Capnocytophaga leadbetteri 1

Transcription

1 Capnocytophaga leadbetteri 1 1) 2) 1) 1) 1) 1) 1) 1) 4) 3) 3) 3) 3) 4) 2) 3) 1) 2) 3) 4) Capnocytophaga 16S rrna Capnocytophaga ochracea C. leadbetteri 16S rrna C. leadbetteri Key words: Capnocytophaga leadbetteri 16S rrna Capnocytophaga 1) C. canimorsus, C. suptigena, C. ochracea 2) 4) 5) 7) Capnocytophaga C. leadbetteri cefotiam 2 g/

2 cefpirome 2 g/ 3 18 CT cefpirome 3g/ clindamycin 1,800 mg/ 4 2 VII 4 5 cefmetazole 2 g/ ) G F BTB 1. Capnocytophga ( ) ( ) ( ) ranging, 2 3 HK HK Capnocytophaga 1 Capnocytophaga RapID TM ANA II Remel ID NH20 Capnocytophaga sp. 2, 3 Capnocytophaga DNA 16S rrna PCR (BLAST) 50

3 Capnocytophaga leadbetteri RapID TMANA II Test code Reactive ingredient Result URE Urea ( ) BLTS p-nitrophenyl-b,9-disaccharide ( ) aara p-nitrophenyl-a,a-arabinoside ( ) ONPG p-nitrophenyl-b,9-galactoside ( ) aglu p-nitrophenyl-a,9-galactoside ( ) bglu p-nitrophenyl-b,9-galactoside ( ) agal p-nitrophenyl-a,9-galactoside ( ) afuc p-nitrophenyl-a,a-fucoside ( ) NAG p-nitrophenyl-n-acetyl-b,9-gulucosaminde ( ) PO4 p-nitrophenylphosphate ( ) LGY Leucyl-glycine-b-naphthylamide ( ) GLY Glycine-b-naphthylamide ( ) PRO Proline-b-naphthylamide ( ) PAL Phenylalanine-b-naphthylamide ( ) ARG Arginine-b-naphthylamide ( ) SER Serine-b-naphthylamide ( ) PYR Pyrrolidonyl-b-naphthylamide ( ) IND Tryptophane ( ) Value total Microcode: Capnocytophga sp. 3. ID NH-20 Test code Reactive ingredient Result 2. 16S rrna ALA ( ) PHO ( ) NIT ( ) URE ( ) ODC ( ) IND ( ) GLS ( ) PRO ( ) gga g- ( ) GLU ( ) MLT ( ) FRU ( ) MAS ( ) MAN ( ) TRE ( ) SUC ( ) LAC ( ) XYL ( ) ONP b ( ) NIR ( ) bla b ( ) Value total Microcode: Capnocytophga sp. C. ochracea (ATCC T ) (Accession No. L14635) MIC (mg/ml) MIC (mg/ml) PCG 0.06 IPM 0.12 ABPC 0.12 AMPC/C 0.12 PIPC 0.5 CLDM 0.06 CZX 0.5 MINO 0.12 CMZ 0.5 SPFX 0.25 FMOX 0.5 CP 4 2 C. leadbetteri 16S rrna 16S rrna C. leadbetteri (CCUG T ) DNA (Accession No. DQ ) 99.4 (1428/1436) 2 C. leadbetteri DP23 (MIC) )

4 174 Streptococcus anginosus 2 19 S. anginosus Prevotella melaninogenica Staphylococcus aureus (methicillin-sensitive; MSSA) S. anginosus P. melaninogenica, MSSA P. buccae, Neisseria subflava Capnocytophaga 1982 Leadbetter ) C. leadbetteri 9 C. canimorsus C. cynodegmi 2 C. gingivalis, C. granulosa, C. haemolytica, C. ochracea, C. sputigena C. leadbetteri C. leadbetteri Capnocytophaga 62 multilocus enzyme electrophoresis (MLEE), 16S rrna 5 8) Capnocytophaga 3 Capnocytophaga C. canimorsus C. cynodegmi 2 C. canimorsus 9), 10) 11) 12) C. canimorsus Capnocytophaga C. haemolitica 13) C. gingivaris 14) 15) C. sputigena 2) C. gingivaris, C. ohracea, C. sputigena Capnocytophaga C. ohracea 16), 17) C. leadbetteri Capnocytophaga 2 4 Capnocytophaga C. leadbetteri Capnocytophaga 9 16S rrna 2 C. leadbetteri 52

5 Capnocytophaga leadbetteri C. leadbetteri C. leadbetteri S. anginosus, P. melaninogenica C. leadbetteri Capnocytophaga 1) Morita, M., T. Yamamoto, T. Watanabe Identification by biotinylated DNA probes of Capnocytophaga species isolate from supragingival calculus. J. Dent. Res. 70: ) 1993 Capnocytophaga sputigena 67: ) 1990 Capnocytophaga ochracea 1 64: ) Capnocytophaga canimorsus 1 15: ) Ndon, J. A Capnocytophaga canimorsus septicemia caused by a dog bite in a hairy cell leukemia patient. J. Clin. Micribiol. 30: ) Khawari, A. A., J. W. Myers, D. A. Ferguson, Jr., et al Sepsis and meningitis due to Capnocytophaga cynodegmi after splenectomy. Clin. Infect. Dis. 40: ) Wald, K., A. Martinez, S. Moll, Capnocytophaga canimorsus infection with fulminant sepsis in an asplenic patient: Diagnosis by review of peripheral blood smear. Am. J. Hematol. 83: ) Frandsen, E. V., K. Poulsen, E. Kononen, et al Diversity of Capnocytophaga species in children and Capnocytophaga leadbetteri sp. nov. and Capnodyutophaga genospecies AHN Int. J. Syst. Evol. Microbiol. 58: ) Kleijnen-Grebien, B., S. Boorsma, F. S., Stals, et al Fatal case of sepsis with Capnocytophaga canimorsus after a minor dog bite, Ned. Tijdschr. Geneeskd. 152: ) Völl, K., G. Haase, H. Fritz, et al Septic shock with purpura fulminans after a dog bite. Dtsch. Med. Wochenschr. 132: ) Rosenman, J. R., J. K., Reynolds, et al Capnocytophaga canimorsus meningitis in a newborn: An avoidable infection. Pediatr. Infect. Dis. J. 22: ) Risi, Jr., G. F., C. A. Spangler, Capnocytophaga canimorsus meningitis after routine myelography: A sentinel event identifies multiple opportunities for improvement of standard practices in radiology. Am. J. Infect. Control 34: ) Gutierrez-Martin, M. A., O. A. Araji, J. M. Barquero, et al Aortic valve endocarditis by Capnocytophaga haemolytica. Ann. Thorac. Surg. 84: ) Mantadakis, E., V. Danilatou, A. Christidou, et al Capnocytophaga gingivalis bacteremia detected only on quantitative blood cultures in a child with leukemia. Pediatr. Infect. Dis. J. 22: ) Rodgers, G. L., J. E. Mortensen, D. P. Goldsmith Pyogenic arthritis caused by Capnocytophaga gingivalis in an immunocompetent three-year-old male. J. Clin. Rheumatol. 7: ) Wang, H. K., Y. C. Chen, L. J. Teng, et al Brain abscess associated with multidrugresistant Capnocytophaga ochracea infection. J. Clin. Microbiol. 45: ) Desai, S. S., R. A. Harrison, M. D. Murphy Capnocytophaga ochracea causing severe sepsis and purpura fulminans in an immunocompetent patient. J. Infect. 54:

6 176 A Case of Pyothorax Caused by a New Species Capnocytophaga leadbetteri in an Elderly Patient after Surgery of the Gastroesophageal Junction Cancer Nobuko Sato, 1) Kiyofumi Ohkusu, 2) Yuko Sasaki, 1) Makoto Katumi, 1) Maho Kato, 1) Yuko Abe, 1) Machiko Endo, 1) Mituaki Nagasawa, 1) Chikashi Sibata, 4) Hisakazu Yano, 3) Natuo Yamamoto, 3) Hiroyuki Kunishima, 3) Yoichi Hirakata, 3) Iwao Sasaki, 4) Takayuki Ezaki, 2) Mituo Kaku 3) 1) Division of Clinical Laboratory, Department of Laboratory Medicine, Tohoku University Hospital 2) Department of Microbiology, Gifu University of Graduate School of Medicine 3) Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine 4) Division of Biological Regulation & Oncology, Department of Surgery We report a patient su#ered from acute pleural e#usion and pyothorax, 5 days after surgery of gastroesophageal junction cancer. The patient is a 59-year-old man, who had a sense of postprandial blocking of the chest, in August He was clinically diagnosed with gastroesophageal junction cancer. On March 12, 2007, his malignant tumors were totally removed by resection of lower esophagus and all stomach after adjuvant chemotherapy. During his recovery, a minor complication of pyothorax occurred on March 15, The biochemical profile of the isolate produced by a RapID ANA II identification system disclosed Capnocytophaga species. Further identification of the isolate was performed by 16S rrna gene sequencing. The sequence data obtained were then compared with known 16S rrna gene sequences in the GenBank database by BLAST search. However, the sequence of the isolate failed to provide an unequivocal identification, as the closest 16S rrna gene maximum identity was 95.9 (Capnocytophaga ochracea; GenBank accession no. L14635). After several months of the search, repeated the BLAST analysis revealed that the sequence corresponded to the new species Capnocytophaga leadbetteri with 99.4 identity. To our knowledge, this is the first report of an infection in an elderly patient associated with this microorganism. 54