Defensive behavior in Leptodactylus vastus A. LuTZ, 1930, in northeastern Brazil

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1 214 SHORT NOTE HERPETOZOA 29 (3/4) Wien, 30. Jänner 2017 SHORT NOTE J. C. & GRiffiTHS, R. A. (2000): Amphibians and reptiles: A natural history of the British herpetofauna. Lon - don (HarperCollins), pp BiLLE, T. (2008): Dan - marks padder og krybdyr en feltguide fra danske-dyr. dk; Næstved (ConDidact), pp. 55. BuSCHENDORf, J. (2004): Ringelnatter Natrix natrix (LiNNAEuS, 1758); pp in: MEyER, f. & BuSCHENDORf, J. & ZuPPkE, u. & BRAuMANN, f. & SCHäDLER, M. & GROSSE, W.-R. (Eds.): Die Lurche und kriechtiere Sachsen-Anhalts Verbreitung, Ökologie, Gefährdung und Schutz.- Supplement der Zeitschrift für feldherpe - tologie 3. Bielefeld (Laurenti). DüRiGEN, B. (1897): Deutschlands Amphibien und Reptilien. Eine Beschrei - bung und Schilderung sämmtlicher in Deutschland und den angrenzenden Gebieten vorkommenden Lurche und kriechthiere. Magdeburg (Creutz sche Verlags - buchhandlung), pp ECkSTEiN, H.-P. (1993): un - tersuchungen zur Ökologie der Ringelnatter (Natrix natrix LiNNAEuS, 1758).- Jahrbuch für feldherpetolo - gie, Beiheft; 4: fellenberg, W. (1981): Ringel - natter Natrix natrix (LiNNAEuS, 1758); pp in: feldmann, R. (Ed.): Die Amphibien und Reptilien Westfalens.- Abhandlungen aus dem Landesmuseum für Naturkunde zu Münster in Westfalen, Münster; 43. kabisch, k. (1999): Natrix natrix (LiNNAEuS, 1758) Ringelnatter; pp in: BÖHME, W. (Ed.): Hand - buch der Reptilien und Amphibien Europas; Band 3/iiA: Schlangen ii; Serpentes ii: Colubridae 2 (Boigi - nae, Natricinae). Wiebelsheim (AuLA-Verlag). ROLLi - NAT, R. (1946): La vie des reptiles de la france Cent - rale; Paris (Librairie Delagrave), pp SCHLEiCH, H. H. & kästle, W. & kabisch, k. (1996): Amphi - bians and reptiles of North Africa; koenigstein (koeltz Scientific Books), pp. X, SMiTH, M. (1951): The British amphibians & reptiles; London (Collins), pp. xiv, 318. STEWARD, J. W. (1971): The snakes of Europe; Newton Abbot (David & Charles), pp STREET, D. (1979): The reptiles of northern and central Europe; Lon - don (B. T. Batsford), pp VAuGHAN, R. (2008): An incidence of Natrix natrix helvetica observed in arboreal mating.- Herpetological Bulletin, London; 103: VAuGHAN, R. (2012): Grass Snakes. Third Edition; Privately published, pp VEiTH, G. (1991): Die Reptilien Bosniens und der Herzegowina, Teil ii.- Herpetozoa, Wien; 4: WAiTZMANN, M. & SOWiG, P. (2007): Ringelnatter Natrix natrix (LiNNAEuS, 1758); pp in: LAufER, H. & fritz, k. & SOWiG, P. (Eds.): Die Amphibien und Reptilien Baden-Württem - bergs; Stuttgart (Eugen ulmer). WiLDSCREEN ARCHiVE (2016): Grass snake (Natrix natrix). WWW document (movie), available at < > (Last accessed: March 28, 2016). ZiMMERMANN, R. (1908): Der deut - schen Heimat kriechtiere und Lurche; Stuttgart (fritz Lehmann), pp. 191 pp. ZiMMERMANN, R. (1909): Zur Schlangenfauna von Rochlitz i. S.- Lacerta: Zeitschrift für Aquarien- und Terrarienkunde, Braunschweig; 21: 81-83, 22: key WORDS: Reptilia: Squamata: Serpentes: Colubridae; Natrix natrix, height-seeking, climbing ability, arboreality, behavior, Denmark SuBMiTTED: November 16, 2015 AuTHORS: Henrik BRiNGSøE (Corresponding author, < bringsoe@ .dk >) irisvej 8, Dk-4600 køge, Denmark & Peter AASTRuP Aarhus university, Department of Bioscience, frederiksborgvej 399, Dk Roskilde, Denmark. Defensive behavior in Leptodactylus vastus A. LuTZ, 1930, in northeastern Brazil The entire defensive repertoire of a species or a population typically evolves due to strong selective pressure exerted by their natural predators (GREENE 1997; VAMOSi 2005). The most common defensive strategies of mobile animals are either to remain motionless or to try to escape from potential predators. However, a variety of defensive strategies can be used de - pending on the risk posed by the predator (TOLEDO et al. 2011). Anurans exhibit a variety of defensive behaviors (DODD 1976; WiLLiAMS et al. 2000; TOLEDO et al. 2005, 2010, 2011), in - cluding combinations of ecological, morpho - logical, physiological, or behavioral characteristics (DuELLMAN & TRuEB 1994; TOLE - DO & JARED 1995), and this flexibility may improve their chances of survival (MARCHi - SiN & ANDERSON 1978). The skin of amphibians plays an im - portant role in defense against predators and microorganisms possessing dermal mucous and granular glands responsible for the secretion of mucus and toxins, respectively (STEBBiNS & COHEN 1995; TOLEDO & JARED 1995; BARBOSA et al. 2015). A great variety of chemical compounds can be found in the secretion of granular glands. in many spe - cies these glands form aggregates, or macroglands (TOLEDO & JARED 1995), which are strategically placed on the body surface. in frogs of the family Leptodactylidae, macroglands are present on the skin of the dorsum (TOLEDO & JARED 1995) and usually exhibit bright colors that are exposed during dei - matic behavior. This is a report on three observations of defensive behaviour exhibited by Lepto - dactylus vastus A. LuTZ, 1930, recorded in an area of the type Brejo de Altitude, in northeastern Brazil. Leptodactylus vastus is a leaf-litter frog of the L. pentadactylus group (LAuRENTi, 1768), and like other members of this group it is large, reaching about 20 cm snout-vent length (SVL) (HEyER 2005; DE Sá et al. 2014). This species is often found near water bodies in areas with typical Atlantic

2 SHORT NOTE HERPETOZOA 29 (3/4) Wien, 30. Jänner 2017 SHORT NOTE 215 fig. 1: Leptodactylus vastus A. LuTZ, 1930, performing thanatosis. Photo by D. P. Castro. forest and Caatinga phytophysiognomies (frost 2015). it is widely distributed in the Brazilian state of Ceará, occurring in areas of Caatinga (BORGES-NOJOSA et al. 2010; SANTANA et al. 2015), coastal regions (BOR- GES-LEiTE et al. 2014) and Brejos de Alti - tude (BORGES-NOJOSA 2007; LOEBMANN & HADDAD 2010; RiBEiRO et al. 2012). On April 14, 2015, working in a project on beta diversity of litter amphibians in ubajara National Park (PARNA-ubajara), Ceará ( S / W), the authors observed an individual of L. vastus performing three types of defensive be - havior when it was manipulated for taking photographs and body measurements in the laboratory. The first behavioral strategy was tha - natosis (death feigning) by exposing its ab - domen and retracting the front feet close to the body (fig. 1). This behavior lasted about seven minutes, after which the individual returned to a normal body position. After thanatosis, the specimen performed partial body-raising (TOLEDO et al. 2011), stretching its legs and lifting the body from the ground while keeping its snout in contact with the substrate. The individual remained motionless in this position for about five minutes, while the reddish color in its inguinal region was exposed (figs. 2 and 3). immediately after the specimen exhibited this posture, the manipulators began to feel their eyes and noses become irritated. Thanatosis is often displayed by amphibians (GALLy et al. 2014), and has been reported for multiple families, including Bufonidae (ZAMPROGNO et al. 1998), Cycloramphidae (HARTMANN et al. 2003), Hylidae (AZEVEDO-RAMOS 1995), Micro - hylidae (BORGES-LEiTE et al. 2012) and Odontophrynidae (BEZERRA et al. 2010; BORGES-NOJOSA et al. 2016). Remaining motionless can help avoid predators that are visually oriented or divert predator attention (MiyATAkE et al. 2009; TOLEDO et al. 2010). Records of thanatosis among leptodactylid frogs are common (TOLEDO et al. 2005) and have been recorded for Leptodactylus cha - quensis CEi, 1950 (LOuRENçO-DE-MORAES et al. 2014), Leptodactylus fuscus (SCHNEi- DER, 1799) (TOLEDO et al. 2010) and Lepto - dactylus mystacinus (BuRMEiSTER, 1861) (TOLEDO et al. 2010). Among the species of the L. pentadactylus group, only L. laby - rinthicus (SPiX, 1824) has been reported to perform thanatosis (TOLEDO et al. 2005). However, to our knowledge, there are no previous reports of thanatosis behavior for L. vastus in the literature. The behavior of body-raising is cited by TOLEDO et al. (2011), as one of 30 defensive behaviors performed by amphibians. This behavior is usually exhibited by toxic species, and can be displayed in two different modes: (1) partial, when the individual stretches the legs vertically and maintains the snout close to or touching the ground and (2) full, when the individual extends both anterior and posterior limbs and raises its belly and snout (TOLEDO et al. 2011). This behavior is reported for the families Aromobatidae (TOLEDO et al. 2011) and Bufonidae (ESCOBAR-LASSO & GONZáLEZ-

3 ALL_SHORT_NOTES:SHORT_NOTE.qxd :36 Seite SHORT NOTE HERPETOZOA 29 (3/4) Wien, 30. Jänner 2017 SHORT NOTE fig. 2: Leptodactylus vastus A. LuTZ, 1930, performing partial body-raising, keeping its snout touching the substrate while the hind legs remain extended. Photo by D. P. Castro. fig. 3: Behavior of body-raising in Leptodactylus vastus A. LuTZ, 1930, showing how the individual exposes the reddish regions of the inguinal region while releasing noxious substances that irritate the mucous membranes of potential predators. Photo by D. P. Castro.

4 SHORT NOTE HERPETOZOA 29 (3/4) Wien, 30. Jänner 2017 SHORT NOTE 217 DuRAN 2012), and is probably used to make subjugation more difficult when the individual is captured (TOLEDO et al. 2011) and seems to be closely associated with the presence of noxious or odoriferous skin secretions (HÖDL & AMéZquiTA 2001). The production of secretions which is also considered a method of defense for amphibians (TOLEDO et al. 2011) is de - scribed in Leptodactylidae (HADDAD et al. 2013), such as the species group of Lepto - dactylus latrans (STEffEN, 1815), the latter producing noxious secretions that leave the skin slippery (LOuRENçO-DE-MORAES et al. 2014). Members of the L. pentadactylus group, including L. vastus, also produce and secrete toxic substances from the skin (HADDAD et al. 2013). These substances can cause sensations of severe burning when in contact with the mouth, eyes or nostrils (TOLEDO et al. 2011; HADDAD et al. 2013), which is probably why frogs of this group are referred to as pepper-frogs. Combining different defensive strategies can increase the chances of escape from a predators attack (TOLEDO et al. 2011). Ac cording to TOLEDO et al. (2005), species within a group of closely related amphibian spe cies can exhibit similar defensive strategies, which is a result of phylogenetic relationships rather than ecological niches. How ever, lack of knowledge of anti-predator be havior in different families of amphibians limits meta-analyses regarding defensive stra tegies (LOuRENçO-DE-MORAES et al. 2014). ACkNOWLEDGMENTS: The authors thank G. J. B. MOuRA and E. 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(2005): On the role of enemies in divergence and diversification of prey: a review and synthesis.- Canadian Journal of Zoology, Ottawa; 83: WiLLiAMS, C. R. & BRODiE Jr. E. D. & TyLER, M. J. & WALkER, S. J. (2000): Anti pre - dator mechanisms of Australian frogs.- Journal of Her - petology, Houston; 34 (3): ZAMPROGNO, C. & ZAMPROGNO, M. G. f. & TEiXEiRA, R. L. (1998): Bufo paracnemis (Sapo-boi). Death feigning.- Herpetologi - cal Review; New york; 29 (2): key WORDS: Amphibia: Anura: Leptodactyli - dae; Leptodactylus vastus; ethology, antipredator be - havior, body-raising, thanatosis; Brazil SuBMiTTED: May 30, AuTHORS: Déborah P. DE CASTRO 1, 2) (Corre- sponding author, < deborahpraciano@gmail.com >), Diva M. BORGES-NOJOSA 1, 2), John A. A. OLiVEiRA 2), Maria J. BORGES-LEiTE 1, 2), Margarida M. X. DA SiLVA 2), Tiago A. DE SOuSA 1, 2) & David James HARRiS 3) 1) Programa de Pós-Graduação em Ecologia e Recursos Naturais, universidade federal do Ceará. Campus do Pici, Bloco 906, CEP , forta - leza - CE, Brazil; 2) Núcleo Regional de Ofiologia, universidade federal do Ceará (NuROf-ufC) Campus do Pici, Bloco 905, CEP , fortaleza - CE, Brazil; 3) Centro de investigação da Biodiversidade e Recursos Genéticos (CiBiO). universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando quintas, CEP , Vairão, Portugal. first record of bluish Podarcis muralis (LAuRENTi, 1768) Lizard coloration depends on the combined action of three classes of pigment or light-reflecting cells (i.e., chromatophores) located in the dermal layer of the skin. The xantophores are the most superficial and contain pigments (i.e., pteridines and/or caro - tenoids) that absorb short-wavelength light and reflect long wavelengths. iridophores contain intracellular guanine platelets that scatter the incident light. Melanophores oc - cupy a basal position in the dermis and contain eumelanin that absorbs all light transmitted by the xantophores and the irido - phores (COOPER & GREENBERG 2002; GRE - THER et al. 2004). Variation in the abundance and in the spatial arrangement of these cell types can produce the great array of skin colors found in lizards (e.g., SAENkO et al. 2013). Blue coloration has long attracted the attention of researchers and herpetoculturists due, among other reasons, to the fact that blue pigments are almost absent in nature (BAGNARA 2007; umbers 2013; but see GODA & fuji 1995). in vertebrates, blue is generally thought to be a structural color that results from selective light scattering by nanoscale elements that differ in refractive index (BAGNARA 2007). in particular, shortwavelength colors (blue and ultravioletblue) in lizards are structural colors produced by light scattering in the iridophores, although these colors also depend on interactions with xanthophores and the underlying layer of melanophores (MENTER et al. 1979; kuriyama et al. 2006; BAGNARA et al. 2007). in Anolis carolinensis VOiGT, 1832, the isolated iridophores appear blue-green under reflected light, and the color intensifies if a layer of melanophores is added under the iridophores (presumably due to absorption of longer wavelengths, ROHR - LiCH & PORTER 1972; ROHRLiCH 1974). The addition of xanthophores containing yellow pigments results in the normal brown-green skin color of the species. These results suggest that blue colors are produced when xanthophores contain few or no pigments (allowing almost all wavelengths of the incident light to interact directly with the iridophores). Consequently, the term axan-