EFFECTS OF MATERNAL BODY WEIGHT, SOME BLOOD PARAMETERS AND EGG QUALITY TRAITS ON FERTILITY, HATCHABILITY AND CHICK WEIGHT OF JAPANESE QUAIL

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1 Egypt. Poult. Sci. Vol (31) (IV): ( ) EFFECTS OF MATERNAL BODY WEIGHT, SOME BLOOD PARAMETERS AND EGG QUALITY TRAITS ON FERTILITY, HATCHABILITY AND CHICK WEIGHT OF JAPANESE QUAIL By Hanan A. Hassan Poult. Prod. Dep., Fac. of Agric., Fay. Univ., Fayoum, Egypt..Received: 02/09/2011 Accepted: 20/10/2011 Abstract: A total number of one hundred and fifty-three females and males of Japanese quail at 14 weeks of age were used with sex ratio 2 female / 1 male to study the effect of maternal body weight, some blood parameters and egg quality traits on fertility% (F%), hatchability% for total and fertile eggs (H/TE % and H/FE %), and chick weight (CW). Birds classified into three groups (light, medium and heavy) according to the body weight at six weeks of age (BW 6 ), (seventeen replicates of each group) and the averages were 205.9, and g of females and were 179.8, and g of males. The results obtained as follows: The eggs weight (EW) and CW increased significantly with increasing the BW 6. The medium group had the highest value of H/TE %. Medium and heavy groups had higher plasma calcium (Ca), total lipids (TL), triglycerides (TG), Red blood cells (RBCs) count than the light group. The heavy female group had the highest significant value of white blood cells (WBCs) count, hemoglobin (Hb), egg surface area (ESA), shell weight / unit surface area (SWUS), egg length (E L), egg width (E WD), albumen weight (Alb W), shell weight (SW) and shell thickness (ST) while it had significant decrease in Haugh unit compared to the other groups. There were significant positive phenotypic correlations between F % and each of plasma Alb, Ca, TL, TG, SWUS, yolk % (Y%) and shell % (S%) and negatively with albumen % (Alb%). The H/TE % positively correlated with each of BW 6, plasma Alb, Ca, TL, TG, RBCs count and yolk diameter (YD), but it correlated negatively with plasma P, glucose, alanine aminotransferase (ALT) and Alb %. The H/FE % correlated positively with each of plasma TP, Ca, RBCs count, WBCs count, Hb %, packed cell volume% (PCV %) and YD and negatively with P, glucose, ALT and yolk index (YI). Significant positive phenotypic correlations were found between CW and each of BW 6, plasma TP, Alb, Ca, TL, TG, aspartate aminotransferase (AST), RBCs count, WBCs count, Hb %, PCV %, EW, ESA, EL, Alb W, SW, SWUS, yolk weight (YW) and ST, but it correlated negatively with plasma P, glucose, ALT and Y%. It can be concluded that the medium and heavy Japanese quail females had some blood parameters and egg quality traits caused improving in each of F%, H/TE%, H/FE% and CW. Therefore, the selection for high body weight or for high some blood parameters may be cause good effects on studied traits. Key Words: Maternal Body Weight, Blood Parameters, Egg Quality, Hatchability, Chick Weight, Japanese Quail INTRODUCTION Recently, in Egypt and other countries, it has been observed in the poultry breeding that the quails were benefited as much as hens both for their meat and eggs, therefore, commercial quail breeding have became widespread. The uniformity of flock at the beginning and during the laying period is the main factor to increase egg production (Leeson et al., 1997). Also, Yalçin et al. (1996) found that hatch weight of Japanese quail increased with increasing the maternal body weight. And the

2 fertility % is affected by factors originating from the hen such as her ability to mate successfully, to store sperm, to ovulate an egg cell and to produce a suitable environment for the formation and development of the embryo (Brillard 2003). Fertility% also depends on hen mate s ability to mate successfully, quantity and quality of semen deposited. The dam that laid the egg was the main source of genetic variation in hatch of fertile eggs, suggesting a huge impact of egg quality traits (Wolc and Olori 2009). External and internal quality traits of the eggs are significant in the poultry breeding for their influence on the yield features of the future generations, breeding performances, and chicks quality and growth (Altinel et al., 1996). Also, Cavero and Schmutz (2009) reported that selection for better hatchability will be in line with not only improved external egg quality, but also internal egg quality like the proportion of yolk in the analysed lines of White Leghorn, but there was antagonism between high Haugh units and good hatchability. Thickness of eggshell is an indirect index of its strength and the eggshell quality (weight, thickness and structure) is the most essential morphological trait for the normal incubation (Rodriguez-Navarro et al., 2002). Calcium and phosphorous are essential macro minerals with calcium forming a significant component of the shell and phosphorous playing an important role in skeletal calcium deposition and subsequent availability of calcium for egg shell formation during the dark period (Boorman et al., 1989). At the onset of egg production, the flow of TG from liver to peripheral tissues, such as muscle and adipose tissues, is redirected mainly to yolk deposition (Bacon 1994). Little information was found on the effects of maternal weight, blood parameters and egg quality traits on F%, H/TE %, H/FE % and CW of quail. Therefore, the present study was conducted to fill this gap. MATERIALS AND METHODS Birds and husbandry The experimental work of this study was carried out at the Poultry Research Station, Poultry Department, Faculty of Agriculture, Fayoum University from March to June A total number of one hundred and fifty-three females and males of Japanese quail at 14 weeks of age were used in this study. Birds were classified into three groups (light, medium and heavy) according to the body weight at six weeks of age, and the averages were , and g of females and were , and g of males, respectively. Birds were marked by wing bands and housed in individual cages with sex ratio 1 male / 2 females (seventeen replicates of each group). Birds were fed on layer diet (19.92% CP, and 2875 kcal ME/kg), and both feed and water were provided ad libitum throughout the experimental period. Artificial light was used beside the normal daylight to provide 17- hour day photoperiod. Sampling and analysis Six hundred eggs that represented the three groups of female s body weight were collected during 14 weeks of age. Eggs were collected at 5.00 pm every day for week in room at C (the eggs of each female were marked with permanent marker) after that incubated in automatic electrical incubator (El Takamoly Poultry Project) at ºF and 60-70% relative humidity and turning each two-hour. After 14 th day of incubation, eggs of each female put in netting plastic and transferred to the hatcher (turning was stopped, 96-97ºF and 80-90% relative humidity). The F %, H/TE %, H/FE % and CW at hatch were determined. Thereafter, at the same age ninety eggs were collected of the last two days (30 eggs of each group of females body weight) to determine the egg quality traits. EW recorded individually, length and width of eggs were measured using caliper to calculate shape index using the formula of Carter (1968), shape index = 706

3 (E WD/EL)*100. Then, eggs were broken on table with a flat glass, yolk diameter measured by caliper, a 3- legged micrometer was used for measuring the height of yolk and albumen. Yolk index was estimated from ratio of yolk height to yolk diameter. Shell thickness was measured in three different parts (sharp, blunt and equatorial) by micrometer after it dried (Tyler 1961). The surface area (cm 2 ) of each egg was estimated using the formula of Carter (1975a) as x W where W is egg weight in grams. Shell weight per unit surface area (mg/ cm 2 ) = shell weight (mg) /egg surface area (cm 2 ) according to Carter (1975b). Haugh unit = 100 x log (AH x EW 0.37 ) where AH, albumin height and EW, egg weight. At 14 weeks of age, 15 blood samples (five samples of each category) were collected in heparinized tubes from wing vein of quail in the morning. Blood samples divided into two parts, the first part was used to determine the hematological parameters (RBCs, WBCs, PCV and Hb) and the second part was centrifuged at 3000 rpm for 15 minutes to separate plasma, and stored at -20 C until the time of determination. Plasma constituents were determined calorimetrically using kits according to Gornal et al. (1949) for TP, Dumas and Biggs (1971) for Alb, Gindler and King (1972) for Ca, El-Merzabani et al. (1977) for P, Zollner and Kirsch (1962) for TL, Fassati and Prencipe (1982) for TG, Allain et al. (1974) for cholesterol, Trinder (1969) for glucose and Reitman and Frankel (1957) for AST and ALT. Analysis data and correlation coefficients were computed according to SPSS (1999). Significant differences among means were evaluated using Duncan s multiple range test (Duncan, 1955). RESULTS AND DISCUSSION Average BW of light, medium and heavy weight groups of Japanese quail females at 6 weeks of age were , and g, respectively as shown in Table 1. The EW and CW increased significantly (P 0.01) with increasing the BW 6 and being 12.38, and and 8.50, 9.14 and 9.20 g, respectively. These results are in agreement with those reported by Yalçin et al. (1996); Ipek and Dikmen (2007); Cavero and Schmutz (2009); Alkan et al. (2010). However, insignificant differences were found in F% among the three groups (P>0.05) and the lowest value was % for the light group but the highest value was 92.56% for the medium group. Similarly, Ipek et al. (2003) observed that the breeder pairs that had the heaviest BW displayed higher fertility rates. Medium and heavy groups had higher H/TE % compared to the light group being and vs 78.32, respectively. Insignificant increase was shown in H/FE % with increasing the body (Table 1). Also, Coban et al. (2008) found that increasing the parent body weight of Japanese quail caused elevation of hatchability %, similarly, Liptói and Hidas (2006); Wolc and Olori (2009) reported that the dam that laid the egg was the main source of genetic variation in H/FE%, suggesting a huge impact of egg quality traits. Medium and heavy groups had significantly higher plasma Ca, TL and TG than the light group as shown in Table (2). Also, Nestor and Emmerson (1990) and Riczu et al. (2004) observed that BW was correlated with total bone density in laying hens and Elaroussi et al. (1994) reported that Ca is one of the key elements required for maintenance and egg production. Also, Klasing (1998) showed that most Ca in laying hens is used for shell formation, and it plays a role in fat and carbohydrate metabolism (Singh and Panda, 1996). Similarly, El-Full et al. (2006) found that plasma TL of Japanese quail females selected for high BW 6 was higher than females of the randombred control. Likewise, Hassan et al. (2008) observed that the BW 6 of Japanese quail positively correlated with each of TL and TG. 707

4 There were insignificant differences among the three females body weight groups in each of plasma TP and alb, P, cholesterol, glucose, AST and ALT (Table 2). However, highly significant increases were found in RBCs and WBCs with increasing the body weight of females Japanese quail as shown in Table (2). Also, El-Full et al. (2006) observed increasing in WBCs of females selected for high BW 6. The heavy females group had the highest significant value of Hb% compared to other groups, while insignificant increases were observed in PCV % with increasing body weight. Hassan et al. (2008) reported that PCV% increased in two lines selected to high growth rate and BW 6 compared to control this may be because the tissues of that lines must be supplied with high values of oxygen to support metabolism. Results presented in Table (3) showed that there were significant differences in ESA, SWUS, E WD, EL, Alb W, SW, and ST among the females groups, and the highest value was of heavy group compared to medium and light groups. Similar results were reported by Silversides et al. (2006) of chicken and by Alkan et al. (2010) of Japanese quail. This because BW was correlated with total bone density in laying hens (Nestor and Emmerson 1990; Riczu et al. 2004). There was significant decrease in Haugh unit of heavy female group compared to the other groups as shown in Table (3). However, no significant differences were found in SI, YW, Y%, Alb%, S% and YI among the groups. Alkan et al. (2010) found decrease in YI of egg high line body weight Japanese quail compared to low line. Phenotypic correlations 1- Phenotypic correlations among fertility %( F %), hatchability% for total and fertile eggs (H/TE% and H/FE %) and chick weight (CW) and each of body weight, plasma biochemical and hematological parameters of Japanese quail females There were significant positive phenotypic correlations between F % of egg quail and each of plasma Alb, Ca, TL and TG. Also, H/TE % correlated positively with each of BW 6, plasma Alb, Ca, TL, TG and RBCs count of Japanese quail females, but its correlations were negative and significant with plasma P, glucose and ALT as shown in Table (4). Keshavarz (1994) observed that high plasma P decreases calcium absorption from the gut and calcium from the bone. The H/FE % positively correlated with plasma TP, Ca, RBCs count, WBCs count, Hb % and PCV% and negatively correlated with P, glucose and ALT. High significant (P 0.01) positive phenotypic correlations were found between CW of quail and each of BW 6, plasma TP, Alb, Ca, TL, TG, AST, RBCs count, WBCs count, Hb % and PCV%. Also, Wilson (1991) reported that the BW is important after sexual maturity because of high positive correlations were found between BW and EW, EW and CW at hatching. However, CW was correlated positively with each of P, glucose and ALT. 2- Phenotypic correlations among fertility %(F%), hatchability% for total and fertile eggs (H/TE% and H/FE%) and chick weight (CW) and egg quality traits of Japanese quail females Positive phenotypic correlations were found between F % of egg and SWUS, Y% and S% and negatively with Alb%. There was significant positive phenotypic correlation between H/TE % and YD but it-correlated negatively and significantly with Alb % as shown in Table (5). However, H/FE % correlated positively with YD and negatively with YI. Cavero and Schmutz (2009) found positive correlation between the egg shell quality and hatchability, but there was antagonism between high Haugh units and good hatchability (Barnett et al., 2004 ; Cavero and Schmutz, 2009).The quality of the egg which defines the micro-environment of the developing embryo was mostly responsible for the successful hatching of the egg. This implies that egg quality was 708

5 important in hatchability and can make substantial contribution to the genetic improvement of hatchability (Bennewitz et al., 2007). High significant (P 0.01) positive phenotypic correlations were found between CW and each of EW, ESA, EL Alb W and SW. While, it correlated significantly and positively with SWUS, YW and ST. but it correlated negatively with Y% (Table 5). Also, Altinel et al. (1996) said that external and internal quality traits of the eggs are significant in the poultry breeding for their influence on chick s quality and growth. While, Flock et al. (2007) observed positive correlation of yolk proportion with hatchability and chick quality. Kul and Seker (2004) reported that positive phenotypic correlation was found between the EW and the SW or ST and the values were being 0.6 and 0.21 respectively. Also, Farooq et al. (2001) on egg Fayoumi hens that said the EW is indicator to shell quality. It can be concluded that the medium and heavy females had blood parameters and egg quality traits caused improving in each of F%, H/TE %, H/FE % and CW of the Japanese quail. Therefore, the selection for high body weight or for high some blood parameters may be cause good effects on that traits. Table (1): Body weight at 6 weeks (BW6), egg weight (EW), chick weight (CW), fertility %( F%) and hatchability % for total and fertile eggs (H/TE % and H/TE %) of Japanese quail females differ in body weight. Groups Body weight SE Sig Traits Light Medium Heavy BW6 (g) c b a 1.26 *** EW (g) c b a 0.14 ** C W (g) 8.50 b 9.14 a 9.20 a 0.13 ** F% NS H/TE % b a ab 2.54 * H/FE % NS Means having different superscripts within each effect in the same row are significantly different at P 0.05 SE: standard error, Sig: significance, NS: not significant, *: significant at P 0.05, **: significant at P 0.01, ***: significant at P 0.001, 1 EW = Eggs weight that incubated Table (2): Plasma biochemical and hematological parameters of Japanese quail females differ in body weight. Groups Body weight Traits Light Medium Heavy SE Sig Total protein, mg/dl NS Albumin, mg/dl NS Calcium, mg/dl b a a 1.84 * Phosphorus, mg/dl NS Total lipids, mg/dl 1220 b 1705 a 1579 a ** Triglycerides, mg/dl b a a ** Cholesterol, mg/dl NS Glucose, mg/dl NS AST, U/ml NS ALT, U/ml NS RBCs (x 10 6 /mm 3 ) 4.06 c 4.88 b 5.12 a 0.06 *** WBCs (x 10 3 /mm 3 ) 97.5 b b a 6.23 ** Hb% b b a 0.40 * PCV % NS Means having different superscripts within each effect in the same row are significantly different at P 0.05 SE: standard error, Sig: significance, NS: not significant, *: significant at P 0.05, **: significant at P 0.01, ***: significant at P

6 Table (3): The egg quality traits of Japanese quail females differ in body weight. Groups Body weight SE Traits Light Medium Heavy Sig Egg weight, g b b a 0.14 *** Surface area, cm b b a 0.19 *** SWUS, mg/cm b ab a 0.66 * Egg length, cm 3.28 b 3.31 b 3.36 a 0.01 ** Egg width, cm 2.65 ab 2.62 b 2.69 a 0.01 * Shape index % NS Yolk height, mm NS Yolk diameter, cm 2.59 b 2.61 b 2.70 a 0.03 * Yolk weight, g NS Yolk % NS Albumin weight, g 7.47 b 7.64 b 8.11 a 0.11 *** Albumin % NS Shell weight, g 1.10 b 1.15 b 1.22 a 0.02 *** Shell % NS Yolk index % NS Haugh unit a a b 0.78 * Shell thickness, mm b b a ** Means having different superscripts within each effect in the same row are significantly different at P 0.05 SE: standard error, Sig: significance, NS: not significant, *: significant at P 0.05, **: significant at P 0.01, ***: significant at P 0.001, 1 SWUS: Shell weight per unit surface area Table (4): Correlation coefficients among fertility % (F%), hatchability % for total and fertile eggs (H/TE% and H/FE %) and chick weight (CW) and each of body weight, plasma biochemical and hematological parameters of Japanese quail females, Items F% Sig H/TE% Sig H/FE % Sig CW Sig BW 6, g NS * NS *** Total protein, mg/dl NS NS * ** Albumin, mg/dl ** ** NS ** Calcium, mg/dl * ** * ** Phosphorus, mg/dl NS * * ** Total lipids, mg/dl ** ** NS ** Triglycerides, mg/dl ** ** NS ** Cholesterol, mg/dl NS NS NS NS Glucose, mg/dl NS * * ** AST, U/ml NS NS NS * ALT, U/ml NS ** * ** RBCs (x 10 6 /mm 3 ) NS ** * ** WBCs (x 10 3 /mm 3 ) NS NS ** ** Hb% NS NS * ** PCV % NS NS * ** Sig: significance, NS: not significant, *: significant at P 0.05, **: significant at P 0.01,***: significant at P 0.001, BW 6 : Body weight at 6 weeks of age, AST: aspartate aminotransferase, ALT: alanine aminotransferase, RBCs: red blood cells, WBCs: white blood cells, Hb: hemoglobin, PCV: packed cell volume 710

7 Table (5): Correlation coefficients between fertility% (F%), hatchability % for total and fertile eggs (H/TE % and H/FE %) and chick weight (CW) and egg quality traits of Japanese quail females. Items F% Sig H/TE % Sig H/FE % Sig CW Sig Egg weight, g NS NS NS *** ESA NS NS NS *** SWUS * NS NS * Egg length NS NS NS ** Egg width NS NS NS NS Shape index NS NS NS NS Yolk diameter NS * * NS Yolk height NS NS NS NS Yolk index NS NS * NS Yolk weight NS NS NS * Yolk % * NS NS * Albumen weight NS NS NS *** Albumen % ** * NS Shell weight NS NS NS *** Shell % ** NS NS NS Haugh unit NS NS NS NS Shell thickness NS NS NS * Sig: significance, NS: not significant, *: significant at P 0.05, **: significant at P 0.01, ***:significant at P 0.001, 1 ESA: Surface area of incubated eggs, 2 SWUS: Shell weight per unit surface area REFERENCES Alkan, S., K. Karabağ, A. Galic, T. Karsli, M. S. Balcioğlu (2010). Effects of selection for body weight and egg production on egg quality traits in Japanese quails (Coturnix coturnix japonica) of different lines and relationships between these traits. Kafkas Univ Vet Fak Derg.16: Allain, C.C., L. S. Poon, C. S. G. Chan, W. Richmond and P. C. Fu (1974). Enzymatic determination of total serum cholesterol. Clin. Chem. 20: Altinel, A., H. Gunes, T. Kirmizibayrak, S. G. Corekci and T. Bilal (1996). The studies on egg quality characteristics of Japanese quails. J. Fac. Vet. Univ Istanbul. 22: Bacon, W. L. (1994). The effect of heparin injection on laying turkey hen very-low density lipoprotein metabolism in sexually immature and laying turkey hens. Comp. Biochem. Physiol. 109A: Barnett, D. M., B. L. Kumpula, R. L. Petryk, N. A. Robinson, R A. Renema and F. E. Robinson (2004). Hatchability and early chick growth potential of broiler breeder eggs with hairline cracks. J. Appl. Poult. Res. 13: Bennewitz, J., O. Morgades, R. Preisinger, G Thaller. and E. Kalm (2007). Variance component and breeding value estimation for reproductive traits in laying hens using a Bayesian threshold model. Poult. Sci. 86: Boorman, K. N., J. G. Volynchook and C. G. Belyavin (1989). Egg Shell Formation and Quality. In: Recent Developments in Poultry Nutrition. Eds. D. J. A. Cole and W. Haresign. Butterworths, Kent, England. Brillard, J. P. (2003). Practical aspects of fertility in poultry. World's Poult. Sci. J. 59: Carter, T.C. (1968). The hen egg. A mathematical model with three parameters. Br. Poult. Sci. 9:

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10 تاثير وزن جسن الملخص العربي االم وبعض قياساث الذم وصفاث جودة البيضه على الخصوبت والفقس ووزن كتكوث السمان الياباوى حىان عبذاهلل حسه قسى إ راج انذ اخ كه ح انضساعح- خايعح انف و يصش ذى اسرخذاو عذد 153 أ ث ركش س ا اتا ع ذ ع ش 14 اسث ع ت سثح خ س ح 2 أ ث / 1 ركش نذساسح ذأث ش ص اندسى تعط ق اساخ انذو صفاخ خ دج انث ض نالو عه سثح انخص تح انفقس ي س تا نهث ط انكه ا ي س تا نث ط ان خصة ص انكرك خ.ذى ذقس ى انط س ان ثالثح يد عاخ )خف فح ير سطح ثق هح( ذثعا ن ص اندسى ع ذ ع ش 6 أسات ع كا د ان ر سطاخ نال از نهزك س عه انرشذ ة كا د ان رائح كانران ص ادج ص انث ضح ص انكرك خ يع ا يع ص ادج ص اندسى ع ذ ع ش 6 اسات ع. ان د عح ير سطح ان ص كا ن ا اعه سثح فقس ي س تا نهث ط انكه ان د عر ان ر سطح انثق هح كا ن ا أعه سثح كانس و د كه ح خه سش ذاخ ثالث ح تثالصيا انذو اعه عذد كشاخ دو ح شاء ت ضاء. كا ن د عح اال از انثق هح انق ى االعه لعذد كشاخ الدو ال ت ضاء سثح ان خه ت يساحح سطح انث ضح ص قششج انث ض / حذج يساحح سطح ط ل عشض انث ض ص انث ي انث ض ص قششج انث ض س ك قششج انث ض ت ا كا د ز ان د عح االقم ف حذج يقاس ح تان د عاخ االخش. خذ اسذثاط يع ي خة ت سثح انخص تح كال ي سة االنث ي انكانس و انذ انكه ح انده سش ذاخ انثالث ح تثالصيا انذو ص قششج انث ض / حذج يساحح سطح سثح انصفاس سثح انقششج سهث ا يع سثح انث ي انث ض. سثح فقس انث ط ان س ب نهث ط انكه اسذثطد ا دات ا يع كال ي ص اندسى ع ذ ع ش سرح اسات ع سة االنث ي انكانس و انذ انكه ح انده سش ذاخ انثالث ح تثالصيا انذو عذد كشاخ انذو انح شاء قطش صفاس انث ض كا االسذثاط سهث ا يع سثح انف سف س انده ك ص ا ض ى االال اي ذشا سف ش ض تثالصيا انذو سثح انث ي انث ض. خذ اسذثاط يع ي خة ت سثح فقس انث ط ان س ب نهث ط ان خصة كال ي سة انثش ذ انكانس و تثالصيا انذو عذد كشاخ انذو انح شاء انث ضاء سثح ان خه ت ان ك اخ انخه ح قطش صفاس انث ض كا االسذثاط سهث ا يع سثح انف سف س انده ك ص ا ض ى االال اي ذشا سف ش ض تثالصيا انذو دن م انصفاس. خذ اسذثاط يع ي خة ت ص انكرك خ كال ي ص اندسى ع ذ ع ش سرح اسات ع سة انثش ذ االنث ي انكانس و انذ انكه ح انده سش ذاخ انثالث ح ا ض ى االسثاسذاخ اي ذشا سف ش ض تثالصيا انذو عذد كشاخ انذو انح شاء انث ضاء سثح ان خه ت سثح ان ك اخ انخه ح ص انث ض يساحح سطح انث ض ط ل انث ض ص انث ي انث ض ص قششج انث ض ص قششج انث ض / حذج يساحح سطح ص انصفاس س ك قششج انث ض. ف ح كا االسذثاط سهث ا يع سثح انف سف س انده ك ص ا ض ى االال اي ذشا سف ش ض تثالصيا انذو سثح صفاس انث ض. سر رح ي رنك ا ا از انس ا ان اتا الير سطح ثق هح ان ص ن ا صفاخ دو خ دج ت ط ذؤد إن ذحس ف كال ي ج سة انخص تح سثح انفقس س اء ي س تا إن عذد انث ط انكه ا نعذد انث ط ان خصة كزنك ذحس ف ص انكرك خ ان اذح. نزنك اال رخاب ن ص اندسى ا نثعط يك اخ انذو ان شذفعح قذ سثة ذأث شاخ خ ذج عه انصفاخ انر ذى دساسر ا. 714