The Use of Molecular Tools in Ecological Studies
|
|
|
- Clara Lewis
- 5 years ago
- Views:
Transcription
1 International Workshop on Applied Ecology and Human Dimensions in Biological Conservation Biota FAPESP The Use of Molecular Tools in Ecological Studies Eduardo Eizirik Faculdade de Biociências, PUCRS, Brasil Instituto Pró-Carnívoros, Brasil Laboratory of Genomic Diversity, NIH, USA
2 Molecular Markers in Conservation Biology. Inventories of biodiversity - Systematics, species delimitation - Species identification - DNA barcoding 2. Geographic distributions, community composition, habitat association 3. Monitoring of species and populations 4. Phylogeography, population structure and demographic history - Definition of units for conservation 5. Behavioral ecology (social structure, dispersal patterns) 6. Forensics 7. Adaptation and natural selection (including at the genomic level)
3 Different Markers for Different Tasks. Deep phylogenetics 2. Shallow phylogenetics and phylogeography 3. Species identification 4. Population genetics 5. Parentage/kinship analyses 6. Individual identification 7. Sexing 8. Pathogen identification 9. Analysis of genes involved in immune response 0. Analysis of genes involved in potentially adaptive phenotypes
4 Development of molecular tools for carnivore species identification from faecal DNA
5 Development of molecular tools for carnivore species identification from faecal DNA bpon56 (fezes Panthera onca em álcool) bpon55 (fezes de Panthera onca em DET) bpon306 (fezes de Panthera onca) bpon307 (fezes de Panthera onca) bpon56 (fezes Panthera onca - 98 mg) bpon32 (sangue de Panthera onca) bpon56 (fezes de Panthera onca em DET) P3-6 (pele de Panthera onca) bpon305 (fezes de Panthera onca) P3-2 (pêlos de Panthera onca) bpon56 (fezes de Panthera onca - 48 mg) bpon24 (sangue de Panthera onca) P3- (pêlos de Panthera onca) F2-636 (fezes de felino grande) 99 Puma concolor (sangue) L.V.(fezes de felino grande) F57-C (fezes de felino grande) F2-635 (fezes de felino grande) Felis catus 0.02 Haag et al. 2009
6 Identification of carnivores using short DNA segments Goal: global standardized saystem for carnivore identification. Application in ecological, biogeographic and forensic studies. Ongoing tests in multiple field sites in South America Brazil (RS, ES, MS, MT, TO, MG,DF), Argentina. P.B. Chaves et al., in prep.
7 Identification of carnivores using short DNA segments Ongoing surveys and ecological studies
8 Identification of carnivores using short DNA segments Testing and optimization of protocols for African carnivores: Partnership with CCF, Namibia.
9 25 car70r Reverse Complement 2 Car78 2 bfca57 30 bfca bfca52 bfca54 46 Car73 bfca Lse bfca55 bfca58 bfca02 bfca56 NC Felis catus Fma Fma 67Lse Fni serval 3 serval Fni4 AY AY598470S0 Lynx canadensis AH SEG AY598470S Lynx canadensis Car69 Cca2 Cca CarCaracal Car49 car88r Reverse Complement car93r Reverse Complement car54copy car47 car76r Reverse Complement Aju NC Acinonyx jubatus Car48 AY Acinonyx jubatus car87r Reverse Complement 0 AF Acinonyx jubatus car89r Reverse Complement 7 car94r Reverse Complement car2r Reverse Complement Car Ppa car70r Reverse Complement 2 Car78 2 bfca57 30 bfca53 bfca bfca54 46 Car73 bfca Car8ccfAndBrazil CarLeop 88 Rme Ple85 Ple Hja 88 car84copy Reverse Complement lionf car52 Phe Hpa Pcr Hbr Cfe NC Neofelis nebulosa Hhy40 Ccr34 Ccr236 Pli Vci babi Ial2 NC Halichoerus grypus Zca Oro AY Hydrurga leptonyx AY Hydrurga leptonyx NC Hydrurga leptonyx 8 9 bfca bfca58 39 bfca02 bfca56 NC Felis catus Fma Man68 AY Ommatophoca rossii AY Ommatophoca rossii Bas Bal 97 Fma 7 AM7898. Spilogale putorius complete 0 bcar24 29 AB Meles meles anakuma AB Martes melampus AB Enhydra lutris 0 Ist Ssu 0 Car AY AY598506S0 Alopex lagopus AH SEG AY598506S Alopex lagopus CarBatEaredFox 45 Band2and5 carwilddog DQ Canis lupus isolate fro... 6 AY AY598494S0 Canis lupus DQ Canis familiaris isolate... AH SEG AY598494S Canis lupus 38 DQ Canis lupus isolate 2 fro... DQ Canis lupus isolate fro... DQ Canis familiaris isolate... AY Canis familiaris isolate AY Canis familiaris AY Canis familiaris isolate... NC Canis lupus familiaris DQ Canis familiaris isolate... DQ Canis lupus isolate fro DQ Canis familiaris isolate... DQ Canis lupus isolate fro... NC Canis lupus 86 DQ Canis lupus isolate fro... car50copy 85 car85r Reverse Complement AY Canis familiaris isolate... NC Canis latrans DQ4805. Canis latrans isolate 2 f DQ Canis latrans isolate f... DQ Canis latrans isolate f... Car79 45 car23 copy 78 Car53 Car8 2 car74r Reverse Complement 30 Car7 car63r Reverse Complement 7 Car57 Car56 27 Car car9r Reverse Complement 2 Car82 car45r Reverse Complement Car9ccf Car64 Car68 2 Car5 Car80 car90r Reverse Complement Car43 Car83 Car77 car44copy car55copy 48 car62 7 car66 27 car65copy 6 car60copy Car75 IUCN Cat Specialist Group
10 car70r Reverse Complement 2 Car78 2 bfca57 Lse bfca53 bfca52 bfca54 Car73 bfca59 bfca55 bfca58 bfca02 bfca56 NC Felis catus Fma Fma 67Lse8 Fni serval 3 serval Fni4 AY AY598470S0 Lynx canadensis AH SEG AY598470S Lynx canadensis Car69 Cca2 Cca CarCaracal Car49 car88r Reverse Complement car93r Reverse Complement car54copy car47 car76r Reverse Complement Aju NC Acinonyx jubatus Car48 AY Acinonyx jubatus car87r Reverse Complement 0 AF Acinonyx jubatus car89r Reverse Complement 7 car94r Reverse Complement car2r Reverse Complement Car2 Ppa286 Car8ccfAndBrazil CarLeop 88 Rme Ple85 Ple Hja car84copy Reverse Complement lionf car52 Phe Hpa Pcr Hbr Cfe NC Neofelis nebulosa Hhy40 Ccr34 Ccr236 Pli Vci babi Ial2 NC Halichoerus grypus Man68 Zca Oro AY Hydrurga leptonyx AY Hydrurga leptonyx NC Hydrurga leptonyx AY Ommatophoca rossii AY Ommatophoca rossii Bas Bal AM7898. Spilogale putorius complete bcar24 AB Meles meles anakuma 6 Ist AB Martes melampus AB Enhydra lutris Ssu Car2 AY AY598506S0 Alopex lagopus AH SEG AY598506S Alopex lagopus CarBatEaredFox Band2and5 carwilddog DQ Canis lupus isolate fro... AY AY598494S0 Canis lupus DQ Canis familiaris isolate... AH SEG AY598494S Canis lupus Car69 Cca2 Cca Caracal 38 DQ Canis lupus isolate 2 fro... DQ Canis lupus isolate fro... DQ Canis familiaris isolate... AY Canis familiaris isolate AY Canis familiaris AY Canis familiaris isolate... NC Canis lupus familiaris DQ Canis familiaris isolate... DQ Canis lupus isolate fro DQ Canis familiaris isolate... DQ Canis lupus isolate fro... NC Canis lupus 86 DQ Canis lupus isolate fro... car50copy 85 car85r Reverse Complement AY Canis familiaris isolate... NC Canis latrans DQ4805. Canis latrans isolate 2 f DQ Canis latrans isolate f... DQ Canis latrans isolate f... Car79 45 car23 copy 78 Car53 Car8 2 car74r Reverse Complement 30 Car7 car63r Reverse Complement 7 Car57 Car56 27 Car car9r Reverse Complement 2 Car82 car45r Reverse Complement Car9ccf Car64 Car68 2 Car5 Car80 car90r Reverse Complement Car43 Car83 Car77 car44copy car55copy 48 car62 7 car66 27 car65copy 6 car60copy Car75
11 car70r Reverse Complement Car78 2 bfca57 Lse bfca53 bfca52 bfca54 Car73 bfca59 bfca55 bfca58 bfca02 bfca56 NC Felis catus Fma Fma 67Lse8 Fni serval 3 serval Fni4 AY AY598470S0 Lynx canadensis AH SEG AY598470S Lynx canadensis Car69 Cca2 Cca CarCaracal Car49 car88r Reverse Complement car93r Reverse Complement car54copy car47 car76r Reverse Complement Aju NC Acinonyx jubatus Car48 AY Acinonyx jubatus car87r Reverse Complement 0 AF Acinonyx jubatus car89r Reverse Complement 7 car94r Reverse Complement car2r Reverse Complement Car2 Ppa286 Car8ccfAndBrazil CarLeop 88 Rme Ple85 Ple Hja car84copy Reverse Complement lionf car52 Phe Hpa Pcr Hbr Cfe NC Neofelis nebulosa Hhy40 Ccr34 Ccr236 Pli Vci babi Ial2 NC Halichoerus grypus Man68 Zca Oro AY Hydrurga leptonyx AY Hydrurga leptonyx NC Hydrurga leptonyx AY Ommatophoca rossii AY Ommatophoca rossii Bas Bal AM7898. Spilogale putorius complete bcar24 AB Meles meles anakuma Ist AB Martes melampus AB Enhydra lutris Ssu Car2 AY AY598506S0 Alopex lagopus AH SEG AY598506S Alopex lagopus CarBatEaredFox Band2and carwilddog DQ Canis lupus isolate fro... AY AY598494S0 Canis lupus DQ Canis familiaris isolate... AH SEG AY598494S Canis lupus DQ Canis lupus isolate 2 fro... DQ Canis lupus isolate fro... DQ Canis familiaris isolate... AY Canis familiaris isolate... AY Canis familiaris AY Canis familiaris isolate... NC Canis lupus familiaris DQ Canis familiaris isolate... DQ Canis lupus isolate fro... DQ Canis familiaris isolate... DQ Canis lupus isolate fro... Car49 car88r Reverse Complement 97 car93r Reverse Complement 46 car54copy 50 car47 33 car76r Reverse Complement 4 Aju NC Acinonyx jubatus 23 Car48 AY Acinonyx jubatus car87r Reverse Complement 0 AF Acinonyx jubatus car89r Reverse Complement 7 car94r Reverse Complement NC Canis lupus 86 DQ Canis lupus isolate fro... car50copy 85 car85r Reverse Complement AY Canis familiaris isolate... NC Canis latrans DQ4805. Canis latrans isolate 2 f DQ Canis latrans isolate f... DQ Canis latrans isolate f... Car79 45 car23 copy 78 Car53 Car8 2 car74r Reverse Complement 30 Car7 car63r Reverse Complement 7 Car57 Car56 27 Car car9r Reverse Complement 2 Car82 car45r Reverse Complement Car9ccf Car64 Car68 2 Car5 Car80 car90r Reverse Complement Car43 Car83 Car77 car44copy car55copy 48 car62 7 car66 27 car65copy 6 car60copy Car75
12 car70r Reverse Complement Car78 2 bfca57 Lse bfca53 bfca52 bfca54 Car73 bfca59 bfca55 bfca58 bfca02 bfca56 NC Felis catus Fma Fma 67Lse8 Fni serval 3 serval Fni4 AY AY598470S0 Lynx canadensis AH SEG AY598470S Lynx canadensis Car69 Cca2 Cca CarCaracal Car49 car88r Reverse Complement car93r Reverse Complement car54copy car47 car76r Reverse Complement Aju NC Acinonyx jubatus Car48 AY Acinonyx jubatus car87r Reverse Complement 0 AF Acinonyx jubatus car89r Reverse Complement 7 car94r Reverse Complement car2r Reverse Complement Car2 Ppa286 Car8ccfAndBrazil CarLeop 88 Rme Ple85 Ple Hja car84copy Reverse Complement lionf car52 Phe Hpa Pcr Hbr Cfe NC Neofelis nebulosa Hhy40 Ccr34 Ccr236 Pli Vci babi Ial2 NC Halichoerus grypus Man68 Zca Oro AY Hydrurga leptonyx AY Hydrurga leptonyx NC Hydrurga leptonyx AY Ommatophoca rossii AY Ommatophoca rossii Bas Bal AM7898. Spilogale putorius complete bcar24 AB Meles meles anakuma 6 38 Ist AB Martes melampus AB Enhydra lutris Ssu Car2 AY AY598506S0 Alopex lagopus AH SEG AY598506S Alopex lagopus CarBatEaredFox Band2and5 28 carwilddog DQ Canis lupus isolate fro... AY AY598494S0 Canis lupus DQ Canis familiaris isolate... AH SEG AY598494S Canis lupus DQ Canis lupus isolate 2 fro... DQ Canis lupus isolate fro... DQ Canis familiaris isolate... AY Canis familiaris isolate... AY Canis familiaris AY Canis familiaris isolate... NC Canis lupus familiaris 9 99 car2r Reverse Complement 6 Car2 Ppa Car8 CarLeop Ple85 48 Ple53 88 car84 75 lionf DQ Canis familiaris isolate... DQ Canis lupus isolate fro DQ Canis familiaris isolate... DQ Canis lupus isolate fro... NC Canis lupus 86 DQ Canis lupus isolate fro... car50copy 85 car85r Reverse Complement AY Canis familiaris isolate... NC Canis latrans DQ4805. Canis latrans isolate 2 f DQ Canis latrans isolate f... DQ Canis latrans isolate f... Car79 45 car23 copy 78 Car53 Car8 2 car74r Reverse Complement 30 Car7 car63r Reverse Complement 7 Car57 Car56 27 Car car9r Reverse Complement 2 Car82 car45r Reverse Complement Car9ccf Car64 Car68 2 Car5 Car80 car90r Reverse Complement Car43 Car83 Car77 car44copy car55copy 48 car62 7 car66 27 car65copy 6 car60copy Car75
13 car70r Reverse Complement Car78 2 bfca57 Lse bfca53 bfca52 bfca54 Car73 bfca59 bfca55 bfca58 bfca02 bfca56 NC Felis catus Fma Fma 67Lse8 Fni serval 3 serval Fni4 AY AY598470S0 Lynx canadensis AH SEG AY598470S Lynx canadensis Car69 Cca2 Cca CarCaracal Car49 car88r Reverse Complement car93r Reverse Complement car54copy car47 car76r Reverse Complement Aju NC Acinonyx jubatus Car48 AY Acinonyx jubatus car87r Reverse Complement 0 AF Acinonyx jubatus car89r Reverse Complement 7 car94r Reverse Complement car2r Reverse Complement Car2 Ppa286 Car8ccfAndBrazil CarLeop Rme Ple85 Ple53 Hja car84copy Reverse Complement lionf car52 Phe Hpa Pcr Hbr Cfe NC Neofelis nebulosa Hhy40 Ccr34 Ccr236 Pli Vci babi Ial2 NC Halichoerus grypus Man68 Zca Oro AY Hydrurga leptonyx AY Hydrurga leptonyx NC Hydrurga leptonyx AY Ommatophoca rossii AY Ommatophoca rossii Bas Bal AM7898. Spilogale putorius complete bcar24 AB Meles meles anakuma Ist AB Martes melampus AB Enhydra lutris Ssu Car AY AY598506S0 Alopex lagopus AH SEG AY598506S Alopex lagopus CarBatEaredFox Band2and Car79 86 Car53 carwilddog DQ Canis lupus isolate fro... AY AY598494S0 Canis lupus DQ Canis familiaris isolate... AH SEG AY598494S Canis lupus DQ Canis lupus isolate 2 fro... DQ Canis lupus isolate fro... DQ Canis familiaris isolate... AY Canis familiaris isolate... AY Canis familiaris AY Canis familiaris isolate... NC Canis lupus familiaris DQ Canis familiaris isolate... DQ Canis lupus isolate fro... DQ Canis familiaris isolate... DQ Canis lupus isolate fro... NC Canis lupus DQ Canis lupus isolate fro... car50copy 85 car85r Reverse Complement AY Canis familiaris isolate... NC Canis latrans DQ4805. Canis latrans isolate 2 f... DQ Canis latrans isolate f... DQ Canis latrans isolate f... Car8 car23 copy car74r Reverse Complement Car7 car63r Reverse Complement Car57 Car Car56 car9r Reverse Complement 2 Car82 car45r Reverse Complement Car64 Car68 2 Car Car80 Car9ccf car90r Reverse Complement Car43 Car83 Car77 car44copy car55copy car62 car car65copy car60copy Car75 43 DQ Canis familiaris isolate... DQ Canis lupus isolate fro DQ Canis familiaris isolate... DQ Canis lupus isolate fro... NC Canis lupus 86 DQ Canis lupus isolate fro... car car85R AY Canis familiaris isolate... NC Canis latrans DQ4805. Canis latrans isolate 2 f DQ Canis latrans isolate f... DQ Canis latrans isolate f... Car79 car23 78 Car53 Car8 car74r Car7 car63r Car57 Car56 Car car9r Car82 car45r Car9ccf Car64 Car68 Car5 Car80 car90r Car43 Car83 Car77 car44copy car55copy car62 car66 car65copy car60copy Car75
14 Ongoing projects: phylogeny, phylogeography, population genetics and molecular ecology
15 Comparative phylogeography and demographic history of two South American procyonids: Nasua nasua and Procyon cancrivorus Mirian T. N. Tsuchiya-Jerep
16 N. nasua LBGM samples UCLA samples (Dr. Klaus Peter-Koepfli) Skin samples (MZUSP)
17 P. cancrivorus LBGM Samples Skin samples (MZUSP)
18 Data sets mtdna: 3 segments NADH Dehydrogenase subunit 5 (ND5) 750 bp Cytochrome b (Cytb) 40 bp Control region (D-loop) 300 bp Microsatellites Nasua nasua : development of a library and isolation of markers Procyon cancrivorus: heterologous transfer of markers from P. lotor.
19 Ln P(X D) Ln P(X D) Nasua nasua Procyon cancrivorus Number of clusters (K ) Number of clusters (K )
20 Maned wolf conservation biology Integrating ecology, genetics, health sciences and education programs
21 Maned wolf population structure on a macrogeographic scale Manoel Rodrigues
22 Toninha Halei Pierre Mama Roni Piumhi Laís Jimy Guga Feminha Christy Ulna Roman Chew Lindi? Amadeu Thay Gambá Taís Sofia Melissa Cerveja Mel Chorão Roger Paquito Salete? Katrina Jerônimo Júnior Machinho Vitória
23 Molecular ecology of the Neotropical otter (Lontra longicaudis) Cristine Trinca A C B
24 DNA ext. cont. PCR Control PCR control Molecular ecology of the Neotropical otter (Lontra longicaudis) Cristine Trinca * *
25 Using scat DNA to predict a jaguar s coat color MCR NH 2 Extra-cellular Intra-cellular L T L L E L 25 C D 2 S 90 Jaguar M 73 MCR - 5 S 7 Eizirik et al
26 Genotyping of the jaguar MCR deletion
27 Genotypes for the 5 bp deletion in the jaguar MCR gene n* Genotypes 5 / 5 5 / + + / + Wild-type color Melanistic *Geographic sampling: Mexico to southern Brazil; only unrelated animals were included. Fisher s Exact test: P<0.00
28 Using scat DNA to predict a jaguar s coat color. Expanded association study 2. Blind test with scat samples 3. Field test of scat samples
29
30 Conservation genetics of fragmented jaguar populations Modificado de Sanderson et al. (2002) e Zeller (2007)
31 Di Bitteti et al. 2003
32 Estimated number of individuals at each site: P. E. Morro do Diabo (SP): 9-5 (Cullen 2007) MS SP UE Porto Primavera (MS/SP): 0-20 (Crawshaw et al. 993) PR P. E. das Várzeas do Rio Ivinhema: 0 (Sana et al. 2006) Corredor verde (Brasil/Argentina/Paraguai): (Paviolo et al. 2008)
33 Sampling UE Porto Primavera (SP/MS) (-6) n = 23 P. E. do Morro do Diabo (SP) (7) n = 8 P.E. das Várzeas Rio Ivinhema (MS) (8-9) n = 0 Corredor verde (Brasil/Arg/Paraguai) (-2) n = 8
34 Measures of diversity at 3 microsatellite loci in the four jaguar populations of the UPAF investigated in this study. Sample size (N), observed number of alleles (A), allelic richness (AR), observed (Ho) and expected (He) heterozygosities. Green Corridor (n= 8) Morro do Diabo (n= 8) Ivinhema (n= 0) Porto Primavera (n= 23) Locus N A AR H o H e 2 N A AR H o H e 2 N A AR H o H e 2 N A AR H o H e 2 FCA FCA FCA FCA FCA F F F F F F FCA FCA Overall Allelic richness is calculated using minimum sample size of eight diploid individuals; 2 Unbiased gene diversity (corrected for sampling bias in FSTAT)
35 Relative frequencies Private alleles for each population: Morro do Diabo 0 Ivinhema 2 Porto Primavera 3 Corredor verde F Alleles Fig. 2 Histograms showing the distribution of the allele frequencies
36 Population structure: Identification of migrants and admixed individuals M M Corredor verde M.Diabo Ivinhema Porto Primavera AM
37 Genetic differentiation between populations: Table 4. Pairwise FST (above the diagonal) and RST values (below the diagonal) for the four jaguar populations of the UPAF. Green Corridor Morro do Diabo Ivinhema Porto Primavera Green Corridor *** 0.22*** 0.048*** Morro do Diabo 0.2** *** 0.073*** Ivinhema 0.08** *** Porto Primavera * Significant values *P < 0.05, **P < 0.0 and ***P < 0.00
38 Effective population size estimates and their approximate confidence limits for each UPAF jaguar population based on two different methods (LDNE and ONESAMP). LDNE ( P crit = 0.05) ONESAMP (Prior = 2 -) N e Confidence limits (95%) N e Confidence limits (95%) Green Corridor Morro do Diabo Ivinhema Porto Primavera
39 Field data: None of the radio-colared individuals has dispersed among these study sites during recent monitoring Cullen 2007 Jaguars significantly avoid the use of human-modified areas, but some individuals do show long-distance movement across the fragmented landscape (Cullen 2007)
40 Conclusions -Surprising result given the geographic proximity of the field sites (ca. 69 km to 500 km) and the dispersal ability of this species - Molecular evidence for recent gene flow - Recent isolation (30-40 yrs, ca. 6-8 generations) - Small effective size and restricted gene flow among areas - Comparative data from other species Strong impact of humanindiced genetic drift in remnant jaguar populations
41 Neotropical felids: Genus Leopardus
42 Distributions of L. tigrinus and L. geoffroyi? Leopardus tigrinus L. colocolo Leopardus geoffroyi
43 Rio Grande do Sul State, Brazil E. Eizirik et al. 2006
44
45 Habitat use of L. tigrinus and L. geoffroyi in RS state Argentina N Uruguay Atlantic Ocean Gramineous Steppe Park Steppe Arboreal Steppe Steppical Savannah Semidecidual Stational Forest Decidual Stational Forest Mixed Ombrophilous Forest Dense Ombrophilous Forest Pioneer Formations Ecological Tension Area miles
46 % of prey in diet Dietary analysis of L. tigrinus e L. geoffroyi using stomach contents of roadkilled animals Leopardus tigrinus Leopardus geoffroyi Akodon sp Calomys sp Delomys dorsalis Holochilus brasiliensis Mus musculus Oligorymozys sp Oryzomys sp Oxymycterus sp Rattus rattus Cavia sp Monodelphis sp Prey category Columbiformes Cuculiformes Gruiformes Passeriformes Tinamiformes Sauria Serpentes Anura
47 Dietary analysis of L. tigrinus e L. geoffroyi using stomach contents of roadkilled animals: DNA barcoding of prey items CE0 Puma yagouaroundi Ne306 CE02 Puma yagouaroundi Ne306 OPN57 Oligoryzomys nigripes OPN87 Oligoryzomys nigripes OPN55 Oligoryzomys nigripes JR332 Oligoryzomys flavescens JR20 Oligoryzomys flavescens JR209 Oligoryzomys flavescens JR202 Oligoryzomys flavescens OPN60 Oligoryzomys nigripes JR244 Oligoryzomys flavescens CE32 L. geoffroyi Lgeof08 CE39 L. geoffroyi NE32 CE47 L. tigrinus blti22 EU Oligoryzomys fulvescens PCE2 Deltamys kempi CE0 L. tigrinus NE32 CE20 L. tigrinus blti22 CE03 Leopardus geoffroyi Lge076 CE04 Leopardus geoffroyi Lge076 JR96 Sooretamys angouya 9790 Nectomys squamipes CE6 L. tigrinus blti CE63 L. tigrinus NE Juliomys pictipes 9667 Juliomys pictipes CE25 L. tigrinus blti079 CE65 L. tigrinus NE022 CE05 L. geoffroyi blge074 CE59 L. tigrinus blti069 JR 405 Calomys tener LBCE 5556 Calomys expulsus CRB 2732 Calomys expulsus LBCE 547 Calomys expulsus CE0 Leopardus geoffroyi Ne32 LBCE 548 Calomys expulsus CE66 L. tigrinus NE33 CE62 L. tigrinus NE022 JR395 Oxymycterus nasutus JR328 Akodon azarae JR329 Akodon azarae 9595 Akodon montensis PCE23 Oligoryzomys nigripes JR 407 Akodon montensis CE06 Leopardus geoffroyi Ne Akodon paranaensis 6963 Akodon paranaensis CE09 P. yagouaroundi NE34 JR4 Akodon paranaensis 0.02
48 Acknowledgements Sandro L. Bonatto Thales R. Freitas Francisco M. Salzano Larissa Oliveira Flávio H. Rodrigues Dênis Sana Laury Cullen Tadeu Oliveira Cibele Indrusiak Ronaldo G. Morato Fernando Azevedo Fernanda Michalski Steve O Brien Warren Johnson Robert Wayne Klaus Koepfli Agostinho Antunes Bill Murphy Mark Springer Rogério de Paula Guilherme Mourão Rita Bianchi Fabio Mazim Cladinara R. Sarturi Support: CNPq, CAPES, FAPERGS, NIH, NSF, FNMA, CESP, WCS, WWF, CVRD, PUCRS Tatiane C. Trigo Taiana Haag Cristine S. Trinca Manoel F. Rodrigues Alexsandra Schneider Ezequiel Fabiano Eunice M. Matte Paulo B. Chaves Mirian Tsuchiya Fernanda P. Valdez Henrique V. Figueiró Flávia P. Tirelli Carlos Benhur Kasper Renata Bornholdt Anelisie S. Santos Taiz L. Simão Livia Lehugeur Camila Jaeger Vanessa Dias
49 Distributions of L. tigrinus, L. geoffroyi e L. colocolo? Leopardus tigrinus L. colocolo Leopardus geoffroyi
50 Sampling for genetic analyses (mtdna e STRs) Trigo et al. (2008) Mol. Ecol
51 Bayesian analysis of microsatellite genotypes Trigo et al. (2008) Mol. Ecol
52 mtdna Control Region blti30 blge06 blti33 blti60 blti34 blti0 blge46 blge79 blti7 blge Lco02 blti47 blti68 blti30 blti29 blti74 blti89 blti blti93 blti95 blti50 blti46 blti80 blti3 blti4 blti59 blti3 blti9 blti06 blge07 blti98 blti08 blti5 blti92 blti75 blge02 blge80 blti0 blti02 blti04 blti76 blti62 blti44 blti72 blge08 blti87 blti24 blti05 blti3 blti6 blge27 blti56 blti09 blti69 blge0 blti7 blti48 blti35 blti32 blti99 blti90 Ogu03 blge63 blge64 blge52 blti09 blge0 blti49 blti79 blge57 blge35 blge7 blge3g blti0 blge20 blge03 blti2 blge05 blge56 blge42 blge29 blge3 blge04 blge33 blge66 blge5 blge50 blge53 blge6 blge67 blge68 blge62 blti65 blge73 blge37 blge43 blge44 blge40 blge70 blge4 blge89 blge2 blge39 blco07 blco06 blco30 blco3 blti85 blti24 blti28 *
53 98 blti30 SP blge06 RS blti33 RS blti60 SP blti34 RS blti0 RS blge46 RS blge 79 RS blti7 SP blge RS Lco02 blti47 RS blti68 RS blti30 PAR blti29 PAR blti74 SP blti89 PR blti RS blti93 PR blti95 RS blti50 RS blti46 RS blti80 RS blti3 RS blti4 RS blti59 SP blti3 RS blti9 RS 62 blti06 RS blge07 RS blti98 RS blti08 RS blti5 RS blti92 PR blti75 SP blge02 RS blge80 RS blti0 RS blti02 RS blti04 RS blti76 SP blti62 SP blti44 SC blti72 MS blge08 RS blti87 SP blti24 RS blti05 RS blti3 PR blti6 SP blge27 RS blti56 SP blti09 MG blti69 RS blge0 RS blti7 RS blti48 RS blti35 RS blti32 RS blti99 RS blti90 PR blge63 BOL blge64 BOL blge52 ARG blti09 RS blge0 RS blti49 RS blti79 RS blge57 URU blge35 RS blge7 RS blge3 RS blti0 RS blge20 URU blge03 RS blti2 RS blge05 RS blge56 URU blge42 RS blge29 RS blge3 RS blge04 RS blge33 RS blge66 BOL blge5 ARG blge50 ARG blge53 ARG blge6 ARG blge67 BOL blge68 BOL blge62 BOL blti65 SP blge73 RS blge37 RS blge43 RS blge44 RS blge40 RS blge70 RS blge4 RS blge89 RS blge2 RS blge39 RS Ogu03 70 blco07 blco30 blti85 blti24 blti28 blco06 blco3 Brazilian populations of L. tigrinus may have mtdna lineages from three distinct species!
54 Haplotype network (mtdna) Leopardus tigrinus Clade Leopardus geoffroyi Clade 48 (+ 3) Trigo et al. (2008) Mol. Ecol
55 Hypothesis - recent demographic expansion of L. tigrinus - secondary contact with closely related species Subsequent steps: Increased use of multilocus data sets - Morpho-ecological analyses
56 Expanded sampling 0 o MA CE PI PE SE N 30 ºS BOL ARG PAR MS PR GO SP SC MG RJ ES Central Region Northeastern Region A CH URU RS Southern Region Leopardus tigrinus Leopardus geoffroyi Leopardus colocolo 60 ºW
57 mtdna haplotype networks L. geoffroyi clade L. tigrinus clade L. guigna L. pardalis L. wiedii L. colocolo clade L. tigrinus (C, NE Brasil)
58 Haplotype network (intron 2, PLP gene, X chromosome) L. geoffroyi L. guigna L. colocolo L. pardalis + L. wiedii
59 Haplotype network (intron 2, PLP gene, X chromosome) L. tigrinus (S, SE Brasil) L. tigrinus (C, NE Brasil) L. guigna L. pardalis + L. wiedii
60 Haplotype network (intron 2, ZFY and SMCY3 genes, Y chromosome) L. tigrinus (S, SE Brasil) L. geoffroyi L. tigrinus (C, NE Brasil) L. colocolo L. wiedii
61 Bayesian analysis of microsatellites L. geoffroyi L. tigrinus (S, SE Brasil) L. tigrinus (C, NE Brasil) L. colocolo
62 q Detailed analyses of the hybrid zone: individuals rank
63 Score (76.9%) Canonic analysis based on 24 body measurements GM GF TM TF Score 2 (7.7%)
64 Correspondence analysis between genetic and morphological categories GPG LGE LTI TPT HIB GPI TPI
65 Individual case studies: - Morphological identification - mtdna haplotype - Y chromosome haplotype - PLP (X chrom.) haplotypes - microsatellite-based assignment (quantification of genome composition)
66 blge46 Canela, RS? Microsatellites 3rd Generation PLP: L. geoffroyi Zfy L. tigrinus mtdna: L. tigrinus
67 blti79 Guaíba, RS Microsatellites st Generation PLP: L. tigrinus + L. geoffroyi mtdna: L. geoffroyi
DNA barcoding of South American mammals
The Biota-FAPESP International Symposium on DNA Barcoding 29 DNA barcoding of South American mammals Eduardo Eizirik Lab. Biologia Genômica e Molecular, PUCRS, Brasil Instituto Pró-Carnívoros, Brasil Laboratory
The Brazilian Barcode of Life (BrBOL) initiative and its potential to aid in biodiversity conservation
DNA Barcodes 2015 Guelph, Canada, August 21st, 2015 The Brazilian Barcode of Life (BrBOL) initiative and its potential to aid in biodiversity conservation Eduardo Eizirik Faculdade de Biociências, PUCRS,
Optimizing Phylogenetic Supertrees Using Answer Set Programming
1 Online appendix for the paper Optimizing Phylogenetic Supertrees Using Answer Set Programming published in Theory and Practice of Logic Programming LAURA KOPONEN and EMILIA OIKARINEN and TOMI JANHUNEN
Supporting Information
Supporting Information Table S1. Sources of the historic range maps used in our analysis. Elevation limits (lower and upper) are in meters. Modifications to the source maps are listed in the footnotes.
Introduction Histories and Population Genetics of the Nile Monitor (Varanus niloticus) and Argentine Black-and-White Tegu (Salvator merianae) in
Introduction Histories and Population Genetics of the Nile Monitor (Varanus niloticus) and Argentine Black-and-White Tegu (Salvator merianae) in Florida JARED WOOD, STEPHANIE DOWELL, TODD CAMPBELL, ROBERT
Supplementary Materials for
www.sciencemag.org/content/343/6167/1241484/suppl/dc1 Supplementary Materials for Status and Ecological Effects of the World s Largest Carnivores William J. Ripple,* James A. Estes, Robert L. Beschta,
Cite Reference: Mellen, J.D. (1997) Minimum Husbandry Guidelines for Mammals: Small Felids. American Association of Zoos and Aquariums
ZOO STANDARDS FOR KEEPING SMALL FELIDS IN CAPTIVITY Jill D. Mellen, Disney's Animal Kingdom, PO Box 10000, Lake Buena Vista, FL 342830 Cite Reference: Mellen, J.D. (1997) Minimum Husbandry Guidelines for
CATS. Evolution. The. Elegant and enigmatic, cats tantalize not only those of us. By Stephen J. O Brien and Warren E. Johnson
GENETICS The Evolution of CATS Genomic paw prints in the DNA of the world s wild cats have clarified the cat family tree and uncovered several remarkable migrations in their past By Stephen J. O Brien
Original Article The comparison of the Felidae species with karyotype symmetry/asymmetry index (S/A I )
Punjab Univ. J. Zool., Vol. 32 (2), pp. 229-235, 2017 ISSN 1016-1597(Print) ISSN2313-8556 (online) Original Article The comparison of the Felidae species with karyotype symmetry/asymmetry index (S/A I
Molecular Genetics and Evolution of Melanism in the Cat Family
Current Biology, Vol. 13, 448 453, March 4, 2003, 2003 Elsevier Science Ltd. All rights reserved. PII S0960-9822(03)00128-3 Molecular Genetics and Evolution of Melanism in the Cat Family 3 Nestlé Purina
PROGRESS REPORT Report date Principle Researcher Affiliated organization Project Title Project theme Title
PROGRESS REPORT Report date: January 2019 Principle Researcher: Prajwol Manandhar Affiliated organization: Center for Molecular Dynamics Nepal (CMDN) Project Title: Developing cost-effective molecular
SCIENCE CHINA Life Sciences. Mitogenomic analysis of the genus Panthera
SCIENCE CHINA Life Sciences RESEARCH PAPERS October 2011 Vol.54 No.10: 917 930 doi: 10.1007/s11427-011-4219-1 Mitogenomic analysis of the genus Panthera WEI Lei 1,2, WU XiaoBing 1*, ZHU LiXin 3 & JIANG
110th CONGRESS 1st Session H. R. 1464
HR 1464 IH 110th CONGRESS 1st Session H. R. 1464 To assist in the conservation of rare felids and rare canids by supporting and providing financial resources for the conservation programs of nations within
The melanocortin 1 receptor (mc1r) is a gene that has been implicated in the wide
Introduction The melanocortin 1 receptor (mc1r) is a gene that has been implicated in the wide variety of colors that exist in nature. It is responsible for hair and skin color in humans and the various
A Genetic Comparison of Standard and Miniature Poodles based on autosomal markers and DLA class II haplotypes.
A Genetic Comparison of Standard and Miniature Poodles based on autosomal markers and DLA class II haplotypes. Niels C. Pedersen, 1 Lorna J. Kennedy 2 1 Center for Companion Animal Health, School of Veterinary
Reptilia, Squamata, Amphisbaenidae, Anops bilabialatus : Distribution extension, meristic data, and conservation.
Reptilia, Squamata, Amphisbaenidae, Anops bilabialatus : Distribution extension, meristic data, and conservation. Tamí Mott 1 Drausio Honorio Morais 2 Ricardo Alexandre Kawashita-Ribeiro 3 1 Departamento
CUMULATIVE ENVIRONMENTAL IMPACTS AND EXTINCTION RISK OF BRAZILIAN CARNIVORES
Oecologia Australis 21(3): 350-360, 2017 10.4257/oeco.2017.2103.11 CUMULATIVE ENVIRONMENTAL IMPACTS AND EXTINCTION RISK OF BRAZILIAN CARNIVORES Beatriz de Mello Beisiegel Floresta Nacional de Capão Bonito.
Species: Panthera pardus Genus: Panthera Family: Felidae Order: Carnivora Class: Mammalia Phylum: Chordata
CHAPTER 6: PHYLOGENY AND THE TREE OF LIFE AP Biology 3 PHYLOGENY AND SYSTEMATICS Phylogeny - evolutionary history of a species or group of related species Systematics - analytical approach to understanding
GEODIS 2.0 DOCUMENTATION
GEODIS.0 DOCUMENTATION 1999-000 David Posada and Alan Templeton Contact: David Posada, Department of Zoology, 574 WIDB, Provo, UT 8460-555, USA Fax: (801) 78 74 e-mail: dp47@email.byu.edu 1. INTRODUCTION
The cat family is placed within the order Carnivora,
The family of cats delineation of the feline basic type 1 Barnabas Pendragon and Niko Winkler Based on previously reported hybridizations, cats have long been considered to belong to a single basic type.
Bayesian Analysis of Population Mixture and Admixture
Bayesian Analysis of Population Mixture and Admixture Eric C. Anderson Interdisciplinary Program in Quantitative Ecology and Resource Management University of Washington, Seattle, WA, USA Jonathan K. Pritchard
Clarifications to the genetic differentiation of German Shepherds
Clarifications to the genetic differentiation of German Shepherds Our short research report on the genetic differentiation of different breeding lines in German Shepherds has stimulated a lot interest
PROGRESS REPORT for COOPERATIVE BOBCAT RESEARCH PROJECT. Period Covered: 1 October 31 December Prepared by
PROGRESS REPORT for COOPERATIVE BOBCAT RESEARCH PROJECT Period Covered: 1 October 31 December 2013 Prepared by John A. Litvaitis, Tyler Mahard, Marian K. Litvaitis, and Rory Carroll Department of Natural
Ibridazione naturale e antropogenica
Ibridazione naturale e antropogenica Ettore Randi Laboratorio di Genetica ISPRA, sede di Ozzano Emilia (BO) ettore.randi@isprambiente.it Foto Davide Palumbo Foto Giancarlo Tedaldi Images dowloaded for
The Rufford Foundation Final Report
The Rufford Foundation Final Report Congratulations on the completion of your project that was supported by The Rufford Foundation. We ask all grant recipients to complete a Final Report Form that helps
The Cryptic African Wolf: Canis aureus lupaster Is Not a Golden Jackal and Is Not Endemic to Egypt
: Canis aureus lupaster Is Not a Golden Jackal and Is Not Endemic to Egypt Eli Knispel Rueness 1, Maria Gulbrandsen Asmyhr 1, Claudio Sillero-Zubiri 2, David W. Macdonald 2, Afework Bekele 3, Anagaw Atickem
Seroprevalence and Genomic Divergence of Circulating Strains of Feline Immunodeficiency Virus among Felidae and Hyaenidae Species
JOURNAL OF VIROLOGY, July 2005, p. 8282 8294 Vol. 79, No. 13 0022-538X/05/$08.00 0 doi:10.1128/jvi.79.13.8282 8294.2005 Copyright 2005, American Society for Microbiology. All Rights Reserved. Seroprevalence
Lesson Overview. Human Chromosomes. Lesson Overview Human Chromosomes
Lesson Overview 14.1 Genome a full set of all the genetic information that an organism carries in its DNA. Karyotypes Karyotype a picture that shows the complete diploid set of human chromosomes, They
PARTIAL REPORT. Juvenile hybrid turtles along the Brazilian coast RIO GRANDE FEDERAL UNIVERSITY
RIO GRANDE FEDERAL UNIVERSITY OCEANOGRAPHY INSTITUTE MARINE MOLECULAR ECOLOGY LABORATORY PARTIAL REPORT Juvenile hybrid turtles along the Brazilian coast PROJECT LEADER: MAIRA PROIETTI PROFESSOR, OCEANOGRAPHY
Pavel Vejl Daniela Čílová Jakub Vašek Naděžda Šebková Petr Sedlák Martina Melounová
Czech University of Life Sciences Prague Faculty of Agrobiology, Food and Natural Resources Department of Genetics and Breeding Department of Husbandry and Ethology of Animals Pavel Vejl Daniela Čílová
Final Report for Research Work Order 167 entitled:
Final Report for Research Work Order 167 entitled: Population Genetic Structure of Marine Turtles, Eretmochelys imbricata and Caretta caretta, in the Southeastern United States and adjacent Caribbean region
Coyotes in Wolves' Clothing
Coyotes in Wolves' Clothing Author(s) :Tyler Wheeldon, Brent Patterson, and Dean Beyer Source: The American Midland Naturalist, 167(2):416-420. 2012. Published By: University of Notre Dame DOI: http://dx.doi.org/10.1674/0003-0031-167.2.416
Prof. Neil. J.L. Heideman
Prof. Neil. J.L. Heideman Position Office Mailing address E-mail : Vice-dean (Professor of Zoology) : No. 10, Biology Building : P.O. Box 339 (Internal Box 44), Bloemfontein 9300, South Africa : heidemannj.sci@mail.uovs.ac.za
+ Karyotypes. Does it look like this in the cell?
+ Human Heredity + Karyotypes A genome is the full set of genetic information that an organism carries in its DNA. Karyotype: Shows the complete diploid set of chromosomes grouped together in pairs, arranged
Assessment of coyote wolf dog admixture using ancestry-informative diagnostic SNPs
Molecular Ecology (2013) doi: 10.1111/mec.12570 Assessment of coyote wolf dog admixture using ancestry-informative diagnostic SNPs J. MONZ ON,* R. KAYS and D. E. DYKHUIZEN *Department of Molecular Genetics
INTRASPECIFIC AGONISM BETWEEN GIANT OTTER GROUPS. Carolina Ribas 1. Guilherme Mourão 2. Campo Grande, MS , Brazil. Brazil.
INTRASPECIFIC AGONISM BETWEEN GIANT OTTER GROUPS Carolina Ribas 1 Guilherme Mourão 2 1 Dept. de Biologia- CCBS, Universidade Federal de Mato Grosso do Sul, CP 549, Campo Grande, MS 79070-900, Brazil. 2
The Taming of the Cat Stephen J. O Brien. August 7, 2017 Some Pictures and Some Stories
The Taming of the Cat Stephen J. O Brien August 7, 2017 Some Pictures and Some Stories Beauceron Berger Allemand Borders Collies Westie Yorkshire Bouviers Bernois D.Bordeaux Whippet Epagneul Breton
Lab 8 Order Carnivora: Families Canidae, Felidae, and Ursidae Need to know Terms: carnassials, digitigrade, reproductive suppression, Jacobson s organ
Lab 8 Order Carnivora: Families Canidae, Felidae, and Ursidae Need to know Terms: carnassials, digitigrade, reproductive suppression, Jacobson s organ Family Canidae Canis latrans ID based on skull, photos,
INFORMATION TO USERS
INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter
Optimizing Phylogenetic Supertrees Using Answer Set Programming
Optimizing Phylogenetic Supertrees Using Answer Set Programming Laura Koponen 1, Emilia Oikarinen 1, Tomi Janhunen 1, and Laura Säilä 2 1 HIIT / Dept. Computer Science, Aalto University 2 Dept. Geosciences
Colonisation, diversificationand extinctionof birds in Macaronesia
Colonisation, diversificationand extinctionof birds in Macaronesia Juan Carlos Illera Research Unit of Biodiversity (UO-PA-CSIC) http://www.juancarlosillera.es / http://www.unioviedo.es/umib/ MACARONESIA
Beaver Canadian/North American Castor canadensis Chinchilla Chinchilla chinchilla/chinchilla lanigera/chinchilla lanigera forma domestica 1
ENGLISH LATIN Badger Taxidea taxus Bobcat (see Lynx cat) Felis rufa/lynx rufus/felis lynx rufus Beaver Canadian/North American Castor canadensis Chinchilla Chinchilla chinchilla/chinchilla lanigera/chinchilla
Temporal mitochondrial DNA variation in honeybee populations from Tenerife (Canary Islands, Spain)
Temporal mitochondrial DNA variation in honeybee populations from Tenerife (Canary Islands, Spain) Mª Jesús Madrid-Jiménez, Irene Muñoz, Pilar De la Rúa Dpto. de Zoología y Antropología Física, Facultad
Vet Integr Sci Veterinary Integrative Sciences. Genetic diversity and inbreeding situation of Korat and Siamese cats based on microsatellite markers
Research article Veterinary Integrative Science 2018; 16(3): XX-XX. Vet Integr Sci ISSN; 2629-9968 (online) Website; www.vet.cmu.ac.th/cmvj Genetic diversity and inbreeding situation of Korat and Siamese
2013 Holiday Lectures on Science Medicine in the Genomic Era
INTRODUCTION Figure 1. Tasha. Scientists sequenced the first canine genome using DNA from a boxer named Tasha. Meet Tasha, a boxer dog (Figure 1). In 2005, scientists obtained the first complete dog genome
Your web browser (Safari 7) is out of date. For more security, comfort and the best experience on this site: Update your browser Ignore
Your web browser (Safari 7) is out of date. For more security, comfort and the best experience on this site: Update your browser Ignore Activitydevelop BIG CATS AND THEIR HABITATS What do the different
THE CHEETAH (Acinonyx jubatus) IN NORTHERN AFRICA A NON-INVASIVE GENETIC STUDY OF CARNIVORES FROM THE AHAGGAR MOUNTAINS, SOUTHERN ALGERIA
THE CHEETAH (Acinonyx jubatus) IN NORTHERN AFRICA A NON-INVASIVE GENETIC STUDY OF CARNIVORES FROM THE AHAGGAR MOUNTAINS, SOUTHERN ALGERIA by GEORGE BUSBY BSc (Hons) A THESIS SUBMITTED IN PARTIAL FULFILMENT
Is there gene*c resistance of Nellore ca2le to both *cks and gastro-intes*nal parasites?
Is there gene*c resistance of Nellore ca2le to both *cks and gastro-intes*nal parasites? Fabio Luiz Buranelo Toral Department of Animal Science / UFMG Andrea Wilson s group GeneAcs and Genomics Division
WILDLIFE MONITORING AT MAYFLOWER BOCAWINA NATIONAL PARK DR BART HARMSEN, DR REBECCA FOSTER, REBECCA WOOLDRIDGE
WILDLIFE MONITORING AT MAYFLOWER BOCAWINA NATIONAL PARK DR BART HARMSEN, DR REBECCA FOSTER, REBECCA WOOLDRIDGE RANGE OF THE JAGUAR Jaguar populations are in decline due to: Habitat destruction Illegal
DNA BARCODING & MULTI-ISOTOPIC FINGERPRINTING: A NOVEL FORENSIC TOOLBOX FOR THE RAPID IDENTIFICATION OF ILLEGAL TRADE IN ENDANGERED WILDLIFE SPECIES
DNA BARCODING & MULTI-ISOTOPIC FINGERPRINTING: A NOVEL FORENSIC TOOLBOX FOR THE RAPID IDENTIFICATION OF ILLEGAL TRADE IN ENDANGERED WILDLIFE SPECIES Dissertation zur Erlangung des Doktorgrades (Dr. rer.
Conservation Genetics. Taylor Edwards University of Arizona Human Origins Genotyping Laboratory
Conservation Genetics Taylor Edwards University of Arizona Human Origins Genotyping Laboratory Conservation Biology The goal is to provide principles and tools for preserving biodiversity Conservation
DNA Offers New Insight Concerning Cat Evolution
January 6, 2006 Offers New Insight Concerning Cat Evolution By NICHOLAS WADE Researchers have gained a major insight into the evolution of cats by showing how they migrated to new continents and developed
Introduction to the Cheetah
Lesson Plan 1 Introduction to the Cheetah CRITICAL OUTCOMES CO #1: Identify and solve problems and make decisions using critical and creative thinking. CO #2: Work effectively with others as members of
Trends in Fisher Predation in California A focus on the SNAMP fisher project
Trends in Fisher Predation in California A focus on the SNAMP fisher project Greta M. Wengert Integral Ecology Research Center UC Davis, Veterinary Genetics Laboratory gmwengert@ucdavis.edu Project Collaborators:
A comparative acoustic analysis of purring in four cheetahs
A comparative acoustic analysis of purring in four cheetahs Robert Eklund 1, Gustav Peters 2, Florian Weise 3 and Stuart Munro 3 1 Department of Culture and Communication, Linköping University, Linköping,
Cystic echinococcosis in a domestic cat: an Italian case report
13th NRL Workshop, Rome, 24-25 May, 2018 Cystic echinococcosis in a domestic cat: an Italian case report Istituto Zooprofilattico Sperimentale (IZS) of Sardinia National Reference Laboratory for Cistic
AZA Felid Taxon Advisory Group North American Regional Collection Plan (RCP) 2 nd Edition.
AZA Felid Taxon Advisory Group North American Regional Collection Plan (RCP) 2 nd Edition 2009 www.felidtag.org Editor Norah Fletchall John Ball Zoo norah.fletchall@kentcountymi.gov 2 Felid Taxon Advisory
Evaluation of Noninvasive Genetic Sampling Methods for Felid and Canid Populations
Techniques and Technology Note Evaluation of Noninvasive Genetic Sampling Methods for Felid and Canid Populations EMILY W. RUELL, 1 Colorado State University, Department of Fish, Wildlife, and Conservation
The first felidlike carnivores appeared in. The Late Miocene Radiation of Modern Felidae: A Genetic Assessment REPORTS
16. O. Legasa, A. D. Buscalioni, Z. Gasparini, Stud. Geol. Salm. 29, 127 (1994). 17. Materials and methods are available as supporting material on Science Online. 18. The phylogenetic data set included
Chapter 10 ANIMALS. ARTICLE I Dogs License required; fees Rabies vaccination required. GENERAL REFERENCES
Chapter 10 ANIMALS [HISTORY: Adopted by the Town Board of the Town of Scott at time of adoption of Code (see Ch. 1, General Provisions, Art. II). Amendments noted where applicable.] Animal waste See Ch.
Biodiversity and Distributions. Lecture 2: Biodiversity. The process of natural selection
Lecture 2: Biodiversity What is biological diversity? Natural selection Adaptive radiations and convergent evolution Biogeography Biodiversity and Distributions Types of biological diversity: Genetic diversity
Evolution in dogs. Megan Elmore CS374 11/16/2010. (thanks to Dan Newburger for many slides' content)
Evolution in dogs Megan Elmore CS374 11/16/2010 (thanks to Dan Newburger for many slides' content) Papers for today Vonholdt BM et al (2010). Genome-wide SNP and haplotype analyses reveal a rich history
A southern California freeway is a physical and social barrier to gene flow in carnivores
Molecular Ecology (2006) 15, 1733 1741 doi: 10.1111/j.1365-294X.2006.02907.x Blackwell Publishing Ltd FAST-TRACK A southern California freeway is a physical and social barrier to gene flow in carnivores
ANNUAL PREDATION MANAGEMENT PROJECT REPORTING FORM
Nevada Department of Wildlife - Game Division ANNUAL PREDATION MANAGEMENT PROJECT REPORTING FORM Reporting Period: Due Date: 8/1/2015 Current Date: ######## 1) Project Name 2) Project Number 35 5) Project
Cytogenetic Study of the Leopard, Panthera pardus (Carnivora, Felidae) by Conventional Staining, G- banding and High-resolution Staining Technique
2008 The Japan Mendel Society Cytologia 73(1): 81 90, 2008 Cytogenetic Study of the Leopard, Panthera pardus (Carnivora, Felidae) by Conventional Staining, G- banding and High-resolution Staining Technique
Investigating the use of MPS for non-human and other specialist forensic applications
Investigating the use of MPS for non-human and other specialist forensic applications Jon Wetton DNA Evidence to Investigative Insights: Illumina - Berlin 19 th May 2016 MPS approaches to both human and
Phylogenetics, genome diversity and origin of modern
Molecular Ecology (2001) 10, 2617 2633 Phylogenetics, genome diversity and origin of modern Blackwell Science, Ltd leopard, Panthera pardus OLGA UPHYRKINA,* WARREN E. JOHNSON,* HOWARD QUIGLEY, DALE MIQUELLE,
UNIT 6 Genes and Inheritance sciencepeek.com
Part 1 - Inheritance of Genes Name Date Period 1. Fill in the charts below on the inheritance of genes. 2. In a diploid cell, there are copies of each chromosome present. 3. Each human diploid cell has
Systematics and taxonomy of the genus Culicoides what is coming next?
Systematics and taxonomy of the genus Culicoides what is coming next? Claire Garros 1, Bruno Mathieu 2, Thomas Balenghien 1, Jean-Claude Delécolle 2 1 CIRAD, Montpellier, France 2 IPPTS, Strasbourg, France
Spot the Difference: Using the domestic cat as a model for the nutritional management of captive cheetahs. Katherine M. Bell
Spot the Difference: Using the domestic cat as a model for the nutritional management of captive cheetahs Katherine M. Bell Edited by Lucy A. Tucker and David G. Thomas Illustrated by Justine Woosnam and
Mastozoología Neotropical ISSN: Sociedad Argentina para el Estudio de los Mamíferos. Argentina
Mastozoología Neotropical ISSN: 0327-9383 ulyses@cenpat.edu.ar Sociedad Argentina para el Estudio de los Mamíferos Argentina Lucherini, Mauro; Soler, Lucía; Luengos Vidal, Estela A preliminary revision
WILDCAT HYBRID SCORING FOR CONSERVATION BREEDING UNDER THE SCOTTISH WILDCAT CONSERVATION ACTION PLAN. Dr Helen Senn, Dr Rob Ogden
WILDCAT HYBRID SCORING FOR CONSERVATION BREEDING UNDER THE SCOTTISH WILDCAT CONSERVATION ACTION PLAN Dr Helen Senn, Dr Rob Ogden Wildcat Hybrid Scoring For Conservation Breeding under the Scottish Wildcat
6. The lifetime Darwinian fitness of one organism is greater than that of another organism if: A. it lives longer than the other B. it is able to outc
1. The money in the kingdom of Florin consists of bills with the value written on the front, and pictures of members of the royal family on the back. To test the hypothesis that all of the Florinese $5
Question 3 (30 points)
Question 3 (30 points) You hope to use your hard-won 7.014 knowledge to make some extra cash over the summer, so you adopt two Chinchillas to start a Chinchilla breeding business. Your Chinchillas are
Economically important trait. Increased demand: Decreased supply. Sheep milk cheese. 2007: $2.9 million for milk production (Shiflett, 2008)
Genetic Markers for Milk Production Raluca Mateescu, OklahomaStateUniversity Michael Thonney, Cornell University Milk production & Sheep Industry Economically important trait 2007: $2.9 million for milk
Lecture 11 Wednesday, September 19, 2012
Lecture 11 Wednesday, September 19, 2012 Phylogenetic tree (phylogeny) Darwin and classification: In the Origin, Darwin said that descent from a common ancestral species could explain why the Linnaean
Genetics for breeders. The genetics of polygenes: selection and inbreeding
Genetics for breeders The genetics of polygenes: selection and inbreeding Selection Based on assessment of individual merit (appearance) Many traits to control at the same time Some may be difficult to
Bi156 Lecture 1/13/12. Dog Genetics
Bi156 Lecture 1/13/12 Dog Genetics The radiation of the family Canidae occurred about 100 million years ago. Dogs are most closely related to wolves, from which they diverged through domestication about
Management. of genetic variation in local breeds. Asko Mäki-Tanila. Reykjavik 30/4/2009. Embryocentre Ltd
Management Embryocentre Ltd of genetic variation in local breeds Asko Mäki-Tanila Reykjavik 30/4/2009 based on collaboration with T Meuwissen, J Fernandez and M Toro within EURECA project Approach in two
Geoffroy s Cat: Biodiversity Research Project
Geoffroy s Cat: Biodiversity Research Project Viet Nguyen Conservation Biology BES 485 Geoffroy s Cat Geoffroy s Cat (Leopardus geoffroyi) are small, little known spotted wild cat found native to the central
Evaluating the net effects of climate change on tick-borne disease in Panama. Erin Welsh November 18, 2015
Evaluating the net effects of climate change on tick-borne disease in Panama Erin Welsh November 18, 2015 Climate Change & Vector-Borne Disease Wide-scale shifts in climate will affect vectors and the
Global diversity of cystic echinococcosis. Thomas Romig Universität Hohenheim Stuttgart, Germany
Global diversity of cystic echinococcosis Thomas Romig Universität Hohenheim Stuttgart, Germany Echinococcus: generalized lifecycle Cystic echinococcosis: geographical spread Acephalocystis cystifera
First named as a separate species of rodent in 1946, Tokudaia muenninki, also known as
First named as a separate species of rodent in 1946, Tokudaia muenninki, also known as Muennink s spiny rat or the Okinawa spiny rat, lives in the northern region of Yanbaru Forest on Okinawa Island, Japan.
Detection of hantavirus in bats from remaining rain forest in São Paulo, Brazil
Detection of hantavirus in bats from remaining rain forest in São Paulo, Brazil de Araujo et al. de Araujo et al. BMC Research Notes 2012, 5:690 de Araujo et al. BMC Research Notes 2012, 5:690 SHORT REPORT
Applying molecular genetic tools to tiger conservation
Integrative Zoology 2010; 5: 351-362 doi: 10.1111/j.1749-4877.2010.00222.x REVIEW Applying molecular genetic tools to tiger conservation Shu-Jin LUO, 1 Warren E. JOHNSON, 2 and Stephen J. O BRIEN 2 1 School
UNIT III A. Descent with Modification(Ch19) B. Phylogeny (Ch20) C. Evolution of Populations (Ch21) D. Origin of Species or Speciation (Ch22)
UNIT III A. Descent with Modification(Ch9) B. Phylogeny (Ch2) C. Evolution of Populations (Ch2) D. Origin of Species or Speciation (Ch22) Classification in broad term simply means putting things in classes
Characterization of Microsatellite Markers for the Siamese Crocodile and Amplification in the Closely Related Genus Crocodylus
Kasetsart J. (Nat. Sci.) 42 : 682-692 (2008) Characterization of Microsatellite Markers for the Siamese Crocodile and Amplification in the Closely Related Genus Crocodylus Win Chaeychomsri 1, 6*, Sudawan
Minnesota_mammals_Info_10.doc 11/09/09 -- DRAFT Page 11 of 50
Minnesota_mammals_Info_10.doc 11/09/09 -- DRAFT Page 11 of 50 Order Chiroptera Bats are the only mammals with wings and the only mammals that fly. Bats fly slower than birds, and all Minnesota bats are
Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences
Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 93(5): 695-702, Sep./Oct. 1998 Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences KL Haag, AM Araújo,
Genome 371; A 03 Berg/Brewer Practice Exam I; Wednesday, Oct 15, PRACTICE EXAM GENOME 371 Autumn 2003
PRACTICE EXAM GENOME 371 Autumn 2003 These questions were part of the first exam from Autumn 2002. Take the exam in a quiet place and only when you are sure you will have time to complete the exam uninterrupted.
Soraya Wijntuin, CI-SURINAME
ESTIMATING ABUNDANCE AND HABITAT-BASED POPULATION FOR OCELOT (LEOPARDUS PARDALIS) AND MARGAY (LEOPARDUS WIEDII) IN CENTRAL SURINAME NATURE RESERVE USING CAMERA TRAPPING Soraya Wijntuin, CI-SURINAME Table
International Journal of Veterinary Science
Research Article P-ISSN: 2304-3075; E-ISSN: 2305-4360 International Journal of Veterinary Science Maternal Phylogeny of Anatolıan Cats www.ijvets.com; editor@ijvets.com Nüket Bilgen*, Mustafa Yenal Akkurt,
Molecular diagnosis of Theileria infections in wildlife from Southern Africa ~ implications for accurate diagnosis.
Molecular diagnosis of Theileria infections in wildlife from Southern Africa ~ implications for accurate diagnosis. Ronel Pienaar Parasites Vectors and Vector-borne Diseases Onderstepoort Veterinary Institute
Dramatis personae: an introduction to the wild felids
CHAPTER 1 Dramatis personae: an introduction to the wild felids David W. Macdonald, Andrew J. Loveridge, and Kristin Nowell Pallas s cat # John Tobias What is a felid? For those in tune with the intricate
Density and activity pattern of Leopardus wiedii and Leopardus pardalis at Sierra Norte of Oaxaca, Mexico
THERYA, 2017, Vol. 8 (3): 217-222 DOI: 10.12933/therya-17-487 ISSN 2007-3364 Density and activity pattern of Leopardus wiedii and Leopardus pardalis at Sierra Norte of Oaxaca, Mexico Gabriela Pérez-Irineo
CRANIAL EVIDENCE FOR SEXUAL DIMORPHISM AND GROUP LIVING IN THE EXTINCT AMERICAN LION (PANTHERA LEO ATROX)
CRANIAL EVIDENCE FOR SEXUAL DIMORPHISM AND GROUP LIVING IN THE EXTINCT AMERICAN LION (PANTHERA LEO ATROX) Gold, David Department of Ecological and Evolutionary Biology Professor Matthew McHenry This study
Supplemental Information. A Deletion in the Canine POMC Gene. Is Associated with Weight and Appetite. in Obesity-Prone Labrador Retriever Dogs
Cell Metabolism, Volume 23 Supplemental Information A Deletion in the Canine POMC Gene Is Associated with Weight and Appetite in Obesity-Prone Labrador Retriever Dogs Eleanor Raffan, Rowena J. Dennis,
PROGRESS REPORT for COOPERATIVE BOBCAT RESEARCH PROJECT. Period Covered: 1 April 30 June Prepared by
PROGRESS REPORT for COOPERATIVE BOBCAT RESEARCH PROJECT Period Covered: 1 April 30 June 2014 Prepared by John A. Litvaitis, Tyler Mahard, Rory Carroll, and Marian K. Litvaitis Department of Natural Resources
A. Pulse-field gel of hummingbird genomic DNA. B. Bioanalyzer plot of hummingbird SMRTbell library
A. Pulse-field gel of hummingbird genomic DNA 1: Sheared gdna: 35 kb & 40 kb 2: BluePippin sizeselected library (17 kb cut-off) 3: Original gdna B. Bioanalyzer plot of hummingbird SMRTbell library 5kb
Searching for the endangered red-billed curassow in the Brazilian Atlantic Rainforest
Searching for the endangered red-billed curassow in the Brazilian Atlantic Rainforest Rufford Interim Report Red-billed curassows are endemic and threatened species of the Brazilian Atlantic Rainforest.