1.1 Molecular Parasitology

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1 1.1 Molecular Parasitology Unit Leader: Julia Walochnik, AssocProf, MSc, PhD Co-workers (in alphabetical order): Elisabeth Dietersdorfer, PhD student Ursula Fürnkranz, Post Doc Susi Glöckl, Tech. Iveta Häfeli, Tech. Nadine Hohensee, Diploma student Christian Husch, Diploma student Martina Köhsler, Post Doc Erik Küng, Diploma student Mellessia Lee, PhD student Verena Mündler, Diploma student Adelheid Obwaller, PhD student Mag. Ute Scheikl, PhD student Thomas Strebinger, Diploma student Martin Weiler, PhD student Employees who left the institute in 2015: New in 2015: Nadine Hohensee, Diploma student Research interests Acanthamoeba infections protozoa as human pathogens protozoa as host cells and vehicles for bacteria protozoan molecular phylogeny anti-protozoan biocides Research programs Ongoing projects

2 Acanthamoeba as host for Burkholderia pseudomallei ERAfrica ( , project leader of Austrian part) Amoebae as vehicles for bacteria TRP209_FWF ( , project leader) Relevance of Viable-But-Non-Culturable Legionellae P24535_FWF ( , project partner) Sandflies and sandfly-borne diseases vector analysis in Eastern Austria BMLVS E90053/60-KA/2013 (project leader, ) Austrian tick populations and tick-borne pathogens BMV E90053/124- KA/2013 (project leader, ) Newly initiated projects Medically relevant trematodes in Austria NHM ( , project partner) Publications 1. Walochnik J, Auer H Häufige und wichtige Parasitosen in Mitteleuropa. Universum Innere Medizin 1: Wylezich C, Walochnik J, Corsaro D, Michel R, Kudryavtsev A Electron microscopical investigations of a new species of the genus Sappinia (Thecamoebidae, Amoebozoa), Sappinia platani sp. nov. reveal a dictyosome for the first time in this genus. Acta Protozool : Standard O 3. Walochnik J, Scheikl U, Haller-Schober EM Twenty years of Acanthamoeba diagnostics in Austria. J Euk Microbiology 62(1):3-11. IF: Standard O 4. Corsaro D, Walochnik J, Köhsler M, Rott MB Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli sp. nov. (genotype T19). Parasitol Res 114(7): IF: Standard O 5. Walochnik J, Aspöck H Sie sind unter uns. Mindestens jeder zweite Mitteleuropäer ist mit Protozoen infiziert. Ärztewoche Medizin 19. Februar: Kiderlen A, Scheid P, Walochnik J, Müller N Amöbenenzephalitiden. Epidemiologisches Bulletin 32: Kiderlen A, Scheid P, Walochnik J, Müller N Acanthamoeba-Keratitis. Epidemiologisches Bulletin 33: Kranzler M, Syrowatka M, Leitsch D, Winnips C, Walochnik J Pentamycin shows high efficacy against Trichomonas vaginalis. Int J Antimicrob Agents Apr;45(4): IF: Top O 9. Lorenzo-Morales J, Khan NA, Walochnik J An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite 22:10. IF: Standard R 10. Oarga Mulec A, Miheli R, Walochnik J, Griessler Bulc T Composting of the solid fraction of blackwater from a separation system with vacuum toilets e Effects on the process and quality. Journal of Cleaner Production xxx (2015) 1e8 IF: Top O

3 11. Michel R, Scheid P, Köhsler M, Walochnik J Acaulopage tetraceros DRECHSLER 1935 (Zoopagales): cultivation, prey pattern and molecular characterization. J Endocyto Cell Res 26: Chomicz L, Conn DB, Padzik M, Szaflik JP, Walochnik J, Zawadzki PW, Pawłowski W, Dybicz M Emerging threats of Acanthamoeba keratitis in Poland: Increasing detection of amoebic isolates resistant to physical and chemical agents. BioMed Research. IF: Standard 13. Mulec J, Dietersdorfer E, Üstüntürk-Onan M, Walochnik J Acanthamoeba and other free-living amoebae in bat guano, an extreme habitat. Parasitol Res Dec 17 IF: Standard O Books and chapters: Cooperations National cooperations within the MUW Department of Spezifische Prophylaxe & Tropenmedizin: Unit: Prof. M. Duchêne: Acanthamoeba encystation; Plant derived extracts with anti-protozoal effects Institute for Hygiene and Applied Immunology (Prof. R. Sommer, Dr. A. Kirschner) Universitätsklinik für Innere Medizin I (Prof. H. Burgmann, Dr. W. Pöppl) National Cooperations outside MUW Prof. M. Horn (Department für Mikrobielle Ökologie, University of Vienna) Doz. A. Indra (AGES, Vienna) Dr. A. Obwaller (Orphanidis Pharma Research, Vienna) Dr. H. Sattmann (Naturhistorisches Museum, Vienna) Prof. S. Schmitz-Esser (Veterinary University, Vienna) Prof. I. Wilson (Department für Chemie, BOKU, Vienna).

4 International cooperations Dr. D. Corsaro (University of Neuchâtel, Switzerland) Prof. Y. Hechard (University of Poitiers, France) Dr. A. Kiderlen (Robert-Koch Institute, Berlin, Germany) Prof. J. Lindo (University of the West Indies, Jamaica) Dr. R. Michel (Central Institute of the Federal Armed Forces Medical Services, Germany) Dr. J. Mulec (Karst Research Institute, Slovenia) Doz. S. Poppert (Bernhard-Nocht Institute, Hamburg, Germany) Prof. I. Steinmetz (Uni Greifswald, Germany) Grants and third party funds See above (projects) Diploma, PhD-thesis, Lectures PhD students Mag. Ute Scheikl Amoebae as vehicles for bacteria Mag. Elisabeth Dietersdorfer Relevance of Viable-But-Non-Culturable Legionellae Mag. Adelheid Obwaller Phlebotomes as vectors for Leishmania and phleboviruses Mag. Martin Weiler Tick-borne pathogens MSc. Astrid Erber Monitoring Ivermectin-Resistance in Onchocerca volvulus MSc. Mellessia Lee VSP gene expression in zoonotic assemblages of Giardia duodenalis

5 Diploma students Nadine Hohensee: Medically relevant trematodes Christian Husch: Genotyping of Austrian Fasciola and Fascioloides isolates Erik Küng: Effectivity of Octenisept against T. vaginalis Verena Mündler: Acanthamoeba as host for B. pseudomallei Thomas Strebinger: Babesiosis in Austria Lectures SSM3 Koordination der Projektstudiengruppe, SE, 2,5 Std SSM3 Projektstudie, , SK, 4 S Std Krankheit, Manifestation und Wahrnehmung, allgemeine Arzneitherapie, , SE+PR, Block 9, 3,53 SStd Med.-parasitologisches Praktikum und Seminar, (= ), SE+UE, 2 SStd TS Journal Club Parasitologie, (= ), SE, 2 SStd TS Grundlagen der molekularen Parasitologie, , VO+UE, 2 SStd MS: Tropenmedizin, , SE, 1 SStd JC "Hot Topics in Immunology", , SE, 2 SStd Wissenschaftliche Arbeiten, , SE, 4 SStd Anleitung zu wissenschaftlichen Arbeiten, , SE, 3 SStd imhai Comparative Protozoal Infections, SE, 0.5 SStd Awards, Functions, Patents President of the Austrian Society of Tropical Medicine and Parasitology (ÖGTP) Vice president of the German Society of Protozoology Board member of the Austrian Society of Biological Systematics (NOBIS) Board Member of the German Society for Parasitology (DGP) Austrian representative (ECDC meeting on mosquito-borne infections) Pool of Scientific advisors the European Commission Auditor of the European Federation of Parasitologists (EFP) Review board for Infect-ERA International Exchange MSc. Mellesia Lee, PhD exchange from Jamaica

6 Referee activity, Editor Acta parasitologica, Acta protozoologica, Acta tropica, Archives of Microbiology, British Journal for Ophthalmology, BMC journals, Emerging Infectious Diseases, Environmental Microbiology, Eukaryotic Cell, European Journal of Clinical Microbiology & Infectious Diseases, European Journal of Protozoology, FEMS Immunology & Medical Microbiology, FEMS Microbiology Ecology, Folia Parasitologica, Infection and Immunity, Journal of Clinical Microbiology, Journal of Medical Microbiology, Parasitology Research, Trends in Parasitology Editor for International Journal of Antimicrobial Agents Editor for Experimental Parasitology Editor for Parasitology Research Editor for The Open Infectious Diseases Journal (TOIDJ) Guest editor for Wiener Klinische Wochenschrift (WKW) Editorial advisory board of: Recent Patents on Anti-Infective Drug Discovery, The Journal of Eukaryotic Microbiology Congresses and Presentations Invited lectures (Julia Walochnik) Leishmaniosen (Bayerische Tropenmedizin-Tagung, Deggendorf, 27. Juni 2015) Miltefosine as an anti-acanthamoeba drug (ECOP, Seville, Spain, 10. Sept 2015) Acanthamoebae (Institute of Pharmacology and Toxicology, Veterinary University, 22. Dec 2015) Lectures (Julia Walochnik) Monitoring Acanthamoeba infections in Austria (Meeting of the German Society of Protozoology, Magdeburg, 7. March 2015) Typical and exceptional cases of Acanthamoeba infections (FLAM meeting, Sardinia, Italy, 20. Mai 2015) Babesia spp. and cases of human babesiosis in Austria (ICLB, Vienna, 30. September 2015) Acanthamoeba encephalitis (ÖGTPM, Vienna, 12. Nov 2015)

7 Amoebae free-living versus parasitic traits (Immunology Research Cluster Meeting, Vienna, 2. Dec 2015)

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