HYMENOLEPIS NANA FROM RATTUS RATTUS OF ROHILKHAND WITH A NOTE ON THEIR HAZARDS TO HUMANS

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1 Proc.Zool.Soc.India. 16 (1) : : (2017) ISSN : INDEXED AND ABSTRACTED 11 HYMENOLEPIS NANA FROM RATTUS RATTUS OF ROHILKHAND WITH A NOTE ON THEIR HAZARDS TO HUMANS 1 1 D.K. GUPTA, NEELIMA GUPTA*2, SUDHIR KUMAR 1 Department of Zoology, Bareilly College, Bareilly India 2 Centre of Excellence Laboratory, Department of Animal Science, MJP Rohilkhand University, Bareilly India Received Accepted ABSTRACT Rattus rattus (n=65) were autopsied and their intestine examined for the presence of parasites. The intestine of 4 rats revealed whitish cestodes frequently attached to the intestinal wall and were identified as Hymenolepis nana (the dwarf tapeworm (length 22 to 43 mm; width mm). Scolex anterior, small, globular, (diameter 0.31 mm), cup-like, tetrad (four suckers) and retractile rostellum (row of hooks). Proglottid contains a single set of reproductive organs, genital pores unilateral. Testes three, ovary sub median, lobed ( mm by mm). Gravid proglottids (length mm, width mm) filled with numerous eggs. Vitellaria compact and small. Eggs slightly oval (average X micrometers). Key words : Wild rat, cestode, Hymenolepis, dwarf tapeworm, rodent INTRODUCTION Hymenolepis nana (Cestoda: Taeniidae) is commonly known as the dwarf tapeworm and in the human body, it is the most frequently found cestode parasite. Infections are more common among children. It is most widespread in warm climates and around unsanitary areas where eggs can be passed through aecal matter from an infected host to an uninfected person. Hymenolepis nana can be found wherever humans and rodents live. They have been found in almost all types of terrestrial biomes. (Roberts and Janovy Jr., 2000). It lodges itself in the intestines and absorbs nutrients from the intestinal lumen (Cameron, 1956). Gupta et al. (1981) reported the effect of mode of infection on the development and chemotherapeutic response of H. nana in rats. In another report, the parasite occurred at an incidence of 9.9% in urban slum dwellers of India being highest amongst intestinal infections (Mirdha and Samantray, 2002). Goswami et al. (2011) recorded Hymenolepis diminuta from albino wistar rats and wild brown rats. Gupta et al. (2013) observed the serum chemistry in pregnant and non-pregnant rats, infected with cestode parasite and the condition factor and organosomatic (hepato and splenosomatic) indices of Rattus rattus parasitized by cestodes were also reported (Gupta et al., 2016). Kumar et al. (2016) recorded changes in haemoglobin in wild rats, infected with Hymenolepis diminuta. The present investigations were undertaken to characterize the morphological features and taxometry of the cestode parasite based on the morphology of the scolex, segments and egg structures. MATERIALS AND METHODS COLLECTION AND MAINTENANCE OF HOSTS R. rattus ( g) were trapped alive in rat traps from storehouses and godowns of different areas of Bareilly, U.P. India and nearby places. Trapped rats were immediately transported to the laboratory, maintained in rat cages and fed on food grains, bread crumbs and water ad libitum. To avoid the effect of stress, rats were dissected and examined preferably within 24 hours from capture. They were anaesthetized humanely using chloroform anesthesia and weight, length and sexes were recorded before dissecting the animal. (55)

2 GUPTA ET AL. FAECAL EXAMINATION The faecal samples were routinely examined macroscopically for the presence of cestode segments and microscopically by direct smear method for the presence of eggs. ISOLATION AND PROCESSING OF CESTODES Cestodes were found attached to the wall of the lumen with their suckers; therefore they were detached carefully with the help of a soft brush so that the scolex was not damaged. They were relaxed in normal saline. Often worms released eggs which was advantageous for cestode study as an egg lled uterus can hide the important reproductive structures which are essential for identi cation. The cestodes were washed with distilled water several times and xed in 10% formalin or Carnoy's uid. Correctly xed and attened worms were stored in 70% alcohol in labeled glass vials. The parasites were stained with routine techniques (Gupta et al., 2016) in Borax carmine and eosin, dehydrated, cleared and mounted in canada balsam. Photographs were taken under Olympus BX-53 microscope and the parasites measured with Cellsens software imaging system. RESULTS Hymenolepis nana (synonyms Dwarf tapeworm, Rodentolepis (Hymenolepis) microstoma, Rodentolepsiasis, Vampirolepis nana, Hymenolepis diminuta, Mathevotaenia, Rat tapeworm) TAXOMETRY Phylum - Platyhelminthes Class Cestoda Subclass Eucestoda Order - Cyclophyllidea Family - Hymenolepididae Genus Hymenolepis, Rodentolepis Species: nana PARASITE PROFILE Host Rattus rattus Locality Bareilly (U.P.) Prevalence 4/65 Mean intensity 1-3 parasites/host POST MORTEM EXAMINATION The faecal samples examined revealed the presence of cestode eggs bearing hooks. Such rats were further autopsied to investigate the presence of adult cestodes. The intestine of 4 out of 65 autopsied rats revealed whitish cestodes frequently attached to the intestinal wall. DESCRIPTION ADULT (Fig. 1) The worms were delicate, whitish and opaque when alive and the body is composed of proglottids (Fig. 1A). The adults of the dwarf tapeworm are 22 to 43 mm in length and mm in width. Scolex is small, 0.31 mm in diameter, almost globular (rounded), cup-like, situated at the anterior end of body, tetrad having four suckers and retractile rostellum with a row of hooks Fig. 1B,C). Scolex was followed by a narrow slender neck with segments wider than long (Fig. 1D). The genital pores are unilateral, situated on the side of the segment. Each segment contains a single proglottid, with a single set of reproductive organs. Each proglottid has both male and female reproductive organs, thus Hymenolepis nana is hermaphroditic (Fig. 1E). Each mature segment contains three testes, oval to elongated in shape. In the posterior mature proglottids, cirrus was also observed. Ovary sub median, (56)

3 PROC. ZOOL. SOC. INDIA towards the aporal side, and lobed measuring mm by mm. Vagina thin walled and tubular, occasionally hardly visible. Gravid (mature, full of eggs) proglottids are mm long and mm wide. The gravid proglottid is lled with numerous eggs, and the uterus is not visible (Fig. 1F). Vitellaria compact and small. Proglottids were never present in the faeces and therefore presumably disintegrate in the gastrointestinal tract. EGGS (Fig. 2) (measurements in µm) The eggs of H. nana are slightly oval at about (average 53.71) X (average 42.29) micrometers (Fig. 2A). The eggs of H. nana did not have a striated appearance which is unlike other taeniid eggs ( Lapage, 1951; Roberts and Janovy Jr., 2000; Ghaffar, 2001). Eggs were freely observed in the infected faecal samples, they are embryonated when laid containing a well developed 6-hooked onchosphere [30.13 x 26.33] which was (longitudinal axis) x 8.11 (transverse axis) away from the membrane (Fig. 2B,C). The hooks ranged from in length. The oncosphere is covered with a thin, hyaline, outer membrane and an inner, thick membrane with polar thickenings, "knobs" or poles that bear four laments and spread out between the two membranes lying between the inner and outer membranes and this was similar to those reported by Ghaffar (2001). A few eggs were observed in the rupturing stage releasing the oncosphere (Fig. 2D). The heavy embryophores that give taeniid eggs their characteristic striated appearance are lacking in this and the other families of tapeworms infecting humans. The rostellum remains invaginated in the apex of the organ. Rostellar hooklets are shaped like tuning forks. REMARKS Many species of the genus Hymenolepis, Weinland, 1858 are known from rodents of different countries (Southwell,1930; Hughes, 1940; Lapage,1956; Yamaguti, 1959; Smyth,1962) The present specimens were identi ed as Hymenolepis nana because they possessed characters similar to this parasite, such as rostellum with a row of 20 hooks, neck small, mature and gravid, segments similar to H. nana. DIAGNOSIS Diagnosis was based on nding eggs in the faecal samples. LIFE CYCLE The life cycle of the parasite was investigated earlier by Shorb (1933), Hunninen (1935), Kumazawa and Suzuki (1983) and Henderson and Hanna (1987), the latter authors provided valuable protocols for the maintenance of life cycle. Adult development from cysticercoids Rats 5-6 weeks old are suitable hosts. The cestodes establish themselves initially in the anterior small intestine but subsequently migrate to the lower ileum, where they remain for the duration of infection (Henderson and Hanna, 1987). Adult development from eggs In a direct egg infection, eggs hatch in the duodenum and oncospheres immediately penetrate the villi in which they develop into fully formed cysticercoids within hours. The highest concentration of cysticercoides occurs in the region mm from the pyloric sphincter. At about 102 hours, cysticercoids break out of the villi, envaginate and become attached in the posterior third of the gut. The prepatent period varies somewhat between hosts, being days in the rat (Shorb, 1933) and days in mice (Hunninen, 1935). In rats, maximum eggs production occurs between 14 and 16 days. The life span is short, days in rats and 2-56 days in mice. Species Used as Host Fleas, Siphonaptera Flour beetles, Tenebrionidae Caprophagus insects, Insecta Rodents, Rodentia (57)

4 GUPTA ET AL. Humans, Homo sapiens DISCUSSION H. nana was rst identi ed as a human parasite by Von Siebold in In 1906, Stiles (cf Scott and Camb, 1923) identi ed an identical parasite with a rodent host and named it Hymenolepis fraterna. Later, morphological characteristics were used for taxonomy identi cation and H. nana was known to have hooks and linear reproductive organs. H. diminuta has no hooks and reproductive organs arranged in a triangular formation. Some workers are of the opinion that this species should be renamed Vampirolepis nana but this designation is not widespread use. Often referred to as 'dwarf' tapeworm, this is a common parasite of rodents and also, of man, in whom it causes hymenolepiasis. Morphologically, it differs from H. diminuta chie y by the presence of well developed rostellum armed with a crown of hooks. H. fusa was reported from India in birds by Southwell (1930). Bilqees and Siddiqui (1981) reported a similar cestode from rats in Karachi and identi ed it as H. fusa based on characters similar to H. fusa. H. nana infects the small intestine of rats. The present ndings are in accordance to earlier reports from India and abroad. The prevalence of the worm was 22% in UK (Webster and Macdonald, 1995), 31.3% in Iran (Sadjjadi and Massoud, 1999) whereas in the present case, the prevalence was quite low (6.15%). This tapeworm is signi cant in the sense that it is capable of causing infection in humans too and thus has a zoonotic importance. HAZARDS TO HUMANS Hymenolepis nana is regarded as the most common cestode parasite found in humans (Roberts and Janovy, 2000). It can frequently be found in the intestines of humans and absorb nutrients from the intestinal lumen (Cameron, 1956). In adults, the parasite is mainly a nuisance instead of a health problem, but in children, its pathological manifestations may become dangerous. Usually it is the larva of this tapeworm that causes the most problem in children (Lapage, 1951). The larva burrows into the walls of the intestine, if there are enough tapeworms in the child, severe damage can be in icted. This is done by absorbing all the nutrition from the food the child eats (Lapage, 1951). Hymenolepis nana usually does not cause deaths unless in severe infectivity and usually young children or in people who have weakened immune systems, the pathogenecity may be high. In some parts of the world, heavily infected individuals are a result of internal autoinfection (Lapage, 1951; Cameron, 1956; Olsen, 1974; Roberts and Janovy Jr., 2000). Patients with more than 15,000 eggs per gram of stool may experience cramps, diarrhoea, irritability, anorexia, or enteritis caused by cystercoids destroying the intestinal villi in which they develop. Symptoms include anal itching, diarrhcea (can be bloody), headache, increased appetite or loss of appetite, insomnia, muscle spasms, nausea, nervousness, seizures, stomach ache, vomiting, weakness, weight loss. In human adults, the tapeworm is more of a nuisance than a health problem, but in small children, many H. nana can be dangerous. It can be concluded that in order to improve the wealth of information on the range and variation in the component community structures of the intestinal parasites in wild Indian rats from different regions of the world and from different climatic zones, the study should be taken up by more research groups across the country. The important position occupied by these animals in biocenoses, their distribution, population density, the fact that this species cohabitates with humans, and the insigni cant knowledge of their gastrointestinal parasites in India, indicates the necessity of further investigations. REFERENCES. Bilqees, F.M. and Siddiqui, I.H Two cestodes from rats in Karachi. Pak. J. Agri. Res. 2(1): Cameron, T Parasites and Parasitism. NY: John Wiley and Sons, Inc.. Ghaffar, A "Cestodes" (On-line). at Goswami, R., Singh, S.M., Kataria, M. and Somvanshi, R Clinicopathological studies on spontaneous Hymenolepis diminuta infection in wild and laboratory rats. Braz. J. Vet. Pathol. 4(2): (58

5 PROC. ZOOL. SOC. INDIA Gupta, Neelima, Gupta, D.K. and Sharma P. K Condition factor and organosomatic indices of parasitized Rattus rattus as indicators of host health. J. Par. Dis. DOI /s Gupta, Neelima, Sharma P. K. Gupta, D.K. and Shalaby S.I Haemato-clinical changes in pregnant and non-pregnant rats, Rattus rattus Linnaeus, 1758 under parasitic stress. Egypt. J. Vet. Sci. 44(1): Gupta, S, Katiyar, J.C. and Sen, A.B Effect of mode of infection on the development and chemotherapeutic response of Hymenolepis nana in rats. J. Helminthol. 55: doi: /s x Henderson, D.J and Hanna, R.E.B Hymenolepis nana (Cestoda: Cyclophyllidae): migration, growth and development in the laboratory mouse. Int. J. Parasitol. 17: Hughes, R.C The genus Hymenolepis Weinland, Oklahoma Agric. Exp. Stn. Tech. Bull. 8:42. Hunninen, A.V Studies on the life history and host-parasite relsations of Hymenolepis fraternal (H. nana var. fraterna Stiles) in white mice. Am. J. Hyg. 22: Kumar, Sudheer, Gupta, D.K. and Gupta, Neelima Haemoglobinopathy in wild rats, Rattus rattus (Linnaeus) infected with a cestode parasite, Hymenolepis diminuta (Rudolphi). Eur. J. Pharm. Med. Res. 3(5): Kumazawa, H. and Suzuki, N Kinetics of proglottid formation, maturation and shedding during development of Hymenolepis nana. Parasitology. 86: Lapage, G Parasitic Animals. Great Britain: The University Press. Lapage, G Veterinary Parasitology. Oliver and Boyd. Edinburgh. Tweedale court, London. 39-A Welbeck Street W.I. Mirdha, B.R. and Samantray, J.C Hymenolepis nana: a common cause of paediatric diarrhoea in urban slum dwellers in India. J. Trop. Pediatr. 48(6): Olsen, O Animal Parasites, Third Edition. MD: University Park Press. Roberts, L., J. Janovy Jr Foundations of Parasitology, Sixth Edition. MA: Mcgraw-Hill Higher Education Sadjjadi, S.M. and Massoud, J Helminth parasites of wild rodents in Khuzestan Province, Southwest of Iran. J. Vet. Parasitol. 13: Scott, H.H. and Camb, H A contribution to the experimental study of the life-histories of Hymenolepis fraterna Stiles, 1906, and Hymenolepis longior Baylis, 1922, in the mouse. J.Helminthol. 1: doi: /s x Shorb, D.A Host-parasite relations of Hymenolepis fraterna in the rat and the mouse. Am. J. Hyg. 18: Smyth, J.D Introduction to Animal Parasitology. The English Univ. Press Ltd., 102 New Gate Street, London. Southwell, T The Fauna of British India including Ceylon and Burma. Cestoda Vol I. Taylor and Francis. Red Lion Court, Fleet Street, London. Webster, J.P. and Macdonald, D.W Parasites of wild brown rats (Rattus norvegicus) on UK farms. Parasitology, 111: Yamaguti, S Systema Helminthum Vol. II. The Cestodes of Vertebrates. Interscience Publishers Inc. New York. (59)

6 GUPTA ET AL. (60)

7 PROC. ZOOL. SOC. INDIA (61)

WITH THE TABLE OF THE MORPHOLOGICAL FEATURES OF TAPEWORMS IN VAMPIROLEPIS. (Received: December 22nd, 1965)

Japan. J. Med. Sci. Biol. 19, 51-57, 1966 *ON A NEW TAPEWORM, VAMPIROLEPIS ISENSIS, FOUND IN BATS WITH THE TABLE OF THE MORPHOLOGICAL FEATURES OF TAPEWORMS IN VAMPIROLEPIS ISAMU SAWADA Biological Laboratory,