Proceedings of the 27th World Buiatrics Congress

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1 Close this window to return to IVIS Proceedings of the 27th World Buiatrics Congress June 3-8, 2012 Lisbon, Portugal Next Meeting : Jul. 27 Aug. 1, Cairns, Australia Reprinted in the IVIS website with the permission of the WBC

2 Posters Nutrition and metabolic diseases P: 300 Comparative study of metabolic mineral profiles in dairy farms in two regions of nariño, colombia Alejandro Cedeño, D.; Ceballos Marquez A.; Bolaños Alicia C.; Benevides B. University of Nariño, Colombia Objectives: The study aims to assess the concentration of Calcium, Phosphurus and Magnesium in the dry period, postpartum and peak production in dairy cows in two regions in Nariño, Colombia, located between 2600 and 3200 meters above sea level. Materials and Methods: The production system of dairy herds is classified as specialized in grazing with supplementation. In total 148 cows were evaluated of Pasto and 203 cows from Guachucal-Túquerres. The animals of each farm were divided into three groups. dry period, early lactation and peak production. Blood samples were taken in the evening, after milking. The statistical parameters included tests for normality of distribution, mean values, standard deviation, range and confidence interval at 95%. It set a significance level of p <0.05. Comparisons between groups were performed using analysis of variance, identifying sources of variation. Comparison of means (Tukey) was used in cases where the ANOVA was significant (p <0.05). Statistical analysis was performed with a Stata v (Stata Corp. College Satation, Tx, USA). Results: Calcium concentration showed variations regarding the production and calving time. In Pasto, cows showed variations between the peripartum period (p <0.05), while cows from Guachucal Tuquerres region no variations were found. There were no groups of cows with phosphorus values below 1.1 mmol / L. However, in farms in the region of Guacucal - Tuquerres groups of cows presented an average phosphatemia of 2.6 mmol / L, The magnesium concentration was not significantly different, according to the production stage (p> 0.05). In general the average values of Ca in cows of Pasto were lower in the three stages. A higher concentration of Ca in cows were found in Guachucal-Túquerres (P <0.05), according to the productive stage and calving. No differences were found for P and Mg concentration by region, productive stage or calving of the cow (P> 0.05). However, some P values above the upper reference limit (2.3 mmol / L) were presented. Conclusions: Cows in the dry season are considered in risk as animals in early lactation. The regions of Pasto and Guachucal-Túquerres have on average, different values for the metabolites in dairy cows similar to those observed in other regions of Colombia. The observed values, both high and decreased are an indication that there is presence of alterations that are a risk to the presentation of various kinds of diseases. The concentrations of phosphorus and magnesium were within the reference range P: 301 Comparative biochemical studies on adapted Holstein and pure Holstein dairy cows during transition period El Sebaie Hussien, A.; Abd-el-salam Naguib M.; Hussein Awad H.; Staufenbiel R. Faculty of Veterimnary Medicine- Assiut University, Egypt Objectives: The present study was designed for comparing some biochemical parameters reflecting the metabolic health status in adapted Holstein dairy cows and pure ones during their transition period. Materials and Methods: This study divided into two separate parts, the 1st part was carried out on 83 adapted Holstein cows in Egypt (Herd A) from 30 days prepartum to 30 days postpartum. The 2nd part of this study was carried out on a total 253 pure Holstein cows in Germany (Herd B) as 118 cows at 3 weeks prepartum and 135 cows at 3 weeks postpartum. In Herd A, after calving some cows suffered from clinical mastitis, while in Herd B some cows exhibited subclinical mastitis, subclinical ketosis, or combined subclinical ketosis and subclinical mastitis. Clinical examination was carried out on all animals included in this study as well as blood samples were collected from the tail vein for separation of serum samples that used for estimation of some biochemical parameters reflecting the metabolic health status including glucose, insulin, BHBA, NEFA, total bilirubin, total proteins, albumin, AST, GGT, calcium, phosphorous, and magnesium. Commercial test kits were used for estimation of the previously mentioned parameters. Results: In herd A, at 30 days postpartum, serum level of glucose was lower in mastitic adapted Holstein cows (1.4 mmol/l) than healthy ones (2.2 mmol/l), furthermore, albumin and calcium were decreased from 34.2±0.5 g/l and 2.3±0.05 mmol/l in healthy cows to 30.6±0.4 g/l and 2.1±0.04 mmol/l in mastitic ones, respectively, while globulins were increased from 23.4±1.1 g/l in healthy adapted Holstein to 37.4±4.6 g/l in mastitic ones. In herd B, at 3 weeks prepartum, serum level of BHBA was higher in subclinically ketotic mastitic pure Holstein cows (653 ± 50.4 µmol/l) than healthy ones (557 ± 25.1 µmol/l), while serum level of calcium was lower in subclinically ketotic (2.4 ± 0.02 mmol/l), subclinically mastitic (2.4 ± 0.03 mmol/l), subclinically ketotic mastitic cows (2.4 ± 0.2 mmol/l) than healthy ones (2.5 ± 0.03 mmol/l). In herd B, at 3 weeks postpartum, serum level of insulin decreased from 18.4 ± 3.26 pmol/l in healthy pure Holstein cows to 8.9 ± 0.84 pmol/l in subclinically ketotic ones, in addition, serum levels of BHBA and NEFA were higher in subclinically ketotic (1493 ± µmol/l and 772 ± 66.8 µmol/l) and subclinically ketotic mastitic (1725 ± µmol/l and 871 ± 84.8 µmol/l) groups than healthy ones (849 ± 38.2 µmol/l and 578 ± 62.2 µmol/l), respectively, while total bilirubin significantly increased in subclinically ketotic mastitic cows only (4.9 ± 0.51 µmol/l), furthermore, serum levels of GLDH and AST were higher in subclinically ketotic (35 ± 6.9 U/l and 122 ± 8.7 U/l) and subclinically ketotic mastitic (34 ± 6.5 U/l and143 ± 9.4 U/l) groups than healthy ones (29 ± 5.3 U/l and 97 ± 3.5 U/l), respectively. Conclusions: It is very interesting to say that the adapted Holstein dairy cows in Herd A did not subject to extreme metabolic health hazards during transition period as such in pure Holstein dairy cows in Herd B. P: 302 Periparturient disorders and its relation to subclinical ketosis in Iranian Holstein cows Sakha, M.; Nejat Dehkordi S. Science and research branch, islamic azad university, Islamic Republic of Iran Objectives: The objectives of this study were (i) to determine the incidence of ketosis in dairy herds under study using serum ß-hydroxybutyrate (BHB) concentrations, and (ii) to investigate its relationship with periparturient disorders and its economic loss. Materials and Methods: A total of 203 multiparous Holstein cow (parity 2-9) were randomly selected from 10 commercial dairy herds. Since the likely risk time for occurrence of ketosis is during 2-5 weeks after parturition, the samples were taken in 1 wk before calving and at wk 2 and 3 after parturition. From 3 weeks before calving to 2 months after calving, ß-hydroxybutyrate and glucose were measured by commercial kits(ranbut. Randox,UK and Parsazmun,Iran respectively) using automated analyser. peri partum disease information including milk fever, metritis, mastitis, clinical ketosis, displaced abomasum, retained placenta, pneumonia and lameness was captured in farm data sheet. Statistical analysiswere measured by descriptive analysis, Analysis of variance for significancy and chi-square test for relationship of diseases. Results: Statistical analysis revealed the prevalence of percent for subclinical ketosis(cut off point 1.2 mmol/l). There was a significant correlation between SCK and LDA(odds ratio 9.74) metritis (odds ratio and mastitis (odds ratio 6.64). Economic loss for SCK alone was about 41,4$ per case and with related metritis, mastitis and LDA, were 320 $, 150$ and 190$, respectively.there was significant correlation between subclinical ketosis and LDA, Metritis and Mastitis. Conclusions: Limited informations are available regarding the prevalence of subclinical ketosis in dairy herds and related diseases in Iran. There is considerable rate of subclinical ketosis and significant correlation with periparturient disorders in this study suggests an important alarm and more investigations for economic losses go to improve the nutritional management systems is neccassary. Actual economic loss would be more than estimation XXVII World Buiatrics Congress

3 Nutrition and METABOLIC DISEASES because loss of milk and weight loss due to related diseases and also medical monetary losses could be added.. P: 303 Correlation between a field glucose tolerance test (fgtt) in the transition dairy cow and b-ohb, glycemia and disease incidence detected in a field trial Riuzzi, S.; Fiore E.; Gianesella M.; Morgante M. Department of Veterinary Clinical Sciences, University of Padua, Italy Objectives: The objective of this study was to evaluate potential differences in cows response to a field glucose tolerance test (FGTT), performed during the transition period, and the possible relationship between the FGTT and the incidence of postpartum principal diseases. Materials and Methods: 100 dairy cows from 3 commercial dairies located in northern Italy, between 14 and 1 days before actual calving date (WEEK -1), have been randomly enrolled for participation in an observational study on FGTT. The same cows have been followed after calving for health check by veterinary diagnosis and submitted to an identical glucose challenge between 3 and 9 days postpartum (WEEK 1 PP). Furthermore, a blood sample was collected between 10 and 16 days postpartum (WEEK 2 PP). The GTT consisted in i.v. injection of 300 ml of 50% glucose solution (0,25g of glucose per kg of body weight). Glycemia was measured prior to treatment (T0), at 10 minutes (T10) and 80 minutes (T80) after the infusion and T80/T0 ratio was calculated in order to define the cow response to GTT. Each sample was analyzed on-farm using the Optium Xceed meter for determination of Glucose and B-OHB levels. B-OHB was measured twice post calving, both at T0 in WEEK 1 PP and WEEK 2 PP. Pearson correlations were calculated. Results: The results show a statistical significant positive correlation between the T80/T0 ratio (P<0.05, R2=0.318) and B-OHB within the WEEK 1 PP, a negative correlation between glucose at T0 postpartum and B-OHB (P<0.05, R2= in WEEK 1 PP; R2= in WEEK 2 PP) and a positive correlation between glucose at T80, independently of the WEEK sample, and metritis incidence (P<0.05, R2=0.221). No correlation was found with retained placenta diagnosis. Moreover, glucose at T0 has a positive correlation with glucose at T10 and T80 (P<0.05, R2=0,346 with T10; R2=0,584 with T80) and a negative relationship with T80/T0 ratio (P<0.05, R2=-0,422) independently of the WEEK sample, and this is also confirmed within each WEEK period. Similarly, glucose at T10 is positively correlated to T80 (P<0.05, R2=-0,584) at every WEEK period. Conclusions: In conclusion, a significant relationship was found between the circulating glucose levels after glucose administration and the B-OHB and basal glucose in the blood, detected by glucometers in field. Also, there is a relationship between the response to glucose load and the detection of some postpartum frequent health disorders as metritis and ketosis, that requires further investigation. P: 304 Suitability of mixing Catosal with commercially available saline Raposo Canelas, J.; Heep I.; Raposo Canelas J.; Taterra H. Bayer Animal Health, Germany Objectives: Catosal (synonyms Coforta and Phosphorum B12 ) is available as a ready-to-use solution for parenteral injection. It has been widely used as a roborant and metabolic stimulant in veterinary medicine since 1958 and currently is registered in 18 countries of the European Community and 47 further countries1. In cattle practice, Catosal is used, IV, SC or IM (depending from the label in each country, with a dosage from 10-25ml per animal, once or more) as supportive therapy for the treatment and prevention of a range of production-related disorders including ketosis, parturient paresis and other clinical conditions present during the transition period when metabolic imbalances are most likely to occur. As Vitamin B12 is known to degrade when exposed to oxygen and light2, and having this group received questions about the stability of Vitamin B12 when combined with commercial saline solutions exposed to both of the elements, the aim of the present investigation was to assess the stability of Vitamin B12 (cyanocobalamin) within a combination of Catosal and saline over time. Materials and Methods: To study stability, 20 ml Catosal were injected into 500 ml saline solution (NaCl, Isotonische Kochsalzlösung 0,9 %, Braun). One combination of Catosal and saline was protected with an aluminium foil, the other was not. Samples were taken at begin ( start ), after 2 hours, 4, 6 and after 12 hours of the combination. To determine the content of Vitamin B12 (cyanocoablamin), samples were analyzed immediately by HPLC. For calculation an internal standard was used. Results: Vitamin B12 (cyanocoablamin) is stable within saline solution, both covered and not covered, over the study timeline of 12 hours. Details are given in the table and table 1. After 12 hours, the concentration of Vitamin B12 (cyanocoablamin) slightly decreases in the saline solution without light protection (91 %) and it does not decrease in the light protected saline solution (100 %). These results are additionally shown in graph 1. Conclusions: It is concluded that Catosal mixed with commercially available saline solutions at the recommended dosage of 20 ml Catosal per 500 ml saline maintains it s Vitamin B12 content for 12 hours as shown above. To maintain the highest amount of Vitamin B12, it is recommended to protect the mixture from light using for example an aluminium foil. Further studies are necessary to access the interaction between Catosal and other commercial solutions than saline, as well it s clinical effect. P: 305 Changes in blood levels of glucose, insulin and inorganic phosphorus in healthy and ketotic dairy cows during glucose tolerance test Djokovic Dusan, R.; Samac H.; Ilic A Z.; Kurcubic S V.; Fratric P N. Faculty of Agronomy, Serbia Objectives: The objective of the present study was to evaluate blood glucose utilization by peripheral tissues based on changes in blood glucose, insulin and inorganic phosphorus levels in healthy and ketotic cows after intravenous infusion of glucose solution. Materials and Methods: Puerperal healthy (n=10) and ketotic (n=10) Holstein cows were injected intravenously via the jugular vein with a total of 500 ml of 50 % glucose solution. Blood samples were taken from the opposite jugular vein before and 30, 60, 120, 180 and 240 minutes after injection. Blood glucose and inorganic phosphorus levels and serum insulin levels were determined by the photometric and RIA methods, respectively. Results: After intravenous infusion of 500 ml of 50 % glucose solution, glucose and insulin blood values in both groups of cows increased significantly within 30 and 60 minutes of the experiment (P<0.05). After intravenous infusion of glucose, blood inorganic phosphorus levels decreased in both groups of cows. Within 180 and 240 minutes of testing there was a significant decrease (P<0.05) in blood inorganic phosphorus levels in ketotic cows as compared to healthy cows. The decrease in the blood level of inorganic phosphorus after glucose infusion is associated with active entry of glucose into the glycolytic pathway of peripheral tissues. Conclusions: The decrease in the blood level of inorganic phosphorus in both groups of cows during the experiment, the significant decrease in blood inorganic phosphorus levels in ketotic cows as compared to healthy cows, and the increase in blood insulin and glucose levels in both groups of cows suggest that glucose infusion has a therapeutic effect due to a higher degree of blood glucose utilization by peripheral tissues in ketotic cows. P: 306 Liver copper, selenium, and zinc profiles in relation to different production phases of a dairy herd Hussein Awad, H.; Müller A.; Staufenbiel R. Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Egypt Objectives: The present study was designed for estimating the liver profile of copper, selenium, and zinc at different stages of production cycle of a 96 Abstract Book

4 Nutrition and METABOLIC DISEASES dairy herd. Materials and Methods: A total number of 40 Holstein dairy cows were included in this study. They were classified according to their lactation stages into four different groups as dry-period stage (4-3 weeks antepartum), fresh-lactation stage (0-1 week postpartum), mid-lactation stage ( days postpartum), and late-lactation stage ( days postpartum). Each group (stage) consisted of 10 cows. All the animals originated a dairy herd of Brandenburg State. Liver samples were obtained from each cow as liver biopsies. Liver samples were frozen and Cu, Se, and Zn concentrations were later determined by inductivity coupled plasma emission spectroscopy. Results: Serum profiles of copper and zinc exhibited insignificant changes among different groups represented the different production phases (P > 0.05), furthermore, serum profile of selenium showed significant drop in cows of dry-period stage in comparison with fresh-lactation and mid-lactation stages (P < 0.05). Liver profile of copper was significantly lower in dryperiod and fresh-lactation stages in comparison with other production stages (P < 0.05); furthermore, liver concentration of selenium was significantly lower in fresh-lactation stage than other stages (P < 0.05), while liver zinc profile was insignificantly changed among different groups. Conclusions: Liver profiles of copper and selenium were under a physiological change during different production phases, therefore caution should be taken during evaluation their values. P: 307 Potential markers during transition period for early identification of subclinical ketosis and subclinical mastitis in Holstein dairy cows Hussein Awad, H.; El-sebaie A.; Abd-el-salam M.; Staufenbiel R. Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Egypt Objectives: The present study was designed for determining the role of prepartum body condition score (BCS) and ultrasound measurements of backfat thickness (BFT) and periparturient levels of beta hydroxybutyrate (BHBA) and non esterified fatty acids (NEFA) for early detection of subclinical ketosis and subclinical mastitis in Holstein dairy cows. Materials and Methods: This study was carried out on a total 253 multiparous Holstein cows as 118 cows at 3 weeks prepartum and 135 cows at 3 weeks postpartum. Cows were followed up during their first 2 months postpartum and according to their health status they were classified as clinically healthy group (n = 62) with fat to protein ratio of < 1.and somatic cell count < /ml, subclinically ketotic group (n = 72) with fat to protein ratio of = 1.5, subclinically mastitic group (n = 60) with somatic cell count of = /ml, and subclinically ketotic mastitic group (n = 59) with fat to protein ratio of = 1.5 somatic cell count of = /ml. The BCS for each cow, at each time was evaluated using a scale of 1-5 with 0.25 increments. On the same day, ultrasound measurements of backfat thickness were obtained using a portable Beta-mode ultrasound generator with a linear transducer and a frequency of 5 MHz. serum levels of BHBA and NEFA were estimated spectrophotometry using commercial test kits. Results: Body condition score and ultrasound measurements of BFT were the only significant predictors identified at 3 weeks prepartum as prepartum transit cows with a critical threshold of BCS or BFT of = 3.0 or = 28 mm and = 3.25 or = 22 mm were at great risk to develop subclinical ketosis and combined subclinical ketosis and subclinical mastitis, respectively during the first two months of subsequent lactation. At 3 weeks postpartum, BHBA was only identified as a predictor for subsequent disease as transit cows with BHBA =952µmol/l or =1220µmol/l were 2.9 or 2.4 times to develop subclinical ketosis or combined subclinical ketosis and subclinical mastitis during the first two months of subsequent lactation. Conclusions: Prepartum BCS or BFT and postpartum BHBA have potential as indicators and predictors for the disease in dairy cows. P: 308 Effect of supplementation with combination of vitamin A, E, C and volatile oils (Superimmune ) on growth rate and antioxidant parameters in calves. Youssef Ahmed, M.; Elseidy A Y.; El-khodery A S.; Abdelhamid M F.; Elgabry A M. Faculty of Veterinary medicine, Mansoura University, Egypt Objectives: The aim of the present study was to evaluate the Effect of supplementation with combination of vitamin E, A and C and volatile oils (Superimmune ) on growth performance and antioxidant parameters in calves. Materials and Methods: Twenty five Holstein calves weighing kg body weight at two weeks of age were used. Calves were randomly allocated into five groups (five each). The first group was supplemented daily with 10 ml of Superimmune (100,000 iu vit. A, 50mg vit E and 10mg vit.c, Eucalyptus oil, Lavender oil and Peppermint oil). the second group was supplemented with 100,000 vit A; the third group with 50mg vit. E and the fourth group with 10mg vit. C. The fifth group was kept without any treatment. Body weight, performance and Plasma analysis for oxidative stress markers and antioxidant enzymes were recorded pre-treatment were measured pre-treatment and weekly for 6 weeks post-treatment. Statistical analysis was performed using repeated measures MANOVA considering p value for MANOVA fit and Wilks Lambda test on both treatment and time. Results: Calves received a combination of vitamins and volatile oils showed significant increase of the body weight compared with control group at 6 weeks post-treatment; however, non-significant increase was recorded compared with supplementation of single nutrient. There was also a significant increase of Total antioxidant capacity (MANOVA fit:p<0.05, Wilks Lambda, p< 0.01), Glutathione reductase (GR) (MANOVA fit: p<0.05, Wilks Lambda p<0.05) and Glutathione-S-transferase (GST) (MANOVA fit:p<0.05, Wilks Lambda: p<0.05) in calves received combination compared with other groups. However, there was a significant decrease of malondialdehyde (MDA) (MANOVA fit: p<0.01, Wilks Lambda p<0.01). On the other hand, other parameters showed non-significant changes. Conclusions: The results of the present study indicate that a combination of vitamins and volatile oils may have better effect on body weight gain and antioxidant status in suckling calves than supplementation with single vitamin. P: 309 Usefulness of some biochemical parameters and electrocardiography technique in detection of nutritional muscular dystrophy of calves Sobiech, P.; Pomianowski A.; Zarczynska K.; Radwinska J. Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia-Masury, Poland Objectives: The objective of this study was to estimation of changes in some biochemical parameters during nutritional muscular dystrophy of calves and in addition was to detection of usefulness electrocardiography technique in early diagnosis of this disease. Materials and Methods: The study was performed on 20 H-F calves divided into two groups: I experimental group of 10 calves with symptoms of nutritional muscular dystrophy, II control group of 10 clinically healthy calves administered a single, preventive IM injection of vitamin E + Se preparation (Eurowet Animal Health BV) comprising tocopherol acetate 50 mg/ ml and sodium selenite 0.5 mg/ml at a dose of 2 ml/10 kg BW, on the second day after birth. The animals from both groups were subjected to clinical examinations, and blood samples were collected from the jugular vein at seven-day intervals, starting on day 5 (on day 5, 12 and 19). Electrocardiographic assessment was performed on day 19 in all calves. Blood serum samples were subjected to biochemical analyses to determine selenium and vitamin E concentrations, activity levels of aspartate aminotransferase (AST), creatine kinase (CK) and lactate dehydrogenase (LDH). Electrocardiography (ECG) examinations were performed on standing animals in the shed, using the BTL-08 MT electrocardiograph. To insulate patients from the ground, rubber mats with a thickness of 2 cm were placed under the calves. XXVII World Buiatrics Congress

5 Nutrition and METABOLIC DISEASES Results: Disease symptoms were observed in experimental group calves (not administered selenium and vitamin E supplements) in the first days of life. Signs of nutritional muscular dystrophy were reported in all experimental animals between day 10 and 17. The initial symptoms were manifested by apathy, lethargic behavior, decreased appetite and reluctance to move. They were followed by tremor in hind limbs, incorrect posture with widely spread limbs, hunched up spine and stilted gait In the group of calves with symptoms of nutritional muscular dystrophy, serum Se and vitamin E levels were considerably lower than in control (18.8 µg/l and 2.03 µg/ml respectively), the same activity of GSH-Px ( U/gHb) and these changes were accompanied by an increase in the activity of AST (92.3 U/l), CK (437.0 U/l) and LDH ( U/l). The electrocardiograms of experimental animals revealed elevated heart rate, normal and accelerated sinus rhythm, increased P wave amplitude, shorter PR interval, shorter QT interval, shorter ST interval, narrower QRS complex, shorter T wave duration and insignificantly increased T wave amplitude. Conclusions: The results of this study indicate that nutritional muscular dystrophy in calves is characterized by a drop in serum selenium and vitamin E concentrations, decreased levels of glutathione peroxidase activity and increased activity of aspartate aminotransferase, creatine kinase and lactate dehydrogenase. The disease is accompanied by cardiac dysrhythmia of various intensity, therefore, ECG examinations should be included in the protocol for routine diagnosis of NMD in calves. P: 310 Metabolic serum profiles in pre- and postpartum Rubia Gallega cattle Barrio, M.; Becerra J.; Quintela L.; Caínzos J.; Peña A.; Rodríguez-zamora A.; Trillo Y.; Prieto A.; Díaz C.; Peñarando D.; Herradón P. Reproducción y Obstetricia, Spain Objectives: The aim of this study was to establish, in the future, reference values for serum biochemical parameters in Rubia Gallega cattle breed. Materials and Methods: In 49 females, blood samples were taken 1 mo prior to calving and 60 d postpartum. Serum concentrations of total cholesterol, triglycerides, non-esterified fatty acids (NEFA), glucose, aspartate transaminase, alanine transaminase, total protein, albumin, urea, calcium, phosphorus, magnesium, were determined for each sample. Results: Mean values for metabolites analyzed were within the ranges described in the literature for cattle. Obtained results in prepartum and 60 days postpartum cows was as follows: Glucose (54.20 & 55,11 mg/dl), Cholesterol ( & 191,88 mg/dl), Triglycerides (16.19 & 15.43), Albumin (30.85 & 32,13 mg/dl), ALT (16.22 & U/l), AST (67.81 & U/l), Total Protein (5.75 & 6.36 g/l), NEFA (0.45 & 0.17 mmol/l), Urea (27.19 & mg/dl), respectively. Conclusions: Showed data representing mean values for a native breed, widely distributed in Galicia. P: 311 Lipidemia and lipid infiltration of the liver in dairy cows during the transition period Djokovic, R.; Samanc H.; Ilic Z.; Kurcubic V.; Fratric N. Faculty of Agronomy, Serbia Objectives: The objective of this experiment was to evaluate the correlation between blood lipid levels and liver lipid content in dairy cows during the transition period. Materials and Methods: Holstein dairy cows (n=40) were allocated to four groups: Group 1 included late pregnant healthy cows (n=10) from day 15 to day 5 before calving; Group 2 comprised late pregnant healthy cows (n=10) from day 5 to day 1 before calving; Group 3 included clinically healthy puerperal cows (n=10), and Group 4 comprised ketotic puerperal cows (n=10). Blood concentrations of beta-hydroxybutyrate, glucose, free fatty acids, triacylglycerols, and total cholesterol were determined following the photometric methods. The liver tissues obtained by percutaneous biopsy and the liver sections were stained with Sudan III for lipids. Hepatocyte lipid content was determined by the Leica Q 500 MC image analysis system (Software Q Win). Results: Pathohistological analysis of liver samples showed statistically signifiantly higher lipid infiltration in hepatocytes (P<0.01) in ketotic cows as compared to healthy cows during late pregnancy and puerperium. Biochemical analysis of blood showed significantlly higher (P<0.01) values of free fatty acids and beta-hydroxybutyrate and significantlly lower concentrations of glucose (P<0.01), triacylglycerols (P<0.01) and total cholesterol (P<0.05) in ketotic cows as comared to the values obtained in blood of healthy cows. A positive correlation (r=0.58, P<0.05) was observed between blood levels of free fatty acids and liver lipid content in ketotic cows. Conclusions: The significant increase in the blood concentrations of free fatty acids, the positive correlation between blood levels of free fatty acids and liver lipid content in ketotic cows, and the pathohistological report show that newly synthesized triacylglycerols accumulated in the hepatocytes during intensive lipomobilization. It was suggested that the significant increase in the blood levels of free fatty acids and the significant decrease in blood triacylglycerol and total cholesterol levels in ketotic cows may be major indicators of hepatic lipidosis. P: 312 Use of individual cow monthly milk fat records as a screening tool for the identification of subacute ruminal acidosis affected herds Valergakis E, G. Department of Animal Production, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece Objectives: The objective was to evaluate individual monthly milk fat records as indicative of subacute ruminal acidosis (SARA) in dairy herds and identify factors that affect them. Materials and Methods: Official monthly milk recording data from 18 farms ( Holsteins, 26.3±3.6 kg of milk/day, 3.8±0.2% fat) were used. Individual milk fat records more than 1% lower than the herd s bulk tank fat record (LIMFR) were considered suspicious for SARA. Analysis included: a) all 2598 cows and b) cows with less than 50% of their records as LIMFR and those with three or more records (2112 cows). When =10%, between 5% and 10% and >5% of the cows had LIMFR, these were considered SARA alert, SARA at-risk and SARA negative months, respectively and herds were classified in three groups based on their frequency. Differences in prevalence, incidence, % of cows with consistently low milk fat records (CLMFR) and different SARA months among herd groups and the effect of SARA alert and at-risk months on monthly bulk tank milk fat record were evaluated using ANOVA. A general linear model was used to evaluate the effect of herd size, milk production and herd fat record on the above parameters. Results: When all cows were included in the analysis, prevalence was 8.1±2.8% and incidence was 35.5±12.4%. CLMFR cows were 3.2±2.6%. SARA alert, at-risk and negative months were 5.1±3.6, 5.0±2.4 and 4.9±2.8, respectively. Prevalence, incidence and number of CLMFR cows among groups (best, average and worst herds) were different (P<0.001, P<0.05 and P<0.05, respectively). After edits, prevalence was 6.6±2.1% and incidence was 38.0±12.3%. SARA alert, at-risk and negative months were 3.2±2.2, 4.9±2.3 and 6.9±2.8, respectively. Prevalence and incidence among groups were again different (P<0.001 and P<0.01, respectively). Only 8 of the herds retained their original classification. Number of SARA alert and negative months was significantly different (P<0.001) among groups both before and after edits. Herd size had a significant negative effect on prevalence (P<0.05) both before and after edits. Herd size and herd fat record had a significant negative effect on incidence both before (P<0.01 and P<0.05, respectively) and after edits (P<0.001 and P<0.05, respectively). Conclusions: Based on the considerable variation among dairy herds, individual monthly milk fat records can be used as an initial screening test for SARA. At the same time, number of CLMFR cows and management ((larger herds usually feed TMRs) issues should be taken into account. 98 Abstract Book

6 Nutrition and METABOLIC DISEASES P: 313 Bovine subclinical ketosis in dairy herds in Nishaboor Iran Tehrani-sharif, M. Islamic Azad University- Garmsar Branch, Islamic Republic of Iran Objectives: Among domestic farm animals, the metabolic diseases achieve their greatest importance in dairy cows and pregnant ewes. As milk production in dairy cows increases and as herds become larger, the incidence of metabolic disease increases.minimizing the occurrence, severity, and consequences of negative energy balance in the early postpartum period has become an important issue for the dairy industry. One of the consequences of negative energy balance is subclinical or clinical ketosis.the aim of this study was to determine the prevalence of subclinical ketosis (SCK) in postpartum cows in Nishaboor Iran. Materials and Methods: One hundred ninety lactating cows in 2-4 wks of lactation From fifteen herds in Nishaboor were included in this study. Blood samples were collected via coccygeal vein & transported on ice to laboratory and serum separation was done in 60 min. Serum samples were kept at -20 till the laboratory analysis. the concentration of ß- Hydroxy Butyrate (BHB), Activity of AST & glucose were determined.the distribution of ß- Hydroxy Butyrate (BHB) results was skewed around 1400 µmol/l, so it defined as the cut off point. Results: % (29/190) of dairy cows were positive if 1400 µmol/l assumed as the cut off point. If 1200 µmol/l considered as the cut-off point, 18.42%(35/190) were positive. The mean ± SD of glucose & BHB in ketotic and non ketotic cows were ± 3.95 mg/dl, 43.94±0.8 mg/dl & 2.7±0.26 mmol/l, 0.64±0.22 mmol/l respectively. Blood Glucose concentration in subclinical ketotic cows were significantly lower (p = 0.05) than in non ketotic cows. The concentrations of BHB and glucose in serum were significantly (p = 0.01) and inversely correlated (r = ; P = 0.05) in the tested cows. Postpartum disorders (Metritis, LDA, retained placenta, mastitis, Dystocia, lameness) were seen in 62.1 % (18/29) of ketotic cows. Conclusions: The results of this study showed that the prevalence of subclinical ketosis is higher than other countries & according to these results, regular screening tests are recommended. P: 314 Bovine subclinical ketosis in dairy herds in Nishaboor Iran Tehrani-sharif, M. Islamic Azad University- Garmsar Branch, Islamic Republic of Iran Objectives: Among domestic farm animals, the metabolic diseases achieve their greatest importance in dairy cows and pregnant ewes. As milk production in dairy cows increases and as herds become larger, the incidence of metabolic disease increases.minimizing the occurrence, severity, and consequences of negative energy balance in the early postpartum period has become an important issue for the dairy industry. One of the consequences of negative energy balance is subclinical or clinical ketosis.the aim of this study was to determine the prevalence of subclinical ketosis (SCK) in postpartum cows in Nishaboor Iran. Materials and Methods: One hundred ninety lactating cows in 2-4 wks of lactation From fifteen herds in Nishaboor were included in this study. Blood samples were collected via coccygeal vein & transported on ice to laboratory and serum separation was done in 60 min. Serum samples were kept at -20 till the laboratory analysis. the concentration of ß- Hydroxy Butyrate (BHB), Activity of AST & glucose were determined.the distribution of ß- Hydroxy Butyrate (BHB) results was skewed around 1400 µmol/l, so it defined as the cut off point. Results: % (29/190) of dairy cows were positive if 1400 µmol/l assumed as the cut off point. If 1200 µmol/l considered as the cut-off point, 18.42%(35/190) were positive. The mean ± SD of glucose & BHB in ketotic and non ketotic cows were ± 3.95 mg/dl, 43.94±0.8 mg/dl & 2.7±0.26 mmol/l, 0.64±0.22 mmol/l respectively. Blood Glucose concentration in subclinical ketotic cows were significantly lower (p = 0.05) than in non ketotic cows. The concentrations of BHB and glucose in serum were significantly (p = 0.01) and inversely correlated (r = ; P = 0.05) in the tested cows. Postpartum disorders (Metritis, LDA, retained placenta, mastitis, Dystocia, lameness) were seen in 62.1 % (18/29) of ketotic cows. Conclusions: The results of this study showed that the prevalence of subclinical ketosis is higher than other countries & according to these results, regular screening tests are recommended. P: 315 Relationship between the oxidative status and the protein metabolism of dairy cows during the transition period Abuelo Sebio, A.; Hernández Bermúdez J.; Pereira Lestayo V.; Benedito Castellote Luis J.; García Vaquero Antonio M.; Castillo Rodríguez C. Department of Animal Pathology - University of Santiago de Compostela, Spain Objectives: This study was designed to evaluate, under field conditions, the relationship between the oxidative stress biomarkers and the protein metabolism of dairy cows in the transition period. Materials and Methods: 57 HF multiparous cows were used. The control group was composed by 40 animals in the 4th-5th month of gestation, when neither lactation nor pregnancy were major metabolic burdens. The experimental group consisted in 17 pregnant animals. Each experimental cow was sampled twice in each of the following stages: 1) late lactation (-2 to -1 month); 2) prepartum (-1 month until parturition); 3) postpartum (delivery to +1 month) and 4) peak of lactation (+1 month to +2.5 months after parturition). Reactive Oxygen Metabolites (ROM), Plasma Barrier to Oxidation (OXY), serum total proteins (STP), albumin, serum urea nitrogen (SUN) and creatinine were measured in serum samples spectrophotometrically. The Oxidative Stress index (OSi) was calculated as ROM/OXY. Correlations between parameters were established using the Pearson correlation coefficient at a P<0.05. Results: STP levels only showed a negative correlation (r=-0.461) with ROMs within the prepartum stage. Albumin was positively correlated with OXY in the control animals (r=0.386) and a negative correlation with OSi (r=-0.599) was found; confirming its well-known antioxidant role. SUN was negatively correlated with ROMs in the control group (r=-0.333) and in the prepartum stage (r=-0.675) as well as with OSi in prepartum (r=-0.615) and postpartum stages (r=-0.572); it showed also positive correlation with OXY in the control group (r=0.352) and in the postpartum stage (r=0.539), suggesting that this metabolite acts as an endogenous antioxidant, neutralising the production of oxidants and reducing the risk of oxidative stress. Creatinine levels showed, in control animals, a positive correlation with OXY (r=0.359) and a negative one with the OSi (r=-0.341), which seems to indicate that this metabolite could also be implicated in the serum antioxidant capacity. This finding was not, in the best of our knowledge, previously reported in cattle. Conclusions: We conclude that the usual drop in the levels of albumin, urea and creatinine after parturition implies a reduction in the antioxidant defense of the animals, which can expose them to a greater risk of oxidative stress. Therefore, further studies are needed to establish the antioxidant role of creatinine in cattle, as well as for urea, due to its particular metabolism in ruminants; as these parameters can be influenced by diet. P: 316 Relationship between different energetic status biomarkers during the transition period in high yielding dairy cows. Hernández Bermúdez, J.; Benedito Castellote Luis J.; Abuelo Sebio Á.; Pereira Lestayo V.; Miranda Castañón Inés M.; García Vaquero Antonio M.; Castillo Rodríguez C. Department of Animal Pathology - University of Santiago de Compostela, Spain Objectives: The aim of the present study was to evaluate changes on selected energetic metabolic parameters during transition period, as well as relationships between them. Materials and Methods: The study was carried out using 17 healthy multiparous pregnant Holstein cows maintained in a commercial dairy herd. All XXVII World Buiatrics Congress

7 Nutrition and METABOLIC DISEASES cows had a normal calving and no clinical disturbances were recorded during the postpartum period. The calving season was in winter. During the study period all animals were kept under identical conditions. Every experimental cow was sampled in: i) late lactation period (from -2 to 1 month prior parturition), ii) close- up period (-1 month until parturition); iii) early lactation (delivery to 1 month postpartum) and iv) peak of lactation (+1 month to +2.5 months after parturition). Metabolic parameters (serum glucose, NEFA, and beta-hydroxybutyrate (BHB) were measured in serum samples spectrophotometrically, whereas insulin was assayed using an ELISA specific commercial kit. BCS was also monitored. All statistical analyses were performed using the IBM SPSS v.19 software package. Results: Starting with serum glucose values, we found that glucose trend to decrease progressively throughout the study, with lowest values in lactation period. For serum NEFA and BHB, we found that, before partum, cows reached the highest values, decreasing after it, showing the lowest value at the same time that glucose. In relation to serum insulin, the lowest value appeared before parturition, increasing dramatically after parturition in the lactation peak. Before parturition cows showed the highest value of BCS, reaching the lowest one in early lactation. Considering the relationships between all the parameters in each phase, we found that only serum glucose presented statistical and negative correlations with others. Conclusions: We can conclude that serum glucose is connected with other parameters, since it plays a central role in the metabolism as the primary metabolic fuel. Notwithstanding, according to previous reports, glucose an insensitive marker of energy status in cattle due to its homeostatic regulation. P: 317 Relationship between the oxidative status and the energetic profile of dairy cows during the transition period Hernández Bermúdez, J.; Abuelo Sebio Á.; Benedito Castellote Luis J.; Pereira Lestayo V.; García Vaquero Antonio M.; Castillo Rodríguez C. Department of Animal Pathology - University of Santiago de Compostela, Spain Objectives: This study was designed to evaluate, under field conditions, the relationship between the oxidative stress biomarkers and the energetic metabolism of dairy cows in the transition period. Materials and Methods: 57 HF multiparous cows from the same herd were used. The control group was composed by 40 animals between the 4th and 5th month of gestation, when neither lactation nor pregnancy were major metabolic burdens. The experimental group consisted in 17 pregnant animals. In order to relate our results with the physiological status of the animals, the data of these experimental animals were divided ex-post into four stages: i) late lactation (-2 to -1 month); ii) prepartum (-1 month until parturition); iii) postpartum (delivery to +1 month) and iv) Peak of lactation (+1 month to +2.5 months after parturition). Reactive Oxygen Metabolites (ROM), Plasma Barrier to Oxidation (OXY), glucose, non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHB) were measured in serum samples spectrophotometrically. The Oxidative Stress index (OSi) was calculated as ROM/OXY. Correlations between parameters were established using the Pearson correlation coefficient. Results: No significant correlation was found between the NEFA values and the levels of ROM, OXY or OSi. The levels of BHB were only significant correlated in the postpartum stage with the OSi (r=0.539; P<0.05), albeit the negative correlation in this stage with OXY represented a tendency to a difference (r=-0.449; 0.05) Conclusions: These findings suggest us that the negative energy balance that usually the dairy cows suffer in the peripartum period, and the consequent adaptation of their metabolism, expose them to a higher risk of oxidative stress; which can increase the susceptibility of dairy cattle to a variety of health disorders, especially metabolic diseases after parturition. P: 318 Long-term measurement of rumen ph in fattening bulls by an indwelling and wireless data transmitting unit Gasteiner Bernhard, J.; Velik M.; Guggenberger T. Research Centre Gumpenstein, Austria Objectives: Subacute rumen acidosis (SARA) is a significant production disease of fattening bulls. The objective of this study was the continuous and long term measurement of the ruminal ph in fattening bulls under defined and exact feeding conditions. Materials and Methods: In the present study 40 bulls were fattened with a typical Austrian bull ration (corn silage ad libitum and concentrates). For measuring ruminal ph, an indwelling system for monitoring ruminal ph and temperature was applied. The indwelling system was given orally to fattening bulls at body weight (BW) 325 kg up to 625 kg stepwise, 16 Sensors in total, to cover the whole fattening period. Ruminal ph and temperature were measured at intervals of 600 sec over a period of 50 days of fattening period. Data were collected in an internal memory chip and sent via radio transmission to an external receiver. Daily mean, minima, maxima and time ruminal ph (min/d) below 6.3; 6.0; 5.8 and 5.5 were calculated. Individual daily dry matter intake, feeding conditions and ration composition in terms of roughage and concentrate sources were determined and nutrient components were analysed. Statistical analysis was conducted by GLM (Statgraphic Plus 5.1). Results: Radio transmission of data (twice daily) was functioning without any difficulties. Mean ruminal ph for all bulls over the whole fattening period was 6.54, decreasing from ph 6.74 (BW 325 kg) to ph 6.44 (BW 625 kg). Lowest mean ph was found in group BW 475 (ph 6,17), caused by an outbreak of Bovine Despiratory disease, which led to decreased roughage intake whilst intake of concentrates was not reduced. In general, rumen ph was correlated significantly with the body weight and with body weight gain due to the animals increasing dry matter intake. Rations starch content and total starch intake influenced ruminal ph significantly. The rations fibre content was above recommendations during the whole fattening period and hence no correlation between fibre supply and ruminal ph was found. Conclusions: Recent progression in wireless and microcomputing enable the use of intraruminal boluses, that can be inserted orally. Boluses used in this trial are collecting valid data for at least 50 days. Such continuous measurements provide a complete new insight in rumen physiology and pathology. Results in our trial were significantly influenced by the rations composition, by the dry matter intake and thus by the fattening period. Results show that the presented method is a very useful and proper tool for both, scientific and practical applications P: 319 Influence of grain processing (grinding or pelleting) on serum metabolites and enzymes in finishing bull calves Pereira, V.; Hernández J.; Benedito J.; Vázquez P.; Abuelo Á.; García-vaquero M.; Muiño R.; Castillo C. Department of Animal Pathology. University of Santiago de Compostela, Spain Objectives: The aim of this study was to compare the effect of two different methods of grain processing, grinding and steam pelleting, on different serum metabolic parameters (glucose, non-esterified fatty acids (NEFA), serum urea nitrogen (SUN), Total Serum Protein (TSP), albumin, creatinine, L-lactate, aspartate aminotransferase (AST),?-glutamyl transferase (GGT) and amylase in finishing bull calves. Materials and Methods: A 77-day feedlot metabolic study was conducted using twenty Belgian Blue bull calves. Animals were allotted randomly into each of two experimental groups: PF (n=10), fed steam-pelleted concentrate; and GF (n=10), fed ground concentrate. All metabolic parameters were measured spectrophotometrically in serum samples. All statistical analyses were performed using SPSS The criterion for statistical significance was P = 0.05; P values between 0.05 and 0.1 were considered trend to significant. 100 Abstract Book

8 Nutrition and METABOLIC DISEASES Results: Groups PF and GF did not differ significantly in regard to either glucose, NEFA levels, albumin, TSP, and serum enzymes AST, GGT and amylase, although these variables varied significantly throughout the time. SUN and serum creatinine showed statistical differences between group x time interaction, finding the highest values of both in PF group (unless sample corresponding to day 51 for SUN value). Only serum L lactate didn t show any change associated to grain processing, even though PF group showed numerically the highest values, in relation to GP. Conclusions: In our fields conditions, surprisingly only two parameters (SUN and creatinine values) showed a direct influence by grain processing, and taking into account that grain processing, at least from a theoretically point of view, increases the structural disruption of grain, it should increase the availability of glucose precursors and change protein solubility within the rumen, modifying many metabolites. The lack of effect may be derived to other nutritional factors, such as fibre source or crude protein of the ration. P: 320 Study of metabolic profile and productive performance in finishing bull-calves fed a high-grain diet supplemented with sodium-malate Pereira, V.; Hernández J.; Benedito J.; Vázquez P.; López-alonso M.; Abuelo Á.; Muiño R.; Castillo C. Department of Animal Pathology. University of Santiago de Compostela, Spain Objectives: This study investigated the effects of commercial malate salt supplementation on metabolic parameters and final productive performance in finishing-stage Belgian Blue bull calves maintained in a commercial feedlot, considering both short- and long-term effects on the parameters considered. Materials and Methods: An 86-day feedlot metabolic study was conducted using 26 Belgian Blue bull calves. Animals were allotted randomly to one of the two experimental groups: 1) control group [no supplementation; n=10, group C]; 2) supplementation with 2.8 g of disodium malate-calcium malate (Rumalato ) per kg (DM basis) [n=16, group MS]. The serum parameters studied were glucose, non-esterified fatty acids (NEFA) and L-lactate. All metabolic parameters were measured in serum samples spectrophotometrically. Productive parameters considered were average daily gain (ADG), daily intake (DI) and feed to gain ratio. All statistical analyses were performed using SPSS The criterion for statistical significance was P = 0.05; P values between 0.05 and 0.1 were considered trend to significant. Results: The malate-supplemented calves showed slightly lower mean ADG and mean feed intake than the control animals, but the difference in ADG was not statistically significant. Feed-to-gain ratio was similar in the two groups. Considering these results the malate supplementation appears to have no beneficial effects probably attributable to the diet characteristics. Even though we did not find statistical differences on NEFA or glucose levels, we could appreciate a numerical decrease on NEFA levels in MS group. Finally, despite the well-known in vitro effects of malate on lactate levels, in the present study serum L-lactate remained higher in malate-supplemented animals than in controls, suggesting that the effects of malate can be dependent on the characteristics of the diet being fed. Conclusions: The lack of effect of malate supplementation on glucose levels during all productive cycle fits with the absence of improve of the productive parameters. Nevertheless, the effect of malate supplementation on NEFA levels during the finishing period show an appropriate energetic balance. P: 321 In vitro and in vivo evaluation of an autolysed yeast preparation (Levabon Rumen) as feed supplement Ganner, A.; Stoiber C.; Dohnal I.; Markert W.; Ribeiro Gomide D.; Deckardt K.; Klevenhusen F.; Pereira Neves M.; Zebeli Q.; Schatzmayr G. BIOMIN Research Center, Austria Objectives: The beneficial effects of yeasts in dairy industry are known and utilized for more than one century. Target of the present study was to evaluate how supplementation of a novel yeast derivative (Levabon Rumen) affects rumen in vitro and in vivo rumen fermentation parameters as well as feed intake. Levabon Rumen represents a spray-dried yeast product (Saccharomyces cerevisiae) which is manufactured by an internally developed autolysis process, optimized to retain the biological value from a mixture of cell wall carbohydrates, peptides, essential amino acids and B-vitamins. Materials and Methods: In a first in vitro batch culture study, rumen simulation reactors were filled with rumen fluid, mixed with artificial saliva and incubated under anaerobic conditions. In a second long-term incubation experiment with the rumen simulation technique (RUSITEC), different levels of the yeast product were fed daily over a period of 14 days under a constant flow of artificial saliva. The samples were mixed into a ration consisting of 50% hay and 50% concentrate and sealed in nylon bags (pore size 150 µm). After a 7 day adaptation-phase samples were collected for 7 days. The parameters measured include nutrient digestibility, concentrations of volatile fatty acids, gas production, and total bacterial and protozoal counts. Subsequently, an in vivo trial was undertaken with nine rumen fistulated Holstein Heifers, allocated to a sequence of three treatments (control, 10g and 30g yeast/heifer/day), in 35-day periods, 3x3 Latin Squares. Yeast was given twice daily via fistula. Results: Preliminary results indicate improved digestibility in RUSITEC and increased amount of anaerobic bacteria in batch culture in vitro, and improved dry matter intake as well as improved digestibility in vivo. Conclusions: Based on these findings, the autolysed yeast product represents a promising feed additive to improve cattle health and performance. Further research is warranted to understand the underlying mechanisms of the positive effects of yeasts and determine their effects on rumen health. P: 322 Association among blood metabolites variations, metabolic diseases and reproductive disorders in transition dairy cows Badan, M.; Stefani Lisa A.; Bertazzo V.; Ruffa M.; Dorigo M.; Barberio A. Istituto Zooprofilattico Sperimentale delle Venezie, Italy Objectives: The aim of the study was to investigate the changes of some metabolic parameters in dairy cows during transition period and their relation with postpartum metabolic disorders and reproductive diseases. Materials and Methods: The study was carried out on 6 dairy farms in North-East of Italy, (herd size: cows). In all farms, cows were housed in free stalls and fed with total mixed ration (TMR) twice a day. Two blood samples were collected from 12 randomly selected cows in each farm, one at 10±5 days before calving estimated date, and the other at 10±5 days after calving. A different group of 12 cows was sampled during the year, at the beginning of each season (spring, summer, fall and winter). Blood samples were analyzed for major biochemical metabolites and IGF1 contents. All tested cows were controlled for reproductive diseases 3 times after calving, at 10, 30, 60 days, and any clinical signs of reproductive and metabolic disease were recorded. Statistical analysis was performed using chi-squared test to evaluate the association among biochemical metabolites and disease occurrence. Results: A significant association was found between NEFA and bilirubin increase, and the development of postpartum disorders. More specifically, a NEFA value >0.50 mmol/l was significantly associated (p<0.1) to metabolic diseases in postpartum period, and bilirubin value>8.00?mol/l was significantly associated with metabolic diseases and clinical mastitis (p<0.05), and with reproductive diseases (especially ovarian cysts and metritis) with a value of p < 0.1. Furthermore, 50 samples with normal concentrations of NEFA and bilirubin, and 59 samples with a content of NEFA and bilirubin respectively >0.50 mmol/l and >8.00 µmol/l were tested to compare IGF-1 content. Results showed that mean serum IGF-I concentration was significantly lower (p<0.05) in cows with high NEFA and bilirubin content. Conclusions: These data suggest that NEFA and bilirubin could represent important predictive parameters to assess the risk of metabolic disorders in post-partum. Bilirubin was found to be correlated also with post-partum reproductive disorders, but with a weaker statistical association. Results XXVII World Buiatrics Congress