Routine internal quality control as recommended by EUCAST Version 3.1, valid from
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1 Routine internal quality control as recommended by EUCAST Version.1, valid from Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus influenzae Campylobacter jejuni ATCC 59 ATCC 785 ATCC 91 ATCC 91 ATCC 9619 NCTC ATCC 560 Version number Strain/page Changes Cells containing a change or an addition from v.0 are marked blue..1, First page Typo corrected for Campylobacter jejuni. The correct ATCC number is 560. ATCC 59 Typo corrected on ceftaroline range changed to Version number Strain/page Changes Cells containing a change or an addition from v.1 are marked yellow. ATCC 59 QC ranges updated : Cefalexin (zone). QC ranges added: Ceftaroline ( and zone). ATCC 91 QC ranges added: Ceftaroline ( and zone)..0, ATCC 9619 Clarification regarding differentiation between haemolysis and growth. QC ranges added: Ceftaroline (). NCTC 868 QC ranges added: Ceftaroline (range to be established). ATCC 560 New QC strain. All ranges new. 1 NCTC 868 is susceptible to beta-lactam antibiotics and often easier to read on MH-F than the commonly recommended ATCC 97 showing variable inhibition zones for beta-lactam antibiotics due to PBP mutations. Updated by EUCAST.
2 Escherichia coli ATCC 59 (NCTC 11, CIP 76., DSM 110, CCUG 1760, CECT ) Mueller-Hinton agar, McFarland 0.5, air, 5±1ºC, 18±h. Read zone edges as the point showing no growth viewed from the back of the plate against a dark background illuminated with reflected light. Target 1 Range (µg) Amikacin Amoxicillin Amoxicillin-clavulanic acid / /1-8/ Ampicillin Ampicillin-sulbactam / /1-8/ Aztreonam Cefadroxil Cefalexin Cefepime Cefixime Cefotaxime Cefoxitin Cefpodoxime Ceftaroline Ceftazidime Ceftibuten Ceftriaxone Cefuroxime Chloramphenicol Ciprofloxacin Colistin Doripenem Ertapenem Fosfomycin Gentamicin Imipenem Levofloxacin Mecillinam Meropenem Moxifloxacin Nalidixic acid Netilmicin Nitrofurantoin Norfloxacin Ofloxacin Piperacillin Piperacillin-tazobactam / 1/-/ Ticarcillin Ticarcillin-clavulanic acid 8/ /-16/ Tigecycline Tobramycin Trimethoprim Trimethoprim-sulfamethoxazole 0.5/ From International Standards Organisation, ISO : 006, except ranges in bold/italics established by EUCAST. From Clinical and Laboratory Standards Institute, M100-S: :, 01, except ranges in bold/italics established by EUCAST. All ranges have been validated by EUCAST. Ranges and targets are valid for a :1 ratio of beta-lactam agent to beta-lactamase inhibitor. EUCAST is currently collecting data to establish control ranges with fixed beta-lactamase inhibitor concentrations, in accordance with EUCAST breakpoints. 5 Ignore growth that may appear as a thin inner zone on some batches of Mueller-Hinton agars. 6 For test conditions, see Clinical and Laboratory Standards Institute, M100-S: :, 01.
3 Pseudomonas aeruginosa ATCC 785 (NCTC 190, CIP , DSM 1117, CCUG 17619, CECT 108) Mueller-Hinton agar, McFarland 0.5, air, 5±1ºC, 18±h. Read zone edges as the point showing no growth viewed from the back of the plate against a dark background illuminated with reflected light. Target 1 Range (µg) Amikacin Aztreonam Cefepime Ceftazidime Ciprofloxacin Colistin Doripenem Fosfomycin Gentamicin Imipenem Levofloxacin Meropenem Netilmicin Piperacillin Piperacillin-tazobactam -/ 1/-8/ Ticarcillin Ticarcillin-clavulanic acid 16/ 8/-/ Tobramycin From International Standards Organisation, ISO : 006. From Clinical and Laboratory Standards Institute, M100-S: :, 01, except ranges in bold/italics established by EUCAST. All ranges have been validated by EUCAST. For test conditions, see Clinical and Laboratory Standards Institute, M100-S: :, 01.
4 Staphylococcus aureus ATCC 91* (NCTC 197, CIP 109, DSM 569, CCUG 15915, CECT 79) * β-lactamase-producing strain (weak) Mueller-Hinton agar, McFarland 0.5, air, 5±1ºC, 18±h. Read zone edges as the point showing no growth viewed from the back of the plate against a dark background illuminated with reflected light. Target 1 Range (µg) Amikacin Ampicillin Azithromycin Benzylpenicillin unit Cefoxitin Ceftaroline Chloramphenicol Ciprofloxacin Clarithromycin Clindamycin Daptomycin Doxycycline Erythromycin Fosfomycin Fusidic acid Gentamicin Levofloxacin Linezolid Minocycline Moxifloxacin Mupirocin Netilmicin Nitrofurantoin Norfloxacin Ofloxacin Quinupristin-dalfopristin Rifampicin Teicoplanin Telavancin Tetracycline Tigecycline Tobramycin Trimethoprim Trimethoprim-sulfamethoxazole 0.5/ Vancomycin From International Standards Organisation, ISO : 006. From Clinical and Laboratory Standards Institute, M100-S: :, For test conditions, see Clinical and Laboratory Standards Institute, M100-S: :, 01.
5 Enterococcus faecalis ATCC 91 (NCTC 1697, CIP 101, DSM 570, CCUG 9997, CECT 795) Mueller-Hinton agar, McFarland 0.5, air, 5±1ºC, 18±h. Read zone edges as the point showing no growth viewed from the back of the plate against a dark background illuminated with reflected light. Target 1 Range (µg) Ampicillin Gentamicin Imipenem Linezolid Nitrofurantoin Quinupristin-dalfopristin Teicoplanin Tigecycline Trimethoprim Trimethoprim-sulfamethoxazole 0.5/ Vancomycin From International Standards Organisation, ISO : 006. Screening disk for high-level aminoglycoside-resistance in enterococci. 5 From Clinical and Laboratory Standards Institute, M100-S: :, For test conditions, see Clinical and Laboratory Standards Institute, M100-S: :, 01.
6 Streptococcus pneumoniae ATCC 9619*/** (NCTC 1977, CIP 100, DSM 11967, CCUG 68) * Penicillin-intermediate strain ** Zone edges for S. pneumoniae on MH-F are often accompanied by α-haemolysis. Read inhibition of growth and not inhibition of haemolysis. Tilt the plate to easier differentiate between haemolysis and growth. There is usually growth in the whole area of α-haemolysis but on some MH-F media, there is additional α-haemolysis without growth. Mueller-Hinton agar + 5% horse blood and 0 mg/l β-nad, McFarland 0.5, 5% CO, 5±1ºC, 18±h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid removed and with reflected light. Target 1 Range (µg) Amoxicillin Ampicillin Azithromycin Benzylpenicillin unit Cefaclor Cefepime Cefotaxime Cefpodoxime Ceftaroline IP IP Ceftriaxone Cefuroxime Chloramphenicol Ciprofloxacin Clarithromycin Clindamycin Daptomycin Doripenem Doxycycline Ertapenem Erythromycin Imipenem Levofloxacin Linezolid Meropenem Minocycline Moxifloxacin Nitrofurantoin Norfloxacin Ofloxacin Oxacillin Rifampicin Teicoplanin Telithromycin Tetracycline Tigecycline Trimethoprim-sulfamethoxazole 0.5/ / /.-1/ Vancomycin From International Standards Organisation, ISO : 006. Clinical and Laboratory Standards Institute, M100-S: :, For test conditions, see Clinical and Laboratory Standards Institute, M100-S: :, 01. IP = In Preparation
7 Haemophilus influenzae NCTC 868 (CIP 5.9, CCUG 96) Mueller-Hinton agar + 5% horse blood and 0 mg/l β-nad, McFarland 0.5, 5% CO, 5±1ºC, 18±h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid removed and with reflected light. (µg) Target Range Target 1 Range Amoxicillin-clavulanic acid IP IP Ampicillin IP IP 19-5 Benzylpenicillin IP IP 1 unit Cefaclor IP IP Cefepime IP IP Cefixime IP IP Cefotaxime IP IP Cefpodoxime IP IP Ceftaroline IP IP 5 IP IP Ceftibuten IP IP Ceftriaxone IP IP Cefuroxime IP IP Chloramphenicol IP IP Ciprofloxacin IP IP Doripenem IP IP Ertapenem IP IP Erythromycin IP IP Imipenem IP IP Levofloxacin IP IP Meropenem IP IP Minocycline IP IP Moxifloxacin IP IP Nalidixic acid IP IP Ofloxacin IP IP Rifampicin IP IP Telithromycin IP IP Tetracycline IP IP Trimethoprim-sulfamethoxazole IP IP IP = In Preparation
8 Campylobacter jejuni ATCC 560 (NCTC 1151, CIP 70, DSM 688, CCUG 118) Mueller-Hinton agar + 5% horse blood and 0 mg/l β-nad, McFarland 0.5, microaerobic environment, 1±1ºC, h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid removed and with reflected light. The MH-F plates should be dried prior to inoculation to reduce swarming (at 0-5 C over night or at 5 C, with the lid removed, for 15 min). (µg) Target Range Target 1 Range Ciprofloxacin IP IP Erythromycin IP IP Tetracycline IP IP IP = In Preparation
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