ANALYTICAL METHODS FOR SIMULTANEOUS ESTIMATION OF OFLOXACIN AND ORNIDAZOLE ABSTRACT

Transcription

1 J.Bio.Iov7 (5), pp: , 2018 ISSN (Elctroic) By t al., ANALYTICAL METHODS FOR SIMULTANEOUS ESTIMATION OF OFLOXACIN AND ORNIDAZOLE Jai by*, Kavitha M.P & K. Krishakumar Dpartmt of pharmacutical aalysis, St Jams Collg of pharmacutical scic St Jams Hospital Trust Pharmacutical Rsarch ctr (DSIR Rcogizd), Chalakudy, Krala ABSTRACT For th stimatio of ofloxaci ad oridazol may aalytical mthods ar rportd icludig spctrophotomtric mthod ad HPLC mthod.th prst study icluds with simultaous quatio mthod, absorptio ratio mthod, drivativ spctrophotomtric mthod ad mthod basd o Q aalysis usig uv visibl spctroscopy. Ad i th cas of HPLC mthod C18 rvrs phas colum hav b usd xcpt o mthod th phyl colum hav b usd.a comparativ HPLC mthod is usd i th studis ad compard th rsult obtaid.c18 rvrs phas coluym is usd i almost all studis.ad hr th studis xplaid about th simpl simultaous quatio spctrophotomtric mthod as wll as th most sophisticatd HPLC mthod. 1 Kywords : Ofloxaci,Oridazol,HPLC,UV- spctrophotomtry. No: of Tabls: 2 No: of Rfrcs: Sptmbr Editio Iovativ Associatio

2 J.Bio.Iov7 (5), pp: , 2018 ISSN (Elctroic) By t al., INTRODUCTION Ofloxaci chmically is (±)-9-fluoro-2,3- dihydro-3-mthyl-10-(4-mthyl-1-pipraziyl)- 7-oxo-7H-pyrido [1,2,3-d]-1,4-bzoxazi- 6-carboxylic acid. It is a broad spctrum atibactrial agt, blogig to th group of fluoroquiolos. It is usd i th tratmt of uriary tract ifctio, cojuctivitis, goorrhoa, rspiratory tract ifctio ad ski ifctio. Ofloxaci is admiistrd alog with cfixim or oridazol for th tratmt of ifctio. Svral aalytical tchiqus hav b usd for th dtrmiatio of ofloxaci i dosag forms as wll as i biological fluids such as spctrophotomtry ad HPLC mthods 1.May aalytical mthods has b rportd for th simultaous stimatio of ofloxaci ad oridazol icludig spctrophotomtric mthods ad hplc mthods. A rviw of various aalytical mthods availabl for th stimatio of ofloxaci ad oridazol is prstd hr 2. Spctrophotomtric mthods May aalytical mthods hav b rportd which ar commoly usd for multicompot sampl aalysis by spctrophotomtry Ths mthods icluds simultaous quatio mthod, absorptioratio mthod drivativ spctrophotomtric mthod ad mthod basd o Q -aalysis. Two drivativs spctrophotomtric mthod hav b rportd for th simultaous stimatio of ofloxaci ad oridazol. Both th mthods usd th zro crossig wavlgth i first drivativ spctra.i o mthod th zro prst wavlgth choos ar 322m ad 260m for ofloxaci ad oridazol rspctivly. I aothr mthod 78m ad 293.6m hav b usd for th stimatio of ofloxaci ad oridazol rspctivly. I both th mthod 0.1M hcl is usd th liarity of th all tob abov 0.5mg /ml to 10 mg/ml ofloxaci ad 2mg- 30 mg/ml for oridazol.i cas of absorptio ratio mthod ofloxaci is stimatd from th ratio of 266m ad 287m whr as th oridazol was stimatd from th ratio of absorbac at m usig 0.1 N Hcl as solvt. May simultaous quatio has b rportd i o mthod phosphat PH 6.8 is usd as a solvt ad th wavlgth. Chos for aalysis ar 488m ad 319m.th liarity was foud tob 5-20mcg/ml for ofloxaci ad mcg/ml for oridazol i th scod mthod th aalysis is carrid out by mthaol as solvt ad th wavlgth choos ar 294.6m ad 310.8m. I this mthod th liarity was foud tob 2 mcg /ml 10mcg/ml for ofloxaci ad 5 mcg/ml 25 mcg/ml for oridazol.th third mthod is basd o of masurmtof absorbac at 287m- 319m i phosphat, ph 6.8 or ph 7.4. th stimatio is also i 0.1m hydrochloric acid ad masurig th absorbac at 494 m ad 277 m.th coctratio rag is 1 8 mg/ml ofloxaci 4-24 mg/ml for oridazol. A mthod basd o Q aalysis is also b rportd th mthod is basd o masurig th absorbac at 286.1m th 2018 Sptmbr Editio Iovativ Associatio

3 J.Bio.Iov7 (5), pp: , 2018 ISSN (Elctroic) By t al., ƛmax of ofloxaci, is 319.2m th ƛmax of oridazol ad 494m th isobstic poit. Th absorbac valus at ths wavlgth ar usd for to aalysis for th of th coctratio of th ach drug. 3 Th simultaous quatio mthod has b rportd for th stimatio of ofloxaci ad oridazol i dissolutio mdia (0.01N hydrochloric acid).this is basd o th masurmt of absorbac of 293.4m ad 319.6m.Udr similar coditio multicompot aalysis of softcor of th istrumtatio that also b usd for stimatio.th liarity was foud tob 2-12 mcg/ml for ofloxaci ad 5 13 mcg/ml for oridazol.a similar mthod basd o simultaous quatio ad th multicompot aalysis mad of th istrumt is also rportd.sic thr is solubility problm for ofloxaci ad oridazol i watr hydrotropic solubilisatio tchiqus has b usd to dissolv ths drugs i aqua s mdium usig sodium bzoat as hydrotropic ragt.this will avoid th us of orgaic solvt th rsultig solutio is stimatd by simultaous quatio mthod, Q aalysis or absorptio ratio mthod First drivativ 2. Absorpti o ratio 1. First drivativ-323m ad 260m for ofloxaci ad oridazol 2. Absorptio ratio mthod m for ofloxaci ad 271 m of oridazol 0.1N Hcl mcg/mlforofl oxaci mcg/ml for oridazol First drivativ Q aalysis Simultaous quatio mthod Simultaous quatio mthod Wavlgth is 278m for ofloxaci 293.6m for oridazol 286.2m,319.2m,294m(isobstic poit) 288m ad 319m for ofloxaci ad oridazol 294.6mfor ofloxaci ad for oridazol 0.1N Hcl Distill d watr Phosp hat ph-6.8 mtha ol mcg/ml for ofloxaci 2-30mcg for oridazol mcg for ofloxaci 1-13mcg for oridazol mcg for ofloxaci mcg for oridazol mcg/ml ofloxaci ad 5-25 mcg/ml oridazol A comparativ accout of th rportd spctrophotomtric mthod of uv spctroscopyi th tabl Sptmbr Editio Iovativ Associatio

4 J.Bio.Iov7 (5), pp: , 2018 ISSN (Elctroic) By t al., May HPLC mthods has b rportd for th simultaous stimatio of ofloxaci ad oridazol.i almost all th mthod C18 rvrs phas colum hav b usd xcpt o mthod th phyl colum hav b usd..as wll as th coctratio of solutio.th ru tim has rprstd by rttio tim of scod pak varis from 4.4 miuts to 10.4 miuts.shortst ru tim is achivd with actoitril ad phosphat. 5 Similarly i almost all th mthods actoitril is usd as th mobil phas with phosphat xcpt i o mthod ammoium actat is usd as A comparativ accout of HPLC mthod is giv i tabl 2. mthod colum Mobil phas Buffr Tmprat ur 1 C18 colum 2 Zorbas clips c18 3 Erk c 18 sscoloum 4 C18 colum Watr,actoitr il actoitril Trithyl ami, actoitril,buf fr Actoitril, mthaol 0.3Ndis odium hydrog phospha t Potassiu m dihydro g phospha t i 1000ml watr 0.025M phospha t 25 dgr clcius 45 dgr clcius Flow rat 1.0ml/ mi 1.5 ml/mi Rtti o tim Oridaz ol 2.6 miut Rttio tim Ofloxaci 4.8miut Thortic al plats Ofloxaci /5000- mor tha1.4 oridazol /8000- mor tha Ofloxaci Oridazo l Ofloxaci Oridazo l Ofloxaci Oridazo rf rc Sptmbr Editio Iovativ Associatio

5 J.Bio.Iov7 (5), pp: , 2018 ISSN (Elctroic) By t al., 5 Kromasil C18 colum 6 RP Zorbas thrmal colum C18 colum RP C18 colum Thrmo hyprsil phyl colum Actoitril phosphat Actoitril,mi xd phosphat Actoitril ad Actoitril phosphat ad actoitril,actoitr il Phospha t Phospha t Ambit ambit ambit ambit ambit ambit 0.9ml/ mi l Ofloxaci Oridazo l Ofloxaci Oridazo l Ofloxaci oridazo l Ofloxaci Oridaz ol Itrsil ODS C18 colum ODS c18 colum Ammoium actat, actoitril 1ml trithyl ami, 1000ml doubl distilld watr Ammoi um actat actoitr il ambit ambit mi 7.50mi Ofloxaci Oridaz ol CONCLUSION: A larg umbr of aalytical mthods has b rportd for th simultaous stimatio of ofloxaci ad oridazol ragig from simpl simultaous quatio spctrophotomtric mthod to most sophisticatd HPLC mthod Sptmbr Editio Iovativ Associatio

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