LABORATORY ANIMAL SCIENCE FOR RESEARCHERS PLANNING ANIMAL EXPERIMENTS AND PERSONS PERFORMING PROCEDURES BASIC GENERAL MODULE (LAS 301)
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1 LABORATORY ANIMAL SCIENCE FOR RESEARCHERS PLANNING ANIMAL EXPERIMENTS AND PERSONS PERFORMING PROCEDURES BASIC GENERAL MODULE (LAS 301) Aurora Brønstad, Chief veterinarian, The Animal Facilities - University of Bergen Aurora Brønstad - Veterinary surgeon, Oslo 1995 PhD Physiology at University of Bergen 2004 Head of Animal facilities at University of Bergen ( XX) Member of Scand-LAS, ESLAV, LASA, AALAS AALAS - FELASA working group on harm-benefit analysis of animal studies 1
2 Administrative questions: Asta and Atle THE LABORATORY ANIMAL FACILITY Core Facility Leader Aurora Brønstad DVM-PhD 2
3 The Laboratory Animal Facility The Laboratory Animal Facility is a open core facility at UoB and provides for service to researchers in experimental medicine in animal. The laboratory animal facility offers: Modern well equipped facilities Operation rooms for large animas Operation rooms for small animals Animal imaging equipment in collaboration with MIC MRI, PET, Optical imaging, Ultrasound P3 facility for experiments in contagious diseases. Breeding unit for gene modified rodents Controlled and monitored environmental conditions Microbiological surveillance program Education in Laboratory Animal Science Competence in traditional laboratory animals (rodents and pigs) 3
4 The Laboratory Animal Facility The Animal Facility have a good program for animal welfare, quality and scientific validity and have therefore been awarded full accreditation from AAALAC international This accreditation provides researchers assurance in a global community as well as it demonstrates accountability. WHY DO YOU HAVE TO TAKE THIS COURSE??? Personnel and competence According to 24 and 25 in the regulation on animal experimentation, personnel working with animals at breeders, suppliers and users shall have the minimum competence described in Appendix E before they start to plan/design experiments, care for or euthanize animals. Mattilsynet emphasizes that it is each individual researcher's responsibility to ensure that your CV is up to date in relation to the requirements for continuous updating and training in laboratory animal science. Norwegian Regulation on the Use of Animals in Research 4
5 Personnel and competence Persons responsible for planning experiments (function B) shall be adequately educated in the scientific field they work within and have specific competence in the relevant species biology, physiology and behavioral needs of the species in question. Persons performing procedure (Function A), animal caretakers (function C) or killing animals (Function D) shall be supervised until they demonstrate that they master the necessary skills. Education and training framework adapted to Directive 2010/63 published by the European Commission rochure%202%20education_trainin g-en.pdf 5
6 Means Aims Regulations and ethics Characteristics of the animals Influence of external factors Planning experiments and research design Managing the human factor Laboratory Animal Science Contributing to the humane use of animals in biomedical research and to the collection of informative unbiased and reproducible data. Laws, regulations, guidelines, 3R, ethics BRØNSTAD 2009 Anatomy, physiology, genetics, biochemistry, microbiology Environmental conditions, Interventions, Anesthesia, surgery etc.. Quality control systems, SOP, GLP, statistics, experimental design Communication Cooperation, Coordination and Contracts 6
7 Qualified to plan and design animal experiments Qualified to start practical training under guidance Course AIM LAS is a multidisciplinary branch of science aiming to promote humane use of animals in research and collection of informative, reliable and reproducible data LAS Includes laws, regulations, ethics, factors of animals and external factors that influence experiments, literature search, planning and design of experiments as well as cooperation between different actors involved in the experiment. Persons planning animal experiments (group B) must demonstrate knowledge 1 of these factors and also demonstrate that they are able to apply them in a new contexts 2 Persons performing procedures on animals (group A) will at University of Bergen be taught the same theoretical curriculum as group B and in addition they must demonstrate that they master the practical skills 3 for the procedures that they actually are going to perform in animals. 1: Assessed by multiple choice test, 2: group work solving a case 3: Practical test LAS courses at University of Bergen Persons designing procedures and projects (function B) Exam Las 302/303 LAS 302: mammals (2 days) LAS 303: fish (2 days) Analgesia and Anaesthesia Anaesthesia, Environmental conditions, OR Rodent biology, housing and care water quality, Welfare and behaviour Large animals Biology of commonly used species Gene modified animals and phenotyping Nutrition, Handling Animal qualities and health monitoring Stress and diseases Exam and Group-work LAS 301 Part 1 - (2 days) LAS 301 organized twice per year) and includes Introduction to LAS, Legislation and administrative procedures, Ethical application, General anaesthesia, euthanasia, Safety and health hazards in the animal facility, Humane endpoints, Basic handling techniques (video - interactive training) alternatives to use of animal, planning and design of animal experiments, 3R, GLP SOP, Ethics, Zoonoses Preparation for lectures and demonstrations MCQ 2 MCQ 1 7
8 Qualified to plan and design animal experiments Qualified to start practical training under guidance Function A performing procedures Practical training Practical training Practical training Exam Las 302/303 LAS 302: mammals (2 days) Analgesia and Anesthesia Rodent biology, housing and care OR Large animals Gene modified animals and phenotyping Animal qualities and health monitoring LAS 303: fish (2 days) Anesthesia, Environmental conditions, water quality, Welfare and behavior Biology of commonly used species Nutrition, Handling Stress and diseases Exam and group work LAS 301 Part 1 - (2 days) LAS 301 organized twice per year) and includes Introduction to LAS, Legislation and administrative procedures, Ethical application, General anesthesia, euthanasia, Safety and health hazards in the animal facility, Humane endpoints, Basic handling techniques (video - interactive training) ): alternatives to use of animal, planning and design of animal experiments, 3R Teaching methods E-lectures Traditional lectures Discussions in class Demonstrations Quizzes and tests Group work 1-1 or small group practical training (group A) 8
9 Lectures: e-lectures and traditional lectures 9
10 MCQ Readiness assurance test Curriculum Compendium in Laboratory Animal Science See Dyreavdelingens web site for further information Norwegian: English: Both the book and compendia are available from Studia Handouts are available from Dyreavdelingens web page 10
11 HANDOUTS Group work Distributed the last day of the lectures Will be organized though edmodo Goto group code is gb6vxh 11
12 Practical training Requirements for technical skills apply only to those who will perform procedures on animals. Persons planning lab animal projects, but do not themselves perform procedures, must know these procedures and what impact they have on the animals, but they do not need to demonstrate that they have mastered them in practice. The aim of practical training is to get the knowledge and skills necessary to handle the animal with as little discomfort for the animal as possible, and thereby both prevent trauma for the animal and unwanted variation in research results. The Alternative lab Free of charge! Contact for an appointment 12
13 Lunch / You can have lunch in the cantina in the Hospital (3 th floor) or in the Hospital lobby BBB building Lunch is not included Coffee/tea served by Dyreavdelingen in the morning and after lunch REMEMBER TO SIGN THE PARTICIPATION LIST!!! If you do not sign every day your participation will not be registered 13
14 REMEMBER TO CHECK THAT PERSONAL INFO IS CORRECT IN THE CIRCULATED LIST If not we cannot reach you, you may loose the exam Visiting the animal facility Project startup meeting Contact us for a visit post@viv.uib.no 14
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