ANNEX B. EPIC DATABASE RADIATION EFFECTS ON AQUATIC ORGANISMS (RUSSIAN/FSU DATA) EPIC Deliverable 5. ANNEX B. 1

Transcription

1 ANX B. EPIC DATABASE RADIATION EFFECTS ON AQUATIC ORGANISMS (RUSSIAN/FSU DATA) EPIC Dlivrabl 5. ANX B. 1

2 RADIATION EFFECTS ON AQUATIC ORGANISMS (RELEVANT TO NORTHERN AREAS, RUSSIAN DATA), CHRONIC AND ACUTE EXPOSURE. Effct cods: -o ffct; -ffct o rproductio;mt-ffct o mortality;mb-ffct o morbidity; AD-adaptatio to radiatio; STIM- stimulatio. -prlimiary dos stimats mad by th authors th databas. A1-1 Fish Cyprius carpio. Carp (1 yar old) A1-2 Fish Cyprius carpio. Carp (1 yar old) A1-3 Fish Cyprius carpio. Carp ( 1 yar old) Aquarium xprimt, chroic xposur from Sr-90. Tst: phagocytic activity lucocyts o xprimtal ifctio with bactria. Exposur 15 days Aquarium xprimt, chroic xposur from Sr-90. Tst: phagocytic activity lucocyts o xprimtal ifctio with bactria. Exposur 30 days Aquarium xprimt, chroic xposur from Sr-90. Tst: Sr-90 1,85E+03 1,85E+05 (bos, quilibriu m ) Sr-90 1,85E+03 1,85E+05 (bos, quilibriu m) Sr-90 1,85E+03 1,85E+05 (bos, quilibriu m) 3,3E-05 (kidy) 8,0E-05 (kidy) 8,9E-4 (kidy) 2,0E-04 (kidy) 1,2E-3 (kidy) 4,2E-02 (kidy) Phagocytic rspos lucocyts o ifctio did ot diffr from th rspos i th cotrol.cotrol rspos: maximum umbr fagocytig lucocyts was 28% 1.5 hours aftr ifctio. Th ovrall rspos o ifctio was lowr tha i th cotrol, maximum phagocytic activity was 17% o hour aftr ifctio (cotrol - 28%). Th ovrall rspos o ifctio was lowr tha i th cotrol, maximum phagocytic activity was 17-18% durig th 2d hour aftr ifctio (cotrol - 28%). MB MB Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i EPIC Dlivrabl 5. ANX B. 2

3 A1-4 Fish Cyprius carpio. Carp ( 1 yar old) A2-1 Fish Cyprius carpio. Carp ( 1 yar old) phagocytic activity lucocyts o xprimtal ifctio with bactria Aromoas puctata. Radiatio xposur 90 days Aquarium xprimt, chroic xposur from Sr-90. Tst: phagocytic activity lucocyts o xprimtal ifctio with bactria Aromoas puctata. Radiatio xposur 180 days Aquarium xprimt, chroic xposur from Sr-90. Tst: phagocytic activity lucocyts o xprimtal Sr-90 1,85E+03 1,85E+05 (bos, quilibriu m) Sr-90 3,70E+04 3,7E+06 (bos) (quilibri um *) 6,9E-4 (kidy) 2,7E-03 (kidy) 0,2 (kidy) 1,9E-02 (kidy) Th rspos o ifctio was mor activ tha i th cotrol, maximum phagocytic activity was 32% i 1.5 hours aftr ifctio (cotrol - 28%). Th ovrall rspos o ifctio was lowr tha i th cotrol, maximum phagocytic activity was 17% o hour aftr ifctio (cotrol - 28%). AD MB Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 EPIC Dlivrabl 5. ANX B. 3

4 A2-2 Fish Cyprius carpio. Carp ( 1 yar old) A2-3 Fish Cyprius carpio. Carp ( 1 yar old) ifctio with bactria Aromoas puctata. Radiatio xposur 15 days Aquarium xprimt, chroic xposur from Sr-90. Tst: phagocytic activity lucocyts o xprimtal ifctio with bactria Aromoas puctata. Radiatio xposur 30 days Aquarium xprimt, chroic xposur from Sr-90. Tst: phagocytic activity lucocyts o xprimtal ifctio with bactria Aromoas puctata. Sr-90 3,70E+04 3,7E+06 (bos) (quilibri um *) Sr-90 3,70E+04 3,7E+06 (bos) (quilibri um *) 6,6E-03 (kidy) 1,5E-02 (kidy) 8,2E-02 (kidy) 0,7 (kidy) Th ovrall rspos o ifctio was lowr tha i th cotrol, maximum phagocytic activity was 15% o hour aftr ifctio (cotrol - 28%). Th ovrall rspos o ifctio was lowr tha i th cotrol, maximum phagocytic activity was 20% o hour aftr ifctio (cotrol - 28%). MB MB Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 EPIC Dlivrabl 5. ANX B. 4

5 A2-4 Fish Cyprius carpio. Carp ( 1 yar old) A3 Fish Cyprius carpio. Carp (1 yar old) Radiatio xposur 90 days Aquarium xprimt, chroic xposur from Sr-90. Tst: phagocytic activity lucocyts o xprimtal ifctio with bactria Aromoas puctata. Radiatio xposur 180 days Aquarium xprimt, chroic xposur from Sr-90. Tst: atibody productio o rpatd immuizatio with bactrial atig (by th ractio agglutiatio). Radiatio xposur 90 days Sr-90 3,70E+04 3,7E+06 (bos) (quilibri um *) Sr-90 1,85E+03 1,85E+05 (bos) (quilibri um *) 2,9E-02 (kidy) 0,001 (kidy) 2,75 (kidy) 0,04 (kidy) Th ovrall rspos o ifctio was lowr tha i th cotrol, maximum phagocytic activity was 17-18% i th priod hours aftr ifctio (cotrol - 28%). Wakig immu activity: dlay ad rductio atibody productio o immuizatio with bactrial atig. Agglutiis appard o 21st day (2 wks latr tha i th cotrol). Maximal titrs agglutiis (1:640-1:1260) wr dtctd o days, i.. th pak rspos was about 2 tims lowr ad dlayd comparig with th cotrol rspos.cotrol rspos: atibodis appard o th 7th day aftr immuizatio, maximum titr agglutiis (1:2670) was dtctd o 36-th day. MB MB Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 A4 Fish Cyprius Aquarium Sr-90 3,70E+04 3,7E+06 0,015 0,7 Wakig immu activity: dlay MB Shlifr & EPIC Dlivrabl 5. ANX B. 5

6 carpio. Carp (1 yar old) A5-1 Fish Cyprius carpio Carp A5-2 Fish Cyprius carpio. Carp (1 yar old) A5-3 Fish Cyprius carpio. Carp (1 yar old) xprimt, chroic xposur from Sr-90. Tst: atibody productio o rpatd immuizatio with bactrial atig (by th ractio agglutiatio). Radiatio xposur 90 days Aquarium xprimt, chroic xposur, 30 days. Tst: bactriostatic proprtis blood srum Aquarium xprimt, chroic xposur, 180 days. Tst: bactriostatic proprtis blood srum. Aquarium xprimt, chroic xposur, 270 days. Tst: (bos) (quilibri um *) Sr-90 1,85E (bos, rcostru ctio) Sr-90 1,85E E+4 Sr-90 1,85E+03 3,7E+4 (bos, *) (kidy) (kidy) ad rductio atibody productio o immuizatio with bactrial atig. Agglutiis appard o 21st day (2 wks latr tha i th cotrol). Maximal titrs agglutiis (1:173-1:1280) wr dtctd o days, ths titrs wr about 2 tims lowr ad moths dlayd th cotrol rspos. Cotrol rspos: atibodis appard o th 7th day aftr immuizatio, maximum titr agglutiis (1:2670) was dtctd o 36-th day. 8,0E-05 (kidy) 0,0009 (kidy) 0,0007 (kidy) 1,20E-03 from 1,2E-03 to 0,2 Stimulatio bactriostatic proprtis blood srum durig th first 30 days radiatio xposur. No diffrcs with th cotrol i bactriostatic proprtis blood srum i th priod from 30 up to 180 days radiatio xposur. 0,2-0,34 Gradual dtrioratio bactriostatic proprtis blood srum at doss gratr tha 0.2 Gy. At th d xprimt th bactriostatic activity was % lowr tha th cotrol STIM MB Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977,1980; rviwd i Shkhaova,1983 (p.111) Shlifr & Shkhaova, 1977,1980; rviwd i Shkhaova,1983 (p.111) Shlifr & Shkhaova, 1977, 1980; rviwd i Shkhaova,1983 (p.111) EPIC Dlivrabl 5. ANX B. 6

7 A6-1 Fish Cyprius carpio. Carp (1 yar old) A6-2 Fish Cyprius carpio. Carp (1 yar old) A6-3 Fish Cyprius carpio. Carp (1 yar old) A7 Fish Misgurus fossilis Loach (adult) bactriostatic proprtis blood srum. Aquarium xprimt, chroic xposur, 15 days. Tst: bactriostatic proprtis blood srum. Aquarium xprimt, chroic xposur, 150 days. Tst: bactriostatic proprtis blood srum Aquarium xprimt, chroic xposur, 270 days. Tst: bactriostatic proprtis blood srum Aquarium xprimt, chroic xposur, 90 days Sr-90 3,70E (bos, *) 0,003 (kidy) Sr-90 3,70E+04 3,70E+06 0,02 (kidy) Sr-90 3,70E (bos, *) Sr-90 1,85E+03 3,7E+04 (bos, *) 0,03 (kidy) 2E-03 (prlimi ary stimatio ) 0,02 Stimulatio bactriostatic proprtis blood srum durig th first 15 days radiatio xposur. 0,02-0,5 No diffrcs with th cotrol i bactriostatic proprtis blood srum i th priod from 15 up to 150 days radiatio xposur. 0,5-5,3 Gradual dtrioratio bactriostatic proprtis blood srum at doss gratr tha 0.5 Gy. At th d xprimt th bactriostatic activity was % lowr tha th cotrol 0,04 (0,2 rcostru ctio) Ngativ biochmical chags i goads mals. Rductio to zro th glycog coctratio i goads (cotrol glicog coctratio 5-7 mg/gramm goads). Fatty tsticls (up to 95 mg/g with a cotrol 26 mg/g). No similar ffcts wr obsrvd i fmals. STIM MB MB Shlifr & Shkhaova, 1977, 1980; rviwd i Shkhaova,1983 (p ) Shlifr & Shkhaova, 1977, 1980; rviwd i Shkhaova,1983 (p.111) Shlifr & Shkhaova, 1977, 1980; rviwd i Shkhaova,1983 (p.111) Shkhaova,Blma kov, Lapi,1969; rviwd i Shkhaova,1983 (p.114) A8 Fish Misgurus Aquarium Sr-90 3,70E+04 3,7E+06 8E-03 0,7 Ngativ biochmical chags i MB Shkhaova,Blma EPIC Dlivrabl 5. ANX B. 7

8 fossilis Loach (adult) A9 Fish Cyprius carpio Carp (1 yar old) A10 Fish Ctophary godo idlla. Grass carp (fry) A11 Fish Rutilus rutilus Roach xprimt, chroic xposur, 90 days Aquarium xprimt, chroic xposur, 360 days Aquarium xprimt, chroic xposur, 90 days Watr rsrvoir cotamiatd from idustrial activity PA Mayak, southr Urals. Fild study (N about 1500). Sr-90 (3,7-7,4)E+02 (bos) (quilibri um *) (3,7-7,4)E+04 (bos, *) Sr-90 4,07E Sr- 90,Cs- 137 (5,9-7,4)E+03 ( 90 Sr); ( ) ( 137 Cs) 1,5E+06 ( 90 Sr i bos, d.w.); 1,6E+04 ( 90 Sr i muscls); 1,4E+04 (90Sr i goads); 7,8E+04 (137Cs i muscls, (prlimi ary stimatio ) 0,003 (livr) 0,0015 (muscls) 1 (livr); 0.5 (muscls) 0,03 (y) >1,5 (y) 7E-03 (itral); 1,5E-02 (bos); 0,0048 (xtral) 4,4 (pr yar, ) tsticls mals. Rductio to zro th glycog coctratio i goads (cotrol glicog coctratio 5-7 mg/gramm goads). Fatty tsticls (up to 127 mg/g with a cotrol 26 mg/g). Ngativ biochmical chags i th livr ad muscls: icrasig coctratio lipidoproxids.coctratio lipoproxids i xposd fish: livr 18.98± 0.81 Mol/mg lipids (cotrol 5.9±0.8 Mol/mg lipids);muscls 15.94± 1.43 Mol/mg lipids (cotrol 3.48±0.85 Mol/mg lipids) Pathological dtrioratio ysight. Effct was obsrvd sic 50-th day xprimt. Edma crystalli ls ad rtia th y, dystrophic dgratio th crystalli substac, disturbac th structur photorcptors. Frtility roach dcrasd almost by a factor 2 as compard to roach from th cotrol watr body. Spawig occurd latr tha i th cotrol. Thr wr o fish oldr tha 9 yars i th spawig shoal. Goads had ormal structur, but th umbr sxual clls was lowr tha th orm. Th growth fish did ot diffr from th cotrol. MB MB kov, Lapi,1969; rviwd i Shkhaova,1983 (p.114) Storozhuk & Shkhaova, 1977; rviwd i Shkhaova,1983 (p.114) Nilov, Fdosko, Shkhaova,1976; rviwd i Shkhaova,1983 Pshkov t.al., 1978 EPIC Dlivrabl 5. ANX B. 8

9 A12 Fish Carassius auratus giblio Goldfish A13 Fish Carassius auratus giblio Goldfish A14 Fish Carassius auratus giblio Goldfish A15 Fish Rutilus rutilus Roach Lak (prsumably Brdish), cotamiatd i 1957 (Kyshtym accidt), southr Urals. Fild studis 1970s. Lak (prsumably Uruskul), cotamiatd i 1957 (Kyshtym accidt), southr Urals. Fild studis 1970s. Lak (prsumably Uruskul), cotamiatd i 1957 (Kyshtym accidt), southr Urals. Fild studis Watr rsrvoir cotamiatd from idustrial activity PA Sr- 90,Cs- 137 Sr- 90,Cs- 137 Sr- 90,Cs- 137 Sr- 90,Cs ,17E+03 ( 90 Sr) w.w.) (5.6-11)E+04 (bos) 1,48E+03 (3,7-5,5)E+05 (bos) 1,50E+03 (3,7-5,5)E+05 (5,9-7,4)E+03 ( 90 Sr); ( ) ( 137 Cs) 1,5E+06 ( 90 Sr i bos, d.w.); 1,6E+04 5,00E-04 (3,0-5,0)E-03 (3,0-5,0)E-03 7E-03 (itral); 1,5E-02 (bos); 0,0048 0,2 (Gy pr yar, ) 1,1-1,8 (Gy pr yar, ) 1,1-1,8 (Gy pr yar, ) 4,4 (Gy pr yar, ) No spcims oldr tha 8 yars wr prst i th catchs, which is ot typical th giv spcis. Grow-up 4-7 yar old spcims domiatd. Morphological aomalis wr obsrvd i 24 % th fish xamid. For th most part, ths wr spcims with upaird goads, % ths spcims wr stril. No spcims oldr tha 8 yars wr prst i th catchs, which is ot typical th giv spcis. Spcims 4-6 yars ag domiatd i th spawig shoal Morphological aomalis wr obsrvd i 15 % th fish. Ths wr maily aomalis goad structur (upaird goads, strility). Morphological aomalis wr obsrvd i 6-9 % th grow-up fish, icludig % with udrdvlopd goads or sigs hrmaphrodit. I th cotrol thr MT Voroia E.A., Pshkov S.P., I.A. Shkhaova,1977; Kryshv, 2002 (doss) Voroia E.A., Pshkov S.P., I.A. Shkhaova,1977; Kryshv, 2002 (doss) Voroia E.A., Pshkov S.P., I.A. Shkhaova,1977 Ermokhi, Mutia, 1977 EPIC Dlivrabl 5. ANX B. 9

10 A16 Fish Carassius auratus giblio Goldfish A17 Fish Carassius carassius Goldfish A18 Fish Pik Mayak, southr Urals. Fild study (N about 1500). Lak (prsumably Brdish), cotamiatd i 1957 (Kyshtym accidt), southr Urals. Fild studis Lak (prsumably Brdish), cotamiatd i 1957 (Kyshtym accidt), southr Urals. Fild study Watr rsrvoir ( 90 Sr i muscls); 1,4E+04 (90Sr i goads); 7,8E+04 (137Cs i muscls, w.w.) Sr-90 1,20E+03 (9.6±0.4) E+04 ( 90 Sr, bos) Sr-90 1,20E+03 (9.6±0.4) E+04 ( 90 Sr, bos) Sr- 90,Cs- 7,4E+03 ( 90 Sr); E+05 ( 90 Sr, (xtral) 5,00E-04 5,00E-04 0,002 () 0,2 (Gy pr yar, ) 0,2 (Gy pr yar, ) 0,7 (Gy pr yar, wr % th fish with morphological aomalis. a)aomalis i th body structur: 13.4 % spcims had o ovary, 18.2 % spcims wr without goads, 18 % spcims had morphological aomalis : curvatur fis ad th tail part, irrgular structur scals (N=358). O artificial icubatio ro 40 % ormal mbryos wr producd. This valu was ot cosidrd as low for th giv spcis (Mutia, 1977). B) Morphological abormalitis wr obsrvd i 24% fish xamid: stril wr 17%(3+) ad up to 25% (4+ ad oldr) spcim (Voroia, Pshkov, Shkhaova, 1977). O artificial icubatio ro 51 % ormal mbryos wr producd. This valu was ot cosidrd as low for th giv spcis. With rspct to th coditio ro ad th dvlopmt mbryos, th Mutia, 1977; Voroia, Pshkov, Shkhaova, 1977 Mutia, 1977 Mutia, 1977 EPIC Dlivrabl 5. ANX B. 10

11 A19 Fish Prca fluviatilis Prch A20 A21 Mollu sc Shllf ish Mollusc - pod sail Crayfish A22 Fish Hypophthal michthys molitrix Silvr carp cotamiatd from idustrial activity PA Mayak, southr Urals. Fild study Watr rsrvoir, cotamiatd i 1957 (Kyshtym accidt), southr Urals. Fild studis 1970s. Exprimt i a pod, chroic, duratio 4 moths Exprimt i a pod, chroic, 4 moths Coolig Pod th Chrobyl NPP, cotamiatd i 1986.Fild 137 ( 137 Cs) bos); 1.9E+05 ( 137 Cs, muscls) Sr- 90,Cs- 137 Mixtur fissio product s, 90 Sr domiat s Mixtur fissio product 90 Sr 7,4E+03 ( 90 Sr); 93 ( 137 Cs) ~3,7E+03 ~3,7E+03 (1,1-11,5)E+0 5 ( 90 Sr, bos); (3,2-4,5)E+05 ( 137 Cs, muscls) about 1E+05 ( prlimia ry stimatio ) about 1E+05 ( prlimia ry stimatio ) s, domiat s Cs ± (muscls) 0,0026, () 1E-03-1E-02 () 1E-03-1E-02 () 0,0004 ( ) ) rproductio was qualitativly stimatd as ormal. 1 (pr yar, ) 4 ( ), With rspct to th coditio ro ad th dvlopmt mbryos, th rproductio was qualitativly stimatd as ormal. Th rprstativs bthos (podsails ad dtats did durig th summr saso obsrvatios. Crayfish did durig th summr saso, part th spcims lft th xprimtal pod. Thr wr 5.7 % stril spcims 8.6 % spcims with th goad asymmtry. Accordig to th data cytological aalysis, 25 % mals had aomalis sxual clls. I th cotrol populatio lss tha 0.25 % Mutia, 1977 MT Mary, 1976 MT Mary, 1976 Blova t.al, 1993; Kryshv t al., 1996 (doss); Kryshv, 1998 (doss) EPIC Dlivrabl 5. ANX B. 11

12 A23 Fish Hypophthal michthys molitrix Silvr carp A24 Fish Hypophthal michthys molitrix. Silvr carp A25 Fish Hypophthal michthys molitrix. Silvr carp A26 Fish Hypophthal michthys molitrix. Silvr carp A27 Fish Hypophthal michthys molitrix. Silvr carp study 1989 Coolig Pod th Chrobyl NPP, cotamiatd i Fild study 1990 Coolig Pod th Chrobyl NPP, cotamiatd i Fild study i 1991 Coolig Pod th Chrobyl NPP, cotamiatd i Fild study i 1992 Coolig Pod th Chrobyl NPP, cotamiatd i Fild study i 1992 Coolig Pod th Chrobyl NPP, accidtal cotamiatio Cs ± (muscls) Cs-137 (also othr radiou clids) (muscls) 0,0004, 0,0004, Cs ,0004, Cs ,0004, Cs (muscls) (4-5)E-04 4,3 ( ), 4,5 ( ), 4,6 ( ), 4,6 ( ), 0,18 (pr yar i ) spcims wr stril. Thr wr 12.5 % stril spcims ad 16.7 % spcims with th goad asymmtry. Accordig to th data cytological aalysis, 47.1 % fish had aomalis sxual clls. Thr wr 23.1 % spcims with th goad asymmtry. No stril spcims wr dtctd. Accordig to th data cytological aalysis, 68.8 % fish had aomalis sxual clls. Th data ivstigatio fish from fishig cribs: 42.9 % mals had a dformd shap goads. Accordig to th data cytological aalysis, 100 % mals ad 33.3 % fmals had aomalis sxual clls. Th data ivstigatio frlylivig fish from th coolig pod th Chrobyl NPP: 15.4 % mals wr partially stril, ad 9.1 % fmals had th goad asymmtry. Accordig to th data cytological aalysis, 89.5 % fish had aomalis sxual clls. Th fsprig from th first gratio silvr carps that survivd th Chrobyl accidt, th yar birth 1989, wr grow i fishig-cribs i th coolig pod th Chrobyl NPP. I 1992, 28.7 % fish had Blova t.al, 1993; Kryshv t al., 1996 (doss); Kryshv, 1998 (doss) Blova t.al, 1993; Kryshv t al., 1996 (doss); Kryshv, 1998 (doss) Blova t.al, 1993; Kryshv t al., 1996 (doss); Kryshv, 1998 (doss) Blova t.al, 1993; Kryshv t al., 1996 (doss); Kryshv, 1998 (doss) Makva t al.,1994 EPIC Dlivrabl 5. ANX B. 12

13 A28 Fish Hypophthal michthys molitrix. Silvr carp A30 A31 A32 Zoopl Zoopl Zoopl i Fild study, Coolig Pod th Chrobyl NPP, cotamiatd i Fild study, Cs (muscls) Daphia Exprimt Sr-90 (3.7-37)E+05 Daphia Exprimt Sr-90 ( )E+03 (3.7-37)E+7, ( )E+5, prlimia ry stimatio (4-5)E-04 0,57-5,7, 0,0057-0,57, Daphia Exprimt Sr-90 3,70 370, 0, , 0,18 (pr yar i ) aomalis, icludig 2.8 % stril bisxual spcims, 11.1 % with aomalis th goad shap, 8.3 % with aomalis th body shap, 3.7 % with aomalis th swimmig bladdr, ad 2.8 % with th othr aomalis. Th fsprig from th first gratio silvr carps that survivd th Chrobyl accidt, th yar birth 1990, wr grow i fishig-cribs i th coolig pod th Chrobyl NPP. I 1992, 12.1% fish had aomalis, icludig 3.2 % with aomalis th goad shap ad 8.9 % with aomalis th body shap. No stril spcims wr obsrvd i this gratio. Accordig to th data cytological aalysis, all spcims had aomalis i sxual clls, ad th umbr aomalous sxual clls ragd from1 % to 20 %. Liftim Daphia s dcrasd by days as compard to th cotrol. Th umbr youg Daphia icrasd i th first two days; th supprssio maifstd itslf, ad th umbr molts ad youg crustacas dcrasd. A wak stimulatig ffct was obsrvd i Daphia s. MT MT STIM Makva t al.,1994 Lbdva,1957 Lbdva,1957 Lbdva,1957 A33 Fish Watr Sr- 7,8E+04 (6,5 ± 2,7 (pr O artificial icubatio ro th Smagi,1996 EPIC Dlivrabl 5. ANX B. 13

14 A34 Fish A35-1 Fish A35-2 Fish A35-3 Fish A36 Fish Pik Pik Pik Pik Pik Salmo salar. Salmo body,cotami atd from idustrial activity PA "Mayak", Southr Urals, Russia, watr rsrvoir N.10.Fild studis Exprimt, artificial icubatio ro, 8 days Exprimt, artificial icubatio ro, 8 days Exprimt, artificial icubatio ro, 8 days Exprimt, artificial icubatio ro, 8 days Exprimt, artificial icubatio ro, up to ,Cs- 137 Sr-90 + Cs-137 Sr-90 14,8E+03 90Sr ad Cs. <3,7E+05 (Sr-90)+ 3,7E+04 (Cs-137) <1,85E+0 7 (Sr-90); 1,3E+05 (Cs-137) i adult pik 2)E-03 ay from b- radiatio (1 ± 0.3)E-03 ay from g- radiatio (goads adult piks) 5E-03 () <0,24 yar, itral), 4E-02 () <3,36 (xtral, rcostru ctio) Sr-90 3,70E+07 0,47 6,58 (xtral, rcostru ctio) Sr-90 Sr-90- Y-90 >=7,4E+0 7 3,7 4,7E-08 (xtra l, t prctag mbryoss with abormalitis was 10 tims highr (13%) tha i th cotrol (1%).Ni typs various dformitis occurrd i th cotamiatd watr body ad o-two typs i th cotrol. Forlarva with aomalis dvlopmt did i th first moth lif, i.. wr cut f by atural slctio Stimulatio mbryoic dvlopmt irradiatd fish ad arlir hatchig for-larva: o th 8th day hatchig i th cotrol was 18,8%; i xprimtal sris %. Numbr aomalous or dad did ot diffr from cotrol. O artificial icubatio ro th prctag mbryos with abormalitis did't diffr statistically from that i th cotrol ( %). O artificial icubatio ro th prctag mbryos with abormalitis was 33.3% that is 7-8 tims highr tha i th cotrol ( %). 0,94 7,5 O artificial icubatio ro all mbryos had abormalitis ad forlarva did withi 5 days aftr hatchig. 6,0E-06 (xtral, rcostru ctio) O artificial icubatio ro a stimulatio rd blood dvlopmt i mbryos was obsrvd. Primary matur rithrocyts wr foud i STIM MT Pitkya,1971 Pitkya,1971 Pitkya,1971 Pitkya,1971 STIM Nustrov (1966) EPIC Dlivrabl 5. ANX B. 14

15 A37-1 Fish A37-2 Fish A37-3 Fish A37-4 Fish Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout days; T=1-2 C Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=200. Tst:dath fish Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=200 Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=200. Tst:dath fish Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=100. Tst: rcostr uctio) Co-60 9,25E+06 0,33 (*, xtral) Co-60 9,25E+05 0,032 (*, xtral) Co-60 3,70E+03 1,3E-04 (*, xtral) Co-60 3,70E+06 0,13(*, xtral) larg amouts o 109-th day i blood xprimtal mbryos (80% rd blood lmts); i th cotrol matur rithrocyts appard o 128-th day (20% rd blood lmts). Th dvlopmt xprimtal mbryos procdd mor rapidly, ad hatchig bga arlir tha i th cotrol. 17,2 Most part (80%) did bfor hatchig (cotrol: 16.5%; 0.75<P<0.85) 1,72 Up to 54% did bfor hatchig (cotrol: 16.5%; 0.47<P<0.61) 6,70E-03 No statistical diffrc with th cotrol i th umbr fish did bfor hatchig 6,9 Practically all (98%) for-larva did withi fw days aftr hatchig. MT MT MT Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) EPIC Dlivrabl 5. ANX B. 15

16 A37-5 Fish A38-1 Fish A38-2 Fish A38-3 Fish A38-4 Fish Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout survival for-larva Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=100. Tst: survival for-larva Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=100. Tst: survival for-larva Exprimt, artificial icubatio ro, up to 128 days; T=7-9 C Exprimt, artificial icubatio ro, up to 128 days; T=7-9 C. Tst:dath fish Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. Co-60 3,70E+06 0,13(*, xtral) M-54 3,70E+05 4,3*10(- 3) (xtral), M-54 1,48E+04 1,7*10(- 4) (xtral), M-54 3,70E+01 0, (xtral ), M-54 3,70E+05 0,0043(x tral), 6,9 Practically all (98%) for-larva did withi fw days aftr hatchig. 0,55 (xtral), 0,022 (xtral), 0,55 (xposur, rcostru ctio) Icrasd prctag fish did durig th artificial icubatio ro: 52% i th xprimt ad 16.5% i th cotrol. From survivd up to 14.5% larva had abormalitis (cotrol 3.8%) Icrasd prctag fish did durig th artificial icubatio ro: 26% i th xprimt ad 16.5% i th cotrol.from survivd th icrasd % abormalitis i larva was obsrvd - 9.4% (cotrol 3.8%). No statistical diffrc with th cotrol i th umbr fish did bfor hatchig Numbr did forlarva was somwhat highr (31-36%) tha that i th cotrol (21%).Diffrc was statisticaly rliabl. MT MT MT MT Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) EPIC Dlivrabl 5. ANX B. 16

17 A38-5 Fish A38-6 Fish A39-1 A39-2 A40-1 Fish Fish Fish Salmo iridus Gibbas. Raibow trout Salmo iridus Gibbas. Raibow trout Salmo salar. Salmo Salmo salar. Salmo Corgous pld. Pld N=100. Tst: survival for-larva Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=100. Tst: survival for-larva Exprimt, artificial icubatio ro, up to 52 days; T=7-9 C. N=100. Tst: survival for-larva Exprimt, artificial icubatio ro, up to 66 days Exprimt, artificial icubatio ro, up to 66 days Svral xprimts carrid out i 4 yars, artificial M-54 1,48E+04 1,7E- 04(xtr al), M-54 3,70E+01 0, (xtral ), Sr ,35E- 06 (xtra l, rcostr uctio) Cs ,3E-07 (xtra l, rcostr Mixtur s fissio product 1,11E+02-1,11E+08 uctio) 0,022 (xposur, rcostru ctio) 1,55E-04 (xtral, rcostru ctio) 5,5E-05 (xtral, rcostru ctio) No statistical diffrc with th cotrol i th arly dath forlarva No diffrc with th cotrol i th arly dath forlarva Mortality salmo ad larva was 1.5 tims highr tha i th cotrol. Mortality salmo ad larva was 2.4 tims highr tha i th cotrol >5 Th mortality fish icrasd with absorbd dos bgiig from th dos 5 Gy. At doss highr tha 300 Gy complt dath ro was MT MT MT Lyapi,Podgursky, Kyazva (1971) Lyapi,Podgursky, Kyazva (1971) Fdorov t.al. (1962). Fdorov t.al. (1962). Mashva & Sukalskaya (1973) EPIC Dlivrabl 5. ANX B. 17

18 A40-2 A41-1 A41-2 A41-3 Fish Fish Fish Fish Corgous pld. Pld Salmo salar. Salmo Salmo salar. Salmo Salmo salar. Salmo icubatio ro. T=2-4 C. Eggs wr icubatd from stag "lat gastrula" Svral xprimts carrid out i 4 yars, artificial icubatio ro. T=2-4 C. Eggs wr icubatd from stag "lat gastrula" Exprimt, artificial icubatio ro. 234 days Exprimt, artificial icubatio ro. 234 days Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. s (Y- obsrvd 131, Ba- 140+La -140, Ru-106) Mixtur >1,11E+0 >300 At doss highr tha 300 Gy complt s 8 dath ro was obsrvd fissio product s (Y- 131, Ba- 140+La -140, Ru-106) C-144 5,90E+04 0,001 0,234 Hatchig startd about 3-4 days arlir tha that i th cotrol ad was mor prologd i tim.numbr did bfor hutchig wr somwhat highr tha cotrol valu. C-144 5,18E+03 0, ,02 Hatchig startd about 3-4 days arlir tha that i th cotrol ad was mor prologd i tim.numbr did bfor hutchig wr somwhat highr tha cotrol valu. C-144 5,90E+04 0,001 0,234 Ngativ chags i th rd blood forlarva (1-7 days old). Coctratios rythrocyts wr 2-2,5 tims lowr tha that i th cotrol. MT MT MT MB Mashva & Sukalskaya (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) EPIC Dlivrabl 5. ANX B. 18

19 A41-4 A41-5 A41-6 A41-7 Fish Fish Fish Fish Salmo salar. Salmo Salmo salar. Salmo Salmo salar. Salmo Salmo salar. Salmo Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro. Exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro up to 234 days. C-144 5,18E+03 0, ,02 Ngativ chags i th rd blood forlarva (1-7 days old). Coctratios rythrocyts wr 2-2,5 tims lowr tha that i th cotrol. C-144 5,90E+04 0,001 0,234 Ngativ cytogtic chags i th rd blood forlarva (1-7 days old). Larg umbr abrrat mitoss was foud. Also picosis, lisis, vacuolisatio ucla ad cytoplasm wr dtctd i rd blood clls. C-144 5,18E+03 0, ,02 Ngativ cytogtic chags i th rd blood forlarva (1-7 days old). Larg umbr abrrat mitoss was foud. Also picosis, lisis, vacuolisatio ucla ad cytoplasm wr dtctd i rd blood clls. C-144 5,90E+04 0,001 0,234 Th amout primary sx clls i mbryos ( days gg dvlopmt) was tims highr tha that i th cotrol. Thr wr o abormal mbryos at activity coctratios lowr tha 10(-6) Ci/L MB CG CG CG Kasatkia t.al. (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) EPIC Dlivrabl 5. ANX B. 19

20 A41-8 A41-9 A41-10 A42-1 Fish Fish Fish Fish Salmo salar. Salmo Salmo salar. Salmo Salmo salar. Salmo Salmo salar. Salmo Exprimt, artificial icubatio ro up to 234 days. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. C-144 5,18E+03 0, ,02 Th amout primary sx slls i mbryos ( days gg dvlopmt) was tims highr tha that i th cotrol. Thr wr o abormal mbryos at activity coctratios lowr tha 10(-6) Ci/L C-144 5,90E+04 0,001 0,234 Ngativ chags i th thyroid glad ad hypophysis xposd forlarva, which ar typical for radiatio ractio: dstructiv chags i pitlia, picosis ucli, blokig hormo xtractio. C-144 5,18E+03 0, ,02 Ngativ chags i th thyroid glad ad hypophysis xposd forlarva, which ar typical for radiatio ractio: dstructiv chags i pitlia, picosis ucli, blokig hormo xtractio. Cs-137 2,20E+05 0,002 0,468 Ngativ chags i th rd blood forlarva (1-7 days old). Coctratios rythrocyts wr 2-2,5 tims lowr tha that i th cotrol. CG CG CG MB Kasatkia t.al. (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) EPIC Dlivrabl 5. ANX B. 20

21 A42-2 A42-3 A42-4 A42-5 Fish Fish Fish Fish Salmo salar. Salmo Salmo salar. Salmo Salmo salar. Salmo Salmo salar. Salmo Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro up to 234 days. Cs-137 1,85E+04 1,7E-04 0,039 Ngativ chags i th rd blood forlarva (1-7 days old). Coctratios rythrocyts wr 2-2,5 tims lowr tha that i th cotrol. Cs-137 2,20E+05 0,002 0,468 Ngativ cytogtic chags i th rd blood forlarva (1-7 days old). Larg umbr abrrat mitoss was foud. Also picosis, lisis, vacuolisatio ucla ad cytoplasm wr dtctd i rd blood clls. Cs-137 1,85E+04 1,7E-04 0,039 Ngativ cytogtic chags i th rd blood forlarva (1-7 days old). Larg umbr abrrat mitoss was foud. Also picosis, lisis, vacuolisatio ucla ad cytoplasm wr dtctd i rd blood clls. Cs-137 2,20E+05 0,002 0,468 Th amout primary sx slls i mbryos ( days gg dvlopmt) was tims highr tha that i th cotrol. MB CG CG CG Kasatkia t.al. (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) A42- Fish Salmo salar. Exprimt, Cs-137 1,85E+04 1,7E-04 0,039 Th amout primary sx slls i CG Kasatkia t.al. EPIC Dlivrabl 5. ANX B. 21

22 6 Salmo artificial icubatio ro up to 234 A42-7 A42-8 A43-1 A43-2 Fish Fish Fish Fish Salmo salar. Salmo Salmo salar. Salmo Carassius carassius Goldfish. Carassius carassius Goldfish. days. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro.exposur 234 days, forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio ro i radiouclid solutio from stag 8-16 blastomrs (N=40-50). Exprimt, artificial icubatio mbryos ( days gg dvlopmt) was tims highr tha that i th cotrol. Cs-137 2,20E+05 0,002 0,468 Ngativ chags i th thyroid glad ad hypophysis xposd forlarva, which ar typical for radiatio ractio: dstructiv chags i pitlia, picosis ucli, blokig hormo xtractio. Cs-137 1,85E+04 1,7E-04 () C-14 7,4E+05-7,3E+06 5,1E-04-5,1E-03 C-14 7,40E+04 0, ,039 Ngativ chags i th thyroid glad ad hypophysis xposd forlarva, which ar typical for radiatio ractio: dstructiv chags i pitlia, picosis ucli, blokig hormo xtractio. Mortality th goldfish ro was 2 tims highr (18-20%) tha i th cotrol (6,6%), p=0.5 Mortality th goldfish ro did ot diffr from th cotrol CG CG (1973) Kasatkia t.al. (1973) Kasatkia t.al. (1973) MT Fdorova (1964) Fdorova (1964) EPIC Dlivrabl 5. ANX B. 22

23 A44 A45 A46 A47 Fish Fish Fish Fish Rutilus rutilus Roach. Alburus alburus Blak. Acria crua Ruff. Tica tica Tch ro i radiouclid solutio from stag 8-16 blastomrs (N=40-50). Exprimt, artificial icubatio ro i radiouclid solutio from stag 8-16 blastomrs (N=40-45). Exprimt, artificial icubatio ro i radiouclid solutio from stag 8-16 blastomrs (N=47-57). Exprimt, artificial icubatio ro i radiouclid solutio from stag 8-16 blastomrs (N=65-74). Exprimt, artificial icubatio C-14 7,4E+04-7,3E+06 C-14 7,4E+04-7,3E+06 C-14 7,4E+04-7,3E+06 Sr ,7E+06 5,1E-05-5,1E-03 5,1E-05-5,1E-03 5,1E-05-5,1E-03 4,7E-07-4,7E-02 (xtral, Mortality roach ro was tims highr (33-47%) tha i th cotrol (17%) Mortality blak ro did ot diffr from cotrol Mortality ruff ro did ot diffr from cotrol Th prctag th hatchd was about th sam as i th cotrol. MT Fdorova (1964) Fdorova (1964) Fdorova (1964) Timva t.al. (1971) EPIC Dlivrabl 5. ANX B. 23

24 A48 Fish A49-1 Fish A49-2 Fish A50-1 Mollu sc A50-2 Mollu sc A51-1 Fish A51-2 Fish A51-3 Fish Prca fluviatilis Prch Pik Pik Limaa stagalis mollusk Limaa stagalis mollusk Pik. Pik. Pik. ro *) Exprimt, Sr ,7E-07 - artificial 3,7E+06 4,7E-02 icubatio (*, ro Exprimt, artificial icubatio ro Exprimt, artificial icubatio ro Exprimt, artificial icubatio ro Exprimt, artificial icubatio ro Exprimt, artificial icubatio ro, 7 days Exprimt, artificial icubatio ro, 7 days. Forlarva was kpt i cotamiatd watr. Exprimt, artificial icubatio Sr ,7E+06 Sr ,7E+06 xtral) 4,7E-07-4,7E-02 (*, xtral) 4,7E-03-4,7E-02 (*, xtral) Sr-90 1,85E+07 0,24 (xtral, rcostru ctio) Sr-90 3,70E+08 4,7 (*, xtral) Sr-90 3,7E+06-3E+07 0,047-0,37 (*, xtral) Sr-90 3,00E+07 0,37 (*, xtral) Sr-90 7,40E+07 0,94 (*, xtral) Th prctag th hatchd was about th sam as i th cotrol. Th prctag th hatchd was about th sam as i th cotrol. Numbr damagd aophass ad tlophass at th stag lat blastula was statistically highr tha that i th cotrol. Prctag hatchd bcam cosidrably lowr tha i th cotrol All mollu mbryos did durig 1 day Th prct did did ot diffr statistically from cotrol. Abormal larva comprisd 53% hatchd larva. Th abormalitis ld to th dath larva o th 15-22th day aftr th hatchig All hatchd forlarva had srious abormalitis CG MT MT MT MT Timva t.al. (1971) Timva t.al. (1971) Timva t.al. (1971) Timva t.al. (1971) Timva t.al. (1971) Pitkya & Shvdov (1971) Pitkya & Shvdov (1971) Pitkya & Shvdov (1971) EPIC Dlivrabl 5. ANX B. 24

25 A52 Fish Esox lucius(pik) A53 A54 Zoopl Zoopl A55-1 Bth os A55-2 Bth os A56-1 Fish Cyclops (Coppoda) Cladocra (Daphia logispia, Moia brachiata, Criodaphi a affiis, tc. I total, 6 spcis) Ostracoda Ostracoda Salmo salar. Atlatic salmo. ro, 7 days. Forlarva was kpt i cotamiatd watr. Kiv Rsrvoir th Dipr Rivr, fild study Exprimt, acut xposur Exprimt, acut xposur Exprimt, acut xposur Exprimt, acut xposur Exprimt, artificial icubatio Cs Extral gammaxposur Extral gammaxposur Extral gammaxposur Extral gammaxposur Extral gammaxposur (0,7-5,8)E-06 (itral), 3 typs abormalitis goads wr obsrvd: asymmtry goads (34.1%), ro rsorptio (12.5%), watr i goads (2.5%). Asymmtry goads was obsrvd i all pik gratios bor aftr th Chrobyl accidt. 2 Rproductio xposd cyclops was about 4 tims lowr tha i th cotrol. Liftim adult s did ot chagd. 20 Exposd s had highr fcudity comparig with th cotrol. 2,5-10 Exposd s had highr fcudity (up to 200%) comparig with th cotrol. 20 Exposd s had lowr fcudity comparig with th cotrol. 3,14 This dos causd 50% mortality mbryos (LD50) xposd at 1st day gg's dvlopmt Polyakova, 2001 Oako, 1973 STIM Oako, 1973 STIM Dolgushia, Oako, 1973 Dolgushia, Oako, 1973 MT Gorodilov, 1971 EPIC Dlivrabl 5. ANX B. 25

26 A56-2 Fish A57-1 Fish A57-2 Fish A58-1 Fish Salmo salar. Atlatic salmo. Corgous pld. Pld Corgous pld. Pld Salmo iridus Gairdrii. ro, 110 days. Acut xposur at o dvlopmt priods.stag "8-clls" mbrio Exprimt, artificial icubatio ro, 110 days. Acut xposur at days gg's dvlopmt Exprimt, artificial icubatio ro, 75 days.5 C. Acut xposur at 2-6 days dvlopmt. Exprimt, artificial icubatio ro, 75 days.5 C. Acut xposur at days dvlopmt. Exprimt, artificial icubatio Extral gammaxposur Extral gammaxposur Extral gammaxposur Extral gammaxposur 8 This dos causd 50% mortality mbryos (LD50) xposd at days gg's dvlopmt 5 This dos causd 100% mortality mbryos (LD) xposd at 2-6 days gg's dvlopmt 5 This dos causd 40% mortality mbryos (LD40) xposd at10-16 days gg's dvlopmt 5 This dos causd 100% mortality mbryos (LD) xposd at 3rd day gg's dvlopmt MT Gorodilov, 1971 MT Gorodilov, 1971 MT Gorodilov, 1971 MT Gorodilov, 1971 EPIC Dlivrabl 5. ANX B. 26

27 A58-2 Fish A59-1 Zoopl A59-2 Zoopl Raibow trout Salmo iridus Gairdrii. Raibow trout Cladocra (Daphia ) Cladocra (Daphia ) A60-1 Fish Tilapia mossambica (small aquarium fish) A60-2 Fish Tilapia mossambica ro. 5 C. Acut xposur at 3rd day dvlopmt. Exprimt, artificial icubatio ro, 75 days.5 C. Acut xposur at 11th day dvlopmt. Exprimt, sigl additio radiouclid i aquatic systm Exprimt, sigl additio radiouclid i aquatic systm Aquarium xprimt, chroic xposur durig th whol liftim, 550 days Aquarium xprimt, chroic xposur durig th whol liftim, 550 days Extral gammaxposur Ru-106 0,0003 Ci/l Ru-106 5E-03 Ci/l 0,2 (xtral, ) 3,3 (xtral, ) 5 This dos causd 60% mortality mbryos (LD40) xposd at 11th day gg's dvlopmt MT Gorodilov, 1971 No ffct o mortality Daphia Guskova t al., 1973 Dcras i survival Daphia by 15-20% Sr-90 3, ,00E-06 8,50E th day xprimt - both cotrol ad xposd fish wr rady for spawig. No ffct xposd fish had ormal goads. Sr-90 3,70E ,00E-04 0,1 213-th day xprimt: cotrol fish spcim wr rady for spawig. Exposd fish: goads mals wr smallr i mass tha i th cotrol, sprmatogsis was somwhat rducd. Fmals had ormal goads. MT Guskova t al., 1973 Shkhaova, 1983; Shkhaova, 1983 EPIC Dlivrabl 5. ANX B. 27

28 A60-3 Fish Tilapia mossambica A61-1 Fish Tilapia mossambica A61-2 Fish Tilapia mossambica A61-3 Fish Tilapia mossambica A62-1 Fish Tilapia mossambica Aquarium xprimt, chroic xposur durig th whol liftim, 550 days Aquarium xprimt, chroic xposur durig th whol liftim, 550 days Aquarium xprimt, chroic xposur durig th whol liftim, 550 days Aquarium xprimt, chroic xposur durig th whol liftim, 550 days Aquarium xprimt, chroic xposur Sr-90 3,70E (3-5)E-02 from 8 to 10 Sr-90 3, ,00E-06 (3-4)E-03 (rcostru ctio) Sr-90 3,70E ,00E-04 0,2-0,3 (rcostru ctio) Sr-90 3,70E (3-5)E- 02; 0,1 (goads) from 8 to th day xprimt: cotrol fish was rady for spawig. Exposd fish: 100% mals wr stril (N=120). Goads wr vitrous smitraspart bars or filamtous traspart bars. 30 % fmals had udrdvlopd ovaris. I total 80 % fmals had aomalis ovaris. Wak stimulatig ffct o frtility (tsts: % imprgatd ro ad th umbr ormal larva pr o fmal) Obsrvatios wr mad durig th whol rproductig priod fish Productio fish -120% cotrol. Rductio i th umbr ormal larva producd aftr spawig up to 80 % th cotrol (calculatd pr o fmal). Aftr spawigs fmals did arlir tha i th cotrol. Obsrvatios wr mad durig th whol rproductig priod fish Complt supprssio rproductio. Mals wr stril. O imprgatio xprimtal fmals by cotrol mals th producd larva did durig 160 days. Sr-90 1,48E+05 0,05 (y) 2 (y) Pathological dtrioratio ysight. Effct was obsrvd at doss to ys gratr tha 2 Gy Edma crystalli ls ad rtia th y, dystrophic dgratio th crystalli Shkhaova, 1983 MB Voroia, 1973,1974; rviwd i Shkhaova,1983 Voroia, 1973,1974; rviwd i Shkhaova,1983 (p.127); dtail dscriptio s i Abstract 4_6 Voroia, 1973,1974; rviwd i Shkhaova,1983 (p.127); dtail dscriptio s i Abstract 4_6 Shkhaova,1983, p.131. EPIC Dlivrabl 5. ANX B. 28

29 A63-1 Fish Tilapia mossambica A63-2 Fish Tilapia mossambica A64-1 Fish Tilapia mossambica A64-2 Fish Tilapia mossambica A65-1 Fish Cyprius carpio. Carp ( 1 yar old) Aquarium xprimt, chroic xposur, 800 days Aquarium xprimt, chroic xposur, 800 days Aquarium xprimt, chroic xposur, 180 days Aquarium xprimt, chroic xposur 180 days Aquarium xprimt, chroic xposur from Sr-90 up to 270 days. Aalyss blood compots o 15th, 30th,90th,180t Sr-90 (3,7-7,4)E+02 Sr-90 (3,7-7,4)E+04 substac, disturbac th structur photorcptors. 5,60E+04 4,00E-04 0,4 Liftim xposd fish was shortr tha i th cotrol. Survivd at th d xprimt (800 days) wr 54% xposd fish (cotrol - 71%) Sr-90 5,55E ,00E (body), 70 (bos), prlimia ry rcostru ctio (3-5)E-02 (5-9) (rcostru ctio Sr-90 3,70E ,00E-04 0,1(rco structio) 90 Sr 1,85E+03 1,85E+05 (bos, quilibriu m) 3,3E-05 (kidy) lss tha 0,3-0,4 Liftim xposd fish was shortr tha i th cotrol. Survivd at th d xprimt (800 days) wr 33% xposd fish (cotrol - 71%). Icrasig mortality o xprimtal ifctio with parasits: % spcims did i th xprimt ad % i th cotrol. Th tdcy for a icrasd mortality o xprimtal ifctio with parasits. Diffrcs from th cotrol wr statistically isigificat. Coctratios rd blood compots did ot diffr statistically from cotrol. MT Orlov, 1973, 1974; rviwd i Shkhaova,1983 MT Orlov, 1973, 1974; rviwd i Shkhaova,1983 MT Orlov, 1973, 1974; rviwd i Shkhaova,1983 (p.132); MT Orlov, 1973, 1974; rviwd i Shkhaova,1983 (p.132) Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 EPIC Dlivrabl 5. ANX B. 29

30 A65-2 Fish Cyprius carpio. Carp ( 1 yar old) A66-1 Fish Cyprius carpio. Carp ( 1 yar old) A66-2 Fish Cyprius carpio. Carp ( 1 yar old) h ad 270th days. Aquarium xprimt, chroic xposur from Sr-90 up to 270 days. Aalyss blood compots o 15th, 30th,90th,180t h ad 270th days. Aquarium xprimt, chroic xposur from Sr-90 up to 270 days. Aalyss blood compots o 15th, 30th,90th,180t h ad 270th days. Aquarium xprimt, chroic xposur from Sr-90 up to 270 days. Aalyss blood 90 Sr 3,70E+04 3,7E+06 (bos, quilibriu m) 90 Sr 1,85E+03 1,85E+05 (bos, quilibriu m) 90 Sr 3,70E+04 3,7E+06 (bos, quilibriu m) 2,7E-03 (kidy) 3,3E-05 (kidy) 2,7E-03 (kidy) lss tha 5,3 doss highr tha 0,04 doss highr tha 0,7 Coctratios rd blood compots did ot diffr statistically from cotrol.som abormalitis i rythrocyts wr foud. Coctratios lucocyts bcam ststistically lowr tha i th cotrol. Phas chags i lucocyts coctratio with tim wr dtctd. Coctratios lucocyts wr cosidrably lowr (about 50%) tha i th cotrol. Ivrsio i lymphocyt/graulocut/moocyt proportio MB MB MB Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 Shlifr & Shkhaova, 1977, 1980; Shkhaova, Orlov, Shlifr (1978); rviwd i Shkhaova,1983 EPIC Dlivrabl 5. ANX B. 30

31 A67-1 Fish A67-2 Fish Pik Pik A67-3 Fish Pik compots o 15th, 30th,90th,180t h ad 270th days. Watr body cotamiatd with radiouclids, Southr Urals, Russia. Fish wr obtaid from pik dwllig th cotamiatd watr body. Artificial icubatio ro i a)cla watr; b) cotamiatd watr. Artificial icubatio ro pik i watr cotamiatd with Sr-90 ad Cs-137. Watr body cotamiatd with radiouclids, Sr- 90+Cs- 137 Sr- 90+Cs- 137 Sr- 90+Cs- 137 Sr-90 (3,7E+03) +Cs-137 (3,7E+02) Sr-90 (3,7E+03) +Cs-137 (3,7E+02) Sr-90 (3,7E+03) +Cs-137 (3,7E+02) 0,0033 (bos); 0,0027(go ads) part fish 0,0033 (bos); 0,0027(go ads) part fish 0,0033 (bos); 0,0027(go ads) 1,2 Gy/yar (bos), 1Gy/pr yar (goads) xposur part fish 1,2 Gy/yar (bos), 1Gy/pr yar (goads) xposur part fish 1,2 Gy/yar (bos), 1Gy/pr Th umbr abormal forlarva from xposd fmal piks was cosidrably highr (30%) tha i th cotrol (1%). Th sam ffct was obsrvd i kpig i ithr cla or or cotaiatd watr. No diffrc with th cotrol i amout dath (14-18%) ad abormalitis forlarva (1%). No ffct growth pik. Growth rat was charactrizd as good comparig with th cotrol Pitkya (1978) Pitkya (1978) Pitkya (1978) EPIC Dlivrabl 5. ANX B. 31

32 A68-1 Bact ria plakt o A68-2 Bact ria plakt o A68-3 Bact ria plakt o Southr Urals, Russia. Fish wr obtaid from pik dwllig th cotamiatd watr body. Artificial icubatio ro i a)cla watr; b) cotamiatd watr. Exprimt, xtral gamma xposur, xprimt duratio - 79 day. Tst: th umbr bactia. Exprimt, xtral gamma xposur, xprimt duratio - 79 day. Tst: th umbr bactia. Exprimt, xtral gamma xposur, part fish 1,5 Gy/sco d 1,5 Gy/sco d 1,5 Gy/sco d yar (goads) xposur part fish Th umbr bactria was th miimal i th cotrol (396 thousad clls pr ml) i 4 hours aftr xposur for vry dos. Icras th umbr bactria i compariso with th cotrol for all variats doss i 1 day aftr xposur. Th umbr bactria was th maximum udr th dos i 7500 Gy (1870 thousad clls pr ml). Icras th umbr bactria i compariso with th cotrol for th doss 2500 Gy (4259 thousad clls pr ml)ad 7500 Gy (2990 thousad STIM STIM STIM Raziulyt,1973 Raziulyt,1973 Raziulyt,1973 EPIC Dlivrabl 5. ANX B. 32

33 A68-4 Bact ria plakt o A69 Phyto plakt o A70-1 Phyto plakt o A70-2 Phyto plakt 13 spcis alga i plic biocosis Scdsmu s quadricauda Pdiastrus boryaus xprimt duratio - 79 day. Tst: th umbr bactia. Exprimt, xtral gamma xposur, xprimt duratio - 79 day. Tst: th umbr bactia. Exprimt, xtral gamma xposur, xprimt duratio - 79 day. Tst: th umbr alga. Exprimt with pur culturs alga, xtral gamma xposur, xprimt duratio - 15 days. Tst: th biomass alga. Exprimt with pur 1,5 Gy/sco d 1,5 Gy/sco d 1,5 Gy/sco d 1,5 Gy/sco clls pr ml) i 29 days aftr xposur. No diffrc i th umbr bactria from cotrol for th rst doss. Th umbr bactria was th miimal i th cotrol i 79 days aftr xposur for vry dos. Th umbr bactria was th maximum udr th dos i 7500 Gy (1424 thousad clls pr ml). Th umbr alga was th miimal i th cotrol i 79 days aftr xposur. for vry dos (xcpt dos Gy). Th umbr alga was th maximum udr th dos i 2500 Gy (47654 thousad clls pr ml) Dras th biomass alga for dos 400 Gy by factor 2 i compariso with th cotrol Dcras th biomass alga for dos 400 Gy by factor 6 i STIM STIM MT MT Raziulyt,1973 Raziulyt,1973 Raziulyt,1973 Raziulyt,1973 EPIC Dlivrabl 5. ANX B. 33

34 A70-3 Phyto plakt o A71-1 Zoopl A71-2 Zoopl o culturs alga, xtral gamma xposur, xprimt duratio - 15 days. Tst: th Phormidium uciatum 8 spcis aimals i plic biocosis 8 spcis aimals i plic biocosis biomass alga. Exprimt with pur culturs alga, xtral gamma xposur, xprimt duratio - 15 days. Tst: th biomass alga. Exprimt, xtral gamma xposur, xprimt duratio - 79 day. Tst: th umbr pl aimals. Exprimt, xtral gamma xposur, xprimt duratio - 79 d 1,5 Gy/sco d 1,5 Gy/sco d 1,5 Gy/sco d compariso with th cotrol No discrpacy for th biomass alga udr xposur i compariso with th cotrol. Th biomass alga was 387 mg/l udr dos 400 Gy, ad 354 mg/l i th cotrol Dcras th umbr pl aimals i compariso with th cotrol (2516 1/L) for all variats doss i 1 day aftr xposur. Plic aimal wr obsrvd oly for dos 200 Gy ad cotrol i 29 ad 79 days aftr xposur. Dath plic aimals udr th doss 500 Gy ad highr. MT MT Raziulyt,1973 Raziulyt,1973 Raziulyt,1973 EPIC Dlivrabl 5. ANX B. 34

35 A71-3 Zoopl A72-1 Mollu sc A72-2 Mollu sc A72-3 Mollu sc 8 spcis aimals i plic biocosis Lymaa stagalis L. Pod sail Lymaa stagalis L. Pod sail Lymaa stagalis L. day. Tst: th umbr pl aimals. Exprimt, xtral gamma xposur, xprimt duratio - 79 day. Tst: th umbr pl aimals. Exprimt, xtral gamma xposur to mbryo pod sails from Ural laks. Tst: survival mbryos. Exprimt, xtral gamma xposur to mbryo pod sails from Ural laks. Tst: survival mbryos. Exprimt, xtral 1,5 Gy/sco d 0,195 Gy/miut 0,195 Gy/miut 0,195 Gy/miut 200 Dcras th umbr spcis pl aimals from 8 to 3 i 79 days aftr xposur. 6 Dcras th survival mbryos pod sails aftr gamma-xposur 6 Gy i compariso with th cotrol. Th survival irradiatd mbryos was 27,9% (27,7-28,1%) (N=497), ad 97,6% (97,3-97,9%) (N=641) i th cotrol (Lak Bol'shoy Tatkul', July). 6 Dcras th survival mbryos pod sails aftr gamma-xposur 6 Gy i compariso with th cotrol. Th survival irradiatd mbryos was 72,8% (67-791%) (N=554), ad 97,5% (96,9-98,1%) (N=874) i th cotrol (Lak Bol'sho Miassovo', July). 6 Dcras th survival mbryos pod sails aftr gamma-xposur 6 MT MT MT MT Raziulyt,1973 Famlis,1973 Famlis,1973 Famlis,1973 EPIC Dlivrabl 5. ANX B. 35

36 A72-4 Mollu sc A72-5 Mollu sc A73-1 Mollu sc Pod sail Lymaa stagalis L. Pod sail Lymaa stagalis L. Pod sail Patiopct yssosis Scallop gamma xposur to mbryo pod sails from Ural laks. Tst: survival mbryos. Exprimt, xtral gamma xposur to mbryo pod sails from Ural laks. Tst: survival mbryos. Exprimt, xtral gamma xposur to mbryo pod sails from Ural laks. Tst: survival mbryos. Exprimt, xtral gamma xposur to scallops at th ag 1 ad 2 yars. Tst: ability to 0,195 Gy/miut 0,195 Gy/miut 0,1 Gy/sco d Gy i compariso with th cotrol. Th survival irradiatd mbryos was 31,1% (26-36,2%) (N=984), ad 94,5% (91,6-97,4%) (N=828) i th cotrol (Lak Bol'shoy Tatkul', August). 6 Dcras th survival mbryos pod sails aftr gamma-xposur 6 Gy i compariso with th cotrol. Th survival irradiatd mbryos was 47,8% (42-53,6%) (N=716), ad 99,3% (99,1-99,5%) (N=705) i th cotrol (Lak Bol'sho Miassovo', August). 6 Dcras th survival mbryos pod sails aftr gamma-xposur 6 Gy i compariso with th cotrol. Th survival irradiatd mbryos was 49,9% (45-54,8%) (N=1424), ad 99,3% (99,1-99,5%) (N=705) i th cotrol (Lak Bol'sho Miassovo', August). 7,5 Dstructiv chags i goads th scallops at th ag 1 ad 2 yars: coagulatio karyoplasm, xtoliatio cytoplasm from xtral cll mmbra, apparac larg vacuols, ad dfcts cytosklto. Ths chags wr obsrvd i 2-4 days aftr xposur. Th aimals wr MT MT Famlis,1973 Famlis,1973 Kulpaov,1989 EPIC Dlivrabl 5. ANX B. 36

37 A73-2 Mollu sc Patiopct yssosis Scallop A74-1 Fish Misgurus fossilis Loach A74-2 Fish Misgurus fossilis Loach A75-1 Zoopl A75-2 Zoopl Daphia maga Daphia Daphia maga rproductio. Exprimt, xtral gamma xposur to scallops at th ag 1 ad 2 yars. Tst: ability to rproductio. Exprimt, xtral gamma xposur to mals loach. Tst: dvlopmt larva fish. Exprimt, xtral gamma xposur to mals loach. Tst: dvlopmt larva fish. Exprimt, xposur to Daphia i 130 days. Tst: rproductio Exprimt, xposur to U mg/l (12,3 Bq/l) U mg/l (12,3 0,1 Gy/sco d 0,77 Gy/miut 0,77 Gy/miut No data 0,00074 ay No data 0,00074 ay sxually matur i 1 yar aftr xposur 7,5 Gy. 15 Dstructiv chags i goads th scallops at th ag 1 ad 2 yars: coagulatio karyoplasm, xtoliatio cytoplasm from xtral cll mmbra, apparac larg vacuols, ad dfcts cytosklto. Ths chags wr obsrvd i 2-4 days aftr xposur. Th aimals wr stril i 1 yar aftr xposur 15 Gy. 0,5-10 Icras dad ad abormal larva with icras dos to body fish. Dlay larva mrgs was obsrvd o th dos 10 Gy. 2 Dcras dad ad abormal larva by factor 2 udr th xposur 2 Gy to had mals i compariso cotrol. 0,022 Gy/moth 0,022 Gy/moth Dcras th frtility rat (th umbr bor aimals from 10 fmals for 30 days) o 21% i compariso with th cotrol. Icras th itrval btw moultigs o 1 day (for last gratio STIM Kulpaov,1989 Nchavskii,1989 Nchavskii,1989 Tlitchko,1958; Strogaov,1959 Tlitchko,1958; Strogaov,1959 EPIC Dlivrabl 5. ANX B. 37

38 A75-3 Zoopl A75-4 zoopl A75-5 Zoopl A75-6 Zoopl A75-7 Zoopl A75-8 Zoopl Daphia Daphia maga Daphia Daphia maga Daphia Daphia maga Daphia Daphia maga Daphia Daphia maga Daphia Daphia maga Daphia Daphia i 130 days. Tst: rproductio Exprimt, xposur to Daphia i 130 days. Tst: rproductio Exprimt, xposur to Daphia i 130 days. Tst: rproductio Exprimt, xposur to Daphia i 130 days. Tst: rproductio Exprimt, xposur to Daphia i 130 days. Tst: rproductio Exprimt, xposur to Daphia i 130 days. Tst: rproductio Exprimt, xposur to Daphia i 130 days. Tst: Bq/l) aimals) i compariso with th cotrol. Th itrval btw moultigs was 5 days i xprimt, ad 4 days i th cotrol. U mg/l (12,3 Bq/l) U mg/l (12,3 Bq/l) U-238 0,5 mg/l (6,15 Bq/l) U-238 0,5 mg/l (6,15 Bq/l) U-238 0,5 mg/l (6,15 Bq/l) U-238 0,5 mg/l (6,15 Bq/l) No data 0,00074 ay No data 0,00074 ay No data 0,00037 ay No data 0,00037 ay No data 0,00037 ay No data 0,00037 ay 0,022 Gy/moth 0,022 Gy/moth 0,011 Gy/moth 0,011 Gy/moth 0,011 Gy/moth 0,011 Gy/moth Icras th priod pubscc itrval o 5 days (for last gratio aimals) i compariso with th cotrol. Th priod pubscc was 14 days for irradiatd aimals, ad 9 days i th cotrol. Dcras th littr from 1 fmal by factor 3,4 (for last gratio aimals) i compariso with th cotrol. Icras th frtility rat (th umbr bor aimals from 10 fmalts for 30 days) o 13% i compariso with th cotrol. Dcras th itrval btw moultigs o 1 day (for last gratio aimals) i compariso with th cotrol. Th itrval btw moultigs was 3 days i xprimt, ad 4 days i th cotrol. Dcras th priod pubscc itrval o 2 days (for last gratio aimals) i compariso with th cotrol. Th priod pubscc was 7 days for irradiatd aimals, ad 9 days i th cotrol. Dcras th littr from 1 fmal by factor 2,2 (for last gratio aimals) i compariso with th cotrol. STIM STIM STIM Tlitchko,1958; Strogaov,1959 Tlitchko,1958; Strogaov,1959 Tlitchko,1958; Strogaov,1959 Tlitchko,1958; Strogaov,1959 Tlitchko,1958; Strogaov,1959 Tlitchko,1958; Strogaov,1959 EPIC Dlivrabl 5. ANX B. 38