1. African Savannah Elephant 6. Gray, Short-tailed Opossum (Loxodonta africana) (Monodelphis domestica)

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1 1. African Savannah Elephant 6. Gray, Short-tailed Opossum (Loxodonta africana) (Monodelphis domestica) 2. Chimpanzee 7. Horse (Pan troglodytes) (Equus caballus) 8. Mouse 3. Common Shrew (Mus musculus) (Sorex araneus) 4. Dog 9. Nine-banded Armadillo (Canis lupus) (Dasypus novemcinctus) 5. European Hedgehog 10. Tenrec (Erinaceus europaeus) (Echinops telfairi)

2 Bacteria Archaea Eukaryotes Time

3

4 Two ways to make phylogenetic trees: 1. Using physical characteristics 2. Using DNA sequence

5 Two ways to make phylogenetic trees:! 1. Using physical characteristics! 2. Using DNA sequence!

6 Two ways to make phylogenetic trees:! 1. Using physical characteristics!

7 Two ways to make phylogenetic trees:! 1. Using physical characteristics! rat! mouse! frog!

8 1. African Savannah Elephant 6. Gray, Short-tailed Opossum (Loxodonta africana) (Monodelphis domestica) 2. Chimpanzee 7. Horse (Pan troglodytes) (Equus caballus) 8. Mouse 3. Common Shrew (Mus musculus) (Sorex araneus) 4. Dog 9. Nine-banded Armadillo (Canis lupus) (Dasypus novemcinctus) 5. European Hedgehog 10. Tenrec (Erinaceus europaeus) (Echinops telfairi)

9 platypus! cow! macaque!

10 Two ways to make phylogenetic trees: 1. Using physical characteristics 2. Using DNA sequence

11 How to write a DNA sequence 5 3 A T T A G C C G C G G C A T T A C G G C T A T A ATGCCGATCGTT-3 3 -TACGGCTAGCAA-5 5 -ATGCCGATCGTT-3

12 Two ways to make phylogenetic trees: 1. Using physical characteristics 2. Using DNA sequence 5 -GATGACGTACG-3 5 -CAACATGGAAG-3 5 -GATTACGTACG-3

13 Two ways to make phylogenetic trees: 2. Using DNA sequence 5 -GATGACGTACG-3 5 -CAACATGGAAG-3 5 -GATTACGTACG-3 first second third

14 Two ways to make phylogenetic trees: 2. Using DNA sequence 5 -GATGACGTACG-3 5 -CAACATGGAAG-3 5 -GATTACGTACG-3 first second third

15 Two ways to make phylogenetic trees: 2. Using DNA sequence GATGACGTACG-3 first 5 -CAACATGGAAG-3 second 5 -GATTACGTACG-3 third 6

16 Two ways to make phylogenetic trees: third first 2. Using DNA sequence second GATGACGTACG-3 first 5 -CAACATGGAAG-3 second 5 -GATTACGTACG-3 third 6

17 Cow: 5 -ACCGTATTTGCCGAAG-3 Dog: 5 -AGCGTAATTGCCGTAG-3 Hedgehog: 5 -AGCGTAGTTTCCGTAC-3 Horse: 5 -AGCGTACTTGCCGTAG-3 Mouse: 5 -ATAGTAGATCGCGCAT-3 Shrew: 5 -AGCGTAGTTACCGTAC-3

18 Cow Dog Hedgehog Horse Mouse Shrew Cow Dog Hedgehog 3 Horse Mouse Shrew

19 Cow Dog Hedgehog Horse Mouse Shrew Cow Dog Hedgehog 3 Horse Mouse Shrew

20 platypus opposum tenrec elephant armadillo cow macaque chimp

21 platypus opposum tenrec elephant armadillo cow Cow Dog Hedgehog Horse Mouse Shrew Cow Dog 3 5 Hedgehog 3 macaque Horse Mouse Shrew chimp

22 platypus opposum tenrec elephant armadillo hedgehog shrew horse dog cow mouse Cow Dog Hedgehog Horse Mouse Shrew macaque chimp Cow Dog Hedgehog 3 Horse Mouse Shrew

23 Do everyone s trees based on physical characteristics look the same? Why or why not? Do everyone s DNA-based trees look the same? Why or why not?

24 Which method do you think is used to generate current, scientifically accepted phylogenetic trees?

25 ATGCCGATCGTACGACACATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCATCGTACTGACTGCATCGATC TACTGACTGCATCGTACTGACTGCACATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCACATATCGTTTA CATCGTACTGACTGTCTAGTCTAAACACATCCCACATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCAGC CATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCTATGCCGATCGTACGACACATATCGTCATCGTACTGCC ACTGTCTAGTCTAAACACATCCATCGTACTGACTGCATCGTACTGACTGCATCGTACTGACTGCACATATCGTCATAC TCGTACTGACTGTCTAGTCTAAACACATCCCACATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCACTTT ATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCACATATCGTCATCGTACTGACTGTCTAGTCTAAACACA GCCGATCGTACGACACATATCGTCATCGTACTGCCCTACGGGACTGTCTAGTCTAAACACATCCATCGTACTGACTGC TGACTGCATCGTACTGACTGCACATATCGTCATACATAGACTTCGTACTGACTGTCTAGTCTAAACACATCCCACATA CGTACTGACTGTCTAGTCTAAACACATCCCACTTTACCCATGCATCGTACTGACTGTCTAGTCTAAACACATCCCACA ATCGTACTGACTGTCTAGTCTAAACACATCCCAGCATCCATCCATATCGTCATCGTACTGACTGTCTAGTCTAAACAC GCCGATCGTACGACACATATCGTCATCGTACTGCCCTACGGGACTGTCTAGTCTAAACACATCCATCGTACTGACTGC TGACTGCATCGTACTGACTGCACATATCGTCATACATAGACTTCGTACTGACTGTCTAGTCTAAACACATCCCACATA CGTACTGACTGTCTAGTCTAAACACATCCCACTTTACCCATGATATCGTCATCGTACTGACTGTCTAGTCTAAACACA TATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCTATACATATCGTCATCGTACTGACTGTCTAGTCTAAACAC GCCGATCGTACGACACATATCGTCATCGTACTGCCCTACGGGACTGTCTAGTCTAAACACATCCATCGTACTGACTGC TGACTGCATCGTACTGACTGCACATATCGTCATACATAGACTTCGTACTGACTGTCTAGTCTAAACACATCCCACATA CGTACTGACTGTCTAGTCTAAACACATCCCACTTTACCCATGATATCGTCATCGTACTGACTGTCTAGTCTAAACACA TATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCTATAGCCGATCGTACGACACATATCGTCATCGTACTGCCC CTGTCTAGTCTAAACACATCCATCGTACTGACTGCATCGTACGCCGATCGTACGACACATATCGTCATCGTACTGCCC CTGTCTAGTCTAAACACATCCATCGTACTGACTGCATCGTACTGACTGCATCGTACTGACTGCACATATCGTCATACA CGTACTGACTGTCTAGTCTAAACACATCCCACATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCACTTTA ATCGTACTGACTGTCTAGTCTAAACACATCCCACATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCAGCA ATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCTATGCCGATCGTACGACACATATCGTCATCGTACTGCCC CTGTCTAGTCTAAACACATCCATCGTACTGACTGCATCGTACGACTGCATCGTACTGACTGCACATATCGTCATACAT GTACTGACTGTCTAGTCTAAACACATCCCACATATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCACTTTAC ATCGTCATCGTACTGACTGTCTAGTCTAAACACATCCCACACTGTCTAGTCTAAACACATCCATCGTACTGACTGCAT CGATCGTACGACACATATCGTCATCGTACTGCCCTACGGGACTGTCTAGTCTAAACACATCCATCGTACTGACTGCAT

26 Which animal s placement on the DNA-based tree surprised you the most, and why?

27 1. African Savannah Elephant 6. Gray, Short-tailed Opossum (Loxodonta africana) (Monodelphis domestica) 2. Chimpanzee 7. Horse (Pan troglodytes) (Equus caballus) 8. Mouse 3. Common Shrew (Mus musculus) (Sorex araneus) 4. Dog 9. Nine-banded Armadillo (Canis lupus) (Dasypus novemcinctus) 5. European Hedgehog 10. Tenrec (Erinaceus europaeus) (Echinops telfairi)

28 platypus opposum tenrec elephant armadillo hedgehog shrew horse dog cow mouse macaque chimp

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