Research note on the anti-sera in the Japanese quail. Yoshinobu Tanaka, Noborur Wakasugi and Takeshi Tomita

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1 Research Note Research note on the anti-sera in the Japanese quail Yoshinobu Tanaka, Noborur Wakasugi and Takeshi Tomita Laboratory of Animal Breeding, Faculty of Agriculture, Nagoya University, Furo-cho, Chiguss-ku, Nagoya Ten quail stocks which were maintained by the rotation system were used in this study. General care of the quails and the method for maintenance of the stocks have been described elsewhere (Nakasugi and Kcndo, 1971). Fourteen pairs (donor - host) were arranged for immunization and six pairs produced anti-sera (Table 1). Table 1 Donor - host pairs which produced antibodies Table 2 shows frequency of positive reaction to each antiserum in each quail stock. Anti-A and anti-b sera had similar characteristics. Anti-E and anti-f sera had also similar characteristics. Genetic analysis suggested that antigenisity to these 21

2 anti-sera was controlled by the respective autosomal dominant genes. Table 2 Frequency of positive reaction in each quail stock Ref eren e Noboru Wakasugi and Kyo ji Kondo (1971) Breeding methods for maintenance of mutant genes and establishment of strains in the Japanese quail Proceedings of the ICLA Asinan Pacific meeting on laboratory animalsy Tokyo and Inuyama, September (in press) 22

3 Strains for the preparations of blood grouping antisera in the chicken Yoshihisa Fujio Laboratory of Animal Genetics, Faculty of Agriculture, Nagoya University, Chigusa-ku, Nagoya It is difficult to obtain the large quantities of the specific isoimmune sera and the reproducibility to obtain the specific isoimmune serum from different chickens is doubtful. To solve the above problems, the strains of which the birds were homozygous for the genes determining blood group antigens would be desirable. The strains established are summarized in Table to The homozygosity was elucidated from the fact that there was no segregation of chickens in regard to antigen types in these strains through more than three generations. Table 1. The blood group antigens of strains of chicken 23

4 Antisera were obtained easily by isoimmunization between these strains. Then, an absorption was performed to obtain the antiserum specific for each antigen. A new agglutinogen detected with soy bean phytohemagglutinin in Japanese quail. Makoto Mizutani NIBS Laboratory Animal Research Station A new agglutinogen was detected with the seed extract of Glycine max (soy bean phytohemagglutinin) in Japanese quail red cells. The agglutinin was active at cold temperature(4 Ž), suggesting it to be cold agglutinin. The agglutinogen designated as gsb h was detected on the red cells of males and non-laying females, but not on red cells of laying females. Among laying females, there was the segregation of individuals showing agglutinable. Two strains, agglutinable (JqNIBS-SB+) and non-agglutinable(jgnibs-sb-), were established from the segregating population. Data on the mating of agglutinable strain with non-aggiutinable strain suggests that disappearance of gsb h agglutinogen in laying status is under the control of a single gene. In embryonic observation, gsb h agglutinogen was found on the red cells of embryos regardless of sex and strain. The agglutinability of red cells was maintained from 7th day embryos to adults but decreased with laying and lost in nonagglutinable strain. When 3 weeks old males and females were injected with diethylstilbesterol, the agglutinability was lost in non-agglutinable strain but not in agglutinable strain. In the connection on gph h agglutinogen in chicken, the study 24

5 on this agglutinogen is of interest. Table 1. The agglutinability of quail red cells with soy bean phytohemagglutinin * All female birds were laying The red cell agg!utinogen detected with peanut phytohemagglutinin in chicken and Japanese quail Makoto Mizutani NIBS Laboratory Animal Research Station The agglutinogen of chicken and quail red cells was detected with the peanut extract (peanut phytohemagglutinin). In the chicken and quail populations, there was the segregation of individuals showing agglutinable and non-agglutinable. The agglutinogen designated as gpn h was detected at 5 weeks old chicks and newly hatched quail. Absorption test suggests that the same agglutinogen was controlled by an autosomal dominant gene. In quail, the selection for the agglutinability was carried out and two strains were established, agglutinable (JqNIBS-Pn+) and non-agglutinable (JqNIBS-Pn-) strain, The result on the mating of agglutinable strain with non-agglutin- 25

6 able strain indicated that the agglutinogen was controlled bar an autosomal dominant gene(pn). Table 1. Relationship between the red cell agglutinogen of chicken and quail * Number represents agglutination score Table 2. Genetic analysis of gpn h agglutinogen in Japanese quail 26

7 A new agglutinogen detected with phytohemagglutinin in pigeon Makoto Mizutani NIBS Laboratory Animal Research Station The agglutinogen of pigeon red coils was detected by the seed extract of Pisum sativum (garden peas phytohemagglutinin). The agglutinogen designated as gph h was found in adult females but not in adult males. The agglutinability was examined in the red cells from one week old, two weeks old, three weeks old, and four weeks old birds. There was the segregation of birds showing agglutinable and non-agglutinable regardless of sex in one week old birds. The agglutinability was decreased with the growth. The data on progeny test suggests that the agglutinogen may be controlled by an dominant gene (Table 1). When adult males were injected with diethylstilbestrol, the agglutinability was shown. This phenomenon is interesting to study in the connection on gph h agglutinogen in chicken. Table 1. Progeny test * The red cells from one week old birds were tested. 27