reflux esophagitis RE 100 Wistar mg /kg + mg /kg 7 14 ph 7 P <

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1 CJITWMNovember 204Vol. 34No reflux esophagitis RE 00 Wistar RE mg /kg mg /kg mg /kg 5. 7 g 2. 5 ml RE Effect of Pungent Dispersion Bitter Purgation Method on the Esophageal Mucosal Intercellular Space of Reflux Esophagitis Model Rats TANG Yan-ping LIU Si-miao WEI Wei 2 WEI Xiao-dong GONG Yan-xia WANG Rui LI Shu-hong and LIU Ji-wei Department of Digestive DiseaseTianjin Nankai HospitalTianjin China 2 Department of Digestive DiseaseWangjing HospitalChina Academy of Chinese Medical ScienceBeijing 0002 China ABSTRACT Objective To observe the effect of pungent dispersion bitter purgation method PDBPM on the esophageal mucosal intercellular space of reflux esophagitis RE model rats. Methods Totally 00 Wistar rats were randomly divided into the control group the model group the Western medicine group WM the Chinese medicine group CM 25 rats in each group. Rats in the control group only received switch operation. Rats in the rest three groups received modified partial cardia muscle incision combined pylorus ligation of external parts to prepare the RE rat model. Starting from the 3rd day after operationwm mixture Motilium 3. 2 mg /kg + Omeprazole Capsule 4. 3 mg /kg + Hydrotalcite Tablet 6. 4 mg / kg was administered by gastrogavage to rats in the WM group. Rats in the CM group was administered by gastrogavage with Modified Banxia Xiexin Decoction 5. 7 g /kg 2. 5 ml each timetwice daily for 4 consecutive days. Equal volume of No Tel cb699@ sina. com DOI /CJIM

2 CJITWM November 204Vol. 34No. normal saline was administered by gastrogavage to rats in the control group and the model group. On day 7 and 4 the lower esophagus valuegeneral specimen of mucosa and histopathologic changes were observed. Intercellular spaces of esophageal epithelium were measured for a control study. Results Compared with the same group at day 7 the lower esophagus value increased at day the naked eye integral of esophageal mucosa and intercellular spaces of esophageal epithelium also decreased at day 4 in the CM group and the WM group Compared with the control group at the same time point the lower esophagus value decreased in the model group The naked eye integral of e- sophageal mucosaand intercellular spaces of esophageal epithelium increased in the model group with increased intercellular spaces Compared with the model group at the same time pointthe lower esophagus value increased and the naked eye integral of esophageal mucosa decreased in the CM group and the WM group at day 7 and Intercellular spaces of esophageal epithelium of RE model rats at day 4 was lower in the CM group and the WM group than in the model group Compared with the WM groupthe lower esophagus value decreased at day 7 in the CM group the naked eye integral of esophageal mucosa and intercellular spaces of esophageal epithelium decreased at day 4 in the CM group Conclusions PDBPM had favorable treatment effect on RE model rats. The therapeutic effect was more obvious along with the therapeutic course went by. Its mechanism might be achieved through good repair effect on damaged mucosaincreasing the pressure of esophageal sphincterand inhibiting gastric acid. KEYWORDS relux esophagitis pungent dispersion bitter purgation method lower esophageal value histopathology intercellular space of esophageal epithelium reflux esophagitis RE g /ml RE 70% = 5 70% 3 4 RE h lower esophageal sphincterles 00% 0 mg / RE 20 mg / mg / Wistar 250 ~ 280 g H SCXK H % 25 2 h 7 2 MEDICAL DIGITRAPER MKⅢ /MV meter 0 g 0 g 0 g 8 g 8 g 30 g 30 g 5 g 5 g 9 3 0% XK SYNECTICS Digitra ppertmmkⅢ

3 CJITWMNovember 204Vol. 34No. 337 CTD-Synectics BX60 Olympus 4 Ⅱ 4. 2 Ⅲ h 7 0% 3 ml /kg 25 mm - 2 cm 0. 5 cm 0% - HE RAS mm U 24 h 5 g / 6 SPSS g / 75 x ± s % t 0 0 Ⅰ q P < g /kg 5 ml 3. 2 mg /kg mg /kg mg /kg 5 ml ~ ml cm min

4 CJITWM November 204Vol. 34No. x± s n ± ± ± ± ± n n x± s ± * 0 ⅠⅡⅢ ± ± ± * ± * P < 0. 0 P < ± P < 0. 0 P < ± ± ± 0. 2 * ± ± 0. 9 * / 3 A 7 B 4 C 7 D 4 E 7 F 4 G 7 H 4

5 CJITWMNovember 204Vol. 34No μmx± s n ± ± ± ± ± ± * ± ± * RE 7 A 7 B 4 C 7 D 4 E 7 F 4 G 7 H 4 2 HE 400 A 7 B 4 C 7 D 4 E 7 F 4 G 7 H 4 3 HE 400

6 CJITWM November 204Vol. 34No. RE 8 RE RE RE lower esophageal sphincter LES Cajal 2 3 LES RE RE RE 9 Caviglia R 0 RE 3 Ribolsi M 7 RE 8 86% 45% 9 RE Cajal RE

7 CJITWMNovember 204Vol. 34No. 34 RE P 8. J RE 2 J Caviglia RRibolsi MMaggiano Net al. Dilated intercellular spaces of esophageal epithelium in nonerosive reflux disease patients with physiological esophageal acid exposure RE J. Am J Gastroenterol Ribolsi MPerrone GCaviglia Ret al. Intercellular. microscopy analysis J. Dig Liver Dis J J J J J J J J. 6. J J. J M. J J space diameters of the oesophageal epithelium in NERD patients head to head comparison between light and electron 檴檴檴 檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴檴