PESTICIDES KIT 135 COMPONENTS

Transcription

1 Kits Ready to Use

2 Laszlo Hollosi, Katerina Bousova, Michal Godula Thermo Fisher Scientific, Food Safety Response Center, Dreieich, Germany 1. Weigh 10 g sample in 50 ml extraction tube 2. Add 9.8 ml acetonitrile (20 ml water + 10 ml acetonitrile for wheat flour) and 200 μl stock IS 3. Shake for 10 min, centrifuge at 5000 rpm for 5 min 4. Transfer supernatant into a 15 ml clean-up tube 5. Centrifuge samples at 5000 rpm for 5 min 6. Transfer supernatant into a GC vial 7. Add 50 μl sorbitol and 20 μl of injection standard PESTICIDES KIT 135 COMPONENTS KIT4T3L135 Acetone / Acetonitrile / Toluene 13 x 1,1 ml amber silanized ampoules CAS CAS CAS CAS 4,4 -DDD 4,4 -DDE 4,4 -DDT 4,4 -Dichlorobenzophenone Acrinathrin Amitraz BHC-Alpha BHC-Beta Bifenthrin Bromopropylate Bromucanozole Cadusafos Captan Carboxin Chlorfenapyr Chlorfenvinphos Chlorobenzilate Chlorothalonil Chlorpropham Chlorpyrifos-methyl Cyfluthrin, beta- Cyhalothrin-Lambda Cypermethrin Cyproconazole Cyprodinil Deltamethrin Demeton-S-methyl Desmethyl-pirimicarb Diazinon Dichlofluanid Dichlorvos Dicloran Diphenylamine Endosulfan Endosulfansulfate EPN Ethion Ethoprophos Etofenprox -sulfone -sulfoxide Fenitrothion Fenpropathrin Fenpropidin Fenpropimorph Fenvalerate Fipronil Fludioxonil Flutolanil Fluvalinate-Tau Folpet HCH-Gamma Iprodione Isofenphos-methyl Kelthane (Dicofol) Linuron Malathion Mepanipyrim Metalaxyl Methacrifos Methidathion Metribuzin Myclobutanil Orthophenylphenol Oxadiazon Paraoxon-methyl Parathion Parathion-methyl Pendimethalin Permethrin Phenthoate Phosalone Phosmet Phosphamidon Procymidone Profenofos Propargite Propyzamide Prothiofos Pyridaben Pyriproxyfen Spirodiclofen Tefluthrin Tetradifon Tetrahydrophthalimide Tolclofos-methyl Tolylfluanid Trifluralin Triticonazole Vinclozolin Validation of the Method for Determination of Pesticide Residues by Gas Chromatography Triple-Stage Quadrupole Mass Spectrometry Method Validation Method n Thermo 135 Pesticides Key Words TraceFinder, TSQ, Chromatography, GC, GC-MS, Pesticide Residues, QuEChERS, Triple Quadrupole 1. Schematic of Method Homogenization Sample + IS Extraction Clean up GC-MS/MS 2. Introduction Pesticide residue analysis in food is one of the most important and challenging tasks in routine laboratory practice. The European legislation, which is currently the most strict legislation (European Regulation 396/2005 and Commission Directive 2006/125/EC), sets maximum residue limits (MRL) of pesticides in different products of plant and animal origin. This presents a significant analytical challenge with respect to the low limits of quantification (LOQ) required for some specified food matrices. A variety of GC and HPLC methods have been developed for multi-residue determination of pesticides employing a variety of sample preparation and cleanup techniques. In recent years the QuEChERS method has become widely adopted for preparing samples of fruit and vegetables, but the continuous need for more sensitive and accurate measurements requires new developments from the instrument producers as well. Sfoglia e scarica il PDF del Validation Method n Thermo 135 Pesticides su 2

3 3 PESTICIDES KIT 213 COMPONENTS 2,4 -DDD 2,4 -DDE 2,4 -DDT 4,4 -DDD 4,4 -DDE 4,4 -DDT 4,4 -Dichlorobenzophenone Aclonifen Acrinathrin Aldrin Amitraz Anilazine Atrazine Azinphos-ethyl Benfluralin Benfuracarb Benzoylprop-ethyl BHC-Alpha BHC-Beta BHC-Delta Bifenox Bifenthrin Bromophos-ethyl Bromophos-methyl Bromopropylate Bromoxynil-octanoate Bromucanozole Butylate Cadusafos Captan Carbophenothion Carboxin Chinomethionat Chlorfenapyr Chlorfenson Chlorfenvinphos Chlormephos Chlorobenzilate Chloropropylate Chlorothalonil Chlorpropham Chlorpyrifos-methyl Chlorthal-dimethyl Chlozolinate Clomazone Cyanazine Cyfluthrin, beta- Cyhalothrin-Lambda Cypermethrin Cyproconazole Cyprodinil Deltamethrin Demeton-S-methyl Desmethyl-pirimicarb Diazinon Dichlofluanid Dichlorvos Diclofop-methyl Dicloran Dieldrin Diphenylamine Disulfoton Endosulfan Endosulfan-Alpha Endosulfan-Beta Endosulfansulfate Endrin EPN Esfenvalerate Ethion Ethoprophos Ethoxyquin Etofenprox Fenamidone -sulfone -sulfoxide Fenhexamid Fenitrothion Fenothiocarb Fenpropathrin Fenpropidin Fenpropimorph Fenson Fenvalerate Fipronil Fluazinam Flucythrinate Fludioxonil Flutolanil Fluvalinate-Tau Folpet Fonofos HCH-Gamma Heptachlor Heptachlorepoxid, trans- Heptenophos Hexachlorobenzene Iodofenphos Iprodione Isofenphos Isofenphos-methyl Isoxaflutole Kelthane (Dicofol) Linuron Malathion Mepanipyrim Metalaxyl Methacrifos Methidathion Methoxychlor Metribuzin Mevinphos Myclobutanil Napropamide Nitrofen Nitrothal-isopropyl Omethoate Orthophenylphenol Oxadiazon Oxyfluorfen Paraoxon Paraoxon-methyl Parathion Parathion-methyl Penconazole Pendimethalin Permethrin Perthane Phenthoate Phorate Phosalone Phosmet Phosphamidon Pirimiphos-ethyl Procymidone Profenofos Prometryn Propargite Propazine Propyzamide Prothiofos Pyrazophos Pyridaben Pyridaphenthion Pyriproxyfen Quinalphos Quintozene Simazine Simetryn Spirodiclofen Sulfotep Tecnazene Tefluthrin Terbumeton Terbuthylazine Terbutryn Tetrachlorvinphos Tetradifon Tetrahydrophthalimide Thionazin Tolclofos-methyl Tolylfluanid Triazophos Trifluralin Triticonazole Vamidothion Vinclozolin Zoxamide CAS CAS CAS CAS 16 x 1,1 ml amber silanized ampoules Acetone / Acetonitrile / Toluene KIT4T3L213

4 4 PESTICIDES KIT 225 COMPONENTS Abamectin Acetamiprid Acibenzolar-S-methyl Alanycarb Aldicarb Aldicarb-sulfone Aldicarb-sulfoxide Ametryn Aminocarb Amitraz Azinphos-ethyl Benalaxyl Bendiocarb Benfuracarb Benzoximate Bifenazate Bromucanozole Butafenacil Butocarboxim Butoxycarboxim-sulfone Carbendazim Carbetamide, 3-Hydroxy Carbophenothion Carboxin Carfentrazone-ethyl Chlorantraniliprole Chlorfluazuron Chlorotoluron Chloroxuron Clethodim Clofentezine Clothianidin Cyazofamid Cycluron Cymoxanil Cyproconazole Cyprodinil Cyromazine Desmedipham Dichlorvos Diclobutrazol Dicrotophos Diethofencarb Diflubenzuron Dimefuron Dimoxystrobin Diniconazole Dinotefuran Dioxacarb Disulfoton Diuron Doramectin Emamectin-benzoate Eprinomectin Etaconazole Ethiofencarb Ethion Ethiprole Ethirimol Ethofumesate Etoxazole Famoxadone Fenamidone Fenazaquin Fenhexamid Fenobucarb Fenpropimorph Fenpyroximate Fenuron Fipronil Flonicamid Fluazinam Flubendiamide Fludioxonil Flufenacet Flufenoxuron Fluometuron Fluoxastrobin Flutolanil Fonofos Forchlorfenuron Formetanate HCl Fuberidazole Furalaxyl Furathiocarb Halofenozide Hexaflumuron Hexythiazox Hydramethylnon Imidacloprid Indoxacarb Ipconazole Iprovalicarb Isocarbophos Isoprocarb Isoproturon Ivermectin Linuron Lufenuron Malaoxon Malathion Mandipropamid Mefenacet Mepanipyrim Mepronil Mesotrione Metaflumizone Metalaxyl Metconazole Methabenzthiazuron Methomyl Methoprotryne Methoxyfenozide Metobromuron Metribuzin Mevinphos Mexacarbate Monolinuron Moxidectin Myclobutanil Neburon Nitenpyram Novaluron Nuarimol Omethoate Oxamyl Parathion Penconazole Pencycuron Phenmedipham Phorate Picoxystrobin Piperonyl butoxide Pirimiphos-ethyl Promecarb Prometon Prometryn Propamocarb Propargite Propham Propoxur Prosulfocarb Prothioconazole Pymetrozine Pyracarbolid Pyridaben Pyriproxyfen Quinalphos Rotenone Secbumeton Siduron Simetryn Spinetoram Spinosad Spirodiclofen Spiromesifen Spirotetramat Spiroxamine Sulfentrazone Sulfotep Tebuthiuron Teflubenzuron Temephos Terbufos Terbumeton Terbutryn Thiacloprid Thiamethoxam Thidiazuron Thiobencarb Thiofanox Thiophanate-methyl Trichlorfon Tricyclazole Triflumizole Triflumuron Triticonazole Vamidothion Zoxamide CAS CAS CAS CAS 10 x 1,1 ml amber silanized ampoules Acetonitrile KIT4B3L225

5 5 PESTICIDES KIT 274 COMPONENTS 2,4-D Abamectin Acetamiprid Acibenzolar-S-methyl Alachlor Aldicarb-sulfone Allethrin Ametryn Aminocarb Ancymidol Anilofos Aramite Atrazine Azaconazole Azamethiphos Azinphos-ethyl Bendiocarb Benodanil Benoxacor Bensulfuron-methyl Bentazone Benzoximate Benzoylprop-ethyl Bifenazate Brodifacoum Bromacil Bromoxynil Bromuconazole Butachlor Butafenacil Butocarboxim-sulfoxide Butoxycarboxim Carbendazim Carbetamide, 3-Hydroxy Carfentrazone-ethyl Carpropamid Chlorantraniliprole Chlorbromuron Chlorfenvinphos Chlorfluazuron Chloridazon Chlormequat Chlorotoluron Chloroxuron Cinosulfuron Clethodim Clomazone Clothianidin Coumaphos Crotoxyphos Cumyluron Cyanazine Cyazofamid Cycloate Cycluron Cyflufenamid Cyromazine Demeton-S-methylsulfone Desmedipham Desmethyl-pirimicarb Desmetryn Diclobutrazol Dicrotophos Diethofencarb Difenacoum Diflubenzuron Dimefuron Dimethametryn Dimethenamid Dimoxystrobin Diniconazole Dinotefuran Dithiopyr Diuron DNOC Dodemorph Esprocarb Etaconazole Ethiofencarb Ethiofencarb-sulfone Ethiofencarb-sulfoxide Ethiprole Ethirimol Ethofumesate Ethoxyquin Etofenprox Etoxazole Etrimfos Fenamidone Fenazaquin Fenhexamid Fenobucarb Fenoxanil Fenpyroximate Fensulfothion -sulfoxide Fenuron Flazasulfuron Florasulam Fluazifop Fluazinam Flubendiamide Flufenacet Flufenoxuron Flumetsulam Fluometuron Fluopicolide Fluopyram Fluoxastrobin Flurochloridone Forchlorfenuron Formetanate HCl Formothion Fosthiazate Fuberidazole Furathiocarb Griseofulvin Halofenozide Haloxyfop Haloxyfop-methyl Heptenophos Hexaflumuron Hexazinone Hexythiazox Imazaquin Imazethapyr Imibenconazole Imidacloprid Indoxacarb Ioxynil Iprovalicarb Isocarbophos Isoprocarb Isoprothiolane Isoproturon Isoxaben Isoxadifen-ethyl Lenacil Malaoxon Mandipropamid MCPA Mefenacet Mepiquat chloride Mepronil Metamitron Metazachlor Metconazole Methabenzthiazuron -sulfone -sulfoxide Methomyl Methoprotryne Methoxyfenozide Metobromuron Metolachlor Metolcarb Metosulam Metoxuron Metrafenone Metsulfuron-methyl Mevinphos Mexacarbate Monolinuron Napropamide Neburon Nicosulfuron Nuarimol Ofurace Omethoate Oxamyl Penconazole Pencycuron Phenmedipham Phenthoate Phoxim Picoxystrobin Piperonyl butoxide Piperophos Primisulfuron-methyl Profenofos Promecarb Prometon Prometryn Propamocarb Propazine Propetamphos Propoxur Propyzamide Prosulfocarb Pymetrozine Pyroxsulam Quizalofop-ethyl Quizalofop-P Resmethrin Rimsulfuron Rotenone Schradan Sethoxydim Simeconazole Simetryn Spinosad Spiromesifen Spirotetramat Spiroxamine Sulfotep Sulprofos CAS CAS CAS CAS Acetonitrile KIT4B3L x 1,1 ml amber silanized ampoules

6 Katerina Bousova 1, Ebru Sarikaya 1, Michal Godula 1, Claudia Martins 2, and Ed George 2 1 Thermo Fisher Scientific, Special Solution Center Europe, Dreieich, Germany 2 Thermo Fisher Scientific, San Jose, California 1. Weigh 10 g sample in 50 ml extraction tube. 2. Add 10 ml acetonitrile (20 ml water + 10 ml acetonitrile for wheat flour). 3. Shake it for 10 min and centrifuge at 5000 rpm for 5 min. 4. Transfer supernatant into LC vial and place it in the autosampler. Figure 1. Workflow overview. Homogenization Sample Weighing Extraction LC-MS/MS CAS CAS CAS CAS Tebuthiuron Teflubenzuron Tepraloxydim Terbumeton Terbuthylazine Terbutryn Tetramethrin Thiacloprid Thiamethoxam Thidiazuron Thiobencarb Thiophanate-methyl Tolfenpyrad Tralkoxydim Triazophos Trichlorfon Tricyclazole Tridemorph Trietazine Triflumizole Vamidothion Zoxamide Fast Screening and Quantification of Pesticide Residues Using a Comprehensive LC-MS Solution: The Pesticide Explorer Collection Standard Quantitation Application Note 643 Application Note n 643 Thermo 274 Pesticides Key Words Pesticide Explorer Collection, European Regulation 396/2005, Commission Directive 2006/125/EC, European Commission 2002/657/EC, SANCO/12571/2013, European Commission 788/2012/EC, pesticide, food, QuEChERS, UltiMate 3000, TSQ Endura, TraceFinder Goal To present a fully tested LC-MS/MS worklow for rapid and robust quantiication of more than 250 pesticides below maximum residue limits (MRLs) with sensitivity, accuracy, and precision that meets stringent EU guidelines. Introduction Pesticides are chemicals used on crops to protect them from the negative activity of pests. Inappropriate application of pesticides can have adverse effects on health; therefore, determination and quantiication of pesticide residues in foods and food products is an important part of routine food control. The European Union (EU) legislation (European Regulation 396/2005 and Commission Directive 2006/125/EC) requires an extensive and comprehensive study determining pesticides in various products of plant and animal origin. The requirements for low limits of quantiication (LOQ) of pesticides pose signiicant analytical challenges, especially for some complicated food matrices. This study presents a multi-residue analysis method enabled by Thermo Scientiic Pesticide Explorer Collection Standard Quantitation Solution, comprising liquid chromatography triple-stage mass spectrometry (LC-MS/MS), for rapid and robust quantitation of more than 250 pesticides below their required maximum residue limits (MRL). This comprehensive solution includes the Thermo Scientiic QuEChERS sample preparation kit, Dionex UltiMate 3000 LC system, TSQ Endura triple quadrupole mass spectrometer, TraceFinder software, Accucore aq column, and method parameters to provide a start-to-inish worklow for pesticide analysis. The method results address the stringent EU guidelines concerning sensitivity, accuracy and precision. Experimental Overview The worklow overview from sample preparation through LC-MS/MS analysis is shown in Figure 1. Samples were homogenized and extracted according to the European EN QuEChERS protocol prior to injection into the LC-MS/MS system. 1,2 The ready-to-use QuEChERS sample preparation kit containing extraction tubes and associated protocol was used for sample preparation. Identiication of pesticide residues was based on retention time, the presence of a minimum of two product ions, and ion-ratio conirmation using selected reaction monitoring (SRM) of characteristic transition ions. Quantiication was calculated using matrix-matched calibration. All method performance criteria were established according to the relevant EU guidelines. 3-7 Sfoglia e scarica il PDF dell Application Note n 643 Thermo 274 Pesticides su 6

7 7 Cod. KIT4B3L225 Cod Cod Restek - LC Multiresidue Pesticide Kit - Kit 225 LC idquant - Standards Kit AB SCIEX Abamectin Acetamiprid Acibenzolar-S-methyl Alanycarb Aldicarb Aldicarb-sulfone Aldicarb-sulfoxide Ametryn Aminocarb Amitraz Azinphos-ethyl Benalaxyl Bendiocarb Benfuracarb Benzoximate Bifenazate Bromucanozole Butafenacil Butocarboxim Butoxycarboxim-sulfone Carbendazim Carbetamide, 3-Hydroxy Carbophenothion Carboxin Carfentrazone-ethyl Chlorantraniliprole Chlorfluazuron Chlorotoluron Chloroxuron Clethodim Clofentezine Clothianidin Cyazofamid Cycluron Cymoxanil Cyproconazole Cyprodinil CAS CAS Restek Restek idquant idquant Cyromazine Desmedipham Dichlorvos Diclobutrazol Dicrotophos Diethofencarb Diflubenzuron Dimefuron Dimoxystrobin Diniconazole Dinotefuran Dioxacarb Disulfoton Diuron Doramectin Emamectin-benzoate Eprinomectin Etaconazole Ethiofencarb Ethion Ethiprole Ethirimol Ethofumesate Etoxazole Famoxadone Fenamidone Fenazaquin Fenhexamid Fenobucarb Fenpropimorph Fenpyroximate Fenuron Fipronil Flonicamid Fluazinam Flubendiamide Fludioxonil Flufenacet Flufenoxuron PESTICIDES KIT 225 COMPONENTS CROSS-REFERENCE 10 x 1,1 ml amber silanized ampoules Acetonitrile KIT4B3L225

8 8 CAS CAS Restek Restek idquant idquant Fluometuron Fluoxastrobin Flutolanil Fonofos Forchlorfenuron Formetanate HCl Fuberidazole Furalaxyl Furathiocarb Halofenozide Hexaflumuron Hexythiazox Hydramethylnon Imidacloprid Indoxacarb Ipconazole Iprovalicarb Isocarbophos Isoprocarb Isoproturon Ivermectin Linuron Lufenuron Malaoxon Malathion Mandipropamid Mefenacet Mepanipyrim Mepronil Mesotrione Metaflumizone Metalaxyl Metconazole Methabenzthiazuron Methomyl Methoprotryne Methoxyfenozide Metobromuron Metribuzin Mevinphos Mexacarbate Monolinuron Moxidectin Myclobutanil Neburon Nitenpyram Novaluron Nuarimol Omethoate Oxamyl Parathion Penconazole Pencycuron Phenmedipham Phorate Picoxystrobin Piperonyl butoxide Pirimiphos-ethyl Promecarb Prometon Prometryn Propamocarb Propargite Propham Propoxur Prosulfocarb Prothioconazole Pymetrozine Pyracarbolid Pyridaben Pyriproxyfen Quinalphos Rotenone Secbumeton Siduron Simetryn Spinetoram Spinosad Spirodiclofen Spiromesifen Spirotetramat Spiroxamine Sulfentrazone Sulfotep Tebuthiuron Teflubenzuron Temephos Terbufos Terbumeton Terbutryn Thiacloprid Thiamethoxam Thidiazuron Thiobencarb Thiofanox Thiophanate-methyl Trichlorfon Tricyclazole Triflumizole Triflumuron Triticonazole Vamidothion Zoxamide

9 alessandronitti graphicdesigner Download certificates Accreditations and Certifications ISO Guide 34:2009 ISO/IEC 17025:2005 UNI EN ISO 14001:2004 UNI EN ISO 9001:2008 Produced by: LAB. INSTRUMENTS S.r.l. - High Pure Standards & Chemicals Division Tel Fax: SS 172 (Putignano-Alberobello) km Castellana Grotte (BA) Site: info@labinstruments.it,,, and are trademarks of Lab.Instruments srl (Italy). All rights reserved. Specifications, term and pricing are subject to change. Not all products is available in all countries. Please consult your local rapresentative to details. LMM 01 E 2/16