Detection of Neospora caninum in the blood of Korean native cattle and dairy cows using PCR
|
|
- Maryann Nichols
- 6 years ago
- Views:
Transcription
1 ª ª (28) 48ƒ 2 Korean J Vet Res(28) 48(2) : 191~195 w1$3wxü/fptqpsbdbojovn Á 1ûw w 2 ( : ) Detection of Neospora caninum in the blood of Korean native cattle and dairy cows using PCR Sang-Eun Lee 1,2, Jung-Youn Lee 1, * 1 Research Institute of Veterinary Medicine, Chungnam National University, Daejeon , Korea 2 Korea Centers for Disease Control and Prevention, Seoul , Korea (Accepted: April 3, 28) Abstract : This study was performed to detect Neospora caninum in blood of 61 Korean native cattle and 5 dairy cows in Chungnam province. All of them were healthy and did not show any clinical signs. DNA was isolated from blood samples and a 328 bp fragment was amplified by PCR using primer pair Np21 and Np6. The PCR positive rate was 14.8% in Korean native cattle and % in dairy cows. Cows with 15.6% were a little higher than bulls with 12.5% in gender. The detection rate of over 3-year-old Korean native cattle was 28.6% in age. The results demonstrate that N. caninum DNA can be detected in blood by PCR. PCR analysis in blood may be useful to annually screening test for N. caninum infection in clinically healthy cattle. Keywords : blood, Korean native cattle, Neospora caninum, PCR yš Neospora(N.) caninum w Apicomplexa Coccidia, Sarcocystidea w» j ¾,,,, š šš [8, 11-13, 15, 16, 2], s ùkü, w ƒ w ùkù [1]. N. caninum w» w xw z w, xÿw s y [1, 4, 1], ƒ ù x, PCR w N. caninum DNA w š [1, 14, 15, 21, 25]. N. caninum ƒ w wš, O'Handley [24] w x mw xü N. caninum wþš, Okeoma [26] xü N. caninum DNA ƒ š, w xü N. caninum DNA ƒ ƒwš šw. ù ü x PCR w N. caninum screening ƒ ƒ š, wz ü s w» w» w w w w PCR w x N. caninum w š w. *Corresponding author: Jung-Youn Lee Research Institute of Veterinary Medicine, Chungnam National University, Daejeon , Korea [Tel: , Fax: , visionalive@hanmail.net] 191
2 192 Á Table 1. The detection rate of Neospora caninum using PCR in Korean native cattle and dairy cows in Chungnam province Sex Age Cows Bulls <3 >3 Korean native cattle Dairy cow NE * NP PR (%) NE * NP PR (%) Total * NE: number of examined, NP: number of positive, PR: positive rate v %/" 1$3 œ w w 61 y 5 w x w z, DNA extraction kit(bioneer Co., Korea) w DNA w. N. caninum w tachyzoites DNA extraction kit(bioneer Co., Korea) w DNA w 28.5 ng/l(qubit Fluorometer; Invitrogen Co., USA). xü N. caninum DNA yw» w primer NP6 5'-CTCGCCAG TCAACCTACGTCTTCT-3' NP21+ 5'-CCCAGTGCGT CCAATCCTGTAAC-3' w [23, 25], PCR 94 o C 1 DNA wk z, 94 o C 1, 63 o C 1, 74 o C 3 4z w z 72 o C 1 g. s PCR 2% agarose gel»w z Ethidium bromide w UV transilluminator s w. œ w w 61 y 5 w PCR w xü N. caninum w. PCR w 15.6%(7/45), 12.5%(2/16) ùkü ƒ š, 3 w 7.5%(3/4), %(6/21) ùk ü. w s³ 14.8%(9/61). wr 5 ùkü(table 1). Fig. 1. Detection of Neospora caninum DNA in blood of Korean native cattle. The amplified 328 bp product was subjected to electrophoresis in 2% agarose gel. M: 1 bp maker; P: positive control; Lane 1: negative; Lanes 2-4: positive; Lane 5: negative; N: negative control. š x¾ s ùk ú w w xÿw, zxÿw x Á k w w ƒ yš [4, 1]. ü xÿw w N. caninum w k mw w üš [1-3, 5-7]. ù Dubey [1] w yw zxÿwù xÿw xw» w,», p ywšw ƒ, x PCR w N. caninum
3 w PCR w xü Neospora caninum 193 DNA wü yw w š šw [21, 27]. N. caninum PCR w» w w primerƒ [25, 26, 3], NP6 NP21+ primer N. caninum Nc5 r s j p primer š š [17, 19, 3]. NP6 NP21+ primer w 328 bp p s xü N. caninum wš yw. wr w, w xü N. caninum 14.8%(9/61) x [6] šw û w w 47.8%(11/23) ûš, y [7] šw ü w w 17.9%(145/868) w, ½ [18] šw û w w 8.9%(11/123). w w x N. caninum w N. caninum s, w y,. y [7] (19.6%)ƒ (17.3%) š šw, (15.6%)ƒ (12.5%) ù, y [7] ùƒ s³ 2ƒ š w %(6/21) N. caninum q. ù 3 w ƒ 3 2 ƒ w. wr N. caninum w š šù [6, 22, 28], ƒ, qƒ ù N. caninum» y y. N. caninum w w y w sw w q. w, ü N. caninum y w ƒ wš, O'Handley [24] Serrano-Martinez [29] N. caninum oocystes x z,» xl N. caninum š šw, Okeoma [25] Ferre [14] x xü N. caninum wš šw. ù x w» w, Okeoma [25] xü N. caninum,, š šw, N. caninum 9 š w, N. caninum. w sww ƒ w. w ¾ zw ù e ƒ» wù PCR ùkü z kjš, qj w w w ƒ w w, l ù y w w [4, 1]. w q w» w wz w w w w ƒ w. œ w y w 61 w 5 x N. caninum w» w NP21+ NP6 primer w PCR w. w x 14.8% N. caninum ùk ü, ùkü. (15.6%)ƒ (12.5%) ù kü, 3 w (28.6%) ùkü., xü N. caninum DNAƒ N. caninum š q, wz N. caninum» jw x w PCR w. šx 1., ½z, y, ûy,,, ½. Neospora w s xw. wwz 23, 43, ½, y, ½z, x«, y,, y,, y. Neospora w. wwz 1997, 37, ½z, y, x, y,. ½. Neospora caninum w. w wz 1998, 38,
4 194 Á 4., ½x,.»w. 3q. pp , ù, x«, ½z, y, y, y,,,,, ½. xÿw w ü Neospora caninum w x w. wwz 1998, 38, x, ½, ½, xz, ³,,. Neospora caninum w wƒ, w ƒwz 21, 24, y. w Neospora caninum w w. wwz 23, 43, Barr BC, Anderson ML, Woods LW, Dubey JP, Conrad PA. Neospora-like protozoal infections associated with abortion in goats. J Vet Diagn Invest , Cho JH, Chung WS, Song KJ, Na BK, Kang SW, Song CY, Kim TS. Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection. Korean J Parasitol 25, 43, Dubey JP. Review of Neospora caninum and neosporosis in animals. Korean J Parasitol 23, 41, Dubey JP, Hartley WJ, Lindsay DS, Topper MJ. Fatal congenital Neospora caninum infection in a lamb. J Parasitol 199, 76, Dubey JP, Lindsay DS, Adams DS, Gay JM, Baszler TV, Blagburn BL, Thulliez P. Serologic responses of cattle and other animals infected with Neospora caninum. Am J Vet Res 1996, 57, Dubey JP, Rigoulet J, Lagourette P, George C, Longeart L, LeNet JL. Fatal transplacental neosporosis in a deer (Cervus eldi siamensis). J Parasitol 1996, 82, Ferre I, Aduriz G, del-pozo I, Regidor-Cerrillo J, Atxaerandio R, Collantes-Fernández E, Hurtado A, Ugarte-Garagalza C, Ortega-Mora LM. Detection of Neospora caninum in the semen and blood of naturally infected bulls. Theriogenology 25, 63, Ferroglio E, Pasino M, Romano A, Grande D, Pregel P, Trisciuoglio A. Evidence of Neospora caninum DNA in wild rodents. Vet Parasitol 27, 148, Jenkins MC, Parker C, Hill D, Pinckney RD, Dyer R, Dubey JP. Neospora caninum detected in feral rodents. Vet Parasitol 27, 143, Kaufmann H, Yamage M, Roditi I, Dobbelaere D, Dubey JP, Holmdahl JMO, Trees A, Gottstein B. Discrimination of Neospora caninum from Toxoplasma gondii and other apicomplexan parasites by hybridization and PCR. Mol Cell Probes 1996, 1, Kim JH, Lee JK, Hwang EK, Kim DY. Prevalence of antibodies to Neospora caninum in Korean native beef cattle. J Vet Med Sci 22, 64, Liddell S, Jenkins MC, Dubey JP. A competitive PCR assay for quantitative detection of Neospora caninum. Int J Parasitol 1999, 29, Marsh AE, Barr BC, Madigan J, Lakritz J, Nordhausen R, Conrad PA. Neosporosis as a cause of equine protozoal myeloencephalitis. J Am Vet Med Assoc 1996, 29, McInnes LM, Ryan UM, O'Handley R, Sager H, Forshaw D, Palmer DG. Diagnostic significance of Neospora caninum DNA detected by PCR in cattle serum. Vet Parasitol 26, 142, Moore DP, Campero CM, Odeón AC, Posso MA, Cano D, Leunda MR, Basso W, Venturini MC, Späth E. Seroepidemiology of beef and dairy herds and fetal study of Neospora caninum in Argentina. Vet Parasitol 22, 17, Müller N, Zimmermann V, Hentrich B, Gottstein B. Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. J Clin Microbiol 1996, 34, O'Handley R, Liddell S, Parker C, Jenkins MC, Dubey JP. Experimental infection of sheep with Neospora caninum oocysts. J Parasitol 22, 88, Okeoma CM, Williamson NB, Pomroy WE, Stowell KM, Gillespie L. The use of PCR to detect Neospora caninum DNA in the blood of naturally infected cows. Vet Parasitol 24, 122, Okeoma CM, Stowell KM, Williamson NB, Pomroy WE. Neospora caninum: Quantification of DNA in the blood of naturally infected aborted and pregnant cows using real-time PCR. Exp Parasitol 25, 11, Ortega-Mora LM, Ferre I, del-pozo I, Caetano-da- Silva A, Collantes-Fernández E, Regidor-Cerrillo J, Ugarte-Garagalza C, Aduriz G. Detection of Neospora caninum in semen of bulls. Vet Parasitol 23, 117, Osawa T, Wastling J, Acosta L, Ortellado C, Ibarra J, Innes E. Seroprevalence of Neospora caninum infection in dairy and beef cattle in Paraguay. Vet Parasitol 22, 11,
5 w PCR w xü Neospora caninum Serrano-Martínez E, Ferre I, Martínez A, Osoro K, Mateos-Sanz A, Del-Pozo I, Aduriz G, Tamargo C, Hidalgo CO, Ortega-Mora LM. Experimental neosporosis in bulls: parasite detection in semen and blood and specific antibody and interferon-gamma responses. Theriogenology 27, 67, Yamage M, Flechtner O, Gottstein B. Neospora caninum: specific oligonucleotide primers for the detection of brain "cyst" DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). J Parasitol 1996, 82,
SYSTEMIC NEOSPOROSIS IN A WHITE RHINOCEROS
Journal of Zoo and Wildlife Medicine 41(1): 164 167, 2010 Copyright 2010 by American Association of Zoo Veterinarians SYSTEMIC NEOSPOROSIS IN A WHITE RHINOCEROS Angkana Sommanustweechai, D.V.M., Montakan
More informationHumoral immune response in pregnant heifers inoculated with Neospora caninum tachyzoites by conjunctival route
Veterinary Parasitology 148 (2007) 213 218 www.elsevier.com/locate/vetpar Humoral immune response in pregnant heifers inoculated with Neospora caninum tachyzoites by conjunctival route M.G. de Yaniz a,
More informationTRANSMISSION OF NEOSPORA CANINUM BETWEEN WILD AND DOMESTIC ANIMALS
J. Parasitol., 9(6), 24, pp. 6 65 American Society of Parasitologists 24 TRANSMISSION OF NEOSPORA CANINUM BETWEEN WILD AND DOMESTIC ANIMALS L. F. P. Gondim, M. M. McAllister, N. E. Mateus-Pinilla*, W.
More informationNeospora caninum. Neospora Caninum. tachyzoites
186-169 4 008 Neospora caninum 1 537 Neospora Caninum 6-4 anti- Bovine IgG tachyzoites tachyzoites %55 537/ 300 4 108 Rabbit %16 300/65 %4166 108 /45 1 169 Neospora caninum The Study of Neospora caninum
More informationOutline 1/13/15. Range is mostly surrounding Puerto Rico Important for Tourism and ecological balance
1/13/15 Prevalence of Toxoplasma gondii in Antillean manatees (Trichechus manatus manatus) and investigating transmission from feral cat feces in Puerto Rico Heidi Wyrosdick M.S. Candidate University of
More informationA survey of Neospora caninum-associated abortion in dairy cattle of Romania
A survey of Neospora caninum-associated abortion in dairy cattle of Romania Ovidiu Şuteu 1, Anamaria Paştiu 1, Adriana Györke 1, Gabriel Borza 1, Adrian Ardelean 2, Vasile Cozma 1 1 University of Agricultural
More informationJOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 2.417, ISSN: , Volume 4, Issue 2, March 2016
EPIDEMIOLOGY OF TOXOPLASMA GONDII INFECTION OF CATS IN SOUTHWEST OF ALBANIA SHEMSHO LAMAJ 1 GERTA DHAMO 2 ILIR DOVA 2 1 Regional Agricultural Directory of Gjirokastra 2 Faculty of Veterinary Medicine,
More informationApplication of a new therapeutic protocol against Neospora caninum-induced
1 Application of a new therapeutic protocol against Neospora caninum-induced abortion in cattle: a field study. Cuteri V. 1, Nisoli L. 2, Preziuso S. 1, Attili A.R. 1, Guerra C. 1, Lulla D. 2, Traldi G.
More informationNeosporosis in Sheep and Different Breeds of Goats from Southern Jordan: Prevalence and Risk Factors Analysis
American Journal of Animal and Veterinary Sciences 3 (2): 47-52, 2008 ISSN 1557-4555 2008 Science Publications Neosporosis in Sheep and Different Breeds of Goats from Southern Jordan: Prevalence and Risk
More informationSeroprevalence and risk factors of infections with Neospora caninum and Toxoplasma gondii in hunting dogs from Campania region, southern Italy
Institute of Parasitology, Biology Centre CAS doi: http://folia.paru.cas.cz Research Article Seroprevalence and risk factors of infections with Neospora caninum and Toxoplasma gondii in hunting dogs from
More informationSeroprevalence of Toxoplasma gondii in Sheep, Cattle and Horses in Urmia North-West of Iran
Tehran University of Medical Sciences Publication http:// tums.ac.ir Short Communication Iranian J Parasitol Open access Journal at http:// ijpa.tums.ac.ir Iranian Society of Parasitology http:// isp.tums.ac.ir
More informationA peer-reviewed version of this preprint was published in PeerJ on 18 January 2019.
A peer-reviewed version of this preprint was published in PeerJ on 18 January 2019. View the peer-reviewed version (peerj.com/articles/5920), which is the preferred citable publication unless you specifically
More informationProtozoan Parasites: Lecture 20 - Heteroxenous Coccidia - Part 1 Pages 39-51
Protozoan Parasites: Lecture 20 - Heteroxenous Coccidia - Part 1 Pages 39-51 Tissue cyst -forming Coccidia General Taxonomy Apicomplexa Heteroxenous Two host life cycles Asexual & sexual reproduction Intestinal
More informationPrevalence of antibodies against Neospora caninum in dogs from urban areas in Central Poland
Parasitol Res (2011) 108:991 996 DOI 10.1007/s00436-010-2143-0 ORIGINAL PAPER Prevalence of antibodies against Neospora caninum in dogs from urban areas in Central Poland Katarzyna Goździk & Robert Wrzesień
More informationOn the Biological and Genetic Diversity in Neospora caninum
Diversity 2010, 2, 411-438; doi:10.3390/d2030411 OPEN ACCESS diversity ISSN 1424-2818 www.mdpi.com/journal/diversity Review On the Biological and Genetic Diversity in Neospora caninum Sarwat E. Al-Qassab
More informationNeospora caninum and neosporosis
Neospora caninum and neosporosis Patrick S. Craig ASc, DVM, PhD (awarded) Neospora caninum is a protozoan cyst forming apicomplexan parasite that causes neosporosis, notably in cattle (Bos taurus) and
More informationSeroprevalence of Neospora caninum Infections of Dairy Cows in the North-east of Thailand
Kasetsart J. (Nat. Sci.) 42 : 61-66 (2008) Seroprevalence of Neospora caninum Infections of Dairy Cows in the North-east of Thailand Sathaporn Jittapalapong, 1 * Arkom Sangwaranond, 1 Tawin Inpankaew,
More informationProtozoan Parasites: Lecture 21 Apicomplexans 3 Heteroxenous Coccidia - Part 1 Pages 37-49
Protozoan Parasites: Lecture 21 Apicomplexans 3 Heteroxenous Coccidia - Part 1 Pages 37-49 Tissue cyst -forming Coccidia General Taxonomy Apicomplexa Heteroxenous Two host life cycles Asexual & sexual
More informationEpidemiology and Molecular Prevalence of Toxoplasma gondii in Cattle Slaughtered in Zahedan and Zabol Districts, South East of Iran
Iran J Parasitol: Vol. 13, No. 1, Jan-Mar 2018, pp.114-119 Iran J Parasitol Tehran University of Medical Sciences Publication http://tums.ac.ir Open access Journal at http://ijpa.tums.ac.ir Iranian Society
More informationPCR detection of Leptospira in. stray cat and
PCR detection of Leptospira in 1 Department of Pathology, School of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran 2 Department of Microbiology, School of Veterinary
More informationSEROLOGICAL SURVEY OF ANTIBODIES AGAINST TOXOPLASMA GONDII IN ORGANIC SHEEP AND GOAT FARMS IN GREECE
756 ISAH-2007 Tartu, Estonia SEROLOGICAL SURVEY OF ANTIBODIES AGAINST TOXOPLASMA GONDII IN ORGANIC SHEEP AND GOAT FARMS IN GREECE Ntafis, V. 1, Xylouri, E. 1, Diakou, A. 2, Sotirakoglou, K. 3, Kritikos,
More informationEpidemiological survey and pathological studies on Caprine arthritis-encephalitis (CAE) in Japan
Epidemiological survey and pathological studies on Caprine arthritis-encephalitis (CAE) in Japan Misako KONISHI 1), Makoto HARITANI 2), Kumiko KIMURA 2), Takamitsu TSUBOI 3), Hiroshi SENTSUI 4) & Kenji
More informationELISA assays for parasitic and tick-borne diseases
ELISA assays for parasitic and tick-borne diseases We are passionate about the health and well-being of humans and animals. Immunodiagnostics from contribute to a global, adequate supply of safe and nutritious
More informationSero-Prevalence of Toxoplasma Gondii in Different Horses Groups from Khartoum State, Sudan
Research Article 152 Sero-Prevalence of Toxoplasma Gondii in Different Horses Groups from Khartoum State, Sudan Abdalla Mohamed Ibrahim 1* ; Osman Mukhtar Osman 2 ; Rabab Haroun Mohamed Ali 1 ; Ahmed Ali
More informationPrevalence of Toxoplasma gondii antibodies, circulating antigens and DNA in stray cats in Shanghai, China
Wang et al. Parasites & Vectors 2012, 5:190 RESEARCH Open Access Prevalence of Toxoplasma gondii antibodies, circulating antigens and DNA in stray cats in Shanghai, China Quan Wang *, Wei Jiang, Yong-Jun
More informationSerological assays and PCR for detection of Toxoplasma gondii infection in an ostrich farm at Ismailia Provine, Egypt
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-issn: 2319-2380, p-issn: 2319-2372. Volume 2, Issue 3 (Jan. - Feb. 2013), PP 56-60 Serological assays and PCR for detection of Toxoplasma
More informationDetermination of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses
346 Praca oryginalna DOI: 10.21521/mw.5707 Med. Weter. 2017, 73 (6), 346-351 Original paper Determination of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses by duplex PCR, immunohistochemistry
More informationPrevalence of Bovine Leukemia Virus in Young, Purebred Beef Bulls for Sale in Kansas
Prevalence of Bovine Leukemia Virus in Young, Purebred Beef Bulls for Sale in Kansas David P. Gnad, DVM, MS, DABVP a Jan M. Sargeant, DVM, MS, PhD b Peter J. Chenoweth, DVM, PhD, DACT a Paul H. Walz, DVM,
More informationSeroprevalenceofToxoplasmaGondiiandNeosporaCaninumInfectioninCattleinGrenadaWestIndies
: G Veterinary Science and Veterinary Medicine Volume 14 Issue 2 Version 1.0 Year 2014 Type: Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online ISSN:
More informationInvestigation of bovine tuberculosis outbreaks by using a trace-back system and molecular typing in Korean Hanwoo beef cattle
Original Article J Vet Sci 2018, 19(1), 45-50 ㆍ https://doi.org/10.4142/jvs.2018.19.1.45 JVS Investigation of bovine tuberculosis outbreaks by using a trace-back system and molecular typing in Korean Hanwoo
More informationSession Pathology and Hygiene
PROCEEDINGS OF THE 11 th WORLD RABBIT CONGRESS Qingdao (China) - June 15-18, 2016 ISSN 2308-1910 Session Pathology and Hygiene Li Y., Wang Y., Tao G., Cui Y., Suo X., Liu X. PROPHYLACTIC AND THERAPEUTIC
More informationReproductive Vaccination- Deciphering the MLV impact on fertility
Reproductive Vaccination- Deciphering the MLV impact on fertility Safety Decision Efficacy Prebreeding Vaccination of Cattle should Provide fetal & abortive protection (BVD and BoHV-1) Not impede reproduction
More informationSegmental myelitis in cats caused by agents belonging
Case Report J Vet Intern Med 2011;25:148 152 Segmental Meningomyelitis in 2 Cats Caused by Toxoplasma gondii L. Alves, D. Gorgas, M. Vandevelde, G. Gandini, and D. Henke Segmental myelitis in cats caused
More informationCopyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and
Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere
More informationFor Public Health Personnel
For Public Health Personnel General Information Toxoplasma gondii is a protozoal parasite capable of infecting any warm-blooded animal, including humans. Wild and domestic cats are the only known definitive
More informationExperimental induction of the two-host life cycle of Sarcocystis cruzi between dogs and Korean native calves
227 The Korean Journal of Parasitology Vol. 39, No. 3, 227-232, September 2001 Experimental induction of the two-host life cycle of Sarcocystis cruzi between dogs and Korean native calves Sung-Hwan WEE
More informationAPPRAISAL OF THE EPIDEMIOLOGY OF NEOSPORA CANINUM INFECTION IN COSTA RICAN DAIRY CATTLE
APPRAISAL OF THE EPIDEMIOLOGY OF NEOSPORA CANINUM INFECTION IN COSTA RICAN DAIRY CATTLE Promotor Prof.dr.ir. M.C.M. de Jong Hoogleraar Kwantitatieve Veterinaire Epidemiologie Wageningen Universiteit Co-promotoren
More informationDermatitis in a dog associated with an unidentified Toxoplasma gondii-like parasite
Veterinary Parasitology 116 (2003) 51 59 Short communication Dermatitis in a dog associated with an unidentified Toxoplasma gondii-like parasite J.P. Dubey a,, A.L. Pimenta b, L.C.S. Abboud b, R.R. Ravasani
More informationAbove: life cycle of toxoplasma gondii. Below: transmission of this infection.
Toxoplasmosis PDF This article is based on a paid for research paper dated 1972 of similar title and authored by J.K.Frenkel and J.P. Dubey. It was published by The Journal of Infectious Diseases Vol.
More informationPARASITOLOGICAL EXAMINATIONS CATALOGUE OF SERVICES AND PRICE LIST
INSTITUTE OF PARASITOLOGY Biomedical Research Center Seltersberg Justus Liebig University Giessen Schubertstrasse 81 35392 Giessen Germany Office: +49 (0) 641 99 38461 Fax: +49 (0) 641 99 38469 Coprological
More informationSeroprevalence and risk factors of infections with Neospora caninum and Toxoplasma gondii in hunting dogs from Campania region, southern Italy
Institute of Parasitology, Biology Centre CAS Folia Parasitologica 2016, 63: 012 doi: 10.14411/fp.2016.012 http://folia.paru.cas.cz Research Article Seroprevalence and risk factors of infections with Neospora
More informationDetermining the Most Prevalent Parasitic Worms Found in Canines Surrounding the Bryan/College Station Area
Determining the Most Prevalent Parasitic Worms Found in Canines Surrounding the Bryan/College Station Area Yineli Carreon, Katie Freeman, Jesus Garcia, Cierra Briggs, Koren Dunn, Morgan De Shields, and
More informationEndogenous transplacental transmission of Neospora caninum during successive pregnancies across three generations of naturally infected sheep
https://doi.org/10.1186/s13567-018-0601-3 RESEARCH ARTICLE Open Access Endogenous transplacental transmission of Neospora caninum during successive pregnancies across three generations of naturally infected
More informationInternationalJournalofAgricultural
www.ijasvm.com IJASVM InternationalJournalofAgricultural SciencesandVeterinaryMedicine ISSN:2320-3730 Vol.5,No.1,February2017 E-Mail:editorijasvm@gmail.com oreditor@ijasvm.comm@gmail.com Int. J. Agric.Sc
More informationThe South American opossum, Didelphis marsupialis, from Brazil as another definitive host for Sarcocystis speeri Dubey and Lindsay, 1999
The South American opossum, Didelphis marsupialis, from Brazil as another definitive host for Sarcocystis speeri Dubey and Lindsay, 1999 589 J. P. DUBEY *, C. E. KERBER, D. S. LINDSAY, N. KASAI and H.
More informationSeroprevalence of Encephalitozoon cuniculi and Toxoplasma gondii in domestic rabbits (Oryctolagus cuniculus) in China
ISSN (Print) 0023-4001 ISSN (Online) 1738-0006 BRIEF COMMUNICATION Korean J Parasitol Vol. 53, No. 6: 759-763, December 2015 http://dx.doi.org/10.3347/kjp.2015.53.6.759 Seroprevalence of Encephalitozoon
More informationToxoplasma gondii CFT IHAT %81.3 %80.3 % %26.2 IFAT % %32.17 %40.86
% %. %. Toxoplasma, gondii 00 00 % 00 I g G 00 00 % %. %. %. %.0 % %. %. %. %. CFT, IHAT %. %0. %. CFT %. CFT CFT IFAT %. Toxoplasma gondii %. 0 %. 0 %0. CFT IHAT % IHAT CFT IHAT %.. %. IHAT %. %.0 %.
More informationSalmonella Dublin: Clinical Challenges and Control
Salmonella Dublin: Clinical Challenges and Control Simon Peek BVSc, MRCVS PhD, DACVIM, University of Wisconsin-Madison School of Veterinary Medicine Advancing animal and human health with science and compassion
More informationNeosporosis in a white rhinoceros (Ceratotherium simum) calf
Special report Spesiale verslag Neosporosis in a white rhinoceros (Ceratotherium simum) calf J H Williams a, I Espie b, E van Wilpe c and A Matthee b ABSTRACT A 16-day-old white rhinoceros calf died suddenly
More informationA Long-Term Study of Neospora caninum Infection in a Swedish Dairy Herd
Acta vet. scand. 2003, 44, 63-71. A Long-Term Study of Neospora caninum Infection in a Swedish Dairy Herd By Susanne Stenlund 1,3, Hans Kindahl 1, Arvid Uggla 2 and Camilla Björkman 3 1 Department of Obstetrics
More informationDiurnal variation in microfilaremia in cats experimentally infected with larvae of
Hayasaki et al., Page 1 Short Communication Diurnal variation in microfilaremia in cats experimentally infected with larvae of Dirofilaria immitis M. Hayasaki a,*, J. Okajima b, K.H. Song a, K. Shiramizu
More informationSeroprevalence and risk factors of Toxoplasma gondii in Tibetan Sheep in Gansu province, Northwestern China
Yin et al. BMC Veterinary Research (2015) 11:41 DOI 10.1186/s12917-015-0358-0 RESEARCH ARTICLE Open Access Seroprevalence and risk factors of Toxoplasma gondii in Tibetan Sheep in Gansu province, Northwestern
More informationThe detection of Cytauxzoon felis in apparently healthy free-roaming cats in the USA
Veterinary Parasitology 146 (2007) 316 320 www.elsevier.com/locate/vetpar The detection of Cytauxzoon felis in apparently healthy free-roaming cats in the USA Marion D. Haber a, Melissa D. Tucker a, Henry
More informationPCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea
ORIGINAL ARTICLE Korean J Parasitol. Vol. 48, No. 1: 9-13, March 2010 DOI: 10.3347/kjp.2010.48.1.9 PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea
More informationIsolation and biological and molecular characterization of Toxoplasma gondii from canine
JCM Accepts, published online ahead of print on 24 September 2014 J. Clin. Microbiol. doi:10.1128/jcm.02001-14 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 1 2 Isolation and
More informationIntroduction. Original Research 2 /13. Sarcocystis in wild birds of Mexico.
1 / 13 Veterinaria OA México Publicación Digital de la Facultad de Medicina Veterinaria y Zootecnia oa http://www.revistas.unam.mx/index.php/veterinaria-mexico Sarcocystis sp. parasites in the Mexican
More informationOIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : 2016-02-03 11:54:54 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Enzootic
More informationThe surveillance programme for bovine virus diarrhoea (BVD) in Norway 2016
Annual Report The surveillance programme for bovine virus diarrhoea (BVD) in Norway 2016 Norwegian Veterinary Institute The surveillance programme for bovine virus diarrhoea (BVD) in Norway 2016 Content
More informationSystemic Apicomplexans. Toxoplasma
Systemic Apicomplexans Toxoplasma Protozoan Groups Historically, protozoa have been grouped by mode of motility. Flagellates Hemoflagellates Trypanosoma cruzi Leishmania infantum Mucoflagellates Tritrichomonas
More informationReproductive ability of a cloned male detector dog and behavioral traits of its offspring
Original Article J Vet Sci 0, 7(), 07- ㆍ http://dx.doi.org/0./jvs.0.7..07 JVS Reproductive ability of a cloned male detector dog and behavioral traits of its offspring Ji Hyun Lee,, Geon A Kim,, Rak Seung
More informationOccurrence of antibodies to Neospora caninum and Toxoplasma gondii in dairy cattle from the northern region of the Paraná State, Brazil
Occurrence of antibodies to Neospora caninum and Toxoplasma gondii in dairy cattle from the northern region of the Paraná State, Brazil [Ocorrência de anticorpos contra Neospora caninum e Toxoplasma gondii
More informationInvestigations into novel pathogens associated with bovine reproductive failure
Investigations into novel pathogens associated with bovine reproductive failure Nick Wheelhouse Bovine Reproductive failure Economics Poor reproductive performance across a herd costs estimated 231 per
More informationSero-diagnosis of toxoplasmosis by using lateral flow chromatographic assay
International Journal of Natural and Social Sciences, 2018, 5(1): 25-29 ISSN: 2313-4461 Sero-diagnosis of toxoplasmosis by using lateral flow chromatographic assay Md. Billal Hossain 1 *, Md. Younus Ali
More informationThe First Report of Seropositivity for Neospora caninum in Sheep from Turkey
The First Report of for Neospora caninum in Sheep from Turkey, 1 Mor, N., 2 * Kırmızıgül, A.H., 1 Bozukluhan, K. 1 and Erkılıç, E.E. 1 1 Department of Internal Diseases, Faculty of Veterinary Medicine,
More informationTherapeutic efficacy of a mixture of ivermectin and closantel against gastrointestinal parasites in draft horses
( - ) ( ) % 88.0 19 %15.75 Oxyuris equi % 1.58 Strongylus spp..% 42.10 / 0.05.% 10.52 Parascaris equorum Parascaris equorum % 100 14 Strongylus spp. % 99.42 Oxyuris equi.gastrophilus nasalis Therapeutic
More informationDiseases of Concern: BVD and Trichomoniasis. Robert Mortimer, DVM Russell Daly, DVM Colorado State University South Dakota State University
Diseases of Concern: BVD and Trichomoniasis Robert Mortimer, DVM Russell Daly, DVM Colorado State University South Dakota State University The Epidemiologic Triad Host Management Agent Environment Trichomoniasis
More informationNA 100 R. Multi-functional electrophoresis device
NA 100 R Multi-functional electrophoresis device No need for UV transilluminator and darkroom You can see DNA bands after 2 or 3 minutes of electrophoresis You can check 80 PCR products at a time. No need
More informationSuggested vector-borne disease screening guidelines
Suggested vector-borne disease screening guidelines SNAP Dx Test Screen your dog every year with the SNAP Dx Test to detect exposure to pathogens that cause heartworm disease, ehrlichiosis, Lyme disease
More informationBiology 120 Lab Exam 2 Review
Biology 120 Lab Exam 2 Review Student Learning Services and Biology 120 Peer Mentors Sunday, November 26 th, 2017 4:00 pm Arts 263 Important note: This review was written by your Biology Peer Mentors (not
More informationCongenital Neosporosis in Goats from the State of Minas Gerais, Brazil
ISSN (Print) 0023-4001 ISSN (Online) 1738-0006 CASE REPORT Korean J Parasitol Vol. 50, No. 1: 63-67, March 2012 http://dx.doi.org/10.3347/kjp.2012.50.1.63 Congenital Neosporosis in Goats from the State
More informationToxoplasma gondii and Neospora caninum in farm-reared ostriches (Struthio camelus) in China
Feng et al. BMC Veterinary Research (2017) 13:301 DOI 10.118/s12917-017-1221-2 RESEARCH ARTICLE Open Access Toxoplasma gondii and Neospora caninum in farm-reared ostriches (Struthio camelus) in China Yongjie
More informationToxoplasma infection in sheep from south of Iran monitored by serological and molecular methods; risk assessment to meat consumers
Veterinary World, EISSN: 2231-0916 Available at www.veterinaryworld.org/vol.9/august-2016/9.pdf RESEARCH ARTICLE Open Access Toxoplasma infection in sheep from south of Iran monitored by serological and
More informationTOXOPLASMOSIS - AN OVERVIEW
TOXOPLASMOSIS - AN OVERVIEW I JP Dubey Zoonotic Diseases Laboratory, Livestock and Poultry Sciences Institute, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland 20705-2530,
More informationMolecular study for the sex identification in Japanese quails (Coturnix Japonica) Iran.
Molecular study for the sex identification in Japanese quails (Coturnix Japonica) Nasrollah Vali1 1 and Abbas Doosti 2 1 Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University,
More informationThe prevalence of anti-echinococcus antibodies in the North-Western part of Romania
The prevalence of anti-echinococcus antibodies in the North-Western part of Romania Anca Florea 1, Zoe Coroiu 2, Rodica Radu 2 1 Prof. dr. Octavian Fodor Regional Institute of Gastroenterology and Hepatology,
More informationUSDA KITTEN CANNIBALISM Cat and dog meat markets abroad. Taxpayer-funded animal testing at home.
USDA KITTEN CANNIBALISM Cat and dog meat markets abroad. Taxpayer-funded animal testing at home. A report by Jim Keen, DVM, PhD and White Coat Waste Project PO Box 26029 Washington, DC 20001 info@whitecoatwaste.org
More informationArchives of Razi Institute, Vol. 69, No. 2, December (2014) Razi Vaccine & Serum Research Institute
Archives of Razi Institute, Vol. 69, No. 2, December (2014) 165-170 Copyright 2014 by Razi Vaccine & Serum Research Institute Full Article Evaluation of Humoral Immune Response of Cats to the Experimental
More informationRICKETTSIA SPECIES AMONG TICKS IN AN AREA OF JAPAN ENDEMIC FOR JAPANESE SPOTTED FEVER
RICKETTSIA SPECIES AMONG TICKS IN AN AREA OF JAPAN ENDEMIC FOR JAPANESE SPOTTED FEVER Makoto Kondo 1, Katsuhiko Ando 2, Keiichi Yamanaka 1 and Hitoshi Mizutani 1 1 Department of Dermatology, 2 Department
More informationResearch Article Seroprevalence of Toxoplasma gondii in Dairy Cattle with Reproductive Problems in Sudan
ISRN Veterinary Science Volume 2013, Article ID 895165, 4 pages http://dx.doi.org/10.1155/2013/895165 Research Article Seroprevalence of Toxoplasma gondii in Dairy Cattle with Reproductive Problems in
More informationNEOSPORA CANINUM AND TOXOPLASMA GONDII ANTIBODY PREVALENCE IN ALASKA WILDLIFE
Journal of Wildlife Diseases, 46(2), 2010, pp. 348 355 # Wildlife Disease Association 2010 NEOSPORA CANINUM AND TOXOPLASMA GONDII ANTIBODY PREVALENCE IN ALASKA WILDLIFE Erica Stieve, 1 Kimberlee Beckmen,
More informationPREVALENCE OF NEOSPORA CANINUM AND TOXOPLASMA GONDII ANTIBODIES IN SERA FROM CAMELS (CAMELUS DROMEDARIUS) IN RIYADH PROVINCE, SAUDI ARABIA By
Journal of the Egyptian Society of Parasitology, Vol. 41, No. 2, August 2011 J. Egypt. Soc. Parasitol., 41 (2), 2011: 245 250 PREVALENCE OF NEOSPORA CANINUM AND TOXOPLASMA GONDII ANTIBODIES IN SERA FROM
More informationBLUETONGUE The Netherlands 2006
BLUETONGUE The Netherlands 06 Latitude: North 50 56 29 GD Deventer GD Deventer GD Deventer SCFCAH 28 August 06 Till: 27-08-06, 12:00 hrs 0 Agenda Infected area / holdings Laboratory results Lessons learned
More informationOIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : 2017-01-13 10:41:13 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Enzootic
More informationNorth Florida Research and Education Center, University of Florida, Marianna, FL 2
Administration of Recombinant Bovine Somatotropin Prior to Fixed-time Artificial Insemination and the Effects on Pregnancy Rates and Embryo Development in Beef Heifers N. Oosthuizen 1, P. L. P. Fontes
More informationOcorrências de anticorpos anti-toxoplasma gondii, Neospora spp. e Sarcocystis neurona em equinos e cães do município de Pauliceia, São Paulo, Brasil
277 PRELIMINARY NOTE Occurrences of antibodies against Toxoplasma gondii, Neospora spp., and Sarcocystis neurona in horses and dogs in the municipality of Pauliceia, São Paulo, Brazil Ocorrências de anticorpos
More informationRevaccination with a reduced dose of Brucella abortus strain 19 vaccine of breeding cows in the Pampas region of Argentina
Rev. sci. tech. Off. int. Epiz., 1987, 6 (4), 1063-1071. Revaccination with a reduced dose of Brucella abortus strain 19 vaccine of breeding cows in the Pampas region of Argentina A.C. ODEÓN *, C.M. CAMPERO
More informationInterpretation of results from milk samples tested for mastitis bacteria with Mastit 4 qpcr test from DNA Diagnostic
Mastit 4 Interpretation of results from milk samples tested for mastitis bacteria with Mastit 4 qpcr test from DNA Diagnostic The 40th ICAR Biennial Session Puerto Varas, Chile, 24-28 october 2016 Jorgen
More informationSeroprevalence of IgG and IgM antibodies and associated risk factors for toxoplasmosis in cats and dogs from subtropical arid parts of Pakistan
Tropical Biomedicine 31(4): 777 784 (2014) Seroprevalence of IgG and IgM antibodies and associated risk factors for toxoplasmosis in cats and dogs from subtropical arid parts of Pakistan Ahmad, N. 1*,
More informationMycoplasma ovis. What is it and why do we care? American Sheep Industry Convention San Antonio, TX February 1, 2018
Mycoplasma ovis What is it and why do we care? American Sheep Industry Convention San Antonio, TX February 1, 2018 M. A. Highland, DVM, PhD, Dipl. ACVP Veterinary Medical Officer-Researcher USDA-ARS-Animal
More informationEstimating Neospora caninum prevalence in wildlife populations using Bayesian inference
Estimating Neospora caninum prevalence in wildlife populations using Bayesian inference Karla Moreno-Torres 1, Barbara Wolfe 1,2, William Saville 1 & Rebecca Garabed 3 1 Department of Veterinary Preventive
More informationAnimals & Reptiles (PA) LD P KER CHIPS. *** Variations
Animals & Reptiles (PA) LD P KER CHIPS 1 PA-AB thru PA-CW PA-AB Beaver PA-AF Bear *** PA-AJ Dancing Bears Embossed / v:e PA-AP Buffalo Head PA-AS Buffalo Head PA-AV Old Tom *** PA-BC House Cat PA-BG House
More informationOriginal Article Infectious Causes of Bovine Abortion in Qazvin Province, Iran
Archives of Razi Institute, Vol. 72, No. 4 (2017) 225-230 Copyright 2017 by Razi Vaccine & Serum Research Institute Original Article Infectious Causes of Bovine Abortion in Qazvin Province, Iran Kaveh
More informationOIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : 2016-12-27 06:20:17 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis
More informationSEROPREVALENCE OF Toxoplasma gondii ANTIBODIES IN DOMESTIC SHORT-HAIRED CATS (Felis catus) IN A WILDLIFE FACILITY IN MANILA ABSTRACT INTRODUCTION
Philipp J Vet Anim Sci 2013, 39 (1): 99-106 99 SEROPREVALENCE OF Toxoplasma gondii ANTIBODIES IN DOMESTIC SHORT-HAIRED CATS (Felis catus) IN A WILDLIFE FACILITY IN MANILA Marco F. Reyes 1, Verman G. Guevara
More informationTECHNICAL DOCUMENT. Incorporating Rules for Cattle Health Schemes. Issued: July Final Version
TECHNICAL DOCUMENT Incorporating Rules for Cattle Health Schemes Issued: July 2014 Contents Page Introduction 3 4 The Diseases 5 10 The Rules of CHeCS 11 17 Bulk Milk Monitoring Programmes 18 Infectious
More informationRats born to Brucella abortus infected mothers become latent carriers of Brucella
Original Article Rats born to Brucella abortus infected mothers become latent carriers of Brucella Md. Ariful Islam 1, Mst. Minara Khatun 1 and Beyong-Kirl Baek 2 1 Department of Microbiology and Hygiene,
More informationReceived 7 December 1998/Returned for modification 5 April 1999/Accepted 22 June 1999
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, Sept. 1999, p. 760 764 Vol. 6, No. 5 1071-412X/99/$04.00 0 Copyright 1999, American Society for Microbiology. All Rights Reserved. Identification of an IS711
More informationOutbreak of Ovine Abortion by Toxoplasmosis in Southeastern Brazil
37 Case Report Outbreak of Ovine Abortion by Toxoplasmosis in Southeastern Brazil Michelle de Paula Gabardo 1, Juliana Saes Vilaça de Oliveira 1, Roselene Ecco 1, Roberto M. C. Guedes 1* 1 Department of
More information04/02/2013. Parasites and breeding dogs: These parasites we don t hear so much about. Main internal parasites found in breeding kennels
Parasites and breeding dogs: These parasites we don t hear so much about Main internal parasites found in breeding kennels Isospora sp. Giardia sp. Toxocara canis Something else? Breeders burden I m kind
More informationSchmallenberg Virus Infections in Ruminants
Schmallenberg Virus Infections in Ruminants F. J. Conraths, B. Hoffmann, D. Höper, M. Scheuch, R. Jungblut, M. Holsteg, H. Schirrmeier, M. Eschbaumer, K. Goller, K. Wernike, M. Fischer, A. Breithaupt,
More information