CURRICULUM VITAE POSITION TITLE INSTITUTION AND LOCATION DEGREE YEAR FIELD OF STUDY

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1 CURRICULUM VITAE POSITION TITLE Pablo J. Pinedo EDUCATION Assistant Professor of Ruminant Animal Health Amarillo Texas AgriLife Research Center Veterinary Medicine & Biomedical Sciences, TAMU INSTITUTION AND LOCATION DEGREE YEAR FIELD OF STUDY CVM University of Florida, Gainesville, Fl PhD 2008 Veterinary Medicine University of Chile, Santiago, Chile D.V.M 1993 Veterinary Medicine Contact Information Phone: e- mail: / ppinedo@ag.tamu.edu Professional Experience 2011 to present Assistant Professor of Ruminant Animal Health Residency, Large Animal Clinical Sciences, University of Florida College of Veterinary Medicine (Food Animal Reproduction and Medicine Service) Postdoctoral position, Department of Animal Sciences, University of Florida. DHIA data analyses to explore the dynamics of culling in dairy systems and its association with diverse risk factors (Dr. Albert De Vries) PhD program, College of Veterinary Medicine. Cumulative UF GPA: 4.0. Program centered on Johne s disease diagnostic, epidemiology, and genetic factors associated to susceptibility to paratuberculosis infection (microsatellite and SNP analyses). Dissertation title: Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection 2007 Teacher Assistant in Dairy Production (UF, CVM) INSECABIO LTDA. (Bovine artificial insemination company, Bioleche Dairy Cooperative, Chile). Dairy Recording System manager and cattle genetics specialist. Assistance to dairy farmers in breeding programs and use of information for management decisions. Management of cattle classification and mating programs Veterinary consultant in San Rafael cow calf operation (Chile) 1994 Veterinary consultant in Rinconada de Tinguiririca dairy farm (Chile) Veterinary School (U. of Chile), Teacher Assistant in Animal Genetics 1993 Best student of the Veterinary School 1993 class, University of Chile 1992 Agronomy School (Catholic University of Chile), Teacher Assistant in Animal Genetics Pablo J. Pinedo Page 1

2 Training and Technical Tours Oct 2007 Dec 2003 May 2003 Oct 2000 Sept 1997 May 1996 Dairy Genetic Showcase (Michigan, Ohio, Wisconsin) and World Dairy Expo (Madison, USA) Genetic tour to New Zealand. Topic: Genetic of composite (crossbreeding systems in bovine and ovine) Dairy tour to France. Topic: Performance of Montbeliarde dairy breed Dairy tour and World Dairy Expo (Madison, USA) Dairy tour to Canada (Montreal Quebec). Topic: Information systems for management and health in dairy herds Dairy tour to United States (Wisconsin California). Topic: Dairy systems and dairy genetics Research Interests/Areas of Expertise Epidemiology and genetics in animal disease Paratuberculosis diagnostic and genetics Preventive dairy production medicine Reproduction in dairy cattle Data management in dairy systems Dairy and beef cattle infectious diseases Selected Peer- reviewed Publications and Book Chapters Johannes L.K., Pinedo P.J., Buergelt C.D., Neumann L., Baumgartner W., Rae D.O., 2011.The collection of lymphatic fluid from the bovine udder and its use for the detection of Mycobacterium avium subsp. paratuberculosis in the cow. J Vet Diagn Invest, 24: Pinedo, P. J., C. Fleming, C.A. Risco. Events occurring during the previous lactation, the dry period, and the peripartum as risk factors for early lactation mastitis in cows receiving two different intramammary dry cow therapies. Accepted for publication (Journal of Dairy Science). Pinedo P.J., Karreman H., Bothe H., Risco C., Velez J. Efficacy of a botanical preparation for the intramammary treatment of clinical mastitis on an organic dairy farm. In review process. Effects of 35% corn wet distillers grains plus solubles in steam- flaked and dry- rolled corn based finishing diets on animal performance, carcass characteristics, beef fatty acid profile, and sensory attributes E. K. Buttrey, K. H. Jenkins, J. B. Lewis, S. B. Smith, R. K. Miller, T. E. Lawrence, F. T. McCollum III, P.J. Pinedo, N. A. Cole, and J. C. MacDonald. In review process. Pablo J. Pinedo Page 2

3 Pinedo, P.J., Risco, C. & Melendez, P., A retrospective study on the association between different dry period lengths and somatic cell counts, milk yield, reproductive performance, and culling in Chilean dairy cows. Journal of Dairy Sci. 94: Pinedo P.J., & De Vries, A Dynamics of culling risk with disposal codes reported by DHI dairy herds. Journal of Dairy Sci. 93: Pinedo, P.J., Webb D. & De Vries, A., Effect of days to conception in the previous lactation on the risk of death and live culling around calving. Journal of Dairy Sci. 93: De Vries, A., Olson, J.D., Pinedo, P.J Reproductive risk factors for culling and productive life in large dairy herds in the eastern United States between 2001 and Journal of Dairy Sci. 93: Pinedo, P.J., Melendez, P., Temporal patterns of bulk tank somatic cell count, fat, protein, and urea nitrogen and their association with fertility in central- southern Chilean dairy cattle. Arch Med Vet. 42, Pinedo, P. P. Melendez, J.A.Villagomez- Cortes, and C. Risco., Effect of High Somatic Cell Counts on Reproductive Performance of Chilean Dairy Cattle. Journal of Dairy Sci. 92: Pinedo, P.J., Buergelt, C.D., Donovan, G.A., Melendez, P., Morel, L., Wu, R., Langaee, T.Y., Rae, D.O Association between CARD15/NOD2 gene polymorphisms and paratuberculosis infection in cattle. Vet Microbiol. 134, Pinedo, P.J., Rae, D.O., Williams, J.E., Donovan, G.A., Melendez, P., Buergelt, C.D., Association among serum ELISA, fecal culture, and nested PCR on milk, blood, and feces for the detection of paratuberculosis infection in dairy cows. Transboundary and Emerging Diseases. 55, Pinedo, P.J., Buergelt, C.D., Monif, R.G., Williams, J.E., Rae, D.O., Mycobacterium avium subspecies paratuberculosis shedding into milk: Correlation of Serum ELISA Titers with DNA Detection in Milk. J Appl Res Vet Med. 6, Pinedo, P.J., Buergelt, C.D., Donovan, G.A., Melendez, P., Morel, L., Wu, R., Langaee, T.Y., Rae, D.O., Three Candidate Genes (BoIFNG, TLR4, SLC11A1) Polymorphisms and their Association with Paratuberculosis Infection in Cattle. Preventive Veterinary Medicine Pinedo, P.J., Wang, Ch., Rae, D.O., Li, Y., and Wu, R., Risk haplotype analysis for bovine paratuberculosis. Mammalian Genome 20: Melendez P., Pinedo P., The association between reproductive performance and milk yield in Chilean Holstein cattle. Journal of Dairy Sci. 90: Pedraza, C., Mansilla, A., Merucci, F., Pinedo, P., Contreras, H., Urea milk contents in dairy cows of Bío- Bío Region, Chile. Agricultura Técnica 66: Buergelt, C.D., Williams, E., Monif, R.G., Pinedo, P., Decker, H., Intern nested polymerase chain reaction and prenatal detection of Mycobacterium avium Subspecies paratuberculosis (Map) in bovine allantoic fluid and fetuses. J Appl Res Vet Med. 4, Williams, J. E., Buergelt, C.D., Pinedo P., Monif, G.R.G., Use of blotted tissue impressions for rapid PCR identification of Mycobacterium avium subspecies paratuberculosis. The Paratuberculosis Newsletter (International Association for Paratuberculosis). Pablo J. Pinedo Page 3

4 Applied Statistical Analyses for Dairy Production, pages In: Dairy Production Medicine, Risco & Melendez Ed., 2011, Wiley Blackwell, Oxford UK Selected Conference Presentations Oral presentation in XXVII World Buiatric Congress (Lisbon, Portugal, 2012). o Lymphatic vessel puncture at the bovine udder: feasibility and detection of Mycobacterium avium subsp. paratuberculosis in the lymphatic fluid by PCR Oral presentation in XXVII World Buiatric Congress (Lisbon, Portugal, 2012). o Association between dry period length, bacterial culture, and SCC at dry- off and incidence of early lactation mastitis in cows receiving Ceftiofur or Penicillin Dihydrostreptomycin as dry therapy Oral presentation in XXVI World Buiatric Congress (Santiago, Chile, 2010). o Association between length of dry period and subclinical mastitis, milk yield, reproductive performance, and culling during the subsequent lactation of Chilean dairy cows Oral presentation in XX Reunión Internacional sobre Producción de Carne y Leche en Climas Cálidos 7 y 8 Octubre, Mexico. o Economic consequences of summer heat stress in dairy cattle. De Vries, A and Pinedo, P. Oral presentation in the 42nd Annual Convention of the American Association of Bovine Practitioners (Omaha, Nebraska, 2009) o Effect of high somatic cell counts on reproductive performance of Chilean dairy cattle, P.J. Pinedo and C. Risco. University of Florida, Gainesville. Oral presentation in the2009 Annual Meeting of the American dairy Science Association(Montreal, Canada, 2009) o Effect of days open in the previous lactation on the risk of culling or death around calving. P. J. Pinedo and A. De Vries, University of Florida, Gainesville. Oral presentation in the Annual Conference of the American Association of Bovine Practitioners (Vancouver, Canada, 2007) o Genetic Resistance to Johne's Disease in Four Cattle Breeds: A Candidate Gene Case Control Study, Preliminary Results Oral presentation in the Annual Conference of the American Association of Bovine Practitioners (Minnesota, USA, 2006) o Analysis of the Association Between ELISA and Nested PCR on Blood and Milk for Mycobacterium avium subsp. paratuberculosis detection in Holstein Cows Oral presentation in the Annual Food Animal Veterinary Medical Conference (Florida, USA, 2009) o Effect of High Somatic Cell Counts on Reproductive Performance of Chilean Dairy Cattle Oral presentation Risco, C.A. and Pinedo P. Consequences of Uterine Infections on Reproductive Efficiency in Dairy Cattle. Proceedings for Expoleche San Marcos Annual Meeting. San Marcos, Aguascalientes, Mexico, April th, 2011 Poster presentations: o XXVII World Buiatric Congress (Lisbon, Portugal, 2012). Field study on the efficacy of activated charcoal with wood vinegar liquid on controlling undifferentiated, naturally- occurring diarrhea in dairy calves o XXVI World Buiatric Congress (Santiago, Chile, 2010): Efficacy of a Botanical Preparation for the Intramammary Treatment of Clinical Mastitis on an Organic Dairy Farm Pablo J. Pinedo Page 4

5 Dynamics of bulk tank somatic cell count, fat, protein, and urea nitrogen and their association with fertility in Central- Southern Chilean dairy cattle o Annual Conference of the American Association of Bovine Practitioners (Albuquerque, NM, 2010): Efficacy of a Botanical Preparation for the Intramammary Treatment of Clinical Mastitis on an Organic Dairy Farm o 10th International Colloquium on Paratuberculosis (Minneapolis, Minnesota, 2009): Comparative IS900 and IS1311 direct fecal Mycobacterium avium subspecies paratuberculosis nested PCR testing: Significance of disparities Association between paratuberculosis infection and general immune status in dairy cattle o Annual Conference of the American Association of Bovine Practitioners (Charlotte, NC, 2008): Genetic Resistance to Johne s Disease In Cattle: Three Candidate Genes o 24 th World Buiatric Congress, Nice, France, 2006 Analysis of the Association between ELISA and nested PCR on Bloodand Milk for Mycobacterium avium Subspecies paratuberculosis Detection in Holstein Cows Characterization of Reproductive Parameters In Central- Southern Chilean Holstein Cattle From 1990 To 2004 Bulk Tank Linear Score Somatic Cell Count Dynamics in Central- Southern Chilean Holstein Cattle from 1994 to 2004 Characterization of Productive Parameters in Central- Southern Chilean Holstein Cattle From 1990 To 2004 Evaluation of Bulk Tank Milk Urea Nitrogen in Central- Southern Chilean Holstein Cattle From 1990 To 2004 Work in Progress The influence of turnover rate of drinking water on the feedlot prevalence of Escherichia coli O157:H7 Analysis of temporal patterns of levels of passive transfer and their association with colostrum quallity, management, and biological variables in a Florida dairy farm Performance of calves fed fresh colostrum from their dams compared to those fed colostrum from non- dams Genomics of the innate immune system and variation for susceptibility to uterine disease and mastitis in dairy cattle Awards and Grants 2012: Genomic selection for improved fertility of dairy cows with emphases on cyclicity and pregnancy Integrated research, extension and education proposal. Translational Genomics for Improved Fertility of Animals, AFRI, NIFA. Awarded. 2011: Dynamics of culling risk for Jersey, Jersey x Holstein and Holstein cows in large multi- breed dairy herds (American Jersey Cattle Association). Awarded : University of Florida, College of Veterinary Medicine Alumni Fellowship to accomplish the PhD degree. Pablo J. Pinedo Page 5

6 2007: Richard Merkal Fellowship (International Association for Paratuberculosis) recipient to present research results in the 9th International Colloquium on Paratuberculosis (9ICP) in Tsukuba, Japan. o Association between two polymorphisms in the bovine CARD15/NOD2 gene and paratuberculosis infection in Florida dairy and beef cattle. 2008: College of Veterinary Medicine Excellence in Doctoral Studies Award. 2007: The Florida Milk Check- Off Research and Education Program ( Genetic Resistance to Johne s Disease; a Case Control Study, Owen Rae, Claus Buergelt, Elliot Williams, Rongling Wu, Pablo Pinedo). 2007: CVM Spring Consolidated Faculty Research Development Award Grant Competition ( Genetic Resistance to Johne s Disease; a Case Control Study, Owen Rae, Claus Buergelt, Elliot Williams, Rongling Wu, Pablo Pinedo). 2007: Recognition to Service to the Veterinary Medicine Profession. University of Florida Multicultural Program. Participation in other Projects 2008: Efficacy of Ceftiofur crystalline free acid sterile suspension (200mg/mL) at 6.6 mg/kg BW for treatment of lactating dairy cattle at high risk of developing acute postpartum metritis following dystocia, stillbirth, twinning and/or retained placenta (1133C ). Professional Memberships American Association of Bovine Practitioners American Dairy Science Association Languages Spanish - native language- English - fluent- Pablo J. Pinedo Page 6