Acinetobacter sp. isolates from emergency departments in two hospitals of South Korea
|
|
- Harvey Rodgers
- 5 years ago
- Views:
Transcription
1 Journal of Medical Microbiology (2014), 63, DOI /jmm Acinetobacter sp. isolates from emergency departments in two hospitals of South Korea Ji-Young Choi, 1 3 Eun Ah Ko, 2 3 Ki Tae Kwon, 3 Shinwon Lee, 3 Choel In Kang, 4 Doo-Ryeon Chung, 4 Kyong Ran Peck, 4 Jae-Hoon Song 4,5 and Kwan Soo Ko 1,5 Correspondence Kwan Soo Ko ksko@skku.edu 1 Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea 2 Korean Minjok Leadership Academy, Heongseong, Republic of Korea 3 Division of Infectious Diseases, Daegu Fatima Hospital, Daegu, Republic of Korea 4 Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea 5 Asia Pacific Foundation for Infectious Diseases (APFID), Seoul, Republic of Korea Received 6 March 2014 Accepted 25 July 2014 A total of 114 Acinetobacter sp. isolates were collected from patients in the emergency departments (EDs) of two Korean hospitals. Most isolates belonged to the Acinetobacter baumannii complex (105 isolates, 92.1 %). Imipenem resistance was found in 39 isolates (34.2 %) of the Acinetobacter sp. isolates, and 6 colistin-resistant isolates were also identified. Species distribution and antimicrobial-resistance rates were different between the two hospitals. In addition, two main clones were identified in the imipenem-resistant A. baumannii isolates from hospital B, but very diverse and novel genotypes were found in those from hospital A. Many Acinetobacter sp. isolates, including the imipenem-resistant A. baumannii, are considered to be associated with the community. The evidence of high antimicrobial resistance and different features in these Acinetobacter sp. isolates between the two EDs suggests the need for continuous testing to monitor changes in epidemiology. INTRODUCTION Until the 1980s, Acinetobacter spp. were considered as merely colonizers and were often neglected in the clinical setting; however, this group has now become one of the most problematic healthcare-associated pathogens (Munoz- Price & Weinstein, 2008). In particular, the emergence of multidrug-resistant or even pandrug-resistant Acinetobacter baumannii isolates has become a serious clinical problem in many parts of the world. Although Acinetobacter spp. have been known to cause mainly nosocomial infections, particularly in immunocompromised patients and patients in intensive care units, their presence in reservoirs outside the hospital has been recently reported (Eveillard et al., 2013; Choi et al., 2012). In addition, community-acquired Acinetobacter infections, such as bacteraemia, pneumonia and cellulitis, which may usually be associated with a detrimental patient s condition or comorbidity, have been repeatedly reported (Henao-Martínez et al., 2012; Gaillard et al., 2012; 3These authors contributed equally to this work. Abbreviations: AGS, Acinetobacter genomic species; CLSI Clinical and Laboratory Standards Institute; ED, emergency department; MLST, multilocus sequence typing; ST, sequence type. Leung et al., 2006; Peng et al., 2012). More frequently, community-onset infections by antimicrobial-resistant Acinetobacter spp. have been identified, and Acinetobacter spp. are now becoming a challenge for clinicians in emergency departments (EDs) (Kang et al., 2012a, b). However, the study of Acinetobacter sp. characteristics using ED isolates has rarely been performed. In this study, we investigated species distribution, antimicrobial resistance and genotypes to identify differences in the epidemiology of Acinetobacter sp. isolates between the EDs of two hospitals in Korea. The two hospitals that participated in this study showed different features. While one hospital (hospital A) is located in Seoul, the capital of Korea, another hospital (hospital B) is located in Daegu, which is approximately 300 km away from Seoul. In addition, while hospital A is a tertiary-care hospital, hospital B is a secondary-care hospital. We identified some different characteristics of the Acinetobacter sp. isolates from the EDs between the two hospitals. METHODS Bacterial isolates and species identification. A total of 114 Acinetobacter sp. isolates from patients were collected from May G 2014 The Authors Printed in Great Britain 1363
2 J.-Y. Choi and others to May 2013 from two EDs in Korean hospitals: one (hospital A) is a tertiary care hospital located in Seoul, the capital of Korea (91 isolates), and the other (hospital B) is a secondary hospital located in the province of Daegu (23 isolates). The isolation sources are listed in Table 1. Only the first isolate from each patient was included in this study. Preliminary species identification as Acinetobacter sp. isolates was performed using the VITEK-2 system. Acinetobacter spp. were determined based on partial rpob gene sequences (468 bp), as described elsewhere (Ko et al., 2007; La Scola et al., 2006). In vitro antimicrobial-susceptibility testing. In vitro antimicrobial-susceptibility testing was performed with all Acinetobacter sp. isolates by measuring MIC using the broth microdilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines (CLSI, 2013). Eleven antimicrobial agents were tested: imipenem, colistin, ampicillin/sulbactam, cefotaxime, ceftazidime, ciprofloxacin, gentamicin, rifampicin, trimethoprim/sulfamethoxazole, tetracycline and tigecycline. CLSI susceptibility interpretive criteria were used (CLSI, 2013). Because no breakpoints for rifampicin and tigecycline are available in the CLSI guidelines, the CLSI criteria recommended for staphylococci were applied for rifampicin (resistant 4 mgl 21 ) (CLSI, 2013), and the criteria of the United States Food and Drug Administration for Enterobacteriaceae were used for tigecycline (intermediate 4 mg l 21 and resistant 8 mgl 21 ). Escherichia coli ATCC 25922, Staphylococcus aureus ATCC and Pseudomonas aeruginosa ATCC were used as control strains. Multilocus sequence typing (MLST), PFGE and antimicrobialresistance genes. MLST was performed for 28 imipenem-resistant A. baumannii isolates, as described elsewhere (Bartual et al., 2005). All MLST data, including newly identified alleles and sequence types (STs), were submitted to the A. baumannii MLST database ( pubmlst.org/abaumannii). A phylogenetic tree was reconstructed based on concatenated sequences of seven housekeeping genes using the neighbour-joining method. For PFGE, genomic DNA was digested using the ApaI restriction enzyme. The restriction fragments were separated by PFGE using a temperature-controlled CHEF DR III system (Bio-Rad). For the 39 imipenem-resistant Acinetobacter sp. isolates, resistance genes, including bla IMP, bla VIM, bla GIM, bla SIM, bla KPC, bla NDM-1 and bla OXA-48, as well as bla OXA-23-like, bla OXA-24-like, bla OXA-51-like and bla OXA-58-like, were also detected by PCR, as described elsewhere (Lee et al., 2005). RESULTS A total of 105 out of 114 Acinetobacter sp. isolates from the EDs belonged to the A. baumannii complex [or Acinetobacter calcoaceticus A. baumannii (Acb) complex], including A. baumannii, A. pittii, A. nosocomialis, Acinetobacter genomic species (AGS) close to 13TU and A. calcoaceticus (105 isolates, 92.1 %) (Table 1). Of these, A. baumannii was the most frequently isolated species (72 isolates, 63.2 %), and it was more frequently isolated in hospital B (20 out of 23 isolates, 87.0 %) than in hospital A (52 out of 91 isolates, 57.1 %). A. pittii was the second most common species (14 isolates, 12.3 %), followed by A. nosocomialis (11 isolates, 9.6 %), A. calcoaceticus (5 isolates, 4.4 %), A. soli (5 isolates, 4.4 %) and AGS close to 13TU (3 isolates, 2.6 %). Other species (Acinetobacter johnsonii, A. junii, A. ursingii and AGS 9) were represented by one isolate. A. pittii, A. soli, A. johnsonii, A. junii, A. ursingii and AGS 9 were identified only in hospital A. Among the 18 Acinetobacter sp. blood isolates, only six isolates (33.3 %) were classified as A. baumannii (Table 1). The imipenem-resistance rate was 34.2 % (39 isolates) among the Acinetobacter sp. isolates (Table 2). It was lower in A. pittii (2 out of 14 isolates, 14.3 %) than in other species. Six colistin-resistant Acinetobacter sp. isolates were identified: three belonged to A. baumannii, and one each to A. nosocomialis, A. calcoaceticus and A. soli. Resistance rates to the other antimicrobial agents among the Acinetobacter sp. isolates ranged from 22.8 to 53.3 %: 38.6 % for ampicillin/sulbactam, 42.1 % for cefotaxime, 53.5 % for ceftazidime, 48.2 % for ciprofloxacin, 43.9 % for gentamicin, 50.9 % for rifampicin and 43.0 % for trimethoprim/ sulfamethoxazole (Table 2). For tigecycline, 16 isolates (14.0 %) of Acinetobacter sp. and 13 isolates (18.1 %) of A. baumannii showed tigecycline intermediate susceptibility or were resistant (MIC 4 mgl 21 ) (Table 2). Of these, Table 1. Species and sources of Acinetobacter sp. isolates from the EDs of two Korean hospitals Species No. (%) Source SP BL UR Bile Pus TA DS PTF CSF AB OS PF UK A. baumannii 72 (63.2) A. pittii 14 (12.3) A. nosocomialis 11 (9.6) A. calcoaceticus 5 (4.4) AGS close to 13TU 3 (2.6) A. soli 5 (4.4) A. johnsonii 1 (0.9) 1 A. junii 1 (0.9) 1 A. ursingii 1 (0.9) 1 AGS 9 1 (0.9) 1 Total 114 (100) AB, Abscess; BL, blood; CSF, cerebrospinal fluid; DS, dialysate; OS, oral swab; PF, pleural fluid; PTF, peritoneal fluid; SP, sputum; TA, tracheal aspirate; UK, unknown; UR, urine Journal of Medical Microbiology 63
3 Acinetobacter from emergency departments Table 2. Antimicrobial-resistance rates in Acinetobacter sp. isolates from the EDs of two Korean hospitals Species (no. of isolates) Resistance rate (%) Hospital A Hospital B IMI CL A/S CTX CAZ CIP GEN RIF SXT TET TIG* IMI CL A/S CTX CAZ CIP GEN RIF SXT TET TIG* Total (A, 91; B, 23) A. baumannii complex (A, 82; B, 23) A. baumannii (A, 52; B, 20) A. pittii (A, 14; B, 0) A. nosocomialis (A, 10; B, 1) A. calcoaceticus (A, 4; B, 1) A. soli (A, 5; B, 0) A/S, Ampicillin/sulbactam; CAZ, ceftazidime; CIP, ciprofloxacin; CL, colistin; CTX, cefotaxime; GEN, gentamicin; IMI, imipenem; RIF, rifampicin; SXT, trimethoprim/sulfamethoxazole; TET, tetracycline; TIG, tigecycline. *For tigecycline, nonsusceptibility (resistance and intermediate susceptibility) is shown. five isolates (four A. baumannii and one A. pittii) were resistant to tigecycline (MIC 8 mgl 21 ). Antimicrobial-resistance rates differed between Acinetobacter sp. isolates from hospitals A and B. While 16 out of 23 of Acinetobacter sp. isolates (69.6 %) from hospital B were resistant to imipenem, only 23 out of 91 isolates (25.3 %) from hospital A were imipenem-resistant. For colistin, 4.4 and 8.7 % Acinetobacter sp. isolates from hospitals A and B were resistant, respectively. While Acinetobacter sp. isolates from hospital A showed low resistance rates for ampicillin/sulbactam, cefotaxime, ceftazidime, ciprofloxacin, gentamicin, rifampicin, trimethoprim/sulbactam and tetracycline, those from hospital B showed higher resistance rates for them. While 12.1 % of Acinetobacter sp. isolates from hospital A were nonsusceptible to tigecycline, 21.7 % from hospital B were tigecycline nonsusceptible. The presence of antimicrobial-resistance genes was tested for the 39 imipenem-resistant Acinetobacter sp. isolates. Although the bla OXA-51-like gene and its upstream insertion element, ISAba1, were identified in all imipenem-resistant A. baumannii isolates, they were also found in one imipenem-resistant A. calcoaceticus pus isolate from hospital A. The bla OXA-23-like gene was found only in A. baumannii isolates. Among the 28 imipenem-resistant A. baumannii isolates, the bla OXA-23-like gene was identified in 19 isolates (67.9 %). While all 15 imipenem-resistant A. baumannii isolates from hospital B contained the bla OXA-23-like gene, the bla OXA-23-like gene was found in only 3 out of 13 imipenem-resistant A. baumannii isolates from hospital A (23.1 %). The other resistance genes tested in this study were not identified in any imipenem-resistant Acinetobacter sp. isolates. In imipenem-resistant A. baumannii isolates, in which the carbapenemase genes were not detected except for bla OXA-51-like, it is expected that efflux pumps may play a role in imipenem resistance. In addition, the bla OXA-51-like gene may also contribute the imipenem resistance because all isolates have ISAba1 elements upstream of bla OXA-51-like. In MLST analysis, 15 STs were identified among the 28 imipenem-resistant A. baumannii isolates (Table 3). Of these, 11 STs were newly identified in this study. CC92 including six STs was identified, and ST767 and ST774 comprised a CC. However, the other STs constituted singletons. ST357 and ST138 were represented by eight and seven isolates, respectively, but the other 13 STs were represented by only one isolate. All but one of the isolates belonging to ST357 and ST138 were from hospital B. Thus, all but one imipenem-resistant A. baumannii isolates from hospital B belonged to at least one of two main STs. ST357 and ST138 imipenem-resistant A. baumannii isolates showed different restriction fragment patterns in PFGE analysis from each other, but a very similar PFGE pattern was found within the same ST (data not shown). In contrast, no imipenem-resistant A. baumannii isolates from hospital A were represented by the same ST. In
4 J.-Y. Choi and others Table 3. Genotypes of imipenem-resistant A. baumannii isolates in MLST analysis Genotype Allelic profile* No. of isolates addition, 13 different STs from hospital A showed multiple nucleotide differences, and a phylogenetic tree from concatenated nucleotide sequences of the 7 gene fragments confirmed multiple origins of the imipenem-resistant A. baumannii isolates from hospital A (Fig. 1). DISCUSSION Hospital A (n513) Hospital B (n515) CC92 ST ST ST ST ST ST Others ST ST ST ST ST ST ST ST ST *glta-gyrb-gdhb-reca-cpn60-gpi-rpod. Several recent studies have reported community-onset Acinetobacter infections (Henao-Martínez et al., 2012; Gaillard et al., 2012; Leung et al., 2006; Peng et al., 2012), and have suggested the association of community-based A. baumannii isolates with these infections rather than nosocomial strains (Eveillard et al., 2013; Choi et al., 2012). In addition, clinical features of A. baumannii infections in patients admitted to EDs have been investigated in Korea (Kang et al., 2012a, b). In this study, we collected Acinetobacter sp. isolates from two EDs in Korea, and their characteristics were investigated. Although the different number of isolates and different locality of the two hospitals would be a limitation of this study, we identified that A. baumannii is the most prevalent species in the EDs, as in nosocomial infections. In our previous studies, A. baumannii constituted % of Acinetobacter sp. isolates in Korea (Ko et al., 2007; Park et al., 2012). However, the identification of A. soli had not been reported in the previous studies. A. soli was recently characterized as a novel species in Korea (Kim et al., 2008). Although it was first identified and isolated from forest soil, it has been reported to cause bloodstream infections, and carbapenem-resistant A. soli isolates have been identified (Pellegrino et al., 2011; Endo et al., 2012). In this study, we identified five A. soli isolates from one ED. Of these, two were isolated from the blood, and two were highly resistant to imipenem (MICs.64 mg l 21 ). In particular, one A. soli dialysate isolate (SMC ) was resistant to colistin and susceptible only to tigecycline. It is considered that antimicrobial-resistant A. soli, which is ubiquitous in the environment, can cause human infection, and thus should be included as an antimicrobial-resistant human pathogen. It is noted that many Acinetobacter sp. isolates were antimicrobial resistant. In particular, one-third of the Acinetobacter sp. isolates were resistant to imipenem. In a previous study on Acinetobacter sp. isolates collected in two hospitals from 2002 to 2006 in Korea, only 8.3 % were imipenem resistant (Ko et al., 2007). We have reported a drastic increase in imipenem resistance in Acinetobacter sp. isolates causing bloodstream infections in Korea from 15.9 % in to 77.6 % in (Park et al., 2012). Our results in this study are consistent with the increase of imipenem-resistant Acinetobacter sp. isolates in hospitals. In addition, we identified six colistin-resistant isolates, and the tigecycline-nonsusceptibility rate was 14.0 %. Thus, antimicrobial resistance should be not ignored in Acinetobacter sp. isolates from EDs in Korea. However, it should be noted that species distribution and antimicrobial-resistance rates were significantly different between the two hospitals. While most Acinetobacter sp. isolates belonged to A. baumannii and only three nonbaumannii Acinetobacter sp. isolates were identified in hospital B, ten species were identified and 57.1 % of isolates from the ED were classified as A. baumannii in hospital A. Resistance rates for most antimicrobial agents were higher in Acinetobacter sp. isolates from hospital B than in those from hospital A. In addition, clonality of the imipenemresistant A. baumannii isolates was also different between the two hospitals. While all but one of the imipenemresistant A. baumannii isolates from hospital B belonged to the ST138 and ST357 clones, no clonality was identified among the imipenem-resistant A. baumannii isolates from hospital A. In addition, most of the bla OXA-23-like genes, which are commonly related to carbapenem resistance worldwide, were identified in imipenem-resistant A. baumannii isolates from hospital B located in the Daegu province. That is, clonal A. baumannii isolates with high antimicrobial resistance might be brought into the hospital through the ED in hospital B, but not in hospital A. Such differences may be due to the features of the hospitals. Differences in region and hospital level may affect the features of the EDs and their patients, resulting in a difference in pathogens. Thus, understanding the characteristics of pathogens in each hospital will be important for the prevention, control and treatment of infections. ST357, one of the main clones identified in hospital B, was not identified in A. baumannii isolates causing nosocomial 1366 Journal of Medical Microbiology 63
5 Acinetobacter from emergency departments ST779 (07AC-343) ST778 (SMC ) ST768 (10AC-023) ST75 (07AC-306) ST773 (SMC ) ST780 (FA434) CC92 ST138 (SMC , FA127, FA330, FA336, FA475, FA763, FA789) ST769 (SMC ) ST770 (SMC ) ST357 (FA425, FA426, FA765, FA593, FA790, FA833, FA890, FA947) ST598 (07AC-256) ST774 (SMC ) ST767 (07AC-369) ST766 (07AC-018) ST771 (SMC ) Fig. 1. Unrooted phylogenetic tree of 28 imipenem-resistant A. baumannii isolates (15 STs) as reconstructed based on concatenated sequences of 7 housekeeping genes used in MLST. The tree was generated by the neighbour-joining method. Isolates from hospital A are underlined, and CC92 is also represented. Bar indicates 1 substitution per 1000 nt. infections in Korea (Park et al., 2012). It was speculated that new antimicrobial-resistant A. baumannii clones may be imported into the hospital from the community and can then spread within the hospital. In addition, most of the imipenem-resistant A. baumannii isolate genotypes from hospital A have not been previously identified. Thus, these isolates may also be imported from the community, but have not been spread within the hospital. However, identification of ST138, which belonged to CC92 or the global clone 2 distributed in Korea (Kim et al., 2013), as a main clone of imipenem-resistant A. baumannii in the ED suggests different implications. In our study, we identified that ST138 increased recently in a Korean hospital (Park et al., 2012). The ST138 clone identified from the ED in this study may be not be community based or may disperse into the community due to the blurred boundary between the hospital and the community. In this study, we identified antimicrobial-resistant Acinetobacter sp. isolates from two EDs in Korea. The two hospitals showed different characteristics of Acinetobacter sp. isolates, such as species distribution, antimicrobial resistance and clonality of imipenem-resistant A. baumannii isolates. Thus, initial antimicrobial coverage of Acinetobacter infections in EDs should be considered in each hospital. In addition, many Acinetobacter sp. isolates including imipenem-resistant A. baumannii are supposed to be associated with the community. Continuous surveillance studies are warranted to monitor changes in the epidemiology of hospital and community-onset Acinetobacter sp. infections. ACKNOWLEDGEMENTS This study was performed partly by E. A. Ko as her internship program at Sungkyunkwan University School of Medicine. This research was supported in part by the Basic Science Program through the National Research Foundation of Korea (NRF) and was funded by the Ministry of Science, ICT and Future Planning (NRF-2013R1A2A2A ). REFERENCES Bartual, S. G., Seifert, H., Hippler, C., Luzon, M. A., Wisplinghoff, H. & Rodríguez-Valera, F. (2005). Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol 43,
6 J.-Y. Choi and others Choi, J. Y., Kim, Y., Ko, E. A., Park, Y. K., Jheong, W. H., Ko, G. P. & Ko, K. S. (2012). Acinetobacter species isolates from a range of environments: species survey and observations of antimicrobial resistance. Diagn Microbiol Infect Dis 74, CLSI (2013). Performance Standards for Antimicrobial Susceptibility Testing; 23rd Informational Supplement M100 S23. Wayne, PA: Clinical and Laboratory Standards Institute. Endo, S., Sasano, M., Yano, H., Arai, K., Aoyagi, T., Hatta, M., Gu, Y., Yamada, M., Tokuda, K. & other authors (2012). First carbapenemresistant isolates of Acinetobacter soli in Japan. Antimicrob Agents Chemother 56, Eveillard, M., Kempf, M., Belmonte, O., Pailhoriès, H. & Joly-Guillou, M. L. (2013). Reservoirs of Acinetobacter baumannii outside the hospital and potential involvement in emerging human communityacquired infections. Int J Infect Dis 17, e802 e805. Gaillard, T., Darles, C., Pons, S., Martinaud, C., Soler, C. & Brisou, P. (2012). Acinetobacter parvus bacteraemia community-acquired. Int J Med Microbiol 302, Henao-Martínez, A. F., González-Fontal, G. R. & Johnson, S. (2012). A case of community-acquired Acinetobacter junii-johnsonii cellulitis. Biomedica 32, Kang, C.-I., Chung, D. R., Peck, K. R., Song, J.-H. & Korean Network for Study on Infectious Diseases (KONSID) (2012a). Clinical predictors of Pseudomonas aeruginosa or Acinetobacter baumannii bacteremia in patients admitted to the ED. Am J Emerg Med 30, Kang, S. J., Kang, C.-I., Park, S. Y., Ha, Y. E., Joo, E.-J., Chung, D. R., Peck, K. R., Lee, N. Y. & Song, J.-H. (2012b). Epidemiology and clinical features of community-onset Acinetobacter baumannii infections. Infect Control Hosp Epidemiol 33, Kim, D., Baik, K. S., Kim, M. S., Park, S. C., Kim, S. S., Rhee, M. S., Kwak, Y. S. & Seong, C. N. (2008). Acinetobacter soli sp. nov., isolated from forest soil. J Microbiol 46, Kim, D. H., Choi, J. Y., Kim, H. W., Kim, S. H., Chung, D. R., Peck, K. R., Thamlikitkul, V., So, T. M. K., Yasin, R. M. D. & other authors (2013). Spread of carbapenem-resistant Acinetobacter baumannii global clone 2 in Asia and AbaR-type resistance islands. Antimicrob Agents Chemother 57, Ko, K. S., Suh, J. Y., Kwon, K. T., Jung, S. I., Park, K. H., Kang, C. I., Chung, D. R., Peck, K. R. & Song, J.-H. (2007). High rates of resistance to colistin and polymyxin B in subgroups of Acinetobacter baumannii isolates from Korea. J Antimicrob Chemother 60, La Scola, B., Gundi, V. A., Khamis, A. & Raoult, D. (2006). Sequencing of the rpob gene and flanking spacers for molecular identification of Acinetobacter species. J Clin Microbiol 44, Lee, K., Yum, J. H., Yong, D., Lee, H. M., Kim, H. D., Docquier, J. D., Rossolini, G. M. & Chong, Y. (2005). Novel acquired metallo-blactamase gene, bla SIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob Agents Chemother 49, Leung, W. S., Chu, C. M., Tsang, K. Y., Lo, F. H., Lo, K. F. & Ho, P. L. (2006). Fulminant community-acquired Acinetobacter baumannii pneumonia as a distinct clinical syndrome. Chest 129, Munoz-Price, L. S. & Weinstein, R. A. (2008). Acinetobacter infection. N Engl J Med 358, Park, Y. K., Jung, S. I., Park, K. H., Kim, D. H., Choi, J. Y., Kim, S. H. & Ko, K. S. (2012). Changes in antimicrobial susceptibility and major clones of Acinetobacter calcoaceticus-baumannii complex isolates from a single hospital in Korea over 7 years. J Med Microbiol 61, Pellegrino, F. L., Vieira, V. V., Baio, P. V., dos Santos, R. M., dos Santos, A.L.,Santos,N.G.,Meohas,M.M.,Santos,R.T.,deSouza,T.C.&other authors (2011). Acinetobacter soli as a cause of bloodstream infection in a neonatal intensive care unit. J Clin Microbiol 49, Peng, C., Zong, Z. & Fan, H. (2012). Acinetobacter baumannii isolates associated with community-acquired pneumonia in West China. Clin Microbiol Infect 18, E491 E Journal of Medical Microbiology 63
Differences in phenotypic and genotypic traits against antimicrobial agents between Acinetobacter baumannii and Acinetobacter genomic species 13TU
Journal of Antimicrobial Chemotherapy (2007) 59, 633 639 doi:10.1093/jac/dkm007 Advance Access publication 6 March 2007 Differences in phenotypic and genotypic traits against antimicrobial agents between
More informationPrevalence of Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa and its antibiogram in a tertiary care centre
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 952-956 http://www.ijcmas.com Original Research Article Prevalence of Metallo-Beta-Lactamase
More informationOriginal Article Clinical Microbiology
Original Article Clinical Microbiology Ann Lab Med 2017;37:231-239 https://doi.org/10.3343/alm.2017.37.3.231 ISSN 2234-3806 eissn 2234-3814 Increasing Resistance to Extended-Spectrum Cephalosporins, Fluoroquinolone,
More informationMETHODS. Imipenem Meropenem Colistin Polymyxin B Ampicillinsulbactam. Downloaded from by IP:
Journal of Medical Microbiology (01), 1, 353 30 DOI.99/jmm.0.03939-0 In vitro time-kill studies of antimicrobial agents against blood isolates of imipenem-resistant Acinetobacter baumannii, including colistin-
More informationESCMID elibrary. Symposium: Acinetobacter Infections from East to West. Molecular Epidemiology Worldwide
Symposium: Acinetobacter Infections from East to West Molecular Epidemiology Worldwide Harald Seifert Institut für Medizinische Mikrobiologie, Immunologie und Hygiene der Universität zu Köln 26 th ECCMID,
More informationPrinciples of Antimicrobial Therapy
Principles of Antimicrobial Therapy Doo Ryeon Chung, MD, PhD Professor of Medicine, Division of Infectious Diseases Director, Infection Control Office SUNGKYUNKWAN UNIVERSITY SCHOOL OF MEDICINE CASE 1
More informationWitchcraft for Gram negatives
Witchcraft for Gram negatives Dr Subramanian S MD DNB MNAMS AB (Medicine, Infect Dis) Infectious Diseases Consultant Global Health City, Chennai www.asksubra.com Drug resistance follows the drug like a
More informationOutline. Antimicrobial resistance. Antimicrobial resistance in gram negative bacilli. % susceptibility 7/11/2010
Multi-Drug Resistant Organisms Is Combination Therapy the Way to Go? Sutthiporn Pattharachayakul, PharmD Prince of Songkhla University, Thailand Outline Prevalence of anti-microbial resistance in Acinetobacter
More informationWhat does multiresistance actually mean? Yohei Doi, MD, PhD University of Pittsburgh
What does multiresistance actually mean? Yohei Doi, MD, PhD University of Pittsburgh Disclosures Merck Research grant Clinical context of multiresistance Resistance to more classes of agents Less options
More informationSeasonal and Temperature-Associated Increase in Community-Onset Acinetobacter baumannii Complex Colonization or Infection
Brief Communication Clinical Microbiology Ann Lab Med 18;38:266-27 https://doi.org/.3343/alm.18.38.3.266 ISSN 2234-386 eissn 2234-3814 Seasonal and Temperature-Associated Increase in Community-Onset Acinetobacter
More informationAntimicrobial Cycling. Donald E Low University of Toronto
Antimicrobial Cycling Donald E Low University of Toronto Bad Bugs, No Drugs 1 The Antimicrobial Availability Task Force of the IDSA 1 identified as particularly problematic pathogens A. baumannii and
More informationFlorida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC
Florida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC 11/20/2014 1 To describe carbapenem-resistant Enterobacteriaceae. To identify laboratory detection standards for carbapenem-resistant
More informationSurveillance of Antimicrobial Resistance among Bacterial Pathogens Isolated from Hospitalized Patients at Chiang Mai University Hospital,
Original Article Vol. 28 No. 1 Surveillance of Antimicrobial Resistance:- Chaiwarith R, et al. 3 Surveillance of Antimicrobial Resistance among Bacterial Pathogens Isolated from Hospitalized Patients at
More informationDiversity in Acinetobacter baumannii isolates from paediatric cancer patients in Egypt
ORIGINAL ARTICLE BACTERIOLOGY Diversity in Acinetobacter baumannii isolates from paediatric cancer patients in Egypt L. Al-Hassan 1, H. El Mehallawy 2 and S.G.B. Amyes 1 1) Medical Microbiology, University
More informationEARS Net Report, Quarter
EARS Net Report, Quarter 4 213 March 214 Key Points for 213* Escherichia coli: The proportion of patients with invasive infections caused by E. coli producing extended spectrum β lactamases (ESBLs) increased
More informationUpdate on Resistance and Epidemiology of Nosocomial Respiratory Pathogens in Asia. Po-Ren Hsueh. National Taiwan University Hospital
Update on Resistance and Epidemiology of Nosocomial Respiratory Pathogens in Asia Po-Ren Hsueh National Taiwan University Hospital Ventilator-associated Pneumonia Microbiological Report Sputum from a
More informationAcinetobacter Outbreaks: Experience from a Neurosurgery Critical Care Unit. Jumoke Sule Consultant Microbiologist 19 May 2010
Acinetobacter Outbreaks: Experience from a Neurosurgery Critical Care Unit Jumoke Sule Consultant Microbiologist 19 May 2010 Epidemiology of Acinetobacter spp At least 32 different species Recovered from
More informationDR. MICHAEL A. BORG DIRECTOR OF INFECTION PREVENTION & CONTROL MATER DEI HOSPITAL - MALTA
DR. MICHAEL A. BORG DIRECTOR OF INFECTION PREVENTION & CONTROL MATER DEI HOSPITAL - MALTA The good old days The dread (of) infections that used to rage through the whole communities is muted Their retreat
More informationNon-Susceptibility of Bacterial Pathogens Causing Hospital-Onset Pneumonia UK and Ireland,
Non-Susceptibility of Bacterial Pathogens Causing Hospital-Onset Pneumonia UK and Ireland, 2008-2016 Alicia Russell Federation of Infection Societies conference 14 th November 2018 alisia_russell BSAC
More information2016/LSIF/FOR/007 Improving Antimicrobial Use and Awareness in Korea
2016/LSIF/FOR/007 Improving Antimicrobial Use and Awareness in Korea Submitted by: Asia Pacific Foundation for Infectious Diseases Policy Forum on Strengthening Surveillance and Laboratory Capacity to
More informationMolecular epidemiology of Acinetobacter baumannii and Acinetobacter nosocomialis in Germany over a 5-year period ( )
ORIGINAL ARTICLE 10.1111/1469-0691.12026 Molecular epidemiology of Acinetobacter baumannii and Acinetobacter nosocomialis in Germany over a 5-year period (2005 2009) X. Schleicher 1, P. G. Higgins 1, H.
More informationMulti-drug resistant Acinetobacter (MDRA) Surveillance and Control. Alison Holmes
Multi-drug resistant Acinetobacter (MDRA) Surveillance and Control Alison Holmes The organism and it s epidemiology Surveillance Control What is it? What is it? What is it? What is it? Acinetobacter :
More informationIntrinsic, implied and default resistance
Appendix A Intrinsic, implied and default resistance Magiorakos et al. [1] and CLSI [2] are our primary sources of information on intrinsic resistance. Sanford et al. [3] and Gilbert et al. [4] have been
More informationAcinetobacter Resistance in Turkish Tertiary Care Hospitals. Zeliha KOCAK TUFAN, MD, Assoc. Prof.
Acinetobacter Resistance in Turkish Tertiary Care Hospitals Zeliha KOCAK TUFAN, MD, Assoc. Prof. Acinetobacter Problem Countries that have reported hospital outbreaks of carbapenem-resistant Acinetobacter
More informationMolecular Epidemiology and Insights into the Genomes of. Acinetobacter calcoaceticus - Acinetobacter baumannii complex
Molecular Epidemiology and Insights into the Genomes of Acinetobacter calcoaceticus - Acinetobacter baumannii complex Witchuda Kamolvit MD A thesis submitted for the degree of Doctor of Philosophy at The
More informationFighting MDR Pathogens in the ICU
Fighting MDR Pathogens in the ICU Dr. Murat Akova Hacettepe University School of Medicine, Department of Infectious Diseases, Ankara, Turkey 1 50.000 deaths each year in US and Europe due to antimicrobial
More informationActivity of a novel aminoglycoside, ACHN-490, against clinical isolates of Escherichia coli and Klebsiella pneumoniae from New York City
Journal of Antimicrobial Chemotherapy Advance Access published July 31, 2010 J Antimicrob Chemother doi:10.1093/jac/dkq278 Activity of a novel aminoglycoside, ACHN-490, against clinical isolates of Escherichia
More informationTHE NAC CHALLENGE PANEL OF ISOLATES FOR VERIFICATION OF ANTIBIOTIC SUSCEPTIBILITY TESTING METHODS
THE NAC CHALLENGE PANEL OF ISOLATES FOR VERIFICATION OF ANTIBIOTIC SUSCEPTIBILITY TESTING METHODS Stefanie Desmet University Hospitals Leuven Laboratory medicine microbiology stefanie.desmet@uzleuven.be
More informationMono- versus Bitherapy for Management of HAP/VAP in the ICU
Mono- versus Bitherapy for Management of HAP/VAP in the ICU Jean Chastre, www.reamedpitie.com Conflicts of interest: Consulting or Lecture fees: Nektar-Bayer, Pfizer, Brahms, Sanofi- Aventis, Janssen-Cilag,
More informationClinical and microbiological characterization of carbapenem-resistant Acinetobacter baumannii bloodstream infections
Journal of Medical Microbiology (2011), 60, 605 611 DOI 10.1099/jmm.0.029439-0 Clinical and microbiological characterization of carbapenem-resistant Acinetobacter baumannii bloodstream infections Joon
More informationAvailable online at
Available online at www.annclinlabsci.org Time-Kill Synergy Tests of Tigecycline Combined with Imipenem, Amikacin, and Ciprofloxacin against Clinical Isolates of Multidrug-Resistant Klebsiella pneumoniae
More informationAppropriate antimicrobial therapy in HAP: What does this mean?
Appropriate antimicrobial therapy in HAP: What does this mean? Jaehee Lee, M.D. Kyungpook National University Hospital, Korea KNUH since 1907 Presentation outline Empiric antimicrobial choice: right spectrum,
More informationThe Basics: Using CLSI Antimicrobial Susceptibility Testing Standards
The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards Janet A. Hindler, MCLS, MT(ASCP) UCLA Health System Los Angeles, California, USA jhindler@ucla.edu 1 Learning Objectives Describe information
More informationOvernight identification of imipenem-resistant Acinetobacter baumannii carriage in hospitalized patients
TABLE 1. Origin and carbapenem resistance characteristics of the 64 Acinetobacter baumannii stock D-750 Overnight identification of imipenem-resistant Acinetobacter baumannii carriage in hospitalized patients
More informationOriginal Article Clinical Microbiology INTRODUCTION
Original Article Clinical Microbiology Ann Lab Med 2016;36:124-130 http://dx.doi.org/10.3343/alm.2016.36.2.124 ISSN 2234-3806 eissn 2234-3814 In Vitro Interactions of Antibiotic Combinations of Colistin,
More informationAntimicrobial Resistance Strains
Antimicrobial Resistance Strains Microbiologics offers a wide range of strains with characterized antimicrobial resistance mechanisms including: Extended-Spectrum β-lactamases (ESBLs) Carbapenamases Vancomycin-Resistant
More informationEmerging antimicrobial resistance and clinical relevance of Acinetobacter isolates in a tertiary care hospital of rural area of Punjab, India
8 P a g e Emerging antimicrobial resistance and clinical relevance of Acinetobacter isolates in a tertiary care hospital of rural area of Punjab, India POONAM SHARMA 1, YOUSUF UL BASHIR 1, SARJIWAN KAUR
More informationPresenter: Ombeva Malande. Red Cross Children's Hospital Paed ID /University of Cape Town Friday 6 November 2015: Session:- Paediatric ID Update
Emergence of invasive Carbapenem Resistant Enterobacteriaceae CRE infection at RCWMCH Ombeva Oliver Malande, Annerie du Plessis, Colleen Bamford, Brian Eley Presenter: Ombeva Malande Red Cross Children's
More informationPROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains
PROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains 1 INTRODUCTION... 1 2 OBJECTIVES... 2 3 OUTLINE OF THE EQAS 2017... 2 3.1 Shipping, receipt and storage of strains...
More informationComparative Assessment of b-lactamases Produced by Multidrug Resistant Bacteria
Comparative Assessment of b-lactamases Produced by Multidrug Resistant Bacteria Juhee Ahn Department of Medical Biomaterials Engineering Kangwon National University October 23, 27 Antibiotic Development
More informationETX2514SUL (sulbactam/etx2514) for the treatment of Acinetobacter baumannii infections
ETX2514SUL (sulbactam/etx2514) for the treatment of Acinetobacter baumannii infections Robin Isaacs Chief Medical Officer, Entasis Therapeutics Dr. Isaacs is a full-time employee of Entasis Therapeutics.
More informationAerobic bacterial infections in a burns unit of Sassoon General Hospital, Pune
Original article Aerobic bacterial infections in a burns unit of Sassoon General Hospital, Pune Patil P, Joshi S, Bharadwaj R. Department of Microbiology, B.J. Medical College, Pune, India. Corresponding
More information2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose
2017 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility
More informationIn vitro Comparison of Anti-Biofilm Effects against
Original Article http://dx.doi.org/10.3947/ic.2015.47.1.27 Infect Chemother 2015;47(1):27-32 ISSN 2093-2340 (Print) ISSN 2092-6448 (Online) Infection & Chemotherapy In vitro Comparison of Anti-Biofilm
More information2015 Antibiogram. Red Deer Regional Hospital. Central Zone. Alberta Health Services
2015 Antibiogram Red Deer Regional Hospital Central Zone Alberta Health Services Introduction. This antibiogram is a cumulative report of the antimicrobial susceptibility rates of common microbial pathogens
More informationAbaR7, a Genomic Resistance Island Found in Multidrug-resistant Acinetobacter baumannii Isolates in Daejeon, Korea
Original Article Clinical Microbiology Ann Lab Med 2012;32:324-330 ISSN 2234-3806 eissn 2234-3814 AbaR7, a Genomic Resistance Island Found in Multidrug-resistant Acinetobacter baumannii Isolates in Daejeon,
More informationAntibacterial Resistance In Wales
A Report from Public Health Wales Antimicrobial Resistance Programme Surveillance Unit: Antibacterial Resistance In Wales 2005-2012 Authors: Maggie Heginbothom Robin Howe & Catherine Thomas Version: 1
More informationANTIMICROBIAL RESISTANCE SURVEILLANCE FROM SENTINEL PUBLIC HOSPITALS, SOUTH AFRICA, 2014
ANTIMICROBIAL RESISTANCE SURVEILLANCE FROM SENTINEL PUBLIC HOSPITALS, SOUTH AFRICA, 2014 Olga Perovic, 1,2 Verushka Chetty 1 & Samantha Iyaloo 1 1 National Institute for Communicable Diseases, NHLS 2 Department
More informationCharacterization of the Multidrug-Resistant Acinetobacter
Ann Clin Microbiol Vol. 7, No. 2, June, 20 http://dx.doi.org/0.55/acm.20.7.2.29 pissn 2288-0585 eissn 2288-6850 Characterization of the Multidrug-Resistant Acinetobacter species Causing a Nosocomial Outbreak
More informationReceived: February 29, 2008 Revised: July 22, 2008 Accepted: August 4, 2008
J Microbiol Immunol Infect. 29;42:317-323 In vitro susceptibilities of aerobic and facultative anaerobic Gram-negative bacilli isolated from patients with intra-abdominal infections at a medical center
More informationMDR Acinetobacter baumannii. Has the post antibiotic era arrived? Dr. Michael A. Borg Infection Control Dept Mater Dei Hospital Malta
MDR Acinetobacter baumannii Has the post antibiotic era arrived? Dr. Michael A. Borg Infection Control Dept Mater Dei Hospital Malta 1 The Armageddon recipe Transmissible organism with prolonged environmental
More informationMechanism of antibiotic resistance
Mechanism of antibiotic resistance Dr.Siriwoot Sookkhee Ph.D (Biopharmaceutics) Department of Microbiology Faculty of Medicine, Chiang Mai University Antibiotic resistance Cross-resistance : resistance
More informationCo-transfer of bla NDM-5 and mcr-1 by an IncX3 X4 hybrid plasmid in Escherichia coli 4
SUPPLEMENTARY INFORMATION ARTICLE NUMBER: 16176 DOI: 10.1038/NMICROBIOL.2016.176 Co-transfer of bla NDM-5 and mcr-1 by an IncX3 X4 hybrid plasmid in Escherichia coli 4 5 6 7 8 9 10 11 12 13 14 15 16 17
More informationThe impact of antimicrobial resistance on enteric infections in Vietnam Dr Stephen Baker
The impact of antimicrobial resistance on enteric infections in Vietnam Dr Stephen Baker sbaker@oucru.org Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam Outline The impact of antimicrobial
More informationESBL- and carbapenemase-producing microorganisms; state of the art. Laurent POIREL
ESBL- and carbapenemase-producing microorganisms; state of the art Laurent POIREL Medical and Molecular Microbiology Unit Dept of Medicine University of Fribourg Switzerland INSERM U914 «Emerging Resistance
More informationESCMID Online Lecture Library. by author
Expert rules in susceptibility testing EUCAST-ESGARS-EPASG Educational Workshop Linz, 16 19 September, 2014 Dr. Rafael Cantón Hospital Universitario Ramón y Cajal SERVICIO DE MICROBIOLOGÍA Y PARASITOLOGÍA
More informationMulti-drug resistant microorganisms
Multi-drug resistant microorganisms Arzu TOPELI Director of MICU Hacettepe University Faculty of Medicine, Ankara-Turkey Council Member of WFSICCM Deaths in the US declined by 220 per 100,000 with the
More information2016 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose
2016 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility
More informationMICRONAUT MICRONAUT-S Detection of Resistance Mechanisms. Innovation with Integrity BMD MIC
MICRONAUT Detection of Resistance Mechanisms Innovation with Integrity BMD MIC Automated and Customized Susceptibility Testing For detection of resistance mechanisms and specific resistances of clinical
More informationINCIDENCE OF BACTERIAL COLONISATION IN HOSPITALISED PATIENTS WITH DRUG-RESISTANT TUBERCULOSIS
INCIDENCE OF BACTERIAL COLONISATION IN HOSPITALISED PATIENTS WITH DRUG-RESISTANT TUBERCULOSIS 1 Research Associate, Drug Utilisation Research Unit, Nelson Mandela University 2 Human Sciences Research Council,
More information2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine
2012 ANTIBIOGRAM Central Zone Former DTHR Sites Department of Pathology and Laboratory Medicine Medically Relevant Pathogens Based on Gram Morphology Gram-negative Bacilli Lactose Fermenters Non-lactose
More informationInternational Journal of Antimicrobial Agents
International Journal of Antimicrobial Agents 33 (2009) 33 39 Contents lists available at ScienceDirect International Journal of Antimicrobial Agents journal homepage: http://www.elsevier.com/locate/ijantimicag
More informationAnalysis of community- and hospital-acquired bacteraemia during a recent 5-year period
Journal of Medical Microbiology (2014), 63, 421 426 DOI 10.1099/jmm.0.069054-0 Analysis of community- and hospital-acquired bacteraemia during a recent 5-year period Hee-Won Moon, Young Jin Ko, Seungman
More informationETX2514: Responding to the global threat of nosocomial multidrug and extremely drug resistant Gram-negative pathogens
ETX2514: Responding to the global threat of nosocomial multidrug and extremely drug resistant Gram-negative pathogens Ruben Tommasi, PhD Chief Scientific Officer ECCMID 2017 April 24, 2017 Vienna, Austria
More informationNosocomial Infections: What Are the Unmet Needs
Nosocomial Infections: What Are the Unmet Needs Jean Chastre, MD Service de Réanimation Médicale Hôpital Pitié-Salpêtrière, AP-HP, Université Pierre et Marie Curie, Paris 6, France www.reamedpitie.com
More informationInt.J.Curr.Microbiol.App.Sci (2017) 6(3):
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 3 (2017) pp. 891-895 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.603.104
More informationDetecting / Reporting Resistance in Nonfastidious GNR Part #2. Janet A. Hindler, MCLS MT(ASCP)
Detecting / Reporting Resistance in Nonfastidious GNR Part #2 Janet A. Hindler, MCLS MT(ASCP) Methods Described in CLSI M100-S21 for Testing non-enterobacteriaceae Organism Disk Diffusion MIC P. aeruginosa
More informationRETROSPECTIVE STUDY OF GRAM NEGATIVE BACILLI ISOLATES AMONG DIFFERENT CLINICAL SAMPLES FROM A DIAGNOSTIC CENTER OF KANPUR
Original article RETROSPECTIVE STUDY OF GRAM NEGATIVE BACILLI ISOLATES AMONG DIFFERENT CLINICAL SAMPLES FROM A DIAGNOSTIC CENTER OF KANPUR R.Sujatha 1,Nidhi Pal 2, Deepak S 3 1. Professor & Head, Department
More informationProceedings of the 19th American Academy of Veterinary Pharmacology and Therapeutics Biennial Symposium
www.ivis.org Proceedings of the 19th American Academy of Veterinary Pharmacology and Therapeutics Biennial Symposium May 17-20, 2015 Fort Collins, CO, USA Reprinted in the IVIS website with the permission
More informationRESEARCH ARTICLE ANTIBIOGRAM
RESEARCH ARTICLE ANTIBIOGRAM OF ESCHERICHIA COLI, KLEBSIELLA PNEUMONIAE, AND KLEBSIELLA OXYTOCA FROM INVASIVE DISEASE CASES AT A TERTIARY CARE UNIVERSITY HOSPITAL IN THE CENTRAL REGION OF JAPAN FROM 2008
More informationClonal Diversity of Nosocomial Epidemic Acinetobacter baumannii Strains Isolated in Spain
JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 2011, p. 875 882 Vol. 49, No. 3 0095-1137/11/$12.00 doi:10.1128/jcm.01026-10 Copyright 2011, American Society for Microbiology. All Rights Reserved. Clonal Diversity
More informationNew Opportunities for Microbiology Labs to Add Value to Antimicrobial Stewardship Programs
New Opportunities for Microbiology Labs to Add Value to Antimicrobial Stewardship Programs Patrick R. Murray, PhD Senior Director, WW Scientific Affairs 2017 BD. BD, the BD Logo and all other trademarks
More information1 INTRODUCTION OBJECTIVES OUTLINE OF THE SALM/CAMP EQAS
PROTOCOL For antimicrobial susceptibility testing of Salmonella, Campylobacter and optional genotypic characterisation of AmpC-, ESBL- and carbapenemase-producing test strains 1 INTRODUCTION... 1 2 OBJECTIVES...
More informationcrossm Global Assessment of the Activity of Tigecycline against Multidrug-Resistant Gram-negative pathogens between
RESEARCH ARTICLE Clinical Science and Epidemiology crossm Global Assessment of the Activity of Tigecycline against Multidrug-Resistant Gram-Negative Pathogens between 2004 and 2014 as Part of the Tigecycline
More informationMain objectives of the EURL EQAS s
EQAS Enterococci, Staphylococci and E. coli EURL workshop, April, 11 Lourdes García Migura Main objectives of the EURL EQAS s To improve the comparability of antimicrobial susceptibility testing (AST)
More informationRevista da Sociedade Brasileira de Medicina Tropical 49(2): , Mar-Apr,
Revista da Sociedade Brasileira de Medicina Tropical 49():65-7, Mar-Apr, 6 http://dx.doi.org/.59/37-868-4-5 Major Article Over expression of AdeABC and AcrAB-TolC efflux systems confers tigecycline resistance
More informationIn Vivo Selection of Pan-Drug Resistant Acinetobacter baumannii during Antibiotic Treatment
Original Article http://dx.doi.org/10.3349/ymj.2015.56.4.928 pissn: 0513-5796, eissn: 1976-2437 Yonsei Med J 56(4):928-934, 2015 In Vivo Selection of Pan-Drug Resistant Acinetobacter baumannii during Antibiotic
More informationAntimicrobial Resistance Surveillance from sentinel public hospitals, South Africa, 2013
Antimicrobial Resistance Surveillance from sentinel public s, South Africa, 213 Authors: Olga Perovic 1,2, Melony Fortuin-de Smidt 1, and Verushka Chetty 1 1 National Institute for Communicable Diseases
More informationSource: Portland State University Population Research Center (
Methicillin Resistant Staphylococcus aureus (MRSA) Surveillance Report 2010 Oregon Active Bacterial Core Surveillance (ABCs) Office of Disease Prevention & Epidemiology Oregon Health Authority Updated:
More informationNational Surveillance of Antimicrobial Resistance in Pseudomonas aeruginosa Isolates Obtained from Intensive Care Unit Patients from 1993 to 2002
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Dec. 2004, p. 4606 4610 Vol. 48, No. 12 0066-4804/04/$08.00 0 DOI: 10.1128/AAC.48.12.4606 4610.2004 Copyright 2004, American Society for Microbiology. All Rights
More informationAcinetobacter species-associated infections and their antibiotic susceptibility profiles in Malaysia.
Biomedical Research 12; 23 (4): 571-575 ISSN 97-938X Scientific Publishers of India Acinetobacter species-associated infections and their antibiotic susceptibility profiles in Malaysia. Nazmul MHM, Jamal
More informationThe UK 5-year AMR Strategy - a brief overview - Dr Berit Muller-Pebody National Infection Service Public Health England
The UK 5-year AMR Strategy - a brief overview - Dr Berit Muller-Pebody National Infection Service Public Health England Chief Medical Officer - Annual Report 2013 Antimicrobial resistance poses catastrophic
More informationβ-lactams resistance among Enterobacteriaceae in Morocco 1 st ICREID Addis Ababa March 2018
β-lactams resistance among Enterobacteriaceae in Morocco 1 st ICREID Addis Ababa 12-14 March 2018 Antibiotic resistance center Institut Pasteur du Maroc Enterobacteriaceae (E. coli, Salmonella, ) S. aureus
More information2015 Antimicrobial Susceptibility Report
Gram negative Sepsis Outcome Programme (GNSOP) 2015 Antimicrobial Susceptibility Report Prepared by A/Professor Thomas Gottlieb Concord Hospital Sydney Jan Bell The University of Adelaide Adelaide On behalf
More informationChanging trends in clinical characteristics and antibiotic susceptibility of Klebsiella pneumoniae bacteremia
ORIGINAL ARTICLE Korean J Intern Med 2018;33:595-603 Changing trends in clinical characteristics and antibiotic susceptibility of Klebsiella pneumoniae Miri Hyun, Chang In Noh, Seong Yeol Ryu, and Hyun
More informationMolecular characterization of carbapenemase genes in Acinetobacter baumannii in China
Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China F. Fang 1 *, S. Wang 2 *, Y.X. Dang 3, X. Wang 3 and G.Q. Yu 3 1 The CT Room, Nanyang City Center Hospital, Nanyang,
More informationDoes Screening for MRSA Colonization Have A Role In Healthcare-Associated Infection Prevention Programs?
Does Screening for MRSA Colonization Have A Role In Healthcare-Associated Infection Prevention Programs? John A. Jernigan, MD, MS Division of Healthcare Quality Promotion Centers for Disease Control and
More informationAcinetobacter lwoffii h h
hh Acinetobacter lwoffii h h h h hh MBL Acinetobacter lwoffii MBL A. lwoffii MBL MBL Acinetobacter lwoffii hh Staphylococcus pseudintermedius Pseudomonas aeruginosa h Escherichia coli, hhh ABCD Ambler
More informationDefining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate Confirmation Testing
Infect Dis Ther (2015) 4:513 518 DOI 10.1007/s40121-015-0094-6 BRIEF REPORT Defining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate
More informationAvailable online at ISSN No:
Available online at www.ijmrhs.com ISSN No: 2319-5886 International Journal of Medical Research & Health Sciences, 2017, 6(4): 36-42 Comparative Evaluation of In-Vitro Doripenem Susceptibility with Other
More informationPresence of extended spectrum β-lactamase producing Escherichia coli in
1 2 Presence of extended spectrum β-lactamase producing Escherichia coli in wild geese 3 4 5 A. Garmyn* 1, F. Haesebrouck 1, T. Hellebuyck 1, A. Smet 1, F. Pasmans 1, P. Butaye 2, A. Martel 1 6 7 8 9 10
More informationEuropean Committee on Antimicrobial Susceptibility Testing
European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control as recommended by EUCAST Version 5.0, valid from 015-01-09 This document should be cited as "The
More informationAnalysis of drug-resistant gene detection of blaoxa-like genes from Acinetobacter baumannii
Analysis of drug-resistant gene detection of blaoxa-like genes from Acinetobacter baumannii D.K. Yang, H.J. Liang, H.L. Gao, X.W. Wang and Y. Wang Department of Infections, The First Affiliated Hospital
More informationMultidrug-Resistant Acinetobacter
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 9 (2017) pp. 1598-1603 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.609.196
More informationInternational Journal of Antimicrobial Agents
International Journal of Antimicrobial Agents 35 (2010) 227 234 Contents lists available at ScienceDirect International Journal of Antimicrobial Agents journal homepage: http://www.elsevier.com/locate/ijantimicag
More informationR. Uma Karthika, 1 R. Srinivasa Rao, 1 Suchismita Sahoo, 1 P. Shashikala, 2 Reba Kanungo, 2 S. Jayachandran 1 and K. Prashanth 1 INTRODUCTION
Journal of Medical Microbiology (2009), 58, 430 435 DOI 10.1099/jmm.0.002105-0 Phenotypic and genotypic assays for detecting the prevalence of metallo-b-lactamases in clinical isolates of Acinetobacter
More informationThe relevance of Gram-negative pathogens for public health situation in India
The relevance of Gram-negative pathogens for public health situation in India Dr. Sanjay Bhattacharya MD, DNB, DipRCPath, FRCPath, CCT (UK) Consultant Microbiologist Tata Medical Center www.tmckolkata.com
More informationAn Intrinsic Strain of Colistin-resistant Acinetobacter Isolated from a Japanese Patient
CASE REPORT An Intrinsic Strain of Colistin-resistant Acinetobacter Isolated from a Japanese Patient Etsuko Takizawa 1, Koichi Yamada 2, Ken-ichi Oinuma 3, Kanako Sato 4, Mamiko Niki 3, Hiroki Namikawa
More informationHeteroresistance to Meropenem in Carbapenem-Susceptible Acinetobacter baumannii
JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2009, p. 4055 4059 Vol. 47, No. 12 0095-1137/09/$12.00 doi:10.1128/jcm.00959-09 Copyright 2009, American Society for Microbiology. All Rights Reserved. Heteroresistance
More informationUCSF guideline for management of suspected hospital-acquired or ventilatoracquired pneumonia in adult patients
Background/methods: UCSF guideline for management of suspected hospital-acquired or ventilatoracquired pneumonia in adult patients This guideline establishes evidence-based consensus standards for management
More information