An Introduction To The Use of Laboratory Animals at TCD. José Manuel Sánchez Morgado
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1 An Introduction To The Use of Laboratory Animals at TCD José Manuel Sánchez Morgado
2 Designated Veterinarian DV available 24/7/365 Jose M. Sanchez Morgado Responsible for ensuring good animal health and welfare for all animals on site Examination of sick and dead animals Advice on animal matters
3 Who are you?
4 1. TCD position on Animal Research 2. Comparative Medicine 3. S.I. No 543 of Laboratory Animal Allergy
5 The Use of Animals in Biomedical Research The 3R s 1. Replacement 2. Reduction 3. Refinement
6 The Principles of Humane Experimental Technique Russell and Burch, 1959 W.M.S. Russell and R.L. Burch, 1959
7 3Rs Definition Replacement Replacement means the substitution for conscious living higher animals of insentient material Reduction Reduction means reduction in the numbers of animals used to obtain information of a given amount and precision Refinement Refinement means any decrease in the incidence or severity of inhumane procedures applied to those animals which still have to be used
8 Animal Welfare at TCD A culture of care 24 hour veterinarian cover Happy animals
9 Comparative Medicine Animal Husbandry Administration & License control Veterinary Services
10 Comparative Medicine Contact details in webpage!
11 Units The Smurfit Institute of Genetics Animal Facility Lloyd Building, Trinity College Institute of Neuroscience (TCIN) Animal Facility Trinity Biomedical Sciences Institute (TBSI) Animal Facility
12 Barrier system
13 Animal Husbandry Species: Rodents Rabbits Fish (Pigs)
14 Animal Husbandry ACWO: What does it mean?
15 Animal Husbandry Access to units Occupational Health Laavanya Parthasarathi ext Handling Euthanasia HPRA Mandatory Training for Individual Authorisations
16 Euthanasia Let s work with examples!
17 Euthanasia 1. Group of 15 mice fish rat embryos
18 Euthanasia Directive EU Annex IV Methods other than those listed may be used: a) on unconscious animals, providing the animal does not regain consciousness before death; b) on animals used in agricultural research (Annex I to Council Regulation (EC) No 1099/2009 of 24 September 2009 on the protection of animals at the time of killing)
19 Euthanasia The killing of animals shall be completed by one of the following methods: a) confirmation of permanent cessation of the circulation; b) destruction of the brain; c) dislocation of the neck; d) exsanguination; or e) confirmation of the onset of rigor mortis.
20 Microbiology Regular screening of animal units (infectious organisms) FELASA recommendations. IMPACT testing Histopathology support Necropsies to sick animals Pathology interpretation on tissues
21 Administration & Licence Control Animal Ordering Authorisation applications etc. Contact : Dr Laavanya Parthasarathi (parthasl@tcd.ie, X4554)
22 S.I. No. 543 of 2012 Three authorisation system Establishment (Trinity College Dublin) Project Individual Trinity College Dublin is a licensed establishment: AE19136
23 Individual Authorisation Assume primary responsibility for the welfare of Protected Animals Authorised Individuals must work under a Project authorisation Authorised Individuals must ensure they UNDERSTAND Seeks advice from the DV and ACWO
24 HPRA Inspector
25 Animal Research Ethics Committee (AREC)
26 Laboratory Animal Allergy Clinical signs Conjunctivitis and Rhinitis Skin rashes Asthma Allergens
27 Laboratory Animal Allergy Personal Protective Equipment
28 Laboratory Animal Allergy Increased risk pre- existing allergies genetic predisposition Smokers Occupational Health Department Lung function test College Health Centre (X1591/X1556)
29 What next? Occupational health College Health Centre Handling and Euthanasia training ACWO Access to the animal facility Laavanya Parthasarathi Individual Authorisations Laavanya Parthasarathi
30 GOOD LUCK!
31 WHY ANIMALS ARE NEEDED IN RESEARCH
32 Thank You!
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