Tung-Yi Huang et al. Correspondence to: Cheng-Wei Fan

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1 Supplement of Biogeosciences, 15, , 21 Author(s) 21. This work is distributed under the Creative Commons Attribution 3. License. Supplement of Plant n-alkane production from litterfall altered the diversity and community structure of alkane degrading bacteria in litter layer in lowland subtropical rainforest in Taiwan Tung-Yi Huang et al. Correspondence to: Cheng-Wei Fan The copyright of individual parts of the supplement might differ from the CC BY 3. License.

2 Table S1. The taxonomy prediction of alkb lineage-like OTUs and the reference genes. Informatics prediction by UTAX algorithm gives the prediction up to genus level. An estimated probability that correct prediction was higher than 9% is showed on this table. Phylum OTU Taxonomy Prediction Relative Abundance (%) ravine windward leeward Reference of bacteria with alkb gene Sequence ID Identities litter14 g: Rhizobium ( 97%) Agrobacterium fabrum NR /32(%) litter44 f: Rhizobiaceae (%) Rhizobium etli U /31(%) litter5 o: Rhizobiales (9%) Ensifer adhaerens CP /32(9%) litter117 o: Rhizobiales (97%) 1.6 <.1 <.1 Mesorhizobium amorphae NR /34(97%) litter6 f: Bradyrhizobiaceae (93%) Bradyrhizobium diazoefficiens AB /32(%) litter159 f: Pseudomonadaceae (%).6 <.1 Stenotrophomonas maltophilia AM /46(%) litter464 o: Rhizobiales (%).4 <.1 <.1 Sinorhizobium meliloti CP /34(9%) litter19 c: Gammaproteobacteria (%).4 <.1 Stenotrophomonas maltophilia AM /4(97%) litter2 g: Pseudorhodoferax (97%).3 Delftia acidovorans CP4.1 37/4(95%) litter1 g: Caulobacter (95%).3.1 <.1 Caulobacter crescentus AE /34(%) litter2 o: Rhizobiales (9%).3 <.1.1 Bradyrhizobium diazoefficiens AB /37(97%) litter f: Phyllobacteriaceae (93%).2 <.1 Mesorhizobium ciceri CP /32(9%) Proteobacteria litter152 f: Caulobacteraceae (%).1.1 <.1 Caulobacter crescentus AE /34(%) litter19 o: Rhizobiales (95%).1 <.1.7 Ochrobactrum anthropi CP /32(9%) litter9 c: Alphaproteobacteria (%) Ochrobactrum anthropi CP /32(97%) litter21 f: Enterobacteriaceae (9%).1.7 <.1 Enterobacter lignolyticus CP /47(9%) litter55 g: Pseudomonas (95%) <.1 <.1 <.1 Pseudomonas putida LT /4(97%) litter47 f: Burkholderiaceae (94%) < Burkholderia pseudomallei CP /413(97%) litter75 f: Burkholderiaceae (95%) < Burkholderia phenoliruptrix CP /49(9%) litter11 f: Burkholderiaceae (95%) < Burkholderia phenoliruptrix CP /49(9%) litter34 f: Oxalobacteraceae (%) <.1 <.1 Herbaspirillum seropedicae CP /46(95%) litter29 f: Burkholderiaceae (%) < Burkholderia phenoliruptrix CP /41(97%) litter2 g: Ralstonia (%) <.1 Ralstonia solanacearum AL /46(97%) litter22 f: Burkholderiaceae (95%).1.2 Burkholderia pseudomallei CP /47(%) litter32 f: Pseudonocardiaceae (97%) 4. <.1 Amycolatopsis orientalis NR /39(%) litter69 g: Pseudonocardia (9%) 1.6 <.1 Amycolatopsis orientalis NR /3(95%) litter426 g: Streptomyces (%).5 <.1 <.1 Streptomyces virginiae NR /36(%) litter147 g: Pseudonocardia (%).4 <.1 <.1 Amycolatopsis orientalis NR /37(95%) Actinobacteria litter319 f: Micromonosporaceae (95%).3 Amycolatopsis vancoresmycina NR /39(95%) litter9 g: Mycobacterium (97%) Mycobacterium smegmatis X /37(%) litter22 g: Mycobacterium (%) Mycobacterium kansasii FR /3(95%) litter352 f: Mycobacteriaceae (%) <.1 <.1 <.1 Mycobacterium abscessus LC /3(95%) litter465 g: Mycobacterium (97%) <.1 <.1 Mycobacterium kansasii FR /43(97%)

3 Table S2. Biodiversity index of plant vegetation, bacteria, and AlkB-lineage like OTUs. Sample type Plant vegetation Bacteria OTUs of AlkBlineage Habitat Biodiversity Index Shannon-Weiner effective numbers of species Evenness Ravine Windward Leeward Ravine Windward Leeward Ravine Windward Leeward

4 Figure S1. Location of the sampling site (from Chao et al, 21). (a) Map of Taiwan. (b) Map of the Nanjenshan Reserve. (c) Stands set of windward habitat ( ) and Leewind habitat ( ) in Lanjenchi plot. (d) Stands set of ravine habitat ( ) in Nanjenshan plot I. (e) Stands set of ravine habitat ( ) in Nanjenshan plot II. This figure was produced with permission of original author Wei-Chun Chao who is the co-author of this study.

5 Detector Response (µv) 3 25 Ravine C Windward C Leeward C C C C 29 5 C 27 C 2 IS 5α C 3 C 32 C C 27 C 2 IS 5α C 3 C 32 C Time (min) C 27 C 2 IS 5α C 3 C 32 C Figure S2. Representative GC-FID chromatograms of aliphatic hydrocarbons of Iles rotunda in each habitat. Figure 1

6 litter33 34 litter litter357 litter litter95 15 litter39 litter litter311 litter13 litter5 litter17 litter514 litter11 litter127 litter157 litter2 9 Bradyrhizobium diazoefficiens AB litter6 litter137 litter31 74 litter litter49 litter36 2 litter litter112 litter litter Caulobacter crescentus AE73.1 litter litter26 litter14 litter litter1 litter15 74 litter litter67 24 litter litter11 litter6 1 litter365 litter144 litter23 Sinorhizobium meliloti AL Sinorhizobium meliloti CP litter464 litter Mesorhizobium ciceri CP Mesorhizobium amorphae NR litter117 litter44 5 Rhizobium etli U Brucella abortus strain CP Ochrobactrum anthropi CP75.1 litter19 litter9 litter5 Ensifer adhaerens CP litter14 Agrobacterium fabrum NR litter45 76 litter22 litter litter477 1 litter litter3 litter449 litter53 litter167 litter litter16 litter27 litter265 litter litter241 litter163 litter21 litter2 litter493 6 litter litter17 51 litter litter242 litter425 litter litter353 litter litter217 litter34 litter1 litter4 litter193 litter19 litter436 litter litter litter9 litter131 litter litter15 litter111 litter litter1 21 litter64 2 litter litter litter59 litter litter45 litter443 litter26 litter litter266 7 litter331 litter litter34 litter litter63 litter29 litter litter295 litter42 litter litter4 litter5 litter litter414 litter43 litter375 litter211 litter55 litter4 32 litter292 litter274 litter24 5 litter26 15 litter421 litter329 3 litter262 litter litter litter233 6 litter litter litter litter332 litter49 26 litter135 9 litter4 litter441 litter27 litter376 litter245 litter222 litter322 litter229 litter33 litter27 litter33 litter litter411 litter44 litter32 litter279 litter litter19 Stenotrophomonas maltophilia AM litter159 litter35 35 Xylella fastidiosa AE litter3 litter litter223 litter224 litter24 litter litter11 Burkholderia phenoliruptrix CP363.1 litter75 litter29 litter litter47 Burkholderia pseudomallei CP litter126 Ralstonia eutropha AM litter2 Ralstonia solanacearum AL litter34 35 Herbaspirillum seropedicae CP litter249 litter423 Achromobacter xylosoxidans CP Bordetella parapertussis HE53.1 Bordetella pertussis CP litter275 litter44 39 Comamonas testosteroni CP litter2 Delftia acidovorans CP4.1 litter273 litter346 litter412 Acinetobacter radioresistens KX Acinetobacter schindleri CP Moraxella catarrhalis CP25.1 Pseudomonas syringae CP75.1 Pseudomonas syringae NR Pseudomonas protegens strain AJ Pseudomonas putida LT739.1 litter55 Pseudomonas fluorescens CP315.1 Pseudomonas fluorescens AM Pseudomonas stutzeri CP34.1 Pseudomonas stutzeri CP Pseudomonas mendocina CP6.1 Pseudomonas aeruginosa CP litter145 Vibrio vulnificus BA Vibrio parahaemolyticus BA31.2 Vibrio cholerae CP Photobacterium leiognathi AY Pseudoalteromonas haloplanktis CR Yersinia enterocolitica CP Serratia plymuthica CP113.1 Serratia marcescens HG Klebsiella pneumoniae CP32.1 Enterobacter sacchari CP Salmonella enterica CP Salmonella enterica LT.1 Enterobacter cloacae NR Erwinia amylovora FN litter21 Enterobacter lignolyticus CP litter litter31 litter451 litter litter17 litter litter litter25 37 litter461 litter1 litter litter31 9 litter42 litter174 litter45 36 litter29 litter36 42 litter23 9 litter litter litter litter497 litter27 litter litter361 litter345 litter24 litter litter47 12 litter315 litter431 litter litter litter42 litter369 litter7 litter444 litter25 litter litter42 litter416 litter29.5

7 Mycobacterium tuberculosis (KY45.1) Mycobacterium intracellulare (X ) Mycobacterium leprae (X539.1) Mycobacterium avium (CP2215.1) 7 Mycobacterium bovis (AB ) 62 Mycobacterium kansasii (FR2239.1) litter465 litter22 Mycobacterium smegmatis (X ) litter Mycobacterium sp. (U ) 3 Mycobacterium fortuitum (GU ) 3 Mycobacterium chelonae (JX ) Mycobacterium abscessus (LC145.1) litter352 Corynebacterium sp. (KP ) 7 Corynebacterium jeikeium (NR ) 2 Corynebacterium simulans (NR ) Rhodococcus pyridinivorans (NR ) 14 6 Rhodococcus ruber (NR ) Rhodococcus aetherivorans (KR9251.1) 52 Gordonia terrae (CP ) 27 Nocardia mikamii (EU4439.1) Rhodococcus fascians (X5324.1) Rhodococcus jostii (NR ) 71 Rhodococcus erythropolis (AF ) litter325 litter litter1 litter litter264 litter litter26 12 litter319 litter32 1 litter litter litter69 45 Amycolatopsis orientalis (NR ) Amycolatopsis japonica (NR 251.1) 9 Amycolatopsis vancoresmycina (NR 255.1) Amycolatopsis mediterranei (NR ) 7 litter16 57 litter213 5 litter4 79 Rothia dentocariosa (AY ) Paenarthrobacter aurescens (KU ) Clavibacter michiganensis (NR ) litter litter476 litter65 litter litter12 34 litter149 litter73 5 litter litter259 3 litter5 97 Streptomyces xanthophaeus (EU ) 95 Streptomyces virginiae (NR ) 54 litter426 9 Streptomyces rimosus (FJ719.1) Streptomyces griseorubens (KX1364.1) 43 Streptomyces hygroscopicus (FJ7176.1) 37 6 litter23 litter261 litter litter litter2 6 litter6 litter litter36 litter25 litter226 litter164 litter93 53 litter237 litter35 5 litter252 litter46 74 litter4 litter23 95 litter litter litter92 litter141 litter221 5 litter12 litter41.2

8 (a) (b) (c) % 9% % 7% 6% 5% 4% 3% 2% 1% % Alphaproteobacteria Actinobacteria Sphingobacteriia unknown Deltaproteobacteria Bacteroidetes_incertae_sedis Gammaproteobacteria Acidobacteria_Gp4 Planctomycetia Flavobacteriia Betaproteobacteria Acidobacteria_Gp3 Opitutae Acidobacteria_Gp16 Chloroplast Chloroflexia Gemmatimonadetes Clostridia Acidobacteria_Gp1 Bacteroidia Spartobacteria Fimbriimonadia Cytophagia Ktedonobacteria Bacilli % 9% % 7% 6% 5% 4% 3% 2% 1% % Actinomycetales Rhizobiales Sphingomonadales unknown Sphingobacteriales Bdellovibrionales Myxococcales Xanthomonadales Rhodospirillales Planctomycetales Acidimicrobiales Caulobacterales Pseudomonadales Flavobacteriales Rhodobacterales Burkholderiales Rhodocyclales Alphaproteobacteria_incertae_sedis Opitutales Syntrophobacterales Enterobacteriales Chloroflexales Gemmatimonadales Methylophilales Thermoanaerobacterales Bacteroidales Rickettsiales Solirubrobacterales Bacillales Chromatiales Clostridiales Cytophagales Desulfobacterales Fimbriimonadales Ktedonobacterales Thiotrichales % 9% % 7% 6% 5% 4% 3% 2% 1% % Micromonosporaceae Rhizobiaceae Hyphomicrobiaceae Kineosporiaceae Phyllobacteriaceae Xanthomonadaceae Methylocystaceae Bradyrhizobiaceae Xanthobacteraceae Rhodospirillaceae Pseudomonadaceae Rhodobacteraceae Nocardioidaceae Microbacteriaceae Aurantimonadaceae Rhodocyclaceae Iamiaceae Brucellaceae Opitutaceae Syntrophaceae Beijerinckiaceae Chloroplast Gemmatimonadaceae Thermomonosporaceae Hyphomonadaceae Micrococcaceae Cystobacteraceae Solirubrobacteraceae Labilitrichaceae Cytophagaceae Ectothiorhodospiraceae Geodermatophilaceae Peptococcaceae_2 Ruminococcaceae Syntrophomonadaceae Thiotrichaceae Figure S5. Microbial community structure in the three habitats of Nanjenshan Reserve. Bacterial lineages were indicated in (a) class, (b) order, and (c) family. Sphingomonadaceae unknown Pseudonocardiaceae Chitinophagaceae Bdellovibrionaceae Polyangiaceae Sphingobacteriaceae Planctomycetaceae Methylobacteriaceae Caulobacteraceae Cryptosporangiaceae Acidimicrobiaceae Streptomycetaceae Flavobacteriaceae Comamonadaceae Acetobacteraceae Cryomorphaceae Mycobacteriaceae Kofleriaceae Acidimicrobineae_incertae_sedis Enterobacteriaceae Chloroflexaceae Thermoanaerobacteraceae Burkholderiaceae Marinilabiliaceae Rickettsiaceae Rhizobiales_incertae_sedis Oxalobacteraceae Conexibacteraceae Desulfobacteraceae Fimbriimonadaceae Ktedonobacteraceae Rhodobiaceae Sinobacteraceae Thermoactinomycetaceae_1

9 Figure S6. The distribution of OTUs and their relative abundance of microbial Actinobacteria. Size index of diameter vs relative abundance was showed at up-right panel.

10 Figure S7. The distribution of OTUs and their relative abundance of microbial Proteobacteria. Star symbol ( ) indicates the location with multiple OTUs. Size index of diameter vs relative abundance was showed at up-right panel.