OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :44:07 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis (Brucella abortus, B. melitensis, B. suis) Address of laboratory: Woodham Lane,Addlestone Surrey, KT15 3NB UNITED KINGDOM Tel.: Fax: address: Adrian.Whatmore@apha.gsi.gov.uk Website: Name (including Title) of Head of Laboratory (Responsible Official): Chris Hadkiss, APHA, New Haw, Addlestone, Surrey, KT15 3NB, UK (Chief Executive) Name (including Title and Position) of OIE Reference Expert: Dr Adrian Whatmore Head, Bacterial Characterisation Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic Nationally Internationally c/i Milk RBT CFT Direct diagnostic Nationally Internationally Phenotypic Characterisation 32 6 Isolation from tissues 2642 RT PCR <10 <10 Bruceladder <20 <20 SNP 20 8 MLST MLVA <10 <10 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 2 OIE Reference Laboratory Reports Activities, 2016
3 NOTE: Currently, there are 22 laboratories that produce Standard Reference Reagents officially recognised by the OIE for 19 diseases/pathogens. Please click the following link to the list of OIE-approved International Standard Sera: If the reagent is not listed on this page, it is NOT considered OIE-approved. The next two questions allow you to indicate non-oie-approved diagnostic reagents. Disease Test Available from Bovine brucellosis Brucella abortus Ovine and caprine brucellosis (anti- Brucella melitensis [ISaBmS]) Indirect and Competitive enzyme-linked immunosorbent assay; Complement fixation; Agglutination; Buffered Brucella antigen ; Fluorescence polarisation assay Complement fixation; Agglutination; Buffered Brucella antigen ; Fluorescence polarisation assay; Indirect and Competitive enzyme-linked immunosorbent assay Indirect and Competitive enzyme-linked immunosorbent assay; Fluorescence polarisation assay; Buffered Brucella antigen Dr Adrian Whatmore Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom Tel: ( ) Fax: ( ) adrian.whatmore@apha.gsi.gov.uk Dr Adrian Whatmore (as above) Dr Adrian Whatmore (as above) OIE Reference Laboratory Reports Activities,
4 Type of reagent available Related diagnostic test Produced/ imported Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Name of recipient OIE Member Countries DENMARK FINLAND HONDURAS GERMANY MOLDOVA COLOMBIA BELGIUM UNITED STATES OF AMERICA ISaBMS DENMARK ISaBMS BELGIUM 4 OIE Reference Laboratory Reports Activities, 2016
5 Standards INDIA Standards LATVIA Standards KOREA (DEM. PEOPLE'S. REP. OF) Standards COLOMBIA Standards UNITED STATES OF AMERICA RBT Antigen RBT MRT Antigen MRT SAT Antigen SAT CFT Antigen CFT Monospecific Sera Phenotyic characteristaion 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 4. Did your laboratory produce vaccines? OIE Reference Laboratory Reports Activities,
6 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses TANZANIA December 0 5 GAMBIA vember 2 UNITED ARAB EMIRATES May 40 ARGENTINA December 5 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? 6 OIE Reference Laboratory Reports Activities, 2016
7 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided TAJIKISTAN Advice on laboratory diagnostics In country/electronic LIBYA Advice - paper Electronic UNITED ARAB EMIRATES Surveillance advice and testing Electronic NORWAY Advice - serology Electronic AZERBAIJAN Advice - vaccines Electronic ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,
8 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Establishment of a multisectoral strategy for the control of brucellosis in the main periurban dairy production zones of West and Central Africa 5 years Capacity building to aid control of brucellosis in the main periurban dairy production zones of West and Central Africa SENEGAL Molecular epidemiology of brucellosis in northern Tanzania 3 years Understanding brucellosis in Tanzania with the aim of providing the evidence base to inform control policy TANZANIA Combined use of novel diagnostic tools and strategic vaccination to control bovine brucellosis in endemic areas. 3 years INDIA Development of a multiplexed diagnostic biosensor for infectious reproductive diseases of cattle and buffaloes. 3 years INDIA Epidemiology of brucellosis in Tajikistan. 3 years Capacity building to improve brucellosis diagnostic capabilities in human and veterinary sectors. TAJIKISTAN 8 OIE Reference Laboratory Reports Activities, 2016
9 ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 Ayoola MC, Ogugua AJ, Akinseye VO, Joshua TO, Banuso MF, Adedoyin FJ, Adesokan HK, Omobowale TO, Abiola JO, Otuh PI, ttidge HO, Dale EJ, Perrett L, Taylor A, Stack J, Cadmus SI. Sero-epidemiological survey and risk factors associated with brucellosis in dogs in south-western Nigeria. Pan Afr Med J Feb 4;23:29. Whatmore AM, Koylass MS, Muchowski J, Edwards-Smallbone J, Gopaul KK, Perrett LL. Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the Genus Brucella: Phylogeography and Relationship to Biovars. Front Microbiol Dec 21;7:2049. Scholz HC, Mühldorfer K, Shilton C, Benedict S, Whatmore AM, Blom J, Eisenberg T. The Change of a Medically Important Genus: Worldwide Occurrence of Genetically Diverse vel Brucella Species in Exotic Frogs. PLoS One Dec 30;11(12):e Scholz HC, Revilla-Fernández S, Al Dahouk S, Hammerl JA, Zygmunt MS, Cloeckaert A, Koylass M, Whatmore AM, Blom J, Vergnaud G, Witte A, Aistleitner K, Hofer E. Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes). Int J Syst Evol Microbiol May;66(5): Mahmood R, Waheed U, Ali T, Gopaul KK, Dainty AC, Muchowski JK, Koylass MS, BREW SD, Perrett LL; Whatmore AM, Khan QM. Serological and nucleic acid based detection of brucellosis in livestock species and molecular characterization of Brucella melitensis strains isolated from Pakistan. International Journal of Agriculture and Biology (2): b) International conferences: 2 Brucellosis International Research Conference 2016, New Delhi, India European Association of Veterinary Laboratory Diagnosticians, Prague c) National conferences: 0 d) Other: (Provide website address or link to appropriate information) 1 Brucella MLST website/database ( OIE Reference Laboratory Reports Activities,
10 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 1 b) Seminars: 0 c) Hands-on training courses: 1 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Tajikistan 15 d Tanzania 1 c Tanzania 5 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO/IEC 17025/2005 ISO Certificate scan (PDF, JPG, PNG format) APHA Cert.pdf 9001 Certificate pdf 16. Is your laboratory accredited by an international accreditation body? 10 OIE Reference Laboratory Reports Activities, 2016
11 Test for which your laboratory is accredited RBT SAT CFT c i Milk Phenotypic Characterisation Evaluation of media Accreditation body 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency? OIE Reference Laboratory Reports Activities,
12 Purpose of the proficiency : 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Brucellosis Serum Testing Participant EU members ANSES France 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: of diagnostic reagents to countries not able to list in TOR2 (too numerous) Austria Argentina Afghanistan China Italy Spain France 12 OIE Reference Laboratory Reports Activities, 2016
13 Singapore South Africa Netherlands Taiwan New Zealand Roumania Sri Lanka Hungary rway Indonesia Nigeria Kenya Portugal Honduras OIE Reference Laboratory Reports Activities,
OIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2017 This report has been submitted : 2018-01-17 11:35:03 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis
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