Drug Residue Screening
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- Barnaby Hunt
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1 FOOD DIAGNOSTICS Drug Residue Screening Reduce labour, increase productivity and decrease costs
2 Drug residue testing With nearly 30 years experience in the market and a dedicated research and development team, Randox Food Diagnostics is committed to the development and manufacturing of high quality and accurate drug residue testing solutions. This unique multi-analyte testing platform will reduce labour costs, increase throughput and guarantee overall productivity improvement. Drug residues are causing concern increasingly worldwide. Most drugs are introduced to improve animal health however, the abuse of drugs is becoming increasingly widespread. The legitimate treatment of disease with drugs such as antibiotics (in clinical doses) reduces mortality in livestock while other growth promoting agents are mis-used to improve meat production. Reduce labour costs, increase throughput and guarantee overall productivity improvement Global racing authorities are reliant upon drug residue screening solutions to maintain the integrity of the racing industry by testing for illegal performance altering drugs. An increasing number of compounds are being screened to protect the safety of animals and to ensure races are won on the animals natural ability. As a consequence of the abuse of drugs within the food production and animal racing industries, the practice of using high doses of antibiotics, growth promoters and drugs of abuse is strictly regulated. Randox Food Diagnostics Products ELISA kit - For screening animal and food samples for drug residues - For monitoring show and competition animals for performance enhancing drugs - Applicable to multiple matrices Immunoaffinity columns - For sample purification prior to analyte assessment - To complement Randox Food Diagnostics ELISA and Biochip Array Technology - Compatible with LCMS / HPLC / GCMS Multiple residue screening - For multi-analyte screening of a comprehensive range of drug residues - Considerable time, labour and cost savings - Higher throughput - Applicable to multiple matrices
3 ELISA Tests for Drug Residue Analysis Randox Food Diagnostics ELISA kits allow screening of drug residues in various matrices. Randox Food Diagnostics ELISA kits show favourable comparison with confirmatory methods. Randox Food Diagnostics ELISA Kit Benefits Easy to use, highly specific, cost effective screening method Measurement of drug residues in various matrices in urine, serum/plasma, tissue, muscle, feed, milk, honey, feather, bile, hair, egg and retina Intra and inter assay precision typically <10% CV Fast up to 80 samples can be analysed in two to three hours with ELISA Lower set-up costs as no expensive equipment required Randox ELISA tests can be fully automated Drug residue ELISA kits available Drug class Cat. No. Drug residue Anabolic steroids Anti-microbial Other veterinary drugs b-agonists TB2106 NT2105 BD2382 SW2418 SQ2145 SZ2147 SM2146 SZ3471 CN1469 BL3448 BL1371 QL3454 FQ3460 NF3465 NF3462 NF3463 NF3461 AV3477 EC3473 STP3468 Available 2013 RLS9225 RLS9222 CB1418 RT3451 SU2148 Trenbolone 19-Nortestosterone/Trenbolone Boldenone Stanozolol Sulphaquinoxaline Sulphadiazine Sulphamethazine Sulphamethoxazole Chloramphenicol b-lactams b-lactams standard accessory kit Quinolones Flumequine AOZ AMOZ AHD SEM Avermectin ELISA Enro/Ciprofloxacin ELISA Streptomycin/Dihydrostreptomycin Tetracyclines (generic) Ethinylestradiol Bacitricin Drugs of abuse KT3459 Ketamine (specific for norketamine) Clenbuterol/brombuterol Ractopamine b-agonists (clenbuterol, salbutamol, mabuterol, brombuterol, mapenterol, terbutaline, pirbuterol, methylclenbuterol, carbuterol, mabuterol, bromochlorbuterol, cimbuterol, clenpenterol hydrochloride) Tri-phenylmethane dyes LMG3466 Leucomalachite Green (Leucomalachite Green, Malachite Green, Leucocrystal Violet) Non steroidal Anti-inflammatory Oestrogenic DM2156 PB3456 FL3458 SJ2152 ZR2421 Urine controls CB3449 Clenbuterol Corticosteroids (dexamethasone, flumethasone, betamethasone) Phenylbutazone Flunixin Stilbenes (diethylstilbestrol, hexestrol, dienestrol) Zeranol Easy to use and cost effective ELISA screening solutions
4 Immunoaffinity columns for sample purification Sample clean up is an essential part of drug residue testing, whether it is performed by ELISA, LC-MS or any other technique. To complement this range of ELISAs for measuring drug residues, Randox Food Diagnostics offers a wide range of Immunoaffinity Columns for sample purification. Randox Food Diagnostics Immunoaffinity column benefits Very efficient and highly specific Multi-analyte testing allowing simultaneous sample clean up Higher analyte recovery Improved safety and reduced waste and disposal costs Faster due to fewer purification steps Can be automated Convenient available in packs of five with buffers for equilibration and storage Cost-effective each column can be used up to 10 times Long shelf life two years from date of manufacture The principle of sample purification by immunoaffinity columns Addition of crude sample that runs through column gravimetrically Biochip Y Y Y Y Y Y ELISA Measurement of Eluent Sample Pretreatment 1 cm Elution of bound analyte HPLC/LCMS Immunoaffinity columns available Cat No. Product Description Pack Size GP1821 Growth Promoters (Boldenone, Zeranol, Ractopamine, Corticosteroids, Nandrolone, Stilbenes, Trenbolone, Stanozold, Beta-Agonist) 5 columns Y X Y Y X Y Y Y X X Antibody Antigen Interferrant Interferants removal by column washing Y Y Y Y Y Y CB2184 DM2185 Clenbuterol/ß-agonists (Clenbuterol, salbutamol, mabuterol, mapenterol, terbutaline, cimaterol, perbuterol, carbuterol, brombuterol, methylclenbuterol) Corticosteroids (Dexamethasone, betamethasone, flumethasone) 5 columns 5 columns TB2186 Trenbolone and 19-nortestosterone 5 columns Analytes of interest bind to immobilised antibodies in the IAC gel SJ2154 Stilbenes (Diethylstilbestrol, hexestrol, dienestrol) 5 columns ZR2420 Zeranol 5 columns Cost effective immunoaffinity columns
5 Biochip Array Technology for Multi-Analyte Testing Randox Food Diagnostics presents the world s only multi-analyte quantitative drug residue analyser. The use of the Evidence Investigator allows simultaneous detection of multiple analytes from a single sample, using our patented Biochip Array Technology. This unique platform consolidates testing, reduces labour costs and improves overall productivity. Biochip Array Technology benefits Cost savings and minimal consumable costs Less labour (Minimal labour costs could be reduced by at least five fold) Higher throughput (45 samples or 675 tests in under two hours) Faster adaptability to new regulations (up to 65 new antibodies at the R+D stage) Faster turnaround time Applicable to multiple matrices Honey Feed Milk Urine Egg Tissue Ideal for drug screening laboratories Multiplex screening for veterinary drug residues Consolidation of testing to provide multiple test results from a single sample Reduces labour costs and improves overall productivity Requires minimal technical expertise Extensive menu of drug residue tests developed Same sample preparation method can be applied across a number of arrays. Sensitivities meet both US safe levels and EU maximum residue levels Multi-analyte screening for veterinary drug residues
6 Randox Food Diagnostics veterinary drug residue test menu ANTIMICROBIAL ARRAYS Anthelmintics Array (EV3770) Amino Benzimidazoles Avermectins (generic) Benzimidazoles Levamisole Moxidectin Thiabendazole Triclabendazole Antimicrobial Array I plus (EV3775) Sulphachlorpyridazine Sulphadiazine Sulphadimethoxine Sulphadoxine Sulphamerazine Sulphamethazine Sulphamethizole Sulphamethoxazole Sulphamethoxypyridazine Sulphamonomethoxine Sulphapyridine Sulphaquinoxaline Sulphathiazole Sulphisoxazole Trimethoprim Antimicrobial Array II (EV3524) Ceftiofur Florphenicol/ Thiamphenicol Quinolones (generic) Streptomycin/ Dihydrostreptomycin Tetracyclines (generic) Tylosin/ Tilmicosin Antimicrobial Array III (EV3695) AHD AMOZ AOZ SEM Chloramphenicol Chloramphenicol Glucuronide Antimicrobial Array III Chloramphenicol only (EV3738) Chloramphenicol Chloramphenicol Glucuronide Antimicrobial Array AMIV Lincomycin / Clindamycin Erythromycin Gentamicin Neomycin / Paromomycin Apramycin Streptomycin / Dihydrostreptomycin Amikacin / Kanamycin Bacitracin Spectinomycin Spiramycin / Josamycin Desmycosin (Tylosin B) Tylosin AVERMECTINS ONLY ARRAY (EV3842) Avermectins (generic) GROWTH PROMOTERS ARRAYS Growth Promoter Multiple Matrix Screen (EV3726) b - Agonists Boldenone Corticosteroids Nandrolone Ractopamine Stanozolol Stilbenes Trenbolone Zeranol β - LactamS Array (EV3793) Penicillins Amoxicillin Ampicillin Cloxacillin Dicloxacillin Nafcillin Oxacillin Penethamate Penicillin G Penicillin V Growth Promoter Rapid Screen (EV3521) b - Agonists Boldenone Corticosteroids Ractopamine Stanozolol Trenbolone Zeranol Synthetic Steroids Array (EV3694) 17b - Clostebol Ethinylestradiol Gestagens (generic) Methyltestosterone Cephalosporins Cefacetril Cefazolin Cefoperazone Cefquinome Ceftiofur Cefuroxime Cephalexin Cephalonium Cephapirin Multi-analyte screening, the ultimate cost saving drug residue screening solution
7 Publications Examples using Randox Food Diagnostics testing products Sterk S.S., Ohlrich D., Christensen, F., Le, N.B., Blokland, M.H., Schwillens, P.L.W.J., van Ginkel, L.A., and Stephany, R.W. Screening and confirmation of real-life urine samples for trenbolone-false positive or false negative? That s the question! Proceedings EuroResidue IV, 2000, Stenhouse, A.M., Keledjian J., Vadasz, A., Dunstan, A.J., Suann, C.J., McKinney, A.R. Feed contamination leading to the detection of ractopamine in post race urine samples. Proceedings of the 16 th International Conference of Racing Analysts and Veterinarians, 2006, Ponniah, J., Muhammad K., Abdullah, S., Ganapathy, K.K., bt Sheikh Abdul Hamid, N. Validation of ELISA test kits for detection of beta-agonist residues in meat. Southeast Asian J. Trop. Med. Public Health, 2004, 35(2): FitzGerald, S.P., O Loan, N., McConnell, R.I., Benchikh, E.O., Kane, N.E. Stable Competitive Enzyme-Linked Immunosorbent Assay Kit for Rapid Measurement of 11 Active Beta-Lactams in Milk, Tissue, Urine and Serum. Journal of AOAC International. 2007, 90(1): Example Randox Poster Presentations (ELISA) Lowry A.P., Benchikh E.O., O Loan N., Fitzgerald S.P., McConnell R.I., Bryson D. Development of a generic direct competitive ELISA for the rapid screening of quinolones in raw milk and food animal edible tissue Fifth International Symposium on Hormone and Veterinary Drug Residue Analysis, Antwerp Belgium, 2006 Benchikh E.O., Lowry A.P., FitzGerald S.P., McConnell R.I., Kane E.N., Bryson D. Development of a competitive ELISA for the detection of malachite green and leucomalachite green. Fifth International Symposium on Hormone and Veterinary Drug Residue Analysis, Antwerp Belgium, 2006 Example Randox Poster presentations (Biochip) Tohill A., McAleer D., McConnell R.I., FitzGerald S.P. Evidence Biochip Array Technology as a multi-analytical tool for the screening of residues in different food matrices. RAFA, 2009 Tohill A., McAleer D., Acheson R., Bell B., McConnell R.I., FitzGerald S.P. Application of Biochip Array Technology to multi-analyte screening of sulphonamides in different matrices. EuroResidue VI, Netherlands, 2008 O Mahony, et al. Simultaneous detection of four nitrofuran metabolites in honey using multiplexing biochip screening assay. Biosens Bioelectron. 2011, 26(10): J. Porter, P. Lowry, R. I. McConnell, A. Tohill, S. P. Fitzgerald Anthelmintics Evidence Investigator, 6th International Symposium on hormone and veterinary drug residue analysis Ghent- Belgium, M. Clarke, J Porter, E.O Benchikh, R.I McCconnell, S.P Fitzgerald AM I Plus, AM II, AM III in different food matrices AOAC 25th Annual meeting, USA, D Ferguson, J Porter, J Mahoney, R.I McConnell, S.P Fitzgerald Quantitative generic screening of 16 Beta-Lactam antibiotic residues in milk samples on a biochip platform. J. Porter, P. Lowry, R. I. McConnell, A. Tohill, S. P. Fitzgerald Multiplex screening with Evidence biochip arrays: applications to the detection of anthelmintic, sulphonamides & coccidiostat drug residue Ferguson D., Porter J., Farry L., McConnell R.I., Fitzgerald S.P. Development of highly sensitive enzyme-linked immunosorbent assay (ELISA) for the screening of Bacitracin. Euroresidue, The Netherlands, 2012 Ferguson D., Porter J., Farry L., McConnell R.I., Fitzgerald S.P. Development of a fast generic milk preparation method for application across five enzyme-linked immunosorbent (ELISA) kits. Euroresidue, The Netherlands, 2012 FitzGerald, S.P., O Loan, N., McConnell, R.I., Benchikh, E.O., Kane, N.E. Stable Competitive Enzyme-Linked Immunosorbent Assay Kit for Rapid Measurement of 11 Active Beta-Lactams in Milk, Tissue, Urine and Serum. Journal of AOAC International. 2007, 90(1): Ponniah, J., Muhammad K., Abdullah, S., Ganapathy, K.K., bt Sheikh Abdul Hamid, N. Validation of ELISA test kits for detection of beta-agonist residues in meat. Southeast Asian J. Trop. Med. Public Health, 2004, 35(2): For further information visit
8 Fast track your workload Screening test menu includes Sulphonamides Chloramphenicol Tetracyclines Nitrofurans Aminoglycosides Macrolides Quinolones Beta Lactam Anthelmintics Gestagens/Androgens/ Estrogens Corticosteroids Beta Agonist Clenbuterol Ractopamine Boldenone Stanozolol FOOD DIAGNOSTICS Randox Food Diagnostics provides a unique multi-analyte testing platform that will improve the speed of throughput of your food testing laboratory. Multi-analyte testing benefits: Fast sample turnaround time Reduce labour costs Overall productivity improvements Consolidates testing Simple sample preparation Quality reassurance Trenbolone Nandrolone Zeranol Matrices available: Tissue Honey Feed Urine Milk Egg Randox Food Diagnostics, 30 Cherryvalley Road, Crumlin, County Antrim, BT29 4QN, United Kingdom t +44 (0) f +44 (0) e enquiries@randoxfooddiagnostics.com I Information correct at time of print. All Randox Food Diagnostics products are made in the UK. Randox Food Diagnostics Limited is a company registered within Northern Ireland with Company Number NI VAT Number GB Product availability may vary from country to country. Please contact your local Randox Food Diagnostics representative for information. LT015RFD NOV12
Drug Residue Screening
FOOD DIAGNOSTICS Drug Residue Screening Reduce labour, increase productivity and decrease costs Drug Residue Testing With nearly 30 years experience in the market and a dedicated research and development
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