CURRICULUM VITAE POSITION TITLE INSTITUTION AND LOCATION DEGREE YEAR FIELD OF STUDY

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1 CURRICULUM VITAE POSITION TITLE Pablo J. Pinedo EDUCATION Assistant Professor of Ruminant Animal Health Texas A&M AgriLife Research & Extension Center College of Veterinary Medicine & Biomedical Sciences Texas A&M University System, Amarillo TX INSTITUTION AND LOCATION DEGREE YEAR FIELD OF STUDY CVM University of Florida, Gainesville, FL PhD 2008 Veterinary Medicine University of Chile, Santiago, Chile D.V.M 1993 Veterinary Medicine Contact Information Phone: / e mail: ppinedo@ag.tamu.edu Professional Experience 2011 to present Assistant Professor of Ruminant Animal Health, Texas A&M AgriLife Research & Extension Center (60%) Department of Veterinary Pathobiology. College of Veterinary Medicine & Biomedical Sciences, Texas A&M University System (40%) Residency, Large Animal Clinical Sciences, University of Florida College of Veterinary Medicine (Food Animal Reproduction and Medicine Service) Postdoctoral position, Department of Animal Sciences, University of Florida. DHIA data analyses to explore the dynamics of culling in dairy systems and its association with diverse risk factors (Dr. Albert De Vries) PhD program, College of Veterinary Medicine. Cumulative UF GPA: 4.0. Program centered on Johne s disease diagnostic, epidemiology, and genetic factors associated to susceptibility to paratuberculosis infection (microsatellite and SNP analyses). Dissertation title: Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection (Dr. Owen Rae) Teacher Assistant in Dairy Production (UF, CVM) INSECABIO (Bovine genetics company, Chile). Dairy Recording System manager and cattle genetics specialist. Assistance to dairy farmers in breeding programs and use of information for management decisions. Management of cattle classification and mating programs Veterinary consultant in San Rafael cow calf operation (Chile) 1994 Veterinary consultant in Rinconada de Tinguiririca dairy farm (Chile) College of Veterinary Medicine (U. of Chile), Teacher Assistant in Animal Genetics Pablo J. Pinedo June 2014 Page 1

2 1993 Best student of the Veterinary School 1993 class, University of Chile 1992 College of Agricultural Sciences (Catholic University of Chile), Teacher Assistant in Animal Genetics Training and Technical Tours May 2012 Dec 2003 May 2003 Oct 2000 Sept 1997 May 1996 Dairy Genetic Showcase (Texas) Genetic tour to New Zealand. Topic: Genetic of composite (crossbreeding systems in bovine and ovine) Dairy tour to France. Topic: Performance of Montbeliarde dairy breed Dairy tour and World Dairy Expo (Madison, USA) Dairy tour to Canada (Montreal Quebec). Topic: Information systems for management and health in dairy herds Dairy tour to United States (Wisconsin California). Topic: Dairy systems and dairy genetics Research Interests/Areas of Expertise Host genetics and non traditional selection traits (Health, survival, and fertility) Data management in dairy systems Preventive dairy production medicine Paratuberculosis genetics, epidemiology, and diagnostics Selected Peer reviewed Publications and Book Chapters Khol J.L., Pinedo P.J., Buergelt C.D., Neumann L., Rae D.O., Lymphatic fluid for the detection of Mycobacterium avium subsp. paratuberculosis in cows by PCR, compared to fecal sampling and detection of antibodies in blood and milk. Veterinary Microbiology. 172: J.H.J. Bittar, P.J. Pinedo, C.A. Risco, J.E.P. Santos, W.W. Thatcher, K.E. Hencken, S. Croyle, M. Gobikrushanth, C.C. Barbosa, A. Vieira Neto, K.N. Galvão Inducing ovulation early postpartum influences uterine health and fertility in dairy cows. J. Dairy Science 97: P. J. Pinedo, A. Daniels, J. Shumaker, and A. De Vries Dynamics of culling for Jersey, Holstein, and Jersey x Holstein crossbred cows in large multibreed dairy herds. J. Dairy Sc. 97: Pinedo, P.J., Galvão, K.N., Seabury, C.M Innate immune gene variation and differential susceptibility to uterine diseases in Holstein cows. Theriogenology 80: Pinedo, P.J., Karreman, H., Bothe, H., Velez, J., Risco, C Efficacy of a botanical preparation for the intramammary treatment of clinical mastitis on an organic dairy farm. The Canadian Veterinary J. 54: Richeson, J.T., Pinedo, P.J., Kegley, E.B., Powell, J.G., Gadberry, M., Beck, P.A., Falkenberg S.M Association between hematological parameters and gender upon arrival with clinical Pablo J. Pinedo June 2014 Page 2

3 bovine respiratory disease risk in newly received beef calves. J Am Vet Med Assoc. 243: Buttrey, E.K., Jenkins, K.H., Lewis, J.B., Smith, S.B., Miller, R.K., Lawrence, T.E., McCollum III, F.T., Pinedo, P.J., Cole, N.A., and MacDonald, J.C Effects of 35% corn wet distillers grains plus solubles in steam flaked and dry rolled corn based finishing diets on animal performance, carcass characteristics, beef fatty acid profile, and sensory attributes. J. Anim. Sci. 91: Wang X., Gautam, R., Pinedo, P.J., Allen, L.J.S., Ivanek, R. J. A stochastic model for transmission, extinction and outbreak of Escherichia coli O157:H7 in cattle as affected by ambient temperature and cleaning practices. Mathematical Biology. In press. Gautam, R., Pinedo, P.J., Park, S., Ivanek, R Short communication: The distribution of drinking water to cattle ratios in the summer across four feedlots in the Texas High Plains. J. Agricultural Sciences 6: Khol J.L., Pinedo P.J., Buergelt C.D., Neumann L., Baumgartner W., Rae D.O., 2012.The collection of lymphatic fluid from the bovine udder and its use for the detection of Mycobacterium avium subsp. paratuberculosis in the cow. J. Vet. Diagn. Invest. 24: Pinedo, P.J., Fleming, C., Risco, C.A Events occurring during the previous lactation, the dry period, and the peripartum as risk factors for early lactation mastitis in cows receiving two different intramammary dry cow therapies. J. Dairy Sci. 95: Pinedo, P.J., Risco, C., Melendez, P., A retrospective study on the association between different dry period lengths and somatic cell counts, milk yield, reproductive performance, and culling in Chilean dairy cows. J. Dairy Sci. 94: Pinedo P.J., De Vries, A Dynamics of culling risk with disposal codes reported by DHI dairy herds. J. Dairy Sci. 93: Pinedo, P.J., Webb D., De Vries, A., Effect of days to conception in the previous lactation on the risk of death and live culling around calving. J. Dairy Sci. 93: De Vries, A., Olson, J.D., Pinedo, P.J., Reproductive risk factors for culling and productive life in large dairy herds in the eastern United States between 2001 and J. Dairy Sci. 93: Pinedo, P.J., Melendez, P., Temporal patterns of bulk tank somatic cell count, fat, protein, and urea nitrogen and their association with fertility in central southern Chilean dairy cattle. Arch Med Vet. 42, Pinedo, P.J., Melendez, P., Villagomez Cortes, J.A., Risco, C.A.., Effect of High Somatic Cell Counts on Reproductive Performance of Chilean Dairy Cattle. J. Dairy Sci. 92: Pinedo, P.J., Buergelt, C.D., Donovan, G.A., Melendez, P., Morel, L., Wu, R., Langaee, T.Y., Rae, D.O., Association between CARD15/NOD2 gene polymorphisms and paratuberculosis infection in cattle. Vet. Microbiol. 134, Pinedo, P.J., Rae, D.O., Williams, J.E., Donovan, G.A., Melendez, P., Buergelt, C.D., Association among serum ELISA, fecal culture, and nested PCR on milk, blood, and feces for the detection of paratuberculosis infection in dairy cows. Transboundary and Emerging Diseases. 55, Pablo J. Pinedo June 2014 Page 3

4 Pinedo, P.J., Buergelt, C.D., Monif, R.G., Williams, J.E., Rae, D.O., Mycobacterium avium subspecies paratuberculosis shedding into milk: Correlation of serum ELISA titers with DNA detection in milk. J. Appl. Res. Vet. Med., 6, Pinedo, P.J., Buergelt, C.D., Donovan, G.A., Melendez, P., Morel, L., Wu, R., Langaee, T.Y., Rae, D.O., Three Candidate Genes (BoIFNG, TLR4, SLC11A1) Polymorphisms and their Association with Paratuberculosis Infection in Cattle. Preventive Veterinary Medicine Pinedo, P.J., Wang, Ch., Rae, D.O., Li, Y., and Wu, R., Risk haplotype analysis for bovine paratuberculosis. Mammalian Genome. 20: Melendez P., Pinedo P.J., The association between reproductive performance and milk yield in Chilean Holstein cattle. J. Dairy Sci. 90: Pedraza, C., Mansilla, A., Merucci, F., Pinedo, P.J., Contreras, H., Urea milk contents in dairy cows of Bío Bío Region, Chile. Agricultura Técnica. 66: Buergelt, C.D., Williams, E., Monif, R.G., Pinedo, P.J., Decker, H., Intern nested polymerase chain reaction and prenatal detection of Mycobacterium avium Subspecies paratuberculosis (Map) in bovine allantoic fluid and fetuses. J. Appl. Res. Vet. Med. 4, Williams, J. E., Buergelt, C.D., Pinedo P., Monif, G.R.G., Use of blotted tissue impressions for rapid PCR identification of Mycobacterium avium subspecies paratuberculosis. The Paratuberculosis Newsletter (International Association for Paratuberculosis). Book Chapters Applied Statistical Analyses for Dairy Production, pages In: Dairy Production Medicine, Risco & Melendez Ed., 2011, Wiley Blackwell, Oxford UK Animal Health: Tuberculosis. Encyclopedia of Agriculture and Food Systems: 5 volume set by Elsevier Science (ISBN: ). Editor: N. Van Alfen. Selected Conference Presentations Oral presentation in 2014 ADSA-ASAS Joint Annual Meeting (Kansas City MO, 2014). o Dynamics of culling for Jersey, Holstein, and crossbred cows in large multi breed herds, P J Pinedo, A Daniels, J Shumaker, A De Vries Oral presentation in 2014 ADSA ASAS Joint Annual Meeting (Kansas City MO, 2014). o Variation in Toll like Receptor Genes and Susceptibility to Clinical Mastitis in Holstein cows. CM Seabury, K N Galvao, K Lager, P. Pinedo Oral presentation in 2014 ADSA ASAS Joint Annual Meeting (Kansas City MO, 2014). o Effect of an Organic Certified Treatment (Optimum Uterflush ) for Toxic Puerperal Metritis on Cure and Reproductive Performance of Dairy Cows. P J Pinedo, J S Velez, H Bothe, D Merchan, J M Piñeiro, C A Risco Oral presentation in 2013 ADSA ASAS Joint Annual Meeting (Indianapolis, 2013). o Milk components as predictors for ruminal indigestion/acidosis in lactating dairy cows. S. Kirchman, P. J. Pinedo, F. P. Maunsell, C. A. Risco, G. A. Donovan. Oral presentation in the Annual Conference of the American Association of Bovine Practitioners (Milwaukee, USA, 2013) Pablo J. Pinedo June 2014 Page 4

5 o Events occurring during the previous lactation, the dry period,and peripartum as risk factors for early lactation mastitis in cows receiving 2 different intramammary dry cow therapies. Fleming CL, Pinedo P. Risco CA. Oral presentation in the Annual Conference of the American Association of Bovine Practitioners (Milwaukee, USA, 2013) o Genomic variation in innate immunity and susceptibility to uterine diseases in Holstein cows. P.J. Pinedo, K.N. Galvao, C.M. Seabury. Oral presentation in XXVII World Buiatric Congress (Lisbon, Portugal, 2012). o Lymphatic vessel puncture at the bovine udder: feasibility and detection of Mycobacterium avium subsp. paratuberculosis in the lymphatic fluid by PCR Oral presentation in XXVII World Buiatric Congress (Lisbon, Portugal, 2012). o Association between dry period length, bacterial culture, and SCC at dry off and incidence of early lactation mastitis in cows receiving Ceftiofur or Penicillin Dihydrostreptomycin as dry therapy Oral presentation in XXVI World Buiatric Congress (Santiago, Chile, 2010). o Association between length of dry period and subclinical mastitis, milk yield, reproductive performance, and culling during the subsequent lactation of Chilean dairy cows Oral presentation in XX Reunión Internacional sobre Producción de Carne y Leche en Climas Cálidos 7 y 8 Octubre, Mexico. o Economic consequences of summer heat stress in dairy cattle. De Vries, A and Pinedo, P. Oral presentation in the 42nd Annual Convention of the American Association of Bovine Practitioners (Omaha, Nebraska, 2009) o Effect of high somatic cell counts on reproductive performance of Chilean dairy cattle, P.J. Pinedo and C. Risco. University of Florida, Gainesville. Oral presentation in the2009 Annual Meeting of the American dairy Science Association(Montreal, Canada, 2009) o Effect of days open in the previous lactation on the risk of culling or death around calving. P. J. Pinedo and A. De Vries, University of Florida, Gainesville. Oral presentation in the Annual Conference of the American Association of Bovine Practitioners (Vancouver, Canada, 2007) o Genetic Resistance to Johne's Disease in Four Cattle Breeds: A Candidate Gene Case Control Study, Preliminary Results Oral presentation in the Annual Conference of the American Association of Bovine Practitioners (Minnesota, USA, 2006) o Analysis of the Association Between ELISA and Nested PCR on Blood and Milk for Mycobacterium avium subsp. paratuberculosis detection in Holstein Cows Oral presentation in the Annual Food Animal Veterinary Medical Conference (Florida, USA, 2009) o Effect of High Somatic Cell Counts on Reproductive Performance of Chilean Dairy Cattle Oral presentation Risco, C.A. and Pinedo P. Consequences of Uterine Infections on Reproductive Efficiency in Dairy Cattle. Proceedings for Expoleche San Marcos Annual Meeting. San Marcos, Aguascalientes, Mexico, April th, 2011 Poster presentations: o 2013 ADSA ASAS Joint Annual Meeting (Indianapolis, 2013). Association between hematological parameters and gender upon arrival with clinical bovine respiratory disease risk in newly received beef calves. J. T. Richeson, P. J. Pinedo, E. B. Kegley, J. G. Powell, M. S. Gadberry, P. A. Beck, S. M. Falkenberg. Pablo J. Pinedo June 2014 Page 5

6 o North American Conference on Precision Dairy Management. (Rochester, USA. 2013). Milk components as predictors for ruminal indigestion in lactating dairy cows. G.A. Donovan, S. Kirchman, P.J. Pinedo, F.P. Maunsell, C.A. Risco. o XXVII World Buiatric Congress (Lisbon, Portugal, 2012). Field study on the efficacy of activated charcoal with wood vinegar liquid on controlling undifferentiated, naturally occurring diarrhea in dairy calves o XXVI World Buiatric Congress (Santiago, Chile, 2010): Efficacy of a Botanical Preparation for the Intramammary Treatment of Clinical Mastitis on an Organic Dairy Farm Dynamics of bulk tank somatic cell count, fat, protein, and urea nitrogen and their association with fertility in Central Southern Chilean dairy cattle o US Animal Health Association Annual Meeting (USAHA) Comparative IS900 and IS1311 Direct Fecal Mycobacterium avium Subspecies Paratuberculosis Nested PCR Tests: Significance of Disparities J.E. Williams, P.J. Pinedo, G.R.G. Monif. o Annual Conference of the American Association of Bovine Practitioners (Milwaukee, 2012): Lymphatic vessel puncture of the bovine udder: feasibility for sample collection and detection of Mycobacterium avium subsp. paratuberculosis in the lymphatic fluid by PCR o Annual Conference of the American Association of Bovine Practitioners (Albuquerque, NM, 2010): Efficacy of a Botanical Preparation for the Intramammary Treatment of Clinical Mastitis on an Organic Dairy Farm o 10th International Colloquium on Paratuberculosis (Minneapolis, Minnesota, 2009): Comparative IS900 and IS1311 direct fecal Mycobacterium avium subspecies paratuberculosis nested PCR testing: Significance of disparities Association between paratuberculosis infection and general immune status in dairy cattle o Annual Conference of the American Association of Bovine Practitioners (Charlotte, NC, 2008): Genetic Resistance to Johne s Disease In Cattle: Three Candidate Genes o 24 th World Buiatric Congress, Nice, France, 2006 Analysis of the Association between ELISA and nested PCR on Bloodand Milk for Mycobacterium avium Subspecies paratuberculosis Detection in Holstein Cows Characterization of Reproductive Parameters In Central Southern Chilean Holstein Cattle From 1990 To 2004 Bulk Tank Linear Score Somatic Cell Count Dynamics in Central Southern Chilean Holstein Cattle from 1994 to 2004 Characterization of Productive Parameters in Central Southern Chilean Holstein Cattle From 1990 To 2004 Evaluation of Bulk Tank Milk Urea Nitrogen in Central Southern Chilean Holstein Cattle From 1990 To 2004 Awards and Grants 2014: Intervention trial to determine effectiveness of water management as a novel strategy to reduce fecal shedding of Escherichia coli O157:H7 in finishing cattle. Ivanek Miojevic R, Pinedo P. Lawhon S. Formula Animal Health, $40,000. Pablo J. Pinedo June 2014 Page 6

7 TAMU, College of Veterinary Medicine Effect of amniotic sac palpation per rectum for pregnancy diagnosis on pregnancy loss and congenital abnormalities in newborn calves., Romano, J., Pinedo P., Thomson J. Role: Collaborator. Amount Awarded: $12, : Strategies to better target the application of BRD metaphylactic treatment in newly received cattle. P. Pinedo, J Richeson, H. Naikare, W. Pinchak. Texas A&M AgriLife Research Distillers Grains and Beef Competitiveness Research Initiative. 2013: $47,000, 2014: $47,000. Principal Investigator. Awarded. 2013: Association between animal behavior and tympanic temperature with clinical bovine respiratory disease. Co PI. (AABP, American Association of Bovine Practitioners Foundation, $24,490). Awarded. 2013: A behavioral based monitoring system for preclinical detection and mitigation of bovine respiratory disease in beef cattle. G Castrens, W Pinchak, L. Tedeschi, P. Pinedo and J. Co Investigator. Wall. Texas A&M AgriLife Research Distillers Grains and Beef Competitiveness Research Initiative. $159,000. Awarded. 2012: Genomic selection for improved fertility of dairy cows with emphases on cyclicity and pregnancy Integrated research, extension, and education proposal. Translational Genomics for Improved Fertility of Animals, AFRI, NIFA (5 years grant, $2,977, 638). Principal Investigator. Awarded. 2011: Dynamics of culling risk for Jersey, Jersey x Holstein and Holstein cows in large multi breed dairy herds (American Jersey Cattle Association; $5,500). Principal Investigator. Awarded : University of Florida, College of Veterinary Medicine Alumni Fellowship to accomplish the PhD degree. 2007: Richard Merkal Fellowship (International Association for Paratuberculosis) recipient to present research results in the 9th International Colloquium on Paratuberculosis (9ICP) in Tsukuba, Japan. o Association between two polymorphisms in the bovine CARD15/NOD2 gene and paratuberculosis infection in Florida dairy and beef cattle. 2008: College of Veterinary Medicine Excellence in Doctoral Studies Award. 2007: The Florida Milk Check Off Research and Education Program ( Genetic Resistance to Johne s Disease; a Case Control Study, Owen Rae, Claus Buergelt, Elliot Williams, Rongling Wu, Pablo Pinedo). Awarded. 2007: CVM Spring Consolidated Faculty Research Development Award Grant Competition ( Genetic Resistance to Johne s Disease; a Case Control Study, Owen Rae, Claus Buergelt, Elliot Williams, Rongling Wu, Pablo Pinedo). Awarded. 2007: Recognition to Service to the Veterinary Medicine Profession. University of Florida Multicultural Program. Work in Progress Genomic selection for improved fertility of dairy cows with emphasis on cyclicity and pregnancy (in development 5 years). Genomic variation in innate immunity and susceptibility to mastitis in Holstein cows. Effect of a novel treatment of toxic puerperal metritis on cure and subsequent reproductive performance. Association between rumination behavior and health disorders during early lactation. Association between animal behavior and tympanic temperature with clinical bovine respiratory disease. Strategies to better target the application of BRD metaphylactic treatment in newly received cattle. A behavioral based monitoring system for preclinical detection and mitigation of bovine respiratory disease in beef cattle. Pablo J. Pinedo June 2014 Page 7

8 Participation in other Projects 2008: Efficacy of Ceftiofur crystalline free acid sterile suspension (200mg/mL) at 6.6 mg/kg BW for treatment of lactating dairy cattle at high risk of developing acute postpartum metritis following dystocia, stillbirth, twinning and/or retained placenta (1133C ). Professional Memberships American Association of Bovine Practitioners American Dairy Science Association Texas Animal Nutrition Council Languages Spanish native language English fluent Service and Professional Activities Member of the IACUC Review Committee West Texas A&M University Texas A&M AgriLife Research Panel member for the Animal Production and Protection review panel for the USDA Small Business Innovation Research (SBIR Phase I) Program. Ad hoc reviewer for the Animal Production and Protection review panel for the USDA Small Business Innovation Research (SBIR Phase II) program. Ad hoc reviewer for: Animal Reproduction Science Journal J. Dairy Science Research in Veterinary Science Veterinary Immunology and Immunopathology The Veterinary Journal Pablo J. Pinedo June 2014 Page 8

CURRICULUM VITAE POSITION TITLE INSTITUTION AND LOCATION DEGREE YEAR FIELD OF STUDY

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