OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2016 This report h been submitted : :55:37 Name of disee (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis (Brucella abortus, B. melitensis) Address of laboratory: National Institute of Animal Health, Department of Livestock Development 50/2 Ketklang Ladyao Chatuchak Bangkok THAILAND Tel.: to 14 Fax: / 19 address: monayae@dld.go.th Website: niah.dld.go.th Name (including Title) of Head of Laboratory (Responsible Official): Dr. Preecha Wongwicharn, Director of the National Institute of Animal Health, Dr. Reka Kanitpun, Chief of Immunology Section (Brucellosis Laboratory) Name (including Title Position) of OIE Reference Expert: Monaya Ekgatat,DVM, BSc., DMM. Brucellosis Advisor to DLD, National Institute of Animal Health, Department of Livestock Development Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote disseminate diagnostic methods validated according to OIE Stards 1. Did your laboratory perform diagnostic tests for the specified disee/topic for purposes such disee diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Test Indicated in OIE Manual (/) Total number of test performed lt year Indirect diagnostic tests Nationally Internationally RBT 63, CFT I-ELISA(bovine serum) I-ELISA(bovine milk) 1, I-ELISA(caprine/ovine serum 753 FPA 419 Milk Ring Test 120 B. canis RSAT/LFIA 121/3 Direct diagnostic tests Nationally Internationally Culture(organ/milk) 43/67 3/129 Brucella identification (animal) Brucella spp.pcr/real time PCR(specimens) 15/40 3 Brucella molecular typing Control of diagnostic antigen batches 7 ToR 2: To develop reference material in accordance with OIE requirements, 2 OIE Reference Laboratory Reports Activities, 2016
3 implement promote the application of OIE Stards. To store distribute to national laboratories biological reference products any other reagents used in the diagnosis control of the designated pathogens or disee. 2. Did your laboratory produce or supply imported stard reference reagents officially recognised by the OIE? 3. Did your laboratory supply stard reference reagents (non OIE-approved) /or other diagnostic reagents to OIE Member Countries? OIE Reference Laboratory Reports Activities,
4 Type of reagent available Related diagnostic test / provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients National stard panel of positive sera reagents batch control 160 vials(160x1ml) 15 vials(15x1ml) 3 National stard panel of negative sera reagents batch cintrol 80 vials(80x1ml) 15 vials(15x1ml) 3 Negative serum (for reconstitute) reagents batch control 3 bottles(3x5 ml) 9 bottles(9x5 ml) 3 National RBT stard serum (Bru Pos NIAH 05/2011 (Twinning Programme) RBT antigen batch control 12 vials(12x1ml) 15 vials(15x1ml) 3 National CFT stard serum (Bru Pos NIAH 04/2011 (Twinning Programme) CFT antigen batch control 15 vials(15x1ml) 3 4 OIE Reference Laboratory Reports Activities, 2016
5 RBT antigen reagent Provide 1 botlle(1x10ml) 1(training) MRT antigen reagent 3,000 tests RSAT antigen reagent 1,600 tests PCR(Bruce-ladder) PCR 200 tests 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, stardise validate, according to OIE Stards, new procedures for diagnosis control of the designated pathogens or disees 6. Did your laboratory develop new diagnostic methods validated according to OIE Stards for the designated pathogen or disee? OIE Reference Laboratory Reports Activities,
6 7. Did your laboratory develop new vaccines according to OIE Stards for the designated pathogen or disee? ToR 4: To provide diagnostic testing facilities,, where appropriate, scientific technical advice on disee control meures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking sistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses BHUTAN 23 March Serum 50 samples, milk 33 samples, organs 3 tubes(7organs) - BHUTAN 1 June Milk 54 samples BHUTAN 1 August Milk 42 samples 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice w provided PHILIPPINES Inter-laboratory Proficiency test: trouble shooting Remote sistance through communication MONGOLIA Inter-laboratory Proficiency test: trouble shooting Remote sistance through communication SRI LANKA Inter-laboratory Proficiency test: trouble shooting Remote sistance through communication ToR 5: To carry out /or coordinate scientific technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 6 OIE Reference Laboratory Reports Activities, 2016
7 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Development of Milk-ELISA kit for detection of antibody against Brucella spp. 2014/6-2017/03 Comparison of antibody titer between milk serum of cattle by using the Brucella ELISA kit which h been approved in Japan for diagnosis Optimization of preparation milk sample for diagnosis National institute of Animal Health (NIAH), JAPAN JAPAN ToR 6: To collect, process, analyse, publish disseminate epizootiological data relevant to the designated pathogens or disees 11. Did your Laboratory collect epizootiological data relevant to international disee control? 12. Did your laboratory disseminate epizootiological data that had been processed analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 1 1. Detection of Brucella melitensis Using Real-time PCR Isolation from Organs of Meat Goat in Thail Journal of Mahanakorn veterinary Medicine, Available online: b) International conferences: 5 1. Ekgatat M. Kanitpun R, 2016: Brucellosis: Current situation in Thail. International Conference on Veterinary Science 2016, Impact Forum Muang Thong Thani, Thail, vember 2016 (Invited speaker). 2. Conference on: Field Epidemiology Investigation of Zoonotic Animal Disee Under Field Epidemiology Training Program for Veterinarian (FETPV), June 2-3, 2016, National Institute of Animal Health, Bangkok. (Advisor commentator) 3. The Fourth Conference on Animal Health Situation/Surveillance Data Analysis, 2016 Under FETPV, October 31-vember 2, 2016, Amari Watergate hotel, Bangkok. (Advisor commentator) 4. FETPV Conference on Animal Outbreak Investigation (Research Presentation), October 14, 2016 October 21, (Advisor commentator) 5. Brucellosis New Delhi 2016: International Research Conference, National Agricultural Science Center (NASC), New Delhi, India, vember 17-19, (Participant) c) National conferences: 0 d) Other: (Provide website address or link to appropriate information) 4 1. FETPV - 1st Module 2016: Bic Epidemiology Surveillance Data Analysis (Proposal Presentation) June 10, 2016 July 15, 2016 (Advisor) OIE Reference Laboratory Reports Activities,
8 2. FETPV- 2nd Module 2016: Animal Health Situation Analysis (Research Presentation), October 26-28, (Advisor) 3. FETPV- Outbreak Demonstration Brucellosis in goat in Suphanburi province, vember 3-12, 2016, Suphanburi province. (Advisor) 4. Participation a working committee of Thai Agricultural Stard for Test of Brucellosis, National Bureau of Agricultural Commodity Food Stards, Ministry of Agriculture Cooperatives. ToR 7: To provide scientific technical training for personnel from OIE Member Countries To recommend the prescribed alternative tests or vaccines OIE Stards 14. Did your laboratory provide scientific technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 5 b) Seminars: 0 c) Hs-on training courses: 2 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Bhutan 1 a Japan 3 a Japan 2 a Japan 1 a Japan 2 c Mongolia 4 c Philippines, Malaysia, Vietnam (USDA, Animal Plant Health Inspection Service, International Services(APHIS IS) The Philippines Department of Agriculture, Bureau of Animal Industry)(April 18-22, 2016, Quezon City, Philippines) 16 ToR 8: To maintain a system of quality surance, biosafety biosecurity relevant for the pathogen the disee concerned 15. Does your laboratory have a Quality Management System certified according to an International Stard? 8 OIE Reference Laboratory Reports Activities, 2016
9 Quality management system adopted ISO 17025:2005 ISO 9001:2008 Certificate scan (PDF, JPG, PNG format) Cer. ISO 17025_2005 NIAH.pdf ISO9001_cert_NIAH_Thail[1].jpg 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Accreditation body RBT CFT ILAC-MRA by Bureau of Laboratory Quality Stard, Department of Medical Science ILAC-MRA by Bureau of Laboratory Quality Stard, Department of Medical Science 17. Does your laboratory maintain a biorisk management system for the pathogen the disee concerned? (See Manual of Tests Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish maintain a network with other OIE Reference Laboratories designated for the same pathogen or disee organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disee? 21. W your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the OIE Reference Laboratory Reports Activities,
10 same pathogen or disee by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. EU Bovine Brucellosis Serum Proficiency Test 2016 participant EU members Organizing OIE Ref. Lab.: ANSES, France - Bovine Brucellosis ILPT 2016 organizer 37 (National Regional Brucellosis Laboratories in - Brucellosis Laboratories within Thail) 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disee on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories The molecular epidemiological analysis of Brucella species from a variety of animals application on diagnostics for Brucella canis Identifying Brucellosis in Thail epidemiological correlation of Brucella isolates between two countries application evaluation on serological diagnostics of Brucella canis Animal Plant Quarantine Agency (QIA), Republic of Korea ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens disees to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disee? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point OIE Reference Laboratory Reports Activities, 2016
11 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Laboratory capability to serological tests on RBT, CFT I-ELISA (organizer) 37 (National Regional Brucellosis Laboratories in - Brucellosis Laboratories within Thail) ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: On question 10, answer, the table w pop-up with 5 columns. The 5th column w the list of countries for selection. It showed the selected country, but when submission, it did not shown in the text. OIE Reference Laboratory Reports Activities,
OIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : 2016-12-27 06:20:17 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis
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