SYMMETRY FOAMING HAND SANITIZER with Aloe & Vitamin E Technical Data

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1 508 SYMMETRY FOAMING HAND SANITIZER with Aloe & Vitamin E Technical Data Physical Properties Active Ingredient: Ethyl Alcohol 62% (70% v/v) Appearance: Clear, Colorless Solution Fragrance: Floral Form: Liquid ph: Preservative Testing Determination of the effectiveness of the preservative system against repeated bacterial, yeast, and mold challenges. United States Pharmacopeia/ National Formulary Antimicrobial Effectiveness (Preservative) Test <51> bacteria, yeast, and mold to confirm the preservative system will kill these contaminants. The USF/NF protocol was modified to include repeat challenges. The study included all appropriate sterility and neutralization controls. Test Organisms: Staphylococcus aureus ATCC 6538 Escherichia coli ATCC 8739 Pseudomonas aeruginosa ATCC 9027 Candida albicans ATCC Aspergillus niger ATCC (continued on back of page) Clinical Research Laboratories, Inc. (Piscataway, NJ) Met and exceeded USP/NF performance requirements after each challenge.

2 Repeat Insult Patch Test Determination of potential for dermal irritation or sensitization. Repeat Insult Patch Test Shelanski Method Nine applications of product were conducted during the Induction Phase. Applications included applying the product to the same skin site of at least 50 human subjects for 24 hours. Sites were evaluated for irritation throughout the study. After a day rest period, product application was repeated on a new skin area to assess the potential for sensitization. Clinical Research Laboratories, Inc. (Piscataway, NJ) No irritation or sensitization reactions were observed throughout the study. Antimicrobial Effectiveness Studies Laboratory: Evaluation of antimicrobial effectiveness when challenged with a broad spectrum of microorganisms. American Society of Testing & Materials E2315 Standard Guide for Assessment of Microbiocidal Activity Using a Time-Kill Procedure microorganisms to confirm kill rates after 15 and 30 second contact. Studies included all appropriate sterility and neutralization controls. ATS Labs 1285 Corporate Center Drive, Suite 110 Eagan, MN

3 Microorganism Identification No. Percent Reduction 15 Seconds 30 Seconds Acinetobacter baumannii ATCC > > Clostridium difficile ATCC 9689 >99.96 >99.96 (vegetative) Enterobacter aerogenes ATCC > > Enterococcus faecalis ATCC > > Vancomycin resistant (VRE) [1] Enterococcus faecium ATCC 6569 > > Escherichia coli ATCC > > Escherichia coli O157:H7 ATCC > > Klebsiella pneumoniae ATCC > > Listeria monocytogenes ATCC > > Proteus mirabilis ATCC 9240 > > Pseudomonas aeruginosa ATCC > > Salmonella enteritidis ATCC 4931 > > Salmonella typhimurium ATCC > > Serratia marcescens ATCC > > Shigella dysenteriae ATCC > > Shigella sonnei ATCC > > Staphylococcus aureus ATCC > > MRSA [2] Staphylococcus aureus CA-MRSA [3] (Community Acquired MRSA) Staphylococcus aureus VRSA Staphylococcus epidermidis Streptococcus pneumoniae PRSP Streptococcus pyogenes (Group A) NARSA NRS123 (USA400) > > NARSA VRS1 > > ATCC > > ATCC > > ATCC > > ATCC - American Type Culture Collection NARSA - Network on Antimicrobial Resistance in Staphylococcus aureus repository [1] This test strain was confirmed resistant to Vancomycin, Streptomycin, Quinupristin and Dalfopristin. [2] This test strain was confirmed resistant to Methicillin, Clindamycin, Oxacillin, Penicillin, and Tetracycline. [3] This test strain was confirmed resistant to Methicillin, Oxacillin, and Penicillin. (continued on back of page) 3

4 Laboratory: American Society of Testing & Materials E1052 Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension microorganisms to confirm kill rates after 15 and 30 second contact. Studies included all appropriate sterility and neutralization controls. ATS Labs 1285 Corporate Center Drive, Suite 110 Eagan, MN Microorganism Identification No. Percent Reduction 15 Seconds 30 Seconds Herpes Simplex Virus ATCC VR Type 1 HIV-1 HTLV-III B Strain* Influenza A/Hong ATCC VR Kong Swine Influenza A (H1N1) virus ATCC VR-333 ATCC - American Type Culture Collection *Advanced Biotechnologies, Columbia, MD Detection of Holes in Medical Gloves To determine compatibility of the hand sanitizer with latex, vinyl, and nitrile gloves. Detection of Holes in Medical Gloves (ASTM D5151) 4

5 Detection of Holes in Medical Gloves (Continued) One hundred gloves of each of the three types were conditioned in the hand sanitizer for 2 hours and then examined for leaks. Control gloves (one hundred of each type) that had not been exposed to product were also checked for leaks. Smithers Rapra Inc. (Akron, OH) No degradation of medical gloves was detected when compared to control. ISSUED: 11/19/13 Supersedes: 2/14/11 5

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