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1 1. - Meat and Meat Products Enterococcus Nordic Committee on Food Analysis, No.68, 5 th Edition (2011). - Egg and Egg Products) Total Bacterial Count FDA BAM Online, (Chapter 3) - Carcass - Animal skin Coagulase-positive Staphylococci and ISO : 1999 (Baird-Parker Agar Medium (BPK Technique)). - Animal Entrails Staphylococcus aureus ISO : 1999 (Rabbit-Plasma Fibrinogen - Dairy and Dairy products (RPF Technique)). - Dog Chew Clostridium perfringens FDA BAM Online, (Chapter 16). Salmonella spp. ISO 6579: 2002./Cor. 1: 2004 Enterobacteriaceae ISO 21528: 2004 Coliform and Escherichia coli FDA BAM Online, (Chapter 4). Listeria monocytogenes AOAC (2012) (BAX System; PCR Staphylococcal Enterotoxin Transia plate Staphylococcal Enterotoxin. Diffchamb (Sandwich-type ELISA 2. - Meat - Animal Entrails Detection of Anti-bacterial Substance Residues (Screening test) In-house method BQCLP_VPHL_FM_T5.4_06 (Agar Diffusion assay) in connection with: 1. Food Additives and Contaminants (2006) Vol.23 (1): Veterinary Sciences Division, Department of Agriculture and Rural Development for Northern Ireland. Belfast s Standard Operating Procedure. SOP CSD 222 V1 (2004), The Detection of Residues of Anti-bacterial Substances in Animal Tissues (Six Plate Method, Agar diffusion Test) 3. Water and Ice Coliforms Standard Methods for the Examination of Escherichia coli Water and Wastewater, APHA, AWWA, WEF. 22 nd Edition, Part Page 1 of 10 pages
2 4. Carcass Rinse Salmonella spp. USDA (2011) MLG4C.03 (BAX System; PCR 5. Animal fat Organochlorine Pesticides (OCPs ) - Hexachlorobenzene (HCB) - Hexachlorocyclohexane alpha isomer (HCB α-bhc) - Hexachlorocyclohexane beta isomer (HCB β-bhc) - Hexachlorocyclohexane gamma isomer (HCB γ-bhc) - Heptachlor - Heptachlor epoxide - Aldrin - Dieldrin - p,p -DDE - p,p -DDD - o,p -DDT - p,p -DDT - Chlordane cis isomer (α-chlordane) - Chlordane trans isomer (γ-chlordane) - Oxychlordane 6. - Muscle - Liver Total Arsenic Cadmium In-house Method BQCLP_VPHL_CR_ T5.4_01 Based on Journal Association Official Analytical Chemists (1984) Vol.67, No. 2, P (GC-ECD In-house Method BQCLP_VPHL_CR_ T5.4_02 Based on AOAC (2012), (AAS In-house Method BQCLP_VPHL_CR_ T5.4_03 Based on AOAC (2012), (AAS 7. Honey Total Arsenic In-house Method BQCLP_VPHL_CR_ T5.4_04 Based on AOAC (2012), (AAS Cadmium In-house Method BQCLP_VPHL_CR_T5.4_05 Based on AOAC (2012) (AAS Technique) Page 2 of 10 pages
3 8. Muscle Liver Lead Honey Total Mercury 9. Muscle Sulfonamides - Sulfadiazine - Sulfapyridine - Sulfamerazine - Sulfamethazine - Sulfamonomethoxine - Sulfachloropyridazine - Sulfamethoxazole - Sulfadimethoxine - Sulfaquinoxaline Animal Tissue and Processed Products - Egg Protein bound Nitrofurans Metabolites; ) - Nitrofurantoin (Metabolite: 1- aminohydantoin, AHD) - Furazolidone (Metabolite: 3- amino- 2- oxazolidinone, AOZ) - Furaltadone (Metabolite: 3-amino-5- morpholinomethyl-1, 3- oxazolidin-2-one, AMOZ) - Nitrofurazone (Metabolite: semicarbazide, SEM) In-house Method BQCLP_VPHL_CR_ T5.4_08 Based on AOAC (2012), (AAS In-house Method BQCLP_VPHL_CR_ T5.4_09 Based on EPA Method (2007) Rev. 0, Mercury in Solids and Solutions by Thermal Decomposition, Amalgamation and Atomic Absorption Spectrophotometry. P (Direct Mercury Analyzer Technique) In-house method BQCLP_VPHL_DR_ T5.4_01 Based on Western Australian Regional Laboratory of Australian Government Analytical Laboratories (AGAL) Method, No. 7, Issued June (High Performance Liquid Chromatography (HPLC) UV Detector In-house method BQCLP_VPHL_DR_ T5.4_02 (LC-MS/MS Technique) based on 1. J. of Chromatography A., (2001), Vol P Veterinary Sciences Division, Department of Agriculture and Rural Development for Northern Ireland, Belfast s Standard Operating Procedure. Determination of Protein bound Nitrofuran Residues in Chicken Tissues Using LC-MS-MS. 21 May Page 3 of 10 pages
4 11. - Animal Tissue and Processed Products - Egg Chloramphenicol In-house Method BQCLP_VPHL_DR_ T5.4_03 (LC-MS/MS Technique) based on 1. Analytical Chemica Acta. 2003, Vol. - Milk 483, P Honey 2. Food Additives and Contaminants. 12. Urine Beta - agonists - Brombuterol - Cimaterol - Cimbuterol - Clenbuterol - Hydroxymethylclenbuterol - Mabuterol - Mapenterol - Ractopamine - Salbutamol - Zilpaterol 2003, Vol. 20, No. 4, P In-house Method BQCLP_VPHL_DR_ T5.4_04 (LC-MS/MS Technique) based on 1. BVL, EU and National Reference Laboratory for Residues Short Description Method BETA_014. V. 1. (10 June 2005). Determination of Betaagonist in Urine Using Screening dau Cartridges with LC-MS/MS. 2. Commission Decision 2002/657 of 12 August Off. J. Eur. Commun. L221/ Commission Regulation (EU). No. 4. Corley, J Handbook of Residue Analytical Methods for Agrochemicals. P Page 4 of 10 pages
5 13. Muscle Beta - agonists - Brombuterol - Cimaterol - Cimbuterol - Clenbuterol - Hydroxymethylclenbuterol - Mabuterol - Mapenterol - Ractopamine - Salbutamol - Zilpatero In-house Method BQCLP_VPHL_DR_ T5.4_05 (LC-MS/MS Technique) based on 1. BVL, EU and National Reference Laboratory for Residues Short Description Method BETA_012. Determination of Beta-agonist in Muscle Using Screening dau Cartridges with LC-MS/MS. 2. Commission Decision 2002/657 of 12 August Off. J. Eur. Commun. L221/ Commission Regulation (EU). No. 4. Corley, J Handbook of Residue Analytical Methods for Agrochemicals. 14. Liver Beta - agonists - Brombuterol - Cimaterol - Cimbuterol - Clenbuterol - Hydroxymethylclenbuterol - Mabuterol - Mapenterol - Ractopamine - Salbutamol - Zilpatero P In-house method BQCLP_VPHL_DR_ T5.4_06 (LC-MS/MS Technique) based on 1. BVL, EU and National Reference Laboratory for Residues Short Description Method BETA_012. Determination of Beta-agonist in Muscle Using Screening dau Cartridges with LC-MS/MS. 2. Commission Decision 2002/657 of 12 August Off. J. Eur. Commun. L221/ Commission Regulation (EU). No. 4. Corley, J Handbook of Residue Analytical Methods for Agrochemicals. P Page 5 of 10 pages
6 15. Muscle Tetracyclines - Oxytetracycline - Tetracycline - Chlortetracycline - Doxycycline In-house Method BQCLP_VPHL_DR_ T5.4_09 (LC-MS/MS Technique) based on 1. Hurtaud-Pessel, et. al Euro Residue VI: Conference on Residue of Veterinary Drugs in Food. P Guidelines for the Design and Implementation of National Regulatory Food Safety Assurance Programme Associated with the Use of Veterinary Drugs in Food Producing Animals. CAC/GL Corley, J Handbook of Residue Analytical Methods for Agrochemicals. P Commission Decision 2002/657 of 12 August Off. J. Eur. Commun. L221/ Commission Regulation (EU). No. Page 6 of 10 pages
7 16. Egg Fluoroquinolone - Enrofloxacin - Ciprofloxacin In-house Method BQCLP_VPHL_DR_ T5.4_07 (LC-MS/MS Technique) based on 1. Hurtaud-Pessel, et. al Euro Residue VI: Conference on Residue of Veterinary Drugs in Food. P Durden and MacPherson J. of AOAC International. Vol. 90, No. 2, P Guidelines for the Design and Implementation of National Regulatory Food Safety Assurance Programme Associated with the Use of Veterinary Drugs in Food Producing Animals. CAC/GL Corley, J Handbook of Residue Analytical Methods for Agrochemicals. P Commission Regulation (EU). No. Page 7 of 10 pages
8 17. Muscle Fluoroquinolone - Enrofloxacin - Ciprofloxacin In-house Method BQCLP_VPHL_DR_ T5.4_08 (LC-MS/MS Technique) based on 1. Hurtaud-Pessel, et. al Euro Residue VI: Conference on Residue of Veterinary Drugs in Food. P Guidelines for the Design and Implementation of National Regulatory Food Safety Assurance Programme Associated with the Use of Veterinary Drugs in Food Producing Animals. CAC/GL Corley, J Handbook of Residue Analytical Methods for Agrochemicals. P Commission Decision 2002/657 of 12 August Off. J. Eur. Commun. L221/ Commission Regulation (EU). No. 18. Veterinary Drugs (Sterile ph Value USP 37/ NF 32: 2014 and BP Injectable Solution) 19. Tylosin Injectable Tylosin British pharmacopoeia (Veterinary) 2014 Solution (Microbiological assay method). 20. Enrofloxacin Injection Enrofloxacin In-house method BQCLP_VDAL_ T5.4_02 based on USP-37/ NF 32: Monograph Enrofloxacin; Related compounds. P (High Performance Liquid Chromatography (HPLC) Diode Array Detector Technique.) 21. Disinfectants Efficacy of Disinfectants - Staphylococcus aureus - Salmonella enterica - Pseudomonas aeruginosa AOAC Official Method of Analysis. Chapter 6. by Use Dilution Method. - Staphylococcus aureus (955.14, 2013) - Salmonella enterica (955.15, 2013) - Pseudomonas aeruginosa (964.02, 2012). Page 8 of 10 pages
9 22. Potable Water from Livestock Farms Beta agonists - Salbutamol - Clenbuterol HCl - Ractopamine HCl In-house Method BQCLP_VDAL_ T5.4_09 (LC/MS-Ion Trap Technique) in Connection with: 1. Analytica Chimica Acta 529 (2005): Commission Decision 2002/657/EC. Implementing Council Directive 96/23/EC. Concerning the Performance of Analytical Methods and the Interpretation of Results. 2002; Funk W, Dammann V, Donnevert G. Quality Assurance in Analytical chemistry, 2 nd ed ; 2007 : Irish national accreditation board; Wilton Park House, Wilton Place, Dublin 2, Ireland. PS15 Guide to method validation for quantitative analysis in chemical testing laboratories Issue; ISO : Capability of Detection Part 2: Methodology in the linear calibration case. 6. ISO : Capability of Detection Part 5: Methodology in the linear and nonlinear calibration case. 7. Miller,NJ, Miller,CJ. Statistics and Chemome-trics for Analytical chemistry. 5 th ed. London: British Library; SANCO/ 2004/2726-rev; 4-December Guidelines for the Implementation of Decision 2002/657/EC. Page 9 of 10 pages
10 23 Milk (Raw milk, milk Aerobic plate count FDA BAM Online, (Chapter 3) products) Coliform FDA BAM Online, (Chapter 4) 24 Muscle Nitroimidazoles - Dimetridazole (DMZ) - 2-hydroxymethyl-1- methyl-5-nitroimidazole (HMMNI) - Ipronidazole (IPZ) - Hydroxyipronidazol (IPZOH) - Metronidazole (MNZ) - Hydroxymetronidazole (MNZOH) - Ronidazole (RNZ) 25 Egg and Honey Total Residue Nitrofuran Metabolites; 26 Meat and Meat Products - Egg and Egg Products - Carcass - Animal skin - Animal Entrails - Dairy and Dairy products - Dog Chew - 3-amino-5- morpholinomethyl-1,3- oxazolidin-2-one (AMOZ) - 3-amino-2-oxazoilidinone (AOZ) - 1-aminohydantoin (AHD) - semicarbazide (SEM) Coagulation positive Staphylococci and Staphylococcus aureus In-house method BQLCP_VPHL_DR_T5.4_11 by LC-MS/MS based on BVL, EU and national Reference lab, Nitroimidazoles in muscle and plasma by LC-MS/MS short description ( ) NIM_009 In-house method BQLCP_VPHL_DR_T5.4_12 by LC-MS/MS based on Leitner, A., Zollner, P., Linder, W., 2001, Determination of the metabolites of nitrofuran antibiotics in animal tissue by high-performance liquid chromatography-tandem mass spectrometry. J.Chromatography A, 939, ISO :2003 (Detection and MPN technique for low numbers) Page 10 of 10 pages
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