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1 REPUBLIC OF SOUTH AFRICA THE ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH Volume 1985 Numbers 1-4 SUBJECT INDEX Volume Abortion Leptospira interrogans serovar hardjo associated with bovine abortion in South Africa. LESLEY A. TE BRUGGE and TERSIA DREYER Mycoplasmas recovered from bovine genitalia, aborted foetuses and placentas in the Republic of South Africa. C. J. V. TRICHARD and ELSIE P. JACOBSZ African swine fever The epidemiology of African swine fever: The role of free-living hosts in Africa. G. R. THOMSON Amblyomma bebraeum J. L. DUPLESSIS Angora goats J. L. DUPLESSIS Antidorcas rnarsuplalls Arthropods and helminths in springbok (Antidorcas marsupia/is) at Benfontein, Kimberley. I. L. DE VILLIERS, R. LIVER- SIDGE and R. K. REINECKE API 50 CH system Some features of coagulase positive staphylococci from bovine milk. 1. Carbohydrate metabolism: Comparison of conventional techniques and the API 50 CH system. J. A. ERASMUS Argas (Persicargas) wa1kerae An investigation into the emission sites of the volatile pheromone produced by females of Argas (Persicargas) walkerae. R. GOTHE and A. W. H. NEITZ Arsenical dips The effect of arsenical dips on Parafi/ara bovico/a in artificially infected cattle in South Africa. E. M. NEVILL Arthropods Arthropods and helminths in springbok (Antidorcas marsupia/is) at Benfontein, Kimberley. I. L. DE VILLIERS, R. LIVER- SIDGE and R. K. REINECKE Ball3 ruminantium. L. PROZESKY and J. L. DUPLESSIS Besnoltla besnolti Chemotherapy of experimental Besnoitia besnoiti infection in rabbits. VARDA SHKAP, D. T. DE WAAL and F. T. POT- GIETER Biotechnology Biotechnology, viral oncogenesis and jaagsiekte. D. W. VERWOERD Bluetongue virus The use of recombinant DNA technology for the development of a bluetongue virus subunit vaccine. H. HUISMANS Bovine abortion Lepsospira interrogans serovar hardjo associated with bovine abortion in South Africa. LESLEY A. TE BRUGGE and TERSIA DREYER Bovine genitalia Mycoplasmas recovered from bovine genitalia, aborted foetuses and placentas in the Republic of South Africa. C. J. V. TRICHARD and ELSIE P. JACOBSZ Bovine herpesvlruses Studies on bovine herpesviruses. Part l. Isolation and characterization of viruses isolated from the genital tract of cattle. A. THEODORIDIS Bovine leukemia virus Bovine leukaemia virus and enzootic bovine leukosis. A. BURNY, C. BRUCK, Y. CLEUTER, M. MAMMERICKX, G. MARBAIX and D. PORTETELLE Bovine milk Some features of coagulase positive staphylococci from bovine milk. 1. Carbohydrate metabolism. Comparison of conventional techniques and the API 50 CH system. J. A. ERASMUS Brucellosis WILLIAMSON, J. A. ROOS and G. J. SCHIELE

2 Bufadienolides Isolation and characterization of and some observations on poisoning by bufadienolides from Cotyledpn orbiculata L. var. orbiculata. L.A. P. ANDERSON, R. ANITRA SCHULTZ, T. S. KELLERMAN, SARIE M. KOTZE, L. PROZESKY, G. L. ERASMUS and LEONIE LABUSCHAGNE Buffalo Buffalo in the Northern Natal game parks show no serological evidence of infection with foot-and-mouth disease virus. J. J. ESTERHUYSEN, G. R. THOMSON, J. R. B. FLAMMANDandR. G. BENGIS.... Calves An in vivo comparison of the efficacy of the heartwater blood and ground-up tick suspension vaccines in calves. J. D. BEZUIDENHOUT and A. M. SPICKETT.... Carbohydrate metabolism Some features of coagulase positive staphylococci from bovine milk. 1. Carbohydrate metabolism: Comparison of conventional techniques and the API 50 CH system. J. A. ERASMUS.... Cattle The epidemiology and control of heartwater and other tick-borne diseases of cattle in South Africa. J. D. BEZUIDENHOUT... The epidemiology and control of gastriontestinal roundworm infestation of sheep and cattle in South Africa. 1. The historic role ofonderstepoort and a short discussion of present research priorities. J. A. VANWYK.... The effect of arsenical dips on Parafilara bovicola in artificially infected cattle in South Africa. E. M. NEVILL.... Studies on bovine herpesviruses. Part 1. Isolation and characterization of viruses isolated from the genital tract of cattle. A. THEODORIDIS.... The natural resistance of cattle to artificial infection with Cowdria ruminantium: the role played by conglutinin. J. L. DU PLESSIS.... Volume Cellular affinity chromatography Isolation of Codria ruminantium by cellular affinity chromatography and detection by an enzyme-linked immuno-sorbent assay. G. J. VILJOEN, N. M. J. VERMEULEN, P. J. OBEREM, L. PROZESKY, J. A. VERSCHOOR, J.D. BEZUIDENHOUT, J. F. PUTTERILL, L. VISSER and A. W. H. NEITZ {) Chemotherapy Chemotherapy of experimental Besnoitia besnoiti infection in rabbits. VARDA SHKAP, D. T. DE WAAL and F. T. POT- GIETER Commemorative lecture Onderstepoort today, yeasterday and tomorrow. R. D. BIGALKE Complement fixation test technique WILLIAMSON, J. A. ROOS and G. J. SCHIELE Conglutinin The natural resistance of cattle to artificial infection with Cowdria ruminantium: the role played by conglutinin. J. L. DU PLESSIS Cotyledon orblculata var. orbiculata Isolation and characterization of and some observartions on poisoning by bufadienolides from Cotyledon orbiculata L. var. orbiculata. L.A. P. ANDERSON, R. ANITRA SCHULTZ, T. S. KELLERMAN, SARIE M. KOTZE, L. PROZESKY, G. L. ERASMUS and LEONIE LABUSCHAGNE.... Cowdria ruminantium J. L. DUPLESSIS.... The pathology of heartwater. I. A study of mice infected with the Welgevonden strain of Cowdria ruminantium. L. PROZESKY and J. L. DU PLESSIS..... ruminantium. L. PROZESKY and I. L. DUPLESSIS....,..... In vitro cultivation of Cowdria ruminantium. J. D. BEZUIDENHOUT, CAMILLA L. PATERSON and B. J. H. BARNARD... Isolation of Cowdria ruminantium by cellular affinity chromatography and detection by an enzyme-linked immuno-sorbent assay. G. J. VILJOEN, N. M. J. VERMEULEN, P. J. OBEREM, L. PROZESKY, J. A. VERSCHOOR, J.D. BEZUIDEN- HOUT, J. F. PUTTERILL, L. VISSER and A. W. H. NEITZ The natural resistance of cattle to artificial infection with Cowdria ruminantium: the role played by conglutinin. J. L. DU PLESSIS.... Dairy cows The effect of stress on udder health of dairy cows. W. H. GIESECKE Diplodia maydis L. PROZESKY... Diplodiosis L. PROZESKY Distribution of Rhipicephalus glabroscutatum The distribution and hosts of Rhipicephalus glabroscutatum. K. M. MACIVOR {)

3 DNA technology The use of recombinant DNA technology for the development of a bluetongue virus subunit vaccine. H. HUISMANS Domestic ruminants L. PROZESKY Dourine Q fever serology. S. HERR, HILDEGARD F. K. A. HUGHZERMEYER, LESLEY A. TE BRUGGE, CATHERINE C. WILLIAMSON, J. A. ROOS and G. J. SCHIELE Drechslera campanulata SCHNEIDER, W. F. 0. MARASAS, M. G. COLLETT and G. C. A. VANDER WESTHUIZEN Emission sites of pheromones An investigation into the emission sites of the volatile pheromone produced by females of Argas (Persicargas) wa/karae. R. GOTHEandA. W. H. NEITZ EMJHmedium Addition of rabbit serum to EMJH medium improves isolation of Leptospira interrogans serovar hardjo. LESLEY A. TE BRUGGE and H. NICOLETTE LOUW Enzootic bovine leucosis Bovine leukaemia virus and enzootic bovine leukosis. A. BURNY, C. BRUCK, Y. CLEUTER, M. MAMMERICKX, G. MARBAIX and D. PORTETELLE Enzyme-linked immuno-sorbent assay Isolation of Cowdria rimunantium by cellular affinity chromatography and detection by an enzyme-linked immuno-sorbent assay. G. J. VIUOEN, N. M. J. VERMEULEN, P. J. OBEREM, L. PROZESKY, J. A. VERSCHOOR, J. D. BEZUIDEN- HOUT, J. F. PUTTERILL, L. VISSER and A. W. H. NEITZ Epidemiology of African swine fever The epidemiology of African swine fever: The role of free-living hosts in Africa. G. R. THOMSON Epidemiology of heartwater The epidemiology and control of heartwater and other tick-borne diseases of cattle in South Africa. J. D. BEZUIDENHOUT Epidemiology of infectious diseases The epidemiology of infectious diseases of livestock. F. A. MURPHY Epidemiology of Para filaria bovicola The epidemiology of Parafilaria bovicola in the Transvaal Bushveld of South Africa. E. M. NEVILL Epidemiology of roundwonn infestation The epidemiology and control of gastrointestinal roundworm infestation of sheep and cattle in South Africa. 1. The historic role ofonderstepoort and a short discussion of present research priorities. J. A. VANWYK Equine priplasmosis WILLIAMSON, J. A. ROOS and G. J. SCHIELE Farm stock Paralysis and lipofuscin-like pigmentation of farm stock caused by the plant, Trachyandra /axa var. laxa. RINA C. GRANT, P. A. BASSON and A. B. KIDD Fertility Stress and fertility. A review. R. I. COUBROUGH Foetuses Mycoplasmas recovered from bovine genitalia, aborted foetuses and placentas in the Republic of South Africa. C. J. V. TRICHARD and ELSIE P. JACOBSZ Foot-and-mouth disease virus Buffalo in the Northern Natal game park show no serological evidence of infection with foot-and-mouth disease virus. J. J. ESTERHUYSEN, G. R. THOMSON, J. R. B. FLAMMANDandR. G. BENGIS Free-living hosts The epidemiology of African swine fever: The role of free-living hosts in Africa. G. R. THOMSON Fungal pathogen SCHNEIDER, W. F. 0. MARASAS, M. G. COLLETT and G. C. A. VANDER WESTHUIZEN Game parks Buffalo in the Northern Natal game parks show no serological evidence of infection with foot-and-mouth disease virus. J. J. ESTERHUYSEN, G. R. THOMSON, J. R. B. FLAMMAND and R. G. BENGIS Genital tract Studies on bovine herpesviruses. Part I. Isolation and characterization of viruses isolated from the genital tract of cattle. A. THEODORIDIS

4 Goats J. L. DU PLESSIS ruminantium. L. PROZESKY and J. L. DUPLESSIS SCHNEIDER, W. F. 0. MARASAS, M. G. COLLETT and G. C. A. VANDER WESTHUIZEN Ground-up tick suspension vaccines An in vivo comparison of the efficacy of the heartwater blood and ground-up tick suspension vaccines in calves. J. D. BEZUI- DENHOUT and A. M. SPICKETT Guinea-pigs Enrichment of a fraction toxic to guinea-pigs from Pachystigma pygmaeum (Schltr.) Robyns. J. A. VERSCHOOR, H. G. PATTERTON and D. J. J. POTGIETER Heartwater J. L. DUPLESSIS... c The pathology of heartwater. I. A study of mice infected with the Welgevonden strain of Cowdria ruminantium. L. PROZESKY and J. L. DU PLESSIS ruminantium. L. PROZESKY and J. L. DUPLESSIS..., The epidemiology and control of heartwater and other tick-borne diseases of cattle in South Africa. J. D. BEZUIDENHOUT Heartwater blood vaccine An in vivo comparison of the efficacy of the heartwater blood and ground-up tick suspension vaccines in calves. J. D. BEZUI- DENHOUT and A. M. SPICKETT Helminths Arthropods and helminths in springbok (Antidorcas marsupialis) at Benfontein, Kimberley. I. L. DE VILLIERS, R. RIVER- SH>GE and R. K. REINECKE Hepatogenous photosensitivity diseases Hepatogenous photosensitivity diseases in South Africa. T. S. KELLERMAN and J. A. W. COETZER Hepatotoxicosls An ovine hepatotoxicosis caused by the plant Hertia pallens (DC.) Kuntze (Asteraceae). L. PROZESKY, T. S. KELLERMAN, P. JORDAAN, WILHELMINA G. WELMAN and J. P. J. JOUBERT Herpesvlruses Studies on bovine herpesviruses. Part 1. Isolation and characterization of viruses isolated from the genital tract of cattle. A. HEODORIDIS Hertla pallens An ovine hepatotoxicosis caused by the plant Hertia pal/ens (DC.) Kuntze (Asteraceae). L. PROZESKY, T. S. KELLERMAN, P. JORDAAN, WILHELMINA G. WELMANand J.P. J. JOUBERT Hosts of Rhipicephalus glabroscutatum The distribution and hosts of Rhipicephalus glabroscutatum. K. M. MACIVOR Hyperlmmune serum The efficacy of hyperirnmune serum in the treatment of sweating sickness. P: T. OBEREM, G. R. VAN AMSTEL, 0. MATTHEEandJ. D. BEZUIDENHOUT Infectious diseases The epidemiology of infectious diseases of livestock. F. A. MURPHY Intestinal spirochaetosis "Intestinal phirochaetosis" of the vervet monkey. HEATHERM. COWLEY and R. R. HILL In vitro cultivation In vitro cultivation of Cowdria ruminantium. J. D. BEZUIDENHOUT, CAMILLA L. PATERSON and B. J. H. BARNARD Jaagsiekte Biotechnology, viral oncogenesis and jaagsiekte. D. W. VERWOERD Leptospira interrogans serovar hardjo Leptospira interrogans serovar hardjo associated with bovine abortion in South Africa. LESLEY A. TE BRUGGE and TERSIA DREYER Addition of rabbit serum to EMJH medium improves isolation of Leptospira interrogans serovar hardjo. LESLEY A. TE BRUGGE and H. NICOLETTE LOUW Leukemia virus Bovine leukaemia virus and enzootic bovine leukosis. A. BURNY, C. BRUCK, Y. CLEUTER, M. MAMMERICKX, G. MARBAIX and D. PORTETELLE Lipofuscin-like pigmentation Piirillysis 11nd lipofuscin-like pigmentation of fann stock caused by the plant, Trachyandra laxa var. laxa. RINA C. GRANT, P. A. BASSON and A. B. KIDD

5 Lipofuscin storage disease A suspected lipofuscin storage disease of sheep associated with ingestion of the plant, Trachyandra divaricata (Jacq.) Kunth. S. J. NEWSHOLME, D. J. SCHNEIDER and CLARE REID.... Volume Livestock The epidemiology of infectious diseases of livestock. F. A. MURPHY..... Lung lesions ruminantium. L. PROZESKY and J. L. DUPLESSIS.... Male pheromones Investigation into the participation of male pheromones of Rhipicephalus evercsi evercsi during infestaion. R. GOTHE and A. W. H. NEITZ... Mice J. L. DUPLESSIS.... The pathology of heartwater. I. A study of mice infected with the Welgevonden strain of Cowdria ruminamium. L. PROZESKY and J. L. DUPLESSIS.... Morphology of sensory receptor ATTWELL Mycoplasmas Mycoplasmas recovered from bovine genatalia, aborted foetuses and placentas in the Republic of South Africa. C. J. V. TRICHARD and ELSIE P. JACOBSZ Mycotoxicosis SCHNEIDER, W. F. 0. MARASAS, M.G. COLLETT and G. C. A. VANDER WESTHUIZEN.... Neuromycotoxicosis L. PROZESY Nippled tubercles ATTWELL Oats SCHNEIDER, W. F. 0. MARASAS, M. G. COLLETT and G. C. A. VANDERWESTHUIZEN..... Onderstepoort Onderstepoort today, yestarday and tomorrow. R. D. BIGALKE..... The epidemiology and control of gastroitestinal roundworm infestation of sheep and cattle in South Africa. I. The historic role of Onderstepoort and a short discussion of present research priorities. J. A. VAN WYK..... Ovine hepatotoxlcosis An ovine hepatotoxicosis caused by the plant Hertia pal/ens (DC.) Kuntze (Asteraceae). L. PROZESKY, T. S. KELLERMAN, P. JORDAAN, WILHELMINA G. WELMAN andj. P. J. JOUBERT..... Pachystigma pygmaeum Enrichment of a fraction toxic to guinea-pigs from Pachystigma pygmaeum (Schltr.) Robyns. J. A. VERSCHOOR, H. G. PATTERTON and D. J. J. POTGIETER.... Parafilarla bovlcola The effect of arsenical dips on Parafilara biviloca in artificially infected cattle in South Africa. E. M. NEVILL The epidemiology of Parafilaria bovicola in the Transvaal Bush veld of South Africa. E. M. NEVILL..... Paralysis of farm stock Paralysis and lipofuscin-like pigmentation of farm stock caused by the plant, Trachyandra laxa var. laxa. RINA C. GRANT, P. A. SASSON and A. B. KIDD Pathology of heartwater J. L. DU PLESSIS.... The pathology of heartwater. I. A study of mice infected with the Welgevonden strain of Cowdria ruminamium. L. PROZESKY and J. L. DUPLESSIS.... ruminantium. L. PROZESKY and 1. L. DUPLESSIS Pheromone An investigation into the emission sites of the volatile pheromone produced by females of Argas (Persicargas) walkerae. R. GOTHE and A. W. H. NEITZ Photosensitivity diseases Hepatogenous photosensitivity diseases in South Africa. T. S. KELLERMAN and J. A. W. COETZER

6 Placentas Mycoplasmas recovered from bovine genitalia, aborted foetuses and placentas in the Republic of South Africa. C. J. V. TRICHARD and ELSIE P. JACOBSZ Poisoning Isolation and characterization of and some observations on poisoning by bufadienolides from Cotyledon orbiculata L. var. orbiculata. L. A. P. ANDERSON, R. ANITRA SCHULTZ, T. S. KELLERMAN, SARIE M. KOTZE, L. PROZESKY, G. L. ERASMUS and LEONIE LABUSCHAGNE Q fever serology WILLIAMSON, J. A. ROOS and G. J. SCHIELE.... Rabbits Chemotherapy of experimental Besnoitia besnoiti infection in rabbits. VARDA SHKAP, D. T. DE WAAL and F. T. POT- GIETER Rabbit serum Addition of rabbit serum to EMJH medium improves isolation of Leptospira interrogans serovar hardjo. LESLEY A. TE BRUGGE and H. NICOLETTE LOUW Recombinant DNA technology The use of recombinant DNA technology for the development of a bluetongue virus subunit vaccine. H. HUISMANS.... Research priorities The epidemiology and control of gastrointestinal roundworm infestation of sheep and cattle in South Africa. I. The historic role of Onderstepoort and a short discussion of present research priorities. J. A. VAN WYK.... Rhipicephalus evertsi evertsi Investigation into the participation of male pheromones of Rhipicephalus evertsi evertsi during infestation. R. GOTHE and A. W. H. NEITZ Rhipicephalus glabroscutatum The distribution and hosts of Rhipicephalus glabroscutatum. K. M. MACIVOR..... Roundworms The epidemiology and control of gastrointestinal roundworm infestation of sheep and cattle in South Africa. I. The historic role of Onderstepoort and a short discussion of present research priorities. J. A. VANWYK.... Schistosoma mattheei ATTWELL.... Sensory receptor ATTWELL.... Serum The efficacy of hyperinunune serum in the treatment of sweating sickness, P. T. OBEREM, G. R. VAN AMSTEL, 0. MATTHEEandJ. D. BEZUIDENHOUT.... Sheep ruminantium. L. PROZESKY and J. L. DUPLESSIS..... A suspected lipofuscin storage disease of sheep associated with ingestion of the plant, Trachyandra divaricata (Jacq.) Kunth. S. J. NEWSHOLME, D. J. SCHNEIDER and CLARE REID SCHNEIDER, W. F. 0. MARASAS, M. G. COLLETT and G. C. A. VANDER WESTHUIZEN..... The epidemiology and control of gastrointestinal roundworm infestation of sheep and cattle in South Africa. I. The historic role ofonderstepoort and a short discussion of present research priorities. J. A. VANWYK.... Spirochaetosis "Intestinal spirochaetosis" of the vervet monkey. HEATHER M. COWLEY and R. R. HILL.... Sprlngbok Arthropods and helminths in springbok (Antidorcas marsupia/is) at Benfontein, Kimberley. I. L. DE VILLIERS, R. LIVER- SIDGE and R. K. REINECKE Staphylococci Some features of coagulase positive staphylococci from bovine milk. I. Carbohyrate metabolism: Comp~ison of conventional techniques and the API 50 CH system. J. A. ERASMUS Stress Stress-induced diseases and toxicological problems in veterinary science. Stress and fertility. A review. R.I. COUBROUGH.... The effect of stress on udder health of dairy cows. W. H. GIESECKE..... Sweating sickness The efficacy of hyperinunune serum in the treatment of sweating sickness, P. T. OBEREM, G. R. VAN AMSTEL, 0. MATfHEEandJ. D. BEZUIDENHOUT

7 Tick-borne diseases The epidemiology and control of heartwater and other tick-borne diseases of cattle in South Africa. J. D. BEZUIDENHOUT... Tick homogenates J. L. DUPLESSIS Toxic fractions Enrichment of a fraction toxic to guinea-pigs from Pachystigma pygmaeum (Schltr.) Robyns. J. A. VERSCHOOR, H. G. PATIERTON and D. J. J. POTGIETER Trachyanra divaricata A suspencted lipofuscin storage disease of sheep associated with ingestion of the plant, Trachyandra divaricata (Jacq.) Kunth. S. J. NEWSHOLME, D. J. SCHNEIDER and CLARE REID Trachyandra taxa var. taxa Paralysis and lipofuscin-like pigmentation of farm stock caused by the plant, Trachyandra taxa var. taxa. RINA C. GRANT, P. A. BAS SON and A. B. KIDD.... Transvaal Bushveld The epidemiology of Parafilaria bovicola in the Transvaal Bushveld of South Africa. E. M. NEVILL.... Udder health The effect of stress on udder health of dairy cows. W. H. GIESECKE Vaccine The use of recombinant DNA technology forthe development of a bluetongue virus subunit vaccine. H. HUISMANS..... An in vivo comparison of the efficacy of the heartwater blood and ground-up tick suspension vaccines in calves. J. D. BEZUI- DENHOUT and A. M. SPICKETI.... Vervet monkey "Intestinal spirochaetosis" of the vervet monkey. HEATHER M. COWLEY and R. R. HILL.... Veterinary Research Institute Onderstepoort today, yesterday and tomorrow. R. D. BIGALKE Viral oncogenesis Biotechnology, viral oncogenesis and jaagsiekte. D. W. VERWOERD..... Viruses Studies on bovine herpesviruses. Part I. Isolation and characterization of viruses isolated from the genital tract of cattle. A. HEODORIDIS Volatile pheromone An investigation into the emission sites of the volatile pheromone produced by females of Argas (Persicargas) walkerae. R. GOTHE and A. W. H. NEITZ..... Welgevonden strain The pathology of heartwater. I. A study of mice infected with the Welgevonden strain of Cowdria ruminantium. L. PROZESKY and J. L. DU PLESSIS

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