Veterinary Diagnostic Services Price List February 2018

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1 Veterinary Diagnostic Services Price List February 2018

2 Feline Infectious Diseases Retroviruses FeLV antigen Respiratory Positive results on our ELISA are confirmed FOC FeLV virus isolation WB will be used where required alongside IF to ensure positive results agree using 2 different methods FIV antibodies: Western blot Feline retrovirus profile (FeLV antigen & FIV antibodies) FeLV, FIV, FCoV Virus isolation from tissues (Feline cowpoxvirus, feline herpesvirus, feline calicivirus) To confirm in-clinic positive test FeLV antigen + virus isolation FeLV antigen + qpcr FeLV antigen + qpcr + virus isolation FIV antibodies: IFA (Feline herpesvirus, feline calicivirus) To confirm in-clinic positive test FeLV qpcr Respiratory virus isolation from swabs FHV real-time PCR Chlamydophila felis PCR Bordetella bronchiseptica (C&S; part profile) Chlamydophila felis PCR + FHV PCR Respiratory virus isolation + FHV PCR Respiratory virus isolation + C. felis PCR Triple respiratory profile (Virus isolation, FHV PCR, C. felis PCR) Feline conjunctivitis profile (FHV PCR, C. felis PCR, Mycoplasma culture & M. felis PCR) (FeLV antigen, FIV & FCoV antibodies) Other Disease Profiles Gingivitis profile FIP FIP profile (FCoV, α1-agp, A:G, haematology/ cytology) FCoV antibodies + α1-agp FCoV antibodies α1-agp FCoV antibodies + FCoV RT-PCR FCoV RT-PCR Pyrexic cat profile (FeLV, FIV, FCoV, haematology, ALP, ALT) Feline uveitis profile Feline neurological profile Feline parasitology screen Other Tests (FPV PCR and FPV antibodies) FCV plaque typing To distinguish field & vaccine FCV; FCV must be isolated first Parasitology Toxoplasma gondii antibodies Giardia lamblia PCR Tritrichomonas foetus PCR Protozoan screen Cryptosporidium spp Aelurostrongylus abstrusus Nematode & cestode egg screen (Diarrhoea profile & Crypto, FCoV PCR, Giardia PCR, T. foetus PCR) (Nematodes, cestodes, protozoa, Cryptosporidium) Parvovirus Feline parvovirus antibodies Feline parvovirus PCR Feline diarrhoea profile plus (FeLV, FIV, FCoV, Toxoplasma) (RT-PCR on faeces, effusion) Feline parvovirus profile (FeLV, FIV, FCoV, Toxoplasma) (FNA, tissue) FIP profile + FCoV RT-PCR Feline diarrhoea profile (Bacteriology, parasitology, not Cryptosporidium) (faeces, effusion) FCoV RT-PCR (Resp. virus isolation, FeLV, FIV) FeLV virus neutralising antibodies NOT for demonstrating immunity post vaccination Bacteriology & Mycology Full culture and sensitivity Aerobic culture and sensitivity (urine, ear) Enrichment culture (blood, joint, CSF) Fungal culture including Dermatophytes Mycoplasma culture and M. felis real-time PCR Mycoplasma spp PCR (not for routine screening) Clostridium perfringens cpe gene PCR (on isolates)

3 Canine Infectious Diseases Does the dog need a vaccine booster? CPV, CDV and CAV antibodies Disease Profiles Canine parvovirus profile (CPV PCR and CPV antibodies) Canine diarrhoea profile (bacteriology and parasitology, not Cryptosporidium) Canine diarrhoea profile plus Diagnostic testing of sick dogs Canine parvovirus (CPV) antibodies Submit paired sera in sick vaccinated dogs ( 27.50) Canine parvovirus PCR Canine coronavirus (CCV) antibodies Canine coronavirus PCR Canine parainfluenza virus (CPiV) antibodies Not for demonstrating immunity post vaccination. Submit paired Canine parainfluenza virus isolation Canine adenovirus (CAV) antibodies Canine respiratory virus isolation (CPiV, CAV2, CHV) Canine respiratory profile (Bordetella culture, resp. virus isolation, CPiV serology) Canine blue eye profile (CAV1 & CAV2 serology) Canine neurological profile (Neospora serology, Toxoplasma serology) Canine parasitology screen sera in sick vaccinated dogs () (diarrhoea profile & Crypto, Giardia PCR, CCV PCR) (nematodes, cestodes, protozoa, Cryptosporidium) Specify CAV-1 or CAV-2. Select both CAV1 & CAV2 in sick Bacteriology & Mycology vaccinated dogs ( ) Canine adenovirus isolation Canine herpesvirus (CHV) antibodies Not for demonstrating immunity post vaccination Canine herpesvirus isolation Canine distemper virus (CDV) antibodies Submit paired sera in sick vaccinated dogs ( ) Leptospira antibodies (serovars Icterohaemorrhagiae, Canicola, Bratislava, Hardjo) Full culture and sensitivity Aerobic culture and sensitivity (urine, ear) Enrichment culture (blood, joint, CSF) Fungal culture including Dermatophytes Mycoplasma cynos PCR (BAL, tracheal washes) Mycoplasma spp PCR (not for routine screening- please contact us to discuss) Clostridium perfringens cpe gene PCR (clinical isolates) Not for demonstrating immunity post vaccination Parasitology Toxoplasma gondii antibodies Neospora caninum antibodies Giardia lamblia PCR Protozoan screen Cryptosporidium spp Nematode & cestode egg screen Angiostrongylus vasorum Ectoparasite screen (skin scrape) New Molecular Testing now available Pathogen Species Bordetella bronchiseptica Mycoplasma cynos Mycoplasma genus Encephalitozoon cuniculi Ferret coronavirus C. perfringens cpe toxin Cat, Dog Dog All species Rabbit Ferret All species

4 Clinical Pathology Biochemistry (Heparin blood or Serum/Plasma) * Full biochemistry profile: Endocrinology (Serum) As Routine plus Glucose (Include Fluoride Oxalate sample For glucose) * Basic biochemistry profile: Total Protein, Alb, Alk Phos. ALT, Urea, Creatinine * Routine biochemistry profile (Dogs & Cats): As Basic plus Albumin, Globulin, AST, GGT, Bilirubin, Electrolytes, ACTH stimulation test (2x Cortisol) DEX suppression test (3x Cortisol) T4 (state time if post pill) CANINE TSH 'One off' cortisol (resting) /29.00** /16.00** **Discounted price for subsequent therapeutic monitoring Calcium, Phosphate, Cholesterol, Triglyceride Electrolyte profile: (Serum or Heparin) Sodium, Potassium & Chloride & Na:K Ratio Urinalysis Full urine analysis: Full Urinalysis: Qualitative, Quantitative & Deposit Deposit examination: Additional Full Haematology When included with profiles marked with an asterisk (*) RBC, WBC, epithelial cells, sperm, organisms, cell debris, yeasts & casts Add Qualitative (dipstick) analysis: Qualitative protein, glucose, ketones, haemoglobin, ph, specific gravity Qualitative analysis: Protein, Creatinine, Prot:Creat ratio Haematology (EDTA blood sample and smears) Full haematology including smear examination: RBC, Hct, Hb, MCV, MCH, MCHC, RDW, WBC & Differential count, Full fluid analysis: Platelet count Coagulation screen: (Citrated Blood) Fibrinogen, PT, KCCT Blood smear examination only: Cytological examination of submitted (air-dried) smear including Cell counts, Proteins & Cytology, inc. cytologists report Fluid proteins: Total Protein, Albumin, Globulin, A:G ratio Fluid cytology (e.g. lavages) Cell counts, smear exam examination for blood parasites CSF profile (CSF fluid in EDTA/Plain tube) Fluid Analysis Fluid cholesterol/tryglyceride Cell count, Differential, cytospin examination & Protein Cytology FNA cytology (1st site) including cytologists report: Biochemistry Profiles * Liver profile 1: (Serum) Total protein, Albumin, Globulin, Alkaline phosphatase, ALT, Bilirubin * Liver profile 2: (Serum Only) Urea, Creatinine, Triglyceride, Amylase, Lipase * Equine profile: Total Protein, Alb, Glob, Alk Phos, GGT, AST, Bilirubin, Urea, Creatinine, Calcium, Phosphate, Electrolytes, Triglyceride, Creatine Kinase * Downer cow profile: Calcium, Phosphate, Magnesium, Creatine Kinase, Urea Diabetic monitoring: Serum or Heparin for Fructosamine; Fluoride Oxalate for Glucose * Kindney profile 1 (Serum/Plasma + Urine) Total Protein, Albumin, Globulin, Urea, Creatine, Phosphate & Urine Specific Gravity * Kindney profile as Kidney 1 plus Calcium, Electrolytes, Cholesterol * Hyperthyroid cat profile: (Serum) Total Prot., Alb, Glob, Alk.Phos, ALT, Urea, Creatinine, T4, Phosphate * Ruminant profile: Total Prot. Alb, Glob, Alk.Phos, GGT, AST, GLDH, Bilirubin, Urea, Creatinine, Calcium, Phosphate, Magnesium, Electrolytes * T4/canine TSH profile (Serum or Heparin) State time if T4 post pill FNA cytology additional sites (each) as Liver 1 plus GGT, AST, Bile Acids resting & postprandial * Pancreatic profile FNA, Touch Prep or submitted smears (air-dried) Additional Tests Single biochemistry tests each unless otherwise stated. Bile acids resting, pre or post prandial (each) (Serum) Troponin Fibrinogen (Citrated Blood) FDP s (Citrated Blood) Reticulocyte count Fructosamine Bile Acid Stim (x3 samples) Pre stim, 1hr post stim, 2hr post stim Phenobarbitone (serum NOT gel) Direct Coombs Test (EDTA Blood & Smear) D-Dimer (Citrated Blood) Serum protein electrophoresis

5 Histology Processing Price List Feb Charity No SC HISTOLOGY AND IMMUNOHISTOCHEMISTRY PROCESSING Block & Haematoxylin & Eosin Slide 8.00 Haematoxylin & Eosin slide Only 4.50 Special stain - Standard 9.00 Special stain Complex Immunohistochemistry 1: Antibody and kit provided by our laboratory Immunohistochemistry 2: Antibody supplied to us; Kit supplied by our laboratory Immunohistochemistry 3: Antibody and kit supplied to us Immunohistochemistry 4: New antibody optimisation Blank Paraffin Section 3.50 Section for Polymerase Chain Reaction 3.50 Process Only 4.50 Process & Blank Paraffin Section 6.50 Process & Special Stain We are continually updating our range of monoclonal antibodies. Please contact us to discuss your specific requirements We offer a specialised immunohistochemistry service for research including optimisation of custom antibodies Not all monoclonal antibodies will be available routinely due to variations in availability, demand and batch variation Veterinary Diagnostic Services, School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, United Kingdom Tel: +44 (0) Fax: +44 (0) vet-sch-vds@glasgow.ac.uk Website:

6 . Veterinary Diagnostic Services Pathology Price List Feb Charity No SC HISTOPATHOLOGY Biopsy 1-3 slides stained with haematoxylin and eosin 4-6 slides stained with haematoxylin and eosin Equine liver biopsy Canine liver biopsy Large biopsy specimen submissions (amputated leg, entire spleen, large hepatic or pulmonary lobectomy specimens, other). A pathologist will select and trim an adequate number of samples for histopathological assessment. 100 (up to 5 slides; additional slides as per price list) Additional slides (per slide) 8.00 Special stains: PAS, ZN, Gram (per stain) Standard stain/complex 9.00/ Note: Special stains may be included in biopsy price at the discretion of the veterinary pathologist H&E, Perls Prussian Blue, Masson`s Trichrome 52 H&E, Rhodanine, Massons Trichrome 52 IMMUNOHISTOCHEMISTRY (IHC) Tumour panels Including a report by a veterinary pathologist Lymphoma panel CD3, PAX Standard tumour panel Cytokeratin, Vimentin Round cell tumour panel Cytokeratin, Vimentin, CD3, PAX5, Astra blue (similar to toluidine blue for detection of mast cell granules) Extended round cell tumour Cytokeratin, Vimentin, CD3, PAX5, MHC-II, CD18, Astra blue panel Histiocytic tumour panel Vimentin, MHC-II, CD18, CD3, PAX5 (MAC387 and lysozyme may be added to panel at each) Melanoma panel Cytokeratin, Vimentin, Melan-A, PNL2, S Spindle cell tumour panel Vimentin, S-100, Desmin, Smooth muscle actin (CD117 [c-kit] is also available for gastrointestinal tumours) Neuronal panel Glial fibrillary acidic protein, Synaptophysin, S Vascular endothelial cell von Willebrand factor, CD31 (May be of value in diagnosis of markers haemangiosarcomas) Endocrine markers Insulin, Chromogranin A Ki67 proliferation index Ki67 (Usually used to determine Ki67 proliferation index of canine mast cell tumours) Other mast cell markers Mast cell tryptase, CD117 (c-kit) Other individual monoclonal antibodies and panels may be available on request, eg. Myeloperoxidase, HBME-1 (mesothelial cells) Additional antibodies included in the same report (additional cost per antibody) Canine CD Infectious disease markers Including a report by a veterinary pathologist Feline coronavirus May be of value in cases of suspected feline infectious peritonitis Feline herpesvirus May be of value in neonatal mortality in kittens with pneumonia Toxoplasma gondii Can be applied to a wide range of species Pneumocystis carinii Cross-reacts with canine Pneumocystis spp Helicobacter pylori Cross-reacts with canine and feline Helicobacter spp We are continually updating our range of monoclonal antibodies. Please contact us to discuss your specific requirements We offer a specialised immunohistochemistry service for research including optimisation of custom antibodies Not all monoclonal antibodies will be available routinely due to variations in availability, demand and batch variation Veterinary Diagnostic Services, School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, United Kingdom Tel: +44 (0) Fax: +44 (0) vet-sch-vds@glasgow.ac.uk Website:

7 Post-Mortem Service Prices Feb Charity No SC POST MORTEM EXAMINATION Each post mortem examination includes the gross post mortem examination and histopathology (up to 10 slides). Additional slides, special stains, and IHCs will be charged as above. Due to increasing prices of disposal, animals will be charged additionally for disposal (see under Disposal Charges). Dog 210 Neonatal death: pup/kitten 120 Cat 120 Horse 320 Cattle 250 Sheep, Goat and Pig 150 Camelid 150 Poultry 25 Rabbit 65 Exotic Species (reptiles) 120 Neurological cases (complete examination of brain and spinal cord) 150 Additional Legal and Insurance cases 160 Additional Additional court appearances and preparation 160/hour LARGE BIOPSY SPECIMEN SUBMISSIONS, ALL SPECIES See Histopathology/IHC Price List DISPOSAL CHARGES All animals received into the post mortem room will be charged for disposal whether a post-mortem examination is performed or not. Cremation Less than 1kg Charges levied by the cremation company Free 1kg to 5kg 30 6kg to 30kg 60 30kg to 100kg 85 Greater that 100kg 120 Laboratory rodents (mouse, rat, guinea pig, hamster, gerbil) cases of sudden unexpected death during experiments or outside experimental studies Routine diagnostic evaluation 60 Neurologic diagnostic evaluation 80 Veterinary Diagnostic Services, School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, United Kingdom Tel: +44 (0) Fax: +44 (0) vet-sch-vds@glasgow.ac.uk Website:

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