICROBIOLOGY, Oct. 1970, p. 600-604 Vol. 20, No. 4 Copyright 1970 American Society for Microbiology Printed in U.S.A. In Vitro Susceptibility of Brucella to Various Antibiotics WENDELL H. HALL AND
More informationMulti-residue Method I for Veterinary Drugs by HPLC (Animal and Fishery Products)
Multi-residue Method I for Veterinary Drugs by HPLC (Animal and Fishery Products) 1. Analytes See Table 7. 2. Instruments High performance liquid chromatograph-photodiode array detector (HPLC-DAD) High
More informationARCH-Vet. Summary 2013
Federal Department of Home Affairs FDHA FSVO ARCH-Vet Report on sales of antibiotics in veterinary medicine and antibiotic resistance monitoring of livestock in Switzerland Summary 2013 Published by Federal
More informationAgricultural Research Division, American Cyanamid Company, Princeton, NJ 08540
1 Antibiotics Use in Agriculture: An Overview Richard H. Gustafson Downloaded via 148.251.232.83 on October 16, 2018 at 00:12:00 (UTC). See https://pubs.acs.org/sharingguidelines for options on how to
More informationIs ABF production supporting or contradicting bird welfare? Maarten De Gussem, DVM Poultry Vaccinology - Ceva Summit March 14th 2016, Barcelona
Is ABF production supporting or contradicting bird welfare? Maarten De Gussem, DVM Poultry Vaccinology - Ceva Summit March 14th 2016, Barcelona Overview 2 Scope and introduction Why do we use antitibiotics
More informationShort Report. R Boot. Keywords: Bacteria, antimicrobial susceptibility testing, quality, diagnostic laboratories, proficiency testing
Short Report Frequent major errors in antimicrobial susceptibility testing of bacterial strains distributed under the Deutsches Krebsforschungszentrum Quality Assurance Program R Boot Former Section of
More informationPipestone Veterinary Services
NIAA: 2017 Antibiotic Symposium Oct 31 Nov 2, 2017 Joel Nerem, DVM Pipestone Veterinary Services 5 Locations Pipestone, MN Independence, IA Ottumwa, IA DeKalb, IL Rensselaer, IN Mixed Animal Practice.
More informationCountry Report Myanmar
Country Report Myanmar Pyi Sone 1 and Ye Htut Aung 2 1 General Manager Livestock, Feedstuff and Milk Products Enterprise 2 Professor, Department of Medicine, University of Veterinary Science, Yezin, Myanmar
More informationObjectives. Antibiotics uses in food animals 3/25/2018. California Dairy Productions. Antimicrobial Resistance in the Animal Production Environment
Antimicrobial Resistance in the Animal Production Environment Xunde Li Western Institute for Food Safety and Security Department of Population Health and Reproduction University of California Davis Objectives
More informationIntroduction PAPER IN FOREFRONT. Larissa J. M. Jansen 1 & Yvette J. C. Bolck 1 & Janneau Rademaker 1 & Tina Zuidema 1 & Bjorn J. A.
Anal Bioanal Chem (2017) 409:4927 4941 DOI 10.1007/s00216-017-0445-0 PAPER IN FOREFRONT The analysis of tetracyclines, quinolones, macrolides, lincosamides, pleuromutilins, and sulfonamides in chicken
More informationMilk and Dairy Beef Residues: Incidence & Communications. Dairy Response Planning Betsy Flores, Nat l Milk Producers Federation April 16, 2013
Milk and Dairy Beef Residues: Incidence & Communications Dairy Response Planning Betsy Flores, Nat l Milk Producers Federation April 16, 2013 What We ll Cover Antibiotics and milk production FDA drug residue
More informationANTIMICROBIAL STEWARDSHIP
ANTIMICROBIAL STEWARDSHIP Australian Pig Veterinarians Annual Conference 11-12 September, 2017 Stephen Page Advanced Veterinary Therapeutics swp@advet.com.au OUTLINE Importance of AMR Antimicrobial use
More informationExtraction and Cleanup Protocols for LC-MS/MS Multiresidue Determination of Veterinary Drugs in Tissue and Milk Samples
Extraction and Cleanup Protocols for LC-MS/MS Multiresidue Determination of Veterinary Drugs in Tissue and Milk Samples Malin Wangler, Waters Sweden Michael S. Young and Kim vantran Waters Milford 2011
More informationExploring simvastatin, an antihyperlipidemic drug, as a potential topical antibacterial agent
Supplementary materials Exploring simvastatin, an antihyperlipidemic drug, as a potential topical antibacterial agent Shankar Thangamani 1, Haroon Mohammad 1, Mostafa Abushahba 1, Maha Hamed 1, Tiago Sobreira
More informationMulti-residue Determination of Polar Veterinary Drugs in Livestock and Fishery Products by Liquid Chromatography/ Tandem Mass Spectrometry
23 Kanda et al.: Journal of AOAC International Vol. 98, No. 1, 215 VETERINARY DRUG RESIDUES Multi-residue Determination of Polar Veterinary Drugs in Livestock and Fishery Products by Liquid Chromatography/
More informationFrank Møller Aarestrup
Danish Veterinary Laboratory Bacterial populations and resistance development: Intestinal tract of meat animals Frank Møller Aarestrup 12 Antibiotic production 10 Mill. Kg 8 6 4 2 0 50 52 54 56 58 60 62
More informationProceedings of the 13th International Congress of the World Equine Veterinary Association WEVA
www.ivis.org Proceedings of the 13th International Congress of the World Equine Veterinary Association WEVA October 3-5, 2013 Budapest, Hungary Reprinted in IVIS with the Permission of the WEVA Organizers
More informationResult of the OIE data collection
Delfy Góchez Chargée de mission Result of the OIE data collection Lessons learned and expectations Beirut, November 8th 2017 Reporting Options The sections of the OIE Template named Reporting Options 1,
More informationRoutine internal quality control as recommended by EUCAST Version 3.1, valid from
Routine internal quality control as recommended by EUCAST Version.1, valid from 01-01-01 Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus
More informationDANMAP Danish Integrated Antimicrobial Resistance Monitoring and Research Programme
DANMAP Danish Integrated Antimicrobial Resistance Monitoring and Research Programme Hanne-Dorthe Emborg Department of Microbiology and Risk Assessment National Food Institute, DTU Introduction The DANMAP
More informationAntibacterial susceptibility testing
Antibiotics: Antil susceptibility testing are natural chemical substances produced by certain groups of microorganisms (fungi, ) that inhibit the growth of or kill the other that cause infection. Several
More informationChapter 12. Antimicrobial Therapy. Antibiotics 3/31/2010. Spectrum of antibiotics and targets
Chapter 12 Topics: - Antimicrobial Therapy - Selective Toxicity - Survey of Antimicrobial Drug - Microbial Drug Resistance - Drug and Host Interaction Antimicrobial Therapy Ehrlich (1900 s) compound 606
More informationThe Basics: Using CLSI Antimicrobial Susceptibility Testing Standards
The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards Janet A. Hindler, MCLS, MT(ASCP) UCLA Health System Los Angeles, California, USA jhindler@ucla.edu 1 Learning Objectives Describe information
More informationDetermination, Confirmation and Quantitation of Multi-Class Antibiotic Residues in Milk by UHPLC MS/MS
APPLICATION NOTE Liquid Chromatography/ Mass Spectrometry Authors: Avinash Dalmia PerkinElmer, Inc. Shelton, CT Determination, Confirmation and Quantitation of Multi-Class Antibiotic Residues in Milk by
More informationAntibiotics in vitro : Which properties do we need to consider for optimizing our therapeutic choice?
Antibiotics in vitro : Which properties do we need to consider for optimizing our therapeutic choice? With the support of Wallonie-Bruxelles-International 1-1 In vitro evaluation of antibiotics : the antibiogram
More informationAntimicrobial agents
Bacteriology Antimicrobial agents Learning Outcomes: At the end of this lecture, the students should be able to: Identify mechanisms of action of antimicrobial Drugs Know and understand key concepts about
More informationUltra-Fast Analysis of Contaminant Residue from Propolis by LC/MS/MS Using SPE
Ultra-Fast Analysis of Contaminant Residue from Propolis by LC/MS/MS Using SPE Matthew Trass, Philip J. Koerner and Jeff Layne Phenomenex, Inc., 411 Madrid Ave.,Torrance, CA 90501 USA PO88780811_L_2 Introduction
More informationAntibiotic Resistance in Bacteria
Antibiotic Resistance in Bacteria Electron Micrograph of E. Coli Diseases Caused by Bacteria 1928 1 2 Fleming 3 discovers penicillin the first antibiotic. Some Clinically Important Antibiotics Antibiotic
More informationVeterinary Feed Directive: What You Need to Know
Iowa Farm Bureau s Margin Management Webinar Series presents: Veterinary Feed Directive: What You Need to Know Are you prepared for implementation of the Veterinary Feed Directive on January 1, 2017? Introduction:
More informationSuggestions for appropriate agents to include in routine antimicrobial susceptibility testing
Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing These suggestions are intended to indicate minimum sets of agents to test routinely in a diagnostic laboratory
More informationEUCAST recommended strains for internal quality control
EUCAST recommended strains for internal quality control Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus influenzae ATCC 59 ATCC
More informationConsumption of antibiotics in hospitals. Antimicrobial stewardship.
Consumption of antibiotics in hospitals. Antimicrobial stewardship. Inge C. Gyssens MD PhD Radboud university medical center, Nijmegen, The Netherlands Hasselt University, Belgium 1. Antibiotic use in
More informationDR. BASHIRU BOI KIKIMOTO
OVERVIEW OF ANTIMICROBIAL RESISTANCE AND ANTIMICROBIAL USE IN GHANA PRESENTED BY : DR. BASHIRU BOI KIKIMOTO DVM. PhD VETERINARY PUBLIC HEALTH HEAD - PUBLIC HEALTH UNIT & FOOD SAFETY UNIT VENUE: SWATZILAND
More informationAn#bio#cs and challenges in the wake of superbugs
An#bio#cs and challenges in the wake of superbugs www.biochemj.org/bj/330/0581/bj3300581.htm ciss.blog.olemiss.edu Dr. Vassie Ware Bioscience in the 21 st Century November 14, 2014 Who said this and what
More informationAOAC SMPR Intended Use: Routine Surveillance for GMP Compliance
AOAC SMPR 2018.010 Standard Method Performance Requirements (SMPRs ) for Screening and Identification Method for Regulated Veterinary Drug Residues in Food Intended Use: Routine Surveillance for GMP Compliance
More informationHSE - Health Protection Surveillance Centre Surveillance of Antimicrobial Consumption in Ireland
Surveillance of Antimicrobial Consumption in Ireland Ajay Oza A European Study on the Relationship between Antimicrobial Use and Antimicrobial Resistance (1998-1999) Bronzwaer et al 2002 Emerging Infectious
More information56 Clinical and Laboratory Standards Institute. All rights reserved.
Table 2C 56 Clinical and Laboratory Standards Institute. All rights reserved. Table 2C. Zone Diameter and Minimal Inhibitory Concentration Breakpoints for Testing Conditions Medium: Inoculum: diffusion:
More informationDAIRY VETERINARY NEWSLETTER
DAIRY VETERINARY NEWSLETTER Drought Year Issues Include Aflatoxin Testing and Nitrate Testing of Feed Aflatoxins in corn and other feed crops during drought conditions September 2012 2012 is shaping up
More informationAntibacterial therapy 1. د. حامد الزعبي Dr Hamed Al-Zoubi
Antibacterial therapy 1 د. حامد الزعبي Dr Hamed Al-Zoubi ILOs Principles and terms Different categories of antibiotics Spectrum of activity and mechanism of action Resistancs Antibacterial therapy What
More informationQuality Control Testing with the Disk Antibiotic Susceptibility Test of Bauer-Kirby-Sherris-Turck
Quality Control Testing with the Disk Antibiotic Susceptibility Test of Bauer-Kirby-Sherris-Turck DONNA J. BLAZEVIC, M.P.H., MARILYN H. KOEPCKE, B.S., A JOHN M. MATSEN, M.D. Departments of Laboratory Medicine
More informationAntibiotic Spectral Library Version 1.1 for LibraryView Software
Antibiotic Spectral Library Version 1.1 for LibraryView Software Antibiotic Spectral Library v1.1 for LibraryView Software An overview of the licensed mass spectral library for antibiotics compatible with
More information