PRA-prcd DNA Test Case Number: Owner: Jessica Dowler PO Box 72 Britton SD Canine Information DNA ID Number: Call Name: Hooch Sex: F

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7 PRA-prcd DNA Test Case Number: Owner: Jessica Dowler PO Box 72 Britton SD Canine Information DNA ID Number: Call Name: Hooch Sex: Female Birthdate: 03/21/2014 Breed: Labrador Retriever Coat Color: Yellow Registered Name: FR's Homemade Hooch Registration Number: SR Microchip/Tattoo: Report Date: DNA Result: 1/5/2017 Clear (2 copies of the normal allele) These results are based on data obtained from analysis of unique DNA loci in accordance with the standards and protocols set forth by DDC Veterinary. The accuracy of the result is based on the information and the quality of samples provided by the client. DDC Veterinary does not assume responsibility of errors due to mislabeled or incorrectly sampled submissions. Matt Shaunessy, Senior Scientist

8 Canine Genetic Health Certificate Call Name: Hooch Registered Name: FR's Homemade Hooch Breed: Labrador Retriever Sex: Female DOB: March 2012 Laboratory #: Registration #: SR Microchip #: Certificate Date: March 19, 2018 This canine's DNA showed the following genotype(s): Disease Gene Genotype Interpretation Retinal Dysplasia/Oculoskeletal Dysplasia 1 COL9A3 WT/WT Normal (clear) WT, wild type (normal); M, mutant; Y, Y chromosome (male) Blake C Ballif, PhD Laboratory & Scientific Director Casey R Carl, DVM Associate Medical Director Paw Print Genetics performed the tests listed on this dog. See the Laboratory Report for interpretation and recommendations based on these findings. The genes/diseases reported here were selected by the client. Normal results do not exclude inherited mutations not tested in these or other genes that may cause medical problems or may be passed on to offspring. These tests were developed and their performance determined by Paw Print Genetics. This laboratory has established and verified the tests' accuracy and precision. Because all tests performed are DNA-based, rare genomic variations may interfere with the performance of some tests producing false results. If you think these results are in error, please contact the laboratory immediately for further evaluation. In the event of a valid dispute of results claim, Paw Print Genetics will do its best to resolve such a claim to the customer s satisfaction. If no resolution is possible after investigation by Paw Print Genetics with the cooperation of the customer, the extent of the customer's sole remedy is a refund of the fee paid. In no event shall Paw Print Genetics be liable for indirect, consequential or incidental damages of any kind. Any claim must be asserted within 60 days of the report of the test results. Genetic counseling is available at Paw Print Genetics. Page 1 of 1

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14 Degenerative Myelopathy DNA Test Case Number: Owner: Jessica Dowler PO Box 72 Britton SD Canine Information DNA ID Number: Call Name: Hooch Sex: Female Birthdate: 03/21/2012 Breed: Labrador Retriever Coat Color: Yellow Registered Name: FR's Homemade Hooch Registration Number: SR Microchip/Tattoo: Report Date: 10/15/2015 DNA Result: Clear (2 copies of the normal allele) Matt Shaunessy, Senior Scientist

15 Hyperuricosuria DNA Test Case Number: Owner: Jessica Dowler PO Box 72 Britton SD Canine Information DNA ID Number: Call Name: Hooch Sex: Female Birthdate: 03/21/2012 Breed: Labrador Retriever Coat Color: Yellow Registered Name: FR's Homemade Hooch Registration Number: SR Microchip/Tattoo: Report Date: 10/15/2015 DNA Result: Clear (2 copies of the normal allele) Matt Shaunessy, Senior Scientist

16 Cystinuria DNA Test Case Number: Owner: Jessica Dowler PO Box 72 Britton SD Canine Information DNA ID Number: Call Name: Hooch Sex: Female Birthdate: 03/21/2012 Breed: Labrador Retriever Coat Color: Yellow Registered Name: FR's Homemade Hooch Registration Number: SR Microchip/Tattoo: Report Date: 10/15/2015 DNA Result: Clear (2 copies of the normal allele) Matt Shaunessy, Senior Scientist

17 Coat Color DNA Test Case Number: Owner: Jessica Dowler PO Box 72 Britton SD Canine Information DNA ID Number: Call Name: Hooch Sex: Female Birthdate: 03/21/2012 Breed: Labrador Retriever Coat Color: Yellow Registered Name: FR's Homemade Hooch Registration Number: SR Microchip/Tattoo: Report Date: 10/15/2015 DNA Result: DD C.22G>A -/- Matt Shaunessy, Senior Scientist

18 This supplemental sheet can be used as a guide to help clients better understand their DNA Coat Color results. More comprehensive information about DNA Color testing can be found at our webpage: BB S41C -/-, Q331X -/-, 345delP -/- (does not carry brown) Bb S41C +/-, Q331X -/-, 345delP -/- (brown carrier) Bb S41C -/-, Q331X +/-, 345delP -/- (brown carrier) Bb S41C -/-, Q331X -/-, 345delP +/- brown carrier) Bb 2 S41C +/-, Q331X -/-, 345delP +/- (carries 2 copies of brown alleles) bb S41C, Q331X, 345delP (brown phenotype; 2 or more SNPs detected) *Please note that brown color is also commonly referred to as liver or chocolate and occasionally red in a few breeds as well. EE R306ter -/- (does not carry yellow) Ee R306ter +/- (yellow carrier) ee R306ter +/+ (yellow phenotype) *Please note that yellow color in Labrador Retrievers can be interpreted differently in other breeds. The phenotype could include a number of lighter colors described by breeders as cream, white, clear red, red, or apricot. DD C.22G>A -/- (does not carry dilution) Dd C.22G>A +/- (dilute carrier) dd C.22G>A +/+ (dilute phenotype) E M E M M264V +/+ (2 copies of dominant mask allele) E M E x M264V +/- (1 copy of dominant mask allele & 1 copy of recessive non-mask allele) E x E x M264V -/- (2 copies of recessive non-mask allele) NN spot SINE -/- (2 copies of the non-piebald allele) NS spot SINE +/- (1 copy of the non-piebald allele and 1 copy of the piebald allele) SS spot SINE +/+ (2 copies of the piebald allele) K B K B G23del +/+ (2 copies of dominant allele) K B K y G23del +/- (1 copy of dominant allele & 1 copy of recessive allele) K y K y G23del -/- (2 copies of recessive allele) a y a y A82S +/+ (2 copies of fawn/sable allele) a y a w A82S +/- (1 copy of fawn/sable allele & 1 copy of non-fawn/sable allele) a w a w A82S -/- (2 copies of non- fawn/sable allele) aa R96C +/+ (2 copies of recessive black allele) aa x a x a x R96C R96C +/- -/- (1 copy of recessive black allele & 1 copy of non-recessive black allele) (2 copies of non-recessive black allele) a w a w tan SINE -/- (2 copies of the non-tan point allele) a w a t tan SINE +/- (1 copy of the non-tan point allele and 1 copy of the tan point allele) a t a t tan SINE +/+ (2 copies of the tan point allele) NN PSMB7:c.146T>G -/- (does not carry harlequin) NH PSMB7:c.146T>G +/- (1 copy of the harlequin, harlequin is expressed if merle gene is also present)

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20 DOG GENETIC DIVERSITY ANALYSIS FR'S HOMEMADE HOOCH Case: NCD73775 Print Date: March 20, 2018 Report ID: Labrador Retriever IR Diversity Panel Frequency Breed: Labrador Retriever Sex: Female Color: EEBBDD DOB: 2012 Reg: SR Most Outbred Labrador Retriever DLA IR = 0.11 (0.07 to 0.15) Most Inbred Internal relatedness (IR) is an allele frequency weighted indicator of homozygosity. IR values of siblings vary due to segregation but will increase on average with inbreeding. Lower IR values indicate greater genetic diversity. IR is calculated relative to breed. Haplotype 1 Haplotype 2 Veterinary Genetics Laboratory One Shields Avenue, Davis, CA DLA I DLA II Maintaining diversity in the DLA which helps regulate immune responses is beneficial to a breed. Choosing mates differing in their DLA haplotypes helps maintain diversity in litters. JESSICA DOWLER ND AVE, PO BOX 72 BRITTON, SD 57430

21 CANINE GENETIC MARKER REPORT JESSICA DOWLER ND AVE, PO BOX 72 BRITTON, SD Case: Date Received: NCD Jan-2018 Print Date: 20-Mar-2018 Report ID: Verify report at Name: FR'S HOMEMADE HOOCH Reg: SR DOB: 03/21/2012 Sex: Female Breed: Labrador Retriever Microchip: Color: EEBBDD Sire: WATERMARK'S BAY CREEK RASCAL Reg: SN Dam: FR'S ELEGANT GRACE Reg: SR DIVERSITY PANEL LOCUS TYPE LOCUS TYPE LOCUS TYPE 1: AHT /108 2: AHT /141 3: AHTH /129 4: AHTh171-A 223/227 5: AHTh /244 6: AHTk211 87/95 7: AHTk /290 8: C /126 9: FH /148 10: FH /164 11: FH /240 12: INRA21 101/95 13: INU /126 14: INU /152 15: INU /212 16: LEI /95 17: REN105L03 231/235 18: REN162C04 202/210 19: REN169D01 202/202 20: REN169O18 170/170 21: REN247M23 268/268 22: REN54P11 222/232 23: REN64E19 147/147 24: VGL / : VGL / : VGL1063 8/13 27: VGL /28 28: VGL /22 29: VGL /13 30: VGL /17 31: VGL /14 32: VGL /21 33: VGL /16 The Veterinary Genetics Laboratory is an institutional member of ISAG. DNA types are reported according to standardized nomenclature for markers in the ISAG panel.